Reference : In-vivo studies on Haemaccel-fibronectin interaction in man.
Scientific journals : Article
Human health sciences : Anesthesia & intensive care
http://hdl.handle.net/2268/10597
In-vivo studies on Haemaccel-fibronectin interaction in man.
English
Damas, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Adam, Aurore [Centre Hospitalier Universitaire de Liège - CHU > > Salle Médecine A NDB >]
Buret, J. [> > > >]
Renard, Cécile [Centre Hospitalier Universitaire de Liège - CHU > > Rééducation de la parole >]
Lamy, Maurice mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Foidart, Jacqueline mailto [Centre Hospitalier Universitaire de Liège - CHU > > Médecine nucléaire >]
Mahieu, P. [> > > >]
1987
European Journal of Clinical Investigation
Blackwell Publishing
17
2
166-73
Yes (verified by ORBi)
International
0014-2972
1365-2362
Oxford
United Kingdom
[en] Chromatography, Affinity ; Chromatography, Gel ; Enzyme-Linked Immunosorbent Assay ; Fibronectins/metabolism ; Humans ; Polygeline/pharmacology ; Polymers/pharmacology ; Protein Binding
[en] An enzyme-linked immunoassay has been recently set up for direct measurement of the binding capacity of plasma fibronectin to gelatin. This binding capacity could be completely inhibited in vitro by an eight-fold excess of gelatin, of Haemaccel, but not of Geloplasma. On the contrary, the levels of immunoreactive fibronectin measured by laser nephelometry did not change, in presence of 10 to 1000 micrograms ml-1 of gelatin, of Haemaccel or of Geloplasma. When infused into normal volunteers, Haemaccel provoked a strong and immediate inhibition of the plasma fibronectin binding capacity to gelatin. This inhibition was dose-dependent and maximal after infusion of 500 ml of Haemaccel. Twenty-four hours after this infusion, there was a progressive recovery of the gelatin-binding capacity, which was almost completely achieved 96 h later. The formation of complexes between Haemaccel and fibronectin was demonstrated by gel filtration chromatography and by affinity chromatography. Immunoreactive plasma fibronectin levels remained unchanged up to 24 h after infusion of 500 ml of Haemaccel. A transient decline to 50% of its initial value then occurred the second day after the infusion. Therefore, a delay existed between the formation of fibronectin-Haemaccel complexes and their elimination from the bloodstream. This delay decreased when smaller volumes of Haemaccel were infused, which strongly suggests that plasma fibronectin is cleared by means of Haemaccel and does not seem to play a role of opsonin in these conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
http://hdl.handle.net/2268/10597

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