Article (Scientific journals)
Early activation of cardiac and renal endothelin systems in experimental heart failure
Motte, S.; van Beneden, R.; Mottet, J. et al.
2003In American Journal of Physiology - Heart and Circulatory Physiology, 285 (6), p. 2482-H2491
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Keywords :
gene expression; receptors; biopsy
Abstract :
[en] We investigated the time course of the expression of cardiac and renal endothelin systems in tachycardia-induced heart failure in dogs. Eleven beagles underwent rapid pacing at a progressively increased rate over a period of 5 wk, with a weekly clinical examination, echocardiography, measurement of circulating and urinary endothelin-1 (ET-1), and myocardial and renal tissue biopsies. Real-time quantitative PCR was used for determinations of tissue prepro-ET-1 (ppET-1), ET-1-converting enzyme (ECE-1), and ETA and ETB receptor mRNA. Cardiac and renal tissue ET-1 contents were evaluated by immunostaining and measured by radioimmunoassay at autopsy. Rapid pacing caused a progressive increase in end-systolic and end-diastolic ventricular volumes (P<0.05) from week 2 together with a decrease in ejection fraction and in mean velocity of circumferential shortening (P<0.05) from week 1. These changes were tightly correlated to myocardial ppET-1 and renal ETA receptor mRNA and less so to myocardial ECE-1 mRNA, and they occurred before any increase in plasma and urinary ET-1 (P<0.05 from week 4) and clinical signs of heart failure. Renal ppET-1 did not change. Both cardiac and renal ET-1 peptide contents were increased at autopsy. We conclude that tachycardia-induced heart failure in dogs is characterized by an early activation of the cardiac and renal tissue endothelin systems, which occurs before any changes in circulating and urinary ET-1 and is closely related to altered ventricular function.
Disciplines :
Cardiovascular & respiratory systems
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Motte, S.
van Beneden, R.
Mottet, J.
Rondelet, B.
Mathieu, M.
Havaux, X.
Lause, P.
Clercx, Cécile  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Ketelslegers, J. M.
Naeije, R.
Mc Entee, Kathleen ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Sémiologie et clinique médicale des petits animaux
Language :
English
Title :
Early activation of cardiac and renal endothelin systems in experimental heart failure
Publication date :
01 December 2003
Journal title :
American Journal of Physiology - Heart and Circulatory Physiology
ISSN :
0363-6135
eISSN :
1522-1539
Publisher :
Amer Physiological Soc, Bethesda, United States - Maryland
Volume :
285
Issue :
6
Pages :
H2482-H2491
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 December 2011

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