http://hdl.handle.net/2268/108 Search this channel search http://orbi.ulg.ac.be/simple-search http://hdl.handle.net/2268/147896 Title: Microbiological diagnosis of infectious keratitis <br/> <br/>Author, co-author: MELIN, Pierrette http://hdl.handle.net/2268/144192 Title: Epithelial ingrowth cells after LASIK/ALTK (automated lamellar therapeutic keratoplasty): are they corneal epithelial stem cells? <br/> <br/>Author, co-author: Nicolas, Michael; Abouzeid, Hana; DEPREZ, Manuel; Hafezi, Farhad; Munier, Francis L.; Varga, Zsolt; Majo, Francois http://hdl.handle.net/2268/141884 Title: Place de l'ophtalmologie en unité néonatale : actualités <br/> <br/>Author, co-author: VIELLEVOYE, Renaud; MILET, Ariane http://hdl.handle.net/2268/133393 Title: Assessment of new-generation glistening-free hydrophobic acrylic intraocular lens material <br/> <br/>Author, co-author: Pagnoulle, Christophe; Bozukova, Dimitriya; Gobin, Laure; Bertrand, Virginie; Gillet, Marie-Claire <br/> <br/>Abstract: To determine the hydrophobic, antiglistening, and bioadhesiveness properties of a new polymer, GF rawmaterial, and to determine the suitability of thismaterial for use in intraocular lenses (IOLs). http://hdl.handle.net/2268/116092 Title: Les paralysies du nerf moteur oculaire commun chez l'enfant: a propos d'une observation de tumeur germinale hypophysaire. <br/> <br/>Author, co-author: Kalenga, Mbu; COLLIGNON, Nathalie; ANDRIS, Cécile; Deprez, Manuel; OTTO, Bernard <br/> <br/>Abstract: PURPOSE: Third cranial nerve palsies are unfrequent in childhood and adolescence and are most often congenital. The association of sellar germ cell tumor and ophthalmoplegia is considered as being very rare at this age. CASE REPORT: A 11-year-old young girl was examined in emergency with a third left cranial nerve partial palsy associated with one- year duration history of hypopituitarism with insipid diabetes and growth retardation. Cerebral IRM revealed a tumor of the pituitary gland. In histopathological examination of pituitary gland biopsies, lesions were compatibles with a sellar germ cell tumor. CONCLUSION: Although they are most often of a congenital nature, third cranial nerve palsies in childhood may be secondary to other causes that should be always taken in mind. When they are secondary to a sellar tumor and according to the clinical presentation and the IRM, the histopahological examination of biopsies is mandatory to have a precise diagnosis. http://hdl.handle.net/2268/113683 Title: Clinicopathologic and molecular analysis of a choroidal pigmented schwannoma in the context of a PTEN hamartoma tumor syndrome. <br/> <br/>Author, co-author: Venturini, Giulia; Moulin, Alexandre P.; DEPREZ, Manuel; Uffer, Sylvie; Bottani, Armand; Zografos, Leonidas; Rivolta, Carlo <br/> <br/>Abstract: PURPOSE: To report the first case of choroidal schwannoma in a patient affected by PTEN hamartoma tumor syndrome (PHTS) and investigate the molecular involvement of the phosphatase and tensin homolog (PTEN) and neurofibromin 2 (NF2) genes in this rare intraocular tumor. DESIGN: Observational case report. PARTICIPANT: A 10-year-old girl diagnosed with PHTS. METHODS: The enucleated specimen underwent histologic, immunohistochemical, and transmission electronic microscopy. The expression of PTEN and NF2 and their protein products were evaluated by reverse transcription-polymerase chain reaction and immunohistochemistry. Somatic mutations of PTEN and NF2, as well as allelic loss, were investigated by direct sequencing of DNA extracted from the tumor. PTEN epigenetic silencing was investigated by pyrosequencing. MAIN OUTCOME MEASURES: Histopathologic and molecular characterization of a choroidal pigmented schwannoma. RESULTS: Histopathologic, immunohistochemical, and electron microscopic analysis demonstrated features consistent