http://hdl.handle.net/2268/136 Search this channel search http://orbi.ulg.ac.be/simple-search http://hdl.handle.net/2268/148569 Title: The Heat Shock Response and Tissue Protection <br/> <br/>Author, co-author: Currie, R.W.; Plumier, Jean-Christophe http://hdl.handle.net/2268/148567 Title: Heat Shock Proteins ans antioxidative enzymes in myocardial protection <br/> <br/>Author, co-author: Currie, R.W.; Plumier, Jean-Christophe http://hdl.handle.net/2268/148566 Title: Brain plasticity and anti-depressant effects are versatile potential of alpha-linolenic acid to promote stroke recovery <br/> <br/>Author, co-author: Blondeau, Nicolas; Debruyne D; Piens, Marie; Nguemeni, C.; Plumier, Jean-Christophe; Marini, A. M.; Heurteaux, C. http://hdl.handle.net/2268/148564 Title: The role of Heat Shock Proteins (Hsp70 and Hsp27) in protection against ischemic injury in the myocardium and cerebral cortex <br/> <br/>Author, co-author: Plumier, Jean-Christophe http://hdl.handle.net/2268/148554 Title: Increased gastrodermal mitosis after bleaching in the sea anemone A. pallida <br/> <br/>Author, co-author: Fransolet, David; Roberty, Stéphane; Plumier, Jean-Christophe http://hdl.handle.net/2268/148550 Title: Physiologie Animale <br/> <br/>Author, co-author: Ancil, Michel; Baguet, Fernand; Charmantier, Mireille; Charmantier, Guy; Gilles, Raymond; Pequeux, André; Plumier, Jean-Christophe; Sébert, Philippe http://hdl.handle.net/2268/148477 Title: Anatomie et identification des bois <br/> <br/>Author, co-author: Jourez, Benoît <br/> <br/>Abstract: Anatomie des bois Structure anatomique des résineux et des feuillus Structure de la membrane cellulaire structure submicroscopique Anatomie du bois des essences feuillues tropicales Caractères anatomiques servant à l'identification des essences Reconnaissance microscopique du bois des essences résineuses et feuillues Duramen et duraminisation Formations anormales ( bois de compression et bois de tension) Chimie du bois Composition générale Cellulose, hémicellulose, lignine, constituants accessoires; WOOD ANATOMY Anatomical structure of softwoods and hardwoods Structure of cell wall and submicroscopical structure Wood anatomy of tropical hardwood species Anatomical features leading to the identification of wooden species Heartwood and heartwood formation Abnormal structure (compression wood and tension wood) WOOD CHEMISTRY General composition Cellulose, hemicellulose, lignine and secondary components. http://hdl.handle.net/2268/148086 Title: Lower intracellular concentration of cryoprotectants after vitrification than after slow freezing despite exposure to higher concentration of cryoprotectant solutions. <br/> <br/>Author, co-author: Vanderzwalmen, P.; Connan, Delphine; Grobet, Luc; Wirleitner, B.; Remy, Benoît; Vanderzwalmen, S.; Zech, N.; Ectors, Fabien <br/> <br/>Abstract: STUDY QUESTION: What is the intracellular concentration of cryoprotectant (ICCP) in mouse zygotes during vitrification (VIT) and slow-freezing (SLF) cryopreservation procedures? SUMMARY ANSWER: Contrary to common beliefs, it was observed that the ICCP in vitrified zygotes is lower than after SLF, although the solutions used in VIT contain higher concentrations of cryoprotectants (CPs). WHAT IS KNOWN ALREADY: To reduce the likelihood of intracellular ice crystal formation, which has detrimental effects on cell organelles and membranes, VIT was introduced as an alternative to SLF to cryopreserve embryos and gametes. Combined with high cooling and warming rates, the use of high concentrations of CPs favours an intracellular environment that supports and maintains the transition from a liquid to a solid glass-like state devoid of crystals. Although the up-to-date publications are reassuring in terms of obstetric and perinatal outcomes after VIT, a fear about exposing gametes and embryos to high amounts of CPs that exceed 3-4-fold those found in SLF was central to a debate initiated by advocates of SLF procedures. STUDY DESIGN, SIZE, DURATION: Two experimental set-ups were applied. The objective of a first study was to determine the ICCP at the end of the exposure steps to the CP solutions with our VIT protocol (n = 31). The goal of the second investigation was to compare the ICCP between VIT (n = 30) and SLF (n = 30). All experiments were performed in triplicates using mouse zygotes. The study