Reference : Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimid...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (CFSE).
Asquith, Becca [Imperial College London > Department of Immunology > >]
Debacq, Christophe [> > > >]
Florins, Arnaud-Francois [Université de Liège - ULg > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc. >]
Gillet, Nicolas mailto [Université de Liège - ULg > Chimie et bio-industries > Biologie cell. et moléc. >]
Sanchez-Alcaraz, Teresa [> > > >]
Mosley, Angelina [> > > >]
Willems, Luc mailto [Université de Liège - ULg > > GIGA-Research >]
Proceedings of the Royal Society B : Biological Sciences
Royal Society
Yes (verified by ORBi)
United Kingdom
[en] Animals ; B-Lymphocytes/metabolism/physiology ; Cell Division ; Flow Cytometry ; Fluoresceins/metabolism ; Fluorescent Dyes/metabolism ; Kinetics ; Mathematics ; Sheep ; Succinimides/metabolism
[en] The cytoplasmic dye carboxyfluorescein diacetate succinimidyl ester (CFSE) is used to quantify cell kinetics. It is particularly important in studies of lymphocyte homeostasis where its labelling of cells irrespective of their stage in the cell cycle makes it preferable to deuterated glucose and BrdU, which only label dividing cells and thus produce unrepresentative results. In the past, experiments have been limited by the need to obtain a clear separation of CFSE peaks forcing scientists to adopt a strategy of in vitro labelling of cells followed by their injection into the host. Here we develop a framework for analysis of in vivo CFSE labelling data. This enables us to estimate the rate of proliferation and death of lymphocytes in situ, and thus represents a considerable advance over current procedures. We illustrate this approach using in vivo CFSE labelling of B lymphocytes in sheep.

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