with a pigmented cellular schwannoma of the choroid. We found no loss of heterozygosity at the genomic level for the PTEN germline mutation and no promoter hypermethylation or other somatic intragenic mutations. However, we observed an approximate 40% reduction of PTEN expression at both the mRNA and the protein level, indicating that the tumor was nonetheless functionally deficient for PTEN. Although DNA sequencing of NF2 failed to identify any pathologic variants, its expression was abolished within the tumor. CONCLUSIONS: We report the first description of a pigmented choroidal schwannoma in the context of a PHTS. This rare tumor showed a unique combination of reduction of PTEN and absence of NF2 expression. <br/> <br/>Commentary: Copyright (c) 2012 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved. http://hdl.handle.net/2268/101460 Title: La Toxine Botulinique : applications cliniques en ophtalmologie. <br/> <br/>Author, co-author: MARTIN, Didier; ANDRIS, Cécile http://hdl.handle.net/2268/37013 Title: Infections oculaires: prélèvements et examen microbiologiques <br/> <br/>Author, co-author: MELIN, Pierrette http://hdl.handle.net/2268/77399 Title: Prélèvement et rôle du laboratoire <br/> <br/>Author, co-author: MELIN, Pierrette http://hdl.handle.net/2268/82458 Title: Infections oculaires traumatiques et iatrogènes: prélèvements et rôle du laboratoire <br/> <br/>Author, co-author: Melin, Pierrette <br/> <br/>Abstract: Ocular inflammations may be due to a variety of diseases, and microorganisms play a major role in both acute and chronic eye diseases. Treatment of serious infections needs a good microbiological diagnostic. The indications and techniques for investigations are determined by the site of infection, severity of the process, and knowledge of the likely responsible organisms. Immediate inoculation of specimens on culture media in the examining room is often recommended. This article describes organisms associated with ocular infections, and is designed to assists ophthalmologists in the collection, processing and transport of specimens. http://hdl.handle.net/2268/82456 Title: A propos d'un cas de kératite a Mycobacterium chelonae <br/> <br/>Author, co-author: Duchesne, Bernard; Marechal-Courtois; Melin, Pierrette <br/> <br/>Abstract: The authors presents one case of primitive corneal mycobacterium keratitis after corneal graft. This rare form of keratitis is analysed in the field of mycobacterial infections who's nowadays more developed in our industrialised countries. The description of the case point out the multiresistance of this mycobacterium and describe the different treatments. http://hdl.handle.net/2268/82039 Title: Cas d'un ulcère à Exophiala dermatitidis <br/> <br/>Author, co-author: Gérard, C.; Duchesne, Bernard; Hayette, Marie-Pierre; Lavalleye, B.; Marechal-Courtois, C. <br/> <br/>Abstract: The authors present one case of corneal Exophiala dermatitidis ulceration. This case is analyzed in the field of corneal mycosis infection. Classification, rate, clinical history, biomicroscopy aspects will be discussed. Technical samples will be described. Anatomopathologic slides will be discussed. Finally the medical or surgical treatment will be considered.; Les auteurs présentent un cas d'ulcère cornéen à Exophiala dermatitidis. Ce cas permet d'envisager la classification des mycoses cornéennes ainsi que leur fréquences. L'histoire clinique et l'aspect biomicroscopique permettent de mettre en évidence les critères diagnostiques des infections mycotiques. L'intérêt et la techique des prélèvements seront discutés. l'analyse anatomopathologique après greffe de cornée confirme le diagnostic et nous rappelle la place essentielle de cet examen trop souvent négligé. L'attitude thérapeutique tant médicale que chirurgicale sera discutée. http://hdl.handle.net/2268/81015 