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See detailAssessment of the species richness of highly eusocial bees according with habitat in Gabon
Fabre Anguilet, Edgard ULg; Bengone Ndong, Toussaint; Nguyen, Bach Kim ULg et al

Poster (2015, May 18)

Bees are among the most e ective pollinators. These insects include honeybee and stin- gless bee. In Africa, habitat loss is the main threat to these insects. This loss of habitat is largely due to ... [more ▼]

Bees are among the most e ective pollinators. These insects include honeybee and stin- gless bee. In Africa, habitat loss is the main threat to these insects. This loss of habitat is largely due to various human activities. An evaluation of the richness of social bees in three locaties in Gabon (Central Africa) was conducted from October 2013 to March 2014: Kougouleu who underwent deforestation and implantation of food crops, forest area Mas- sengalini in bu er zone of protected area of Lope and Lekokodiba in logging concession of Preccious Woods Gabon. For this, a collection was conducted using yellow traps lled with a mixture of water and honey and using a hand vacuum in 20 sites in each area. The number of species was compared to that estimated there are about thirty years. Eleven species of social bees (Apis mellifera adansonii and ten stingless bee species) were collected, of which 5 to Kougouleu, 8 to Massengalini and 11 to L ekokodiba. Honeybee has been collected in all localities. Forest localities are distinguished from Kougouleu by higher species diversity. This di erence is highly signi cant (Kruskal-Wallis 2 = 45.362, df = 2 and p-value<0.001). A decrease of approximately 50% of the number of stingless bee species in 30 years was found to Kougouleu, while the number of social bee species varies little in forest localities. Our study shows that the loss of forest habitat causes a decline in the species richness of social bees and stingless bees are more sensitive to this loss as the honeybee. [less ▲]

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See detailNew efficient bicomponent organocatalysts for the chemical fixation of CO2 onto epoxides: a theoretical study
Alves, Margot ULg; Méreau, Raphaël; Grignard, Bruno ULg et al

Poster (2015, May 18)

Regarding the economic and environmental issues, valorising CO2 as a C1 feedstock for producing useful building blocks is seducing as it is a free and abundant waste resulting from the human activity ... [more ▼]

Regarding the economic and environmental issues, valorising CO2 as a C1 feedstock for producing useful building blocks is seducing as it is a free and abundant waste resulting from the human activity. Cyclic carbonates are useful compounds that found application as green solvents, electrolytes for lithium battery or monomers for polycarbonates or polyurethanes synthesis. These cyclocarbonates can be synthesized with a total atom economy by chemical fixation of CO2 onto epoxides using organometallic complexes or organocatalysts. To date, although this area of research has been the subject of many studies, the identification and development of (organo)catalysts highly efficient under mild experimental conditions still remains challenging. In this context, we developed a new organocatalytic platform based on the use of ammonium salts in combination with single or double hydrogen bond donor activators derived from fluorinated alcohols that showed unexpected booster effect. In this contribution, the ammonium/fluoroalcohol promoted CO2/propylene oxide coupling was investigated through detailed kinetic studies by IR spectroscopy under pressure and results were compared to the most efficient organocatalysts based on ammonium salts and (multi)phenolic derivatives that were reported in the literature. In order to finely understand the reaction mechanism, this study was completed by molecular modeling. DFT calculations showed that the addition of H-bond donors (HBD) with hexafluoroisopropanol functionalities modified the mechanism of the ammonium promoted coupling of CO2 with epoxides. HBDs dramatically decreased the epoxide ring-opening step barrier which highlights the key role of the fluorinated activators in stabilizing the intermediates and transitions states by inter- and intra-molecular hydrogen bonds. [less ▲]

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See detailAliphatic polyphosphates: a promising family of polymers for drug delivery
Vanslambrouck, Stéphanie ULg; Clément, Benoit; Riva, Raphaël ULg et al

Poster (2015, May 18)

Thanks to their biocompatibility and biodegradability, polyphosphates are appealing polymers for biomedical applications. In contrast to polyesters, polyphosphate properties and functionality are easily ... [more ▼]

Thanks to their biocompatibility and biodegradability, polyphosphates are appealing polymers for biomedical applications. In contrast to polyesters, polyphosphate properties and functionality are easily tuned via the chemical nature of the lateral chains. In this work, a series of amphiphilic PEO-block-polyphosphate copolymers were synthesized by organo-catalyzed ring-opening polymerization of cyclic phosphates. These polymers are directly dissolved in water in the absence of any organic solvent and they self-assemble to form nanoparticles Our work aims at changing the lateral chain of polyphosphates to investigate the influence of this structural modification on (i) the size of the nanoparticles, (ii) the critical aggregation concentration, (iii) the encapsulation of an hydrophobic drug in the core of the nanoparticles and, finally, (iv) the release of the drug. [less ▲]

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See detailVaricella-Zoster Virus (VZV) assemblons interplay with PML bodies
Lebrun, Marielle ULg; Thiry, Marc ULg; Sadzot, Catherine ULg

Poster (2015, May 15)

Using a virus expressing the small capsid protein fused to an eGFP tag (eGFP-ORF23 VZV), we recently identified, in the nuclei of infected cells, the presence of dynamic capsid aggegrates. Because we ... [more ▼]

Using a virus expressing the small capsid protein fused to an eGFP tag (eGFP-ORF23 VZV), we recently identified, in the nuclei of infected cells, the presence of dynamic capsid aggegrates. Because we believe that these structures might represent sites of preferential caspid assembly and by analogy with HSV-1, we referred to them as “VZV assemblons”. Structures resembling these assemblons and identified as capsids entrapped in some “PML-cages” were recently described in the nuclei of wild-type VZV infected cells (Reichelt et al., 2011). We then wonder if there was a link between these independent observations. When we infected MeWo cells in which the expression of each PML subunit is downregulated by shRNA, VZV assemblons still formed. Immunostaining of MeWo cells infected by eGFP-ORF23 VZV with an antibody against the PML protein showed that VZV assemblons only partially colocalize with PML bodies. However, overexpression of PML-I-eGFP in HEK293 cells followed by infection with a tagRFP-T-ORF23 VZV, where the ORF23 protein is fused to a red tag, showed a complete colocalization is complete. The same result was obtained with all tested PML isoforms. This suggests that the partial colocalization in normal cells could be due to the expression level of PML proteins. Altogether, these results suggest that rather than a progressive accumulation of newly formed capsids within PML cages, it is likely that PML protein is recruited to the sites where VZV assemblons develop. It correlates with the fact that the number of PML bodies decreases with the infection. Obviously, even if this phenomenon might impede the egress of a substantial amount of capsids and, in this regard, limit the infection progression, all the tested cell lines are permissive to VZV. It would then be interesting to investigate the relationship between VZV assemblons and PML bodies in latent or non permissive VZV infection models. [less ▲]

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See detailInteraction of HTLV-1 Tax with minichromosome maintenance proteins modulates viral transcription
Barez, Pierre-Yves ULg; Carpentier, Alexandre ULg; Boxus, Mathieu et al

Poster (2015, May 13)

First human retrovirus discovered, HTLV-1 infects approximately twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases that include adult T-cell leukemia (ATL) and a ... [more ▼]

First human retrovirus discovered, HTLV-1 infects approximately twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases that include adult T-cell leukemia (ATL) and a neurodegenerative disorder called HAM/TSP (Human associated myelopathy/ Tropical spastic paraparesis). We are interested in the mechanisms of transformation by the viral Tax oncoprotein. We previously showed that Tax interacts with the minichromosome maintenance MCM2-7 helicase and affects host cell replication (Boxus et al, 2012 Blood 119:151). In this project, we focused on the role of the MCM2-7 complex in transcription. We first show by chromatin immunoprecipitation that the MCM2-7 is recruited onto the 5'-LTR promoter. The 5’-LTR does however not act as a DNA replication origin. In contrast, MCM2-7 activates viral transcription as revealed by luciferase reporter assays. Interaction between Tax and MCM2-7 also affect expression of cellular genes. Together, our data thus demonstrate that the viral promoter is not a replication origin and that interaction between Tax and MCM2-7 is involved in the viral transcription. [less ▲]

Detailed reference viewed: 33 (8 ULg)
Peer Reviewed
See detailGrowth factor-induced angiogenesis requires uPAR on endothelial cells
Paques, Cécile ULg

Poster (2015, May 13)

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See detailMagneto-optical trapping of iron atoms
Crauwels, Justine ULg; Huet, Nicolas ULg; Krins, Stéphanie ULg et al

Poster (2015, May 13)

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See detailInvestigation on a hydrogen maser cavity in the TE111 mode : first results
Van Der Beken, Emeline ULg; Léonard, Daniel; Counet, Arnaud et al

Poster (2015, May 13)

Results of our preliminary investigation on a hydrogen maser cavity in the unusual TE111 mode are presented. Hydrogen masers use the transition at 1420.405MHz between the two hyperfine levels F = 0 and F ... [more ▼]

Results of our preliminary investigation on a hydrogen maser cavity in the unusual TE111 mode are presented. Hydrogen masers use the transition at 1420.405MHz between the two hyperfine levels F = 0 and F = 1 of the 1s1/2 ground state of the hydrogen atom. In contrast to standard hydrogen maser that exploits the TE011 mode, the TE111 mode allows one to design hydrogen masers with significant reduced dimensions, which represents a huge benefit for space applications and in particular for the global positioning system. In view of a future development of a hydrogen maser in the TE111 mode we performed a thorough analysis of the maser physics for this mode. Different simulations carried out are compared with first measurement results obtained for such a cavity. [less ▲]

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See detailChaotic Bohmian trajectories for the hydrogen atom
Cesa, Alexandre ULg; Struyve, Ward ULg; Martin, John ULg

Poster (2015, May 13)

In Bohmian mechanics, a single-particle quantum system is described in part by its wave function and in part by the actual position of the particle. The trajectory of the latter can be computed using the ... [more ▼]

In Bohmian mechanics, a single-particle quantum system is described in part by its wave function and in part by the actual position of the particle. The trajectory of the latter can be computed using the guiding equation. This equation states that the velocity of the particle is proportional to the usual probability current associated with its wave function. In this work, we study the quantum trajectory of a single particle in a Coulomb potential whose eigenstates are the well known eigenstates of the hydrogen atom. More precisely, we focus on the relation between chaotic Bohmian trajectories and the motion of wave function nodes. At wave function nodes i.e., where the wave function vanishes, the velocity is not defined which generically induces vorticity. In order to probe chaos, we compute Poincaré map and we numerically evaluate Lyapounov exponents, which characterize the divergence of close trajectories as time increases. For the 2d Coulomb potential, although the superposition of two eigenstates with different energies can lead to an arbitrary high number of moving nodes of the wave function, the Bohmian trajectories display no trace of chaos. This absence of chaotic behaviour originates from the existence of a constant of motion. Therefore, the motion and the number of nodes do not constitute a sufficient condition for the emergence of chaos in Bohmian mechanics. For superpositions of more than two eigenstates, there is no constant of motion, there are moving nodes and we find that the Bohmian trajectories are chaotic. [less ▲]

