Construction and characterization of an ovine BAC contig spanning the callipyge locus.

SEGERS, Karin; Vaiman, D.; Berghmans, S.; Shay, T.; Meyers, S.; Beever, J.; Cockett, N.; Charlier, Carole; Georges, Michel

doi:10.1046/j.1365-2052.2000.00676.x

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Construction and characterization of an ovine BAC contig spanning the callipyge locus.

SEGERS, Karin; Vaiman, D.; Berghmans, S. et al.

2000 • In Animal Genetics, 31 (6), p. 352-9

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https://hdl.handle.net/2268/102189

DOI
10.1046/j.1365-2052.2000.00676.x

PubMed
11167521

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Keywords :

Animals; Base Sequence; Chromosomes, Artificial, Bacterial; DNA Primers; Electrophoresis, Gel, Pulsed-Field; Polymerase Chain Reaction; Sequence Tagged Sites; Sheep/genetics

Abstract :

[en] We describe the construction of an ovine BAC contig spanning a 4.6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7.5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of Not1 sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous +/+ and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation.

Disciplines :

Genetics & genetic processes

Author, co-author :

SEGERS, Karin ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Vaiman, D.

Berghmans, S.

Shay, T.

Meyers, S.

Beever, J.

Cockett, N.

Charlier, Carole

Georges, Michel ; Université de Liège - ULiège > Unit of Animal Genomics

Language :

English

Title :

Construction and characterization of an ovine BAC contig spanning the callipyge locus.

Publication date :

2000

Journal title :

Animal Genetics

ISSN :

0268-9146

eISSN :

1365-2052

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Issue :

Pages :

352-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 09 November 2011

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Bibliography

Baldacci P.A., Richoux V., Renard J.-P., Guénet J.-L., Babinet C. (1992) The locus Om, responsible for the DDK syndrome, maps close to Sigje on mouse chromosome 11. Mammalian Genome 2:100-105.
Beechey C.V., Cattanach B.M. (1994) Viable tertiary trisomy and monosomy within the distal chromosome 12 imprinting region. Mouse Genome 92:505-506.
Carpenter C.E., Rice O.D., Cockett N.E., Snowder G.D. (1996) Histology and composition of muscles from normal and callipyge lambs. Journal of Animal Science 74:388-393.
Cattanach B.M., Raspberry C. (1993) Evidence for imprinting involving the distal region of chromosome 12. Mouse Genome 91:858.
Cockett N.E., Jackson S.P., Shay T.D., Nielsen D., Green R.D., Georges M. (1994) Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proceedings of the National Academy of Sciences USA 91:3019-3023.
Cockett N., Jackson S., Shaw T., Farnir F., Snowder G., Nielsen D., Georges M. (1996) Polar overdominance at the ovine callipyge locus. Science 273:236-238.
Craig J.M., Bickmore W.A. (1994) The distribution of CpG islands in mammalian chromosomes. Nature Genetics 7:376-382.
Freking B.A., Keele J.W., Beattie C.W., Kappes S.M., Smith T.P., Sonstegard T.S., Nielsen M.K., Leymaster K.A. (1998) Evaluation of the ovine callipyge locus: I. Relative chromosomal position and gene action. Journal of Animal Science 76:2062-2071.
Freking B.A., Keele J.W., Nielsen M.K., Leymaster K.A. (1998) Evaluation of the ovine callipyge locus: II. Genotypic effects on growth, slaughter, and carcass traits. Journal of Animal Science 76:2549-2559.
Freking B.A., Keele J.W., Shackelford S.D., Wheeler T.L., Koohmaraie M., Nielsen M.K., Leymaster K.A. (1998) Evaluation of the ovine callipyge locus: III. Genotypic effects on meat quality traits. Journal of Animal Science 77:2336-2344.
Georgiades P., Chierakul C., Ferguson-Smith A.C. (1998) Parental origin effects in human trisomy for chromosome 14q: Implications for genomic imprinting. Journal of Medical Genetics 35:821-824.
Iannuzzi L., Di Meo G.P., Perucatti A., Incarnato D. (1999) Comparison of the human with the sheep genomes by use of human chromosome-specific painting probes. Mammalian Genome 10:719-723.
Jackson S.P., Miller M.F., Green R.D., Brdecko K.S. (1993) Carcass characteristics of Rambouillet ram lambs with genetic muscular hypertrophy., Proceedings of the Western Section of the American Society of Animal Science; 44:167-169.
Jackson S.P., Miller M.F., Green R.D. (1993) The effect of a muscle hypertrophy gene on muscle weights of ram lambs., Proceedings of the Western Section of the American Society of Animal Science; 44:169.
Jackson S.P., Green R.D. (1993) Muscle trait inheritance, growth performance and feed efficiency of sheep exhibiting a muscle hypertropy phenotype., Proceedings of the Western Section of the American Society of Animal Science; 44:364-366.
Lyons A.L., Laughlin T.F., Copeland N.G., Jenkins N.A., Womack J.E., O'Brien S.J. (1996) Comparative anchor tagged sequences for integrative mapping of mammalian genomes. Nature Genetics 15:47-56.
Moore S.S., Sargeant L., King T.J., Mattick J.S., Georges M., Hetzel D.J.S. (1991) The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics 10:654-660.
Paldi A., Gyapay G., Jami J. (1995) Imprinted chromosomal regions of the human genome display sex-specific meiotic recombination frequencies. Current Biology 5:1030-1035.
Sapienza C., Paquette J., Pannunzio P., Albrechtson S., Morgan K. (1992) The polar-lethal ovum mutant gene maps to the distal portion of mouse chromosome 11. Genetics 132:241-246.
Schuster-Gossler K., Simon D., Guénet J.-L., Zachgo J., Gossler A. (1996) Gtl2lacz, an insertional mutation on mouse chromosome 12 with parental origin dependent phenotype. Mammalian Genome 7:20-24.
Schuster-Gossler K., Bilinski P., Sado T., Ferguson-Smith A., Gossler A. (1998) The mouse Gtl2 gene is differentially expressed during embryonic development, encodes multiple alternatively spliced transcripts, and may act as an RNA. Developmental Dynamics 212:214-228.
Shay T.L., Berghmans S., Segers K., Meyers S., Beever J.E., Womack J.E., Georges M., Charlier C., Cockett M.E. (2000) Fine mapping and construction of a bovine contig spaning the ovine callipyge locus. Mammalian Genome, in press; .
Temple I.K., Cockwell A., Hassold T., Pettay D., Jacobs P. (1991) Maternal uniparental disomy for chromosome 14. Journal of Medical Genetics 28:511-514.
Vaiman D., Billault A., Tabet-Aoul K., Schibler L., Vilette D., Outsry-Vaiman A., Soravito C., Cribiu E.P. (1999) Construction and characterisation of a sheep BAC library of three genome equivalents. Mammalian Genome 10:585-587.
Wakasugi N.A. (1974) Genetically determined incompatibility system between spermatozoa and eggs leading to embryonic death in mice. Journal of Reproduction and Fertility 41:85-96.
Wang J.-C.C., Passage M.B., Ye P.H., Shapiro L.J., Mohandas T.K. (1991) Uniparental heterodisomy for chromosome 14 in a phenotypically abnormal familial balanced 13/14 robertsonian translocation carrier. American Journal of Human Genetics 48:1069-1074.