Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.
Nguyen, V. H.; Barbato, O.; Bui, X. N.et al.
2012 • In Animal Science Journal, 83 (10), p. 683-689
[en] Pregnancy-associated glycoproteins (PAG) constitute a large family of glycoproteins found in the outer placental epithelial cell layer of the placenta in Eutherian species. In ruminants, they are noted to be structurally closely related among the different species. This study was designed to determine PAG concentrations in maternal and fetal plasma, allantoic and amniotic fluids in buffalo species. Antisera (AS) generated in rabbits against distinct PAG molecules were used in three RIA-PAG systems: RIA-1 (raised against bovine PAG67kDa; AS#497), RIA-2 (raised against caprine PAG55+62kDa; AS#706) or RIA-3 (raised against buffalo PAG; AS#859). Samples were collected at slaughterhouse (n = 67). PAG concentrations determined by RIA-2 gave significantly higher results in both allantoic and amniotic fluids (12.7 ± 2.1 ng/ml and 24.0 ± 7.3 ng/ml, respectively). Regarding maternal and fetal plasma, PAG concentrations obtained by RIA-2 (21.8 ± 2.4 ng/ml and 20.2 ± 2.5 ng/ml, respectively) and RIA-3 (25.0 ± 2.2 ng/mL and 21.9 ± 3.2 ng/ml, respectively) were higher than those obtained by RIA-1 (15.5 ± 1.4 ng/ml and 16.1 ± 1.8 ng/ml, respectively). The correlation among the three systems was very high. The study clearly reveals the ability of PAG-RIA systems to determine PAG concentration in swamp buffalo samples.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Nguyen, V. H.
Barbato, O.
Bui, X. N.
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Assessment of pregnancy-associated glycoprotein (PAG) concentrations in swamp buffalo samples from fetal and maternal origins by using interspecies antisera.
Publication date :
October 2012
Journal title :
Animal Science Journal
ISSN :
1344-3941
eISSN :
1740-0929
Publisher :
Blackwell Publishing, Japan
Volume :
83
Issue :
10
Pages :
683-689
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Belgian Technical Cooperation (to N. Van Hanh), FNRS (Grant number CC 1.5.059.08) and Ministry of the Walloon Region-DGA (Grant number D31-1184)
Ayad A, Sousa NM, Sulon J, Hornick JL, Iguer-Ouada M, Beckers JF. 2009. Correlation of five radioimmunoassay systems for measurement of bovine plasma pregnancy-associated glycoprotein concentrations at early pregnancy period. Research in Veterinary Science 86, 377-382.
Ayad A, Sousa NM, Sulon J, Iguer-ouada M, Beckers JF. 2007. Comparison of five radioimmunoassay systems for PAG measurement: ability to detect early pregnancy in cows. Reproduction in Domestic Animals 42, 433-440.
Barbato O, Sousa NM, Klisch K, Clerget E, Debenedetti A, Barile VL, Malfatti A, Beckers JF. 2008. Isolation of new pregnancy-associated glycoproteins from water buffalo (Bubalus bubalis) placenta by Vicia villosa affinity chromatography. Research in Veterinary Science 85, 457-466.
Barbato O, Sousa NM, Malfati A, Debenedetti A, Todini L, Barile VL, Beckers JF. 2009. Concentrations of pregnancy-associated glycoproteins in Water buffaloes females (Bubalus bubalis) during pregnancy and postpartum periods. In: O'Leary M, Arnett J (eds), Pregnancy Protein Research, pp. 123-134. Nova Science Publishers, Washington, DC.
Beckers JF, De Coster R, Wouters-Ballman P, Fromont-Liénard C, Van Der Zwalmen P, Ectors F. 1982. Dosage radioimmunologique de l'hormone placentaire somatotrope et mammotrope bovine. Annales de Médecine Vétérinaire 126, 9-21.
Borghese A, Mazzi M. 2005. Buffalo population and strategies in the world. In: Borghese A (ed.), Buffalo Production and Research, pp. 1-39. FAO Press, Rome.
