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The control measures to be applied in case of an ... [more ▼]Foot-and-mouth disease virus (FMDV) is a highly contagious pathogen of cloven-hoofed mammals and one of the biggest concerns for veterinary authorities. The control measures to be applied in case of an outbreak vary in function of the disease-free or disease-enzootic status. Vaccination depends on the prior identification of the involved viral serotype and subtype, it confers an immunity limited to 6 months and it requires between 4 to 7 days to trigger the immune response (i.e. immunity-gap). The use of anti-FMD drugs has been discussed as an alternative or supplementary method to be used in previously FMD-free countries/zones. Such an antiviral treatment could protect against the viral dissemination to fill the gap between vaccination and the rise of a protective immunity. Apart from broad spectrum antiviral agents, such as ribavirin, specific anti-FMDV molecules have been identified in vitro, but none of them has been used in clinical studies involving ruminants or pigs. Next to the anti-FMDV activity, the absence of toxicity and the withdrawal period influencing the food safety, the cost of the treatment would be another important parameter influencing the potential use of an antiviral agent in the control of a FMD outbreak. The aim of this study was to assess the economic impact of using an antiviral in the control of a FMD epizootic in southern Belgium (Walloon Region). This work was based on the results of previous investigations concerning the epidemiological and economic data of a FMD outbreak in Southern Belgium. In the considered scenario, the epizootic was caused by the introduction of an infected cow (during the incubation time) in a beef cattle farm during winter. During the two weeks between the brood cow introduction and the official declaration of the outbreak, animal movements occurred between other beef cattle farms. The economic effects of the epidemic were evaluated taking into account the air-borne transmission of FMDV, the occurrence of animal movements (two scenarios were considered, with a minimum of 2 and a maximum of 17 movements), the presence of bovine and small ruminant farms, as well as pig farms in the protection and surveillance zones around the initial and secondary outbreaks. The wild fauna was not involved in the epidemic. In order to integrate in the above scenario the application of an antiviral agent in the control of the disease, it was assumed that the efficacy of the anti-FMDV drug was proven by reducing viral excretion in infected animals as well as by preventing the infection in animals at risk. Two hypothetical prices were used to introduce in the model the costs related to the administration of the antiviral drug (5€ and 10€ per dose). Furthermore, different strategies of control could be envisaged, such as the administration of the drug to both domestic ruminants and pigs, or depending on the epidemiological role of these species in the FMD transmission and their density in the territory, the administration of the drug to only one of them. Other scenarios could be characterized by the use of the antiviral in the control of the epizootic within the protection and surveillance zones or in only one of them. The costs associated with the use of antivirals in the different proposed scenarios are compared to the costs and socio-economic losses associated with the FMD outbreak and the implementation of control measures. [less ▲]Detailed reference viewed: 14 (3 ULg) Competitive interaction of a synthetic polycation (PDMAEMA) to human platelets and erythrocytesFlebus, Luca ; Lombart, François ; martinez, Lucia et alPoster (2013, October)Detailed reference viewed: 26 (6 ULg) Martian thermospheric temperatures retrieved from SPICAM dayglow measurementsStiepen, Arnaud ; Gérard, Jean-Claude ; Montmessin, Franck et alPoster (2013, October)Detailed reference viewed: 46 (21 ULg) Caractérisation d’un état de référence de l’Eau Blanche pour guider les travaux de restauration écologiquePeeters, Alexandre ; Hallot, Eric ; Van Campenhout, Jean et alPoster (2013, October)Sur l’ensemble de son parcours de 14,5 km à travers la Fagne, l’Eau Blanche a subi d’importants travaux de rectification et de recalibrage, principalement dans les années soixante lors de travaux ... [more ▼]Sur l’ensemble de son parcours de 14,5 km à travers la Fagne, l’Eau Blanche a subi d’importants travaux de rectification et de recalibrage, principalement dans les années soixante lors de travaux d’assainissement agricole. Ces travaux ont eu pour conséquence de banaliser les habitats aquatiques et rivulaires, entrainant des répercussions sur la faune et la flore aquatique. Dans le cadre du projet Walphy, des travaux de restauration de la continuité transversale ont été réalisés entre 2010 et 2011 sur plus de 6 km de linéaire (comprenant l’Eau Blanche et son affluent, le Grand Morby). Avant ces travaux de restauration et de manière à définir l’état de référence à atteindre, une étude des caractéristiques de l’Eau Blanche avant sa rectification a été entreprise. Ainsi, plusieurs données cartographiques ont pu être rassemblées et analysées, telles que des cartes anciennes et des plans terriers accompagnés de profils transversaux et longitudinaux, ainsi qu’un modèle numérique de terrain à haute résolution MNT-LIDAR. Nous avons dés lors pu comparer le tracé de l’Eau Blanche avant et après sa rectification. Par ailleurs, afin de caractériser l’état de référence visé par les travaux de restauration, les données cartographiques et topographiques anciennes ont été utilisées pour calculer les caractéristiques morphométriques du cours d’eau avant la rectification : pente, indice de sinuosité, longueur d’onde, amplitude et rayon de courbure des méandres, largeur à plein bord, rapport largeur/profondeur... [less ▲]Detailed reference viewed: 19 (4 ULg) Marqueurs urinaires témoins de l'exposition de la population liégeoise à différents perturbateurs endocriniensDewalque, Lucas ; PIRARD, Catherine ; DUBOIS, Nathalie et alPoster (2013, October)Detailed reference viewed: 22 (12 ULg) Validation of analytical method for the determination in serum of psychotropic drugs commonly prescribed in Rwanda by HPLC-DADHahirwa, Innocent; Charlier, Corinne ; DENOOZ, Raphael et alPoster (2013, October)Detailed reference viewed: 30 (6 ULg) GAIMS: a powerful gait analysis system satisfying the constraints of clinical routinePierard, Sébastien ; PHAN BA, Remy ; DELVAUX, Valérie et alPoster (2013, October)Detailed reference viewed: 45 (14 ULg) First Evidence of a Volatile Sex Pheromone in Harmonia axyridis (Pallas)Fassotte, Bérénice ; Fischer, Christophe ; Durieux, Delphine et alPoster (2013, October)Until now, no volatile sex pheromone has been highlighted in Coccinellidae but various studies have suggested the existence of such molecules. In the present work, we have sampled volatile organic ... [more ▼]Until now, no volatile sex pheromone has been highlighted in Coccinellidae but various studies have suggested the existence of such molecules. In the present work, we have sampled volatile organic compounds released in the headspace of virgin females in the multicolored Asian ladybeetle, Harmonia axyridis (Pallas), that were either allowed or not to feed on pea aphids (Acyrthosiphon pisum Harris). When fed on aphids, virgin females showed a stereotypical “calling behavior”, commonly associated with the emission of a sex pheromone in several Coleoptera species. Behavioral assays conducted with calling females in a four-arm olfactometer demonstrated that the blend of released volatile compounds was attractive at a distance for males, but not for other females. The headspace of virgin females that were not previously fed with aphids was not attractive for either sex. GC-MS analyses revealed the presence of five compounds in the volatile blend: (–)-β-caryophyllene, β-elemene, methyl-eugenol, α-humulene and α-bulnesene. Subsequently, we have collected and quantified the constituents from the blend over a period of 9 days after exposure to aphids. All five compounds were produced exclusively after feeding virgin females with aphids, and their quantity significantly increased during the whole period of collection. (–)-β-caryophyllene was found to be the major constituent, representing between 80 and 86 % of the total blend. All these results could promote the development of more specific and efficient management methods to manipulate the movements of this invasive ladybeetle and to reduce its negative impacts on biodiversity. [less ▲]Detailed reference viewed: 56 (8 ULg) L’effet des barrages de castors sur le système hydrographiqueDenis, Anne-Cécile ; Petit, François ; Levecq, Yannick et alPoster (2013, October)L’augmentation croissante des populations de castors sur nos rivières ces dernières années n’est pas sans conséquence notamment sur la géomorphologie et l’hydrologie des fonds de vallée. En effet, les ... [more ▼]L’augmentation croissante des populations de castors sur nos rivières ces dernières années n’est pas sans conséquence notamment sur la géomorphologie et l’hydrologie des fonds de vallée. En effet, les barrages construits par les castors constituent des pièges à sédiments susceptibles d’entrainer une incision en aval par déficit en sédiments. La présence de barrages a également pour principal effet de réduire localement la vitesse du courant et de réguler les débits. De plus, les barrages créés par les castors constituent de véritables obstacles à l’écoulement qui peuvent engendrer une modification du tracé du cours d’eau voir du style fluvial. Enfin, en cas de destruction des barrages, le lâcher soudain d’un volume d’eau conséquent peut engendrer un effet « domino » sur les barrages situés en aval entrainant une évacuation importante des sédiments et une incision du cours d’eau. En région Wallonne, il existe plus de 150 barrages de castor. Une analyse récente de leur localisation montre que le castor s’installe principalement sur des rivières ardennaises de dimension modeste mais également sur des rivières de Lorraine et de Famenne. Les barrages sont généralement observés sur des cours d’eau plutôt incisé, en tête de bassin et dans des plaines alluviales plutôt étroites. Différents stades et modes de construction des barrages ont été mis en relation avec la morphologie des rivières. Il en ressort que ces différences impliquent des processus spécifiques de rupture éventuelle de ces barrages. Ces différents aspects ont été plus particulièrement étudiés dans deux rivières ardennaises (la Chavanne et la Lienne). [less ▲]Detailed reference viewed: 67 (20 ULg) Long term culture and characterization of chicken primordial germ cellsTonus, Céline ; Cloquette, Karine; Ectors, Fabien et alPoster (2013, October)Avian primordial germ cells (PGCs) can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]Avian primordial germ cells (PGCs) can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. Moreover, we recently isolate and cultivate a new PGC line from turkey. All PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a progressive drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. At day 6, colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. In order to evaluate the germinal differentiation of cultured PGCs during the gonadal development as well as the germline transmission rate, we established a stably expressing GFP line that was successfully injected in emrbyos. Results are in progress. [less ▲]Detailed reference viewed: 35 (1 ULg) Dosage sérique de médicaments antinéoplasiques et antiparasitaires par UHPLC-DADMISTRETTA, Virginie ; Charlier, Corinne Poster (2013, October)Detailed reference viewed: 17 (3 ULg) Impact of growth rate on the mechanical properties of Douglas-fir grown in Wallonia (Southern Belgium)Pollet, Caroline; Henin, Jean-Marc; Jourez, Benoît et alPoster (2013, October)Timber is traditionally the main purpose of Douglas-fir in Europe. However, some uncertainties remain concerning the sylviculture that should be applied in order to maximise girth growth rate (GR) without ... [more ▼]Timber is traditionally the main purpose of Douglas-fir in Europe. However, some uncertainties remain concerning the sylviculture that should be applied in order to maximise girth growth rate (GR) without affecting the good mechanical properties of the wood. So, the present study aimed at assessing to what extent the GR of Douglas-firs grown in Wallonia influences the mechanical properties of wood clear samples. The modulus of elasticity (E) and static bending strength (σf) were measured according to NF B51-016 and NF B51-008 and are discussed according to the ring width measured on the samples, as well as on the growth rate of the trees and of the stands, for both juvenile and mature wood. This assessment will help to provide sylviculture guidelines for foresters. The experimental material was derived from 11 Douglas-fir stands distributed all over Wallonia, Southern Belgium. At the time of felling, the age of the stands ranged from 41 to 69 years old. In each stand, six trees were felled, their girth varying from 120cm to 179 cm. As a consequence of the sampling methodology the growth rate of the trees ranged from 3 mm to 7 mm. From the 66 butt logs, 274 and 884 clear samples were taken in the juvenile and mature wood respectively. This research evidenced that: - Whatever the GR, the mean values of E and σf in the juvenile wood are 20% lower than those in the mature wood; - At the tree level, the mean