Article (Scientific journals)
Spleen-dependent turnover of CD11b peripheral blood B lymphocytes in bovine leukemia virus-infected sheep.
Florins, Arnaud-Francois; Gillet, Nicolas; Asquith, Becca et al.
2006In Journal of Virology, 80 (24), p. 11998-2008
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Keywords :
Animals; Antigens, CD11b/immunology; Apoptosis/immunology; B-Lymphocytes/immunology; Cell Proliferation; DNA Primers; Deltaretrovirus Infections/immunology; Fluoresceins; Homeostasis/immunology; Immunophenotyping; Kinetics; Leukemia Virus, Bovine/immunology; Sheep; Spleen/immunology; Splenectomy; Succinimides
Abstract :
[en] Lymphocyte homeostasis is determined by a critical balance between cell proliferation and death, an equilibrium which is deregulated in bovine leukemia virus (BLV)-infected sheep. We have previously shown that an excess of proliferation occurs in lymphoid tissues and that the peripheral blood population is prone to increased cell death. To further understand the mechanisms involved, we evaluated the physiological role of the spleen in this accelerated turnover. To this end, B lymphocytes were labeled in vivo using a fluorescent marker (carboxyfluorescein diacetate succinimidyl ester), and the cell kinetic parameters (proliferation and death rates) of animals before and after splenectomy were compared. We show that the enhanced cell death observed in BLV-infected sheep is abrogated after splenectomy, revealing a key role of the spleen in B-lymphocyte dynamics.
Disciplines :
Veterinary medicine & animal health
Oncology
Author, co-author :
Florins, Arnaud-Francois ;  Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc.
Gillet, Nicolas ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Asquith, Becca
Debacq, Christophe
Jean, Geneviève ;  Université de Liège - ULiège > Sciences agronomiques > Zootechnie
Schwartz-Cornil, Isabelle
Bonneau, Michel
Burny, Arsène ;  Université de Liège - ULiège > Agro Biotech Gembloux
Reichert, Michal
Kettmann, Richard ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Willems, Luc  ;  Université de Liège - ULiège > GIGA-Research
Language :
English
Title :
Spleen-dependent turnover of CD11b peripheral blood B lymphocytes in bovine leukemia virus-infected sheep.
Publication date :
2006
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
80
Issue :
24
Pages :
11998-2008
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 April 2009

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