Detailed reference viewed: 49 (9 ULg)
See detailAn encapsulation-dehydration method for the long-term storage of cyanobacterial strains in the BCCM/ULC collection.
Crahay, Charlotte ULg; Mari, Maude; Wilmotte, Annick ULg

Poster (2015, May 13)

Long-term genetic and functional stability is a fundamental requirement for the maintenance of microorganisms. Cryopreservation is the preferred method for the long-term storage of many micro-organisms ... [more ▼]

Long-term genetic and functional stability is a fundamental requirement for the maintenance of microorganisms. Cryopreservation is the preferred method for the long-term storage of many micro-organisms, including cyanobacteria. The BCCM/ULC collection currently holds over 200 cyanobacterial strains as living cultures, but only 62 are maintained in a cryo-preserved state. The main limiting factors are the low levels of survival of some strains, as well as the long periods required to recover from cryopreservation, and thus the inability to rapidly deliver strains to clients.. The BRAIN-be project PRESPHOTO (preservation of photosynthetic micro-algae in the BCCM collections) (www.presphoto.ulg.ac.be) aims to improve the preservation of cyanobacterial and diatoms in the BCCM/ULC and BCCM/DCG collections, respec-tively. The encapsulation-dehydration is often considered as a promising alternative to the traditional cryopreservation method for recalcitrant microalgal strains. In this technique, cyanobacterial cultures are entrapped in calcium-alginate beads, osmotically dehydrated (traditionally with sucrose solutions), then evaporatively desiccated in a sterile air flow, or over silica gel and subsequently cryopreserved. In this study, the encapsulation-dehydration method was evaluated as potential long-term preservation technique of cyanobacterial strains. The effects of several factors on the viability of 4 strains have been investigated to determine which are the most important for the successful cryopreservation. In particular, several cryoprotectants (i.e. sucrose, PEG or mannitol), methods of beads dehydration (i.e. under a laminar flow or with silica gel) were tested. We have also compared storage at -70°C and in liquid nitrogen (-196oC). A pre-cultivation step in KCl was also tested for its ability to enhance desiccation tolerance. In addition, the use of alginate dissolution buffer following thawing was also considered. In the final phase of the project, the selected cryopreservation protocols will be tested on a large set of strains. In addition, an independent validation of the protocols will be also performed by both partners of the project (BCCM/DCG and the Culture Collection of Algae and Protozoa). [less ▲]

Detailed reference viewed: 36 (2 ULg)
Peer Reviewed
See detailSurfactin: a receptor-independent bacterial elicitor of plant immunity?
Luzuriaga Loaiza, Walter ULg; Legras, Aurelien; Crowet, Jean-Marc ULg et al

Poster (2015, May 13)

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See detailTransport of Bose-Einstein Condensates through Aharonov-Bohm rings
Chrétien, Renaud ULg; Dujardin, Julien ULg; Petitjean, Cyril ULg et al

Poster (2015, May 13)

We study the one-dimensional (1D) transport properties of an ultracold gas of Bose-Einstein condensed atoms through Aharonov-Bohm (AB) rings. Our system consists of a Bose-Einstein condensate (BEC) that ... [more ▼]

We study the one-dimensional (1D) transport properties of an ultracold gas of Bose-Einstein condensed atoms through Aharonov-Bohm (AB) rings. Our system consists of a Bose-Einstein condensate (BEC) that is outcoupled from a magnetic trap into a 1D waveguide which is made of two semi-infinite leads that join a ring geometry exposed to a magnetic flux φ. We specifically investigate the effects of a small atom-atom contact interaction strength on the AB oscillations. The main numerical tools that we use for this purpose are a mean-field Gross-Pitaevskii (GP) description and the truncated Wigner (tW) method. The latter allows for the description of incoherent transport and corresponds to a classical sampling of the evolution of the quantum bosonic many-body state through effective GP trajectories. We find that resonant transmission peaks move with an increasing interaction strength and can be suppressed for sufficiently strong interaction. We also observe that the coherent transmission blockade due to destructive interference at the AB flux φ = π is very robust with respect to the interaction strength. [less ▲]

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See detailDesign and synthesis of PET-probes targeting AMPA subtype receptors
Deverdenne, François ULg; Claes, Giselle ULg; Goffin, Eric ULg et al

Poster (2015, May 13)

The AMPA subtype of glutamatergic receptors is the main actor in the fast excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the ... [more ▼]

The AMPA subtype of glutamatergic receptors is the main actor in the fast excitatory neurotransmission in the mammalian central nervous system. These receptors are involved in the expression and the maintenance of the long-term potentiation, a phenomenon closely linked to cognitive and memorization processes. Based on experimental data, it also appears that glutamatergic systems are involved in several pathological diseases. For instance, a lack of glutamatergic neurotransmission is observed in cognitive disorders or schizophrenia and an excessive activity is observed in Parkinson or Huntington diseases. The in vivo study of glutamate receptors mapping and its evolution appears to be an essential step for a better understanding of its implications. However, according to the literature, design of such a probe remains difficult due to the lack of specificity of the probes. Taking into account the potential in vitro and in vivo activity and specificity of benzothiadizine dioxides (BTDs) acting as AMPA positive allosteric modulators, we are investing the development of new compounds of this class radiolabeled with a fluorine-18 atom. Hence, we are currently developing new series of BTDs characterized by the presence of a fluorine atom and a 7-phenoxy-substituent that are expected to be more active and more specific. Finally, pharmacological tests to evaluate the best candidates for the radiochemical synthesis and in vivo evaluations are currently in progress. [less ▲]

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See detailCheckpoints modulation by the human T-lymphotropic virus type 1 (HTLV-1) Tax protein : towards new therapeutic approaches
Carpentier, Alexandre ULg; Barez, Pierre-Yves ULg; Boxus, Mathieu et al

Poster (2015, May 13)

HTLV-1 infects approximately 15 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is ... [more ▼]

HTLV-1 infects approximately 15 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is currently no satisfactory treatment for these diseases. Among the proteins encoded by HTLV-1, Tax appears to play an important role in the mechanisms leading to pathogenicity. We are interested in the mechanisms of cell transformation by the Tax viral oncoprotein. In particular, we aim at understanding the interplay between Tax and the DNA damage response (DDR). We show that transient expression of Tax results in DNA damage, cell cycle arrest and activation of the DDR. In fibroblasts, cell cycle arrest occurs at the G1 and G2 phases depending on the p53 background. In contrast, HTLV-1 infected lymphocytes proliferate continuously and appear to be adapted to the checkpoints. This mechanism of checkpoint adaptation thus allows ongoing proliferation despite the presence of genomic lesions. Quantification of the rates of NHEJ and homologous recombination indicates that HTLV-1 infected cells require very efficient DNA repair for survival. Therefore, we propose a novel therapeutic approach based on the principle of synthetic lethality using inhibitors of DNA repair. [less ▲]

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See detailRegulation of Tissue Factor by Epithelial-to-Mesenchymal Transitions: Impacts for the metastatic progression.
Francart, ME; Bourcy, M; Lambert, J et al

Poster (2015, May 13)

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See detailMultiscale model of the human cardiovascular system: healthy and pathological behaviours
Kosta, Sarah ULg; Negroni, Jorge; Lascano, Elena et al

Poster (2015, May 13)

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See detailTowards the identification of cis-elements responsible for the high expression of HMA4, an essential gene for zinc hyperaccumulation in Arabidopsis halleri.
Spielmann, Julien ULg; Nouet, Cécile ULg; Scheepers, Maxime ULg et al

Poster (2015, May 13)

A. halleri is a Zn and Cd hypertolerant and hyperaccumulator plant which stores metals in shoot vacuoles. One key step for hyperaccumulation is a highly efficient root-to-shoot translocation of metals ... [more ▼]

A. halleri is a Zn and Cd hypertolerant and hyperaccumulator plant which stores metals in shoot vacuoles. One key step for hyperaccumulation is a highly efficient root-to-shoot translocation of metals. The Zn/Cd plasmamembrane pump HMA4 (heavy metal ATPase 4) plays a central role in this process and is involved in xylem loading/unloading. Moreover, HMA4 is overexpressed in A. halleri compare to its non-tolerant and non-hyperaccumulator sister, Arabidopsis thaliana. The HMA4 overexpression is determined by a combination of gene triplication and cis-regulatory changes increasing transcription levels of each three HMA4 copies. In this study, we are focusing on the two most active HMA4 promoters and we aim to identify the cis-elements underlying high expression of HMA4 in A. halleri using promoter-reporter constructs. Through deletion analysis, two important regions for a high expression have been identified. Ongoing analysis of more detailed deletions will be presented. [less ▲]

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See detailPRESENCIA, COMPORTAMINETO Y RIESGO AMIBIENTAL DE LOS PRODUCTOS DE CUIDADO PERSONAL (PCPs) EN LA INTERFAZ RÍO-ACUÍFERO
Serra-Roig, M. Pau; Jurado Elices, Anna ULg; Diaz-Cruz, M. Silvia et al

Poster (2015, May 12)

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See detailTiO2 templated films used as photoelectrode for solid-state DSSC applications: Study of the solid electrolyte infiltration by Rutherford Backscattering Spectrometry
Dewalque, Jennifer ULg; Colson, Pierre ULg; Mathis, François et al

Poster (2015, May 10)

Liquid-state dye-sensitized solar cells can suffer from electrolyte evaporation and leakage. Therefore solid-state hole transporting materials are investigated as alternative electrolyte materials ... [more ▼]

Liquid-state dye-sensitized solar cells can suffer from electrolyte evaporation and leakage. Therefore solid-state hole transporting materials are investigated as alternative electrolyte materials. However, in solid-state dye-sensitized solar cells, optimal TiO2 films thickness is limited to a few microns allowing the adsorption of only a low quantity of photoactive dye and thus leading to poor light harvesting and low conversion efficiency. In order to overcome this limitation, high surface area templated films are investigated as alternative to nanocrystalline films prepared by doctor-blade or screen-printing. Moreover, templating is expected to improve the pore accessibility what would promote the solid electrolyte penetration inside the porous network, making possible efficient charge transfers. In this study, films prepared from different structuring agents are discussed in terms of microstructural properties (porosity, crystallinity) as well as effect on the dye loading and Spiro-OMeTAD (2,2',7,7'-tetrakis-(N,N-di-p-methoxyphenylamine)9,9'-spirobifluorene) solid electrolyte filling. Different techniques such as transmission electron microscopy (TEM), atmospheric poroellipsometry (AEP) and UV-visible absorption spectroscopy (UV-vis.) have been used to describe the microstructural features of the films. Besides, we have implemented Rutherford backscattering spectrometry (RBS) as an innovative non-destructive tool to characterize the hole transporting materials infiltration. Templated films show dye loading more than two times higher than nanocrystalline films prepared by doctor-blade or screen-printing and solid electrolyte infiltration up to 88%. [less ▲]

