http://hdl.handle.net/2268/146 Search this channel search http://orbi.ulg.ac.be/simple-search http://hdl.handle.net/2268/148611 Title: Photobioreactors for efficient production of molecules with high added value, based upon an innovative technology for the production of porous materials <br/> <br/>Author, co-author: Tocquin, Pierre Thu, 16 May 2013 19:13:52 GMT http://hdl.handle.net/2268/148477 Title: Anatomie et identification des bois <br/> <br/>Author, co-author: Jourez, Benoît <br/> <br/>Abstract: Anatomie des bois Structure anatomique des résineux et des feuillus Structure de la membrane cellulaire structure submicroscopique Anatomie du bois des essences feuillues tropicales Caractères anatomiques servant à l'identification des essences Reconnaissance microscopique du bois des essences résineuses et feuillues Duramen et duraminisation Formations anormales ( bois de compression et bois de tension) Chimie du bois Composition générale Cellulose, hémicellulose, lignine, constituants accessoires; WOOD ANATOMY Anatomical structure of softwoods and hardwoods Structure of cell wall and submicroscopical structure Wood anatomy of tropical hardwood species Anatomical features leading to the identification of wooden species Heartwood and heartwood formation Abnormal structure (compression wood and tension wood) WOOD CHEMISTRY General composition Cellulose, hemicellulose, lignine and secondary components. Tue, 14 May 2013 14:38:31 GMT http://hdl.handle.net/2268/148476 Title: Coconut lumber for wood decks (Cocos nucifera L.):decay resistance against Basidiomycetes fungi <br/> <br/>Author, co-author: Jourez, Benoît; Verheyen, Cécile; Van Acker, Joris <br/> <br/>Abstract: Since a couple of years, manufactured products of coconut wood for outdoor uses like wood decks have been proposed on the European market. These are presented as an alternative for traditional tropical timbers. In the past, coconut wood was neglected and burned for sanitary reasons and lack of interest at industrial scale. Plantation coconut trees at end of production of copra constitute a renewable resource with high added value. In order to convince the markets, natural durability for outdoor use, without preservative treatment, against wood destroying fungi characteristic of northern temperate regions is a major property that has to be checked. Natural durability of coconut lumber was tested in the laboratory according to the European standard EN 15083-1 against brown (Coniophora puteana) and white (Coriolus versicolor) rot decay basidiomycetes fungi. Beech wood specimens were used as virulence controls. Mass losses were determined after 16 weeks exposure. The results showed that tested coconut wood is very resistant to the brown rot fungus Coniophora puteana and resistant to the white rot fungus Coriolus versicolor. Mass loss and density of tested samples with C. versicolor are inversely related. In addition, the density shows a large variability in the test sample, more than 500 kg / m³. These results confirm that after a selection on density criteria, the natural durability of Coconut lumber is sufficient for outdoor application in use class 3 (based on European standard EN 335 and in line with EN 460). Tue, 14 May 2013 14:36:36 GMT http://hdl.handle.net/2268/148470 Title: Complex regulation of the FRD3 gene in Arabidopsis relatives <br/> <br/>Author, co-author: Charlier, Jean-Benoit; Polese, Catherine; Nouet, Cécile; Krämer, Ute; Motte, Patrick; Hanikenne, Marc Tue, 14 May 2013 13:20:24 GMT http://hdl.handle.net/2268/148452 Title: Stockage de bois chablis sous bâche <br/> <br/>Author, co-author: Castaings, Frédéric; Jourez, Benoît; Riguelle, Simon <br/> <br/>Abstract: Le samedi 24 janvier 2009, la tempête Klaus traverse le sud-ouest de la France. Tempête la plus dévastatrice depuis 1999, la forêt des Landes et ses pins maritimes paient un lourd tribut à ce phénomène extrême: 60% de la superficie est touchée, environ 40 millions de m3 de bois sont à terre, quatre fois la récolte annuelle. Il faudra attendre plusieurs mois avant que les aires de stockage par aspersion soient opérationnelles. Trop tard, le pin maritime montre déjà des signes de bleuissement de son bois, il est invendable sur les marchés à forte valeur ajoutée, là où l’esthétisme prime. Écarté des meilleurs débouchés, le lambris et le plancher, le manque à gagner est énorme. Un tel scénario catastrophe, notre région s’y prépare depuis 2005, tirant les enseignements des expériences étrangères et fondant son action sur les spécificités de notre forêt, de notre filière. C’est dans ce cadre que le 28 février dernier, à Eupen, des grumes d’épicéa stockées depuis presque cinq ans ont été extraites des bâches hermétiques qui les protégeaient. Comment le bois est-il ressorti de cette épreuve? Que penser de cette méthode alternative de stockage? Quelle place lui