took place at the GIGA-Research Institute of the University of Liege. PARTICIPANTS/MATERIALS, SETTING, METHODS: Cell volume is modified by changes in extracellular osmolarity. Hence, we estimated the final ICCP after the incubation steps in the VIT solutions by exposing the cells to sucrose (SUC) solutions with defined molarities. The ICCP was calculated from the SUC concentration that produced no change in cell volume, i.e. when intra- and extracellular osmolarities were equivalent. Cell volume was monitored by microscopic cinematography. ICCP was compared between SLF and VIT based on the principle that a high ICCP lowers the probability of (re)crystallization during warming but increases the probability of over-swelling of the cell due to fast inflow of water. The survival rates of mouse zygotes after SLF or VIT were compared using either (i) various warming rates or (ii) various concentrations of SUC in the warming dilution medium. MAIN RESULTS AND THE ROLE OF CHANCE: The ICCP in mouse zygotes during the VIT procedure prior to plunging them in liquid nitrogen was approximately 2.14 M, i.e. one-third of the concentration in the VIT solution. After SLF, the warming rate did not affect the zygote survival rate. In contrast, only 3/30 vitrified zygotes survived when warmed slowly but as many as 30/30 zygotes survived when warming was fast (>20 000 degrees C/min). Vitrified zygotes showed significantly higher survival rates than slow-frozen zygotes when they were placed directly in the culture medium or in solutions containing low concentrations of SUC (P < 0.01). These two experiments demonstrate a lower ICCP after VIT than after SLF. LIMITATIONS, REASONS FOR CAUTION: The results should not be directly extrapolated to other stages of development or to other species due to possible differences in membrane permeability to water and CPs. WIDER IMPLICATIONS OF THE FINDINGS: The low ICCP we observed after VIT removes the concern about high ICCP after VIT, at least in murine zygotes and helps to explain the observed efficiency and lack of toxicity of VIT. STUDY FUNDING / COMPETING INTEREST(S): The study was funded by the FNRS (National Funds for Scientific Research). The authors declare that they have no competing interests. http://hdl.handle.net/2268/145462 Title: OPTIMIZATION OF HUMAN ES CELLS (hESCs) CRYOPRESERVATION <br/> <br/>Author, co-author: Connan, Delphine; Ectors, Fabien; Grobet, Luc http://hdl.handle.net/2268/145459 Title: Lower intracellular concentration of cryoprotectants in mouse zygotes after vitrification than after slow freezing <br/> <br/>Author, co-author: Connan, Delphine; Vanderzwalmen, Pierre; Grobet, Luc; Ectors, Fabien http://hdl.handle.net/2268/145457 Title: Efficient aseptic and automatable vitrification of human Embryonic Stem Cells using bio-safe & chemically defined media <br/> <br/>Author, co-author: Connan, Delphine; Ectors, Fabien; Grobet, Luc http://hdl.handle.net/2268/145174 Title: Development and aging of the Kisspeptin-GPR54 system in the mammalian brain: what are the impacts on female reproductive function? <br/> <br/>Author, co-author: Franceschini, Isabelle; Desroziers, Elodie http://hdl.handle.net/2268/145173 Title: The intimate relationship of gonadotropin-releasing hormone neurons with the polysialylated neural cell adhesion molecule revisited across development and adult plasticity. <br/> <br/>Author, co-author: Franceschini, Isabelle; Desroziers, Elodie; Caraty, Alain; Duittoz, Anne <br/> <br/>Abstract: The neurohormone gonadotropin-releasing hormone (GnRH) is critical for all the aspects of reproductive life in vertebrates. GnRH is secreted by a small number of neurons dispersed within the preoptic-hypothalamic region. These neurons are derived from the embryonic olfactory pit. They then migrate along olfactory, vomeronasal and terminal nerves to their final destination. Classical approaches to study the regulation of GnRH secretion during the reproductive cycle have focused on the various neuronal inputs on GnRH neurons and their regulation by ovarian steroids. However, it is well known that steroids will change the microenvironment of neuronal networks and can induce plasticity and functional changes. In this review, we will focus on the intimate relationship of developing and adult GnRH neurons with the polysialylated form of neural cell