Reference : Mitochondrial DNA haplogroups and serum levels of proteolytic enzymes in patients wit...
Scientific journals : Article
Human health sciences : Rheumatology
http://hdl.handle.net/2268/103480
Mitochondrial DNA haplogroups and serum levels of proteolytic enzymes in patients with osteoarthritis.
English
Rego-Perez, I. [> > > >]
Fernandez-Moreno, M. [> > > >]
Deberg, Michelle mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)]
Pertega, S. [> > > >]
Fernandez-Lopez, C. [> > > >]
Oreiro, N. [> > > >]
Henrotin, Yves mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)]
Blanco, F. J. [> > > >]
2011
Annals of the Rheumatic Diseases
BMJ Group
70
4
646-52
Yes (verified by ORBi)
International
0003-4967
1468-2060
London
United Kingdom
[en] Aged ; Aged, 80 and over ; Biological Markers/blood ; Case-Control Studies ; DNA, Mitochondrial/genetics ; Female ; Genetic Predisposition to Disease ; Haplotypes ; Humans ; Male ; Matrix Metalloproteinase 13/blood ; Matrix Metalloproteinase 3/blood ; Middle Aged ; Osteoarthritis, Hip/enzymology/genetics ; Osteoarthritis, Knee/enzymology/genetics ; Peptide Hydrolases/blood
[en] OBJECTIVE: To analyse the influence of mitochondrial DNA haplogroups, as well as the radiographic grade, on serum levels of proteolytic enzymes in patients with osteoarthritis (OA). METHODS: Serum levels of metalloproteinase-1 (MMP-1), MMP-3, MMP-13, myeloperoxidase and cathepsin K were analysed in 73 patients with OA and 77 healthy controls carrying the haplogroups J, U and H, by ELISA. Knee and hip radiographs were classified according to Kellgren and Lawrence (K/L) scoring from grade 0 to grade IV. Non-parametric and multiple regression analyses were performed to test the effects of clinical variables, including gender, age, smoking status, diagnosis, haplogroups and radiological K/L grade on serum levels of these enzymes. RESULTS: A significant influence of the haplogroups on the serum levels of MMP-3 and MMP-13 was detected (p=0.027 and p=0.035, respectively). Patients with OA with haplogroup H showed higher serum levels of MMP-3 than healthy controls. Serum levels of MMP-13 were significantly higher in patients with OA (p<0.001), and carriers of the haplogroup J showed lower levels than H carriers. Besides, levels of MMP-13 were proportionally higher in radiological groups B (K/L grade II and III) and C (K/L grade IV) than in group A (K/L grade 0 and I) (p=0.005). CONCLUSIONS: This study shows that haplogroups have a significant influence on serum levels of MMP-3 and MMP-13. The influence of the haplogroups on serum levels of MMP-3 is clearly dependent on the diagnosis, whereas the influence of the haplogroups on serum levels of MMP-13 is independent of diagnosis.
http://hdl.handle.net/2268/103480
10.1136/ard.2010.133637

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