Article (Scientific journals)
High prevalence of high-risk human papillomavirus in palatine tonsils from healthy children and adults.
Duray, Anaelle; Descamps, Geraldine; Bettonville, Marie et al.
2011In Otolaryngology - Head and Neck Surgery, 145 (2), p. 230-5
Peer Reviewed verified by ORBi
 

Files


Full Text
Otolaryngology -- Head and Neck Surgery-2011-Duray-230-5.pdf
Publisher postprint (1.05 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] OBJECTIVE: The aim of this study was to determine the prevalence of human papillomavirus (HPV) in 80 tumor-free tonsils from healthy children and adults using a sensitive E6/E7 type-specific polymerase chain reaction (PCR). STUDY DESIGN: Cross-sectional study. SETTING: Ear, nose, and throat department, university hospital. SUBJECTS AND METHODS: Paraffin-embedded tissues from tumor-free tonsils (TFTs) were evaluated for HPV DNA using GP5+/6+ consensus PCR and subsequent genotyping using E6/E7 type-specific PCR for HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, and 68. The immunohistochemical expression of p16 and p53 was also assessed. RESULTS: In 80 TFTs, the authors identified 10 (12.5%) that tested positive for the following high-risk HPV types: HPV 16 (8 cases), 18 (1 case), and 31 (1 case). Twelve patients (15%) tested positive for HPV infection using the GP5+/GP6+ consensus primers but were negative using quantitative PCR. These patients were considered infected with low-risk HPV types. Fifty-eight TFTs (72.5%) tested negative for both GP5+/GP6+ and type-specific HPV PCR analysis (HPV negative). Among patients infected with HPV, the authors observed a slight increase in frequency with age. CONCLUSION: In TFTs, oncogenic and nononcogenic HPVs were present at a relatively high frequency in children and adults. The presence of high-risk HPV DNA in young children supports the horizontal transmission hypothesis and argues in favor of HPV vaccination at birth.
Disciplines :
Oncology
Author, co-author :
Duray, Anaelle
Descamps, Geraldine
Bettonville, Marie
Sirtaine, Nicolas
Ernoux-Neufcoeur, Perle
Guénin, Samuel ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Mouallif, Mustapha ;  Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (Bologne)
Depuydt, Christophe E
Delvenne, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Saussez, Sven
Language :
English
Title :
High prevalence of high-risk human papillomavirus in palatine tonsils from healthy children and adults.
Publication date :
2011
Journal title :
Otolaryngology - Head and Neck Surgery
ISSN :
0194-5998
eISSN :
1097-6817
Publisher :
Mosby Year Book, St Louis, United States - Missouri
Volume :
145
Issue :
2
Pages :
230-5
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 November 2011

Statistics


Number of views
78 (8 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
18
Scopus citations®
without self-citations
17
OpenCitations
 
18

Bibliography


Similar publications



Contact ORBi