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See detailMulti-allergens detection by flow cytometric immunoassay
Otto; Planque; Lamote et al

Poster (2015, November)

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See detailIntra-cultivar potential of Desmanthus spp. as a greenhouse gas mitigation strategy for tropical livestock pastoral systems
Vandermeulen, Sophie ULg; Ramírez-Restrepo, Carlos Alberto; Singh, Sultan et al

Poster (2015, November)

Improved agricultural efficiency and reduction in the impacts of tropical livestock farming on habitat degradation require global approaches that enhance ruminant farming functionality in terms of feed ... [more ▼]

Improved agricultural efficiency and reduction in the impacts of tropical livestock farming on habitat degradation require global approaches that enhance ruminant farming functionality in terms of feed use efficiency, emissions and food security. This study evaluated the in vitro mitigation potential of the prostrate to erect, herbaceous Desmanthus spp. pasture legume adapted to semiarid clay soil land types in northern Australia. D. bicornutus, D. leptophyllus and D. virgatus were seasonally harvested from commercial plots by Agrimix Pty. Ltd. Samples of the legumes and the control Rhodes grass (Chloris gayana) using ruminal fluid from grazing Brahman (Bos indicus) steers were incubated in vitro (Ankom RF1 Technology) for 24, 48 and 72 h. Overall, the in vitro organic matter degradability (OMD) and methane production between Desmanthus species differed (P < 0.001). Compared to the control (0.656 ± 0.027 proportion of total OM) at 48 h of incubation, D. leptophyllus showed lower OMD (0.479 ± 0.016), while D. bicornutus (0.688 ± 0.016) and D. virgatus (0.619 ± 0.015) were different from each other, but similar to the control. Methane production (ml/g OM) was 15.7 ± 1.54, 3.7 ± 0.89, 12.0 ± 0.95 and 11.7 ± 0.95, respectively. It is suggested that the impact of these attributes may benefit household farmers in developing economies to expand productivity, improve livelihoods and meet the growing food consumption. Further analyses of the intra-cultivar characteristics of Desmanthus spp. will complement the design of sustainable and efficient interventions across tropical pastoral feeding systems, with a particular emphasis on large-scale grazing operations. [less ▲]

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See detailTwo Types of Aurora on Mars as Observed by MAVEN's Imaging UltraViolet Spectrograph
Schneider; Deighan; Jain et al

Poster (2015, November)

Detailed reference viewed: 17 (2 ULg)
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See detailEvaluation of the contamination of the soil and forage by heavy metals in the pastureland of the north west of Tunisia
Riahi, Thameur; Colinet, Gilles ULg; Said, S et al

Poster (2015, November)

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See detailInnovative methods for the determination of the taxonomic origin of processed animal proteins in feed.
Veys, Pascal; Ngo Njembe, M.; Lecrenier, Marie-Caroline ULg et al

Poster (2015, November)

The use of animal by-products in feed depends on their nature defined by the type of tissue or body parts and the species of origin. Currently, the detection of unauthorised processed animal proteins ... [more ▼]

The use of animal by-products in feed depends on their nature defined by the type of tissue or body parts and the species of origin. Currently, the detection of unauthorised processed animal proteins (PAPs) is based on light microscopy and PCR methods. Light microscopy identifies structures on the basis of their morphology and enables identification of particles (such as bones, cartilages, muscle fibres,…) while PCR is able to detect and identify the presence of specific animal DNA in feed. Nevertheless, for some scenarios, even combined, these methods do not succeed in determining the taxonomic origin of the PAPs. A typical example is that of an aquafeed containing authorised porcine PAP together with dairy products: the analysis will conclude of the potential presence of ruminant PAP. Therefore, there is a need for developing methods allowing a taxonomic characterisation of visual structures such as bones fragments and muscle fibres. For the characterisation of bones, NIRM has yet demonstrated its potential. However the limitation of NIRM is when the presence of bones is reduced or absent. This study investigated the potential of NIRM for the determination of the taxonomic origin of muscle fibres. The NIRM was experimented on 2 porcine PAPs vs. 6 ruminant PAPs and 7 fishmeals all of industrial origin. Results showed that NIRM allows differentiating muscle fibres from different taxonomic origins: fish, ruminant and pig. In addition to this taxonomic classification, results also reveal differences inside taxonomic clusters of PAPs (e.g among different ruminant PAPs and porcine PAPs). The results obtained on this type of meals are promising and offer new perspectives. Tests on adulterated feeds need to be performed by NIRM prior to validation. [less ▲]

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See detailHemipteran - host plant interactions: focus on some model insect saliva
Serteyn, Laurent ULg; Moreau, Sophie; Delatour, Anaïs et al

Poster (2015, November)

Insect salivary components play important roles in plant-insect interactions, stimulating the plant defence or promoting infestation by manipulating plant physiology. Indeed, a variety of enzymes and ... [more ▼]

Insect salivary components play important roles in plant-insect interactions, stimulating the plant defence or promoting infestation by manipulating plant physiology. Indeed, a variety of enzymes and organic components in saliva of herbivory insects can induce series of biochemical responses in damaged plants. In our lab, several studies have been screening proteins in different aphid species saliva. The purpose of our current and future works is to widen that field of study to other piercing-sucking pests and host plants models. Three models will be compared: the pea aphid (Acyrtosiphon pisum Harris), the invasive Asian brown marmorated stink bug (Halyomorpha halys Stål) and the invasive South American green vegetable stink bug (Nezara viridula L.). A preliminary study showed differential expressions in bacterial proteins in salivary glands when N. viridula were exposed to tomato with or without glandular trichomes. This led to questioning whether the internal symbionts influence the adaptability of the pest to the host by manipulating saliva compounds. Then, a study can be led to assess the impact of symbiont profiles on pea aphid saliva proteins. We also began to analyse the impact of several diets on stink bugs salivary glands proteome. We use especially proteomic techniques, such as SDS-PAGE and 2D-DIGE followed by LC-MS/MS and MALDI-TOF analysis, but also liquid chromatography gel free approaches. The perspective would be applying saliva or salivary glands extracts on plant in order to screen its defensive responses by complementary “omic” approaches and, in term, to identify candidate proteins playing a role in plant defence induction or bypass. [less ▲]

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See detailImproving laboratory diagnostic capacity of epizootic diseases in Belgium
Cargnel, Mickaël ULg; Roelandt, Sophie; Van der Stede, Yves et al

Poster (2015, October 30)

Belgium and other European neighbouring countries faced several emerging and re-emerging diseases as well as zoonotic diseases over the last decade. However, it has been noticed that during these episodes ... [more ▼]

Belgium and other European neighbouring countries faced several emerging and re-emerging diseases as well as zoonotic diseases over the last decade. However, it has been noticed that during these episodes, the laboratory diagnostic capabilities were surpassed, which led to an increase in the time required for the control and the eradication of these diseases. Belgium is a European hub and can be affected by these diseases which can via different paths e.g. trade of live animals or animal products from around the world or via wildlife. It is therefore crucial to react rapidly to these diseases, to establish contingency plans and to develop appropriate diagnostic tests. Moreover, there are only few publications looking at the issues of increased diagnostic capacities for epizootic diseases based on [less ▲]

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See detailIn vitro and in vivo evaluation of an optimized fenofibrate lipid based solid dispersion produced by a PGSS process
Pestieau, Aude ULg; Lebrun, Sonia; Cahay, Bernard et al

Poster (2015, October 28)

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See detailMASS TRANSFER DURING THE DRYING OF BUILDING MATERIALS
Kahlerras, Loubna ULg

Poster (2015, October 27)

The study of the durability of cementitious materials like mortar which are the object of massive use in building and engineering works implies a perfect understanding of their mass transfer properties ... [more ▼]

The study of the durability of cementitious materials like mortar which are the object of massive use in building and engineering works implies a perfect understanding of their mass transfer properties. This work reports the results of experimental study concerning the influence of water-to cement ratios used for the preparation of the material on its convective drying kinetics. Three tips of mortar were realized and studied with three different water to cement (W/C) ratios; W/C =0.4, W/C = 0.5 and Mortar with ratio W/C = 0.6. The first experimental campaign was done in a convective dryer, using cubic samples of mortar of size (15 X 15 X 10) mm3. The sample was continuously weighed during the drying test and its mass was recorded every 2 minutes in order to obtain the drying curves. These tests present the advantage of reproducing the natural conditions met in the problems of atmosphere-material interactions. A second experimental campaign aimed to characterize from both a textural and hydric point of views, the materials, by mean of DVS (dynamic vapour sorption). The mass diffusivity coefficient and the pore size distribution of mortar were obtained from adsorption-desorption cycles. On the other hand the influence of the air drying humidity during the convective drying in the conditions of velocity, temperature and debit of air checked by the convective dryer was studied. From these campaigns, it was observed that the increase of the ratio W/C engenders an increase of the drying kinetics due to the increase of the initial porosity [less ▲]