E and σf of the samples taken from trees with ring width>5mm are about 10% lower than those of the samples taken from trees with ring width<5mm, whether considering juvenile or mature wood; - At the stand level and regardless to the type of wood (juvenile/mature), the mean E and σf of the samples originating from stands with mean girth increment>3cm/year are respectively 5% and 9% lower than those of the samples originating from stands with mean girth increment <3cm/year. This observation should lead the forest manager to consider carefully sylvicultural practices that would induce a too fast juvenile growth; high growth rate in the mature wood do not appear to be a problem considering the properties studied here. One should however take into account the impact of the growth rate on the singularities of the boards. [less ▲]Detailed reference viewed: 33 (5 ULg) Behavioural study of  chemical  products during vacuum agitated contact dryingBennamoun, Lyes; Léonard, Angélique Poster (2013, October)Detailed reference viewed: 2 (0 ULg) DRAMATIC OSTEONECROSIS OF THE JAW ASSOCIATED WITH ORAL BIPHOSPHONATES TREATMENT AFTER IMPLANT REMOVAL: A CASE REPORTFERNANDEZ AYORA, Alberto ; HERION, Francine ; LAMBERT, France et alPoster (2013, October)BACKGROUND Osteoporosis affects millions of elderly patients. Bisphosphonates represent first-line therapy. Osteonecrosis of the jaws in patients treated with bisphosphonates is mostly associated with ... [more ▼]BACKGROUND Osteoporosis affects millions of elderly patients. Bisphosphonates represent first-line therapy. Osteonecrosis of the jaws in patients treated with bisphosphonates is mostly associated with intravenous bisphosphonates while the incidence associated with oral bisphosphonates is not significant. AIM This report documents a case of bisphosphonate-related osteonecrosis of the jaw (BRONJ) after dental implant removal in an osteoporotic patient treated with alendronate and risedronate for 15 years. MATERIAL AND METHODS An 83-years-old patient with no significant systemic disease underwent implant removal in the first molar left hemimandible position in 2010 due to a peri-implantitis. The surgical procedure was uneventful thought preventive measures were no taken. In June 2011, the patient was referred to our department with a wide bone sequestration of the left mandibular body. A panoramic radiograph showed an increased bone marrow density with bone sequestration. Since then, the patient underwent several courses of antibiotics and conservative therapy, without relief of symptoms. RESULTS Since 2011, the patient has lost 13 teeth in the mandible and the osteonecrosis has disseminated to the entire jaw and the left mandible condyle. The patient has even lost the left hearing. Resective surgery under general anesthesia is not indicated due to the risk because of the age of the patient. CONCLUSION AND CLINICAL IMPLICATIONS Despite the low risk of BRONJ occurrence after implant surgery in oral N-BP users, the fate of dental implants in these patients remains uncertain. Therefore patients at risk must be given a full explanation of the potential risks of implant failure and BRONJ development. Osteonecrosis of the jaws in association with bisphosphonate therapy is a very serious condition with which all dentists should be familiar. Since the treatment of BRONJ is difficult, prevention is the main goal. When the osteonecrosis begins, it can be very painful to the patient, difficult to treat, and can lead to very deleterious sequelae. [less ▲]Detailed reference viewed: 81 (8 ULg) Implementing river restoration taking into account constraints of water supply protection: the case study of the Bocq River at SpontinPeeters, Alexandre ; De le Court, Bernard; Verniers, Gisèle et alPoster (2013, October)In the Bocq basin, eastern tributary of the Meuse, restoration works consist of making 20 obstacles passable to improve the free movement of fish and sediment. This presentation focuses on a particular ... [more ▼]In the Bocq basin, eastern tributary of the Meuse, restoration works consist of making 20 obstacles passable to improve the free movement of fish and sediment. This presentation focuses on a particular case study involving river restoration in a groundwater abstraction zone. In the sixties, a reach of more than 600 meters of the Bocq was completely channelized in order to avoid any risk of contamination of the nearby important drinking water well field area. Ecological quality was consequently impoverished due to the loss of natural habitats (streambed and banks made of concrete and masonry). In addition, the hydraulic conditions (high flow, low depth) make it totally insurmountable for fish. The restoration project started with a 3-year period of consultation with the water abstraction owner to finally reach an agreement on a restoration project taking into account the need of protection of the water abstraction. The project consisted on building rock weirs at regular interval in order to create a succession of 23 steps and pools. Furthermore, various habitats schemes were implemented such as fish shelter, rock berms for aquatic vegetation, and spawning gravel introduction. In addition a small dike and an expansion area for flooding have been completed to protect the water abstraction. This project is being monitored on the basis of geomorphological and ecological analysis. Geomorphological monitoring focuses on the bedload transport with an analysis of flood and the clogging of the gravel layer. Ecological monitoring is based on two indicators (macroinvertebrates and fishes) as well as the analysis of microhabitats. The first results show an improvement after 1 year. [less ▲]Detailed reference viewed: 11 (3 ULg) Yellow Hypergiant /Luminous Blue VariableCox, N. L. J.; Hutsemekers, Damien ; Vamvatira-Nakou, Chloi Poster (2013, October)Hen3-1379 is a yellow hypergiant (YHG) with evidence for episodic non-quiescent mass-loss over in last 400-1000 years in this post-red supergiant (RSG) phase of its evolution. The observational evidence ... [more ▼]Hen3-1379 is a yellow hypergiant (YHG) with evidence for episodic non-quiescent mass-loss over in last 400-1000 years in this post-red supergiant (RSG) phase of its evolution. The observational evidence suggests that though similar to the low-luminosity LBV Wray15-751, it has not yet moved to the hotter phase and is thus pre-LBV. This confirms the scenario of significant mass-loss during the RSG phase. [less ▲]Detailed reference viewed: 20 (10 ULg) Fibroblasts (L929) and osteoblasts (MG-63) behavior within networks differing in fiber densityLombart, François ; Alexandre, Diane; de Bien, Charlotte et alPoster (2013, October)Detailed reference viewed: 18 (7 ULg) Astrospheres of young and old starsCox, N.; Decin, L.; Marle, A.J. et alPoster (2013, October)Detailed reference viewed: 11 (3 ULg) Are bogs reservoirs for emerging disease vectors? Evaluation of Culicoides populations in the Hautes Fagnes Nature Reserve (Belgium)Zimmer, Jean-Yves ; Losson, Bertrand ; Smeets, François et alPoster (2013, October)Several species of Culicoides (Diptera: Ceratopogonidae) biting midges serve as biological vec{ors for the bluetongue virus (BTV) and the recently described Schmallenberg virus in northern Europe. since ... [more ▼]Several species of Culicoides (Diptera: Ceratopogonidae) biting midges serve as biological vec{ors for the bluetongue virus (BTV) and the recently described Schmallenberg virus in northern Europe. since their recent emergence in this part of the continent, these diseases have caused considerable economic losses to the sheep and cattle industries. Much data is nou, âvailable that describe the distribuüon, population dynamics, and feeding habits of these insects. However, little is known regarding the presence of culicoides in unusual habitats such as peaÿ marshes, nor their potential vector capacity. This study evaluated Culicoides biting midges present in the bogs of a Belgian nature reserve compared to those residing at a nearby cattle farm. Culicoides were trapped in 2011 at four different sites (broadleaved and coniferous forested areas, open environments, and at a scientific station) located in the Hautes Fagnes Nature Reserve (Belgium). An additional light trap was operated on a nearby caftle farm. High numbers of biting midges were captured in the marshy area and most of them were Culicoides impunc{atus, a potential vector of BïV and other pâthogens. ln addition, fewer numbers of c. obsoletus/c. scoticus species, C. chiopterus, and C. dewulfi were observed in the bogs compared to the farm. The wet environment and oligotrophic nature of the soil were probably responsible for these changes in the respective populations. A total of 297,808 Culicoides midges belonging to 27 species were identified during this study and 3 of these species (C. sphagnumensis, C. clintoni and C. comosioculatus) were described in Belgium for the first time. [less ▲]Detailed reference viewed: 29 (9 ULg) A basal thunnosaurian from Iraq reveals disparate phylogenetic origins for Cretaceous ichthyosaursNaish, Darren; Fischer, Valentin ; Liston, Jeff et alPoster (2013, October)A new thunnosaurian from the Kurdistan region of Iraq represents the first post-Triassic ichthyosaur from the Middle East. The specimen is an articulated partial skeleton that includes a partial skull ... [more ▼]A new thunnosaurian from the Kurdistan region of Iraq represents the first post-Triassic ichthyosaur from the Middle East. The specimen is an articulated partial skeleton that includes a partial skull, complete left forefin, partial ribcage and anterior section of the vertebral column. Associated palynomorphs uncontroversially date the specimen (preserved on a loose slab of matrix) to the late Hauterivian- Barremian interval of the Early Cretaceous. A posterior projection on the humerus, short and trapezoidal humerus, enlarged intermedium, and trapezoidal cervical and anterior dorsal neural spines represent autapomorphies. Forefin morphology is archaic: the carpals, metacarpals and phalanges form a mosaic similar to that of Triassic-Early Jurassic parvipelvians, accessory digits are absent, and notching is present on the leading edge of the first digit. These and other characters indicate exclusion of the Iraq ichthyosaur from Ophthalmosauridae, the only ichthyosaur clade currently known from the Cretaceous. A phylogenetic analysis of Parvipelvia – the largest yet produced – recovers the new taxon as the sister- taxon of Ichthyosaurus communis, thereby invoking a ghost-lineage of over 60 million years. Inclusion of the new taxon in analyses produced by other authors also resulted in exclusion from Ophthalmosauridae, though relationships with other neoichthyosaurians are less resolved than in our analysis. We conclude that the new taxon represents a highly conservative relict – a member of the Ichthyosaurus lineage – that retained an ‘Early Jurassic’ grade of pectoral anatomy into the Cretaceous. Ophthalmosauridae and members of the Ichthyosaurus lineage therefore both persisted beyond the Jurassic. Clearly, both have highly contrasting evolutionary histories and Cretaceous ichthyosaurs do not all represent members of the same evolutionary radiation. The Iraq ichthyosaur is one of several new taxa originally worked on by the late Robert M. Appleby. An inability to resolve conflicting views on the age of the specimen, combined with other issues, derailed his plans to publish on this specimen during the 1970s. [less ▲]Detailed reference viewed: 51 (3 ULg) Impact of sludge storage duration on its dewatering and drying abilityPambou, Yvon-Bert; Salmon, Thierry ; Fraikin, Laurent et alPoster (2013, October)Detailed reference viewed: 16 (0 ULg) Study of the virulence of serotypes 4 and 9 of African horse sickness virus in two mouse modelsDe la Grandière de Noronha Cotta, Maria Ana ; Zonta, William ; Mauroy, Axel et alPoster (2013, October)African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is ... [more ▼]African horse sickness (AHSV) is an infectious disease caused by a double stranded positive RNA virus which belongs to the family Reoviridae, genus Orbivirus. The virus has nine serotypes and is transmitted by a culicoides biting midge, principally Culicoides imicola. African horse sickness causes severe morbidity and mortality up to 95 % in horses with severe economic losses. The establishment of an experimental model is needed for the investigation of the pathogenesis of this infection. Two mouse models, interferon-α receptor knock-out mice (A129 KO or IFNAR -/-) and immunocompetent mice (A129 WT), were tested. The viruses used for mice inoculations belonged to the two serotypes which caused epidemics in Europe, serotypes 4 and 9. The virus was inoculated by subcutaneous (SC) route and/or by intra-nasal (IN) route. Whole blood samples were taken from each mouse at regular intervals. The organs (liver, spleen, kidney, lung and brain) were taken at the end of the experiment or when the most affected mice were euthanized. All these samples were tested by a qRT-PCR targeting AHSV genome segment 7. Both serotypes of AHSV were detected by qRT-PCR until three weeks post-infection in blood of IFNAR -/- mice and A129 WT mice infected by SC route. Serotype 4 shows a higher peak of viremia than serotype 9. The peak of viremia was measured between day 2 and day 4 post-infection. These results demonstrate the potential of the immunodeficient mouse model for both clinical and biological features. The setting up of this mouse model has developed a tool for efficient in vivo study to characterize the in vivo virulence of this virus, to monitor the evolution of viral populations during in vivo replication cycles and to test the competence or vectorial capacity of indigenous Culicoides. Research supported by the Belgium Federal Public Service, Health, Food Chain Safety and Environment. [less ▲]Detailed reference viewed: 38 (6 ULg) Lignocellulosic biomass pretreatment impact on the extracted lignins chemical structureManara, Panagiota; Zabaniotou, Anastasia; Vanderghem, Caroline et alPoster (2013, October)Detailed reference viewed: 25 (6 ULg) Lignin as a raw material for industrial materials applicationsRichel, Aurore ; Vanderghem, Caroline ; Jacquet, Nicolas et alPoster (2013, October)Native lignin is an abundant biopolymer on earth and represents 15-30% by weight of the lignocellulosic biomass. Lignin is a heterogeneous cross-linked polymer composed of phenylpropane units (guaiacyl ... [more ▼]Native lignin is an abundant biopolymer on earth and represents 15-30% by weight of the lignocellulosic biomass. Lignin is a heterogeneous cross-linked polymer composed of phenylpropane units (guaiacyl, syringyl or p-hydroxyphenyl) linked together by a panel of specific ether or carbon-carbon bonds.