adhesion molecule (PSA-NCAM), a major molecular actor in the morphogenesis and adult plasticity of the nervous system. We will first recapitulate the spatiotemporal relationship between PSA-NCAM and migrating GnRH neurons during embryogenesis of various vertebrate species and discuss its importance for GnRH neuron development as shown by various loss of function studies. In the adult, we will review the relationships between PSA-NCAM and GnRH neurons across various physiological states, and open the discussion to the use of new model systems that can help to unravel the function and mechanism of action of PSA-NCAM on GnRH neuronal network activity and GnRH release. <br/> <br/>Commentary: (c) 2010 The Authors. European Journal of Neuroscience (c) 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd. http://hdl.handle.net/2268/145158 Title: Embryonic development of kisspeptin neurones in rat. <br/> <br/>Author, co-author: Desroziers, Elodie; Droguerre, M.; Bentsen, A. H.; Robert, V.; Mikkelsen, J. D.; Caraty, A.; Tillet, Y.; Duittoz, A.; Franceschini, I. <br/> <br/>Abstract: Kisspeptins, encoded by the Kiss1 gene, play a key role in the regulation of reproductive function, although very little is known about the ontogenesis of this system. The present study aimed to determine the period of arcuate nucleus (ARC) kisspeptin cell birth and the embryonic stage and neuroanatomical sites of onset of kisspeptin immunoreactivity. Bromodeoxyuridine (BrdU) was administered to female rats at various gestational stages and double immunohistochemistry against kisspeptin and BrdU was performed on brain sections from their offspring. The period of neurogenesis of ARC kisspeptin neurones begun between embryonic day (E) 12.5 and E13.5, reached its peak at E15.5 and was not completely over at E17.5. Kiss1 mRNA was detected in mediobasal hypothalamic punches of embryos aged E14.5, E16.5, E18.5 and E22.5 by real-time reverse transcriptase-polymerase chain reaction. Accordingly, kisspeptin-immunoreactive (-IR) cells were consistently detected in the embryonic ARC from E14.5 and their number increased until E18.5 to reach approximately half the level observed in adults. Between E18.5 and E22.5, the number of kisspeptin-IR cells and hypothalamic Kiss1 expression significantly decreased, regardless of sex, and this decrease persisted until birth. Taken together, these results demonstrate that rat ARC kisspeptin neurones are born locally during an extended embryonic period and are able to synthesise kisspeptins rapidly after their birth, consistent with the hypothesis of a role during embryonic activation of the hypothalamic-hypophyseal-gonadal axis. A sex-independent decrease of kisspeptin-IR cell numbers was observed during the perinatal period, suggestive of important regulations of kisspeptin neurones around birth. <br/> <br/>Commentary: (c) 2012 The Authors. Journal of Neuroendocrinology (c) 2012 British Society for Neuroendocrinology. http://hdl.handle.net/2268/145157 Title: Kisspeptin-immunoreactivity changes in a sex- and hypothalamic-region-specific manner across rat postnatal development. <br/> <br/>Author, co-author: Desroziers, Elodie; Mikkelsen, J. D.; Duittoz, A.; Franceschini, I. <br/> <br/>Abstract: Kisspeptins are potent secretagogues of gonadotrophin-releasing hormone, playing a key role in puberty onset. These peptides are produced by distinct neuronal populations of the hypothalamus located in the rostral periventricular area of the third ventricle (RP3V) and arcuate nucleus (ARC). The present immunohistochemical study aimed to determine the spatiotemporal onset of kisspeptin-immunoreactivity (-IR) in the neonatal hypothalamus of male and female rats and to evaluate changes in kisspeptin-IR around puberty. Kisspeptin-IR cells and fibres could be detected from the day of birth in the ARC of both males and females. At this stage, only females displayed some kisspeptin-IR fibres in the RP3V. From postnatal day 7 to adulthood, males displayed lower levels of kisspeptin-IR than females in both regions. During infancy, kisspeptin-IR fibre density in the female decreased in the ARC, whereas it increased in the RP3V. A sex-independent decline in RP3V kisspeptin-IR fibre density was observed in the juvenile, followed by a peripubertal increase in RP3V and ARC kisspeptin-IR. These peripubertal increases