Reference : Cytosine Deaminase Suicide Gene Therapy for Peritoneal Carcinomatosis
Scientific journals : Article
Human health sciences : Hematology
http://hdl.handle.net/2268/10054
Cytosine Deaminase Suicide Gene Therapy for Peritoneal Carcinomatosis
English
Bentires-Alj, M. [> > > >]
Hellin, A. C. [> > > >]
Lechanteur, Chantal mailto [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie clinique >]
Princen, Frédéric [> >]
Lopez Y Cadenas, Miguel mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Fillet, Georges mailto [Université de Liège - ULg > Département des sciences cliniques > Hématologie - Oncologie médicale]
Gielen, Jean-Louis mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chirurgie maxillo-faciale et plastique >]
Merville, Marie-Paule mailto [Université de Liège - ULg > Département de pharmacie > Chimie médicale >]
Bours, Vincent mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Génétique générale et humaine]
Jan-2000
Cancer Gene Therapy
7
1
20-6
Yes (verified by ORBi)
International
0929-1903
[en] Gene therapy is a novel therapeutic approach that might soon improve the prognosis of some cancers. We investigated the feasibility of cytosine deaminase (CD) suicide gene therapy in a model of peritoneal carcinomatosis. DHD/K12 colorectal adenocarcinoma cells transfected in vitro with the CD gene were highly sensitive to 5-fluorocytosine (5-FC), and a bystander effect could also be observed. Treating CD+ cells with 5-FC resulted in apoptosis as detected by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick-end labeling. In vitro, several human cell lines derived from ovarian or colorectal carcinomas, as well as the rat glioblastoma 9 L cell line, responded to CD/5-FC and showed a very strong bystander effect. 5-FC treatment of peritoneal carcinomatosis generated in syngeneic BDIX rats by CD-expressing DHD/K12 cells led to a complete and prolonged response and to prolonged survival. Our study thus demonstrated the efficacy of CD suicide gene therapy for the treatment of peritoneal carcinomatosis.
http://hdl.handle.net/2268/10054

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