Reference : Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative tra...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/101941
Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition
English
Grisart, B. [> > > >]
Farnir, Frédéric mailto [Université de Liège - ULg > Département de productions animales > Biostatistique, économie, sélection animale >]
Karim, Latifa mailto [Université de Liège - ULg > Département de productions animales > Génomique animale >]
Cambisano, Nadine mailto [Université de Liège - ULg > Département de productions animales > Génomique animale >]
Kim, J. J. [> > > >]
Kvasz, A. [> >]
Mni, Myriam mailto [Université de Liège - ULg > Département de productions animales > Génomique animale >]
Simon, P. [> >]
Frère, J. M. [> >]
Coppieters, Wouter mailto [Université de Liège - ULg > Département de productions animales > Département de productions animales >]
Georges, Michel mailto [Université de Liège - ULg > Département de productions animales > Génomique animale >]
2004
Proceedings of the National Academy of Sciences of the United States of America
Natl Acad Sciences
101
8
2398-2403
Yes (verified by ORBi)
International
0027-8424
Washington
[en] We recently used a positional cloning approach to identify a nonconservative lysine to alanine substitution (K232A) in the bovine DGAT1 gene that was proposed to be the causative quantitative trait nucleotide underlying a quantitative trait locus (QTL) affecting milk fat composition, previously mapped to the centromeric end of bovine chromosome 14. We herein generate genetic and functional data that confirm the causality of the DGAT1 K232A mutation. We have constructed a high-density single-nucleotide polymorphism map of the 3.8-centimorgan BULGE30-BULGE9 interval containing the QTL and show that the association with milk fat percentage maximizes at the DGAT1 gene. We provide evidence that the K allele has undergone a selective sweep. By using a baculovirus expression system, we have expressed both DGAT1 alleles in Sf9 cells and show that the K allele, causing an increase in milk fat percentage in the live animal, is characterized by a higher V-max in producing triglycerides than the A allele.
Researchers ; Professionals
http://hdl.handle.net/2268/101941

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