ORBi: Califice Sophie - A single ancient origin for prototypical SR splicing factors

Reference : A single ancient origin for prototypical SR splicing factors


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/106962

Title :	A single ancient origin for prototypical SR splicing factors
Language :	English
Author, co-author :	Califice, Sophie [> >]
	Baurain, Denis [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale > >]
	Hanikenne, Marc [Université de Liège - ULg > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
	Motte, Patrick [Université de Liège - ULg > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Publication date :	Feb-2012
Journal title :	Plant Physiology
Publisher :	American Society of Plant Biologists
Volume :	158
Issue/season :	2
Pages :	546-560
Audience :	International
ISSN :	0032-0889
e-ISSN :	1532-2548
City :	Rockville
Country :	MD
Keywords :	[en] splicing ; SR protein ; Phylogeny
Abstract :	[en] Eukaryotic pre-mRNA splicing is a process involving a very complex RNA-protein edifice. Serine/arginine-rich (SR) proteins play essential roles in pre-mRNA constitutive and alternative splicing, and have been suggested to be crucial in plant-specific forms of developmental regulation and environmental adaptation. Despite their functional importance, little is known about their origin and evolutionary history. SR splicing factors have a modular organization featuring at least one RRM domain and a C-terminal region enriched in Ser/Arg dipeptides. To investigate the evolution of SR proteins, we infer phylogenies for >12,000 RRM domains representing >200 broadly sampled organisms. Our analyses reveal that the RRM domain is not restricted to eukaryotes and that all prototypical SR proteins share a single ancient origin, including the plant-specific SR45 protein. Based on these findings, we propose a scenario for their diversification into four natural families, each corresponding to a main SR architecture, and a dozen subfamilies, of which we profile both sequence conservation and composition. Finally, using operational criteria for computational discovery and classification, we catalogue SR proteins in 20 model organisms, with a focus on green algae and land plants. Altogether, our study confirms the homogeneity and antiquity of SR splicing factors, while establishing robust phylogenetic relationships between animal and plant proteins, which should enable functional analyses of lesser characterized SR family members, especially in green plants.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/106962
DOI :	10.1104/pp.111.189019
Other URL :	http://www.plantphysiol.org/content/158/2/546.long

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Fulltext file(s):

	File	Commentary	Version	Size	Access
Open access	Califice_et_al_2012_Plant_Physiol_postprint_editor.pdf		Publisher postprint	1.62 MB	View/Open

Additional material(s):

	File	Commentary	Version	Size	Access
Open access	189019suppdata.pdf	Sup. mat.	-	4.9 MB	View/Open
Open access	189019_DS1.xls		-	173 kB	View/Open
Open access	189019_DS2.doc		-	120.5 kB	View/Open
Open access	189019_DS3.pdf		-	5.08 MB	View/Open
Open access	189019supptbl.xls		-	1.16 MB	View/Open
Open access	189019_DS4.xls		-	1.18 MB	View/Open

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