Reference : Presence of gd TcR+ Tumor Infiltrating Lymphocytes (TILs) in One Case of Pediatric Os...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Microbiology
Human health sciences : Oncology
http://hdl.handle.net/2268/100009
Presence of gd TcR+ Tumor Infiltrating Lymphocytes (TILs) in One Case of Pediatric Osteogenic Sarcoma
English
Moutschen, Michel mailto [Université de Liège - ULg > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén. >]
Jacobs, Nathalie mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Hoyoux, Claire [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Martin-Simonet, Marie-Thèrèse [> >]
Schaaf-Lafontaine, Nicole mailto [Centre Hospitalier Universitaire de Liège - CHU > > Hématologie biologique et immuno hématologie >]
Boniver, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
1994
International Journal of Pediatric Hematology/Oncology
Taylor and Francis
Yes (verified by ORBi)
International
1070-2903
[en] Innate lymphocyte ; cancer ; TIL
[en] To characterize ab and gd TcR expression in cultured TILs from a metastatic osteogenic sar- coma.Patient and methods: TILs were isolated from a lung metastasis of an osteogenic sarcoma in a 16- year-old female patient. Culture conditions were IL- 2 and anti-CD3 MoAb+IL-2. TcR expression and surface phenotype were studied by flow cytometry. 4h-chromium release assays were used to assess cytotoxicity.
Results:IL-2-expanded tumor infiltrating T-lym- phocytes contained 50% gd TcR+ cells whereas gd TcR+ cells represented 20% of T-cells in cultures expanded with anti-CD3 MoAb+IL-2. The phenotype of gd TcR+ cells was CD3+, CD16-, CD4-, CD8-, and CD29+. AnH-CD3 MoAb + IL-2-expanded TILs mediated a significant lysis of thé autologous tumor and of an allogeneic lymphoma and displayed a higher activity against thé NK-sensitive target K562. In cold target experiments, unlabeled autol- ogous tumor cells blocked thé lysis of K562, indicating that MHC-unrestricted effectors were involved in thé lysis of both targets.
Conclusions: This report demonstrates thé ex- pansion of large numbers of gd TcR+ T-cells after short-term culture of TILs from a metastatic os- teosarcoma. Further studies are needed to déter- mine thé role of gd TcR+ cells in host immune responses directed against this tumor.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals
http://hdl.handle.net/2268/100009

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