réserver ? Benoit Jourez du Laboratoire de Technologie du Bois du DEMNA (Département de l’Étude du milieu naturel et agricole) nous a apporté son éclairage et nous a conviés à l’ouverture de la poche étanche. Entre supputations et réalité des bois extraits après cinq ans, suspense… <br/> <br/>Commentary: L'article propose un compte rendu de la journée de démonstration aux professionnels de la filière bois. Il peut être consulté à l'adresse suivante: http://www.rnd.be/publications/les-infos-de-rnd/ Une vidéo présentant les moments forts de la journée peut être vue sur le site www.rnd.be Tue, 14 May 2013 10:37:25 GMT http://hdl.handle.net/2268/148440 Title: Biocontrol and other alternative control methods against pre-and post-harvest pome fruit diseases. <br/> <br/>Author, co-author: Jijakli, Haissam Tue, 14 May 2013 09:21:29 GMT http://hdl.handle.net/2268/148253 Title: Flora and vegetation of Turtell V, Turtell Islands group (Queensland, Australia). <br/> <br/>Author, co-author: De Sloover, Jacques; Dufrêne, Marc Sun, 12 May 2013 08:42:05 GMT http://hdl.handle.net/2268/147889 Title: Valorisation des produits issus des feuilles de betteraves : extraction de molécules à note verte <br/> <br/>Author, co-author: Marlier, Michel; Thonart, Philippe; du Jardin, Patrick; Ongena, Marc; Fauconnier, Marie-Laure Fri, 03 May 2013 14:43:46 GMT http://hdl.handle.net/2268/147888 Title: Valorisation des produits issus des feuilles de betteraves : extraction de molécules à note verte - Rapport final <br/> <br/>Author, co-author: Wathelet, Jean-Paul; Thonart, Philippe; du Jardin, Patrick; Ongena, Marc; Fauconnier, Marie-Laure Fri, 03 May 2013 14:43:21 GMT http://hdl.handle.net/2268/147887 Title: Valorisation des produits issus des feuilles de betteraves : extraction de molécules à note verte <br/> <br/>Author, co-author: Wathelet, Jean-Paul; Thonart, Philippe; du Jardin, Patrick; Ongena, Marc; Fauconnier, Marie-Laure Fri, 03 May 2013 14:42:56 GMT http://hdl.handle.net/2268/147886 Title: Recherches de lignées aromatiques d'Abies par hybridation somatique <br/> <br/>Author, co-author: Wathelet, Jean-Paul; Fauconnier, Marie-Laure; Michels, Franck; Misson, Jean-Pierre Fri, 03 May 2013 14:42:24 GMT http://hdl.handle.net/2268/147823 Title: IMPACT OF HEAVY METALS ON PHYSIOLOGICAL AND PROTEOMIC RESPONSES OF WILLOWS (SALIX SP.) <br/> <br/>Author, co-author: Evlard, Aricia; Sergeant, Kjell; Ferrandis, Salvador; Renaut, Jenny; Guignard, Cedric; Paul, Roger; Hausman, Jean-François; Campanella, Bruno <br/> <br/>Abstract: In 2010, around 3,800 sites in Wallonia (Belgium) were identified as potentially contaminated by heavy metals, a result of their past industrial and agricultural activities [1]. The technique of using plants to remediate contaminated sites (phytoremediation) has been studied for over twenty years. In particular, the use of trees (alder, willow, poplar) has been considered because of their large biomass production [2] [3] [4] [5]. The aim of this study was to identify the potential of local willow ecotypes to grow in the presence of heavy metals using lysimeters filled with dredging sludge. Several willow ecotypes were compared realizing morphological and physiological measurements (chlorophyll fluorescence, electrolyte leakage, carbohydrate content). The plant responses to metal stress were also investigated using a proteomic approach. Heavy metals contents in leaves and stems were analysed. With this study, the potential of these trees to valorise contaminated, abandoned sites in Belgium was evaluated. [1] Cellule Etat de l'environnement wallon (2010). Tableau de bord de l'environnement wallon. SPW-DGARNE-DEMNA-DEE. [2] Dickinson N. M. (2000). Strategies for sustainable woodland on contaminated soils. Chemosphere 41(1-2): 259-263. [3] Meers E., Lamsal S., Vervaeke P., Hopgood M., Lust N. and Tack F. M. G. (2005). Availability of heavy metals for uptake by Salix viminalis on a moderately contaminated dredged sediment disposal site. Environ Pollut 137(2): 354-364. [4] Meers E., Vandecasteele B., Ruttens A., Vangronsveld J. and Tack F. M. G. (2007). Potential of five willow species (Salix spp.) for phytoextraction of heavy metals. Environ Exp Bot 60(1): 57-68. [5] Rosselli W., Keller C. and Boschi K. (2003). Phytoextraction capacity of trees growing on a metal contaminated soil. Plant Soil 256(2): 265-272. Thu, 02 May 2013 14:33:29 GMT http://hdl.handle.net/2268/147820 Title: Potential of kenaf (Hibiscus cannabinus L.) and corn (Zea mays L.) for phytoremediation of dredging sludge contaminated by trace metals <br/> <br/>Author, co-author: Arbaoui, Sarra; Evlard, Aricia; Mhamdi, Mohamed El Wafi; Campanella, Bruno; Paul, Roger; Bettaieb, Taoufik <br/> <br/>Abstract: The potential of kenaf (Hibiscus cannabinusL.) and corn (Zea mays L.) for accumulation of cadmium and zinc was investigated. Plants have been