[1] Nowadays, large amounts of lignins and lignin-based wastes are available and originate either from the pulp and paper manufacturing or from the production of bioethanol from lignocellulose. [2] Typically, these lignins are dedicated to energetic purposes by combustion. In recent years however, novel axes for high added value applications have emerged and concern, notably, the use of lignin as performance products (e.g. polymer additives, binders) or specialty chemicals (e.g. surface-active agents) for materials applications.[3] Herein, several applications of lignin in materials sciences with industrial issues are presented. A correlation is established between the extraction processes from lignocellulosic materials and the chemical structure and physico-chemical properties of lignins.[4] Our research group has also developed new methodologies for the extraction of highly pure lignins from several starting materials, including food wastes, herbaceous raw materials and wood biomass. These methodologies include microwave-assisted extraction and two-step conventional pretreatment involving steam explosion. These methodologies are described in this presentation. [less ▲]Detailed reference viewed: 38 (10 ULg) Design of novel polyphosphoesters (co)polymers for improving a drug delivery system based on calcium carbonate particlesErgül, Zeynep ; Boury, Frank; Debuigne, Antoine et alPoster (2013, October)Biomedical applications, especially drug delivery systems, often require biodegradable and biocompatible materials that permit safe retention as well as controlled delivery of a drug. CaCO3 particles are ... [more ▼]Biomedical applications, especially drug delivery systems, often require biodegradable and biocompatible materials that permit safe retention as well as controlled delivery of a drug. CaCO3 particles are safe and biodegradable drug carriers that have excellent properties such as low density, high specific surface areas and porosity for drugs microencapsulation. The encapsulation of a model protein within CaCO3 particles has been successfully investigated by Boury and coworkers but the release profiles still need to be improved. In this project, we would like to slow down the kinetics of protein release. One strategy consists in loading the CaCO3 particles with degradable micelles containing the protein/drug in their core. In this perspective, we have developed a novel class of degradable polyphosphoesters (PPE) (co)polymers able to self-assemble into micelles. Note that the degradation products of PPEs, like phosphates, associated with calcium from the inorganic carrier could favor some reconstruction processes like bone regeneration. Pendant carboxylic acid functions were introduced along the backbone of the copolymers in order to increase their affinity for calcium and facilitate their incorporation in the CaCO3 particles. First, an alkynyl-functionalized phospholane monomer, i.e. butynyl phosphate (BYP), was prepared by condensation of 2-chloro-2-oxo-1,3,2-dioxaphospholane and 3-butyn-1-ol (43 % yield), according to a reported procedure. Then, a series of well-defined (Ɖ < 1.2) alkyne-containing PBYP (co)polymers were synthesized by ring opening polymerization using 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) and thiourea (TU) as catalysts[5] (see scheme below). The structures of the PPE (co)polymers were characterized by 1H NMR, 31P NMR and size exclusion chromatography (SEC). Next, carboxylic acid functions were introduced along the PPE backbone by click-type thiol-yne addition of mercaptopropionic acid onto the pendant alkynes. The degradability of the PPEs was investigated at different pH. In the future, the self-assembly behavior of the PPE-based copolymers will be studied as well as their drug loading capacity and ability to accumulate within the CaCO3 particles. In addition, the acid-bearing PPE-based copolymers will be tested as templating agents for the synthesis of CaCO3 particles. [less ▲]Detailed reference viewed: 15 (2 ULg) Impact of sludge storage duration on its dewatering and drying abilityPambou, Yvon-Bert Poster (2013, October)Annual production of sewage sludge in Europe is estimated at more than twelve million tons of dry matter. Use in agriculture and incineration are the main ways of valorization. In this context, sludge ... [more ▼]Annual production of sewage sludge in Europe is estimated at more than twelve million tons of dry matter. Use in agriculture and incineration are the main ways of valorization. In this context, sludge drying appears as an essential step after mechanical dewatering. It reduces the costs of storage and transport, allows the stabilization and the hygienization of sludge while increasing its calorific value. However, sludge is a colloidal system in which particle form a stable suspension in water, making him difficult to be separated from water. The addition of polyelectrolytes chemical is necessary to help the sludge particles to agglomerate into large flocs that can be separate by mechanical dewatering. Before studying experimentally the influence of polymer’s type and dosage on dewatering performances and subsequent drying behaviour, it is necessary to assess the sludge variability during storage duration, because sludge is a highly fermentable material whose properties and composition can rapidly change. [less ▲]Detailed reference viewed: 37 (2 ULg) Relative Contribution of Walking Speed, Ataxia and Gait asymmetry to the Composition of Gait in Multiple SclerosisPHAN BA, Remy ; Pierard, Sébastien ; LOMMERS, Emilie et alPoster (2013, October)Introduction - Objective: Walking speed measured according to the T25FW is the most widely used descriptor of gait in MS clinical research and practice but other dimensions influencing gait variance exist ... [more ▼]Introduction - Objective: Walking speed measured according to the T25FW is the most widely used descriptor of gait in MS clinical research and practice but other dimensions influencing gait variance exist according to alternative gait analysis methods. The relative importance of these different dimensions of gait relatively to its variance is unknown. Methods: We measured the performances of persons with MS and healthy subjects on the T25FW and the Timed 20-Meter Walk (T20MW) performed in tandem with a new gait analysis system (GAIMS). We performed a factorial analysis of variance to underline the main dimensions influencing gait variance and observed their composition. Findings - Conclusion: The main factor influencing gait variance in conventional walk tests is mostly composed of features related to walking speed. Balance, gait asymmetry and variability also participate to this variance but to a lesser extent. The inverse is observed in tests performed in tandem gait. [less ▲]Detailed reference viewed: 58 (11 ULg) Suivi à long terme d’un chantier de protection de berge par techniques végétales : La Berwinne à la FolieHallot, Eric ; Peeters, Alexandre ; Houbrechts, Geoffrey et alPoster (2013, October)Depuis 2000, le Service Public de Wallonie a réalisé plusieurs chantiers de protection de berge ou de réaménagement de cours d’eau par techniques végétales. Parmi ceux-ci, le site de la Berwinne à La ... [more ▼]Depuis 2000, le Service Public de Wallonie a réalisé plusieurs chantiers de protection de berge ou de réaménagement de cours d’eau par techniques végétales. Parmi ceux-ci, le site de la Berwinne à La Folie (Berneau) a fait l’objet d’aménagement au mois de mars 2001. Trois épis de longueur croissante, légèrement orientés vers l’amont, reliés par des peignes latéraux, avaient été installés une vingtaine de mètres à l’amont d’une berge concave dans le but de limiter son recul tout en préservant sa verticalité. Cette berge verticale constituait un habitat écologique très intéressant notamment pour la nidification des hirondelles de rivage. La zone de calme ainsi créée limitait l’érosion mais possédait des vitesses suffisamment élevées en période de hautes eaux pour permette le remaniement des sédiments. Un suivi géomorphologique du site, basé principalement sur des analyses topographiques, hydrologiques et sédimentologies, est réalisé depuis 2000. Les premières observations avaient mis en évidence un réajustement rapide mais localisé du cours d’eau aux aménagements, suivi d’une période de stabilisation entre 2002 et 2004. Par la suite, une réactivation entre 2004 et 2007 était observée. Nous avions conclu qu’un suivi de plus longue durée était indiqué afin de pouvoir quantifier la durée d’adaptation et l’extension réelle de tels aménagements. De nouveaux levés géomorphologiques réalisées en 2013 confirment la nécessité de réaliser des suivis à long terme car ils montrent que l’adaptation de la Berwinne aux aménagements est toujours en cours et concerne un linéaire beaucoup plus important vers l’aval que le seul site d’intervention. [less ▲]Detailed reference viewed: 12 (4 ULg) Impact of sludge storage duration on its dewatering and drying ability Y.B Pambou, T. Salmon, L. Fraikin, M. Crine, and A. LéonardPambou, Yvon-Bert Poster (2013, October)Annual production of sewage sludge in Europe is estimated at more than twelve million tons of dry matter. Use in agriculture and incineration are the main ways of valorization. In this context, sludge ... [more ▼]Annual production of sewage sludge in Europe is estimated at more than twelve million tons of dry matter. Use in agriculture and incineration are the main ways of valorization. In this context, sludge drying appears as an essential step after mechanical dewatering. It reduces the costs of storage and transport, allows the stabilization and the hygienization of sludge while increasing its calorific value. However, sludge is a colloidal system in which particle form a stable suspension in water, making him difficult to be separated from water. The addition of polyelectrolytes chemical is necessary to help the sludge particles to agglomerate into large flocs that can be separate by mechanical dewatering. Before studying experimentally the influence of polymer’s type and dosage on dewatering performances and subsequent drying behaviour, it is necessary to assess the sludge variability during storage duration, because sludge is a highly fermentable material whose properties and composition can rapidly change. [less ▲]Detailed reference viewed: 35 (1 ULg) Évaluation de la qualité hydromorphologique dans le cadre de projet de restauration de cours d’eauRegnier, Maxime; Peeters, Alexandre ; Verniers, GisèlePoster (2013, October)Walphy.beDetailed reference viewed: 13 (2 ULg) Comet dust profiles from PACS images obtained in the framework of the HSSO projectOpitom, Cyrielle ; Bockelée-Morvan, D.; Hutsemekers, Damien et alPoster (2013, October)In the framework of the HssO project the Herschel PACS instrument acquired images of 7 comets between June 2010 and February 2013. Three of these comets have been imaged at several heliocentric distances ... [more ▼]In the framework of the HssO project the Herschel PACS instrument acquired images of 7 comets between June 2010 and February 2013. Three of these comets have been imaged at several heliocentric distances allowing us to follow up the evolution of the dust coma . Radial profiles have been derived for each image. We measured flux densities at 70, 110 and 160 μm in order to determine the comet dust production rate. In some cases, after deconvolution by the instrumental PSF, we might have detected the nucleus signal in the central pixels. [less ▲]Detailed reference viewed: 10 (4 ULg) The Status of Indigenous Village Chicken Production in Democratic Republic of CongoMoula, Nassim ; Do Duc, Luc ; Hornick, Jean-Luc et alPoster (2013, September 30)Detailed reference viewed: 50 (15 ULg) Molecular mechanisms of type I collagen-induced apoptosis in breast carcinoma cellsMaquoi, Erik ; Assent, Delphine ; Foidart, Jean-Michel et alPoster (2013, September 27)Objective: As invading breast carcinoma cells breach the underlying basement membrane, they become confronted with a dense three-dimensional reactive stroma dominated by type I collagen. To develop ... [more ▼]Objective: As invading breast carcinoma cells breach the underlying basement membrane, they become confronted with a dense three-dimensional reactive stroma dominated by type I collagen. To develop