Title: Bone marrow-derived mesenchymal cells and MMP13 contribute to experimental choroidal neovascularization. <br/> <br/>Author, co-author: Lecomte, Julie; Louis, Krystel; Detry, Benoît; Blacher, Silvia; Lambert, Vincent; Bekaert, Sandrine; Munaut, Carine; Paupert, Jenny; Blaise, Pierre; Foidart, Jean-Michel; Rakic, Jean-Marie; Krane, S. M.; Noël, Agnès <br/> <br/>Abstract: In this study, we evaluate the potential involvement of collagenase-3 (MMP13), a matrix metalloproteinase (MMP) family member, in the exudative form of age-related macular degeneration characterized by a neovascularisation into the choroid. RT-PCR analysis revealed that human neovascular membranes issued from patients with AMD expressed high levels of Mmp13. The contribution of MMP13 in choroidal neovascularization (CNV) formation was explored by using a murine model of laser-induced CNV and applying it to wild-type mice (WT) and Mmp13-deficient mice (Mmp13 ( -/- ) mice). Angiogenic and inflammatory reactions were explored by immunohistochemistry. The implication of bone marrow (BM)-derived cells was determined by BM engraftment into irradiated mice and by injecting mesenchymal stem cells (MSC) isolated from WT BM. The deficiency of Mmp13 impaired CNV formation which was fully restored by WT BM engraftment and partially rescued by several injections of WT MSC. The present study sheds light on a novel function of MMP13 during BM-dependent choroidal vascularization and provides evidence for a role for MSC in the pathogenesis of CNV. http://hdl.handle.net/2268/64378 Title: A propos d'un cas de kératite à Acanthamoeba <br/> <br/>Author, co-author: Carrette, S.; Marechal-Courtois, C.; Hernandez, J.; Hayette, Marie-Pierre; Deprez, Manuel; Duchesne, Bernard <br/> <br/>Abstract: CLINICAL REPORT: A clinical report of a contact lenses wearer with Acanthamoeba keratitis pointed out the diagnosis problem. The medical treatment is needed previously to any surgery. Finally the patient underwent enucleation. DISCUSSION: The authors are considering the microbiological aspects and laboratory techniques are described. CONCLUSION: For this very severe but hopefully rare pathology, the sooner the treatment the best. A therapeutic approach is described. http://hdl.handle.net/2268/64373 Title: Clinicopathological features of eyelid skin tumors. A retrospective study of 5504 cases and review of literature. <br/> <br/>Author, co-author: Deprez, Manuel; Uffer, Sylvie <br/> <br/>Abstract: Eyelid tumors are the most common neoplasm in daily ophthalmology practice and encompass a wide variety of benign and malignant tumors. In this retrospective study, we report the clinical and histological features of 5504 eyelid skin tumors diagnosed at the Laboratory of Ophthalmopathology of the Hopital Ophtalmique Jules Gonin, Lausanne, Switzerland, between January 1989 and December 2007. Benign tumors largely predominated over malignant ones, representing 84% of cases in this series, and the 5 most frequent subtypes were squamous cell papilloma (26%), seborrheic keratosis (21%), melanocytic nevus (20%), hidrocystoma (8%), and xanthoma/xanthelasma (6%). Basal cell carcinoma was the most frequent malignant tumor (86%), followed by squamous cell carcinoma (7%) and sebaceous carcinoma (3%). For several tumor subtypes, there was a poor correlation between clinical and histological diagnosis, stressing the numerous pitfalls in the diagnosis of eyelid tumors. We further discuss our results with reference to previously published series. http://hdl.handle.net/2268/30882 Title: Rôle des protéinases et de leurs inhibiteurs dans les formes associées à une néovascularisation anormale sous-rétinienne. Caractérisation par l’étude de modèles animaux transgéniques et développements thérapeutiques. <br/> <br/>Author, co-author: Lambert, Vincent <br/> <br/>Abstract: La dégénérescence maculaire liée à l'âge (DMLA) est la principale cause de perte visuelle chez les personnes de plus de 70 ans dans le monde occidental. Cette cécité résulte de la perte de fonction de la macula (au centre de la rétine) qui est responsable de la vision centrale. Cette dégénérescence réprésente un handicap social indéniable et une perte de liberté pour ces personnes atteintes, car elle affecte des activités telles que la conduite, la lecture ou tout simplement la reconnaissance d’un visage. La pathogénie de cette affection est complexe et peu comprise. La forme la plus grave de cette maladie est associée à une poussée vasculaire (angiogenèse) vers l'espace sous-rétinien qui entraîne une fibrose avec perte des photorécepteurs. La plupart des lésions ne sont pas curables par photocoagulation laser et les stratégies thérapeutiques actuelles sont insuffisantes. Nous avons étudié, dans notre laboratoire, les mécanismes moléculaires de l’angiogenèse. Ce processus invasif requiert la digestion de la matrice extracellulaire avant la prolifération et la migration des capillaires. Deux systèmes enzymatiques sont principalement impliqués dans la digestion tissulaire : le système plasminogène/plasmine et les métalloprotéinases matricielles (MMPs). 1) Le système protéolytique activateur du plasminogène/plasmine : Afin d'évaluer la pertinence biologique de ce système dans l'angiogenèse sous-rétinienne, nous avons mis au point un modèle de néovascularisation choroidienne à l'aide d'un impact laser à l'argon chez des souris déficientes pour une série de gènes fondamentaux de ce système, comparées à des souris normales. Nos observations mettent en évidence le rôle essentiel du PAI-1 dans la néovascularisation sous-rétinienne et contribuent à expliquer l'aspect multifonctionnel du PAI-1 dans l'angiogenèse. 2) Les métalloprotéinases matricielles (MMPs) : Les métalloprotéinases matricielles (MMPs) participent au remodelage normal tissulaire et sont impliquées dans un large éventail de phénomènes physiologiques et pathologiques, tels que l'invasion cellulaire tumorale, la cicatrisation et l'angiogenèse. Nous avons appliqué notre modèle expérimental à des souris déficientes pour deux types de MMP (MMP-2 et MMP-9). La déficience en MMP-2 et -9 inhibe la néovascularisation sous-rétinienne induite par laser, soulignant l’importance de cette enzyme in vivo. Ces études laissent entrevoir des perspectives thérapeutiques dans la lutte contre la DMLA par l’utilisation d’inhibiteur spécifiques des ces MMPs. En conclusion, ces différents aspects de notre étude ouvrent des perpectives de développements thérapeutiques. Ils démontrent l’importance des MMP-2, MMP-9 et de PAI-1 dans la néovascularisation sous-rétinienne. L’inhibition spécifique de ces enzymes laisse entrevoir des espoirs dans le traitement curatif de la dégénérescence maculaire liée à l’âge. Malheureusement de grandes lacunes existent encore quand aux perturbations métaboliques à l’origine de la DMLA, ce qui est l’objet de cette étude. http://hdl.handle.net/2268/26567 Title: Influence of Plasminogen Activator Inhibitor Type 1 on Choroidal Neovascularization <br/> <br/>Author, co-author: Lambert, Vincent; Munaut, Carine; Frankenne, F.; Bajou, Khalid; Gerard, R.; Carmeliet, P.; Defresne, Marie-Paule; Foidart, Jean-Michel; Rakic, Jean-Marie; Noël, Agnès <br/> <br/>Abstract: High levels of the plasminogen activators, but also their inhibitor, plasminogen activator inhibitor 1 (PAI-1), have been documented in neovascularization of severe ocular pathologies such as diabetic retinopathy or age-related macular degeneration (AMD). AMD is the primary cause of irreversible photoreceptors loss, and current therapies are limited. PAI-1 has recently been shown to be essential for tumoral angiogenesis. We report here