Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows

Gajewski, Z.; Melo de Sousa, Noelita; Beckers, Jean-François; Pawlinski, B.; Olszewska, M.; Thun, R.; Kleczkowski, M.

Article (Scientific journals)

Gajewski, Z.; Melo de Sousa, Noelita; Beckers, Jean-François et al.

2008 • In Journal of Physiology and Pharmacology, 59 (Suppl 9), p. 55-64

Permalink
https://hdl.handle.net/2268/106226

PubMed
19261971

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

GajewskiZ_JPhysiolPharmacol_2008.pdf

Publisher postprint (200.66 kB)

Request a copy

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Pregnancy-associated glycoprotein; Cattle; Milk; Radioimmunoassay; Pregnancy diagnosis

Abstract :

[en] Pregnancy diagnosis is an important part in reproduction management of ruminants. The aim of the study was to use a new method for evaluating the bPAG and cPAG in milk and blood bPAG and compare this results with the other method for pregnancy diagnosis in the cows. The study was carried out in 220 Holstein Frisian cows. Heparinised blood samples were taken from the jugular vein and stored at -20 degrees C until PAG assay by RIA. For bPAG and cPAG, RIA test, milk samples were homogenized. Pure bPAG was used as a standard tracer described by Zoli et al. (1992). The cows were diagnosed as pregnant by means of USG (Aloka SSD 210) and by rectal palpation. bPAG and cPAG concentration in milk increased after 28 day of pregnancy and showed the rapid increase near the parturition. The same results of bPAG concentration we obtained in the blood samples. The decline of bPAG concentration was faster in the milk than in the blood. The data showed that the RIA method is precise enough to measure PAG concentrations in the maternal blood and milk of cows. The data indicate that milk samples can be used for pregnancy diagnosis in cows. The sensitivity and specificity of RIA measurement of PAG are very high.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Gajewski, Z.

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Pawlinski, B.

Olszewska, M.

Thun, R.

Kleczkowski, M.

Language :

English

Title :

Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows

Publication date :

2008

Journal title :

Journal of Physiology and Pharmacology

ISSN :

0867-5910

eISSN :

1899-1505

Publisher :

Polish Physiological Society, Krakow, Poland

Volume :

Issue :

Suppl 9

Pages :

55-64

Available on ORBi :

since 26 December 2011

Statistics

Number of views

61 (0 by ULiège)

