Article (Scientific journals)
What role is there for antithymocyte globulin in allogeneic nonmyeloablative canine hematopoietic cell transplantation?
Diaconescu, Razvan; Little, Marie-Terese; Leisenring, Wendy et al.
2005In Biology of Blood and Marrow Transplantation, 11 (5), p. 335-44
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Keywords :
Animals; Antilymphocyte Serum/therapeutic use; Bone Marrow Transplantation; Dogs; Graft Rejection; Graft Survival; Hematopoietic Stem Cell Transplantation/methods; Immunosuppressive Agents/therapeutic use; Models, Animal; Transplantation Chimera; Transplantation Conditioning/methods; Transplantation, Homologous; Whole-Body Irradiation
Abstract :
[en] We investigated whether pretransplantation immunosuppression with canine-specific rabbit antithymocyte globulin (ATG), combined with a suboptimal dose of 1 Gy of total body irradiation (TBI), would permit engraftment of canine dog leukocyte antigen-identical marrow. Cumulative ATG doses of 2 to 5 mg/kg produced a T-cell depletion of 1 log in the peripheral blood and 50% in the lymph nodes. Serum levels of ATG peaked on days 4 to 6 after initiation of therapy and became undetectable by day 13 as a result of canine antibody responses to ATG. ATG prolonged allogeneic skin graft survival to 14 days (n = 5), compared with 8 days in control dogs (P = .0003). Five dogs were given marrow transplants after ATG (3.5-5 mg/kg) and 1 Gy of TBI. Posttransplantation immunosuppression consisted of mycophenolate mofetil and cyclosporine. All dogs showed initial engraftment, with maximum donor chimerism levels of 25%. However, only 1 dog achieved sustained engraftment, and 4 rejected their grafts. The duration of engraftment ranged from 8 to > or = 36 weeks (median, 11 weeks), and this is comparable to that in 6 historical controls not given ATG (range, 3-12 weeks; median, 10 weeks; P = .20). The total nucleated cell doses in the marrow grafts had the highest correlation coefficient with the duration of engraftment: 0.82 (P = .09). We concluded that administering ATG before an otherwise suboptimal conditioning dose of 1 Gy of TBI failed to secure uniform stable hematopoietic engraftment.
Disciplines :
Hematology
Author, co-author :
Diaconescu, Razvan
Little, Marie-Terese
Leisenring, Wendy
Yunusov, Murad
Hogan, William J
Sorror, Mohamed L
Baron, Frédéric  ;  Université de Liège - ULiège > Département des sciences cliniques > GIGA-R : Hématologie - Département des sciences cliniques
Storb, Rainer
Language :
English
Title :
What role is there for antithymocyte globulin in allogeneic nonmyeloablative canine hematopoietic cell transplantation?
Publication date :
2005
Journal title :
Biology of Blood and Marrow Transplantation
ISSN :
1083-8791
eISSN :
1523-6536
Publisher :
Carden Jennings Publishing, Charlottesville, United States - Virginia
Volume :
11
Issue :
5
Pages :
335-44
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 09 November 2011

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