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See detailEvaluation isocinétique de la fatigabilité musculaire du genou: qu'en est-il de la reproductibilité intra-dynamomètre et de la compatibilité inter-dynamomètre?
Paulus, Julien ULg; Krecke, Roland; Bosquet, Laurent et al

Poster (2015, October 26)

Abstract Certains aspects de l'isocinétisme, bien que considéré comme un gold standard de l'évaluation musculaire [1], restent à explorer: entre autre la reproductibilité intra-dynamomètre des index de ... [more ▼]

Abstract Certains aspects de l'isocinétisme, bien que considéré comme un gold standard de l'évaluation musculaire [1], restent à explorer: entre autre la reproductibilité intra-dynamomètre des index de fatigue (quotient des n dernières répétitions par les x premières par exemple) et des paramètres mesurés lors d'une épreuve de fatigabilité musculaire pour le genou mais également la compatibilité inter-dynamomètre de cette même épreuve de résistance à la fatigue. Après étude via à un test-retest, la reproductibilité des index de fatigue semble très insuffisante pour une utilisation clinique ou scientifique, au contraire des paramètres mesurés. De plus, après exploration via la réalisation du protocole sur trois modèles différents, il apparait que la reproductibilité insuffisante des fléchisseurs du genou ne permettrait pas de comparer les résultats obtenus sur différents dynamomètres. Introduction Les tests isocinétiques contribuent largement à l'évaluation de la performance musculaire et en sont considérés comme un gold standard [1]. Les tests de fatigabilité peuvent s'avérer complémentaires des épreuves courtes de force notamment pour évaluer un athlète sollicitant particulièrement la filière anaérobie lactique ou dans des contextes pathologiques spécifiques [2]. Bien que Bosquet et al. [3] aient démontré la reproductibilité d'un protocole de fatigabilité pour le genou, 30 flexions-extensions maximales à 180°.s-1 avec une amplitude de 100°, à notre connaissance, aucune étude, ayant pour objectif d'être exhaustive dans son analyse, n'a été menée sur la reproductibilité des paramètres mesurés (meilleure répétition, cumuls total et partiels) et index de fatigue (quotient des n dernières répétitions par les x premières, du cumul des répétitions par la meilleure,…) bien qu'ils soient proposés directement par les dynamomètres et/ou utilisés en clinique et dans la littérature lors de l'étude de la fatigabilité du genou [4]. Sont-ils suffisamment reproductibles pour que leur utilisation soit scientifiquement validée? La compatibilité des résultats d'une épreuve de fatigabilité du genou entre différents dynamomètres isocinétiques n'a également, à notre connaissance, pas été évaluée récemment, qui plus est sur plus de deux dynamomètres de marques différentes [5]. Est-il envisageable de comparer les résultats obtenus sur des dynamomètres de marques différentes et de généraliser les normes? Méthodes Pour tenter d'apporter un élément de réponse à la première question, dix-huit hommes sains, modérément actifs, ont réalisé trente extensions-flexions maximales du genou à trois occasions sur un Biodex System 3 Pro, en observant sept à dix jours de repos entre chaque session. Afin de compiler onze paramètres mesurés et de construire quarante-quatre index, le moment de force maximum (MFM) et le travail maximal (Wmax) de chaque répétition ont été enregistrés. La reproductibilité de chaque paramètre et index a été évaluée via leur ICC (2,1), SEM et MD respectifs. Pour évaluer la compatibilité inter-dynamomètre du Biodex System 3 Pro, du Con-Trex MJ PM-2 et du Cybex Humac CSMI, vingt-et-un sujets sains, modérément actifs, ont réalisé trente extensions-flexions maximales du genou à trois occasions, en observant six à dix jours de repos entre chaque session. Le MFM et le Wmax de chaque répétition ont été enregistrés. La compatibilité inter-dynamomètre deux-à-deux a été évaluée à l'aide des ICC (3,1), SEM et MD des paramètres mesurés.   Résultats En ce qui concerne la reproductibilité intra-dynamomètre, les valeurs d'ICC des paramètres mesurés pour les extenseurs du genou sont quasi-systématiquement supérieures à 0.8 tandis que celles pour les fléchisseurs oscillent entre 0.7 et 0.8. Les index de fatigue présentent quant à eux des ICC inférieurs à 0.7 et 0.5 respectivement pour les extenseurs et fléchisseurs. Les valeurs d'ICC ne diffèrent guère entre le MFM et le Wmax (que ce soit pour les extenseurs ou les fléchisseurs ou pour les paramètres mesurés et les index de fatigue). Pour le volet compatibilité inter-dynamomètre, les ICC des paramètres mesurés lors de l'épreuve de fatigabilité musculaire des extenseurs du genou sont très majoritairement supérieurs à 0.8, quelle que soit la paire de dynamomètres considérée. A contrario, les ICC de ces mêmes paramètres mesurés sont presque tous inférieurs à 0.7 pour les fléchisseurs. Les valeurs d'ICC ne diffèrent guère entre le MFM et le Wmax (que ce soit pour les extenseurs ou les fléchisseurs). Logiquement, la compatibilité inter-dynamométrique des index de fatigue n'a pas été étudiée puisque leur reproductibilité intra-dynamomètre a été démontrée comme insuffisante dans la première partie de notre étude. Discussion Considérant qu'un ICC supérieur à 0.8 soit acceptable pour une utilisation clinique [6], nous pouvons conclure que, compte tenu des valeurs obtenues sur notre population, aucun index de fatigue, bien que séduisant compte tenu de leur capacité théorique à caractériser la décroissance de la performance lors d'une épreuve de fatigabilité, ne semble présenter une reproductibilité intra-dynamométrique suffisante pour une utilisation clinique ou de recherche. Seuls les paramètres mesurés tels que la meilleure répétition, les cumuls total ou partiels apparaissent utilisables, en clinique et dans un contexte de recherche, en raison d'une reproductibilité intra-dynamométrique qui peut être qualifiée de (très) haute voire excellente [7, 8]. Des index de fatigue sont donc fréquemment utilisés alors que leur reproductibilité semble très largement insuffisante. La reproductibilité inter-dynamomètre des paramètres mesurés du protocole de fatigue défini par Bosquet et al. [3] permet leur utilisation clinique pour les extenseurs du genou mais est très insuffisante pour les fléchisseurs. Il apparaît donc nécessaire de réaliser les tests à chaque reprise sur le même dynamomètre dans le cadre de suivis longitudinaux ou des comparaisons transversales impliquant les fléchisseurs du genou. Ce manque de compatibilité inter-dynamomètre implique également que les normes soient spécifiques à chaque modèle de dynamomètre isocinétique. Conclusion Nos résultats indiquent que les index de fatigue ne peuvent être utilisés que ce soit dans un contexte clinique ou de recherche pour l'évaluation isocinétique du genou. Seuls les paramètres mesurés sont suffisamment reproductibles pour être employés. Le manque de compatibilité inter-dynamomètre pour les fléchisseurs du genou impliquent d'utiliser le même dynamomètre isocinétique lors de suivis longitudinaux ou de comparaisons transversales. [less ▲]

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See detailProduction of alumina foundry moulds by additive manufacturing for low and high melting points alloys prototypes
Bister, Geoffroy; Deschuyteneer, Dorian; Hautcoeur, Dominique et al

Poster (2015, October 26)

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See detailDynamics of Internalization and Recycling of the pro-Metastatic Membrane Type 4-Matrix Metalloproteinase (MT4-MMP) in Breast Cancer cells
Truong, Alice ULg; Yip, Cassandre ULg; PAYE, Alexandra ULg et al

Poster (2015, October 26)

MT4-MMP (MMP17) is a glycosyl-phosphatidyl inositol-anchored membrane-type matrix metalloproteinase expressed at the cell surface of human breast cancer cells. In triple negative breast cancer, MT4-MMP ... [more ▼]

MT4-MMP (MMP17) is a glycosyl-phosphatidyl inositol-anchored membrane-type matrix metalloproteinase expressed at the cell surface of human breast cancer cells. In triple negative breast cancer, MT4-MMP promotes primary tumor growth and lung metastases. Recently, we demonstrated that EGFR activation and signaling are enhanced by MT4-MMP in a non-proteolytic dependent manner. While trafficking and internalization of EGFR was extensively investigated, little is known about MT4-MMP. Here, we investigated the dimerization, internalization and recycling dynamics of MT4-MMP and its mutated inactive form MT4-MMP-E249A. We demonstrate that MT4-MMP forms dimers and oligomers at the cell surface, a process that was not inhibited neither by broad-spectrum MMP inhibitors (GM6001 and BB94) nor TIMP-2. MT4-MMP is internalized in early endosomes from 10 minutes to 60 minutes. Once internalized, some amount of MT4-MMP is auto-degraded, whereas its inert form E249A was found intact. Large part of the internalized enzyme was recycled intact at the cell surface. By exploring its endocytosis, we found that MT4-MMP is internalized by the CLIC/GEEC pathway, a mechanism that differs from other MT-MMP members. Overall, we provided a new mechanistic insight on the regulatory mechanisms of MT4-MMP in human breast cancer cells. We also, highlighted unique features of MT4-MMP among membrane-associated MMPs, which may be useful for the design of novel therapeutic strategies for metastatic breast cancer. [less ▲]

