Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers (SSR)

[en] Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and more and more it arouse interest of scientific community given its numerous biological activities. However, information about its genetic resources and characterization using reliable molecular markers are still scarce. In the present study, we report the development of 4 new polymorphic SSR markers. They have been used in addition to 11 SSRs previously published to investigate molecular diversity of 33 Punica granatum ecotypes. Based on the multi-locus profiles, twenty-two distinctive genotypes were identified. Globally, quite low genetic diversity has been revealed, as measured by allele richness (2.83 per locus) and heterozygosity (He = 0.245; Ho = 0.243), reflecting the narrow genetic background of the plant material. Four synonymous groups could be detected involving 15 accessions. Results of ordination and cluster analysis suggested that almost all the Tunisian cultivars share similar genetic background, and are likely derived from a small number of introductions in ancient times. Results issued from this study provide essential information to project a previous termpomegranatenext term core-collection without plant material duplication and for sustainable management of previous termpomegranatenext term landraces at national and international level. Furthermore, these SSR markers are powerful tool for marker assisted selection (MAS) program and for QTL studies.

Disciplines :

Agriculture & agronomy

Author, co-author :

Hasnaoui, Nejib ; Institut Supérieur Agronomique, Chott Meriam 4042, Sousse, Tunisie > U.R. Agrobiodiversité

Buonamici, Anna; Instituto di Genetica Vegetale (CNR-IGV), 50019 Sesto Fiorentino, Florence, Italy

Sebastiani, Federico; Genexpress, Università di Firenze, 50019 Sesto Fiorentino, Florence, Italy > Dpt. Biotechnologie Agrarie

Mars, Messaoud; Institut Supérieur Agronomique, Chott Meriam 4042, Sousse, Tunisia > U.R. Agrobiodiversité

Zhand, Dapeng; Plant Sciences Institute, Beltsville Agricultural Research Center, USDA/ARS, MD 20705, USA > Sustainable Perennial Crops Laboratory

Vendramin, Giovanni; Instituto di Genetica Vegetale (CNR-IGV), 50019 Sesto Fiorentino, Florence, Italy

Language :

English

Title :

Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers (SSR)

Publication date :

2012

Journal title :

Gene

ISSN :

0378-1119

eISSN :

1879-0038

Publisher :

Elsevier Science, Amsterdam, Netherlands

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://dx.doi.org/10.1016/j.gene.2011.11.012

Available on ORBi :

since 23 November 2011

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