metastatic capabilities, invading tumour cells must acquire the capacity to negotiate this hostile microenvironment. By enmeshing cells in a dense fibrillar network, type I collagen acts as a physical barrier for cell migration as well as an endogenous antigrowth signal, partly by inducing apoptosis in epithelial cells. Aberrant cell survival resulting from an acquired resistance toward apoptosis represents a prominent hallmark of cancers. However, the molecular mechanisms implicated in collagen-induced apoptosis remain poorly defined. Here, we investigate the molecular mechanisms by which type I collagen induces apoptosis in breast carcinoma cells and identify MMP-14, a membrane-anchored matrix metalloproteinase, as a key anti-apoptotic factor. Methods: To investigate the induction of apoptosis by collagen, human breast adenocarcinoma MCF-7 cells overexpressing or not MMP-14 were plated on plastic plates or embedded within three dimensional type I collagen gels (Col3D). Cell death was evaluated by measuring cytoplasmic histone-associated DNA fragments (Cell Death Detection ELISA). The percentage of cells with an apoptotic nuclear morphology was also determined. The interactions between cancer cells and Col3D were analyzed by confocal microscopy and the impact of Col3D on the transcriptome of cancer cells was investigated using Illumina HT-12 BeadArrays. Results: When cultured within Col3D gels, MCF-7 cells displayed a round morphology and a cell death characterized by a Z-VAD-FMK-dependent chromatin condensation, nuclear segmentation and oligonucleosomal DNA fragmentation was induced. Transfection of MCF-7 cells with MMP-14 cDNA promoted the interactions between cells and collagen and prevented apoptosis. A transcriptomic analysis revealed that culturing MCF-7 cells within Col3D altered the expression of about 700 genes, irrespective of MMP-14 expression. Col3D modulated the expression of several apoptosis-related genes. Interestingly, MMP-14 activity was sufficient to prevent the Col3D-dependent induction of Bcl2-Interacting Killer (BIK), a pro-apoptotic member of the Bcl-2 family. Conclusions: Our results shed light on the molecular mechanisms by which a collagen-rich microenvironment triggers apoptosis in invading breast cancer cells. Furthermore, we demonstrate that MMP-14 promotes tumour progression by circumventing apoptosis. [less ▲]Detailed reference viewed: 81 (3 ULg) PILOT PROJECT IN CHR-LIEGE: INVITING SIBLINGS OF CHILDREN WITH CANCER TO A CREATIVE AND EXPRESSIVE WEEK-END WITH PSYCHOLOGISTS AND ONCOLOGISTForget, Patricia; Badot, Isabelle; Missotten, Pierre et alPoster (2013, September 27)The siblings week end with the psycho-medical team was highly successful It opens another way to improve the care of the siblings Questionnary forms should be developped in the future to evaluate the real ... [more ▼]The siblings week end with the psycho-medical team was highly successful It opens another way to improve the care of the siblings Questionnary forms should be developped in the future to evaluate the real psychological impact [less ▲]Detailed reference viewed: 29 (3 ULg) Rapid synthesis of highly ordered mesoporous carbons and evaluation of their performances as anodes in Li-ion batteriesLéonard, Alexandre ; Piedboeuf, Marie-Laure ; Pirard, Jean-Paul et alPoster (2013, September 25)Detailed reference viewed: 7 (0 ULg) Decreasing the microporosity of porous carbon xerogels by surface modification: Towards new materials for anodes in Li-ion batteriesLéonard, Alexandre ; Pirard, Jean-Paul ; Job, Nathalie Poster (2013, September 24)Detailed reference viewed: 13 (0 ULg) Resorcinol-Formaldehyde Carbon Xerogels as Anode Material for Lithium-Ion Battery: Synthesis, Grinding and Coating on Current CollectorPiedboeuf, Marie-Laure ; Léonard, Alexandre ; Pirard, Jean-Paul et alPoster (2013, September 24)Rechargeable lithium-ion batteries show great advantages over traditional batteries and are extensively used for consumer electronic devices due to their high energy density and long cycle life. However ... [more ▼]Rechargeable lithium-ion batteries show great advantages over traditional batteries and are extensively used for consumer electronic devices due to their high energy density and long cycle life. However, the improvement of performance of current lithium-ion batteries requires the optimization of the materials used (electrolyte and electrodes). Therefore, tremendous efforts have been dedicated to exploring new materials with high capacity, excellent cycling performance, low cost and high safety features [1-3]. As an example, carbon xerogels are promising candidates in the development of new high performance C-based anode materials for Li-ion batteries, since such carbonaceous materials show very small changes of volume during the charge/discharge process, providing a long cycle life. Nevertheless, hard carbons also exhibit quite high irreversible capacity losses due to their intrinsic high microporosity [4]. To overcome these disadvantages, the structural and textural characteristics need to be carefully controlled. Also, due to the different morphology of these materials compared to graphite, the deposition of carbon xerogels on current collectors needs to be studied in detail. In this work, porous carbon xerogels have been synthesized from Resorcinol-Formaldehyde mixtures by adjusting the pH of the solution in order to obtain different mesopore sizes. Monoliths of carbon xerogels are obtained after drying of the polymer gel and pyrolysis [5]. These monoliths have been ground by two different methods and particle size distributions were measured by granulometry. Mercury intrusion porosimetry and nitrogen adsorption techniques (BET) have been used to characterize the pore texture of the monolithic and the powder materials. Different conditions have been used for the mixing of carbon xerogels with a binder and a solvent to form slurries. The latter have been cast on a copper foil using bar coating with different openings. After evaporation of the solvent, the resulting coatings were analyzed using scanning electron microscopy (SEM) for the morphology and their thickness was monitored by profilometry. First results indicate that the method of grinding has no influence on the final particle size distribution of the powder. The structural features of the carbon xerogels is well preserved for particles down to one micrometer. Nevertheless, a study of grinding duration shows that additional particles with sizes close to that of the porosity of the carbon appear. As a consequence, the grinding conditions were chosen so as to obtain a compromise between particles small enough to realize a coating on a current collector and particles large enough to maintain the carbon gel structural characteristics. References 1) Goodenough J.B., Kim Y. J. Power Sources 2011; 196(16): 6688-6694. 2) Bruce P.G. Solid State Ionics 2008; 179: 752-760. 3) Cairns A. J., Albertus P. Ann. Rev. Chem. Biomol. Eng. 2010; 1: 299-320. 4) Tran T., Yebka B., Song X., Nazri G., Kinoshita K., Curtis D. J. Power Sources 2000; 85: 269-278. 5) Job N., Théry A., Pirard R., Marien J., Kocon L., Rouzaud J., Béguin F., Pirard J. Carbon 2005; 43: 2481-2494. [less ▲]Detailed reference viewed: 54 (12 ULg) Application of the Data-Interpolating Variational Analysis (DIVA) to sea-level anomaly measurements in the Mediterranean SeaTroupin, Charles ; Barth, Alexander ; Beckers, Jean-Marie et alPoster (2013, September 23)In ocean sciences, numerous techniques are available for the spatial interpolation of in situ data. These techniques mainly differ in the mathematical formulation and the numerical efficiency. Among them ... [more ▼]In ocean sciences, numerous techniques are available for the spatial interpolation of in situ data. These techniques mainly differ in the mathematical formulation and the numerical efficiency. Among them, DIVA, which is based on the minimization of a cost function using a finite-element technique (figure 1). The cost function penalizes the departure from observations, the smoothness or regularity of the gridded field and can also include physical constraints. The technique is particularly adapted for the creation of climatologies, which required a large to several regional seas or part of the ocean to generate hydrographic climatologies. Sea-level anomalies (SLA) can be deduced from satellite-borne altimeters. The measurements are characterized by a high spatial resolution along the satellite tracks, but often a large distance between neighbour tracks. This implies the use of simultaneous altimetry missions for the construction of gridded maps. An along-track long wave-length error (correlated noise, e.g. due to orbit, residual tidal correction or inverse barometer errors) also affects the measurement and has to be taken into account in the interpolation. In this work we present the application and adaptation of Diva to the analysis of SLA in the Mediterranean Sea and the production of weekly maps of SLA in this region. Determination of the parameters The two main parameters that determines an analysis with DIVA are the correlation length (L) and the signal-to-noise ratio (SNR). Because of the particular spatial distribution of the measurements, the tools implemented in Diva for the analysis parameter determination tend to underestimate L and overestimate SNR, leading to noisy analysis (the observation constraint dominates the regularity constraint). Some adaptations of the tools are necessary to solve this issue. Numerical cost Because of the large number of observations to be processed (in comparison with in situ measurements on a similar period), the interpolation method employed is expected to be numerically efficient. Improvements in the implementation of Diva further improved the numerical performance of the method, especially thanks to the use of a parallel solver for the matrix inversion. The performance of finite-element mesh generator was also enhanced, so that interpolation of a data set of more than 1 million data points on a 100-by-100 grid can be performed in a few minutes on a personal laptop. Analysis and error field The analysis and error fields obtained over the Mediterranean Sea are compared with the available gridded products from AVISO. Different ways to compute the error field are compared. The impact of the use of multiple missions to prepare the gridded fields is also examined. [less ▲]Detailed reference viewed: 31 (0 ULg) Study of the activities of PBP3/PBP1b from Escherichia coli and the effect of the immediate divisome partners.Leclercq, Sophie; Derouaux, Adeline ; Terrak, Mohammed Poster (2013, September 23)Detailed reference viewed: 11 (8 ULg) Towards the Redefinition of the Meaning of the Landscape of Liège: proposal for a landscape experimentOcchiuto, Rita ; Szanto, Catherine Poster (2013, September 23)Detailed reference viewed: 41 (9 ULg) Analytical study of “La Famille Soler” by Picasso: from the Blue Period to CubismDefeyt, Catherine ; Vekemans, Bart; Vandenabeele, Peter et alPoster (2013, September 23)Detailed reference viewed: 28 (6 ULg) Orthographic learning in adult skilled readersBinamé, Florence ; Poncelet, Martine Poster (2013, September 21)Detailed reference viewed: 22 (2 ULg) Perception du pouvoir entre visiteurs et visités dans la pratique du tourisme dit « ethnique »: Au-delà des oppositions, authentique / simulacreLekane Tsobgou, Dieudonné ; Schmitz, Serge Poster (2013, September 20)La pratique du tourisme est prise sous l'angle d'une confrontation entre les peuples « visiteurs » et les peuples « visités » (Demanget, 2007). L’exotisme culturel des populations autochtones est la ... [more ▼]La pratique du tourisme est prise sous l'angle d'une confrontation entre les peuples « visiteurs » et les peuples « visités » (Demanget, 2007). L’exotisme culturel des populations autochtones est la principale attraction (Harron et al. 1992). Ainsi, une modification de l’équilibre et une rencontre bilatérale semblent plus opaques. Dès lors, les produits authentiques ou les productions instrumentalisées déterminent la position de pouvoir détenus par les visiteurs ou par les visités. Les promoteurs touristiques cherchent avant tout leur gain d’où l’offre parfois des produits issus de simulacre aux visiteurs en mal d’exotisme. Comment illustrer les jeux de pouvoir et de marchandage qui organisent les rencontres plus ou moins authentiques entre visités et touristes ? Les pratiques du tourisme ethnique en Afrique (Cameroun, Madagascar) et en Asie ( Birmanie, Thailande) nous ont servi d'outils d'analyse et d'expérimentation du jeu de pouvoirs entre visités et visiteurs. [less ▲]Detailed reference viewed: 35 (9 ULg) Simultaneous Measurement of Eight Toxic Aldehydes Coming From Polyunsaturated Fatty Acids Degradation in FoodDouny, Caroline ; Bayonnet, Pierre ; Brose, François et alPoster (2013, September 19)Detailed reference viewed: 11 (4 ULg) Study of the estrogenicity of pure compound migrate from plastic food contact materialsSimon, Coraline ; Oghena, M.; Covaci, A. et alPoster (2013, September 19)Detailed reference viewed: 19 (2 ULg) Drug delivery systems based on amphiphilic polyphosphate-copolymersVanslambrouck, Stéphanie ; Clement, Benoît ; Riva, Raphaël et alPoster (2013, September 18)Thanks to their biocompatibility, biodegradability and their structure similar to natural biomacromoleculesn such as nucleic acids, polyphosphates (PPhos) are of prime interest as biomaterials. In ... [more ▼]Thanks to their biocompatibility, biodegradability and their structure similar to