ORBi: Mouithys-Mickalad Ange - Effects of glucocorticoids on the respiratory burst of Chlamydia-primed THP-1 cells.

Reference : Effects of glucocorticoids on the respiratory burst of Chlamydia-primed THP-1 cells.


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/10248

Title :	Effects of glucocorticoids on the respiratory burst of Chlamydia-primed THP-1 cells.
Language :	English
Author, co-author :	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Deby, Ginette [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Mathy, Marianne [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
	Habraken, Yvette [Université de Liège - ULg > > GIGA-R : Virologie - Immunologie >]
	Nys, Monique [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs - Anesthésie et réanimation >]
	Henrotin, Yves [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie >]
	Lamy, Maurice [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
	Deby, Carol [> > > >]
Publication date :	2004
Journal title :	Biochemical and Biophysical Research Communications
Volume :	318
Issue/season :	4
Pages :	941-8
Audience :	International
ISSN :	0006-291X
Country :	United States
Keywords :	[en] Anti-Inflammatory Agents/pharmacology ; Cell Differentiation ; Cell Line ; Chlamydophila pneumoniae/metabolism/pathogenicity/physiology ; Electron Spin Resonance Spectroscopy ; Ethylenes/biosynthesis ; Gene Expression/drug effects ; Humans ; Hydrocortisone/pharmacology ; Hydrogen Peroxide/metabolism ; Interleukin-8/antagonists & inhibitors/biosynthesis ; Membrane Transport Proteins/biosynthesis ; Methylprednisolone/pharmacology ; Monocytes/cytology/drug effects/metabolism/microbiology ; NADPH Dehydrogenase/biosynthesis ; NADPH Oxidase ; NF-kappa B/metabolism ; Phosphoproteins/biosynthesis ; Reactive Oxygen Species/metabolism ; Respiratory Burst/drug effects/physiology ; Tumor Necrosis Factor-alpha/antagonists & inhibitors/metabolism
Abstract :	[en] We previously observed that the respiratory burst of human monocytes (THP-1 cell line) triggered by phorbol myristate acetate was strongly enhanced by a priming of the cells by Chlamydia pneumoniae [Biochem. Biophys. Res. Commun. 287 (2001) 781]. We describe here the modifications of the responses of Chlamydia-primed THP-1 cells to hydrocortisone (HCT) and methylprednisolone (MPL). HCT and MPL inhibited the production of the cytokines TNFalpha and IL-8. But HCT, which inhibited the respiratory burst in LPS-primed monocytes, paradoxically stimulated the phenomenon in Chlamydia-primed cells; MPL exerted no significant effect. Both glucocorticoids did not significantly modify the triggering effect of Chlamydia on NF-kappaB binding activity. On the expression of p22(phox), a protein subunit of the NADPH oxidase, HCT had an increasing and MPL a decreasing effect. Glucocorticoids thus had unexpected effects on the inflammatory response of Chlamydia-primed monocytes.
Permalink :	http://hdl.handle.net/2268/10248
	also: http://hdl.handle.net/2268/862
DOI :	10.1016/j.bbrc.2004.04.120

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