Reference : Development of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantifi...
Scientific journals : Article
Life sciences : Agriculture & agronomy
Life sciences : Biotechnology
http://hdl.handle.net/2268/103960
Development of a Quick Quantitative Real-Time PCR for the In Vivo Detection and Quantification of Peach latent mosaic viroid
English
Parisi, Olivier mailto [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Lepoivre, Philippe mailto [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Jijakli, Haissam mailto [Université de Liège - ULg > Sciences agronomiques > Phytopathologie >]
Feb-2011
Plant Disease
American Phytopathological Society
95
2
137-142
Yes (verified by ORBi)
International
0191-2917
[en] viroid ; real-time PCR ; quantitative PCR
[en] Viroids are plant pathogens infecting a broad range of herbaceous and
tree crops. Among them, the Peach latent mosaic viroid (PLMVd)
infects mainly peach trees, causing a loss of production with no
curative options. Detecting this viroid is thus important for certification
procedures aiming to avoid the release of infected material into
orchards. Presented here is a complete detection method based on
reverse transcription (RT) followed by a quantitative real-time
polymerase chain reaction (PCR). New primers were selected and
optimal reaction conditions determined for routine application of the
method. The technique is 105 times more sensitive than the endpoint
RT-PCR used for PLMVd detection, and permits earlier detection of
PLMVd in infected plants. The quick, low-cost extraction procedure
used and the quality of the results obtained make this method suitable
for routine testing.
Researchers ; Professionals ; Students ; Others
http://hdl.handle.net/2268/103960

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Parisi et al 2011.pdfPublisher postprint815.95 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.