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Peer Reviewed
See detail[18F]FMT: a reliable PET tracer for in vivo evaluation of dopaminergic dysfunction in Parkinson’s Disease rat model.
Seret, Alain ULg; Becker, Guillaume ULg; Bahri, Mohamed Ali ULg et al

Poster (2015, May 09)

Background: Rat models of Parkinson’s disease (PD), such as lesioned rats with 6-hydroxydopamine (6-OHDA), are useful for studying dopamine (DA)-related functions. 6-[18F]fluoro-m-tyrosine (6-[18F]FMT) is ... [more ▼]

Background: Rat models of Parkinson’s disease (PD), such as lesioned rats with 6-hydroxydopamine (6-OHDA), are useful for studying dopamine (DA)-related functions. 6-[18F]fluoro-m-tyrosine (6-[18F]FMT) is an effective PET tracer to evaluate of DA terminals integrity and L-aromatic amino acid decarboxylase (AAAD) metabolic pathway. However, there are currently no available quantitative PET studies using [18F]FMT in 6-OHDA lesioned rats. In this context, we investigated the feasibility of in vivo PET study using [18F]FMT on 6-OHDA PD’s model. Methods: 10 µg of 6-OHDA were injected into the right medial forebrain bundle (MFB) of male Sprague-Dawley rats (n=8). As control, sham-treated rats (n=8) were injected with vehicle only but otherwise treated identically. Striatal DA presynaptic activity was assessed by dynamic [18F]FMT PET, 30 min after benserazide pretreatment. Structural T2-weighted brain images were acquired on a 9.4T MRI and were used for co-registration. After normalization on a MRI template, kinetic analysis was performed by “Patlak Reference” model, using PMOD software. Results: Striatal accumulation of [18F]FMT was observed in rats pretreated with benserazide, a peripheral AAAD inhibitor. As consequence of the 6-OHDA-lesion, significant decrease of [18F]FMT accumulation was recorded in the striatum ipsilateral to the lesion. Lesioned rats had dramatically reduced uptake constant Ki in the ipsilateral striatum compared to the contralateral striatum (p<0.001) and to the ipsilateral striatum of sham-treated rats (p<0.005). The Ki ratio (Ipsi./Contra.) was equivalent to 94% in the sham group and dropped to 41% in the lesioned group. Conclusions: [18F]FMT PET enables us to quantify loss of DA presynaptic function in unilaterally 6-OHDA lesioned rats. These results encourage us to pursue further investigations in a longitudinal way and to monitor the progression of the dopaminergic dysfunction in more moderate and gradual preclinical PD models. [less ▲]

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See detailSpray-drying synthesis of Na3V2(PO4)2F3 as cathode material for Li/Na ion batteries
Eshraghi, Nicolas ULg; Caes, Sébastien ULg; Cloots, Rudi ULg et al

Poster (2015, May 08)

In this communication, Na3V2(PO4)2F3 (NVPF) powder with a NASICON-type structure is synthesized for the first time by spray-drying method. The effects of molar ratio of precursors, inlet temperature and ... [more ▼]

In this communication, Na3V2(PO4)2F3 (NVPF) powder with a NASICON-type structure is synthesized for the first time by spray-drying method. The effects of molar ratio of precursors, inlet temperature and heat treatment temperature on the powder purity and particle morphology were investigated. Structural and microstructural characterizations were carried out by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Best electrochemical properties, from specific capacities of 94 mAh .g-1 at C/15 to 80 mAh.g-1 at 1C in li-ion batteries, were obtained for powders dried at 180°C and fired at 600°C. These preliminary results show that spray-drying technique is suitable for obtaining complex powders with a very good homogeneity leading to very good electrochemical properties. [less ▲]

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See detailCatalytic epuration of biogaz
Claude, Vincent ULg; Toye, Dominique ULg; Heinrichs, Benoît ULg et al

Poster (2015, May 08)

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See detailThe seamod.ro operational stochasting Black Sea forecasting system
Vandenbulcke, Luc ULg; Capet, Arthur; Grégoire, Marilaure ULg et al

Poster (2015, May 08)

Detailed reference viewed: 27 (4 ULg)
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See detailSelf-defining memories and self-concept clarity: A comparative study of depressed patients, bipolar patients and healthy subjects
Wagener, Aurélie ULg; Boulanger, Marie ULg; Blairy, Sylvie ULg

Poster (2015, May 06)

Depressed and bipolar patients suffer from autobiographical memory (AM) deficits. For instance, depressed patients present an overgeneralisation and a mood congruence biases. Indeed, these patients seem ... [more ▼]

Depressed and bipolar patients suffer from autobiographical memory (AM) deficits. For instance, depressed patients present an overgeneralisation and a mood congruence biases. Indeed, these patients seem to have difficulties to recall specific and positive memories. The present study focusses on a particular kind of memories: the self-defining memories (SDMs). SDMs are highly pertinent for self-building: they help to define in the most clearly way our perception of ourselves. Because SDMs have not been studied a lot since yet in these two disorders, our goal is to assess the impact of general AM biases on SDMs. Then, the present study pursues two main aims: 1) To assess SDM’s characteristics, 2) To assess the relationship between SDM and self-concept clarity. To reach our objective, depressed and bipolar patients are compared to healthy subjects. Participants are asked to complete SDMs exercises that are recalling six important events which happened in their life. These SDMs are assessed on four variables: specificity, impact, meaning making, contain. Self-concept clarity and mood are also assessed. Until now, 17 depressed patients, 16 bipolar patients and 18 healthy controls participate in this study. Preliminary results do not highlight difference between our groups on specificity. Marginal effects appear for meaning making with healthy subjects recalling more meaning making events. Results show that depressed patients present a mood congruence. The final results will be presented during the convention, as data-collection is on-going and will end in April. Experimental and clinical implications of our results will be discussed. [less ▲]

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See detailProteome variations in Macrobrachium rosenbergii exposed to chlordecone: a gel-free proteomic approach
Lafontaine, Anne ULg; De Pauw, Edwin ULg; Forget-Leray, Joelle et al

Poster (2015, May 06)

Detailed reference viewed: 56 (7 ULg)
See detailPrioritizing likely causative genes in GWAS identified risk loci for immune-mediated inflammatory disorders using cell-type specific eQTL information.
Docampo Martínez, Elisa ULg; Fang, Ming ULg; Dmitrieva, Joelia Borisnova ULg et al

Poster (2015, May 05)

Background/Purpose: Immune-mediated inflammatory disorders (IMIDs) share many genetic risk factors. Pleiotropy may exist at different levels and most of the underlying mechanisms are still to be uncovered ... [more ▼]

Background/Purpose: Immune-mediated inflammatory disorders (IMIDs) share many genetic risk factors. Pleiotropy may exist at different levels and most of the underlying mechanisms are still to be uncovered. GWAS have identified hundreds of risk loci for IMIDs but causative genes have been identified in only a handful of cases. Recent fine-mapping efforts indicate that only a minority of risk variants are coding. This suggests that most risk variants will be regulatory hence affecting disease risk via eQTL effects. Methods: To aid in the identification of causative genes for IMIDs, we generated transcriptome information (HT12 arrays) for six blood cell types (CD4, CD8, CD19, CD14, CD15 and platelets) and intestinal biopsies at three anatomical locations (ileum, colon, rectum) for 350 healthy Caucasians. The same individuals were genotyped with SNP arrays interrogating > 700K variants, augmented by imputation from the 1KG project. To detect cis-eQTL we tested variants within 0.5 megabase windows centered on the tested probe. The nominal p-value of the best SNP within a cis-window was Sidak-corrected for the window-specific number of independent tests. The corresponding best, Sidak-corrected p-values for each probe were jointly used to estimate their respective false discovery rate.To identify likely causative genes in GWAS identified risk loci variants and also better understand pleiotropic effects, we (i) developed a method that quantifies the correlation between “disease association pattern” (DAP) and “eQTL association pattern” (EAP) and provides an empirical estimate of its significance, and (ii) evaluated the effect of fitting known risk variants as covariates in the eQTL analysis following Nica et al. (2010). We applied both approaches to celiac disease (CE) and rheumatoid arthritis (RA) and the second one to type one diabetes (T1D), multiple sclerosis (MS), systemic lupus erythematosus (SLE), ankylosing spondylitis (AS) and psoriasis (PSO). Results: We detected > 16000 significant cis-eQTL, with a degree of sharing between cell types ranging from 38 to 90% highlighting the utility of our multi-tissue panel. GWAS variants were drivers of ciseQTL effects across the different tissues in 399 tests (23.6%), mostly in CD4 cells, and pinpointing 64 new gene-disease associations (3.7%). The number of shared loci and shared eQTL were highly correlated (rho=0.66).RA and SLE showed the highest degree of sharing. Conclusions: We identified new potential candidate genes for IMIDs and characterized pleiotropic effects through ciseQTL mapping in GWAS loci. These findings could shed a light on IMIDs pathogenesis and co-occurrence. Latest results will be presented. [less ▲]

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See detailHigher male than female recombination rate in cattle is controlled by genetic variants effective in both sexes
Kadri, Naveen Kumar ULg; Harland, Chad ULg; Coppieters, Wouter ULg et al

Poster (2015, May 05)

We herein study genetic recombination in three dairy cattle populations from France, New-Zealand and the Netherlands. We apply a new phasing algorithm extracting familial information suited for large half ... [more ▼]

We herein study genetic recombination in three dairy cattle populations from France, New-Zealand and the Netherlands. We apply a new phasing algorithm extracting familial information suited for large half-sib families to reconstruct haplotypes and detect cross-overs (CO). The software is robust to genotyping and map errors. We identify more than 2,000,000 CO events in sperm cells transmitted by 3008 sires to 94,603 offspring, and more than 500,000 CO events in oocytes transmitted by 11,497 cows to 25,390 offspring. When measured in identical family structures, the average number of CO in males (24.0) was found to be larger than in females (21.8). In males, recombination rates were higher closer to telomeres whereas in females, recombination rates dropped at both centromeres and telomeres (probably as a result of lower informativity). The heritability of the global recombination rate (GRR) was close to 0.20 in males and to 0.08 in females. Genetic correlation ranged from 0.38 to 0.69 depending on the population, indicating that shared variants are influencing GRR in both genders. Haplotype-based genome-wide association studies revealed four genome-wide significant QTL, including two previously identified ones (involving REC8 and RNF212). For all QTLs, there was a positive correlation between haplotype effects across sexes, ranging from 0.35 to 0.68. We selected two reference panels of respectively 122 and 215 bulls sequenced at cover > 15x to impute variants in the New-Zealand and French populations. All variants identified by next-generating sequencing in 5 Mb windows encompassing the QTL peaks were imputed with Beagle in order to perform a sequence-based association study. For three QTLs, we identified missense mutations in genes known to be involved in meiosis among the most significantly associated variants. These variants were perfectly associated with the haplotypes underlying the QTL effects. The variant identified in RNF212 had already been reported, whereas missense mutations in MLH3 (N408S) and HFM1 (S1189L) are new findings. Surprisingly, variants previously identified in REC8 did not capture the QTL effect whereas variants in RNF212B, PPP1R3E, BCL2L2, HOMEZ and PABPN1 had much stronger association with the phenotype. The three missense mutations were significant in both genders with two of them accounting for approximately 10% of the genetic variance in males (the allelic substitution effect being approximately equal to one additional CO per genome). Our results are very different from reports of recombination in other species. For instance, in human, recombination rate is higher in females, distinct variants affect recombination rate in males and females and the genetic correlation is close to 0 whereas in cattle, we observed a higher recombination rate in males controlled by shared variants effective in both sexes. [less ▲]

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See detailUse of GEOS-Chem for the interpretation of long-term FTIR measurements at the Jungfraujoch and other NDACC sites
Mahieu, Emmanuel ULg; Bovy, Benoît ULg; Bader, Whitney ULg et al

Poster (2015, May 04)

We present recent and ongoing investigations using 3D CTM GEOS-Chem model simulations for the interpretation of long-term FTIR measurements performed at selected NDACC sites.