grown in lysimetres containing dredging sludge, a substratum naturally rich in trace metals. Biomass production was determined. Sludge and water percolating from lysimeters were analyzed by atomic absorption spectrometry.No visible symptoms of toxicity were observed during the three- month culture. Kenaf and corn tolerate trace metals content in sludge. Results showed that Zn and Cd were found in corn and kenaf shoots at different levels,2.49 mg/kg of Cd and 82.5 mg/kg of Zn in kenaf shoots and2.1mg/kgofCdand10.19mg/kgincornshoots. Quantities of extracted trace metals showed that decontamination of Zn and Cd polluted substrates is possible by corn and kenaf crops. Tolerance and bioaccumulation factors indicated that both species could be used in phytoremediation. Thu, 02 May 2013 14:00:36 GMT http://hdl.handle.net/2268/147773 Title: Le potentiel du saule pour la phytostabilisation des sols pollués par les éléments-traces métalliques <br/> <br/>Author, co-author: Evlard, Aricia <br/> <br/>Abstract: Since the ‘80s, when the concept of phytoremediation first appeared, a lot of research has been put into studying the efficiency of woody plants in metal extraction. Willows, as fast growing plants and because of their tolerance to difficult edaphic conditions, have been particularly well investigated. In this investigation, the essays were done on Salix clones, which come from a Walloon collection provided by ECOLIRI and ECOLIRIMED projects. The first objective was to study the potential of these local clones by considering not only their ability to extract their pollutants, but also by adding their biomass production to this parameter. We have called the study of these criteria the phenotypic approach to the tolerance of Salix clones to metals. The second added value of this investigation lies in the second objective which aims to complete these phenotypic criteria with physiological and proteomic criteria. These last criteria are often used to study the metal tolerance of plant species, but rarely for willows. The combination of these different approaches gives a expand view of metal tolerance in Salix clones studied in this investigation. As the phenotypical parameters help to answer the extraction ability challenge, the physiological and proteomic approaches give answers linked to the “health” of the willow trees when they grow in the presence of metals. Our results indicate that the clones that produced more biomass were the ones that showed the highest metal concentrations. The clones with lower biomass production showed the same tolerance as the highest producers and our results revealed that growth reduction indicates metal tolerance. Finally, after comparing our results of the metal concentrations obtained in the twigs, to results obtained during the last two decades of research papers, we have concluded that we should reconsider the use of Salix potential in phytoextraction. The first chapter of this investigation was about Salix clones exposed to metals, but, in natural conditions, their roots are colonized by fungi. Thus, their rhizosphere constitutes a separate ecosystem, which is interesting to investigate. The rhizospheric fungi, the first interface between roots and soil pollutants, play an important role in metal tolerance in woody plants. For this reason, the second chapter of this thesis aimed to test the in vitro growth of rhizospheric fungi collected on woody plant roots in the presence of cadmium. The outcome of these essays is that fungal strains have been identified and classified as tolerant to this metal. This chapter thus constitutes a first step in a future study aiming to analyze these strains in association with woody plant roots in the presence of metals. <br/> <br/>Commentary: Thèse par articles (français, anglais) Thu, 02 May 2013 09:13:32 GMT http://hdl.handle.net/2268/147333 Title: Bioraffinerie végétale : chimie et technologie des structures osidiques (TECHNOSE) <br/> <br/>Author, co-author: Michiels, Franck; Paquot, Michel; Wathelet, Jean-Paul; Fauconnier, Marie-Laure; Blecker, Christophe; Declerck, Stephan; Brasseur, Robert Thu, 25 Apr 2013 11:52:31 GMT http://hdl.handle.net/2268/147140 Title: Towards identification of active root-secreted proteases of Arabidopsis thaliana. <br/> <br/>Author, co-author: Lallemand, Jérôme; Désiron, Carole; Périlleux, Claire; Tocquin, Pierre <br/> <br/>Abstract: Besides traditional production systems, such as bacteria, yeasts and mammal cells, plants can now be used to produce eukaryotic recombinant proteins. Their advantages as hosts for proteins production include correct post-translational modifications, low cost of maintenance and no risk of contamination by human pathogens. Targeting heterologous proteins to the extracellular space is required for the correct folding of complex proteins and makes harvesting and