Number of downloads

2 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

Bibliography

Humblot, P., Camous, S., Martal, J., Pregnancy specific protein B, progesterone concentrations and embryonic mortality during early pregnancy in dairy cows (1988) J Reprod Fertil, 83, pp. 215-223
Zoli, A.P., Guilbault, L.A., Delahaut, P., Benitez-Ortiz, W., Beckers, J.F., Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: Its application in pregnancy diagnosis (1992) Biol Reprod, 46, pp. 83-92
Szenci, O., Taverne, M.A., Beckers, J.F., Evaluation of false ultrasonographic diagnoses in cows by measuring plasma levels of bovine pregnancy-associated glycoprotein 1 (1998) Vet Rec, 142, pp. 304-306
Szenci, O., Beckers, J.F., Humblot, P., Comparison of ultrasonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glycoprotein 1 tests for pregnancy detection in dairy cows (1998) Theriogenology, 50, pp. 77-88
Melo De Sousa, N., Ayad, A., Beckers, J.F., Gajewski, Z., PAG as a pregnancy marker in ruminants (2006) J Physiol Pharmacol, 57, pp. 153-172
Tainturier, D., Bdel, M., Beckers, J.F., Fieni, F., Cinetique de la bPAG dans le plasma et dans le lait au cours des trois semaines suivant le part chez la vache laitere (1996) Actualite Scientifique-Reproduction et Production Latitiere, pp. 129-133. , Ed Aupelf-Uref
Gonzalez, F., Sulon, J., Calero, P., Batista, M., Gracia, A., Beckers, J.F., Pregnancy Associated Glycoproteins (PAG) detection in milk samples for pregnancy diagnosis in dairy goat (2001) Teriogenology, 56, pp. 671-676
El Amiri, B., Cognie, Y., Sulon, J., Pregnancy-associated glycoprotein concentrations in plasma and milk samples for early pregnancy diagnosis in Lacaune dairy sheep (2003) Reprod Domest Anim, 38 (4), p. 322
Lopez-Gatius, F., Garbayo, J., Santolaria, P., Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses (2006) Dom Anim Endocrinol, 32 (1), pp. 29-42
Gajewski, Z., Beckers, J.F., Melo De Sousa, N., Thun, R., Sulon, J., Faundez, R., Determination of Pregnancy Associated Glycoprotein concentrations in sheep a retrospective study (1999) Adv in Cell Biology, 26, pp. 89-96
El Amiri, B., Karen, A., Sulon, J., Measurement of Ovine Pregnancy-Associated Glycoprotein (PAG) during Early Pregnancy in Lacaune Sheep (2007) Reprod Dom Anim, 42, pp. 257-262
Mialon, M.M., Camous, S., Renand, G., Martal, J., Menissier, F., Peripheral concentrations of a 60-kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle (1993) Reprod Nutr Dev, 33, pp. 269-282
Szenci, O., Taverne, M.A.M., Beckers, J.F., Sulon, J., Varga, J., Borzsonyi, L., Hanzen, Ch., Schekk, Gy., Evaluation of false ultrasonographic diagnoses in cows by measuring plasma levels of bovine pregnancy-associated glycoprotein 1 (1998) Veterinary Record, 142 (12), pp. 304-306
Sasser, R.G., Ruder, C.A., Ivani, K.A., Butler, J.E., Hamilton, W.C., Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation (1986) Biol Reprod, 35, pp. 936-942
Humblot, P., Camous, S., Martal, J., Diagnosis of pregnancy by radioimmunoassay of a pregnancy-specific protein in the plasma of dairy cows (1988) Theriogenology, 30, pp. 257-268
Breukelman, S.P., Perenyi, Z., Ruigh, L., Plasma concentrations of bovine pregnancy-associated glycoprotein (bPAG) do not differ during the first 119 days between ongoing pregnancies derived by transfer of in vivo and in vitro produced embryos (2005) Theriogenology, 63, pp. 1378-1389
Garbayo, J.M., Green, J.A., Manikkam, M., Caprine pregnancy-associated glycoproteins (PAG): Their cloning, expression, and evolutionary relationship to other PAG (2000) Mol Reprod Dev, 57, pp. 311-322
Sousa, N.M., Ayad, A., Beckers, J.F., Gajewski, Z., Pregnancy-associated glycoproteins (PAG) as pregnancy markers in the ruminants (2006) Journal of Physiology and Pharmacology, 57 (SUPPL. 8), pp. 153-171. , http://www.jpp.krakow.pl/journal/archive/1106_s8/pdf/153_1106_s8_article. pdf
Garbayo, J.M., Remy, B., Alabart, J.L., Isolation and partial characterization of a pregnancy-associated glycoprotein (PAG) family from the goat placenta (1998) Biol Reprod, 58, pp. 109-115
Metelo, R., Sulon, J., Moreira Da Silva, R., Beckers, J.F., Preliminary results for measuring bovine PAG in milk simples (2002) 7eme Journee de Rencontre Bioforum, p. 32. , BioLiege, Association des Biotechnologistes Liegeois Liege 3 May
Gajewski, Z., Melo De Sousa, N., Konopka, A., Determination of PAG in the milk and blood in cow's in comparison to other examination methods for pregnancy diagnosis (2008) Procedings XXV WBC Budapest, pp. 92-97
Butler, J.E., Hamilton, W.C., Sasser, R.G., Detection and partial characterization of two bovine pregnancy-specific proteins (1982) Biol Reprod, 26, pp. 925-933
Beckers, J.F., Dewulf, M., Verstegen, J., Wouters-Ballmann, P., Ectors F Isolation of a bovine chorionic gonadotrophin (bCG) (1988) Theriogenology, 29, p. 216
Zoli, A.P., Beckers, J.F., Wouters-Ballman, P., Closset, J., Falmagne, P., Ectors, F., Purification and characterization of a bovine pregnancy-associated glycoprotein (1991) Biol Reprod, 45, pp. 1-10
Vaitukaitis, J., Robbins, J.B., Nieschlag, E., Ross, G.T., A method for producing specific antisera with small doses of immunogen (1971) J Clin Endocrinol Metab, 33, pp. 988-991
Greenwood, F.C., Hunter, W.M., Glover, J.S., The preparation of 131-I labeled human growth hormone of high specific radioactivity (1963) J Biochem, 89, pp. 114-123
Zoli, A.P., Guilbault, L.A., Delabaut, P., Ortiz, W.B., Beckers, J.F., Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: Its application for pregnancy diagnosis (1992) Biol Reprod, 46, pp. 83-92
Garbayo, J.M., Remy, B., Alabart, J.L., Isolation and partial characterization of a pregnancy-associated glycoprotein (PAG) family from the goat placenta (1998) Biol Reprod, 58, pp. 109-115
Perenyi, Z.S., Szenci, O., Sulon, J., Drion, P.V., Beckers, J.F., Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma (2002) Reproduction in Domestic Animals, 37 (2), pp. 100-104. , DOI 10.1046/j.1439-0531.2002.00341.x
Perenyi, Z., (2002) Investigations on Pregnancy-associated Glycoproteins in the Cow, pp. 1-150. , PhD. Thesis University of Liege
Perenyi, Z., Szenci, O., Drion, P.V., Aspartic proteinase members secreted by the ruminant placenta: Specificity of three radioimmunoassay system for the measurement of pregnancy-associated glycoproteins (2002) Reprod Dom Anim, 37, pp. 324-329
Lopez-Gatius, F., Hunter, R.H., Garbayo, J.M., Plasma concentrations of pregnancy-associated glycoprotein-1 (PAG-1) in high producing dairy cows suffering early fetal loss during the warm season (2007) Theriogenology, 67, pp. 1324-1330
Sousa, N.M., Garbayo, J.M., Figueiredo, J.R., Sulon, J., Goncalves, P.B.D., Beckers, J.F., Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north-east of Brazil (1999) Small Ruminant Research, 32 (2), pp. 137-147. , DOI 10.1016/S0921-4488(98)00171-0, PII S0921448898001710
Patel, O.V., Sulon, J., Beckers, J.F., Plasma bovine pregnancy-associated glycoprotein concentrations throughout gestation in relationship to fetal number in the cow (1997) Eur J Endocrinol, 137, pp. 423-428
Chavatte-Palmer, P., Melo De Sousa, N., Laigre, P., Ultrasound fetal measurements and pregnancy associated glycoprotein secretion in early pregnancy in cattle recipients carrying somatic clones (2006) Theriogenology, 66, pp. 829-840
Lopez-Gatius, F., Santolaria, P., Yaniz, J., Rutllant, J., Lopez-Bejar, M., Factors affecting pregnancy loss from gestation day 38 to 90 in lactating dairy cows from a single herd (2002) Theriogenology, 57 (4), pp. 1251-1261. , DOI 10.1016/S0093-691X(01)00715-4, PII S0093691X01007154
Melo De Sousa, N., Zongo, M., Pitala, W., Pregnancy-associated glycoprotein concentrations during pregnancy and the postpartum period in Azawak zebu cattle (2003) Theriogenology, 59, pp. 1131-1142