Reference : Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features
Scientific journals : Article
Life sciences : Genetics & genetic processes
Life sciences : Veterinary medicine & animal health
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/103138
Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features
English
Palmeira, Leonor [Université de Liège - ULg > > Immunologie et vaccinologie >]
Machiels, Bénédicte mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Lété, Céline mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
2011
Virology Journal
8
1
406
Yes
International
[en] Background

Bovine herpesvirus 4 (BoHV-4) is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome (BAC), the lack of a complete genome sequence for this strain limits its experimental use.
Methods

In this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach.
Results

The long unique coding region (LUR) consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units (prDNA). As previously suggested, we showed that the prDNA unit located at the left prDNA-LUR junction (prDNA-G) differs from the other prDNA units (prDNA-inner). Namely, the prDNA-G unit lacks the conserved pac-2 cleavage and packaging signal in its right terminal region. Based on the mechanisms of cleavage and packaging of herpesvirus genomes, this feature implies that only genomes bearing left and right end prDNA units are encapsulated into virions.
Conclusions

In this study, we have determined the complete genome sequence of the BAC-cloned BoHV-4 V.test strain and identified genome organization features that could be important in other herpesviruses.
http://hdl.handle.net/2268/103138
10.1186/1743-422X-8-406
http://www.virologyj.com/content/8/1/406/

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