Reference : Assessment of reactive oxygen species production in cultured equine skeletal myoblast...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/102882
Assessment of reactive oxygen species production in cultured equine skeletal myoblasts in response to conditions of anoxia followed by reoxygenation with or without exposure to peroxidases.
English
Ceusters, Justine mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
de la Rebière de Pouyade, Geoffroy mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Votion, Dominique mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Deby-Dupont, Ginette mailto [> >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
2012
American Journal of Veterinary Research
American Veterinary Medical Association
73
3
426-434
Yes (verified by ORBi)
International
0002-9645
Schaumburg
IL
[en] equine myoblasts ; anoxia-reoxygenation ; reactive oxygen species
[en] Objective—To culture equine myoblasts from muscle microbiopsy specimens, examine
myoblast production of reactive oxygen species (ROS) in conditions of anoxia followed by
reoxygenation, and assess the effects of horseradish peroxidase (HRP) and myeloperoxidase
(MPO) on ROS production.
Animals—5 healthy horses (5 to 15 years old).
Procedures—Equine skeletal myoblast cultures were derived from 1 or 2 microbiopsy
specimens obtained from a triceps brachii muscle of each horse. Cultured myoblasts were
exposed to conditions of anoxia followed by reoxygenation or to conditions of normoxia
(control cells). Cell production of ROS in the presence or absence of HRP or MPO was
assessed by use of a gas chromatography method, after which cells were treated with a
3,3′-diaminobenzidine chromogen solution to detect peroxidase binding.
Results—Equine skeletal myoblasts were successfully cultured from microbiopsy specimens.
In response to anoxia and reoxygenation, ROS production of myoblasts increased by
71%, compared with that of control cells. When experiments were performed in the presence of HRP or MPO, ROS production in myoblasts exposed to anoxia and reoxygenation
was increased by 228% and 183%, respectively, compared with findings for control cells.
Chromogen reaction revealed a close adherence of peroxidases to cells, even after several
washes.
Conclusions and Clinical Relevance—Results indicated that equine skeletal myoblast
cultures can be generated from muscle microbiopsy specimens. Anoxia-reoxygenation–
treated myoblasts produced ROS, and production was enhanced in the presence of peroxidases.
This experimental model could be used to study the damaging effect of exercise on
muscles in athletic horses.
Centre de l’Oxygène, Recherche et Développement - CORD
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/102882

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
10-04-0126r.pdfAuthor preprint308.11 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.