ORBi: Gerkens Pascal - Apoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells

Reference : Apoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Anatomy (cytology, histology, embryology...) & physiology
	To cite this reference:	http://hdl.handle.net/2268/10182

Title :	Apoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells
Language :	English
Author, co-author :	Gerkens, Pascal [ > > ]
	Dobson, Rowan [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
	tabatadze, Nino [ > > ]
	Mshviladzade, Vakhtang [ > > ]
	Elias, Riad [ > > ]
	Peulen, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Jolois, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Publication date :	11-Jun-2007
Journal title :	Anticancer Research
Publisher :	JG Delinassios Anticancer Research
Volume :	27
Pages :	2529-2534
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0250-7005
City :	Athens
Country :	Greece
Keywords :	[en] HL- 60 ; apoptosis ; hederacolchiside
	[fr] giganteoside ; cytolysis
Abstract :	[en] The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells. Materials and Methods: the end-point metabolic (WST1) and lactate dehydrogenase (LDH) assays were used. Cell cycle analysis and apoptosis were measured by flow cytometry, DNA laddering and caspase 3 analyses. Results: the HL-60 cell line was more sensitive to Hcol-A1 and Gig-D (IC50 3-5 ÌM) than to Gig-E and Hcol-A (IC50 8-13 ÌM; WST1 assay). This was related to LDH release. The induction of apoptosis could be detected without caspase 3 activation after 24 h of treatment. DNA fragmentation could be detected only with Gig-D. With Hcol- A1 and Gig-D, an accumulation of cells in the S-phase and an increase of cells in sub-G1 peak were observed. By the annexinV-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (AAD) assay, the majority of cells were in late apoptosis with Gig-D, and in necrosis with Hcol-A1. Conclusion: Hcol-A1 is more cytotoxic than Gig-D, followed by Gig-E and finally Hcol-A. This is related to a membrane permeabilization effect, leading to cytolysis
Research units :	Giga-Cancer ; Centre Interfacultaire d'Analyse des Résidus en Traces - CART
Funders :	International Association for the Promotion of Cooperation with Scientists from the New Independent States of the Former Soviet Union - INTAS
Name of the research project :	97-491
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/10182

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