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See detailInternational Classification of Primary Care in a cross-lingual terminology portal (Poster)
Jamoulle, Marc ULg; Kuehlein, Thomas; Pizzanelli, Miguel et al

Poster (2015, October 23)

ICPC, reflecting the burden of family medicine, is available in the HeTOP cross-lingual terminology portal (URL: www.hetop.eu) in 19 languages, mostly European (e.g. Spanish, Portuguese) but also in ... [more ▼]

ICPC, reflecting the burden of family medicine, is available in the HeTOP cross-lingual terminology portal (URL: www.hetop.eu) in 19 languages, mostly European (e.g. Spanish, Portuguese) but also in Japanese or Mandarin. The HeTOP interface has been also translated in 10 languages by Wonca colleagues (including in Turkish, Vietnamese and Romanian). [less ▲]

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See detailPour une approche poétique du détournement de jeux vidéo : deux obstacles théoriques
Barnabé, Fanny ULg

Poster (2015, October 23)

Cette recherche prend pour objet les différentes formes de détournement du jeu vidéo, avec pour particularité de les étudier, non pas d’un point de vue sociologique, mais sous un angle formel. La ... [more ▼]

Cette recherche prend pour objet les différentes formes de détournement du jeu vidéo, avec pour particularité de les étudier, non pas d’un point de vue sociologique, mais sous un angle formel. La réception créative qu’est le détournement est donc abordée à travers les « traces d’usage » observables dans les œuvres produites, et ce, dans le but de mesurer et de caractériser l’écart que ces textes « au second degré » construisent par rapport à une pratique consensuelle du jeu. L’objectif final est d’ébaucher une poétique du détournement vidéoludique. [less ▲]

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See detailCommunity based participatory research about the multiculturality of second generation migrants after 50 years of Moroccan and Turkish immigration . 50-50 : The “ elli – khemsin ” project
Tatli, Hülya; Laurent, Geoffroy; Henry, Anne Pascal et al

Poster (2015, October 23)

Key words : Family Practice / Community-Based Participatory Research / Intergenerational Relations / loyalty conflict Introduction “Would you consider yourself more Turkish or more Belgian”? To make a ... [more ▼]

Key words : Family Practice / Community-Based Participatory Research / Intergenerational Relations / loyalty conflict Introduction “Would you consider yourself more Turkish or more Belgian”? To make a choice between Belgium and Turkey is like making one between my mother and my father claims a Belgian-Turkish teenager. That reveals the deep feeling of the descendants of the Moroccan or Turkish immigration in Belgium we commemorate the 50th anniversary this year. When two objects of desire are in contradiction with each other, that may give rise to a loyalty conflict. And that is exactly what we are interested in: the loyalty feeling between parental culture and the one of the native country. This project aims to take interest in the fruits of the immigration and more particularly in the teenagers born of immigrant parents in Belgium by the realization of a video by whom it concerns first, with the help of professionals. How do these teenagers daily manage this far-out situation? The aim of this project is to illustrate these silent questionings. The realized video will be broadcasted in schools to allow other teenagers to identify with the characters and to open up the debate. Methods The non-profit association ETMS (Private health center Espace Temps) has signed a partnership declaration with the Regional Center of Immigration of Charleroi and the non-profit association Accompaniment of Teenagers in Open Custody (AJMO) to elaborate a participatory research in community health. Recruitment of participants, meetings with the teenagers, storylines writing, shooting, editing and presentation of the video to an interested audience were the following steps. Results A psychologist, a family doctor, a nurse practitioner, a cameraman, a social worker, an educator and six teenagers of Turkish, Moroccan or Algerian origin take part to the project that has lasted for one year. The video will be ready end 2015 and will allow to debate sensitive topics and the creation of a partnership with schools that are interested in using this tool for educational purposes. [less ▲]

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See detailConcept identification and context analysis of the Wonca Europe 2015 book by instance-based categorization of general practice activities
Jamoulle, Marc ULg; Steylaert, Carl

Poster (2015, October 22)

Context: Family physicians are large producers of knowledge. The site of Wonca Europe provides access to almost 20,000 abstracts produced by family physicians since 1995 (from Europe) and 1998 (from World ... [more ▼]

Context: Family physicians are large producers of knowledge. The site of Wonca Europe provides access to almost 20,000 abstracts produced by family physicians since 1995 (from Europe) and 1998 (from World Conferences). On the occasion of 20 years of Wonca Europe, an editorial committee selected 100 of the best abstracts and has asked each author (or a substitute) to make a chapter for the Wonca Book published in 2015 at the Wonca Europe congress. The content analysis of medical texts is generally achieved by the use of MeSH. To fill gaps in MeSH, a specific family medicine classification has been developed under the name Core Content Classification of General Practice / Family Medicine (3CGP). A combination of ICPC + Q-Codes (new tool for non-clinical items) provides just fewer than 900 concepts for analyzing the contents of specific texts to family medicine. Method: The 100 chapters of the Wonca Book 2015 were analyzed using 3CGP by a single observer not involved in the editorial committee of the book. Every text has been carefully read and a maximum of two concepts by chapter were reported in an Excel spreadsheet. Result: 150 codes were used of which 143 from the classification of non-clinical items Q-codes and 7 coming from ICPC. Among the topics discussed by 143 authors and identified by Q-codes, 35 relate to knowledge management which includes family medicine education, 29 codes concern research and development, 29 doctor's specific area, 24 patients’ issues, 11 relate to the structure and 8 deal with ethics. Discussion: Despite limitations (unique observer – not validated tools) the study reveals striking facts. It is noted that the editorial board has favored themes that affect the structure and organization of knowledge of the profession. Clinical items or affecting directly the patients are very poorly represented. More about 3CGP on http://docpatient.net/3CGP The chapter of the book are also used as source of knowledge in the Q-Codes content study (ongoing) available on www.hetop.eu (inscription needed) [less ▲]

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See detailSetting up an eddy covariance system to measure N2O fluxes exchanged by a production crop - First steps
Lognoul, Margaux ULg; SALERNO, Giovanni ULg; Debacq, Alain ULg et al

Poster (2015, October 20)

In order to study N2O exchanges by a Belgian production crop, we installed an eddy covariance system at the Terrestrial Observatory of Lonzée (Belgium), using a H2O and N2O quantum cascade laser analyzer ... [more ▼]

In order to study N2O exchanges by a Belgian production crop, we installed an eddy covariance system at the Terrestrial Observatory of Lonzée (Belgium), using a H2O and N2O quantum cascade laser analyzer and a sonic anemometer. We obtained three days of measurements and were able to investigate data preprocessing and flux calculation. We observed a drifting time-lag between the analyzer and the anemometers time series, presumably caused by an internal clock drift. Time-lag determination (using the covariance function maximum method) was more difficult for N2O than H2O, suggesting that this routine should be adapted to gas characterized by low fluxes. We investigated high frequency loss and found a system cut-off frequency of 0.5Hz for H2O, comparing its cospectrum to sensible heat cospectrum. We were not able to retrieve a neat cospectrum for N2O because of low fluxes during turbulent conditions. Further work and more data will be needed in order to bring answers to pending questions. [less ▲]

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See detailOral administration of a spirulina extract protects old mice against hepatic "inflammaging"
Neyrinck, A.; Taminiau, Bernard ULg; Daube, Georges ULg et al

Poster (2015, October 20)

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See detailQuantification of somatic calcium currents in midbrain dopaminergic neurons
Philippart, Fabian ULg

Poster (2015, October 19)

Dopaminergic neurons (DA) within the substantia nigra pars compacta (SNc) and ventral tegmantal area (VTA) are autonomous pacemakers. SNc DA neurons are particularly prone to degeneration in Parkinson’s ... [more ▼]

