Reference : Construction and characterization of an ovine BAC contig spanning the callipyge locus.
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/102189
Construction and characterization of an ovine BAC contig spanning the callipyge locus.
English
SEGERS, Karin mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique]
Vaiman, D. [> > > >]
Berghmans, S. [> > > >]
Shay, T. [> > > >]
Meyers, S. [> > > >]
Beever, J. [> > > >]
Cockett, N. [> > > >]
Charlier, Carole mailto [ > > ]
Georges, Michel mailto [ > > ]
2000
Animal Genetics
Blackwell Publishing
31
6
352-9
Yes (verified by ORBi)
International
0268-9146
1365-2052
Oxford
United Kingdom
[en] Animals ; Base Sequence ; Chromosomes, Artificial, Bacterial ; DNA Primers ; Electrophoresis, Gel, Pulsed-Field ; Polymerase Chain Reaction ; Sequence Tagged Sites ; Sheep/genetics
[en] We describe the construction of an ovine BAC contig spanning a 4.6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7.5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of Not1 sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous +/+ and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation.
http://hdl.handle.net/2268/102189

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