ORBi: Van Langendonckt Anne - Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.

Reference : Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the cult...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
	To cite this reference:	http://hdl.handle.net/2268/103304

Title :	Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.
Language :	English
Author, co-author :	Van Langendonckt, Anne [ > > ]
	Demylle, Dominique [ > > ]
	Wyns, Christine [ > > ]
	NISOLLE, Michelle [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
	Donnez, Jacques [ > > ]
Publication date :	2001
Journal title :	Fertility and Sterility
Publisher :	Elsevier
Volume :	76
Issue/season :	5
Pages :	1023-31
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0015-0282
e-ISSN :	1556-5653
City :	New York
Country :	NY
Keywords :	[en] Human embryo culture ; sequential culture medium ; IVF ; blastocyst ; implantation rate
Abstract :	[en] Objective: To compare two commercially available sequential media, G1.2/G2.2 and Sydney IVF cleavage/blastocyst media, as supports for human embryo culture. Design: Prospective randomized study. Setting: University-based IVF clinic. Patient(s): Two hundred forty-nine patients undergoing IVF treatment for the first or second time, randomly allocated at the time of oocyte retrieval, to either culture in G1.2/G2.2 or Sydney IVF media. Intervention(s): Oocyte recovery, IVF or intracytoplasmic sperm injection, embryo culture, transfer on day 3 or day 5/6. Main Outcome Measure(s): Developmental stage on day 3, blastocyst rate, pregnancy outcome as assessed by βhCG positive test, implantation rates, and ongoing pregnancies. Result(s): Embryos cultured in G1.2/G2.2 media displayed a faster kinetics of cleavage, compaction, blastulation, and hatching, but a lower day 3 embryo quality than those grown in Sydney IVF media. For patients with at least five embryos, G1.2/G2.2 media yielded higher implantation rates (26.2%) in our day 3 embryo transfer program when compared to Sydney IVF medium (15.5%), whereas similar implantation rates were obtained for day 5/6 embryo transfer for both media (43.1% and 36.1%, respectively). Conclusion(s): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program.
Permalink :	http://hdl.handle.net/2268/103304
DOI :	10.1016/S0015-0282(01)02854-0

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