Reference : Upregulation of MMPs by soluble E-cadherin in human lung tumor cells
Scientific journals : Article
Human health sciences : Oncology
http://hdl.handle.net/2268/10440
Upregulation of MMPs by soluble E-cadherin in human lung tumor cells
English
Nawrocki-Raby, B. [> > > >]
Gilles, Christine mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Polette, M. [> > > >]
Bruynfeel, E. [> > > >]
Laronze, J. Y. [> > > >]
Bonnet, N. [> > > >]
Foidart, Jean-Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique >]
Mareel, M. [> > > >]
Birembaut, P. [> > > >]
20-Jul-2003
International Journal of Cancer = Journal International du Cancer
Wiley Liss, Inc.
105
6
790-795
Yes (verified by ORBi)
International
0020-7136
1097-0215
New York
NY
[en] cancer progression ; soluble E-cadherin ; MMPs ; cell invasion
[en] Loss of E-cadherin/catenin mediated cell-cell adhesion and overexpression of matrix metalloproteinases (MMPs) are largely involved in tumor invasion. It has been recently shown that high levels of a soluble 80 kDa fragment of E-cadherin, resulting from a cleavage by IVIMPs, are found in serum and in urine from cancer patients. Additionally, this soluble E-cadherin (sE-CAD) promotes cell invasion into chick heart and into collagen type I gels. The aim of our study was to examine the mechanism of sE-CAD-induced cell invasion. Since MMPs play a crucial role in invasion, we looked for induction of MMPs by sE-CAD in noninvasive human lung tumor cells 16HBE. An induction of MMP-2, MMP-9 and MTI-MMP expression was observed both at the mRNA and at the protein level in the presence of sE-CAD (in conditioned medium form or in E-cadherin HAV peptide form). No induction of MMP-I, -3 and -7 or variation of the levels of their inhibitors, TIMP-I and TIMP-2, were detected. The biologic relevance of the sE-CAD-induced MMP upregulation was tested by demonstrating that sE-CAD promotes in vitro cell invasion in a modified Boyden chamber assay. These data provide new insight into mechanisms of tumor invasion by ectodomain shedding of the cell-cell adhesion molecule E-cadherin. (C) 2003 Wiley-Liss, Inc.
http://hdl.handle.net/2268/10440

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