Butler JE, Hamilton WC, Sasser RG, Ruder CA, Hass GM, Williams RJ. 1982. Purification and characterization of a bovine pregnancy-associated glycoprotein. Biology of Reproduction 45, 1-10.
Byatt JC, Wallace CR, Bremel RD, Collier RJ, Bolt DJ. 1987. The concentration of bovine placental lactogen and the incidence of different forms in fetal cotyledons and in fetal serum. Domestic Animal Endocrinology 4, 231-241.
Cruz LC. 2010. Recent developments in the buffalo in industry of Asia. In: Proceeding 9th Buffalo congress. Buenos Aires, Argentina: 7-19.
Debenedetti A, Sousa NM, Sulon J, Beckers JF, Barbato O, Malfatti A. 2001. Pregnancy-associated glycoprotein detection by RIA in pregnant buffalo cows (Bubalus bubalis). Biotechnology, Agronomy, Society and Environment 5 (Suppl 5), 62. (Abstract 35).
El Amiri B, Remy B, de Sousa NM, Beckers JF. 2004. Isolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation. Reproduction Nutrition Development 44, 169-181.
El Amiri B, Remy B, Sousa NM, Joris B, Gerardin-Otthiers N, Perenyi Z, Banga-Mboko H, Beckers JF. 2003. Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta. Molecular Reproduction and Development 64, 199-206.
El-Battawy KA, Sousa NM, Szenci O, Beckers JF. 2009. Pregnancy-associated glycoprotein profile during the first trimester of pregnancy in Egyptian buffalo cows. Reproduction in Domestic Animals 44, 161-166.
Garbayo JM, Green JA, Manikkam M, Beckers JF, Kiesling DO, Ealy AD, Roberts RM. 2000. Caprine pregnancy-associated glycoproteins (PAG): their cloning, expression, and evolutionary relationship to other PAG. Molecular Reproduction and Development 57, 311-322.
Garbayo JM, Remy B, Alabart JL, Folch J, Wattiez R, Falmagne P, Beckers JF. 1998. Isolation and partial characterization of a pregnancy-associated glycoprotein (PAG) family from the goat placenta. Biology of Reproduction 58, 109-115.
Garbayo JM, Serrano B, Lopez-Gatius F. 2008. Identification of novel pregnancy-associated glycoproteins (PAG) expressed by the peri-implantation conceptus of domestic ruminants. Animal Reproduction Science 103, 120-134.
Gonzalez F, Sulon J, Garbayo JM, Batista M, Cabrera F, Calero P, Gracia A, Beckers JF. 1999. Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentration in plasma samples. Theriogenology 53, 717-725.
Green JA, Xie S, Quan X, Bao B, Gan X, Mathialagan N, Beckers JF, Roberts RM. 2000. Pregnancy-associated bovine and ovine glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biology of Reproduction 62, 1624-1631.
Greenwood FC, Hunter WM, Glover JS. 1963. The preparation of 131I labeled human growth hormone of high specific radioactivity. Biochemical Journal 89, 114-123.
Guruprasad K, Blundell TL, Xie S, Green JA, Szafranska B, Nagel RJ, Mcdowell K, Baker CB, Roberts RM. 1996. Comparative modeling and analysis of amino acid substitutions suggests that the family of pregnancy-associated glycoproteins includes both active and inactive aspartic proteinases. Protein Engineering 94, 849-856.
Huang F, Cockrell DC, Stephenson TR, Noyes JH, Sasser RG. 1999. Isolation, purification, and characterization of pregnancy-specific protein B from elk and moose placenta. Biology of Reproduction 61, 1056-1061.
Karen A, Darwish S, Ramoun A, Tawfeek K, Van Hanh N, de Sousa NM, Sulon J, Szenci O, Beckers JF. 2007. Accuracy of ultrasonography and pregnancy-associated glycoprotein test for pregnancy diagnosis in buffaloes. Theriogenology 68, 1150-1155.