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See detailCarbon dioxide, a cheap bio-sourced building block for cyclic carbonates and non-isocyanate polyurethanes
Gennen, Sandro ULg; Alves, Margot ULg; Tassaing, Thierry et al

Poster (2015, May 03)

Due to concerns about global warming combined with the decrease of fossil resources, new carbon feedstocks that are abundant, renewable, non-toxic, inexpensive and environmentally friendly must be ... [more ▼]

Due to concerns about global warming combined with the decrease of fossil resources, new carbon feedstocks that are abundant, renewable, non-toxic, inexpensive and environmentally friendly must be explored to produce chemicals. Besides the valorization of bio-based raw materials, the chemical transformation of carbon dioxide into added-value products has gained interest in both academia and industry. To date, the chemical fixation of CO2 onto epoxides with the formation of cyclic carbonates is one of the most promising ways to valorize CO2 at an industrial scale. Indeed, cyclic carbonates find applications as electrolytes in lithium ion batteries, as aprotic polar solvents or as useful intermediates for polycarbonates. Cyclic carbonates also react with primary amines to produce 2-hydroxyethylurethane. This reaction can be extrapolated to the synthesis of non-isocyanate polyurethanes (NIPU) by polyaddition of bifunctional cyclic carbonates with diamines.5 This study aims (i) at developing a new highly efficient organocatalyst for the synthesis of cyclic carbonates under mild experimental conditions and (ii) their valorization as monomers to produce non-isocyanate polyurethanes. First, we have identified a bicomponent organocatalyst, composed of a judicious combination of an organocatalyst and an activator, for the very fast synthesis of cyclic carbonates from CO2 and epoxides under very mild reaction conditions. Kinetics of reactions were followed by online Raman spectroscopy measurements under pressure. NMR titrations were realized to evidence the mechanism of activation of this novel organocatalytic system that will be discussed in detail in this talk. The second objective relies on the development of new efficient organocatalysts for the synthesis of high molar masses NIPUs in short reaction times. Organic compounds interacting with the cyclic carbonate by hydrogen bonding were identified and their catalytic activity was demonstrated for model compounds. [less ▲]

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See detailCharacterization of almond kernel oils of five almonds varieties cultivated in Eastern Morocco
Houmy, Nadia ULg; Mansouri, Farid; Ben Moumen, Abdessamad et al

Poster (2015, May 03)

This study focuses on characterization of almond kernel oils extracted mechanically from five sweet almond varieties Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil ... [more ▼]

This study focuses on characterization of almond kernel oils extracted mechanically from five sweet almond varieties Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil content, physicochemical parameters, triacylglycerol and fatty acid compositions were determined. Analyzed oils showed low acidity values that range between 0.77 – 0.88 %, peroxide values range between 6.43 – 16.39 meq/kg and Iodine values range between 98.42 – 103.90%. The principal fatty acid of almond kernel oils is oleic acid (C18:1); oils of Ferragnes-Ferraduel and Beldi varieties show higher values of C18:1 respectively of 72.87 and 71.62 %, however Fournat almond kernel oil shows the lowest content of C18:1 (63.54%). HPLC analysis of triglycerides was carried out, and results show that analyzed almond kernel oils are characterized by the dominance of trioleylglycerol (OOO) that contents range between a minimum of 31.48 % for Fournat’s oil and 43.82% for Ferragnes-Ferraduel’s oil. The oxidative stability of almond kernel oils was determined by rancimat tests as the induction period (IP, h recorded by a 743 Rancimat apparatus Metrohm, Switzerland). Results show that stability, of almond kernel oils is clearly influenced by the almond variety; Oxidative stability of tested almond kernel oils ranged between an IP = 20.28 h for Marcona oil and an IP =27.55 h for Ferragnes-Ferraduel. [less ▲]

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See detailLarge-scale polarization alignments of quasars in the JVAS/CLASS 8.4-GHz surveys
Pelgrims, Vincent ULg; Hutsemekers, Damien ULg

Poster (2015, May)

We analyse the large sample of polarization measurements of the flat-spectrum radio sources of the JVAS/CLASS 8.4-GHz surveys compiled by Jackson et al. (2007). We tested the uniformity of the ... [more ▼]

We analyse the large sample of polarization measurements of the flat-spectrum radio sources of the JVAS/CLASS 8.4-GHz surveys compiled by Jackson et al. (2007). We tested the uniformity of the polarization position angles for a wide range of angular (2D) and comoving (3D) separations and studied the several subsamples, dividing the main sample of 4155 sources regarding their object type (QSO, galaxies, radio sources,...). We found regions of the sky of about 20 degree radius in which quasars (only) have correlated polarization position angles. Those regions coincide with the regions of alignment at optical wavelength pinpointed in 1998 by Hutsemékers. [less ▲]

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See detailUse of 454 pyrosequencing protocol for the assessment of cyanobacterial diversity
Stelmach Pessi, Igor ULg; De Carvalho, Pedro; Laughinghouse, Haywood Dail et al

Poster (2015, May)

A pilot run was carried out on Antarctic microbial mat samples in order to test different protocols for 454 pyrosequencing and bioinformatic analyses. An artificial community was assembled using22 ... [more ▼]

A pilot run was carried out on Antarctic microbial mat samples in order to test different protocols for 454 pyrosequencing and bioinformatic analyses. An artificial community was assembled using22 cyanobacterial strains in two different abundance distributions. Bioinformatic analyses were carried out on the artificial communities using variations of the mothur and UPARSE pipelines. Moreover, different DNA extraction methods, technical replicates and biases arising from direct barcoded primer amplification were assessed using five microbial mat and soil crust samples. A great variation in richness estimates was observed using different bioinformatic pipelines, with UPARSE generating the most consistent results. On the other hand, community structures observed using different DNA extraction protocols were statistically similar. The same was observed for the technical replicates. Moreover, the use of barcoded primers did not influence the observed community structure. [less ▲]

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See detailLife cycle assessment of biobased chemical building blocks made from European waste streams
Groslambert, Sylvie ULg; Léonard, Angélique ULg

Poster (2015, May)

Many drivers exist to persuade EU businesses to embrace the opportunities of the circular economy. However, the challenges faced by companies, particularly among SME’s, in the drive for resource ... [more ▼]

Many drivers exist to persuade EU businesses to embrace the opportunities of the circular economy. However, the challenges faced by companies, particularly among SME’s, in the drive for resource efficiency include access to funding, knowledge and capability, and ability to implement cost-effective technological solutions. It was these factors that inspired the creation of the ReNEW network. ReNEW, ‘Resource innovation Network for European Waste’, is a €5 million project funded by the Interreg IVB North West Europe scheme aiming to increase cooperation between research and business endeavours to create value from waste by optimising novel technologies for extracting materials from waste and their reuse in the supply chain. ReNEW also aims to inform local, national and European policy makers, and to share transnational best practice and improve specific support to meet the innovation needs of the waste sector. Within the frame of this project, several technologies are investigated by partners to improve the valorisation of organic wastes in chemicals such as the production of furfural (C5H4O2 - CAS 98-01-1) by the chemical hydrolysis of contaminated/hazardous woody biomass. [less ▲]

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See detailRegiospecific radiolabeling of Nanofitin on Ni Magnetic Beads with 18F-FBEM and in vivo PET studies
Dammicco, Sylvestre ULg; Lemaire, Christian ULg; Cinier, Mathieu et al

Poster (2015, May)

Nanofitins(NFs) are a small, single chain and cysteine-free protein able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide ... [more ▼]

Nanofitins(NFs) are a small, single chain and cysteine-free protein able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide range of pH (0-13) and temperature (Tm ~80°C). Their extreme stability, low cost and high tolerability for chemical coupling make NFs an interesting alternative to antibodies. The aim of this study was to develop the first synthesis of a radiolabeled NF since no in vivo biodistribution kinetic studies have been published. [less ▲]

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See detailCOMPARISON OF DIABETES CONTROL ONE YEAR AFTER GASTRIC BYPASS AND MAGENSTRASSE AND MILL PROCEDURES
SCHLECK, Michael ULg; KOHNEN, Laurent ULg; DE FLINES, Jenny ULg et al

Poster (2015, May)

Bariatric surgery has become a main therapy of type 2 diabetes in the obese population. The best surgical procedure to achieve diabetes control remains debated. Gastric bypass would be the preferred ... [more ▼]

Bariatric surgery has become a main therapy of type 2 diabetes in the obese population. The best surgical procedure to achieve diabetes control remains debated. Gastric bypass would be the preferred option according to its incretin stimulating potential. We compared in this study gastric bypass surgery to a pure restrictive procedure in terms of diabetes control at 1 year. [less ▲]

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See detailImproving proviral integration site detection with high throughput sequencing
Durkin, Keith ULg; Artesi, Maria ULg; Rosewick, Nicolas et al

Poster (2015, May)

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See detailLIFE CYCLE ASSESSMENT OF ANTHROPIC WATER
Groslambert, Sylvie ULg; Léonard, Angélique ULg

Poster (2015, May)

In its Regional Policy Statement for 2009-2014, the Walloon Government (Belgium) undertakes to make Wallonia a model of good governance. Applying this principle in the field of the environment needs to be ... [more ▼]

