ORBi: Motte S. - Early activation of cardiac and renal endothelin systems in experimental heart failure

Reference : Early activation of cardiac and renal endothelin systems in experimental heart failure


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Cardiovascular & respiratory systems Life sciences : Anatomy (cytology, histology, embryology...) & physiology
	To cite this reference:	http://hdl.handle.net/2268/104884

Title :	Early activation of cardiac and renal endothelin systems in experimental heart failure
Language :	English
Author, co-author :	Motte, S. [> > > >]
	van Beneden, R. [> > > >]
	Mottet, J. [> > > >]
	Rondelet, B. [> > > >]
	Mathieu, M. [> > > >]
	Havaux, X. [> > > >]
	Lause, P. [> > > >]
	Clercx, Cécile [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux >]
	Ketelslegers, J. M. [> > > >]
	Naeije, R. [> > > >]
	Mc Entee, Kathleen [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Sémiologie et clinique médicale des petits animaux >]
Publication date :	1-Dec-2003
Journal title :	American Journal of Physiology - Heart and Circulatory Physiology
Publisher :	Amer Physiological Soc
Volume :	285
Issue/season :	6
Pages :	H2482-H2491
Audience :	International
ISSN :	0363-6135
City :	Bethesda
Keywords :	[en] gene expression ; receptors ; biopsy
Abstract :	[en] We investigated the time course of the expression of cardiac and renal endothelin systems in tachycardia-induced heart failure in dogs. Eleven beagles underwent rapid pacing at a progressively increased rate over a period of 5 wk, with a weekly clinical examination, echocardiography, measurement of circulating and urinary endothelin-1 (ET-1), and myocardial and renal tissue biopsies. Real-time quantitative PCR was used for determinations of tissue prepro-ET-1 (ppET-1), ET-1-converting enzyme (ECE-1), and ETA and ETB receptor mRNA. Cardiac and renal tissue ET-1 contents were evaluated by immunostaining and measured by radioimmunoassay at autopsy. Rapid pacing caused a progressive increase in end-systolic and end-diastolic ventricular volumes (P<0.05) from week 2 together with a decrease in ejection fraction and in mean velocity of circumferential shortening (P<0.05) from week 1. These changes were tightly correlated to myocardial ppET-1 and renal ETA receptor mRNA and less so to myocardial ECE-1 mRNA, and they occurred before any increase in plasma and urinary ET-1 (P<0.05 from week 4) and clinical signs of heart failure. Renal ppET-1 did not change. Both cardiac and renal ET-1 peptide contents were increased at autopsy. We conclude that tachycardia-induced heart failure in dogs is characterized by an early activation of the cardiac and renal tissue endothelin systems, which occurs before any changes in circulating and urinary ET-1 and is closely related to altered ventricular function.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/104884

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