Reference : Demonstrating polymorphic miRNA-mediated gene regulation in vivo: application to the ...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/101986
Demonstrating polymorphic miRNA-mediated gene regulation in vivo: application to the g+6223G->A mutation of Texel sheep.
English
Takeda, Haruko mailto [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale]
Charlier, Carole mailto [Université de Liège - ULg > Département de productions animales > GIGA-R : Génomique animale]
Farnir, Frédéric mailto [Université de Liège - ULg > Département de productions animales > Biostatistiques et bioinformatique appliquées aux sc. vétér.]
Georges, Michel mailto [ > > ]
2010
RNA (New York, N.Y.)
RNA Society
16
9
1854-63
Yes (verified by ORBi)
International
1355-8382
1469-9001
[en] 3' Untranslated Regions ; Animals ; Gene Expression Regulation ; Genetic Techniques ; Hela Cells ; Heterozygote ; Humans ; MicroRNAs/metabolism ; Muscle, Skeletal/metabolism ; Mutation ; Myostatin/genetics ; Polymorphism, Single Nucleotide ; RNA-Induced Silencing Complex/metabolism ; Sheep, Domestic/genetics
[en] We herein describe the development of a biochemical method to evaluate the effect of single nucleotide polymorphisms (SNPs) in target genes on their regulation by microRNAs in vivo. The method is based on the detection of allelic imbalance in RNAs coimmunoprecipitated with AGO proteins from tissues of heterozygous individuals. We characterize the performances of our approach using a model system in a cell culture, and then apply it successfully to prove that the 3'UTR g+6223G-->A mutation operates by promoting RISC-dependent down-regulation of myostatin (MSTN) in skeletal muscle of Texel sheep.
Researchers ; Professionals
http://hdl.handle.net/2268/101986
10.1261/rna.2131110

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