Article (Scientific journals)
Pregnancy-associated glycoprotein, chymosin and pepsinogen immunoreactivity of proteins extracted from fetal gastric tissue in bovine species.
Bella, Amina; Melo de Sousa, Noelita; Dehimi, M. L. et al.
2012In Research in Veterinary Science, 92 (3), p. 378-386
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Keywords :
Stomach; foetus; pregnancy-associated glycoprotein; pepsinogen; chymosin; immunoreactivity
Abstract :
[en] The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical biochemical protocols were analyzed by proteolytic method, by PAG-RIA and by Western blot with the use of antisera raised against both pepsinogens and PAG. A strong reaction of proteins extracted from the fundic mucosa collected at the beginning of pregnancy was revealed with both anti-bPAG-I and anti-bPAG-II antisera, suggesting the expression of pepsinogen F in bovine species. Concerning pyloric mucosa, the analysis by Western blot highlighted a very strong immunoreaction with the anti-bovine chymosin serum. Amino-terminal sequencing allowed to identify bovine fetuin and albumin in fundic extracts, chymosin in the pyloric mucosa extracts, as well as some unknown proteins in both mucosa. Despite no N-terminal microsequence corresponding to the hypothetical pepsinogen F could be identified, it cannot be excluded that an existing bovine pepsinogen F-like molecule could be degraded during the purification procedure or that co-purified proteins could be responsible for masking its N-terminal microsequence.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Bella, Amina ;  Université de Liège - ULiège > Doct. sc. vété. (Bologne)
Melo de Sousa, Noelita ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Dehimi, M. L.
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Pregnancy-associated glycoprotein, chymosin and pepsinogen immunoreactivity of proteins extracted from fetal gastric tissue in bovine species.
Publication date :
2012
Journal title :
Research in Veterinary Science
ISSN :
0034-5288
eISSN :
1532-2661
Publisher :
British Veterinary Association, London, United Kingdom
Volume :
92
Issue :
3
Pages :
378-386
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 November 2011

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