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| Reference : Expression of receptors for insulin-like growth factor-I and transforming growth factor-... |
| Scientific journals : Article | |||
| Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics) | |||
| http://hdl.handle.net/2268/103317 | |||
| Expression of receptors for insulin-like growth factor-I and transforming growth factor-beta in human follicles. | |
| English | |
| Qu, Jian Ping [ > > ] | |
| GODIN, Pierre-Arnaud [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique >] | |
NISOLLE, Michelle  [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >] | |
| Donnez, Jacques [ > > ] | |
| 2000 | |
| Molecular Human Reproduction | |
| Oxford University Press | |
| 6 | |
| 2 | |
| 137-45 | |
| International | |
| 1360-9947 | |
| 1460-2407 | |
| Oxford | |
| United Kingdom | |
| [en] cryopreservation ; follicle ; insulin-like growth factor receptor ; transforming growth factor-β | |
| [en] The in-vitro growth of immature oocytes in early follicles from cryopreserved human ovarian tissues is a new concept in in-vitro fertilization programmes for the treatment of infertile and cancer patients. To better understand the regulatory mechanism of follicular development, immunohistochemistry was used to study the expression of insulin-like growth factor (IGF) type I receptor (IGF-IR) and transforming growth factor-β (TGFβ) type I (TβR-I) and type II (TβR-II) receptors in fresh and frozen ovarian tissues from 14 women. Immunoreactivities for IGF-IR and TβR-I were present simultaneously in the oocytes of primordial, pre-antral and antral follicles. Staining for both IGF-IR and TβR-I was also observed in granulosa cells of primordial, pre-antral and antral follicles. IGF-IR and TβR-I also stained in thecal cells of pre-antral and antral follicles. Stromal cells in surrounding ovarian tissue expressed IGF-IR and TβR-I at various follicular stages. Unlike TβR-I, TβR-II was expressed only in the oocytes of primordial and primary follicles, and with weak staining intensity in thecal cells. No significant staining for TβR-II was found in oocytes and granulosa cells of antral follicles. There was no difference in staining patterns for IGF-IR, TβR-I and TβR-II between fresh and frozen ovarian tissues, indicating that cryopreservation might not significantly alter the immunoreactivities of these receptors in frozen ovarian tissue. The results suggest that IGF-I and TGFβ may participate in the regulation of follicular growth by binding to their receptors through an autocrine or paracrine mechanism. IGF-I and TGFβ may be useful in regulating the in-vitro or in-vivo maturation of oocytes not only in later follicles but also very early follicles, from cryopreserved ovarian tissues for clinical use in the future. | |
| http://hdl.handle.net/2268/103317 | |
| 10.1093/molehr/6.2.137 |
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