ORBi: Bella Amina - Development of a new radioimmunoassay by using antiserum against pregnancy-associated glycoprotein II : concentrations in holstein dairy cattle .

Reference : Development of a new radioimmunoassay by using antiserum against pregnancy-associated gl...


	Document type :	Scientific congresses and symposiums : Paper published in a book
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/103763

Title :	Development of a new radioimmunoassay by using antiserum against pregnancy-associated glycoprotein II : concentrations in holstein dairy cattle .
Language :	English
Author, co-author :	Bella, Amina [Université de Liège - ULg > > > Doct. sc. vété. (Bologne)]
	Melo de Sousa, Noelita [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
	Lopez-Gatius, F. [ > > ]
	Garcia-Ispierto, I. [ > > ]
	Schoofs, L. [ > > ]
	Chahiba, Najia [Université de Liège - ULg > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP) >]
	Beckers, Jean-François [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Publication date :	7-Sep-2011
Main document title :	Proceedings of the 6th European Congress of Bovine Health and Management (ECBHM)
Pages :	63
Peer reviewed :	No
On invitation :	No
Audience :	International
ISBN :	9789079892020
Country :	Belgium
Event name :	6th European Congress of Bovine Health and Management (ECBHM)
Event date :	7-9 septembre 2011
Keywords :	[en] Pregnancy-associated glycoprotein ; cattle ; radioimmunoassay
Abstract :	[en] Bovine pregnancy-associated glycoproteins (boPAG) can be classified into two main subfamilies: the boPAG–1 and the boPAG–2 groups. RIA systems allow quantifying boPAG-1 molecules in pregnant cattle. Concerning boPAG-2, as no radioimmunoassay was developed, its quantification in peripheral blood of pregnant cows remains to be investigated. The present work aimed to develop a new heterologous RIA allowing the measurement of boPAG-2 concentrations in bovine species. A total of 77 Holstein dairy cows were used for this study. They were bled at Days 45, 90, 120, 150, 180, 210 after AI, at parturition and at Day 30 postpartum. Polyclonal antiserum against boPAG-2 (AS438) was raised in New Zealand rabbits. Due to the instability of boPAG-2 molecule, we used boPAG-1 (67 kDa) as standard (dilutions ranging from 100 to 0.8 ng/mL) and for iodination with 125-I isotope. The optimal dilution for primary AS438 was 1:1,1500. Mean concentrations of boPAG-2 increased continuously from Day 45 of pregnancy (0.78 ± 0.07 ng/mL) reaching a peak at Day 210 (32.78 ± 3.02 ng/mL). Thereafter, they decreased until parturition (7.73 ± 0.59). Lower PAG concentrations were observed at Day 30 postpartum (3.62 ± 0.31 ng/mL). In conclusion, boPAG-2 profile differed from boPAG-1. In general, concentrations of boPAG-2 reached lower values, with no dramatic increase being observed at peripartum period. Due to the lower postpartum concentrations, it is expected that this new RIA can be available for pregnancy diagnosis of cows inseminated in early postpartum, minimizing the risk of false positive concentrations due to the previous pregnancy.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/103763

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	File	Commentary	Version	Size	Access
Restricted access	BellaA_6ECBHM_2011.pdf		Publisher postprint	195.52 kB	Request copy
Restricted access	BeckersJF_ECBHM_bPAGII_2011.pdf		Publisher postprint	388.58 kB	Request copy

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