Reference : Amélioration du taux de multiplication in vitro de Jatropha curcas L.
Scientific journals : Article
Life sciences : Agriculture & agronomy
http://hdl.handle.net/2268/106043
Amélioration du taux de multiplication in vitro de Jatropha curcas L.
French
[en] Improvement of the rate of in vitro multiplication of Jatropha curcas L.
Medza Mve, Samson Daudet mailto [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
Mergeai, Guy mailto [Université de Liège - ULg > Sciences agronomiques > Phytotechnie tropicale et horticulture >]
Baudoin, Jean-Pierre mailto [Université de Liège - ULg > Sciences agronomiques > Phytotechnie tropicale et horticulture >]
Toussaint, André mailto [Université de Liège - ULg > Sciences agronomiques > Phytotechnie tropicale et horticulture >]
4-Oct-2010
Tropicultura
Agri-Overseas
28
4
200-204
Yes
International
0771-3312
Bruxelles
Belgium
[en] Jatropha curcas L ; Rate multiplication ; Accessions ; in vitro culture ; Nodal explants ; Cameroon ; Senegal ; Belgium
[en] In order to improve the in vitro multiplication rate (number of shoots/explant/subculture) of Jatropha curcas L. axillary nodes taken from young plants of two accessions of this species (originating from Cameroon and Senegal) have been cultivated for three weeks on a MS medium supplemented with 8,87 µM BAP, 4.92 µM IBA, and 30 g.l-1 sucrose at pH 5.7±0.1, and solidified with 0.7% agar. The shoots obtained from each original explant were then transferred to proliferation media (PM) consisting of MS medium supplemented with 2.21 to 8.9 µM BA or 2.21 to 8.9 µM kinetin in combination with 2.46 µM IBA. Each combination was completed with 33.12 µM adenine sulfate, 82.92 µM of glutamine and 30 g.l-1 sucrose. The best multiplication rate was obtained for the PM medium containing 6.65 µM BA and 2.46 µM AIB. On this medium 42.72±3.22 and 38.15±4.7 shoots/explant were obtained respectively for the accessions from Cameroon and Senegal after 6 weeks of culture, and the mean multiplication rates were 8.27±1.27 (accession from Cameroon) and 7.89±1.13 (accession from Senegal) shoots per explant during the 7 following subcultures (3 weeks/subculture). This medium was also the one that allowed the best overall growth in shoot height. Leafy shoots obtained have been rooted in a medium containing half of the major mineral components of MS supplemented with 5.7 µM IBA, 1.5% sucrose and solidified with 0.7% agar, then acclimated with a survival rate of 97%. These results allow considering the establishment of industrial units of plantlet multiplication from elite clones of J. curcas.
http://hdl.handle.net/2268/106043
also: http://hdl.handle.net/2268/150980
http://hdl.handle.net/2268/106043

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