Kiewisz J, Sousa NM, Beckers JF, Panasiewicz G, Gizejewski Z, Szafranska B. 2009. Identification of multiple pregnancy-associated glycoproteins (PAGs) purified from the European bison (Eb; Bison bonasus L.) placentas. Animal Reproduction Science 112, 229-250.
Kiewisz J, Sousa NM, Beckers JF, Vervaecke H, Panasiewicz G, Szafranska B. 2008. Isolation of pregnancy-associated glycoproteins from placenta of American bison (Bison bison) at first half of pregnancy. General and Comparative Endocrinology 155, 164-175.
Klisch K, de Sousa NM, Beckers JF, Leiser R, Pich A. 2005. Pregnancy associated glycoprotein-1, -6, -7, and -17 are major products of bovine binucleate trophoblast giant cells at midpregnancy. Molecular Reproduction and Development 71, 453-460.
Ledezma-Torres RA, Beckers JF, Holtz W. 2006. Assessment of plasma profile of pregnancy-associated glycoprotein (PAG) in sheep with a heterologous (anti-caPAG(55+59)) RIA and its potential for diagnosing pregnancy. Theriogenology 66, 906-912.
Perenyi Z, Szenci O, Sulon J, Drion PV, Beckers JF. 2002. Comparison of the ability of three radioimmunoassays to detect pregnancy-associated glycoproteins in bovine plasma. Reproduction in Domestic Animals 37, 100-104.
Riding GA, Hill JR, Jones A, Holland MK, Josh PF, Lehnert SA. 2008. Differential proteomic analysis of bovine conceptus fluid proteins in pregnancies generated by assisted reproductive technologies. Proteomics 8, 2967-2982.
Sousa NM, Remy B, El Amiri B, Figueiredo JR, Banga-mboko H, Dias goncalves PB, Beckers JF. 2002. Characterization of pregnancy-associated glycoproteins extracted from zebu (Bos indicus) placentas removed at different gestational periods. Reproduction Nutrition Development 42, 227-241.
Sousa NM, Szenci O, Taverne MAM, van der Weijden B, Beckers JF. 2008. Pregnancy-associated glycoproteins and their importance in monitoring the progress of bovine pregnancy. Hungarian Veterinary Journal 130 (Suppl 1), 8-11.
Van Hanh N, Huu QX, Uoc NT, Sulon J, Sousa NM, Nguyen BX, Beckers JF. 2006. Determination of pregnancy-association glycoproteins (PAGs) in swamp buffaloes (Bubalus bubalis) by radioimmunoassay. In: Proceedings of the 3rd Annual Conference, Asian Reproductive Biotechnology Society. Hanoi, Vietnam; 162-163.
Xie S, Green JA, Bao B, Bixby JB, Szafranska B, Demartini JC, Hecht S, Roberts RM. 1997b. The diversity and evolutionary relationships of the pregnancy-associated glycoproteins, an aspartic proteinase subfamily consisting of many trophoblast-expressed genes. Proceedings of the National Academy of Sciences of the United States of America 94, 12809-12816.
Xie S, Low BG, Nagel RJ, Kramer KK, Anthony RV, Zoli AP, Beckers JF, Roberts Xie S, Green JA, Bao B, Beckers JF, Valdez KE, Hakami L, Roberts RM. 1997a. Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue. Biology of Reproduction 57, 1384-1393.
Xie SC, Low BG, Nagel RJ, Kramer KK, Anthony RV, Zoli AP, Beckers JF, Roberts RM. 1991. Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proceedings of the National Academy of Sciences of the United States of America 88, 10247-10251.
Zoli AP, Beckers JF, Wouters-ballman P, Closset J, Falmagne P, Ectors F. 1991. Purification and characterization of a bovine pregnancy-associated glycoprotein. Biology of Reproduction 45, 1-10.
Zoli AP, Guilbault LA, Delahaut P, Benitez Ortiz W, Beckers JF. 1992. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biology of Reproduction 46, 83-92.
