Reference : Comparative sequence analysis of the imprinted Dlk1-Gtl2 locus in three mammalian specie...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/102197
Comparative sequence analysis of the imprinted Dlk1-Gtl2 locus in three mammalian species reveals highly conserved genomic elements and refines comparison with the Igf2-H19 region.
English
Paulsen, M. [> > > >]
Takada, S. [> > > >]
Youngson, N. A. [> > > >]
Benchaib, M. [> > > >]
SEGERS, Karin mailto [Université de Liège - ULg > > CSL (Centre Spatial de Liège)]
Ferguson-Smith, A. C. [ > > ]
Georges, Michel mailto [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale >]
Charlier, Carole mailto [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale >]
2001
Genome Research
Cold Spring Harbor Laboratory Press
11
12
2085-94
Yes (verified by ORBi)
International
1088-9051
1549-5469
Cold Spring Harbor
NY
[en] Animals ; Binding Sites/genetics ; Conserved Sequence/genetics ; CpG Islands/genetics ; DNA-Binding Proteins/genetics/metabolism ; Genetic Markers/genetics ; Genomic Imprinting/genetics ; Humans ; Interspersed Repetitive Sequences/genetics ; Membrane Proteins/genetics ; Mice ; Molecular Sequence Data ; Proteins/genetics/metabolism ; Repressor Proteins ; Sheep ; Transcription Factors/genetics/metabolism
[en] The Dlk1-Gtl2 domain on mouse chromosome 12 contains reciprocally imprinted genes with the potential to contribute to our understanding of common features involved in imprinting control. We have sequenced this conserved region in the mouse and sheep and included the human sequence in a three species comparison. This analysis resulted in a precise conservation map and identification of highly conserved sequence elements, some of which we have shown previously to be differentially methylated in the mouse. Additionally, this analysis facilitated identification of a CpG-rich tandem repeat array located approximately 13-15 kb upstream of Gtl2. Furthermore, we have identified a third imprinted transcript that overlaps with the last Dlk1 exon in the mouse. This transcript lacks a conserved open reading frame and is probably generated by cleavage of extended Dlk1 transcripts. Because Dlk1 and Gtl2 share many of the imprinting properties of the well-characterized Igf2-H19 domain, it has been proposed that the two regions may be regulated in the same way. Comparative genomic examination of the two domains indicates that although there are similarities, other features are very different, including the location of conserved CTCF-binding sites, and the level of conservation at regulatory regions.
http://hdl.handle.net/2268/102197
10.1101/gr.206901

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