In its Regional Policy Statement for 2009-2014, the Walloon Government (Belgium) undertakes to make Wallonia a model of good governance. Applying this principle in the field of the environment needs to be based on a continuous, objective and strict evaluation of the evolution of the state of environmental components (air, water, soil, biodiversity, etc.), the pressures to which they are subjected, the resultant impacts and the measures taken to improve living conditions within our Region. Within this framework, the General Operational Direction of Agriculture, Natural Resources and Environment (DGARNE) realises reports on the state of the environment in Wallonia, in close collaboration with universities and research centres. A chapter is dedicated to the efficient use of water resources, including a life cycle assessment of anthropic water and rain water harvesting. [less ▲]

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See detailPRESPHOTO project - Preservation of microalgae in the BCCM collections
Crahay, Charlotte ULg; Deprez, Karolien; Vanormelingen, Pieter et al

Poster (2015, May)

The implementation of reliable preservation technologies of the biological resources is crucial for the management of the Biological Ressources Centers. PRESPHOTO, a BRAIN.be project, aims to develop and ... [more ▼]

The implementation of reliable preservation technologies of the biological resources is crucial for the management of the Biological Ressources Centers. PRESPHOTO, a BRAIN.be project, aims to develop and optimize new and cost-effective preservation techniques of photosynthetic microalgae (diatoms and cyanobacteria) in the two BCCM collections, BCCM/DCG and BCCM/ULC. This is a critical factor for the future growth and valorisation of these collections. Firstly, the traditional two-step cryopreservation technique will be adapted to photosynthetic microalgae strains. In particular, we will examine the effects of the culture conditions, the type and concentration of cryoprotectants, and the preservation temperature on the survival of microalgae. The encapsulation/dehydration technique as alternative to the two-step cryopreservation method will be also evaluated. Moreover, an independent validation of the developed protocols will be performed by the Culture Collection of Algae and Protozoa (UK) (subcontractor). Secondly, we will perform genome resequencing of selected strains to investigate the genetic changes induced by different cryopreservation techniques. Finally, a high-quality genomic DNA bank will be established and validated. [less ▲]

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See detailStudy of family drawings and self-esteem in institutionalized children
Gallo, Alicia ULg; Simar, Angélique; Blavier, Adelaïde ULg et al

Poster (2015, May)

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See detailStrategies of regioselective radiolabeling of Nanofitin binder for imaging
Goux, Marine ULg; Dammicco, Sylvestre ULg; Becker, Guillaume ULg et al

Poster (2015, May)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as fluorine-18, zirconium-89 or gallium-68. Despite their ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as fluorine-18, zirconium-89 or gallium-68. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the slow clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag fused genetically to a Nanofitin. A specific phosphorylation step, with the alpha subunit of the casein kinase II, generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium or gallium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray [1]. Considering this tag has been created to specifically anchoring protein on zirconium phosphonate-based microarray, we are currently working on a sequence derived from calcium-binding proteins to chelate specifically lanthanides[2]. As described by Pardoux et al.[3], our strategy is to functionalize this sequence with a phosphate nanocluster able to chelate zirconium or gallium. Our first results shown that a mono-phosphorylatable tag phosphorylated in vitro is able to chelate terbium(III) with a lower affinity than the wild type. Considering that terbium(III) is bigger than gallium(III) or zirconium(IV), we can suppose that the cage obtained is too small but suitable for other ions. In order to validate our hypothesis, we have planned to radiolabel those tags and determine their affinity for gallium(III). In order to validate the use of Nanofitin as a potent alternative tool for in vivo imaging, we have made biokinetic studies in mice with PET and MRI imaging. In these studies, we have radiolabelled with fluorine-18 a Nanofitin and we are currently making use of its specific binding to a cell-surface receptor to target a very precise cell population by using an animal model. Once the phosphorylatable tag optimized for regioselective radiolabelling and the Nanofitin targeting validated in an animal model, the next steps will be to combine these two approaches: we will fuse genetically the tag to the specific Nanofitin, radiolabel it with gallium-68 and perform the biokinetic study of this new radiopharmaceutical product. [1] M. Cinier, M. Petit, F. Pecorari, D. R. Talham, B. Bujoli, and C. Tellier, “Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays.,” J. Biol. Inorg. Chem., vol. 17, no. 3, pp. 399–407, Mar. 2012. [2] L. J. Martin, “Development of Lanthanide-Binding Tags (LBTs) as powerful and versatile peptides for use in studies of proteins and protein interactions,” 2008. [3] R. Pardoux, S. Sauge-merle, D. Lemaire, P. Delangle, L. Guilloreau, J. Adriano, and C. Berthomieu, “Modulating Uranium Binding Affinity in Engineered Calmodulin EF-Hand Peptides : Effect of Phosphorylation,” PLoS One, vol. 7, no. 8, 2012. [less ▲]

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Dekkers, Charlotte; DARFOUF, Rajae ULg et al

Poster (2015, May)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 117 strains have been currently confirmed as M. audouinii. Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (64%, p< 0,0001) with a sex-ratio M/F of 1.97. Data concerning the geographic origin of the family have been obtained in only 33,6% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (43,6%) suggesting bias in the data collection. The geographic origin of the remaining group includes several African countries such as Congo (20,61%), Guinea (12,8%) and Burundi (5,12%). The genotypic analysis led to the distinction of 6 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 96 strains, well distributed in different Belgium locations. Two other groups of three strains each were close to the major group but the analysis of the spectral superposition showed some differences between these groups. The two last groups were clearly different from the major group but species identification was confirmed by ITS sequencing. Conclusion The results of the genomic analysis by Diversilab, show that several groups of M. audouinii isolates co-exist in Belgium providing evidence of genetic heterogeneity. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as the age or ethnical origin. ________________________________________ [less ▲]

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See detailThe effects of perinatal exposure to polychlorinated biphenyls on hippocampal neurogenesis
Pinson, Anneline ULg; Parent, Anne-Simone ULg; chatzi, christina et al

Poster (2015, May)

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See detailDerjaguin-Broekhoff-de Boer analysis of adsorption in very disordered mesopores using probabilistic models
Gommes, Cédric ULg

Poster (2015, May)

Our current ability to produce mesoporous materials with ordered morphology has raised fascinating questions about the impact of geometrical disorder on adsorption and desorption states [1]. Many recent ... [more ▼]

Our current ability to produce mesoporous materials with ordered morphology has raised fascinating questions about the impact of geometrical disorder on adsorption and desorption states [1]. Many recent works have investigated the role of mild elements of disorder, such as local constriction or corrugation superimposed to otherwise geometrically ideal cylindrical pores [2,3]. These works have notably shown that elements of disorder may act as nucleation sites and destabilize vapor-like metastable states. The relevance of these perturbation-like results to fundamentally disordered materials, such as gels, is unclear. In particular, do vapor-like metastable states exist at all in this type of very disordered material? In the present communication, we address this question using probabilistic models to investigate the role of disorder. We generalize the classical Gaussian field models of porous materials [4] and use them to analyze adsorption and desorption in the Derjaguin-Broekhof-de Boer approximation. Our approach differs from earlier contributions in that both the adsorbent and the adsorbate are described in terms of probabilities [5]. This enables us to analyze the adsorbate configuration in very disordered solids using a low-dimensional yet realistic configuration space. We notably show that vapor-like metastable states are unlikely in gel-like disordered materials. [1] D.Wallacher, N. Künzner, D. Kovalev, N. Knorr, K. Knorr, Capillary condensation in linear mesopores of different shape, Phys. Rev. Lett. 92 (2004) 195704; [2] B. Coasne, A. Galarneau, F. Di Renzo, R.M.J. Pellenq, Effect of morphological defects on gas adsorption in nanoporous silicas, J. Phys. Chem. C 111 (2007) 15759; [3] C.J. Gommes, Adsorption, capillary bridge formation, and cavitation in SBA-15 corrugated mesopores: A Derjaguin-Broekhoff-de Boer analysis, Langmuir 28 (2012) 5101-5115; [4] R.J. Pellenq, P. levitz, Capillary condensation in a disordered mesoporous medium: A grand canonical Monte Carlo study, Molecular Physics 100 (2002) 2059;[5] C.J. Gommes, A.P. Roberts, in preparation. [less ▲]

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See detailEvaluation of the effectiveness of simulation of cardiac arrhythmias in children
Bragard, Isabelle ULg; Seghaye, Marie-Christine ULg; Baugnon, Thomas et al

Poster (2015, May)

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See detailThe role of sea ice in the carbon cycle of Polar Seas: 1D to 3D modelling
Moreau, S.; Vancoppenolle, M.; Delille, Bruno ULg et al

Poster (2015, May)

Sea ice participates actively in the biogeochemical cycle of carbon of Polar Oceans, yet to which extent is not clear. We investigated the processes that govern sea ice carbon dy- namics in Polar Regions ... [more ▼]

Sea ice participates actively in the biogeochemical cycle of carbon of Polar Oceans, yet to which extent is not clear. We investigated the processes that govern sea ice carbon dy- namics in Polar Regions through 1D to 3D modelling developments. First, we constrained all major physical and biogeochemical processes with respect to CO2 dynamics (carbon- ate chemistry, biological activity, ikaite (CaCO3•6H2O) precipitation and dissolution and ocean-ice-air CO2 fluxes) in a one-dimensional sea ice model. According to our model, the CO2 budget is driven by the CO2 uptake during ice growth and release by brine drainage, whereas other processes such as brine-air CO2 fluxes, despite significant, are secondary. Subsequently, based on these preliminary conclusions, we evaluated the role of sea ice in the carbon dynamics of Polar Oceans by using an ocean-ice coupled Global Earth System Model. Carbon dynamics (e.g. ocean-atmosphere CO2 fluxes) are driven by the contribution of sea ice growth regions in the Arctic Ocean (mainly the Siberian coast) and sea ice melt regions in the Southern Ocean (off the coast of Antarctica). In addition, the production of deep waters is low in the Arctic Ocean but significant in the Southern Ocean. Therefore, sea ice only contributes to the deep water export of carbon in the Southern Ocean. The role of sea ice in the biogeochemical cycle of carbon is significant and its representation by Global Earth System Models should be improved. [less ▲]

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See detailComparison of individuals’ susceptibility to false memory induced by both DRM and misinformation paradigms involving emotional material
Martial, Charlotte ULg; Dehon, Hedwige ULg

Poster (2015, May)

False memories induced by the DRM procedure (“Deese, Roediger and McDermott”; Deese, 1959; Roediger & McDermott, 1995) or the misinformation procedure (in which a person’s recollection of a witnessed ... [more ▼]

False memories induced by the DRM procedure (“Deese, Roediger and McDermott”; Deese, 1959; Roediger & McDermott, 1995) or the misinformation procedure (in which a person’s recollection of a witnessed event is altered after exposure to misinformation about this event; Loftus et al., 1978) are due to errors in source monitoring processes (e.g., Johnson et al., 1993) and one might expect the correlation between these false memories quite positive. However, the few laboratory studies comparing the DRM paradigm and the misinformation paradigm show small (Zhu et al., 2013) or no correlation (Ost et al., 2013) between the false memories elicited by these procedures but these studies vary in terms of methodological details. For instance, false memories from the misinformation procedure involved emotional content while those from the DRM procedure only included neutral materials. This study investigated the relationship between false memories induced by two different paradigms (a DRM task and a misinformation procedure) both involving an emotional material. Participants (N = 154) completed an emotional variant of the DRM (neutral, positive and negative lists) and the misinformation (neutral, positive and negative images) procedures and their performances on both tasks were compared. Although both paradigms reliably induced false memories in participants, our analyses revealed only a marginally weak positive correlation (r = .147, p = .051) between misinformation and DRM false memories using emotional variants. These results support the idea that DRM and misinformation false memories are underpinned by (at least in part) different mechanisms and that the previous mixed results were not due to the specific content of the DRM or the misinformation task used. [less ▲]

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See detailAnatomic features underlying wood density, in 110 rainforest tree species from central Congo basin
de Haulleville, Thalès ULg; Rousseau, Mélissa; Morin-Rivat, Julie ULg et al

Poster (2015, May)

Investigate the influence of fiber thickness and vessel diameter on the wood density in 110 rainforest tree species, and the relationships between wood density, wood water content and shrinking ratio.