Dopaminergic neurons (DA) within the substantia nigra pars compacta (SNc) and ventral tegmantal area (VTA) are autonomous pacemakers. SNc DA neurons are particularly prone to degeneration in Parkinson’s disease (PD). However, the cause for their high vulnerability to degeneration, compared to the neighboring, more resistant VTA DA neurons, remains unclear. It has been suggested that the pacemaking in SNc DA neurons relies on L-type calcium channels. A large calcium entry through these channels could cause oxidative stress in SNc DA neurons and could explain their vulnerability. We directly tested this hypothesis in young rats by quantifying the density of L-type calcium channels in the soma of DA neurons using nucleated patch-clamp recordings. Our results show measurable L-type (sensitive to 5µM nifedipine) and non L-type calcium currents in the soma of SNc and VTA DA neurons. We found that the amplitude of the nifedipine-sensitive current is higher in SNc DA neurons (17.8±1.6 pA/pF; n=10) than in VTA DA neurons (11.2±1.8 pA/pF; n=10). Interestingly, we noticed that the L-type current is the predominant Ca2+ current in SNc DA neurons (44%) while this is not the case in VTA DA neurons (22%). These results were confirmed by non-stationary-fluctuation analysis (NSFA). These experiments revealed a higher mean number of nifedipine-sensitive Ca2+ channels in the soma of the SNc DA neurons (502±74; n=6) than in VTA DA neurons (160±45; n=6). The greater density of L-type channels in the soma of SNc DA neurons could contribute to their vulnerability in human PD patients. [less ▲]

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See detailÉtude des Expériences Corporelles Anormales dans la schizophrénie
Lancellotti, Elisa; Englebert, Jérôme ULg

Poster (2015, October 16)

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See detailEffect of a CpG-ODN on the innate immune system of the horse: an in-vivo trial
Tosi, Irène ULg; Pirottin, Dimitri ULg; Fievez, Laurence ULg et al

Poster (2015, October 16)

Oligodeoxynucleotides containing cytosine-phosphate-guanosine motifs (CpG-ODN) represent a class of agonists of Toll-like Receptor 9 (TLR9). TLR9 activation induces the secretion of cytokines and the ... [more ▼]

Oligodeoxynucleotides containing cytosine-phosphate-guanosine motifs (CpG-ODN) represent a class of agonists of Toll-like Receptor 9 (TLR9). TLR9 activation induces the secretion of cytokines and the maturation of immune cells, thus initiating both innate and adaptive immune responses. Therefore, CpG-ODN has been investigated in different species as a potential immune-modulator targeting infectious, allergic and neoplastic diseases. It has been administered by nebulisation to RAO-affected horses with promising results. Nonetheless, there is no in-vivo study on the effect of CpG administered systemically to the horse. Therefore, we tested the effect of CpG, given by intramuscular injection, on the equine immune response. Eight horses were used for this study. Five mg/horse were injected to 4 horses at D0 and D7; the other horses received a placebo (PBS). Blood was collected 2 days prior to each injection, then regularly up to D21. A clinical exam was realised daily. Laboratory analyses included haematology, ELISA tests for IFN-alpha, IFN-gamma, TNF-alpha and IL-10 and cytometry analyses for MCHII and CD86 expressions on B-lymphocytes. A cross-over of the 2 groups was realised after 2 months of washout. CpG was well tolerated. Significant transient eosinopenia, monocytosis and leukopenia were observed after CpG injection, while ELISA and cytometry analyses did not reveal any significant modification. This trial represents the first in-vivo study where CpG is administered systemically to healthy horses. Further studies are needed to adjust the dose, the formulation and the sampling schedule and to fully investigate this molecule as potentiel modulator of the equine immune system. [less ▲]

Detailed reference viewed: 17 (3 ULg)
See detailPhylogenomic comparison of 16 O5 Shigatoxigenic Escherichia coli (STEC) isolated from young calves and humans with diarrhoea
Fakih, I.; Thiry, D.; Ogura, Y. et al

Poster (2015, October 16)

Detailed reference viewed: 4 (1 ULg)
See detailIdentification and traceability of animal and human faecal contamination in bathing sites in Wallonia
Taminiau, Bernard ULg; Hanon, M.; Nezer, Carine et al

Poster (2015, October 16)

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See detailLong term culture, cryopreservation and genetic modification of chicken primordial germ cells
Tonus, Céline ULg; Garcia Gil, Francisco José ULg; Cloquette, Karine et al

Poster (2015, October 16)

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties ... [more ▼]

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties in vitro and are foreseen as promising tools for developing efficient avian genetic engineering and preservation of germplasm. We propose original methods that allow long term expansion, efficient cryopreservation and genetic modification of primary cultures of undifferentiated PGCs. PGCs are collected from embryonic blood during their migratory period and grown in cell-culture insert in the presence of feeder cells (BRL). This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of PGCs lines. Forty percent of blood samples gave rise to lines originating from three commercial layer and two Belgian endangered breeds. PGCs lines were characterized for the expression of the stem cells and PGCs marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. All lines were male although isolated from pooled male and female blood samples. Two cryopreservation methods were developed based upon slow-freezing and aseptic vitrification. Both have shown a similar effectiveness in allowing storage without phenotype drift. Stably expressing lines were obtained by Lipofectamine® mediated transfection of a GFP plasmid. PGCs were subsequently injected in recipient embryos. Persistence of exogenous PGCs in the developing gonad of recipient embryos confirmed that PGCs retain their gonadal colonisation ability, both after long-term culture and after cryopreservation. [less ▲]

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See detailEvaluation de la connaissance relationnelle chez les enfants dysphasiques
Krzemien, Magali ULg; Leroy, Sandrine ULg; Maillart, Christelle ULg

Poster (2015, October 16)

La dysphasie est un trouble spécifique du développement du langage oral qui fait l’objet de nombreuses recherches mais dont certains aspects sont encore mal connus. Les théories explicatives de ce trouble ... [more ▼]

La dysphasie est un trouble spécifique du développement du langage oral qui fait l’objet de nombreuses recherches mais dont certains aspects sont encore mal connus. Les théories explicatives de ce trouble prolifèrent, notamment suite au développement de l’approche cognitivo-fonctionnelle du langage qui permet de concevoir celui-ci comme étant en interaction avec des processus généraux et non-linguistiques. En particulier, la théorie des grammaires de construction soutient que le langage s’acquiert par une abstraction progressive des formes entendues qui deviennent alors des schémas de construction. Ce mécanisme serait lié au raisonnement analogique. Or, les enfants présentant une dysphasie semblent détenir de faibles capacités de raisonnement analogique par rapport à leurs pairs de même âge. Nous nous demandons donc à quoi pourrait être dû ce déficit, en nous intéressant à un élément déterminant du raisonnement analogique : la connaissance relationnelle. Une tâche de scènes analogiques a été proposée à un groupe d’enfants présentant une dysphasie et à deux groupes contrôles, l’un apparié en âge linguistique et l’autre en mémoire de travail. Les participants devaient sélectionner l’élément détenant le même rôle relationnel qu’un élément pointé dans un dessin présenté au préalable. Les relations étaient similaires pour chaque item mais la nature des indices perceptuels variaient selon la condition : dans une condition, les indices perceptuels et relationnels concordaient, alors que dans une autre ils discordaient. Dans une troisième condition, aucune similarité perceptuelle n’était présente. Tous les enfants semblent bénéficier de la congruence entre informations perceptuelles et relationnelles. De plus, les enfants présentant une dysphasie détiennent des compétences de raisonnement analogique semblables à leurs pairs de même âge linguistique et de mêmes capacités de mémoire de travail. Ils semblent également posséder des connaissances relationnelles relativement préservées par rapport à leurs pairs de même âge mais des difficultés apparaissent quand la tâche se complexifie, notamment quand des distracteurs perceptuels doivent être inhibés. Ceci semble pouvoir être expliqué par les troubles langagiers et les déficits en mémoire de travail liés à la dysphasie. De futures études devront néanmoins écarter l’hypothèse d’une différence qualitative des représentations relationnelles entre des enfants présentant une dysphasie et d’autres ayant un développement langagier normal. [less ▲]

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See detailProgress in the etiological diagnosis of bovine abortions: the contribution of 16S rRNA metagenetic analysis
Delooz, L.; Evrard, J.; Grégoire, F. et al

Poster (2015, October 16)

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See detailStudy of the microbial diversity of microbial ring trials by metagenomic analysis : Quantification of alive bacteria by exclusion of dead bacteria
Fall, Papa Abdoulaye; Burteau, Sophie; Detry, Émilie et al

Poster (2015, October 16)

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See detailGoat breeding in the rural district of Chemini (Algeria)
Moula, Nassim ULg; Farnir, Frédéric ULg; Leroy, Pascal ULg et al

Poster (2015, October 16)

The Kabyle goat breed represents 10.52% of the total Algerian market (3.8 million heads). A survey of 69 farmers has been carried out in order to characterize it and its breeding in the District of ... [more ▼]

