Reference : An enzyme-linked immunoassay for direct measurement of the gelatin-binding capacity o...
Scientific journals : Article
Human health sciences : Anesthesia & intensive care
Human health sciences : Multidisciplinary, general & others
An enzyme-linked immunoassay for direct measurement of the gelatin-binding capacity of human plasma fibronectin
Damas, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Adam, A. [> > > >]
Closset, Jean mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques >]
Calay, G. [ > > ]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement >]
Lamy, M. [ > > ]
Foidart, J. [ > > ]
Mahieu, P. [ > > ]
Journal of Immunological Methods
Elsevier Science
Yes (verified by ORBi)
[en] Plasma fibronectin ; ELISA ; Gelatin-binding capacity
[en] A new solid-phase enzyme immunoassay measuring the gelatin-binding capacity of plasma fibronectin has been developed. This assay is based on the direct and high-affinity interaction between fibronectin and gelatin coated to polyvinyl chloride plates. The amount of fibronectin bound to gelatin is then measured by sequential incubation with a specific rabbit anti-human fibronectin antiserum, with horseradish peroxidase-conjugated goat anti-rabbit IgG antibodies and with substrate. The final degradation of the substrate is read at 492-650 nm in an ELISA processor. The assay allows the accurate detection of fibronectin concentrations ranging from 1 to 20 micrograms/ml, is inhibited by the addition of gelatin to plasma, is highly reproducible (interplate CV less than 10%), requires 100 microliter of plasma only and has been fully automated. Significant linear correlations were noted between total antigenic fibronectin (measured by laser nephelometry) and fibronectin gelatin-binding capacity in plasma from 310 blood donors. Both parameters were higher in men than in women and significantly increased according to age. Dissociation between immunoreactive fibronectin and fibronectin gelatin-binding capacity was observed in two polytraumatized patients. This enzyme immunoassay therefore provides a new method to investigate functional alterations of the gelatin-binding domain of fibronectin in various pathological conditions.

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