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See detailEffects of a walk phase at the warm up onset on physiological and behavioural parameters of ridden horses (Equus caballus)
Jacquot, Marion; Grosjean, Adeline; Emrot, Cassandre et al

Poster (2015, May)

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See detailGeoarchaeology of the ancient city of Utica (Tunisia) and evolution of the palaeoenvironment of the Medjerda delta
Pleuger, Elisa ULg; Abichou, Hakim; Gadhoum, Ahmed et al

Poster (2015, May)

Phoenician Utica remains today largely unknown, as is its role in the Phoenician expansion in the western Mediterranean. Aristotle and Pliny the Elder mention Utica as a maritime and port city and ... [more ▼]

Phoenician Utica remains today largely unknown, as is its role in the Phoenician expansion in the western Mediterranean. Aristotle and Pliny the Elder mention Utica as a maritime and port city and estimate its origin around the 11th c. BC. However, in the present state of research, no archaeological evidence is earlier than the 9th c. BC, and the location of the Phoenician and Roman port infrastructures remains unknown. Today, the ancient city is located on a promontory in the heart of the Medjerda delta, 10 km inland. This project proposes an interdisciplinary effort to understand the Medjerda delta landscape changes during the Holocene. It starts from an archaeological problem and proposes the contribution of geoarchaeology to the understanding of the relationship between ancient societies and their environment. The fluvial palaeoenvironments and sedimentary processes are studied through the mechanical extraction of cores (15-20 m deep) to reach the early Holocene. Selected sediment samples are then studied in laboratory, using different and complementary approaches. The location of port infrastructures will bring initial answers to the question of the foundation of the city. The study of river palaeoenvironments of the Medjerda delta during the Holocene aim at a better understanding of the nature of the settlement, as well as the function of the city of Utica over time. This study will also assess the impact of the ancient city on the environment and understand how the city adapted to the mobility of this Mediterranean delta. Furthermore, the analysis of sedimentary processes causing the filling of the harbour basin will lead to speculation about the causes of the abandonment of the structures and more generally the decline of the city in favor of Carthage. It will also examine whether natural or anthropogenic factors have influenced this deltaic progradation over the centuries. [less ▲]

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See detailBiosensor based on optical fibers
Lismont, Marjorie ULg; Vandewalle, Nicolas ULg; Joris, Bernard ULg et al

Poster (2015, May)

Medical diagnosis and biotechnology progresses are strongly dependent on the development of sensing devices, which, ideally, would allow the reliable detection of very low amounts of biological species in ... [more ▼]

Medical diagnosis and biotechnology progresses are strongly dependent on the development of sensing devices, which, ideally, would allow the reliable detection of very low amounts of biological species in various environments. In addition to these requirements, the detection tools would also be easy to use and would rapidly response. Fluorescent based biosensors fulfil most of these characteristics. In our work, the intersection between two crossed optical fibers is used as the basic unit of an original optical biosensor. As illustrated by figure 1, one optical fiber is used to carry probe molecules and excite fluorescence while the second one is devoted to carry the target species and collect the optical signal arising from the species interacting at the node. The advantages of our set-up over traditional optical sensors are no surface functionalization, use of low amounts of biological species, limitation of the denaturation risk, ease to use and low detection threshold. The developed biosensor is validated on two systems. The first one is a fluorescent calcium indicator, Oregon green 488 BAPTA-2, whose optical emission signal is affected by Ca2+ ions concentration. The second one is based on Rh-Con A and FITC-Dextran complex for which the FRET phenomenon is affected by glucose concentration. In both cases, the results are in agreement with the ones obtained in cuvettes attesting the efficiency of the sensing device. We also show a prototype of a multichannel device composed of multiple crossed optical fibers which are used as species and light carriers. [less ▲]

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See detailPb-based versus Sn-based perovskite solar cells: Toxicity and environmental burden
Babayigit, Aslihan; Dinh Duy Thanh, ULg; Ethirajan, Anitha et al

Poster (2015, May)

Organometal halide perovskites have rapidly evolved into strong contenders to compete with silicon in the quest for low-cost photovoltaics, with their added value being solution-processability. Their ... [more ▼]

Organometal halide perovskites have rapidly evolved into strong contenders to compete with silicon in the quest for low-cost photovoltaics, with their added value being solution-processability. Their primary drawback, however, is that so far the presence of lead (Pb) is required to obtain the highly favorable electro-optical properties of the most successful perovskite crystals such as CH3NH3PbI3. Together with their tendency to degrade under the influence of humidity, and the corresponding disintegration of the unit cell, this implies that Pb compounds can be released into the environment upon failure of a perovskite module. As already known from literature, Pb is a rather toxic element causing irreversible neurological, nephrotic and hepatic damage. Hence, finding a non-harmful alternative metal, exhibiting similar electro-optical characteristics in the resulting perovskite crystal, could be the solution to improve and ultimately commercialize perovskite-based solar cells. Tin (Sn), also being a group IV metal, has been deemed the most appropriate alternative. However, Sn is also enlisted as a harmful chemical. Animal and human volunteer studies have shown that toxicity symptoms like fever, nausea, nephropathy, etc. emerge upon excessive uptake, raising question marks regarding the suitability of Sn as a more environmentally friendly alternative to Pb in perovskite solar cells. This contribution aims to make a first step towards the assessment of the environmental burden of both Pb and Sn based solar cells in the form of a toxicity study. Well-established aquatic model organisms are exposed to the appropriate degradation products, according to well-defined guidelines of the Organization for Economic Co-operation and Development (OECD). This allows a systematic comparison of Sn and Pb-containing decayed compounds regarding their potentially harmful effects on the environment, and sheds light onto the applicability of both corresponding perovskite families in large-scale photovoltaic systems. [less ▲]

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See detailMania following acute decompensation in ornithine transcarbamylase deficiency.
JACQUINET, Adeline ULg; SIRRS, Sandra; MATTMAN, Andre et al

Poster (2015, May)

Background Ornithine transcarbamylase deficiency is the most common urea cycle disorder and presents an X-linked pattern of inheritance. Both males and females may be affected with variation in severity ... [more ▼]

Background Ornithine transcarbamylase deficiency is the most common urea cycle disorder and presents an X-linked pattern of inheritance. Both males and females may be affected with variation in severity and age of onset. Psychiatric symptoms, including episodic psychosis, atypical depression, confusion, erratic behavior or delirium, are possible presentations of late-onset disease. Manic behaviors have previously been reported with hyperammonemia induced by valproic acid. Usually, psychiatric behaviors cease with normalization of ammonia levels. Case report We report a family with two males with confirmed late-onset OTC deficiency (and other adult males with unexplained lethal encephalopathy) due to a missense mutation in OTC (c.119G>A; p.R40H). One 29-year-old male individual, with no psychiatric history, presented hypomanic symptoms with a significant shift from his personality in the days following his first major episode of acute decompensation. Ammonia levels were measured as 245, 30, 11, 106, 20, 38 and 17 micromol/L on days 2, 5, 8, 9, 9, 9, and 10, respectively, following the acute crisis onset. Symptoms of vomiting, confusion, tremors and loss of consciousness stopped at day 5. Hypomanic symptoms were noted from day 5 and were finally controlled with long-acting quetiapine ten days after normalization of serum ammonia levels (daily levels were normal from day 10 to day 21). CT scan of the brain was normal. Conclusion Illustrated by this case report, occurrence of mania secondary to a hyperammonic crisis may be the consequence of dysregulated neurotransmission balance in brain, which can persist after normalization of serum ammonia levels. [less ▲]

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See detailMapping Posidonia oceanica meadows through time A story of precision, evaluation and fragmentation
Abadie, Arnaud ULg; Jousseaume, Matthieu; Lejeune, Pierre et al

Poster (2015, May)

Over the last decades, the interest in mapping Posidonia oceanica beds has increased along with the improvement of the equipment’s precision of data acquisition. In Calvi Bay (Corsica, France) the meadows ... [more ▼]

Over the last decades, the interest in mapping Posidonia oceanica beds has increased along with the improvement of the equipment’s precision of data acquisition. In Calvi Bay (Corsica, France) the meadows cover an area of about 5 km² and are found at a depth ranging from 3 m to 37 m. The availability of three distinct datasets for 1997, 2002 and 2010 allowed to assess changes in the patchiness of the meadows in the bay and to investigate evolution of maps precision through a surface analysis via GIS software. Thus, three maps were elaborated combining aerial photographs and side scan sonar images. The meadows percentage of cover through time was assessed using four bathymetric sections: 0-10 m, 11-20 m, 21-30 m and 31-40 m. Differences in the patchiness of P. oceanica meadows between 1997 and 2010 appear to be moderate (less than 3 %) in the sections 0-10 m and 11-20 m and then greatly increase with depth: 24 % at 21-30 m and 39 % at 31-40 m. This amazing regression seems hardly natural and unlikely given the slight quantity of human activities that can cause damages on the P. oceanica meadows of the Calvi Bay. These results are likely to be mainly due to the improvement of precision and resolution of the aerial photographs (5 m in 1997, 0.8 m in 2002 and 0.5 m in 2010) and sonar images (5 m in 1997, 3 m in 2002 and 0.5 m in 2010). An issue of habitat determination (human vs instrumental) linked with the method adopted for mapping can also cause differences in the percentage of cover. Given the different accuracy among the three maps, the real regression and fragmentation of P. oceanica meadows could be hardly assessed. However, in several areas where the human activities are important, a clear regression or even a disappearance of the meadows has been observed. It is obvious that the last maps are more accurate than the previous ones and, thus, the former can be used for management purpose as well as for study on the patchiness; however, they still keep uncertainty no matter which method is used to create them. [less ▲]