natural biomacromoleculesn such as nucleic acids, polyphosphates (PPhos) are of prime interest as biomaterials. In contrast to poly--caprolactone and polylactides, PPhos properties and functionality are easily tuned via the nature of the pendant group of the starting cyclic monomer. For example, by varying the length of the alkyl chain the hydrophobicity of the PPhos can be adjusted. In this work, an efficient organo-catalytic system was developed to synthesize a series of amphiphilic diblock copolymers, i.e. poly(ethylene oxide)-b-polyphosphate (PEO-b-PPhos) by ring-opening polymerization of cyclic phosphates. This novel approach prevents metallic residues to polute the final product, and which is highly desirable when biomedical applications are foreseen. For drug delivery application, the micellization of these novel diblock copolymers in aqueous media was investigated, as well as, encapsulation of an hydrophobic drug. Data on, the influence of the polyphosphate nature of the polymer on drug loading will be presented. [less ▲]Detailed reference viewed: 48 (13 ULg) Nitrous oxide emissions using quantum cascade laser spectrometry over a production crop: preliminary resultsSALERNO, Giovanni ; Aubinet, Marc ; Joly, Joly et alPoster (2013, September 18)Amongst the greenhouse gases, nitrous oxide is recognized as having the greatest greenhouse forcing potential and as being the third in terms of radiative forcing. Agriculture is known to be the major ... [more ▼]Amongst the greenhouse gases, nitrous oxide is recognized as having the greatest greenhouse forcing potential and as being the third in terms of radiative forcing. Agriculture is known to be the major anthropic emitter. This work is part of the FERTECOL project and its objective is to measure the emissions of nitrous oxide by a production crop with an eddy covariance system. The measurements extent over the growth period, cover a large range of climatic conditions and capture peak events associated with fertilization. The measurements, started in April 2013, are carried at the Lonzée Terrestrial Observatory (50°33'5.83"N- 4°44'46.22"E). On this year, the crop is planted with winter wheat. A Quantum Cascade Laser Absorption Spectrometer (QCLAS, GSMA, France) is used to measure nitrous oxide as well as water vapor and carbon dioxide at a frequency of 5 hertz. The fluxes are computed using the Eddy Covariance technique adapting standards quality and corrections procedures set up for CO2 fluxes to N2O. Standard meteorological measurements are performed in parallel. The analysis will present response of the fluxes to environmental variables as well as to fertilization events during the season. [less ▲]Detailed reference viewed: 35 (4 ULg) Elp3 is required for initiation of colon cancerLadang, Aurélie ; Heukamp, L; Buettner, R et alPoster (2013, September 18)Detailed reference viewed: 9 (2 ULg) Cross-linkable acrylate-functionalized polythiophene to increase the stability of photovoltaic performancesOuhib, Farid ; Tomassetti, Mirco ; Manca, Jean et alPoster (2013, September 18)Detailed reference viewed: 21 (4 ULg) Numerical simulation of the electrical characteristics of CIGS/CdS/ZnO solar cell heterostructuresAmand, Julien ; Nguyen, Ngoc Duy Poster (2013, September 17)The electrical characteristics of CIGS-based solar cell heterostructure have been simulated by numerically solving the basic semiconductor equations by means of a finite differences method based on a ... [more ▼]The electrical characteristics of CIGS-based solar cell heterostructure have been simulated by numerically solving the basic semiconductor equations by means of a finite differences method based on a Scharfetter-Gummel discretization scheme. The electric potential, electric field, carrier concentrations, current densities and recombination rates are obtained as function of the space coordinate and the bias voltage. Starting with the analysis of a single absorber layer structure sandwiched between two metal electrodes, we subsequently studied the properties of the CIGS/ZnO pn heterojunction and the influence of the buffer layer thickness in the CIGS/CdS/ZnO on the cell electrical response. A special focus was also given to the influence of grain boundaries in the bulk of CIGS depending on the defects nature and concentration. [less ▲]Detailed reference viewed: 110 (20 ULg) Replication of large-scale epistasis studies: an example on ankylosing spondylitisBessonov, Kyrylo ; Van Steen, Kristel Poster (2013, September 17)Ankylosing spondylitis (AS) is a common form of inflammatory arthritis occurring in approximately 5 out of 1,000 adults of European descent. Recently, the Australo-Anglo-American Spondyloarthritis ... [more ▼]Ankylosing spondylitis (AS) is a common form of inflammatory arthritis occurring in approximately 5 out of 1,000 adults of European descent. Recently, the Australo-Anglo-American Spondyloarthritis Consortium and the WTCCC2 showed that polymorphisms of ERAP1 only affect AS risk in HLA-B27-positive individuals, hereby establishing an interaction between ERAP1 and HLA in the TASC, WTCCC2 and replication datasets [2,5]. We were able to confirm this interaction although using other SNPs. In this study, we use the aforementioned data from WTTCC2 on AS to address unresolved issues when performing large-scale SNP-SNP interaction studies, so as to better guarantee “stable” and “truly replicable” results. These issues are 1) the choice of variable selection method (e.g., of known loci mapping to genes part of know pathways), 2) the choice of SNPs representing a genomic region (e.g., SNPs with modest versus negligible LD between them), 3) the choice of analysis method (e.g., regression-based versus data-reduction (non-parametric) based), 4) different adjustment schemes for lower-order effects (using additive/co-dominant genetic models). We show that even modest changes in 1)-4) may give rise to quite varying epistasis findings for AS, and motivate some “optimal” choices via extensive simulation studies. In this work we rely on a minimal GWAI protocol for genome-wide epistasis detection using SNPs, as developed in our lab [6][9], using the advanced non-parametric Model-Based Multifactor Dimensionality Reduction (MB-MDR) method [1] and an adapted [*] BOolean Operation-based Screening and Testing (BOOST) algorithm [4]. [*] A BOOST [4] like implementation based on the original BOOST algorithm which accounts for missing genotypes [less ▲]Detailed reference viewed: 24 (3 ULg) Characterization of structures with a complex disposition of YBCO coated conductors for magnetic shielding applicationsWera, Laurent ; Fagnard, Jean-François ; Levin, G. A. et alPoster (2013, September 16)An efficient superconducting magnetic shield can be built as an assembly of YBCO 2G coated conductor sections. Each section is milled and placed around a cylindrical support in order to form a joint free ... [more ▼]An efficient superconducting magnetic shield can be built as an assembly of YBCO 2G coated conductor sections. Each section is milled and placed around a cylindrical support in order to form a joint free superconducting loop where persistent currents can flow and provide a strong attenuation of a magnetic field. Our previous works have shown that this assembly is able to shield an axial quasi static (“DC”) magnetic field and that the shielding performances depend on the aspect ratio and the number of layers. The purpose of the present work is to study experimentally the shielding efficiency of several structures with a more complex orientation and position of YBCO coated conductors. Our aim is to design a magnetic shield that would be able to shield a magnetic field directed at any angle with respect to the superconducting loops. Such a structure can be obtained by placing pairs of coated conductors sections along three orthogonal axes. All experiments are carried out at 77K. The structure is subjected to a quasi-static (“DC”) magnetic field. A Hall probe measures the three components of the local magnetic induction inside the assembly as a function of the applied magnetic induction. The shielding efficiency of the structure is characterized as a function of (i) the magnetic field amplitude, (ii) the position of the Hall probe along the three axes, and (iii) the angles between the applied magnetic field and each axis. The experimental results allow us to determine the shielding efficiency in the central part of the new 3-axes structure. Although the shielding efficiency is lowered with respect to that of the traditional 1-axis-coil geometry, measurements at different field orientations allow us to identify the role played by each of the pairs of coils in screening the external magnetic field. [less ▲]Detailed reference viewed: 45 (16 ULg) Class V β Tubulin during the development of the organ of Corti in ratRenauld, Justine ; Thelen, Nicolas ; Johnen, Nicolas et alPoster (2013, September 13)In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is ... [more ▼]In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is composed by two types of cells: sensory hair cells and non-sensory supporting cells. All these cells are distributed according to a specific arrangement along the whole length of the cochlea. A feature of the organ of Corti’s supporting cell is the presence of an abundant cytoskeleton. This one is mainly composed of microtubules, structures make up by tubulin heterodimers. The heterodimers consist of one molecule of α tubulin and one molecule of β tubulin. β tubulin isotypes are highly conserved in evolution and differ by only a few amino acid residues, implying that the isotypes may have functional significance. Organ of Corti’s supporting cells are constituted by class V β-tubulin, a minor mammalian tubulin (Bhattacharya et al., 2008). Moreover, their microtubules are formed by 15 protofilaments instead of the canonical 13, a unique fact among vertebrates (Banerjee et al., 2008). Such a configuration of protofilaments has been observed in C. elegans’ neurons which are responsible for the mechanosensory sense of touch (Bounoutas et al., 2009). It was also shown that these 15 protofilaments microtubules were essential to the proper functioning of these mechanosensory neurons (Bounoutas et al., 2009). Here we present the spatiotemporal localization of class V β-tubulin during the development of the organ of Corti in rats from embryonic day 18 (E18) until P25 (25th postnatal day). For this purpose, we have used immunolabelings on cryosections of whole cochlea. Our preliminary results demonstrate that class V β-tubulin has a unique distribution in the cochlea, being restricted to supporting cells, especially in pillar cells. [less ▲]Detailed reference viewed: 28 (11 ULg) The impact of the salience of fluency in recognition memory in Alzheimer’s diseaseSimon, Jessica ; Bastin, Christine ; SALMON, Eric et alPoster (2013, September 12)Detailed reference viewed: 28 (7 ULg) In vitro and in vivo Characterization of Adult Bone Marrow Neural Crest Stem Cells and their Implication in Hematopoietic SupportCoste, Cécile ; Neirinckx, Virginie ; Manguette, Jérôme et alPoster (2013, September 12)Detailed reference viewed: 11 (1 ULg) Proteomic study of lumbar spinal cord after quadricipital eccentric exerciseLacrosse, Zoé ; Hody, Stéphanie ; Leprince, Pierre et alPoster (2013, September 12)Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” ... [more ▼]Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” (DOMS). DOMS include muscle pain that appears 24 to 72 hours after exercise, but also stiffness, edema and muscle proteins release in plasma as a hallmark of muscle fibers injuries. The only systematic intervention that brings a muscle protection against DOMS is to realize submaximal eccentric contractions with a progressively increased intensity. The mechanism of this protection, called the “Repeated Bout Effect” (RBE), is not understood. However, it is likely explained by cellular, mechanical and neural theories [Scand.J.Med.