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See detailUse of visible-near infrared spectroscopy to determine cheese properties
Troch, Thibault ULg; Vanden Bossche, Sandrine; De Bisschop, Céline et al

Poster (2013, August)

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See detailAssessment and monitoring of forest-game balance: an exclosure experiment
Lehaire, François ULg; Licoppe, Alain ULg; Lejeune, Philippe ULg

Poster (2013, August)

During the last decades, populations of large ungulates have largely increased, strengthening the pressure exerted by these species on forest vegetation. Therefore, monitoring this pressure has become ... [more ▼]

During the last decades, populations of large ungulates have largely increased, strengthening the pressure exerted by these species on forest vegetation. Therefore, monitoring this pressure has become unavoidable for sustainable forest management. Such monitoring requires a rigorous approach in order to evaluate objectively the balance between game population and forestry. The use of exclosure experiment offers an interesting solution to observe the effects of game populations on forest ecosystem. When objectives expected from forest management are clearly defined, exclosure experiments can effectively be used as a monitoring tool, to allow detecting unbalanced situations, for example, herbivore pressure threatening forest regeneration. The monitoring tool combines on one side an exclosure, defined as "the real environment", fully accessible to herbivores and, on the other side an enclosure, which is the "control treatment", fenced and therefore unavailable to any large ungulates. Our main aim was to compute a set of indicators characterizing the ecological changes due to large herbivores pressure on forest ecosystems. We identified 2 categories of ecological indicators: the short-term and the medium-term indicators. Short-term indicators require only two-year of monitoring to correctly quantify herbivore pressure whereas medium-term indicators require at least 4 years of monitoring. The study site is located in Southern Belgium (Wallonia), in mixed beech and oak forests. The predominant vegetation type is the "Luzulo-Fagetum", typically found in acidophileous beech forests. The ungulate species of interest are red deer, roe deer, wild boar and mouflon. In 2006, enclosures and exclosures (4 x 4 m) were installed in 17 sites scattered in two zones with contrasted deer densities to assess indicators efficiency. Between 2006 and 2012, we performed floristic surveys and we recorded the height, density and cover of the understory vegetation of every plot. [less ▲]

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See detailWild boar movement ecology: what do we (don’t) know ?
Morelle, Kevin ULg; Prévot, Céline; Lehaire, François ULg et al

Poster (2013, August)

Although subject of many researches since decades, movement studies have been recently enhanced by the emergence of the movement ecology paradigm (Nathan 2008). Under this paradigm, Nathan et al. (2008 ... [more ▼]

Although subject of many researches since decades, movement studies have been recently enhanced by the emergence of the movement ecology paradigm (Nathan 2008). Under this paradigm, Nathan et al. (2008) proposed to break down movement of animal into four basic mechanistic components: i) internal states (motivation, physiology, why to move ?), ii) motion capacities (how to move ?), iii) navigation capacities and (when and where to move ?) and iv) external factors (physical environment and living organisms – conspecifics or not). Considering these four components of an individual’s movement, we reviewed literature dealing with wild boar (Sus scrofa L.), a species of important ecological and socio-economic concern, and tried to identify the key processes influencing this species’ movement. We conclude this review of the literature by highlighting the gaps in movement ecology of wild boar and suggesting further research directions under the light of the most recent used techniques. [less ▲]

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See detailBioindicators for measurement of red deer pressure on understory vegetation in temperate deciduous forests
Lehaire, François ULg; Morelle, Kevin ULg; Ligot, Gauthier ULg et al

Poster (2013, August)

During the last decades, populations of large herbivores have largely increased. Consequently, their pressure on forest vegetation has been exacerbated, reaching in some cases levels that reduced the ... [more ▼]

During the last decades, populations of large herbivores have largely increased. Consequently, their pressure on forest vegetation has been exacerbated, reaching in some cases levels that reduced the diversity of forest ecosystem services. Assessing the balance between timber production and hunting activities remains a crucial question for forest managers who hence need reliable tools such as ecological indicators. Our aim was to review ecological indicators that characterize the pressure of red deer (Cervus elaphus L.) on understory vegetation in temperate deciduous forest ecosystem. The choice of plants on which the variables are measured is crucial to accurately characterize the deer pressure. This choice must take into account the feeding behavior of red deer, silvicultural objectives, ease of the measurement and the occurrence of these plants within the studied habitat. Generally, it is more appropriate to use common species with a wide ecological amplitude. The choice of indicator plants must take into account plant abundance and palatability, as they both affects considerably the performance of the ecological indicator. The variability of indicators generally increases with the scarcity of the chosen indicator plant. Therefore, choosing abundant plant species appear often the best solution as the resulting have a lower variability and hence enable better to detect changes of deer pressure. At low herbivore pressure, palatable species to offer quick response to the pressure variations. Palatable species are therefore reliable plant indicator especially if these is a good balance between game population and forestry. At excessive herbivore pressure, non-palatable species are preferred. In this case, the variability of ecological indicator is smaller with non palatable plant than with palatable plant. Ecological indicators of deer pressure help to understand the relationships between biodiversity, carrying capacity and deer populations. They are intended to forest managers that would like to monitor red deer pressure in regards to forest management goals and forest sustainability. [less ▲]

