[en] Glycoprotein B (gB) is a conserved, essential component of gammaherpes virions and so potentially vulnerable to neutralization. However, few good gB-specific neutralizing antibodies have been identified. Here, we show that murid herpesvirus 4 is strongly neutralized by mAbs that recognize an epitope close to one of the gB fusion loops. Antibody binding did not stop gB interacting with its cellular ligands or initiating its fusion-associated conformation change, but did stop gB resolving stably to its post-fusion form, and so blocked membrane fusion to leave virions stranded in late endosomes. The conservation of gB makes this mechanism a possible general route to gammaherpesvirus neutralization.
Disciplines :
Microbiology
Author, co-author :
Glauser, Daniel L
Kratz, Anne*-Sophie
Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie
Stevenson, Philip G
Language :
English
Title :
A mechanistic basis for potent, glycoprotein B-directed gammaherpesvirus neutralization.
Publication date :
2011
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Society for General Microbiology, London, United Kingdom
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