http://hdl.handle.net/2268/112 Search this channel search http://orbi.ulg.ac.be/simple-search http://hdl.handle.net/2268/150655 Title: Falsification des médicaments: mythe ou réalité ? <br/> <br/>Author, co-author: Marini Djang'Eing'A, Roland; Fillet, Marianne; Vancauwenberghe, Roy; Crommen, Jacques; Frederich, Michel; Hubert, Philippe <br/> <br/>Abstract: La santé publique est de nos jours minée par la problématique des médicaments falsifiés ou de qualité inférieure, avec plusieurs conséquences sanitaires, économiques voire professionnelles. On estime à 7% la part du marché pharmaceutique mondial que représenterait ce fléau; l’Afrique, l’Asie et de nombreux pays d'Amérique latine étant les régions les plus touchées avec plus de 30% de médicaments falsifiés. D’après l'OMS, plus de 50% des médicaments achetés à partir des sites internet illégaux sont contrefaits, annihilant très fortement les chances de succès thérapeutique. Ces médicaments viennent dans la plupart des cas des pays asiatiques et de l’Eurasie. Le trafic de faux médicaments est un crime contre l'humanité qui représente environ 50 milliards de dollars par an (10-15 % de plus que le marché de la drogue). Au travers de deux leçons, la situation de la falsification des médicaments sera présentée au grand public dans le but de le sensibiliser à ce fléau. La première leçon présentera la situation en Europe avec un accent sur la Belgique. La problématique du droit à la propriété intellectuelle et de l’encadrement législatif sera abordée, ainsi que la falsification des médicaments modernes et des phytomédicaments, ces derniers étant utilisés par plus de 40% de la population en Europe et aux Etats-Unis. Dans la seconde leçon sera abordée la situation vécue en Afrique. L’approvisionnement en médicaments de qualité par le partage de l’information sera présenté ainsi que les moyens analytiques à la disposition de ce continent pour combattre ce fléau. Des membres du Département de Pharmacie de l’Université de Liège, de l’Agence Fédérale des Médicaments et des Produits de Santé ainsi que du programme QUAMED (Quality Medicines for All) feront partager leur expérience sur cette question d’une brûlante actualité.  http://hdl.handle.net/2268/150517 Title: Is artemisinin the only antiplasmodial compound in the Artemisia annua tea infusion? An in vitro study. <br/> <br/>Author, co-author: Mouton, Julia; Jansen, Olivia; Frederich, Michel; van der Kooy, Frank <br/> <br/>Abstract: In our ongoing investigation into Artemisia annua for the treatment of malaria, we decided to study the possibility that synergism might enhance the efficacy of artemisinin. Our main objective was to test tea infusions and nonpolar extracts prepared from different A. annua varieties against Plasmodium falciparum in vitro in order to determine if synergism will increase the effectiveness of artemisinin in the samples as compared to pure artemisinin. We found that the IC50 of artemisinin in the tea and nonpolar extracts was not significantly different to the IC50 of pure artemisinin. We could show that the year and country of harvest or storage conditions did not have any influence on the activity and that it narrowly followed the concentration of artemisinin in all the extracts. In conclusion, based on these in vitro results, artemisinin seems to be the only active antiplasmodial compound in A. annua. <br/> <br/>Commentary: Georg Thieme Verlag KG Stuttgart . New York. http://hdl.handle.net/2268/150516 Title: Some natural products from aerial parts of Scrophularia imerethica <br/> <br/>Author, co-author: Getia, M; Mshvildadze, V; Dekanosidze, G; Wauters, Jean-Noël; Tits, Monique; Frederich, Michel http://hdl.handle.net/2268/150502 Title: Développement de vecteurs polymériques de type polyplexes à base de nouveaux polymères cationiques biodégradables de type polycarbonates et de siRNA dirigés spécifiquement contre l’histone déacétylase 7 <br/> <br/>Author, co-author: Frère, Antoine; Piel, Géraldine http://hdl.handle.net/2268/150501 Title: Développement de vecteurs polymériques de type polyplexes à base de