Article (Scientific journals)
Adipose tissue expression of gelatinases in mouse models of obesity.
Lijnen, roger; Maquoi, Erik; Holvoet, P. et al.
2001In Thrombosis and Haemostasis, 85 (6), p. 1111-1116
Peer Reviewed verified by ORBi
 

Files


Full Text
Adipose Tissue Expression of Gelatinases in Mouse Models of Obesity.pdf
Publisher postprint (266.9 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
gelatinase; obesity; adipose tissue
Abstract :
[en] Following the observation by Brown et al. (Am J Physiol 1997; 272: C937-49) that primary rat adipocytes in culture secrete gelatinase A (MMP-2), we have evaluated gelatinase expression in adipose tissue with the use of mouse models of obesity. Wild-type mice were kept on a standard fat diet (SFD) or on a high fat diet (42% fat, HFD) and- genetically obese db/db mice were kept on SFD; gonadal and subcutaneous fat pads were removed and analysed ex vivo. These studies revealed that: 1) the HFD induced adipocyte hypertrophy; 2) after 32 weeks, significantly higher levels of 70 kDa (p <0.05) and 65 kDa proMMP-2 (p <0.01) were observed in extracts of gonadal fat pads of mice on HFD; 3) the contribution of active MMP-2 to the total level was comparable in SFD and HFD groups (20 to 30%); and 4) gelatinase B (MMP-9) was not consistently detected. These findings were confirmed by gelatin zymography and by mRNA determination using competitive RT-PCR. The presence of MMP-2 in the adipose tissue was confirmed immunologically and its localization in adipocytes revealed by immunogold electron microscopy. The potential functional role of MMP-2 in adipose tissue remains to be determined.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Lijnen, roger
Maquoi, Erik  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Holvoet, P.
Mertens, A.
Lupu, F.
Morange, P.
Alessi, M. C.
Juhan-Vague, I.
Language :
English
Title :
Adipose tissue expression of gelatinases in mouse models of obesity.
Publication date :
June 2001
Journal title :
Thrombosis and Haemostasis
ISSN :
0340-6245
eISSN :
2567-689X
Publisher :
Schattauer Gmbh-Verlag Medizin Naturwissenschaften, Stuttgart, Germany
Volume :
85
Issue :
6
Pages :
1111-1116
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 09 November 2011

Statistics


Number of views
197 (2 by ULiège)
Number of downloads
363 (0 by ULiège)

Scopus citations®
 
55
Scopus citations®
without self-citations
31
OpenCitations
 
48

Bibliography


Similar publications



Contact ORBi