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See detailInvestigation of Mainstream Tobacco Smoke Particulate Phase by HS-SPME-GC×GC-HRTOFMS
Lizak, Radoslaw ULg; Huston-Fulmer, L; Binkley, J et al

Poster (2015, May)

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See detailIn silico screening to predict chondrocyte hypertrophy using a semiquantitative gene network model
Kerkhofs, Johan ULg; Leijten, Jeroen; Luyten, Frank et al

Poster (2015, April 30)

PURPOSE: In development, chondrocyte hypertrophy is a crucial and well-studied step in endochondral ossification. Hypertrophy may also play a role in pathophysiological processes, including osteoarthritis ... [more ▼]

PURPOSE: In development, chondrocyte hypertrophy is a crucial and well-studied step in endochondral ossification. Hypertrophy may also play a role in pathophysiological processes, including osteoarthritis. We employ a computational approach to estimate the effect of individual factors in this complex process. METHODS: We have combined information gleaned from a high number of publications on chondrocyte differentiation into a gene regulatory network of 46 factors and over 150 interactions. This network can estimate the stability of proliferative chondrocytes/permanent cartilage (stable state with SOX9 activity) and hypertrophic chondrocytes (stable state with RUNX2 activity) by employing 2 measures. A first measure is a Monte Carlo analysis that assesses stability in the face of random initial conditions, the second modifies stable states to estimate the sensitivity to perturbation. RESULTS: For each factor, these qualitative measures are calculated in silico under knockout and overexpression conditions and compared to the wild type situation. This enables screening of the effects of all incorporated factors on cartilage homeostasis, differentiation and pathogenesis via the initiation of hypertrophy. Indeed, our gene network analysis indicated multiple candidate genes for the development of osteoarthritis. Factors that amplify the SOX9 attractor basin include TGFβ, PPR, IGF-I, and PKA. The presence of RAS, IHH, GLI2 and FGF is required for the Runx2 stable state. Using a literature study, we corroborated several of the proposed factors. CONCLUSIONS: In silico screening of overexpression and knockout presents a novel strategy to improve bone and cartilage tissue engineering approaches, and can be used to propose a list of putative therapeutic targets for e.g. osteoarthritis. [less ▲]

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Géron, Bénédicte; Dekkers, Charlotte et al

Poster (2015, April 28)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 97 strains have been currently confirmed as M. audouinii. Preliminary analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (84%) with a sex-ratio M/F of 1.95. Data concerning the geographic origin of the family have been obtained in only 45.8% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (77%) suggesting bias in the data collection. The geographic origin of the remaining group (23%) includes several African countries. The genotypic analysis led to the distinction of 3 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 85 strains, well distributed in different Belgium locations. The last group contains only one strain but this strain was significantly different from the two other variants. Conclusion The automated typing DiversiLab® system proved to be an easy and efficient method to investigate the molecular epidemiology of dermatophytes infections. These preliminary results show that, through Belgium, several groups of isolates co-exist for M. audouinii providing evidence of genetic heterogeneity. At this time all epidemiological informations have not yet been assessed while 35 strains of M. audouinii remain to be analysed genotypically to give definitive conclusions. [less ▲]

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See detailEpidemiology and clinical reporting of candidaemia in Belgium : a national prospective study (TANSIR trial)
Trouvé, Charlotte; Blot, Stijn; Hayette, Marie-Pierre ULg et al

Poster (2015, April 26)

Objectives The aim of this multicenter study was to gather epidemiological data on candidemia in the Belgian population. Another goal was to determine the time in real life setting for reporting to the ... [more ▼]

Objectives The aim of this multicenter study was to gather epidemiological data on candidemia in the Belgian population. Another goal was to determine the time in real life setting for reporting to the treating physicians of the species involved and its antifungal susceptibility. Methods Prospective study in 29 Belgian hospitals. From March 1st, 2013 till February 28, 2014 the first Candida isolate from each episode of candidemia was included. Identification and susceptibility testing were performed according to local procedures and isolates were sent to the National Reference Lab with a completed case report form. Species identification was checked by MALDI-TOF mass spectrometry (MS) and ITS sequencing in case no reliable identification was obtained by MS. Antifungal susceptibility testing was performed according to EUCAST guidelines. The total number of patient admissions and hospitalization days during the study period was retrieved from each hospital. Results 341 isolates were retrieved from 325 patients (53.2% male, median age 66 years, range 1-94 years) admitted to the ICU (34.4%), medical wards (30.8%), surgical wards (15.2%), onco-haematology (10.6%), pediatrics (3.0%), neonatology (1.7%) and other wards (4.3%). The mean incidence rate of candidemia was 0.42 per 1,000 admissions (range 0.07 to 1.44) and 0.60 per 10,000 patient days (range from 0.11 to 2.03). Candida albicans was the main cause of candidemia (51.9%), followed by Candida glabrata (26.7%), Candida parapsilosis (9.9%), Candida tropicalis (4.4%), Candida guilliermondii (2.6%), Candida dubliniensis (1.5%), Candida lusitaniae (1.2%), Candida krusei (1.2%) and Candida metapsilosis (0.6%). Overall resistance to fluconazole was 6.7% and to anidulafungin 0.6% (2 C. glabrata isolates were echinocandin resistant). Resistance to amphotericin B was detected in 1 C. tropicalis isolate, all C. albicans, C. glabrata and C. parapsilosis isolates remained susceptible to this drug. Resistance to fluconazole ranged from 3.5% in C. albicans, 8.6% in C. glabrata, 5.6% in C. parapsilosis to 35.7% (5/14 isolates) in C. tropicalis. These five C. tropicalis isolates showed cross resistance to voriconazole and posaconazole. MIC values for caspofungin ranged from <0,016 to >8mg/L, with MIC50 of 0.06mg/L and MIC90 of 0.25mg/L. The median time between blood sampling and positivity of the blood culture bottle was 37h07min (Q1-Q3: 25h42min-54h28min). The median time between blood culture positivity and reporting of isolate identification and susceptibility to the treating physician was 29h58min (Q1-Q3: 23h21min-40h34min) and 59h34min (Q1-Q3: 48h28min-75h20min) respectively. Conclusions A large variation in the incidence of candidemia among Belgian hospitals was observed. Resistance to azole drugs remained low but emerging resistance to these drugs among C. tropicalis was noted. Resistance to echinocandins remains rare in Belgian Candida isolates. These data will be further analyzed in order to evaluate the influence of the identification and susceptibility testing method on the time to report results to the treating physicians. [less ▲]

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See detailEvaluation of a commercially developed semi-automated PCR-surface enhanced raman scattering assay for the detection of Candida species in blood
HAYETTE, Marie-Pierre ULg; WERY, Marie ULg; BOREUX, Raphaël ULg et al

Poster (2015, April 25)

Objectives Microbiological diagnosis of invasive candidiasis is still dependent on culture-based methods. The use of beta-D-glucan antigen detection is included in the EORTC microbiological diagnostic ... [more ▼]

Objectives Microbiological diagnosis of invasive candidiasis is still dependent on culture-based methods. The use of beta-D-glucan antigen detection is included in the EORTC microbiological diagnostic criteria but is rarely available in the clinical labs. On the other hand, PCR-based methods lack standardization. The RenDx Fungiplex® is a new commercially available semi-automated PCR SERS assay designed for the detection of Aspergillus sp. and Candida sp. including the differentiation of resistant strains as C. glabrata, C. krusei and A. terreus. This study was performed for sensitivity and reproducibility testing of the method on 8 different Candida species. Methods The study was conducted on EDTA-blood collected from a healthy donor. Blood samples were spiked with 10 Candida reference strains: C. albicans ATCC 10231, C. albicans NEQAS 1206 and C. albicans NEQAS 2359; C. glabrata ATCC 90030 ; C. krusei ATCC 6258 ; C. tropicalis NEQAS 1036 ; C. guillermondii NEQAS 1035 ; C. parapsilosis ATCC 22019 ; C. lusitaniae NEQAS 1511 and C. dubliniensis IHEM 14280. Spiked samples were diluted at final concentrations ranging from 1 CFU/mL to 1000 CFU/mL. Cultures on Sabouraud dextrose agar were performed in parallel to control yeasts dilutions. DNA extraction was performed by using proteinase K-based method followed by purification on QIAcube automate. The RenDx Fungiplex®kit (Renishaw) was used for the amplification process and the final detection was processed on the SP-1000 sample analyzer. Reproducibility testing was performed on the three C. albicans reference strains by repeating each test 5 times. Results A total of 142 samples were included in the study. A sensitivity of 10 CFU/mL was reached for C. glabrata, C. krusei, C. tropicalis, C. dubliniensis spiked samples while C. lusitaniae and C. tropicalis performed better at 1 CFU/mL. The three tested reference C. albicans strains and C. guillermondii gave the lowest sensitivity (100 CFU/mL). The reproducibility of the assay was 96% Conclusion RenDx Fungiplex®kit allows the detection of the most frequent Candida species responsible for invasive candidiasis in spiked blood samples. The sensitivity of the test is comprised between 10 and 100 CFU/mL for most Candida sp. and reproducibility is very high. This evaluation allows us to consider this commercial kit for inclusion in a clinical study on invasive candidiasis in comparison with non-molecular diagnostic assays. [less ▲]

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See detailStudy of a new splice variant of Neuropilin-1: antagonistic functions in the regulation of cancer progression?
Hendricks, Céline ULg; Janssen, Lauriane; Delcombel, Romain et al

Poster (2015, April 22)

Neuropilin-1 (NRP1) is a transmembrane glycoprotein and a co-receptor for several growth factors, for example some variants of the Vascular Endothelial Growth Factor A (VEGF-A). It largely contributes to ... [more ▼]