nouveaux polymères cationiques biodégradables de type polycarbonates et de siRNA dirigés spécifiquement contre l’histone déacétylase 7 <br/> <br/>Author, co-author: Frère, Antoine; Piel, Géraldine http://hdl.handle.net/2268/150494 Title: EVALUATION OF A NEW BIOCOMPATIBLE POLY(N-(MORPHOLINO ETHYL METHACRYLATE)-BASED COPOLYMER FOR THE DELIVERY OF Ruthenium oligonucleotides, targeting HPV16 E6 oncogene <br/> <br/>Author, co-author: Reschner, Anca; Shim, Yong Ho; Dubois, Philippe; Evrard, Brigitte; DELVENNE, Philippe; Marcelis, Lionel; Moucheron, Cécile; Kirsch Demmesmacher, Andrée; Defrancq, Eric; Raes, Martine; Collard, Laurence; Piel, Géraldine http://hdl.handle.net/2268/150442 Title: Optimization of a Pharmaceutical Tablet Formulation: Screening Experiments using Vibrational Spectroscopy as PAT Tool <br/> <br/>Author, co-author: Chavez, Pierre-François; Netchacovitch, Lauranne; Sacre, Pierre-Yves; De Bleye, Charlotte; Pestieau, Aude; Krier, Fabrice; Mantanus, Jérôme; Motte, Henri; Schubert, Martin; Evrard, Brigitte; Hubert, Philippe; Ziemons, Eric http://hdl.handle.net/2268/150336 Title: Usefulness of Information Criteria for the Selection of Calibration Curves <br/> <br/>Author, co-author: Rozet, Eric; Ziemons, Eric; Marini Djang'Eing'A, Roland; Hubert, Philippe <br/> <br/>Abstract: The reliability of analytical results obtained with quantitative analytical methods is highly dependant upon the selection of the adequate model used as calibration curve. To select the adequate response function or model the most used and known parameter is the determination coefficient R². However it is well known that it suffers many inconvenient, such as leading to overfitting the data. A solution proposed is to use the adjusted determination coefficient R²adj that aims at reducing this problem. However there is another family of criteria that exists to allow the selection of an adequate model: the information criteria AIC, AICc and BIC. These criteria have rarely been used in analytical chemistry to select the adequate calibration curve. This works aims at assessing the performance of the statistical information criteria as well as R² and R²adj for the selection of an adequate calibration curve. They are applied to several analytical methods covering liquid chromatographic methods as well as electrophoretic ones involved in the analysis of active substances in biological fluids or aimed at quantifying impurities in drug substances. In addition, Monte-Carlo simulations are performed to assess the efficacy of these statistical criteria to select the adequate calibration curve. http://hdl.handle.net/2268/150331 Title: Neurobiologie de l'addiction <br/> <br/>Author, co-author: Scuvée, Jacqueline http://hdl.handle.net/2268/150267 Title: Qualitative Approaches to Monitor Pharmaceutical Blending Processes using In-line Near-Infrared Spectroscopy <br/> <br/>Author, co-author: Chavez, Pierre-François; Sacre, Pierre-Yves; De Bleye, Charlotte; Netchacovitch, Lauranne; Motte, Henri; Hubert, Philippe; Ziemons, Eric http://hdl.handle.net/2268/150230 Title: Principes du traitement pharmacologique de l'addiction <br/> <br/>Author, co-author: Scuvée, Jacqueline http://hdl.handle.net/2268/150147 Title: A HYDROGEN BOND INFLUENCES THE 5-HT1A/D4 SELECTIVITY OF WAY-100635 ANALOGUES: AN IN SILICO APPROACH <br/> <br/>Author, co-author: Dilly, Sébastien; Liégeois, Jean-François <br/> <br/>Abstract: WAY-100635 is widely used in vitro and in vivo as an antagonist of 5-HT1A receptors. In terms of pharmacological tools and pharmacological investigations, the ideal reference molecule would be highly selective for its target over other related and non-related targets. However WAY-100635 displays affinity for and activity at D4 dopamine receptors, and that "off-target" activity confounds its use in pharmacological studies, particularly when both receptors are present. In this context, we carried out various chemical modifications of the WAY-100635 structure in order to improve its 5-HT1A versus D4 selectivity. An important increase of selectivity was obtained when the