Reference : Determination of a Suitable Protocol for Indigenous Oilseed Cucurbits Plant Regeneration
Scientific journals : Article
Life sciences : Agriculture & agronomy
http://hdl.handle.net/2268/106032
Determination of a Suitable Protocol for Indigenous Oilseed Cucurbits Plant Regeneration
English
[fr] Détermination d'un protocole adéquat pour la régérération de la cucurbite oléagineuse indigène
Gnamien, Yah Gwladys [> >]
Zoro Bi, I. A. [> >]
Djè, Y. [> >]
Toussaint, André mailto [Université de Liège - ULg > Sciences agronomiques > Phytotechnie tropicale et horticulture >]
Baudoin, Jean-Pierre mailto [Université de Liège - ULg > Sciences agronomiques > Phytotechnie tropicale et horticulture >]
2010
Tropicultura
Agri-Overseas
28
4
217-225
Yes
0771-3312
Bruxelles
Belgium
[en] Citrullus lanatus ; Cotyledon ; Edible Seeds ; Germplasm conservation ; Organogenesis ; Shoot regeneration ; Ivory Coast
[en] The present work was carried out to establish rapid in vitro propagation of the indigenous oilseed Citrullus lanatus. Efficiency of 3 protocols for seed decontamination was investigated. High level of seed sterilization was obtained after removing seed coat and soaking the seeds in 1.6 % sodium hypochlorite with a drop of Tween 20. Shoot tips, single node and cotyledon explants of 3 morphotypes have been screened for adventitious shoot formation in tissue culture. Best response in termis of multiple shoot induction was obtained from cotyledon proximal part with hypocotyl segmenet on Murashige and Skoog medium supplemented with 1 mg/l 6-Benzylaminopurine, 30 g/l sucrose and 8 g/l agar. After 3 weeks of culture, 90% of cotyledon proximal parts induced shoot. An average of 12.6 shoots per explants and a mean shoot length of 8 mm were obtained after multiplication stage. Shoot induction appeared to be strongly influenced by genotype and explant type. The percentage of shoot induction from cotyledon proximal parts ranged from 23.3% to 64.0% according to the genotype. Efficient rooting was achieved on half-solid MS medium containing 0.1 mg/l 1-Naphthaleneactic acid or without growth regulator. Acclimatised plantlets were transferred to greenhouse where they grew and flowered like seeded plantlets.
http://hdl.handle.net/2268/106032

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
gnamien cucurbits tropicultura dec 2010.pdfPublisher postprint403.35 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.