ORBi: Kesteloot Frédéric - Follow-up of COLL2-1, COLL2-1NO2 and myeloperoxydase in dogs after transection of the cruciate ligament of the knee

Reference : Follow-up of COLL2-1, COLL2-1NO2 and myeloperoxydase in dogs after transection of the cr...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Human health sciences : Rheumatology
	To cite this reference:	http://hdl.handle.net/2268/10641

Title :	Follow-up of COLL2-1, COLL2-1NO2 and myeloperoxydase in dogs after transection of the cruciate ligament of the knee
Language :	English
Author, co-author :	Kesteloot, Frédéric [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
	Pelletier, Jean-Pierre [Université de Montréal - UdeM > Centre hospitalier Notre-Dame > Unité de Recherche sur l'Arthrose > >]
	Martel-Pelletier, Johanne [Université de Montréal - UdeM > Centre hospitalier Notre-Dame > Unité de Recherche sur l'Arthrose > >]
	Caron, Judith [Université de Montréal - UdeM > Centre hospitalier Notre-Dame > Unité de Recherche sur l'Arthrose > >]
	Boileau, Christelle [Université de Montréal - UdeM > Centre hospitalier Notre-Dame > Unité de Recherche sur l'Arthrose > >]
	Bosseloir, Alain [Zentech - Liège > > > >]
	Msika, Philippe [Laboratoires Expanscience - Courbevoie - FRA > > > >]
	Guillou, Bernard [Laboratoires Expanscience - Courbevoie - FRA > > > >]
	Henrotin, Yves [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie >]
	Deberg, Michelle [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Publication date :	23-May-2009
Peer reviewed :	Yes
Audience :	International
Event name :	ICRS 2009
Event date :	du 23/05/2009 au 26/05/2009
Event organizer :	International Cartilage Repair Society
Event place (city) :	Miami
Event country :	USA
Abstract :	[en] Purpose: To determine the profile of Coll2-1, Coll2-1NO2 and myeloperoxydase (MPO) serum concentrations in experimental knee OA induced in the dog by transection of the anterior cruciate ligament. Methods: Surgical transection of the ACL of the right knee was performed on 16 adult crossbred dogs. The dogs were sacrificed 8 weeks after the surgical procedure. Coll2-1, Coll2-1NO2 and MPO were measured by specific immunoassays in 16 dogs at baseline and every 2 weeks during the 8 weeks. The results were expressed as median (range). Results: Immunostainings with D3 and D37, the antiserum recognizing Coll2-1 and Coll2-1NO2, respectively, labelled extracellular matrix in the superficial layer of fibrillated cartilage. After the transection of the ACL, the concentration of 3 biomarkers increased significantly (Friedman test: p<0.001). The concentrations of Coll2-1 and MPO were significantly increased at week 2 compared to baseline [Coll2-1 baseline: 281.57 (131.02-384.67) nM vs Coll2-1 week 2: 345.52 (181.15-589.25) nM (p<0.01) and MPO baseline: 5.16 (<0.4-14.7) ng/ml vs MPO week 2: 14.54 (3.28-31.50) ng/ml (p<0.001)] and remained stable until week 8 [Coll2-1 week 8:318.89 (117.95-492.28) nM and MPO week 8: 11.55 (2.87-42.94) ng/ml]. The Coll2-1NO2 concentration increased significantly at weeks 6 and 8 compared to baseline [Coll2-1NO2 baseline: 0.54 (0.29-1.48) nM vs Coll2-1NO2 week 6: 0.64 (0.40-1.9) nM (p<0.001) vs week 8: 0.61 (0.37-1.79) nM]. Conclusions: These findings suggest that Coll2-1 is a relevant marker for the detection of early structural changes in OA dogs. Interestingly, MPO and Coll2-1NO2 are increased in OA dogs indicating that an oxidative stress occurs in this OA model.
Permalink :	http://hdl.handle.net/2268/10641

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