[en] Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell's cytoskeleton and adhesion structures. A significant function of these cellular structures is to translate and counterbalance forces applied to, or generated by, cells in order to maintain homeostasis and control cell movement. We therefore hypothesised that Rho-GTPases are directly involved in cellular gravity perception and may participate in the alterations induced in microgravity. To define an adequate cellular model allowing to investigate this issue, we have established stable cell lines constitutively expressing active forms of either RhoA, Cdc42, or Rac1. The three cell lines differ by morphology and by their ability to form filopodia, lamellipodia, and bundles of actin stress fibers. Overexpression of the active form of either RhoA, Cdc42, or Rac1 is compatible with cell viability and does not affect cell population doubling time. Thus, our series of mutant cells appear well suited to gain further knowledge on the molecular mechanisms of cellular gravity perception.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Servotte, S.; Université de Liège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Zhang, Z.; University of Cologne > Center for Biochemistry
Lambert, Charles ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Ho, Thi Thanh Giang ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Chometon, G.; University of Cologne > Center for Biochemistry
Eckes, B.; University of Cologne > Department of Dermatology
Krieg, T.; University of Cologne > Department of Dermatology > Center for Biochemistry
Lapiere, C. M.
Nusgens, Betty ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs
Aumailley, M.; University of Cologne > Center for Biochemistry, Center forMolecular Medicine
Language :
English
Title :
Establishment of stable human fibroblast cell lines constitutively expressing active Rho-GTPases.
Publication date :
2006
Journal title :
Protoplasma
ISSN :
0033-183X
eISSN :
1615-6102
Publisher :
Springer, Germany
Volume :
229
Issue :
2-4
Pages :
215-20
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
The original publication is available at www.springerlink.com
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