The Kabyle goat breed represents 10.52% of the total Algerian market (3.8 million heads). A survey of 69 farmers has been carried out in order to characterize it and its breeding in the District of Chemini. The questions focused on household agricultural activities, including breeding of goats, cattle, sheep, rabbits, chicken, turkeys, honeybees and the production of olive oil and figs. The goat morpho-biometric characterization was based on 18 corporal measurements. The Hierarchical Cluster Analysis (HCA) of the farm structures defined four groups of farms, variance between groups accounting for 55.7% of total variability. The average number of goats in groups 1 to 4 was 7.2±2.8, 11.1±3.5, 22.3±1.4 and 3.4±1.0, respectively. Group 3 (n=6), showed the greatest number; it consists of older farmers (67 years old or over) not practicing arboriculture. They also have the largest numbers of sheep (48.67), rabbits (50.83), chicken (48.33) and turkeys (42). Group 4 (n=9), with the lowest number of goats, was the group of young farmers (39 years old or less), more dedicated to cattle breeding (~52 heads) and the production of olive oil (~207 trees) and figs (~47 trees). The 18 morpho-biometric variables were significantly higher in males than in females (p<0.05). The Kabyle goat is small (male: 68.23±0.97cm and female: 65.41±0.55cm) with long hair (male: 12.24±0.51cm, and female: 9.51±0.29cm). Its ears are drooping, its convex profile has a slightly pronounced nasal split and its dress color ranges from dark brown to black. This adapted genetic resource should be key in the development of a local production, based on a strong commitment of farmers inside a breed association, for the production of specimens corresponding to a standard, to be determined collectively. [less ▲]

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See detailL’infraction sexuelle comme recherche de bonheur ?
Dziewa, Amandine; Glowacz, Fabienne ULg

Poster (2015, October 16)

Detailed reference viewed: 25 (1 ULg)
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See detailÉtude du corps-pour-autrui dans l'anorexie mentale
Minguet, Eugénie; Englebert, Jérôme ULg

Poster (2015, October 16)

Detailed reference viewed: 54 (7 ULg)
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See detailPt-Ni Hollow Nanoparticles for Oxygen Reduction Reaction: Controlling the Nanoparticles Morpholo-gy
Asset, Tristan ULg; Chattot, Raphaël; Zubiaur, Anthony ULg et al

Poster (2015, October 15)

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See detailBelgian children’s and adolescents’ views on the elderly
Flamion, Allison ULg; Missotten, Pierre ULg; Schroyen, Sarah ULg et al

Poster (2015, October 15)

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See detailBelgian International Lawyers and WWI. Networks, Experience and Memory
Genin, Vincent ULg

Poster (2015, October 15)

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See detailEnvironmental impact assessment of bio-based binders: from production to industrial applications
Gerbinet, Saïcha ULg; Belboom, Sandra ULg; Briard, Vincent et al

Poster (2015, October 15)

A binder is used to hold together the fibers forming the mineral wool products (see figure 1). These fibers can be produced from sand and recycled glass for glass wool products (see figure 2) or from rock ... [more ▼]

A binder is used to hold together the fibers forming the mineral wool products (see figure 1). These fibers can be produced from sand and recycled glass for glass wool products (see figure 2) or from rock (volcanic rock, typically basalt or dolomite) for stone wool products. Traditionally, the binders used in mineral wool products are based on phenol-formaldehyde. Due to sanitary and environmental considerations and increased focus on indoor air quality, the producers developed new alternative binders. Especially, Knauf Insulation, a worldwide building insulation manufacturer, developed a binder based on plant starch and called ECOSE. In addition of not using added phenol-formaldehyde , this new binder is also supposed to reduce the environmental impacts of Knauf Insulation mineral wools. Moreover, due to its properties, others applications are now considered for ECOSE such as composite wood panels [1]. The aim of this study is to determine the environmental impact of ECOSE and to compare it with more traditional binders using Life Cycle Assessment (LCA) methodology. LCA analyzes the environmental aspects and potential impacts associated with all the stages of a product's life. In this type of environmental assessment the energy and material flows for the entire life-cycle are surveyed and analyzed with special attention to possible environmental hazards or human health problems. The ISO 14040 and 14044 norms [2, 3] provide the general guidance for performing an LCA. The LCA methodology is first applied to ECOSE main component: glucose. The presentation will present results for glucose production from cereals starches After that, first ECOSE application, glass mineral wool products will be presented in details, including production process (see figure 3). The modelling of the glass mineral wool production process in LCA software GaBi 6 [4] is then described. One of the model specificity is that it allows to perform LCA of any glass wool products produced in Knauf Insulation plants in Europe. The adaptations to the model to allow studying former glass wool product when using phenol-formaldehyde binders will also be presented such as the advantages of this model. Moreover, preliminary results about ECOSE and phenol-formaldehyde glass wool products are explained. Références [1] Knauf Insulation. [cited 2014; Available from: http://www.knaufinsulation.ua/en. [2] ISO 14044, Environmental management - Life cycle assessment - Requirements and guidelines. 2006. [3] ISO 14040, Environmental management - Life cycle assessment - Principles and framework. 2006. [4] LBP, University of Stuttgart, and PE INTERNATIONAL AG, GaBI 6. 2012: Leinfelden-Echterdingen. p. GaBi 6: Software and database for life cycle engineering. [less ▲]

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See detailAgeism among nurses in oncology
Schroyen, Sarah ULg; Missotten, Pierre ULg; JERUSALEM, Guy ULg et al

Poster (2015, October 14)

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See detailHPLC-DAD IDENTIFICATION OF SOME FLAVONOIDS FROM THE LEAVES AND AERIAL PARTS OF BRYONIA ALBA L. SPECIES SPONTANEOUS IN THE ROMANIAN FLORA
Ielciu, Irina-Ioana ULg; Păltinean, Ramona; Cieckiewicz, Ewa ULg et al

Poster (2015, October 14)

Bryonia alba L. is a climbing species, spontaneous in the Romanian flora, which can be found throughout the whole country [1]. It is known for its cytotoxic, analgesic, antipyretic, anti-inflammatory ... [more ▼]

Bryonia alba L. is a climbing species, spontaneous in the Romanian flora, which can be found throughout the whole country [1]. It is known for its cytotoxic, analgesic, antipyretic, anti-inflammatory, antibacterial, anti-rheumatic, laxative-purgative and smooth muscle relaxant proprieties, being used both in traditional medicine and in homeopathy [2]. The main objective of this study consists in the evaluation of the flavonoid profile of this species. The vegetal material was harvested from the spontaneous flora of Cluj county (Romania). The vegetal extracts were obtained by ultrasonication, in methanol. Analysis of flavonoids was performed by a HPLC-DAD method and revealed mainly the presence of C-glycosides, of which saponarine was found as the main compound. Quantification of saponarin was also performed, using the HPLC method, on samples collected at different periods of time. Variation of the quantity of saponarine according to harvested samples was determined. Further analysis are under process in order to investigate the structure of these flavonoids and the pharmacological effects of the Bryonia alba L. plant extracts. References: 1. *** Flora Europea, vol. 2, Cambridge, Univ. Press. Cambridge London-New York Melbourne, 1979, p. 298-299 2. Demarque D, Jouanny J, Poitevin B, Saint Jean Y. Pharmacologie et matière médicale homéopathique, 3ième edition, France, CEDH, 2007. [less ▲]

Detailed reference viewed: 88 (6 ULg)
See detailNew horizons in the etiological diagnosis of bovine abortions, the contribution of 16S rDNA sequencing
Delooz, L.; Houtain, J.-Y.; Grégoire, F. et al

Poster (2015, October 14)

Detailed reference viewed: 6 (1 ULg)
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See detailControl of CXCL8/IL-8 expression by ZO-1:
Lesage, J; Suarez-Carmona, Meggy ULg; Grelet, S et al

Poster (2015, October 12)

Detailed reference viewed: 21 (1 ULg)
See detailPROTEKER : Mise en place d’un observatoire sous-marin côtier aux Îles Kerguelen
Saucède, T; Améziane, N; Feral, JP et al

Poster (2015, October 10)

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See detailVISA-PF: A Cross-Cultural Adaptation And Validation Of The VISA-P Questionnaire In French
Kaux, Jean-François ULg; Delvaux, François ULg; Oppong-Kyei, Julian et al

Poster (2015, October 08)

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See detailApplying Stable Isotopes Bayesian Ellipses (SIBER) to characterise trophic niches of large cetaceans from the north-Western Medterranean Sea
Pinzone, Marianna ULg; Michel, Loïc ULg; Ody, Denis et al

Poster (2015, October 08)

Diet composition and feeding preferences are of critical importance to understand a species' ecology; better knowledge of these matters is necessary for efficient conservation. Few is known on the trophic ... [more ▼]

