Reference : Myeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/105492
Myeloperoxidase in Equine Semen: Concentration and Localization during Freezing Processing
English
Ponthier, Jérôme mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Desvals, Maud [ > > ]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
de la Rebière de Pouyade, Geoffroy mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Spalart, Marc [ > > ]
Palmer, Eric [ > > ]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Deleuze, Stefan mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Obstét. et path. de la reprod. des anim. de comp. et équidés >]
Jan-2012
Journal of Equine Veterinary Science
Elsevier
32
32-37
Yes (verified by ORBi)
International
0737-0806
New York
NY
[en] Stallion ; Semen ; Cryopreservation ; Myeloperoxidase ; Non-sperm cells ; Freezing
[en] Myeloperoxidase (MPO) is a pro-oxidant enzyme contained in and released by neutro- phils during degranulation or after lysis. Post-thaw semen contains MPO, and concen- tration of this enzyme is associated with decreased motility. The aim of this study was to determine kinetics of MPO concentration during freezing, its origin, and its impact on frozenethawed semen. Forty ejaculates were used. Semen was frozen using the classical freezing procedure. MPO concentrations were assayed in fresh semen, after centrifuga- tion, and after cooling down to 4 C. Post-thaw MPO assay results and spermogram characteristics were determined. MPO immunocytochemistry was performed on 4 different ejaculates at each step of freezing procedure. MPO concentration increased after cooling down to 4 C and thawing compared with fresh semen. As temperature decreased, MPO was higher or tended to be higher in post-thaw poor quality samples. Nonsperm cells showed various degrees of MPO immunostaining and were observed as epithelial cells with nuclear pyknosis and keratinization. MPO immunostaining increased in medium and decreased in nonsperm cells during freezing. Our study shows that MPO concentration in equine semen increases when temperature decreases. We hypothesize that nonsperm cells present in fresh semen could release MPO.
Pathologie de la reproduction des Equidés - CVU - FMV - ULg
Clinique Equine - CVU - FMV - ULg ; Pathologie de la reproduction des Equidés et Carnivores domestiques - CVU - FMV - ULg
SPERMPO
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/105492

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