Effects of glucocorticoids on the respiratory burst of Chlamydia-primed THP-1 cells.

Anti-Inflammatory Agents/pharmacology; Cell Differentiation; Cell Line; Chlamydophila pneumoniae/metabolism/pathogenicity/physiology; Electron Spin Resonance Spectroscopy; Ethylenes/biosynthesis; Gene Expression/drug effects; Humans; Hydrocortisone/pharmacology; Hydrogen Peroxide/metabolism; Interleukin-8/antagonists & inhibitors/biosynthesis; Membrane Transport Proteins/biosynthesis; Methylprednisolone/pharmacology; Monocytes/cytology/drug effects/metabolism/microbiology; NADPH Dehydrogenase/biosynthesis; NADPH Oxidase; NF-kappa B/metabolism; Phosphoproteins/biosynthesis; Reactive Oxygen Species/metabolism; Respiratory Burst/drug effects/physiology; Tumor Necrosis Factor-alpha/antagonists & inhibitors/metabolism

Abstract :

[en] We previously observed that the respiratory burst of human monocytes (THP-1 cell line) triggered by phorbol myristate acetate was strongly enhanced by a priming of the cells by Chlamydia pneumoniae [Biochem. Biophys. Res. Commun. 287 (2001) 781]. We describe here the modifications of the responses of Chlamydia-primed THP-1 cells to hydrocortisone (HCT) and methylprednisolone (MPL). HCT and MPL inhibited the production of the cytokines TNFalpha and IL-8. But HCT, which inhibited the respiratory burst in LPS-primed monocytes, paradoxically stimulated the phenomenon in Chlamydia-primed cells; MPL exerted no significant effect. Both glucocorticoids did not significantly modify the triggering effect of Chlamydia on NF-kappaB binding activity. On the expression of p22(phox), a protein subunit of the NADPH oxidase, HCT had an increasing and MPL a decreasing effect. Glucocorticoids thus had unexpected effects on the inflammatory response of Chlamydia-primed monocytes.

Disciplines :

Immunology & infectious disease

Author, co-author :

Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Deby, Ginette ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Mathy, Marianne ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)

Habraken, Yvette ; Université de Liège - ULiège > GIGA-R : Virologie - Immunologie

Nys, Monique ; Centre Hospitalier Universitaire de Liège - CHU > Soins intensifs - Anesthésie et réanimation

Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie

Lamy, Maurice ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation

Deby, Carol

Language :

English

Title :

Effects of glucocorticoids on the respiratory burst of Chlamydia-primed THP-1 cells.

Publication date :

2004

Journal title :

Biochemical and Biophysical Research Communications

ISSN :

0006-291X

eISSN :

1090-2104

Publisher :

Elsevier, Atlanta, United States - California

Volume :

318

Issue :

Pages :

941-8

Peer reviewed :

Peer reviewed

Available on ORBi :

since 06 April 2009

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