Diet composition and feeding preferences are of critical importance to understand a species' ecology; better knowledge of these matters is necessary for efficient conservation. Few is known on the trophic ecology of Mediterranean cetacean populations and the elusiveness of these organisms limits acquisition of new information. Here, we analysed C and N isotopic ratios of skin biopsies of 17 fin whales Balaenoptera physalus, 15 long-finned pilot whales Globicephala melas and 25 sperm whales Physeter macrocephalus sampled through darting between summer 2010 and 2013 in the North-Western Mediterranean Sea. We subsequently used the SIBER R package to explore isotopic niche parameters as a proxy for trophic niches assessment. Fitting of standard ellipses to each species revealed that no niche overlap between the odontocetes and fin whales was present, in accordance with the lower trophic level of the latter. Moreover, overlap between the isotopic niches of the two odontocetes was limited, confirming resource partitioning between these two species. This could be linked with differences in hunting periods and depths and consequently in prey availability. Bayesian modelling of standard ellipses revealed that the isotopic niche of fin whales was larger than the two odontocetes in over 99.80% of 106 model simulations. It is the first time that such variability is observed in Mediterranean fin whales. This suggests possible exploitation of food items from different trophic levels (krill, small fishes) or from other regions, such as other areas in the Mediterranean Sea and the North-East Atlantic. Modelling also suggested that no meaningful differences were present in the width of isotopic niches of the two odontocetes. The very narrow isotopic niche of pilot whales is in strong contrast with the generalist feeding behaviour this species is believed to have according to literature analysis. Our results open new perspectives on the ecological role of Mediterranean cetaceans. [less ▲]

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See detailCross-Cultural Adaptation And Validation Of The VISA-A Questionnaire In French
Kaux, Jean-François ULg; DELVAUX, François ULg; Oppong-Kyei, Julian et al

Poster (2015, October 08)

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See detailCharacterisation of TLR7/8 in equine pulmonary alveolar macrophages
Tosi, Irène ULg; Frellstedt, Linda; Pirottin, Dimitri ULg et al

Poster (2015, October 08)

Purpose of the study: In both human and equine athletes, viral infections are common causes of respiratory diseases and of a sudden deterioration of expected performances. In both species, the underlying ... [more ▼]

Purpose of the study: In both human and equine athletes, viral infections are common causes of respiratory diseases and of a sudden deterioration of expected performances. In both species, the underlying mechanisms are still unclear, and an involvement of Toll-Like Receptors (TLRs), a fundamental link between innate and adaptive immunity, has been advocated. Our objectives were to verify the presence of TLR7 and TLR8, responsible for the early anti-viral response in mammals, in equine pulmonary alveolar macrophages (PAMs) and to assess their function through specific stimulation. Methods used: Equine PAMs were collected by broncho-alveolar lavage (BAL), isolated by adherence and stimulated with specific TLR7/8 ligands (an imidazoquinoline compound and single-stranded RNA), mimicking a viral attack. The expression of TLR7/8 was evaluated by rt-PCR and the ligand-induced production of cytokines (type I-IFNs and TNF-α) was assessed via ELISA. Summary of results: Our study demonstrated the expression of TLR7/8 in equine PAMs. QPCR analyses showed a high relative expression of genes coding for TLR7 and TLR8 on equine PAM. Stimulation with specific TLR7/8 ligands resulted in significantly up-regulated production of IFN-β and TNF-α, thereby confirming that TLR7/8 are functional in equine PAMs and that they play a role in the early pulmonary antiviral response. Conclusions: This study shows that TLR7 and TLR8 are present and functional in equine PAM and that they could play a role in the early pulmonary antiviral response. In terms of future perspectives, it is interesting to suggest that the extensively demonstrated efficacy of TLR7 and TLR8 synthetic ligands in the treatment of viral diseases in human medicine could motivate the pursuit of clinical trials in the equine patient for the therapeutic management or prevention of viral respiratory infections. [less ▲]

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See detailLandings profile of small-scale fisheries in Senegal: The case of the Kayar Marine Protected Area
Gueye, Ndiapaly; Diop, Mamadou; Denis, Antoine ULg et al

Poster (2015, October 08)

As marine fisheries in Senegal are in serious decline due to overexploitation, the management of sustainable fisheries requires effective tactics based on scientific data. In order to characterize the ... [more ▼]

As marine fisheries in Senegal are in serious decline due to overexploitation, the management of sustainable fisheries requires effective tactics based on scientific data. In order to characterize the current state of the artisanal fishery of the Kayar Marine Protected Area (MPA), a daily survey of fish landings was carried out between October 2013 and May 2015, and a database was created. Results showed that the landings are largely dominated by pelagic species in terms of weight. In total, 84 taxa were registered, including the species Sardinella aurita, which has been the most landed (30.5%), followed by Trichiurus lepturus (18.9%). These species are mainly captured by means of purse seine. The other types of fishing gear used in the area are the long line and the single line, which target particular species (e.g., Epinephelus aeneus). The temporal evolution of landings shows a period of abundance between May and June, while fishing effort is generally increasing. Regarding the spatial distribution, landings originate primarily from the MPA, where the landing per unit of effort was higher in the Angleterre area compared to other studied areas. These temporal and spatial variations seem to be related to the upwelling intensity which is highly seasonal, habitat features (i.e., presence of an underwater canyon in the MPA), the ecological behaviour of fish species and overexploitation. Our results confirm the need for detailed and extensive population monitoring across fish species. As a result of this study, we hope to provide the first essential information for the integrated management of fisheries in the Kayar MPA and its surrounding area. [less ▲]

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See detailDecrease of carbon tetrachloride (CCl4) over 2004-2013 as inferred from global occultation measurements with ACE-FTS
Mahieu, Emmanuel ULg; Bernath, P. F.; Boone, C. D. et al

Poster (2015, October 05)

In this contribution, we use infrared solar occultation measurements performed by the ACE-FTS (Atmospheric Chemistry Experiment – Fourier Transform Spectrometer) instrument onboard the SCISAT-1 Canadian ... [more ▼]

In this contribution, we use infrared solar occultation measurements performed by the ACE-FTS (Atmospheric Chemistry Experiment – Fourier Transform Spectrometer) instrument onboard the SCISAT-1 Canadian satellite (Bernath et al., 2005). Since its launch in August 2003, this spectrometer has been in continuous operation with no significant degradation of its performance, and global measurements are available from late February 2004 onwards, spanning now more than a decade. [less ▲]

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See detailEpistasis associated to inflammatory bowel disease (IBD) in humans
Gusareva, Elena ULg; Wei, Zhi; Traherne, J.A. et al

Poster (2015, October 05)

Gene-gene interactions underlie biochemical pathways and have been well demonstrated in model organisms. Very few examples exist on replicated epistasis in humans. Here, we performed genome-wide scans to ... [more ▼]

Gene-gene interactions underlie biochemical pathways and have been well demonstrated in model organisms. Very few examples exist on replicated epistasis in humans. Here, we performed genome-wide scans to detect epistasis associated to Crohn’s disease (CD) and ulcerative colitis (UC). We used extensive data of the IIBDGC consisting of 18277 and 14224 CD and UC patients, respectively, and ~34050 healthy controls from 15 European countries typed on the Immunochip. At first, we removed rare variants at MAF<0.05 and filtered common variants at linkage disequilibrium (LD) of r2>0.75. To limit our results to independent effects, SNPs on chromosome 6 (which contains the HLA locus), were furthermore pruned to ensure an LD of r2<0.35. We adjusted the binary traits, CD and UC, for population stratification by regressing out the first 5 principal components in R-3.0.1. The study cohorts were randomly stratified into two subgroups (referred as discovery and replication). We then performed screenings for epistatic interactions with new adjusted trait values in the two subgroups using multidimensional reduction tool MB-MDR with permutation-based (step-down MaxT) multiple testing correction and significance assessment at 0.05. We identified 14 and 6 SNP-pairs associated to CD and UC, respectively, which were concordant between the discovery and replication groups. All SNP-pairs involved concomitant variants located on the same chromosomes (for CD at 1p31.3, 5p13.1, 16q12.1 and for UC at 1p31.3, 6p21.3). A more detailed investigation of these findings, as well as the implementation of different analysis protocols, will further increase our understanding of possible epistatic mechanisms underpinning IBD. [less ▲]

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See detailDetecting patient subgroups using reduced set of disease-related markers with iterative pruning Principal Component Analysis (ipPCA)
Chaichoompu, Kridsadakorn ULg; Cleynen, Isabelle; Fouladi, Ramouna ULg et al

Poster (2015, October 03)

Genetic markers such as Single Nucleotide Polymorphisms (SNPs) can be used to find subgroups of populations or patients with carefully selected clustering algorithms. The iterative pruning principal ... [more ▼]