purification easier. However, the quantity and the quality of recombinant proteins have been proved to be reduced by the action of endogenous co-secreted proteases. In this study, we characterized root-secreted proteases in the model plant Arabidopsis thaliana, at the activity and expression levels. Their activity was analyzed by in vitro degradation of a target protein (Bovine Serum Albumine, BSA) in a range of pH and in the presence of several proteases inhibitors. Serine proteases were identified as the major protease class involved in the degradation of BSA under all tested conditions. As a first step towards the identification of the key players, the expression level of selected members of this class was analyzed by quantitative RT-PCR in roots and leaves. Fri, 19 Apr 2013 12:05:02 GMT http://hdl.handle.net/2268/147136 Title: Molecular analysis of root medium impact on Arabidopsis thaliana development <br/> <br/>Author, co-author: Bouché, Frédéric; André, Julie; Tocquin, Pierre; Périlleux, Claire <br/> <br/>Abstract: Hydroponics and soil are the most common media used for plant growth. Hydroponics has the main advantage of providing easy access to the root system and is therefore commonly used for gene expression analyses in molecular studies of the model plant Arabidopsis thaliana. However, the impact of root substrate on plant growth remains poorly documented. Here we show that hydroponics accelerates both shoot growth and developmental phases as compared with culture on soil. In order to identify molecular changes in the roots that could account for these medium effects, a transcriptomic comparison was performed by microarray analysis. This experiment revealed that more than 20% of the genes were differentially expressed in hydroponics vs soil. Among them, the flowering time gene FLOWERING LOCUS C and two clades of microRNA targeted genes. To further assess the role of these genes in roots, artificial microRNAs were designed for root specific expression in transgenic Arabidopsis plants. Fri, 19 Apr 2013 10:04:46 GMT http://hdl.handle.net/2268/146894 Title: A root chicory MADS-box sequence and the Arabidopsis flowering repressor FLC share common features that suggest conserved function in vernalization and devernalization responses <br/> <br/>Author, co-author: Périlleux, Claire; Pieltain, Alexandra; Jacquemin, Guillaume; Bouché, Frédéric; Detry, Nathalie; D'Aloia, Maria; Thiry, Laura; Aljochim, Pierre; Delansnay, Martin; Mathieu, Anne-Sophie; Lutts, Stanley; Tocquin, Pierre <br/> <br/>Abstract: Root chicory (Cichorium intybus var. sativum) is a biennial crop, but is harvested for root inulin at the end of the first growing season before flowering. However, cold temperatures might vernalize seeds or plantlets, leading to incidental early flowering and hence understanding the molecular basis of vernalization is important. A MADS-box sequence was isolated by RT-PCR and named FLC-LIKE1 (CiFL1) because of its phylogenetic positioning within the same clade as the floral repressor Arabidopsis FLOWERING LOCUS C (AtFLC). Moreover, overexpression of CiFL1 in Arabidopsis caused late flowering and prevented up-regulation of the AtFLC target FLOWERING LOCUS T gene by photoperiod, suggesting functional conservation between root chicory and Arabidopsis. Like AtFLC in Arabidopsis, CiFL1 was repressed during vernalization of seeds or plantlets of chicory, but repression of CiFL1 was unstable whether the post-vernalization temperature was favorable to flowering or whether it devernalized the plants. Instability of CiFL1 repression might be linked to bienniality of root chicory versus the annual life cycle of Arabidopsis. However, reactivation of AtFLC was also observed in Arabidopsis when a high temperature treatment was given straight after seed vernalization, erasing the promotive effect of cold on flowering. Cold-induced downregulation of a MADS-box floral repressor and its reactivation by high temperature thus appear as conserved features of the vernalization and devernalization responses in distant species.This article is protected by copyright. All rights reserved. Tue, 16 Apr 2013 08:15:14 GMT http://hdl.handle.net/2268/146833 Title: Analytical evaluation of virgin olive oils produced from three varieties Koroneiki , Arbequina and Arbosana grown in east of Morocco <br/> <br/>Author, co-author: Mansouri, F.; Ben Moumen, A.; Lopez, G.; Mihamou, A.; Fauconnier, Marie-Laure; Sindic, Marianne; Serghini-Caid, H.; Elamrani, A. Mon, 15 Apr 2013 09:11:01 GMT http://hdl.handle.net/2268/146832 Title: Comparative study of four safflower oils (Carthamus tinctorius) varieties grown in eastern of Morocco <br/> <br/>Author, co-author: Ben Moumen, A.; Mansouri, F.; Zraibi, L.; Abid, M.; Nabloussi, A.; Fauconnier, Marie-Laure; Sindic, Marianne; El amrani, A.; Serghini Caid, H. Mon, 15 Apr 2013 09:09:45 GMT