Reference : Light and electron microscopic immunolocalization of bovine pregnancy-associated glyc...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Human health sciences : Endocrinology, metabolism & nutrition
http://hdl.handle.net/2268/10109
Light and electron microscopic immunolocalization of bovine pregnancy-associated glycoprotein in the bovine placentome.
English
Zoli, A. [> >]
Demez, Pierre [Université de Liège - ULg > > O.R.L. >]
Beckers, Jean-François [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Reznik, Michel [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Beckers, Albert mailto [Université de Liège - ULg > Département des sciences cliniques > Endocrinologie >]
1992
Biology of Reproduction
Society for the Study of Reproduction
46
4
623-9
Yes (verified by ORBi)
International
0006-3363
1529-7268
Madison
WI
[en] Animals ; Cattle ; Chorionic Villi/chemistry/metabolism/ultrastructure ; Female ; Glycoproteins/analysis/metabolism ; Immunohistochemistry ; Microscopy, Electron ; Placenta/chemistry/metabolism/ultrastructure ; Pregnancy ; Pregnancy Proteins/analysis/metabolism ; Pregnancy, Animal/metabolism
[en] A bovine pregnancy-associated glycoprotein (bPAG) of 67 kDa has previously been isolated from bovine fetal cotyledons. The objective of this study was to determine the cytological localization of that protein in the placentomes and possibly the cells responsible for its production. Highly specific antisera raised against pure bPAG were used to demonstrate the cellular localization of the protein in bovine placentomes by light and electron microscopic techniques. Strong immunostaining was observed exclusively in the cytoplasm of large binucleate cells present predominantly in fetal cotyledonary tissue (villi). Some smaller weakly immunostained cells were also present in caruncular epithelium. By ultrastructural immunogold procedures, the protein was detected only within amorphous cytoplasmic granules. Granules of identical size, but weakly labeled, were found on the maternal side. All cells containing labeled granules were binucleate. These results suggest that bPAG is probably synthesized by trophoblast binucleate cells and stored in granules prior to delivery into the maternal circulation after cell migration.
Laboratoire d'Endocrinologie et de Physiologie de la Reproduction animale
I.R.S.I.A.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/10109
also: http://hdl.handle.net/2268/5797
10.1095/biolreprod46.4.623

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