ORBi: Tocquin Pierre - Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N:P ratio on vegetative growth

Reference : Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...)
	To cite this reference:	http://hdl.handle.net/2268/103905

Title :	Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N:P ratio on vegetative growth
Language :	English
Author, co-author :	Tocquin, Pierre [Université de Liège - ULg > Département des sciences de la vie > labo de Bioénergétique > >]
	Fratamico, Anthony [Université de Liège - ULg > > Labo de Bioénergétique >]
	Franck, Fabrice [Université de Liège - ULg > > Labo de Bioénergétique >]
Publication date :	2012
Journal title :	Journal of Applied Phycology
Publisher :	Springer
Volume :	24
Issue/season :	3
Pages :	365-373
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0921-8971
e-ISSN :	1573-5176
City :	Dordrecht
Country :	Netherlands
Keywords :	[en] Haematococcus pluvialis ; Culture media optimization ; Phosphate supply ; Nutrient induced fluorescence transients
Abstract :	[en] Haematococcus pluvialis is the current better source of natural astaxanthin, a high-value carotenoid. Traditionally, the production process of astaxanthin by this algae is achieved by a two-stage system: during the first stage, vegetative “green” cells are produced and then converted, in the second stage, into cysts that accumulate astaxanthin. In this work, a medium screening strategy based on the mixing of a 3-component hydroponic fertilizer was applied to identify a new formulation optimized for the vegetative stage. A maximal and high cell density of 2 x 106 cells mL−1 was obtained in a medium containing a high level of phosphate relative to nitrate, resulting in a N:P ratio much lower than commonly used media for H. pluvialis. In this medium, cells remained at the vegetative and motile stage during a prolonged period of time. Both high cell density culture and motile stage persistence was proved to be related to the N:P feature of this medium. We conclude that the macrozoid stage of H. pluvialis is favored under high-P and low-N supply and that low-cost hydroponic fertilizers can be successfully used for achieving high density cultures of vegetative cells of H. pluvialis.
Funders :	Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Name of the research project :	BIOVAMAT
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/103905
DOI :	10.1007/s10811-011-9771-3

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Open access	2011-JAPH-2.pdf	Author postprint color version	Author postprint	1.69 MB	View/Open
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