&Sci.Sports, 13, 88, 2003]. The objective of this study is to better understand which neural signal is released in the muscle synapse and which brings protection by RBE. Male adult mice (C57BL6) were randomly divided into downhill running (DHR), uphill running (UHR) and untrained control (CONT) groups (n=4/group). DHR group is characterized by eccentric contractions of the quadriceps while UHR is concerned by concentric contractions. Running groups performed a warm-up of ten minutes followed by an interval exercise on an inclined treadmill at a velocity of 20cm/s. The latter consisted of running 18 bouts of 5 minutes interspersed with a 2 minutes rest. Lumbar spinal cord was dissected 24h after the race. Nuclear and cytoplasmic proteins were separately extracted and subjected to a 2D-DIGE analysis coupled with mass spectrometry. We do not observe any cytoplasmic protein modification while in the nuclear extract, seven spots were more abundant in eccentric group and four in concentric group in comparison with control group. The mass spectrometry of these proteins reveals that they are implicated in axoplasmic transport. At 24 hours, too few proteins modifications were detected in lumbar spinal cord, maybe as a consequence of a too short period between race and euthanasia. Implication of axoplasmic transport comforts our starting hypothesis that nervous system is able to protect muscle during the RBE by a synthesis and then a synaptic release of molecules modifying the muscle physiology. [less ▲]Detailed reference viewed: 17 (3 ULg) TRAPPIST monitoring of comet C/2012 F6 (Lemmon)Opitom, Cyrielle ; Jehin, Emmanuel ; Manfroid, Jean et alPoster (2013, September 12)Detailed reference viewed: 15 (2 ULg) A water-soluble salt of curcumin (NDS27) inhibits myeloperoxidase and NADPH oxidase activities, two major enzymes of neutrophils.Derochette, Sandrine ; Mouithys-Mickalad, Ange ; Deby-Dupont, Ginette et alPoster (2013, September 11)Neutrophils (PMNs) produce reactive oxygen species (ROS) to kill pathogenic agents. After appropriate stimulation, leading to the activation of protein kinase C (PKC), the cytosolic subunits of the NADPH ... [more ▼]Neutrophils (PMNs) produce reactive oxygen species (ROS) to kill pathogenic agents. After appropriate stimulation, leading to the activation of protein kinase C (PKC), the cytosolic subunits of the NADPH oxidase (Nox2) are phosphorylated and translocated to the membrane flavocytochrome b558, forming the active enzyme which produces superoxide anion (O2●-). From O2●- derives H2O2 used by the PMNs myeloperoxidase (MPO) to form strong oxidant species. Many human and animal pathologies with fatal issue are associated with uncontrolled activation of PMNs. The modulation of enzymes implied in ROS production is thus a primary target to manage excessive inflammatory events. For this purpose, we evaluated the effects of NDS27, a water-soluble salt of curcumin combined with hydroxypropyl-β-cyclodextrin, on the activities of PKC, Nox2 and MPO. PKC activation was determined by western blotting with specific antibodies against phosphorylated PKC in extracts from PMNs after their incubation or not with NDS27. A cell-free assay was used to evaluate the effect of NDS27 before or after the assembly of Nox2 subunits. MPO activity was tested by the SIEFED technique in which NDS27 was pre-incubated with the enzyme and discarded before its activity measurement. An inhibition of PKC phosphorylation and Nox2 activity were observed at respectively 10-4 and 10-5 M of NDS27. The Nox2 inhibition was more pronounced when NDS27 was added before the assembly stimulation, suggesting a direct action of NDS27 on the subunits translocation. NDS27 also dose-dependently decreased the activity of MPO (21 % at 10-5 M), indicating an interaction with the enzyme structure. Our results demonstrated that NDS27 is a potent inhibitor of the two major enzymes responsible for ROS production in PMNs, and also acts on the activation cascade of Nox2. The modulatory effect of NDS27 towards the oxidant activity of PMNs opens therapeutic perspectives to control pathologies with excessive inflammatory reactions. [less ▲]Detailed reference viewed: 54 (7 ULg) The Multicoloured Asian Ladybird, invasive or not in agroecosystems ?Vandereycken, Axel ; Durieux, Delphine ; Fassotte, Bérénice et alPoster (2013, September 10)The Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) is known to thrive principally in shrubby and arboreal habitats. Its occurrence in agroecosystems remains poorly ... [more ▼]The Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) is known to thrive principally in shrubby and arboreal habitats. Its occurrence in agroecosystems remains poorly documented. This study focuses on the occurrence of this exotic species and its seasonal abundance in various field crops. The abundance of adults and larvae of H. axyridis was evaluated over a four-year period, from 2009 to 2012, in four important agronomical crops (wheat, corn, broad bean and potato) in Belgium. A total of 28 aphid predator species were observed including 14 coccinellid species, 13 hoverfly species and one lacewing species. H. axyridis is present and reproduces in all of the four crops studied, with the largest numbers recorded in corn and broad bean crops. In corn, H. axyridis numbers were found to increase over the four inventoried year, reaching 86% of the aphid predators in 2012, while it represented only 15% in 2009. H. axyridis was not always recorded where aphids were abundant, e.g. aphids were abundant on wheat where no H. axyridis were recorded. H. axyridis starts reproducing after the peak in aphid population, suggesting that H. axyridis is able to complete its development by feeding on alternative prey such as larvae and pupae of the same and other species of ladybird and other aphidophagous species. H. axyridis is often considered to be bivoltine but it only completes one generation per year in field crops. The second generation generally develops late in the season in other habitats. Harmonia axyridis is an invasive and an intraguild predator present in high quantities in some specific crops. In these crops, H. axyridis could negatively impact on population of native species due to IGP observed in several other studies. [less ▲]Detailed reference viewed: 29 (9 ULg) Prevalence and Richness of Gastrointestinal Parasites of Capuchins (Cebus albifrons) Interacting with humans in the Ecuadorian AmazonRamirez, William; Martin, Sarah ; Carrillo Bilbao, Gabriel Alberto et alPoster (2013, September 10)Detailed reference viewed: 54 (4 ULg) Relationship between novelty seeking and stimulant effects of ethanol: Is novelty seeking a stable trait?Nyssen, Laura ; Quertemont, Etienne Poster (2013, September 10)Detailed reference viewed: 37 (9 ULg) Assessing the sedative and stimulant effects of alcohol in a balanced placebo designVilenne, Aurélie ; Kreusch, Fanny ; Quertemont, Etienne Poster (2013, September 10)Detailed reference viewed: 24 (7 ULg) Free Group and RecognizabilityRaskin, Julien Poster (2013, September 09)It is well known that recognizability has many algebraic properties. For example, a subset $L$ of the free monoid $\Sigma^*$ is recognizable if and only if there exists a finite monoid $M$, a subset $P ... [more ▼]It is well known that recognizability has many algebraic properties. For example, a subset$L$of the free monoid$\Sigma^*$is recognizable if and only if there exists a finite monoid$M$, a subset$P$of$M$and a morphism$f : \Sigma^* \to M$such that$L = f^{-1}(P)$. These properties allow us to easily define a concept of recognizability in non-free monoids or even in other algebraic structures, such as groups. Our aim is to study the recognizable subsets of the free group$F_X$generated by$X$. A classical construction of the latter shows that it can be seen as a subset of the free monoid$(X \cup X')^*$, where$X'$is a set of formal inverses of elements of$X$, endowed with an ad hoc operation. When$X$is finite, it appears that$F_X$is a recognizable language of this monoid. It is then natural to wonder if there is a link between recognizability in$F_X$and recognizability in$(X \cup X')^*$. We show that every recognizable language of$F_X$is recognizable in$(X \cup X')^*$, and that we can define a class of automata that recognize the recognizable languages of$F_X$. [less ▲]Detailed reference viewed: 24 (16 ULg) Mediating role of alcohol in the relation between social anxiety, depression and cannabis among adolescents.Schmits, Emilie ; Quertemont, Etienne Poster (2013, September 09)Detailed reference viewed: 29 (5 ULg) Morphology and electrical conductivity of poly(propylene)/multi-walled carbon nanotubes nanocomposites foams compatibilized by poly(propylene) -graft-maleic anhydride (PP-g-MA)Tran, Minh Phuong ; Detrembleur, Christophe ; Alexandre, Michaël et alPoster (2013, September 09)The agglomerate of carbon nanotubes (CNTs) in poly(propylene) (PP) matrix often results in low electrical conductivity and poor mechanical properties. In order to improve the dispersion of CNTs, different ... [more ▼]The agglomerate of carbon nanotubes (CNTs) in poly(propylene) (PP) matrix often results in low electrical conductivity and poor mechanical properties. In order to improve the dispersion of CNTs, different amounts of compatibilizer based on poly(propylene-graft-maleic anhydride) (PP-g-MA) were added in the PP matrix. Carbon nanotubes materbatches pre-dispersed at a high loading in the compatibilizer were used to create the samples used in this study. The nanocomposites of PP/PP-g-MA/CNTs were then foamed in supercritical carbon diozide (scCO2) followed by analysis of the foam morphology and the electrical conductivity. The presence of PPgMA did not significantly change the foam morphology, which exhibits good homogeneity and highly uniform closed-cells with penta-heptagonal cell-form. The expansion volume of the foams is not adversely affected by the addition of the compatibilizer; very high expansion volume (around 15 - 25 times) was achieved. The most interesting point is that the PP-PPgMA - 4wt%CNTs foams show a significantly higher electrical conductivity than the uncompatibilized PP-4wt%CNTs at the same volume percent content of CNTs [less ▲]Detailed reference viewed: 20 (3 ULg) Linear formulation of identifying cdes in graphsVandomme, Elise ; Gravier, Sylvain; Parreau, Aline Poster (2013, September 09)Identifying codes were introduced by Karpovsky, Chakrabarty and Levitin in 1998 and can be applied to locate fire in a building using sensors. Buildings are modelled by graphs with rooms as vertices. The ... [more ▼]Identifying codes were introduced by Karpovsky, Chakrabarty and Levitin in 1998 and can be applied to locate fire in a building using sensors. Buildings are modelled by graphs with rooms as vertices. The placement of sensors in the rooms corresponds to choosing a subset of vertices. Finding a sensor-placement such that the location of a fire in one room can be precisely determined is equivalent to constructing an identifying code in the graph. These are dominating sets of vertices for which the closed neighbourhood of each vertex (i.e., the vertex and its neighbours) has a unique intersection with the set. The problem of finding an identifying code has been widely studied. Yet its formulation as an integer linear problem hasn't been much considered. Let G be a graph with vertex set V, to an identifying code$C\subseteq V$of$G\$ correspond weights x_u (x_u is 1 if u belongs to C, otherwise x_u is 0) satisfying the following : for all vertices u,v * the sum of the x_w for w in the closed neighbourhood of u is at least 1 * the sum of the x_w for w in the symmetric difference of the closed neighbourhoods of u and v is at least 1. Of course, it is interesting to find an identifying code with the smallest possible cardinality. But in general this is a NP-hard problem. A way to obtain bounds on the minimal cardinality is to consider the associated linear problem where the weights x_u are fractional. In the case of vertex-transitive graphs, the minimal cardinality for the fractional case can only take two values which depend on the number of vertices, the degree of the graph and the smallest symmetric difference between any two closed neighbourhoods. We show that for an infinite family of graphs the bound is tight and for another another the bound is far too be reached. [less ▲]Detailed reference viewed: 37 (17 ULg) Crown area allometries for surveying blossoming wild cherry with unmanned aerial system (UAS)Lisein, Jonathan ; Michez, Adrien ; Bonnet, Stéphanie et alPoster (2013, September 09)Detailed reference viewed: 64 (19 ULg) Generic results in Denjoy-Carleman classesEsser, Céline Poster (2013, September 09)Denjoy-Carleman classes are spaces of smooth functions which satisfy growth conditions on their derivatives. We distinguish the class of ultradi fferentiable functions of Roumieu type and the class of ... [more ▼]Denjoy-Carleman classes are spaces of smooth functions which satisfy growth conditions on their derivatives. We distinguish the class of ultradi fferentiable functions of Roumieu type and the class of ultradi fferentiable functions of Beurling typ. Endowed with its natural topology, the Beurling class is a Fr échet space. In the poster, we give a condition to have the strict inclusion of a Roumieu class into aBeurling class. We obtain then generic results about the set of functions of a Beurling class which are nowhere in a Roumieu class. Those generic results are obtained from three di fferent points of view: using the Baire category theorem, the notion of prevalence and the notion of lineability. We also study the particular case of Gevrey classes. [less ▲]Detailed reference viewed: 27 (9 ULg) Classification of riparian forest species (individual tree level) using UAV-based Canopy Height Model and multi-temporal orthophotos (Vielsalm, Eastern Belgium)Michez, Adrien ; Lisein, Jonathan ; Toromanoff, François et alPoster (2013, September 09)Introduction : Despite their relatively low area coverage, riparian forests are central landscape features providing several ecosystem services. Nevertheless, they are critically endangered in European ... [more ▼]Introduction : Despite their relatively low area coverage, riparian forests are central landscape features providing several ecosystem services. Nevertheless, they are critically endangered in European countries by human pressures (livestock grazing, land use conflicts, canalizations, waste water, ...) andalso by natural hazards such as the recent black alder (Alnus glutinosa) extensive decline caused by Phytophthora alni. In this study UAV is used to improve the characterization of riparian areas. Riparian forest species are identified at the individual tree level. The health condition of black alder is assessed. For this purpose a computer based approach has been developped, with low needs of specific operator ability or training. Methods : We used the Gatewing X100 to acquire 16 aerial photographs datasets (7 in classic RGB and 9 in RG NIR) during 5 days (form Augustus to October 2012). We processed a