basic side chain of WAY-100635 was replaced by a 4-phenylpiperazine or a 4-phenyl-1,2,3,6-tetrahydropyridine moiety. In contrast, the introduction of nitrogen atoms in the acyl group decreased the selectivity by reducing the affinity for 5-HT1A receptors, on the one hand, and enhancing the affinity for D4 receptors on the other hand. In order to explain the reduced 5-HT1A/D4 selectivity of aza-derivatives, the binding modes of the compounds were explored by docking analysis on homology models of the two receptors. It appears that the formation of an additional hydrogen bond within D4 receptors could be the key of the decreased selectivity. These results will be very helpful for developing molecules with an improved 5-HT1A/D4 selectivity. http://hdl.handle.net/2268/149773 Title: Age-related Macular Degeneration (AMD): from Metabolomics approach to the inhibition of PDH Kinase as a new therapeutic target <br/> <br/>Author, co-author: Arslan, Deniz; Dilly, Sébastien; LAMBERT, Vincent; Hansen, Sylvain; RAKIC, Jean-Marie; Noël, Agnès; Govaerts, Bernadette; Pirotte, Bernard; De Tullio, Pascal http://hdl.handle.net/2268/149323 Title: NON-ANTHOCYANIN POLYPHENOLS QUANTIFICATION IN EUTERPE OLERACEA FRUITS BY A UHPLC−LTQ-ORBITRAP MS METHOD <br/> <br/>Author, co-author: Dias, Aecio; Rozet, Eric; Chataigné, G; Margalho, L; Larondelle, Y; Hubert, Philippe; Roger, H; Quetin-Leclercq, J <br/> <br/>Abstract: High antioxidant and anti-inflammatory activities have been observed from non-anthocyanin polyphenols of E. oleracea fruits [1-2]. The aim of this work was to quantify major non-anthocyanin polyphenols by an accurate UHPLC−LTQ-Orbitrap MS method. Fruits were harvested in Pará state (Brazil), processed to pulp and lyophilised. 0.5g of dry pulp powder was defatted by sonication with petroleum ether. The residue was then extracted five times with 5mL MeOH each time for 30 min (optimized conditions giving recovery rates > 90%). The extract was evaporated to dryness with a RapidVap® evaporator at 35°C. Solubilization of the dried extract was realised using 40% MeOH. For the UHPLC quantification, a HSS C18 column (1.8µm) was used with a gradient elution of MeOH and H2O both with 0.1% HCOOH and the ionisation source (ESI) was operated in NI mode. 26 compounds were identified, among them 7 identified for the first time in this fruit. Total error and accuracy profiles were used as validation criteria. Calibration in the matrix was found to be more accurate than calibration without matrix. Trueness, repeatability, intermediate precision, selectivity, response function, linearity and LOD/LOQ for 12 non-anthocyanin phenolic compounds were evaluated and the quantification method validated. [1] J. Kang et al., Food Chem. 122 (2010) 610–617. [2] J. Kang et al., Food Chem. 128 (2011) 152–157. http://hdl.handle.net/2268/149207 Title: MEASURING VARIABILITY SOURCES IN NMR METABOLOMIC STUDIES <br/> <br/>Author, co-author: Rozet, Eric; De Tullio, Pascal; Hubert, Philippe; Govaerts, B <br/> <br/>Abstract: Due to the huge amount of information available in NMR spectra obtained from the analysis of metabolomic experiments, multivariate analysis such as Principal Component Analysis (PCA) are required to understand the influence of treatments over the metabolites [1]. However, many experiments in metabolomics studies have more complexes variability structures than simply comparing several treatments: they may include time effects, biological effects such as diet or hormonal status, and other blocking factors or variability sources: samples stability, age of the individuals, pH of a buffer, days of acquisition, and so on. Metabolomic data analysis needs to extract from the spectral data matrix the variations linked to a change indicated in the factor of interest. However other sources of variability may impair this objective. This stresses the importance to discover the sources of variability of the spectral metabolomic data using appropriate methodology. Classically, to analyze such data analysis of variance (ANOVA) or multivariate ANOVA (MANOVA) [2] is