Reference : Adipose tissue expression of gelatinases in mouse models of obesity.
Scientific journals : Article
Human health sciences : Endocrinology, metabolism & nutrition
http://hdl.handle.net/2268/102204
Adipose tissue expression of gelatinases in mouse models of obesity.
English
Lijnen, roger [ > > ]
Maquoi, Erik mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Holvoet, P. [ > > ]
Mertens, A. [ > > ]
Lupu, F. [ > > ]
Morange, P. [ > > ]
Alessi, M. C. [ > > ]
Juhan-Vague, I. [ > > ]
Jun-2001
Thrombosis and Haemostasis
Schattauer Gmbh-Verlag Medizin Naturwissenschaften
85
6
1111-1116
Yes (verified by ORBi)
International
0340-6245
Stuttgart
Germany
[en] gelatinase ; obesity ; adipose tissue
[en] Following the observation by Brown et al. (Am J Physiol 1997; 272: C937-49) that primary rat adipocytes in culture secrete gelatinase A (MMP-2), we have evaluated gelatinase expression in adipose tissue with the use of mouse models of obesity. Wild-type mice were kept on a standard fat diet (SFD) or on a high fat diet (42% fat, HFD) and- genetically obese db/db mice were kept on SFD; gonadal and subcutaneous fat pads were removed and analysed ex vivo. These studies revealed that: 1) the HFD induced adipocyte hypertrophy; 2) after 32 weeks, significantly higher levels of 70 kDa (p <0.05) and 65 kDa proMMP-2 (p <0.01) were observed in extracts of gonadal fat pads of mice on HFD; 3) the contribution of active MMP-2 to the total level was comparable in SFD and HFD groups (20 to 30%); and 4) gelatinase B (MMP-9) was not consistently detected. These findings were confirmed by gelatin zymography and by mRNA determination using competitive RT-PCR. The presence of MMP-2 in the adipose tissue was confirmed immunologically and its localization in adipocytes revealed by immunogold electron microscopy. The potential functional role of MMP-2 in adipose tissue remains to be determined.
Researchers ; Professionals
http://hdl.handle.net/2268/102204

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