Genetic markers such as Single Nucleotide Polymorphisms (SNPs) can be used to find subgroups of populations or patients with carefully selected clustering algorithms. The iterative pruning principal component analysis (ipPCA) has been shown to be a powerful tool to identify fine substructures within general populations based on SNP profiles. Usually, SNPs contributing to such profiles have passed rigorous quality control procedures, similar to the ones used for GWAs. Alternatively, attention is restricted to a smaller subset such as PCA-correlated SNPs. Here, we applied ipPCA on real-life data consisting of the 163 known inflammatory-bowel disease (IBD) associated loci in 13,400 healthy individuals and 29,500 IBD (16,902 Crohn’s disease (CD), and 12,598 ulcerative colitis (UC)) patients from the IIBDGC. Prior to clustering by ipPCA, in each group separately, we regressed out the first five Principal Components (PCs) that were computed from a filtered panel of genome-wide SNPs, to account for general population strata. Next, we applied ipPCA on the healthy group, to learn about the presence of a population-specific partitioning in controls. Then we performed three subphenotype analyses: CD only, UC only and the combined group of CD and UC patients (IBD). For each patient subgroup analysis and for the ipPCA analysis on controls, we highlighted and compared the key SNP drivers. CD patients could be molecularly reclassified in two groups, and similar for UC patients. The combined patient group could be subdivided in four groups. Finally, we compared demographic and clinical features among the different groups and looked for meaningful characterizations of adjusted patient clusters by performing pathway analysis on driver genes. [less ▲]

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See detailLa prise en charge interdisciplinaire des patients lithiasiques réduit-elle à long terme le taux de geste urologique pour récidive ?
Castiglione, Vincent ULg; Pieroni, Laurence; Conort, Pierre et al

Poster (2015, October 01)

Certains patients lithiasiques sont hautement récidivants, malgré les progrès considérables en urologie et une prise en charge des facteurs de risque. Nous avons fait l’hypothèse qu’une prise en charge ... [more ▼]

Certains patients lithiasiques sont hautement récidivants, malgré les progrès considérables en urologie et une prise en charge des facteurs de risque. Nous avons fait l’hypothèse qu’une prise en charge interdisciplinaire au long court dédiée au diagnostic du processus lithogène, à l’analyse des causes de récidive, et à la détermination d'objectifs chiffrés de prévention, réduirait le nombre de gestes chirurgicaux pour récidive lithiasique et améliorerait ainsi la qualité de vie des patients. [less ▲]

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See detailFungal biofilm reactor improves the quality of a fusion protein GLA::GFP produced by Aspergillus oryzae
Zune, Quentin ULg; Delepierre, Anissa ULg; Bauwens, Julien ULg et al

Poster (2015, October)

Fungal biofilm is known to promote the excretion of secondary metabolites, in accordance with solid-state related physiological mechanisms. In this work, the potentialities of fungal biofilm will be ... [more ▼]

Fungal biofilm is known to promote the excretion of secondary metabolites, in accordance with solid-state related physiological mechanisms. In this work, the potentialities of fungal biofilm will be investigated in the context of the production of a Gla::GFP fusion protein by Aspergillus oryzae. Since the production of this protein is under the control of the promoter glaB, specifically induced in solid-state fermentation, biofilm mode of culture is expected to enhance the global productivity. However, we found that the glaB promoter is also activated in submerged bioreactor and the fusion protein production is higher in this mode of culture. This result is related to the high shear stress leading to biomass autolysis and leakage of intracellular fusion protein into the extracellular medium. Moreover, 2D-gel electrophoresis highlights preservation of the fusion protein integrity produced in biofilm conditions whereas proteolysis strongly affects fusion protein recovery in the submerged cultures performed at high stirring rate. Fungal biofilm reactor design was then further investigated and the scale-up potentialities were evaluated. Indeed, the specific design investigated in this work involves the use of metal structured packing exhibiting a high specific area and that can be easily expanded to large-scale bioprocessing conditions. [less ▲]

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See detailCanine idopathic pulmonary fibrosis is not associated with herpes virus infection
Roels, Elodie ULg; Dourcy, Mickael ULg; Holopainen, S. et al

Poster (2015, October)

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See detailPALEO-DELTA: Palaeoenvironment and geoarchaeology of the Medjerda delta (Tunisia)
Pleuger, Elisa ULg; Abichou, Hakim; Gadhoum, Ahmed et al

Poster (2015, October)

Phoenician Utica remains today largely unknown, as is its role in the Phoenician expansion in the western Mediterranean. Aristotle and Pliny the Elder mention Utica as a maritime and port city and ... [more ▼]

Phoenician Utica remains today largely unknown, as is its role in the Phoenician expansion in the western Mediterranean. Aristotle and Pliny the Elder mention Utica as a maritime and port city and estimate its origin around the 11th c. BC. However, in the present state of research, no archaeological evidence is earlier than the 9th c. BC, and the location of the Phoenician and Roman port infrastructures remains unknown. Today, the ancient city is located on a promontory in the heart of the Medjerda delta, 10 km inland. This project proposes an interdisciplinary effort to understand the Medjerda delta landscape changes during the Holocene. It starts from an archaeological problem and proposes the contribution of geoarchaeology to the understanding of the relationship between ancient societies and their environment. The fluvial palaeoenvironments and sedimentary processes are studied through the mechanical extraction of cores (15-20 m deep) to reach the early Holocene. Selected sediment samples are then studied in laboratory, using different and complementary approaches. The location of port infrastructures will bring initial answers to the question of the foundation of the city. The study of river palaeoenvironments of the Medjerda delta during the Holocene aim at a better understanding of the nature of the settlement, as well as the function of the city of Utica over time. This study will also assess the impact of the ancient city on the environment and understand how the city adapted to the mobility of this Mediterranean delta. Furthermore, the analysis of sedimentary processes causing the filling of the harbour basin will lead to speculation about the causes of the abandonment of the structures and more generally the decline of the city in favor of Carthage. It will also examine whether natural or anthropogenic factors have influenced this deltaic progradation over the centuries. [less ▲]

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See detailThe Good, the Bad, the Marginal: respiratory management of <29 weeks infants according to subjective assessment of perinatal adaptation.
RIGO, Vincent ULg; KALENGA, Masendu ULg

Poster (2015, October)

Background Even if a primary CPAP strategy gives benefits in extremely preterm infants, many still require intubation at birth. Half of those initially managed with primary CPAP will require further ... [more ▼]

Background Even if a primary CPAP strategy gives benefits in extremely preterm infants, many still require intubation at birth. Half of those initially managed with primary CPAP will require further support: surfactant administration or mechanical ventilation. Those infants have increased risks of death and neonatal morbidities, and will require longer duration of respiratory support. Identifying them early, during the birth stabilization process, might lead to improvements in respiratory care. A subjective classification of perinatal adaptation as Good, Bad or Marginal has been suggested but requires further evaluation. We aimed to evaluate respiratory management according to perinatal adaptation. Methods Premature infants of less than 29 weeks and admitted between 01/2013 and 07/2014 were retrospectively studied. Neonatal database and discharge summaries provided neonatal care and outcome data. Good perinatal adaptation (GPA) was considered for infants with good respiratory drive, tone and low oxygen requirement in the delivery room. Infants with marginal (M) PA had intermittent respiratory drive, normocardia with ventilation, and decreasing FiO2. Bad (B) PA is considered with hypotonia, bradycardia, apnea and high FiO2. Data are presented as mean +/- SD, median (interquartile range) or incidence and analyzed with ANOVA, Kuskal-Wallis test or Chi2. Results Sixteen infants had GPA, 19 MPA and 23 BPA. GA was 26 4/7 wk (24-28) and BW was 885  187g. Risk factors for bad adaptation are (NS) male gender, lower GA, and no complete antenatal steroid exposure. Apgar at 1 min. increases with better PA [B3 (2-5); M6 (3-7) and G8 (7-8)*] (*p<.05 vs B & M), and improves at 5 min.: [B7 (6-7); M7 (6-8); G 9 (8-9)*]. Risk of intubation at birth is associated with poorer adaptation (B 87%; M 47%; G 12%, p<.01) Primary CPAP success was not different according to group (B 3/3; M66%; G56%). Surfactant while on CPAP (LISA method) was given to 11/16 patients, including 7 delivery room administrations. If intubated by day 3, duration of first invasive ventilation was shorter (NS) for GPA (9h) [MPA (15h), BPA (29h)]. Early neonatal death tended to decrease with better PA: 26%, 16% and 0% (p=.08). There is no difference in BPD -36 wk (B 19%, M13%, G 12%). Conclusions Infants with better perinatal adaptation have increased chances of being initially managed with CPAP. Primary CPAP success may be improved with less invasive surfactant therapy. Outside of the delivery room, perinatal adaptation assessment tends to identify risk of early neonatal death, but is not predictive of respiratory outcomes. [less ▲]

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See detailOptmization of culture media for Bifidobacterium bifidum and Bifidobacterium crudilactis and study of the antimicrobial effect of culture supernatants
Bondue, Pauline ULg; Delcenserie, Véronique ULg; Crevecoeur, Sébastien ULg et al

Poster (2015, October)

Complex oligosaccharides from human milk (HMO) contribute to infant health. Bifidobacteriumbifidum mainly found in breast-fed infant microbiota has all the enzymatic machinery for degradation of HMO. On ... [more ▼]