Neuropilin-1 (NRP1) is a transmembrane glycoprotein and a co-receptor for several growth factors, for example some variants of the Vascular Endothelial Growth Factor A (VEGF-A). It largely contributes to the regulation of angiogenesis but also to cancer formation. NRP1 can be considered as a proteoglycan as glycosaminoglycans side chains can be added on serine 612. Currently, six splice variants of NRP1 have been described. An additional form was recently identified in our laboratory. Depending upon the cell types, it represents 20-30% of the total amount of NRP1. As compared to the full size NRP1 (NRP1-FS), 7 amino acids are deleted. As the missing sequence is located 2 amino acids downstream of the Ser612 required for glycosaminoglycans addition, this process could be somehow affected and the function of the protein could be modified. The glycosylation of NRP1-FS and -Δ7 was analyzed in different cells overexpressing each isoform. Western blotting analyses suggested that NRP1-Δ7 was less glycosylated than NRP1-FS. Prostate cancer cells (PC3) were engineered to express NRP1-FS or –Δ7 only in the presence of doxycycline. The migration of these cells was analyzed by scratch assay, with or without doxycycline in the medium. As compared to controls and to NRP1-FS-expressing cells, production of NRP1-Δ7 was linked to a reduction of cell migration. A DNA dosage showed that NRP1-FS enhanced cell proliferation, while NRP1-Δ7 reduced it. Tumor growth was assessed in vitro by a culture in soft agar. As compared to control conditions, expression of NRP1-FS by doxycycline increased colonies formation. By contrast, NRP1-Δ7 inhibited colonies number, suggesting an inhibition of tumorigenesis by this variant. As PC3 cells express basal level of endogenous NRP1, this suggests some competitive inhibition of NRP1 functions by NRP1-Δ7. Finally, the function of each variant was investigated in vivo in a model of injection in the flanks of nude mice of PC3 cells conditionally expressing NRP1-FS or -Δ7. As compared to the control, NRP1-FS increased tumor size and weight. By sharp contrast, the expression of NRP1-Δ7 was associated with a reduction of tumorigenicity. Cells with forced expression of NRP1-Δ7 also developed fewer blood vessels as compared to the control. These results suggest that NRP1-Δ7 have an antagonistic action on cancer formation and angiogenesis. [less ▲]

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See detailHeat survival of Clostridium difficile spores in ground meat during cooking process
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Avesani, Véronique et al

Poster (2015, April 21)

Introduction: Clostridium difficile is a spore-forming pathogen considered as a major cause of enteric disease in humans, with faecal-oral route as the primary mode of transmission. However, recent ... [more ▼]

Introduction: Clostridium difficile is a spore-forming pathogen considered as a major cause of enteric disease in humans, with faecal-oral route as the primary mode of transmission. However, recent studies have reported the occurrence of C. difficile in ground meats at retail stores, indicating that foods could be an additional source of infection in the community. Purpose: The objective of this study was to determine the resistance of C. difficile spores in contaminated ground meat during cooking process. Methods: Prior to testing, to obtain spores and to enhance heterogeneity, spores of two different strains were produced in two nutritious broths. C. difficile spores were experimentally inoculated in 45 g of ground meat (beef and pork) in order to obtain a final contamination of 4,500 ufc g-1. Six heating temperatures (70, 75, 80, 85, 90 and 95°C) were chosen. Samples were heating in a water bath with an integrated program for time-temperature. One sample without inoculum was used as control with a temperature probe placed inside. Once the desired temperature was research in the core of the sample, the heat treatment was prolonged for 10 min. Subsequently, all the samples were placed on the chilling room (4°C) before analyse. These experiments were conducted in duplicate with a spore enumeration in triplicate. Results: Heating contaminated ground meat at 70, 75 and 80°C for 10 min was not effective for C. difficile spores inhibition. However, 10 min of heat shock at 80°C was the only temperature that significantly reduced the number of countable colonies. Heat treatment at 85°C (or more) inhibits the germination of both of the strains tested. Significance: Ensure that ground meat, like burgers or sausages, is heated to more than 85°C would be an important measure to reduce the risk of C. difficile food transmission. [less ▲]

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See detailSedimentary impacts of recent moderate earthquakes in different settings in the Western Gulf of Corinth, Greece
Beckers, Arnaud ULg; Mortier, Clément; Beck, Christian et al

Poster (2015, April 21)

11 short gravity cores retrieved in the Western Gulf of Corinth, Greece, allowed identifying event deposits whose age ranges were compared to an updated earthquakes catalogue for the area. 210Pb-derived ... [more ▼]

11 short gravity cores retrieved in the Western Gulf of Corinth, Greece, allowed identifying event deposits whose age ranges were compared to an updated earthquakes catalogue for the area. 210Pb-derived age-depth curves show that the majority of the event deposits may have been triggered by earthquakes. These results show that moderate earthquakes (Mw ~6.0-6.5) may significantly impact different marine settings, from shallow shelves (70-100 m deep) to the basin floor (330 m deep). The deepest coring sites show the best possible record, but one major earthquake is missing and the age of one event deposit does not fit with any known earthquake. More cores are needed to check the spatial extent of each deposit and to validate the absence of record of some earthquakes, like the 1995 Aigion earthquake. [less ▲]

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See detailNovel synthetic pyridyl analogues of CDDO-Im with improved stability and their potential use in cancer prevention
Cao, Martine ULg; Onyango, Evans; Williams, Charlotte et al

Poster (2015, April 20)

Synthetic oleanane triterpenoids are non­cytotoxic, multifunctional drugs with a broad spectrum of applications for prevention and treatment of cancer and for many other chronic diseases. CDDO­Im, 1 ... [more ▼]

Synthetic oleanane triterpenoids are non­cytotoxic, multifunctional drugs with a broad spectrum of applications for prevention and treatment of cancer and for many other chronic diseases. CDDO­Im, 1[2­Cyano­3,12­dioxooleana­1,9(11­dien­28­oyl] imidazole, synthesized more than a decade ago, is one of the most potent triterpenoids known to date with marked antiinflammatory, cytoprotective, antiproliferative, differentiative and proapoptotic activity on various human and murine tumor cell lines. However, pharmacokinetics of CDDO­Im are not optimal. Therefore, three new pyridyl analogues of CDDO­Im, namely CDDO­3P­Im, CDDO­2P­Im and CDDO­4P­Im, have been synthesized and screened for their possible use as chemopreventive or chemotherapeutic drugs. At low nanomolar concentrations, they were equivalent to CDDO­Im for induction of differentiation in U937 leukemia cells and at higher doses they induced apoptosis. As inflammation and oxidative stress contribute to carcinogenesis, we also assessed their cytoprotective potential. The new compounds suppressed inducible nitric oxide synthase expression in RAW264.7 macrophage­like cells and significantly induced heme oxygenase­1 and NADPH quinone reductase mRNA and protein levels in lung cancer cells as well as in various mouse tissues. Most importantly, pharmacokinetic studies performed in vitro in human plasma and in vivo revealed superior stability for each new analogue. While CDDO­Im was almost completely degraded after 30 min (< 12 % of starting material remaining) in human plasma, the new compounds were more stable with > 50 % still detectable. Six hours after gavage, much higher concentrations of the new derivatives were found in mouse liver, lung, pancreas and kidney in contrast to CDDO­Im. Thus, the new pyridyl analogues have better bioavailability, and because of their potent anti­inflammatory activity and improved stability, they will be tested in vivo in relevant carcinogenesis models [less ▲]

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See detailLiposomes entrapping apigenin for the treatment of glioblastoma
Karim, Reatul ULg; Palazzo, Claudio ULg; Dubois, Nadège ULg et al

Poster (2015, April 17)

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See detailGlobal, diffuse and direct irradiances modelling over northwestern Europe using regional climate model MAR : validation and construction of a 30-year climatology
Beaumet, Julien ULg; Doutreloup, Sébastien ULg; Fettweis, Xavier ULg et al

Poster (2015, April 17)

Incoming solar global irradiances are modelled using MAR regional climate model forced by ERA-Interim reanalysis. Global irradiances are decomposed into direct and diffuse using sigmoid model from Ruiz ... [more ▼]

Incoming solar global irradiances are modelled using MAR regional climate model forced by ERA-Interim reanalysis. Global irradiances are decomposed into direct and diffuse using sigmoid model from Ruiz-Arias et al. (2010). Results are validated using data from the European Solar Radiation Atlas for Uccle and Braunschweig weather stations. A 30-year climatology has been built and trends and variability have been analyzed. [less ▲]

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See detailDiurnal and seasonal variability of CO2 fluxes over a degraded Woodland under a Sudanian climate in Northern Benin, West Africa
Ago, Expédit Evariste ULg; Serça, Dominique; Agbossou, Euloge Kossi et al

Poster (2015, April 17)

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See detailGenetic correlations between methane production and milk fatty acid contents of Walloon Holstein cattle throughout the lactation
Vanrobays, Marie-Laure ULg; Vandenplas, Jérémie ULg; Bastin, Catherine ULg et al

Poster (2015, April 16)

Methane (CH4) from ruminal fermentation is the major greenhouse gas produced by dairy cattle which contributes largely to climate change. Production of CH4 also represents losses of gross energy intake ... [more ▼]

Methane (CH4) from ruminal fermentation is the major greenhouse gas produced by dairy cattle which contributes largely to climate change. Production of CH4 also represents losses of gross energy intake. Therefore, there is a growing interest in mitigating these emissions. Acetate and butyrate have common bio-chemical pathways with CH4. Because some milk fatty acids (FA) arise from acetate and butyrate, milk FA are often considered as potential predictors of CH4. However, relationships between these traits remain unclear. Moreover, the evolution of the phenotypic and genetic correlations of CH4 and milk FA across days in milk (DIM) has not been evaluated. The main goal of this study was to estimate genetic correlations between CH4 and milk FA contents throughout the lactation. Calibration equations predicting daily CH4 production (g/d) and milk FA contents (g/100 dL of milk) from milk mid-infrared (MIR) spectra were applied on MIR spectra related to Walloon milk recording. Data included 243,260 test-day records (between 5 and 365 DIM) from 33,850 first-parity Holstein cows collected in 630 herds. Pedigree included 109,975 animals. Bivariate (i.e., CH4 production and one of the FA traits) random regression test-day models were used to estimate genetic parameters of CH4 production and 7 groups of FA contents in milk. Saturated (SFA), short-chain (SCFA), and medium-chain FA (MCFA) showed positive averaged daily genetic correlations with CH4 production (from 0.25 to 0.29). Throughout the lactation, genetic correlations between SCFA and CH4 were low in the beginning of the lactation (0.11 at 5 DIM) and higher at the end of the lactation (0.54 at 365 DIM). Regarding SFA and MCFA, genetic correlations between these groups of FA and CH4 were more stable during the lactation with a slight increase (from 0.23 to 0.31 for SFA and from 0.23 to 0.29 for MCFA, at 5 and 365 DIM respectively). Furthermore, averaged daily genetic correlations between CH4 production and monounsaturated (MUFA), polyunsaturated (PUFA), unsaturated (UFA), and long-chain FA (LCFA) were low (from 0.00 to 0.15). However, these genetic correlations varied across DIM. Genetic correlations between CH4 and MUFA, PUFA, UFA, and LCFA were negative in early lactation (from -0.24 to -0.34 at 5 DIM) and increased afterward to become positive from 15 weeks till the end of the lactation (from 0.14 to 0.25 at 365 DIM). Finally, these results indicate that genetic and, therefore, phenotypic correlations between CH4 production and milk FA vary following lactation stage of the cow, a fact still often ignored when trying to predict CH4 production from FA composition. [less ▲]

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