Reference : Study on the susceptibility of the bovine milk fat globule membrane proteins to enzym...
Scientific journals : Article
Life sciences : Food science
http://hdl.handle.net/2268/103267
Study on the susceptibility of the bovine milk fat globule membrane proteins to enzymatic hydrolysis and organization of some of the proteins
English
Vanderghem, Caroline mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
Francis, Frédéric mailto [Université de Liège - ULg > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Danthine, Sabine mailto [Université de Liège - ULg > Chimie et bio-industries > Laboratoire des Sciences des aliments et formulation >]
Deroanne, Claude [ > > ]
Paquot, Michel mailto [Université de Liège - ULg > Chimie et bio-industries > Chimie biologique industrielle >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Blecker, Christophe mailto [Université de Liège - ULg > Chimie et bio-industries > Laboratoire des Sciences des aliments et formulation >]
May-2011
International Dairy Journal
Elsevier Science
21
5
312-318
Yes (verified by ORBi)
International
0958-6946
[en] ACID-BINDING PROTEIN ; MAMMARY-GLAND; PROTEOLYTIC DIGESTION; ; ELECTRON-MICROSCOPY
[en] Isolated milk fat globules were subjected to enzyme hydrolysis by a specific protease (trypsin) and a nonspecific protease (pronase E) to study the asymmetric arrangement of milk fat globule membrane (MFGM) proteins. The remaining proteins on the globules after proteolysis were resolved by two-dimensional gel electrophoresis and identified by mass spectrometry. By this proteomic approach, the results confirmed different susceptibility of the MFGM proteins to proteolysis by enzymes. Butyrophilin and adipophilin were completely digested by trypsin and by pronase E, whereas lactadherin and xanthine dehydrogenase/oxidase were almost resistant to hydrolysis by trypsin and partially attacked by pronase E. Based on our results and recent bibliographic data, an up-dated model of the organization of some MFGM proteins is proposed and discussed. (c) 2011 Elsevier Ltd. All rights reserved.
Researchers
http://hdl.handle.net/2268/103267
10.1016/j.idairyj.2010.12.006

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