Complex oligosaccharides from human milk (HMO) contribute to infant health. Bifidobacteriumbifidum mainly found in breast-fed infant microbiota has all the enzymatic machinery for degradation of HMO. On the other hand, whey is rich in complex bovin milk oligosaccharides (BMO) very similar to HMO, including 3’-sialyllactose (3’SL). They are very likely to be metabolised by B. bifidum too, but also by B. crudilactis, a bovine origin strain. Fermentation of HMO or BMO by bifidobacteria can result in production of metabolites modulating virulence expression of several pathogenic bacteria. Two strains of bifidobacteria were used in this study: B. bifidum, isolated from breastfed infant feces and B. crudilactis, isolated from bovine raw milk. The ability of those strains to metabolise culture media enriched in glucose, whey and 3’SL has been assessed. Then, the obtained culture supernatant has been tested against virulence genes expression of E. coli O157:H7.  Both strains were able to grow in presence of BMO and 3’SL. B. crudilactis presented the best growth on all media. All culture supernatants obtained after supplementation with 3’SL resulted in significant under-expression of genes ler and qseA. The trend of genes stxB2 and luxS was also toward a down-regulation. BMO combined to some bovine or human origin bifidobacteria strains could be interesting synbiotics to maintain or restore the intestinal health of young children. These effects observed in vitro require further investigations to ensure repeatability in humans and to identify the exact nature of molecules obtained from fermentation media by B. bifidum and B. crudilactis. [less ▲]

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See detailModeling of the cyclic lipodepsipeptide Pseudodesmin A self- assembly through molecular dynamic simulations
Crowet, Jean-Marc ULg; Sinnaeve, Davy; Fehér, Kristina et al

Poster (2015, October)

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See detailMicrobiological risks of the consumption of raw milk and raw milk dairy products
Verraes, C.; Claeys, W.; Cardoen, S. et al

Poster (2015, October)

The Scientific Committee of the Belgian FASFC has published several opinions where the objective was to assess the risks and benefits of the consumption of raw milk and raw dairy products (from multiple ... [more ▼]

The Scientific Committee of the Belgian FASFC has published several opinions where the objective was to assess the risks and benefits of the consumption of raw milk and raw dairy products (from multiple species), based on an elaborate literature study and expert opinion. Raw milk In Belgium, the most relevant microbiological hazards related to the consumption of raw cow, sheep and goat milk are Campylobacter, Salmonella and human pathogenic verocytotoxin producing E. coli (VTEC). Raw donkey and horse milk generally has a high microbial quality. A risk assessment at an European level identified the same hazards and included also Brucella spp. in sheep milk, Mycobacterium bovis in cow milk and tick-borne encephalitis virus in milk from several species. As potential emerging hazards, Coxiella burnetii and Mycobacterium avium subsp. paratuberculosis (MAP) were identified. Raw dairy products In Belgium, the risks of raw dairy products (especially (semi-)soft cheeses) are mainly linked to Listeria monocytogenes, VTEC, Staphylococcus aureus, Salmonella and Campylobacter. Dairy products from cows with subclinical mastitis may contain high numbers of L. monocytogenes and S. aureus. L. monocytogenes, VTEC and S. aureus have been identified as microbiological hazards in raw milk butter and cream albeit to a lesser extent because of a reduced growth potential of these pathogens compared to cheese. In endemic areas in Belgium or abroad, raw dairy products may also be contaminated with Brucella spp., Mycobacterium bovis, the tick-borne encephalitis virus, C. burnetii and MAP. Based on the health threat due to the possible presence of human pathogens, it is stated that heat treatment of milk before consumption and dairy production is important to insure the safety of such products. Concerning so-called beneficial (nutritional and health) effects attributed to raw milk consumption, it was concluded that there is no scientific evidence that, with the exception of an altered organoleptic profile, heating raw milk would substantially change its nutritional value or other hypothesized benefits. The benefits of probiotic and lactic acid bacteria are not relevant due to low numbers encountered in raw milk. [less ▲]

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See detailIrrigation Development Support Program - Agricultural water management in Burkina Faso
Traoré, Farid; Wellens, Joost ULg; Tychon, Bernard ULg et al

Poster (2015, October)

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See detailWhere is the ancient harbour of Utica ? Geoarchaeology and palaeoenvironment of the Medjerda delta (Tunisia)
Pleuger, Elisa ULg; Abichou, Hakim; Gadhoum, Ahmed et al

Poster (2015, October)

Ancient authors mention Utica as a maritime and port city and estimate its origin around the 11th c. BC. However, in the present state of research, no archaeological evidence is earlier than the 9th c. BC ... [more ▼]

Ancient authors mention Utica as a maritime and port city and estimate its origin around the 11th c. BC. However, in the present state of research, no archaeological evidence is earlier than the 9th c. BC, and the location of the Phoenician and Roman port infrastructures remains unknown. Today, the ancient city is located on a promontory in the heart of the Medjerda delta, 10 km inland. This project proposes an interdisciplinary effort to understand the Medjerda delta landscape changes during the Holocene. It starts from an archaeological problem and proposes the contribution of geoarchaeology to the understanding of the relationship between ancient societies and their environment. The fluvial palaeoenvironments and sedimentary processes are studied through the mechanical extraction of cores (15-20 m deep) to reach the early Holocene. Selected sediment samples are then studied in laboratory, using different and complementary approaches. First results permitted to draw an hypothesis of the coastline during Antiquity and to bring out the evidence of an intense sedimentation event post 10th c. BC. The location of port infrastructures will bring initial answers to the question of the foundation of the city. The study of river palaeoenvironments of the Medjerda delta during the Holocene aim at a better understanding of the nature of the settlement, as well as the function of the city of Utica over time. This study will also assess the impact of the ancient city on the environment and understand how the city adapted to the mobility of this Mediterranean delta. Furthermore, the analysis of sedimentary processes causing the filling of the harbour basin will lead to speculation about the causes of the abandonment of the structures and more generally the decline of the city in favor of Carthage. It will also examine whether natural or anthropogenic factors have influenced this deltaic progradation over the centuries. [less ▲]

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See detailUse of a HCV patients' education tool: the knowledge questionnaire with confidence degrees
Kerrad, Amel; Leclercq, Dieudonné ULg; Barreteau, H. et al

Poster (2015, October)

13 patiuent ssuffering of C hepatis have received a pretest on knowledge with confidence degrees accompanying their responses. On the basis of thes responses, they received individual education by a ... [more ▼]

13 patiuent ssuffering of C hepatis have received a pretest on knowledge with confidence degrees accompanying their responses. On the basis of thes responses, they received individual education by a pharmacist about their treatment. A post-test, again with confidence degrees, permitted to measure, according to methods described by Leclercq (2009), to evidence objective and subjective gains in knowledge as well in patients' adherence to treatment. [less ▲]

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See detailUse of NIR hyperspectral imaging and chemometrics to quantify roots and crop residues in soil
Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2015, October)

Discrimination of roots and crop residues, in order to quantify them, based on their Near Infrared spectral signature and chemometrics was tested as a new rapid and reliable method.

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See detailCounseling Program For Caregivers Of People With Alzheimer's Disease: A Case Study
Clesse, Audrey ULg; Adam, Stéphane ULg

Poster (2015, October)

It is widely recognized that living day to day with a family member with Alzheimer's disease is a difficult situation for the relatives. Burden generated by this disease can lead to psychological and ... [more ▼]

It is widely recognized that living day to day with a family member with Alzheimer's disease is a difficult situation for the relatives. Burden generated by this disease can lead to psychological and physical exhaustion for the caregiver, called burnout. Several health studies have shown that caregivers are a population at risk, particularly in terms of depressive symptoms (Covinsky et al., 2003), psychological distress (Pinquart and Sörensen, 2003) or health problems (Schulz et al., 1997). They also present a higher risk of mortality than noncaregivers (Schulz and Beach, 1999). Given these results, we understand the major interest of establishing support program to offer therapeutic monitoring for people caring for a relative with dementia. We present Mrs C.G’s case study, who followed the counselling program developed by Adam et al. (2009). This program aims to meet the specific demands of the caregiver by providing informative elements about the disease, relevant advice based on cognitive functioning through the specific profile of the patient and real daily challenges experienced by the caregiver. The “stress management approach” proposed by Zarit et al. (1987) induces the use of coping strategies focused on problem solving. The comparison between pre and post-intervention brings us positive elements, particularly in enhancement of the caregiver’s quality of life, of his sense of competence and efficiency in managing the disease on a daily basis. About burnout, which was the primary focus this intervention, we find that emotional exhaustion tends to decrease during the counselling sessions. A particularly important finding relates to family changes made throughout the therapy, questioning the organization of family dynamics. These positive results show the importance of developing support programs for caregivers adapted to their needs. Moreover, training therapists for this purpose seems essential because it requires a comprehensive and holistic clinical approach, given the neuropsychological, cognitive behavioural and systemic components involved. [less ▲]

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