that deficient PAI-1 expression in mice prevented the development of subretinal choroidal angiogenesis induced by laser photocoagulation. When systemic and local PAI-1 expression was achieved by intravenous injection of a replication-defective adenoviral vector expressing human PAI-1 cDNA, the wild-type pattern of choroidal angiogenesis was restored. These observations demonstrate the proangiogenic activity of PAI-1 not only in tumoral models, but also in choroidal experimental neovascularization sharing similarities with human AMD. They identify therefore PAI-1 as a potential target for therapeutic ocular anti-angiogenic strategies. http://hdl.handle.net/2268/26536 Title: Matrix metalloproteinase-9 contributes to choroidal neovascularization <br/> <br/>Author, co-author: Lambert, Vincent; Munaut, Carine; Jost, M.; Werb, Z.; Foidart, Jean-Michel; Rakic, Jean-Marie; Noël, Agnès <br/> <br/>Abstract: Age-related macular degeneration (AMD) is the primary cause of irreversible photoreceptors loss in adult patients and current therapies are limited. Increased levels of matrix metalloproteinases (MMPs) have been documented in neovascularization of severe ocular pathologies such as AMD and proliferative diabetic retinopathy. We report here that MMP-9 (gelatinase B) expression is induced and temporally regulated in the course of experimental choroidal neovascularization. We used transgenic mice expressing beta-galactosidase reporter gene under the dependence of MMP-9 promoter and RT-PCR analysis on choroidal neovascular structures microdissected from serial sections by laser pressure catapulting to show that MMP-9 expression is up-regulated concomitantly with the appearance of inflammatory cells in the subretinal lesion. In mice deficient in MMP-9 expression the development of choroidal neovascularization induced by laser photocoagulation still occurred, but at a reduced level. http://hdl.handle.net/2268/25015 Title: La rétinopathie du prématuré <br/> <br/>Author, co-author: Battisti, Oreste <br/> <br/>Abstract: cours sur la rétinopathie du prématuré http://hdl.handle.net/2268/12217 Title: Molecular targeting of antiangiogenic factor 16K hPRL inhibits oxygen-induced retinopathy in mice <br/> <br/>Author, co-author: Pan, H.; Nguyen, Ngoc-Quynh-Nhu; Yoshida, H.; Bentzien, F.; Shaw, L. C.; Rentier-Delrue, Françoise; Martial, Joseph; Weiner, R.; Struman, Ingrid; Grant, M. B. <br/> <br/>Abstract: PURPOSE. To examine the ability and mechanism of the 16 kDa N-terminal fragment of human prolactin (16K hPRL) in the inhibition of abnormal retinal neovascularization. METHODS. The 16K hPRL-encoding sequence was inserted into an adenoviral vector (16K-Ad). Western blot analysis verified the expression of 16K hPRL and inhibition of proliferation, confirming functional activity of the 16K hPRL in virus-infected adult bovine aortic endothelial (ABAE) cells. 16K hPRL inhibited retinal neovascularization in a mouse model of oxygen-induced retinopathy. The ability of recombinant 16K hPRL expressed in E. coli (r16K hPRL) was compared to that of endostatin in inducing apoptosis of cultured human retinal endothelial cells (HREC). RESULTS. 16K was expressed in virus-infected ABAE cells and resulted in a dose-dependent inhibition of cell proliferation. Eyes injected with 16K-Ad showed a reduction in preretinal neovascularization of 82.3 +/- 9.3% (P < 0.00001) when compared to uninjected controls. r16K hPRL was 100 times more potent than endostatin in inducing apoptosis in HRECs. CONCLUSIONS. Intravitreal administration of 16K hPRL inhibited neovascularization in the mouse model of oxygen-induced retinopathy. 16K hPRL stimulated apoptosis in HRECs and inhibited cell proliferation in ABAE cells. These results suggested a potential therapeutic role for 16K hPRL in the treatment of proliferative retinopathies.