ORBi: Servotte S. - Establishment of stable human fibroblast cell lines constitutively expressing active Rho-GTPases.

Reference : Establishment of stable human fibroblast cell lines constitutively expressing active Rho...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/10212

Title :	Establishment of stable human fibroblast cell lines constitutively expressing active Rho-GTPases.
Language :	English
Author, co-author :	Servotte, S. [>Université de Liège > > >Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
	Zhang, Z. [>University of Cologne > > >Center for Biochemistry > > >]
	Lambert, Charles [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
	Ho, Thi Thanh Giang [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs > >]
	Chometon, G. [University of Cologne > > >Center for Biochemistry > > >]
	Eckes, B. [>University of Cologne > > >Department of Dermatology > > >]
	Krieg, T. [>University of Cologne > > >Department of Dermatology > Center for Biochemistry > >]
	Lapiere, C. M. [> > > >]
	Nusgens, Betty [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
	Aumailley, M. [>University of Cologne > > >Center for Biochemistry, Center forMolecular Medicine > > >]
Publication date :	2006
Journal title :	Protoplasma
Volume :	229
Issue/season :	2-4
Pages :	215-20
Audience :	International
ISSN :	0033-183X
Country :	Austria
Keywords :	[en] Cell Line ; Cell Proliferation ; Cell Shape ; Enzyme Activation ; Fibroblasts/cytology/enzymology ; Humans ; Microfilaments/metabolism ; Mutation ; Pseudopodia/metabolism ; Time Factors ; Transfection ; Vinculin/metabolism ; cdc42 GTP-Binding Protein/metabolism ; rac1 GTP-Binding Protein/metabolism ; rho GTP-Binding Proteins/genetics/metabolism ; rhoA GTP-Binding Protein/metabolism
Abstract :	[en] Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell's cytoskeleton and adhesion structures. A significant function of these cellular structures is to translate and counterbalance forces applied to, or generated by, cells in order to maintain homeostasis and control cell movement. We therefore hypothesised that Rho-GTPases are directly involved in cellular gravity perception and may participate in the alterations induced in microgravity. To define an adequate cellular model allowing to investigate this issue, we have established stable cell lines constitutively expressing active forms of either RhoA, Cdc42, or Rac1. The three cell lines differ by morphology and by their ability to form filopodia, lamellipodia, and bundles of actin stress fibers. Overexpression of the active form of either RhoA, Cdc42, or Rac1 is compatible with cell viability and does not affect cell population doubling time. Thus, our series of mutant cells appear well suited to gain further knowledge on the molecular mechanisms of cellular gravity perception.
Target :	Researchers ; Students
Permalink :	http://hdl.handle.net/2268/10212
DOI :	10.1007/s00709-006-0204-0
Commentary :	The original publication is available at www.springerlink.com

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