in kisspeptin-IR occurred at different timings dependent on sex and region. In females specifically, the increase in kisspeptin-IR fibre density occurred first in the ARC and later in the RP3V under constant levels of circulating oestradiol. In conclusion, the present study highlights the expression of hypothalamic kisspeptins soon after birth, as well as the neonatal establishment of a strong and persisting sex difference in ARC kisspeptin-IR in rats. Moreover, a female-specific desynchronisation of the ARC and RP3V was observed with respect to the increase in kisspeptin-IR fibre density around puberty, which was not related to peripubertal variations in circulating oestradiol. <br/> <br/>Commentary: (c) 2012 The Authors. Journal of Neuroendocrinology (c) 2012 Blackwell Publishing Ltd. http://hdl.handle.net/2268/145156 Title: Mapping of kisspeptin fibres in the brain of the pro-oestrous rat. <br/> <br/>Author, co-author: Desroziers, Elodie; Mikkelsen, J.; Simonneaux, V.; Keller, M.; Tillet, Y.; Caraty, A.; Franceschini, I. <br/> <br/>Abstract: Kisspeptins are a family of small peptides that play a key role in the neuroendocrine regulation of the reproductive function through neural pathways that have not yet been completely identified. The present study aimed to investigate the distribution of kisspeptin neurone fibres in the female rat brain by comparing precisely the immunoreactive pattern obtained with two antibodies: one specifically directed against kisspeptin-52 (Kp-52), the longest isoform, and the other directed against kisspeptin-10 (Kp-10), whose sequence is common to all putative mature isoforms. With both antibodies, immunoreactive cell bodies were exclusively observed in the arcuate nucleus, and immunoreactive fibres were confined to the septo-preoptico-hypothalamic continuum of the brain. Fibres were observed in the preoptic area, the diagonal band of Broca, the septohypothalamic area, the anteroventral periventricular, suprachiasmatic, supraoptic, paraventricular and periventricular nuclei, the dorsal border of the ventromedian nucleus, the dorsomedial and arcuate nuclei, and the median eminence. In the latter structure, varicose fibres were mainly distributed in the internal layer and were detected to a lesser extent throughout the external layer, including around the deeper part of the infundibular recess. Most regions of immunoreactive cells and fibres matched perfectly for the two antibodies. However, fibres in the dorsolateral septum, anterior fornix, accumbens nucleus and the lateral bed nucleus of the stria terminalis were only recognised by antibody anti-Kp-10, suggesting that anti-Kp-10 may recognise a wider range of kisspeptin isoforms than anti-Kp-52 or cross-react with molecules other than kisspeptin in rat tissue. Overall, these results illustrate the variety of projection sites of kisspeptin neurones in the rat and suggest that these peptides play a role in different functions. <br/> <br/>Commentary: (c) 2010 The Authors. Journal of Neuroendocrinology (c) 2010 Blackwell Publishing Ltd. http://hdl.handle.net/2268/144687 Title: Role of Gamma Delta T cells in HPV-induced Cancer Progression <br/> <br/>Author, co-author: Van hede, Dorien; Bastin, Renaud; Francis, Floriane; Bisig, Bettina; Arrese Estrada, Jorge; Renoux, Virginie; Dortu, Estelle; Langers, Inge; Delvenne, Philippe; Vermijlen, David; Jacobs, Nathalie http://hdl.handle.net/2268/144675 Title: Role of γδ T cells in HPV-induced cancer progression <br/> <br/>Author, co-author: Van hede, Dorien; Bastin, Renaud; Francis, Floriane; Bisig, Bettina; Arrese Estrada, Jorge; Renoux, Virginie; Dortu, Estelle; Langers, Inge; Delvenne, Philippe; Jacobs, Nathalie http://hdl.handle.net/2268/144673 Title: Role of γδ T cells in the tumoural progression of HPV-associated lesions <br/> <br/>Author, co-author: Van hede, Dorien; Bastin, Renaud; Francis, Floriane; Bisig, Bettina; Arrese Estrada, Jorge; Renoux, Virginie; Dortu, Estelle; Langers, Inge; Delvenne, Philippe; Vermijlen, David; Jacobs, Nathalie http://hdl.handle.net/2268/144671 Title: Role of γδ T cells in HPV-induced cancer progression <br/> <br/>Author, co-author: Van hede, Dorien; Bastin, Renaud; Francis, Floriane; Bisig, Bettina; Arrese Estrada, Jorge; Renoux, Virginie; Dortu, Estelle; Langers, Inge; Delvenne, Philippe; Vermijlen, David; Jacobs, Nathalie