|Reference : Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the cult...|
|Scientific journals : Article|
|Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)|
|Comparison of G1.2/G2.2 and Sydney IVF cleavage/blastocyst sequential media for the culture of human embryos: a prospective, randomized, comparative study.|
|Van Langendonckt, Anne [ > > ]|
|Demylle, Dominique [ > > ]|
|Wyns, Christine [ > > ]|
|NISOLLE, Michelle [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]|
|Donnez, Jacques [ > > ]|
|Fertility and Sterility|
|Yes (verified by ORBi)|
|[en] Human embryo culture ; sequential culture medium ; IVF ; blastocyst ; implantation rate|
|[en] Objective: To compare two commercially available sequential media, G1.2/G2.2 and Sydney IVF cleavage/blastocyst media, as supports for human embryo culture.
Design: Prospective randomized study.
Setting: University-based IVF clinic.
Patient(s): Two hundred forty-nine patients undergoing IVF treatment for the first or second time, randomly allocated at the time of oocyte retrieval, to either culture in G1.2/G2.2 or Sydney IVF media.
Intervention(s): Oocyte recovery, IVF or intracytoplasmic sperm injection, embryo culture, transfer on day 3 or day 5/6.
Main Outcome Measure(s): Developmental stage on day 3, blastocyst rate, pregnancy outcome as assessed by βhCG positive test, implantation rates, and ongoing pregnancies.
Result(s): Embryos cultured in G1.2/G2.2 media displayed a faster kinetics of cleavage, compaction, blastulation, and hatching, but a lower day 3 embryo quality than those grown in Sydney IVF media. For patients with at least five embryos, G1.2/G2.2 media yielded higher implantation rates (26.2%) in our day 3 embryo transfer program when compared to Sydney IVF medium (15.5%), whereas similar implantation rates were obtained for day 5/6 embryo transfer for both media (43.1% and 36.1%, respectively).
Conclusion(s): In our day 3 embryo transfer program, G1.2/G2.2 media were superior to Sydney IVF media, whereas both media yielded similar outcomes in our blastocyst transfer program.
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