used. However direct application of these methodologies to NMR spectra obtained from structured metabolomics studies is inappropriate or impossible. More complex data analyses methodologies are required to understand the importance of the various factors implied in the experiments and to provide a measure of their variance components. Three related methodologies have been proposed to achieve this: ASCA [3], ANOVA-PCA [4] and AComDim [5]. The ASCA and ANOVA-PCA methodologies combine first an analysis of variance step (ANOVA) and then a PCA step. The AComDim one adds to the output of the ANOVA-PCA step a multi-block analysis. In this presentation, the usefulness and applicability of these advanced techniques to data analysis of NMR metabolomic spectra are provided to highlight the increase of knowledge gained and the estimation of main sources of variability arising in an experimental setup. Two NMR databases will be used [6]. The first one concerns human serum analyzed by 1H-NMR where three random factors are present: day of measurement (3 days), sample (2 samples per individual) and replication of analyses as well as two fixed controlled factors, time of measurement after thawing (2 times) and two protein suppression methods for the spectral pre-treatment. The second database is about the 1H-NMR analyses of rats’ urine where two different concentrations of citrate and of hippurate were deliberately added and three other sources of variability are present: urine pool diluted or not diluted, repetitions of analyses, days of analyses (three days), as well as two different spectral pre-treatment procedures. http://hdl.handle.net/2268/149132 Title: Drug related problems and interventions of pharmacists on prescribed medicines in Belgium <br/> <br/>Author, co-author: De Wulf, Isabelle; Boussery, Koen; De Vriese, Carine; De Meyer, Guido; Foulon, Veerle; Lacour, Valérie; Mehuys, Els; Steurbaut, Stephane; Van Hees, Thierry <br/> <br/>Abstract: Aim of project/study (1) To study the frequency and nature of drug related problems (DRP) detected by community pharmacists and internship students when dispensing prescribed medicines. (2) To investigate the nature and frequency of interventions by pharmacists. (3) To study whether there is a difference between DRP detection at the moment of dispensing versus in a quiet setting. http://hdl.handle.net/2268/148735 Title: Dosages quantitaifs de principes actifs dans les formes solides par spectroscopie proche infrarouge: Application pratique au test d'uniformité de teneur des comprimés <br/> <br/>Author, co-author: Ginot, Yves-Michel; Bernard-Moulin, Patrick; Boiret, Mathieu; Cinier, Régis; Despagne, Frédéric; Dubost, Jean-Pierre; Frances, Laurent; Le Page, Marc; Michelet, Alexandre; Ziemons, Eric <br/> <br/>Abstract: L'augmentation du nombre de dosages à réaliser dans l'industrie pharmaceutique conduit à rechercher des méthodes alternatives. La spectroscopie proche infrarouge répond précisément à cette demande en permettant des dosages rapides, non destructifs et respectueux de l'environnement. Le texte publié dans SFSTP Pharma Pratiques en 2010 énonçait la méthodologie à suivre pour développer une technique de dosage de substance active dans une formulation solide de type comprimé par spectroscopie proche infrarouge. Il est apparu intéressant d'illustrer et de compléter les différentes étapes de cette méthodologies au travers d'exemples. http://hdl.handle.net/2268/148684 Title: IDENTIFICATION AND DETERMINATION OF ALKALOIDS IN FUMARIA SPECIES FROM ROMANIA <br/> <br/>Author, co-author: Paltinean, Ramona; Toju, A; Wauters, Jean-Noël; Frederich, Michel; Tits, Monique; Angenot, Luc; Tamas, M; Crisan, G http://hdl.handle.net/2268/148535 Title: Les génériques d’antibiotiques : une fausse bonne idée ? <br/> <br/>Author, co-author: Van Hees, Thierry http://hdl.handle.net/2268/148303 Title: COMPARATIVE MORPHOLOGICAL STUDIES ON SOME SPECIES OF THE GENUS FUMARIA <br/> <br/>Author, co-author: Paltinean, Ramona; Wauters, Jean-Noël; Tits, Monique; Frederich, Michel; Angenot, Luc; Mircea, Tamas; Crisan, Gianina