CHM in ArcGIS by combining a national Digital Terrain Model with a photogrammetric DSM generated from a single flight photographs dataset with the "MicMac" opensource platform. The 16 orthophotos were computed with Agisoft Photoscan. Based on the CHM and some basic vegetation index (mean NDVI), a classification/segmentation process was developped in eCognition allowing tree crown extraction. An amount of 113 metrics were computed in eCognition for every tree crown object. The metrics were both derived from the CHM raster and spectral information. Metrics were computed by band (object spectral mean and CHM mean, Harralick entropy, Skewness) but also with band combination (Green NDVI and NDVI). A reference dataset was also acquired through a field survey of 624 individual tree positions accurately localized. The health condition of the black alder was recorded during the field survey. A supervised classification algorithm was developed in R (Random Forest package). Results : Several classification trees were assessed trough global accuracy using the Out Of Bag (OOB) error. The best global accuracy (82%) was obtained when distinguishing the black alder (with no regards for health condition during field survey) from the rest of riparian forest objects. The global accuracy tended to decline when other species were added. When separating healthy black alders from those with symptoms, the global accuracy is 77%. Conclusions : Our study highlights the potential of UAV-based multitemporal orthophotos to identify riparian forest species and health conditions at the tree level. Future studies will focus on quick radiometrics corrections. This could improve global accuracy by reducing the variability caused by illumination conditions [less ▲]Detailed reference viewed: 145 (23 ULg) Cannabis and social anxiety in adolescents: second step of a longitudinal studySchmits, Emilie ; Born, Michel ; Glowacz, Fabienne et alPoster (2013, September 05)Detailed reference viewed: 41 (13 ULg) Sensitivity of the CaCO3 content of marine sediments to the kinetic expression of CaCO3 dissolutionSchneider, Birgit; Munhoven, Guy ; Regenberg, AnkePoster (2013, September 05)The preservation of calcite in marine sediments depends on the CaCO3 saturation state of the ambient pore waters and - to a lesser degree - of overlying seawater. A simple sensitivity analysis shows that ... [more ▼]The preservation of calcite in marine sediments depends on the CaCO3 saturation state of the ambient pore waters and - to a lesser degree - of overlying seawater. A simple sensitivity analysis shows that the two common expressions to quantify the saturation of seawater with respect to CaCO3 (ΔCO3 =[CO3 ]-[CO3 ]sat , Ω = [CO3 ]/[CO3 ]sat ), when used for the calculation of the time dependent rate of CaCO3 dissolution either in the water column or in the sediments, yield very different rates of CaCO3 dissolution. With the help of an ocean biogeochemical model it was found that different kinetic expressions for pelagic CaCO3 dissolution in an ocean acidification scenario result in a wide range of surface to mid-depth calcite fluxes in the open ocean, which has strong implications for particle ballasting, the flux of calcite to the sediments and finally the global carbon cycle. In the present study we employ a marine sediment model that is fed by calcite and other biogeochemical fluxes from the ocean biogeochemical model using different realizations of calcite dissolution kinetics under (1) preindustrial and (2) last glacial maximum background climate conditions to assess the model-data agreement of the calcite content of deep sea sediments. A better understanding of the mechanisms driving calcite dissolution in the ocean is important to assess the time scale of calcite compensation, for example during glacial-interglacial cycles, but also in future greenhouse scenarios. [less ▲]Detailed reference viewed: 25 (0 ULg) The experience of chronic illness and psychoptathology across life stories : the case of haemophilic boys and depressive hospitalized adolescentsJadin, Aurore ; Gauthier, Jean-Marie ; Boulard, Aurore Poster (2013, September 04)The aim of this study is to examine and compare how hemophilic adolescents and depressive hospitalized adolescents tell their own stories, how they build their identities in spite of the treatment ... [more ▼]The aim of this study is to examine and compare how hemophilic adolescents and depressive hospitalized adolescents tell their own stories, how they build their identities in spite of the treatment constraints and how they involve themselves in different relationships. During an individual semi-structured interview, based on Mc Adams’ works, each teenager’s narrative was collected. Various measurements were taken. The discursive analysis and software were used to analyze the data base. First results show hemophilic boys speak about their disease spontaneously but they don’t identify only to it while depressive hospitalized adolescents define themselves exclusively by their mental disorder. Another report is that, opposed to depressive hospitalized adolescents, hemophilic boys invest hobbies, relationships with peers’ as much as non depressive adolescents even if those can feel different and sometimes restrained. [less ▲]Detailed reference viewed: 65 (8 ULg) Highly non-classical symmetric states of an N-qubit systemBaguette, Dorian ; Martin, John Poster (2013, September 02)In this work, we consider two measures of non-classicality for pure symmetric N-qubit states : Wehrl entropy (S) and Wehrl participation ratio (R). Measures of non-classicality help to the understanding ... [more ▼]In this work, we consider two measures of non-classicality for pure symmetric N-qubit states : Wehrl entropy (S) and Wehrl participation ratio (R). Measures of non-classicality help to the understanding of the mechanisms responsible for the transition from quantum to classical physics and are usefull in the context of information processing and quantum-enhanced measurements. [less ▲]Detailed reference viewed: 29 (6 ULg) Influence of dipole-dipole interactions on superradianceDamanet, François ; Martin, John Poster (2013, September 02)Superradiance, known as the cooperative spontaneous emission of a directional light pulse by excited atoms placed in vacuum, has recently regained attention in the context of photon localization [1] and ... [more ▼]Superradiance, known as the cooperative spontaneous emission of a directional light pulse by excited atoms placed in vacuum, has recently regained attention in the context of photon localization [1] and single photon cooperative emission [2]. The dissipative dynamics of the atoms is known to depend dramatically on the ratio between the typical inter- atomic distance and the atomic transition wavelength, notably because of dipole-dipole interactions [3]. In this work, we study the effects of these interactions on superradiance as in [4] by solving numerically the corresponding master equation. In particular, by averaging over many realizations of the randomly distributed atomic positions, we show that the decay of the radiated energy pulse height with the intensity of the dipolar coupling follows a power law. [1] E. Ackermans, A. Gero & R. Kaiser, Phys. Rev. Lett. 101, 103602 (2008). [2] R. Friedberg & J. T. Manassah, J. Phys. B 43, 035501 (2010). [3] M. Gross & S. Haroche, Physics reports 93, 301-396 (1982). [4] B. Coffey & R. Friedberg, Phys. Rev. A 17, 1033 (1978). [less ▲]Detailed reference viewed: 57 (6 ULg) Cattle methane fluxes measurement over an intensively grazed grassland using eddy covarianceDumortier, Pierre ; Aubinet, Marc ; Beckers, Yves et alPoster (2013, September)Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions (European Commission, 2009). Recent technological advances in spectroscopy now ... [more ▼]Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions (European Commission, 2009). Recent technological advances in spectroscopy now permit methane flux measurement using eddy covariance. This method has numerous strengths. It can measure fluxes in situ, continuously and across broad areas. This provides information about meadow and cattle emission behaviour throughout the year and across a broad range of climatic conditions. We will present here a one year monitoring of methane exchange between an intensively grazed meadow and the atmosphere obtained using the eddy-covariance method. Methane fluxes exchanged by a grazed meadow were measured continuously since June 2012 at the Dorinne Terrestrial Observatory (50˚ 18’ 44” N; 4˚ 58’ 07” E; 248 m asl.) in Belgium. The site is an intensively pastured meadow of 4.2 ha managed according to the regional common practices where up to 30 Belgian Blue cows are grazing simultaneously. Flux measurements were made with the eddy covariance technique, using a fast CH4 analyzer (Picarro G2311-f) and a sonic anemometer (Campbell Csat3). Carbon dioxide fluxes and various micro-meteorological and soil variables, biomass growth and stocking rate evolution were also measured at the site. Turbulent fluxes were calculated according to standard eddy covariance computation schemes and were filtered for non-stationarity and for low friction velocity (u*) events. During grazing periods, fluxes are dominated by the enteric fermentation source and average 111 nmol m-2 s-1. They are highly variable, probably due to cow movements in and out the measurement footprint and cow digestion rhythm. Despite this spread, a daily emission rhythm is observed with higher emissions during the afternoon. When fluxes are integrated over large periods, methane emissions were found strongly related to cattle stocking rate with a slope of 7.34±0.78 mol CH4 day-1 LSU-1. Further developments are ongoing in order to improve cattle geo-localization through infra-red cameras and individual home-made GPS devices. The two systems will be compared in terms of cost, efficiency and ease of use. During cow-free periods, the methane flux averages 10.5 nmol m-2 s-1 and is highly variable with some production peaks above 100 nmol m-2 s-1. No relation was found between methane fluxes and soil temperature while a weak negative relation was found between methane fluxes and soil humidity. No soil methane absorption has been observed. European Commission. Fifth National Communication from the European Community Under the UN Framework Convention on Climate Change (UNFCCC). Technical Report - 2009 – 038 (2009). [less ▲]Detailed reference viewed: 82 (17 ULg) Multifractality of quantum wave functionsDubertrand, Rémy; Garcia-Mata, Ignacio; Georgeot, Bertrand et alPoster (2013, September)Detailed reference viewed: 25 (1 ULg) HETEROGENOUS CLINICAL AND LABORATORY PRESENTATIONS IN MAD DEFICIENCYBOEMER, François ; SCHOOS, Roland ; ACQUAVIVA, Cécile et alPoster (2013, September)Detailed reference viewed: 13 (3 ULg) The Ganymede aurora …Gérard, Jean-Claude ; Shematovich, Valery; Bisikalo, Dmitry et alPoster (2013, September)In this Report we present the Monte Carlo model for calculation of oxygen UV and IR emissions due to the electron precipitation in the Ganymede polar regions. These techniques will provide column ... [more ▼]In this Report we present the Monte Carlo model for calculation of oxygen UV and IR emissions due to the electron precipitation in the Ganymede polar regions. These techniques will provide column densities of atmospheric species at better than or equal to 1 km spatial resolution, and will constrain the amount of some specific compounds from limb scans and during stellar occultation. This investigation also needs characterization of the vertical temperature profile from ground up to about 400 km altitude with ~5 km vertical resolution as well as mapping of water vapour concentration. This can be performed by multiple water line observations in the 200-600 μm wavelength range. It shall be complemented by ion and neutral mass spectrometry of plasma particles, radio occultations to measure structures of the neutral atmosphere and ionosphere, and plasma wave measurements to constrain plasma density and temperature of the ionosphere. [less ▲]Detailed reference viewed: 24 (0 ULg) The Upper Paleolithic of the Ikh Tulberin Gol (Northern Mongolia): new excavation at the Tolbor 16 siteZwyns, N.; Gladyshev, S.A.; Gunchinsuren, B. et alPoster (2013, September)Detailed reference viewed: 15 (2 ULg) Insulin Sensitivity during Hypothermia in Critically Ill PatientsSah Pri, Azurahisham; Chase, J. Geoffrey; Le Compte, Aaron J. et alPoster (2013, September)Detailed reference viewed: 26 (4 ULg) Mutual inhibition and bistability: two key elements of the endochondral ossification processGermain, Morgan Poster (2013, September)Detailed reference viewed: 28 (6 ULg) Effect of chronic confinement on sperm quality and male reproductive physiology in Eurasian perch, Perca fluviatilis.Rougeot, Carole ; Tomson, Thomas; Ponthier, Jérôme et alPoster (2013, September)Detailed reference viewed: 51 (7 ULg) Hepatitis E virus infection in wild boars and humans in BelgiumThiry, Damien ; Mauroy, Axel ; Saegerman, Claude et alPoster (2013, September)Detailed reference viewed: 8 (0 ULg) A re-evaluation of species diversity within the Labeo (Cypriniformes: Cyprinidae) with papillary lips from the Congo basinGajdzik, Laura ; Van Steenberge, Maarten; Chilala, Alex et alPoster (2013, September)Detailed reference viewed: 7 (1 ULg) Belgian Register and Reference Centres for Gestational Trophoblastic DiseasesTIMMERMANS, Marie ; DELCOMINETTE, Sarah ; DELBECQUE, Katty et alPoster (2013, September)Detailed reference