[en] We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and placental lactogen (hCS-B). We have cloned these genes and are searching within their sequences for in vitro binding sites of the human glucocorticoid receptor on the hGH-N and hCS-B genes; the in vivo activity of such DNA sequences by assaying hybrid gene expression in transfected cells; in vivo "enhancer" activity of different hPRL gene fragments linked to a marker gene and transfected in cultured cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Belayew, A.
Bellefroid, Eric J.
Berwaer, M.
Dumoulin, M.
Lambert, C.
Lemaitre-Wathy, C.
Mathy-Hartert, M.
Morin, A.
Pasleau, Françoise ; Université de Liège - ULiège > CARE "Le Réseau des bibliothèques" > Bibliothèque des Sciences de la vie
Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Language :
French
Title :
Localisation des séquences régulatrices de la transcription. Application aux gènes de la famille prolactine.
Alternative titles :
[en] Localization of transcription regulatory sequences. Application to the genes of the prolactin family.