viewed: 19 (6 ULg) Magnetic hysteresis cycle and remnant field distribution of bulk high temperature superconductor / ferromagnet hybridsPhilippe, Matthieu ; Fagnard, Jean-François ; Wera, Laurent et alPoster (2013, September)Bulk (RE)BCO materials can be used potentially as powerful permanent magnets. The magnetic flux distribution above a bulk HTS magnet, however, is strongly non-uniform (conic profile predicted by the Bean ... [more ▼]Bulk (RE)BCO materials can be used potentially as powerful permanent magnets. The magnetic flux distribution above a bulk HTS magnet, however, is strongly non-uniform (conic profile predicted by the Bean model) compared to the rather flat distribution above a ferromagnet. In the present work, we study how FeNi soft ferromagnetic alloys of different shapes can be combined with a bulk, large grain (RE)BCO superconductor (RE denotes a rare-earth element) to improve the distribution of trapped field at the surface or its average value through the volume of the sample. A FeNi ferromagnetic alloy was machined into pieces of various shapes (cylinders and rings) and attached to (i) the top surface of the bulk HTS cylinder to form bulk ferromagnet / superconductor (F/S) hybrids and (ii) to the top and bottom surfaces to form bulk F/S/F hybrids. The magnetic properties of each hybrid structure were measured under axial magnetic field at 77 K. Pick-up coils wound around the superconductor were used to measure the average magnetic induction inside the superconductor while the remnant induction distribution near the top and bottom surfaces was determined by miniature Hall probe mapping. The modifications of the hysteresis curves and flux distributions were analyzed by taking into account the ferromagnet intrinsic properties (intrinsic permeability, saturation) and geometrical properties (shape, size and volume). The results show that the effect of the ferromagnet increases with its volume. In presence of a ferromagnet, the superconductor hysteresis curve shows a combination of a diamagnetic and a ferromagnetic behaviour on which it is worth noting that (i) the bulk remnant magnetization increases and (ii) in the magnetic saturation regime of the ferromagnet, the magnetic effects of the superconductor and the ferromagnet are superimposed. The results also give evidence that flux lines curve through the ferromagnetic component, which produces a decrease of the self-demagnetizing field inside the superconductor. [less ▲]Detailed reference viewed: 23 (5 ULg) Optimal conception of a post-combustion CO2 capture unit with assessment of solvent degradationLéonard, Grégoire ; Toye, Dominique ; Heyen, Georges Poster (2013, September)Solvent degradation may be a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. A kinetics model describing ... [more ▼]Solvent degradation may be a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. A kinetics model describing solvent oxidative and thermal degradation has been developed based on experimental results. This model has been included into a global Aspen Plus model of the CO2 capture process, so that optimal operating conditions can be identified to minimize both energy and environmental impacts of the process. [less ▲]Detailed reference viewed: 47 (18 ULg) PIXE Analysis for the pigment identification in the Nizet manucript (18th century)Machowski, Mélanie ; Calvo Del Castillo, Helena ; Oger, Cécile et alPoster (2013, September)Written in 1740, the Nizet Manuscript is a heraldry book compiling the genealogy of the Nizet family (Verviers, Belgium). It presents a large number of hand-painted heraldries in traditional heraldic ... [more ▼]Written in 1740, the Nizet Manuscript is a heraldry book compiling the genealogy of the Nizet family (Verviers, Belgium). It presents a large number of hand-painted heraldries in traditional heraldic colours; the gold- and silver-like colours have undergone alteration and induced the degradation of the paper. The first inspection of the book with a binocular lens and UV-visible spectroscopy has led to the selection of representative points to be studied by PIXE analysis for the different groups of pigments. The PIXE measurements have been conducted with the cyclotron of the Institute of Nuclear and Atomic Physics and of Spectrometry of the University of Liege. Some pigments have been clearly identified, such as vermillion, umber or brass, while the presence of some others needs to be confirmed, for example azurite or lapis-lazuli. In the case of the green pigments and the organic ones (red, pink and black colours), the PIXE results must be completed by a molecular analysis. In order to exactly identify all the pigments, Raman analyses will be done to complete the pigment identification of the Nizet Manuscript. [less ▲]Detailed reference viewed: 53 (17 ULg) X-ray emission of interacting wind binaries in Cyg OB2Nazé, Yaël ; Cazorla, Constantin ; Rauw, Grégor Poster (2013, September)Cyg OB2 #5, #8A, and #9 are binary or multiple massive stars in the Cyg OB2 association displaying several peculiarities, such as bright X-ray emission and non-thermal radio emission. Our X-ray monitoring ... [more ▼]Cyg OB2 #5, #8A, and #9 are binary or multiple massive stars in the Cyg OB2 association displaying several peculiarities, such as bright X-ray emission and non-thermal radio emission. Our X-ray monitoring of these stars reveals the details of their behaviours at high energies, which can be directly linked to wind-wind collisions (WWCs). In addition, the X-ray emission of Cyg OB2 #12, an evolved massive star, shows a long-term decrease, which could hint at the presence of a companion (with associated colliding winds) or indicate the return to quiescence of the system following a recent eruption. [less ▲]Detailed reference viewed: 58 (21 ULg) Interactions of two aphid species on the african eggplant, sorrel and amaranthBayendi-Loudit, Sandrine ; Chevalier Mendes Lopes, Thomas ; Francis, Frédéric Poster (2013, September)Aphids (Homoptera: Aphididae) are polyphagous insects which can be found on several crops in temperate zones, as well as in the tropics. The multiplication of Aphis gossypii Glover (C9 cucumber, Burk ... [more ▼]Aphids (Homoptera: Aphididae) are polyphagous insects which can be found on several crops in temperate zones, as well as in the tropics. The multiplication of Aphis gossypii Glover (C9 cucumber, Burk cotton and Pipo pepper strains) and Myzus persicae Sulzer was studied in the laboratory on three plant species: African eggplant, Solanum aethiopicum, sorrel, Hibiscus sabdariffa, and amaranth, Amaranthus spp. Periodic counts were carried out to monitor population growth. The multiplication rate of M. persicae wass higher than that of A. gossypii when these two species were present together on eggplant. Amaranth was less suitable for the development of both species, but Myzus persicae again had a better multiplication rate than Aphis gossypii. This study illustrated the importance of considering not only one pest species, but the whole herbivore guild, especially when biological control is important. [less ▲]Detailed reference viewed: 18 (4 ULg) Local time variations of the main auroral emission at JupiterPalmaerts, Benjamin ; Radioti, Aikaterini ; Grodent, Denis et alPoster (2013, September)Detailed reference viewed: 29 (12 ULg) Outcomes of women with endometrial cancer after introducing a robotics programLAMBERT, Marie ; TIMMERMANS, Marie ; DELBECQUE, Katty et alPoster (2013, September)Detailed reference viewed: 19 (8 ULg) Why Would I Use Commercial Sharing Programs? A Realistic View of Consumer (Non-) Usage IntentionsHazee, Simon Poster (2013, September)Detailed reference viewed: 36 (4 ULg) How can long-term experimental plots can help us to understand the sustainability of different phosphorus inputs ?Renneson, Malorie ; Dufey, Joseph; Roisin, Christian et alPoster (2013, September)Detailed reference viewed: 32 (10 ULg) Application of a Design Space Approach for a Pharmaceutical Tablet Formulation Optimization based on a Mixture Design of Experiments and using Vibrational Spectroscopy as PAT ToolChavez, Pierre-François ; Netchacovitch, Lauranne ; Sacre, Pierre-Yves et alPoster (2013, September)Detailed reference viewed: 41 (14 ULg) Mouvements de masses et analyse morphostructurale dans le Constantinois - Algérie nord-orientaleDraidia, Salah ; Havenith, Hans-Balder ; benabbas, ChaoukiPoster (2013, September)Detailed reference viewed: 30 (5 ULg) First report of Mortierella wolfii causing human diseaseLAYIOS, Nathalie ; HAYETTE, Marie-Pierre ; HUWART, Aline et alPoster (2013, September)Detailed reference viewed: 16 (2 ULg) Negative effect of milk production level on reproduction performances. Not a fatality.Hanzen, Christian ; Glorieux, Géry; Chapaux, PhilippePoster (2013, September)Detailed reference viewed: 21 (5 ULg) TiO2 templated films used as photoelectrode for solid-state DSSC applications: study of the pore filling by Rutherford Backscattering SpectroscopyDewalque, Jennifer ; Colson, Pierre ; Thalluri, Venkata Visveswara Gopala Kris et alPoster (2013, September)Liquid-state dye-sensitized solar cells can suffer from electrolyte evaporation and leakage. Therefore solid-state hole transporting materials are investigated as alternative electrolyte materials ... [more ▼]Liquid-state dye-sensitized solar cells can suffer from electrolyte evaporation and leakage. Therefore solid-state hole transporting materials are investigated as alternative electrolyte materials. However, in solid-state dye-sensitized solar cells, optimal TiO2 films thickness is limited to a few microns allowing the adsorption of only a low quantity of photoactive dye and thus leading to poor light harvesting and low conversion efficiency. In order to overcome this limitation, high surface area templated films are investigated as alternative to nanocrystalline films prepared by doctor-blade or screen-printing. Moreover, templating is expected to improve the pore accessibility what would promote the solid electrolyte penetration inside the porous network, making possible efficient charge transfers. In this study, films prepared from different structuring agents are discussed in terms of microstructural properties (porosity, crystallinity) as well as effect on the dye loading and Spiro-OMeTAD (2,2',7,7'-tetrakis-(N,N-di-p-methoxyphenylamine)9,9'-spirobifluorene) solid electrolyte filling. Different techniques such as transmission electron microscopy (TEM), atmospheric poroellipsometry (AEP) and UV-visible absorption spectroscopy (UV-vis.) have been used to describe the microstructural features of the films. Besides, we have implemented Rutherford backscattering spectrometry (RBS) as an innovative non-destructive tool to characterize the hole transporting materials infiltration. Templated films show dye loading more than two times higher than nanocrystalline films prepared by doctor-blade or screen-printing and solid electrolyte infiltration up to 88%. [less ▲]Detailed reference viewed: 93 (19 ULg) Disruption in energy metabolism and mitochondrial function in a cellular model of inflammation-induced acute kidney injuryQuoilin, Caroline ; Mouithys-Mickalad, Ange ; Lécart, Sandrine et alPoster (2013, September)Sepsis is a very complex clinical condition characterized by stimulation of a systemic inflammatory response due to an infection. It has a profound deleterious effect on kidney functions leading to sepsis ... [more ▼]Sepsis is a very complex clinical condition characterized by stimulation of a systemic inflammatory response due to an infection. It has a profound deleterious effect on kidney functions leading to sepsis-induced acute kidney injury (AKI). This failure seems to occur through complex mechanisms involving the immune system response, inflammatory pathways, cellular dysfunction and hemodynamic instability. To study the role of cellular energetic metabolism dysfunction and mitochondrial impairment in the occurrence of AKI during sepsis, we developed an inflammation-induced in vitro model using proximal tubular epithelial cells (HK-2) exposed to a bacterial endotoxin (lipopolysaccharide, LPS). This investigation has provided key features on the relationship between endotoxic stress and mitochondrial respiratory chain assembly defects. Firstly, we have shown that renal cells subjected to LPS are no longer capable to use adequately the available oxygen to maintain their metabolic functions. One hypothesis of this down-regulation suggests that impairment in mitochondria oxidative phosphorylation could prevent cells from using oxygen for adenosine triphosphate (ATP) production and potentially could cause sepsis-induced organ failure. Our study has then investigated this possible mitochondrial impairment to explain the decreased O2 consumption rate observed in LPS-treated HK-2 cells. After exposure to LPS, functionality of mitochondria was affected without any disturbance in their spatial organization. LPS seemed rather to interrupt mitochondrial oxidative phosphorylation by blocking cytochrome c oxidase activity. As a consequence, disruptions in the electron transport and the proton pumping across the system occurred, leading to a decrease of the mitochondrial membrane potential, an electron leakage as the form of superoxide anion, a release of cytochrome c in the cytosol and a decrease in ATP production. This irreversible defect in the production of cellular energy would support the concept that kidney failure in sepsis may occur on the basis of cytopathic hypoxia. [less ▲]Detailed reference viewed: 38 (6 ULg)