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See detailGrowth of Chlorella in vanillin enriched medium
Miazek, Krystian ULg; Goffin, Dorothée ULg; Richel, Aurore ULg

Poster (2013, August)

In this work the effect of different concentration of vanillin on the growth of Chlorella culture was evaluated. Two concentrations of vanillin: 60 mg/L and 300 mg/L in Bold Basal Medium (BBM) were tested ... [more ▼]

In this work the effect of different concentration of vanillin on the growth of Chlorella culture was evaluated. Two concentrations of vanillin: 60 mg/L and 300 mg/L in Bold Basal Medium (BBM) were tested and an inoculum from a two month Chlorella sp. (CCBA) culture was used. Vanillin at concentration of 60 mg/L showed to possess stimulating effect on Chlorella growth during 11 days of cultivation. Stimulation of Chlorella started on 3rd day of growth and was accompanied by 87% decrease of vanillin concentration within first 3 days of cultivation and its complete removal from growth media after 7 days. The acceleration of Chlorella growth in vanillin containing medium was detected due to biomass density, up to 1.2 times bigger than in the control culture, but also by measurement of chlorophyll content. Increased amount of chlorophyll content, up to 1.35 times higher than in control, was found between 4th and 11th day of cultivation. The response of Chlorella towards higher concentration of vanillin (300 mg/L) was different when compared to experiments where only 60 mg/L was used. During first 4 days of cultivation, strong inhibition of Chlorella exposed to 300 mg/L vanillin was observed and vanillin concentration maintained at the same initial concentration. During next days, a recovery effect occurred as biomass density and chlorophyll content gradually increased in comparison to the onset of growth and vanillin concentration decreased to 2 % of its initial value. Biomass density measured in Chlorella culture on 11th day was much higher than at the beginning of cultivation but still by 40% smaller than in control and by 50% smaller than in the culture growing in medium with 60 mg/L of vanillin. Chlorophyll content at the end of cultivation constituted 50% of control value and 35% of chlorophyll culture with 60 mg/L vanillin in medium. [less ▲]

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See detailFine-scale analysis of ungulate-vehicle collisions in Southern Belgium
maron, Julie; Lehaire, François ULg; Morelle, Kevin ULg et al

Poster (2013, August)

Ungulate-vehicle collisions (UVC) are an increasing phenomenon in many European countries. These road accidents are a threat to wildlife populations but also to human safety and generate high economic ... [more ▼]

Ungulate-vehicle collisions (UVC) are an increasing phenomenon in many European countries. These road accidents are a threat to wildlife populations but also to human safety and generate high economic costs. Wallonia, the Southern part of Belgium, is also affected by the UVC problem and offers an interesting study area because of its very dense road network and increasing big game populations. The aim of our study was to determine where and when UVC hotspots occurred along highways in Wallonia, in order to provide recommendations regarding the location and design of mitigation measures. The study site is located in Wallonia (Southern Belgium) in the provinces of Liege and Namur (5,875 km²). Ungulates species present in this area are wild boar, roe deer and red deer. The UVC data were collected by the police and covered the period between 2008 and 2011 (n= 2,704). We analyzed the landscape and road-related variables of sections with high UVC risk in contrast with section of low risk. The landscape and road-related variables related to the location of UVC were highlighted using a generalized linear model (GLM) with simulated pseudo-absences. Concerning traffic accidents, the most involved game species are wild boar (37% of all casualties). That’s why the amount of data on wild boar was higher than for the other species. The results of the spatial and temporal analysis of wild boar-vehicle collisions (WVC) are therefore more accurate than for the other species. Consequently we decided to focused on the wild boar in the present poster. Temporal analysis showed strong variations in the WVC frequency over time, on the daily and seasonal scale. These critical periods correspond to the activity periods of the species (more UVC at night and during autumn and winter). The study also points out a negative correlation between the occurrence of UVC and the traffic volume (R² = 9.79%). This result doesn’t match with the literature but can be explained if we assume that when traffic increase, the road represents a more impassable barrier for animal species. As expected we also noticed a positive correlation between game density and UVC risk. These results indicate clear spatial and temporal clustering of WVC. Identification of hotspots enables us to identify the priority areas where mitigation measures must be considered. For further research, the accuracy of the police data should be improved in order to predict more exactly the risk of UVC. This would also make the mitigation measures more cost-effective. [less ▲]

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See detailA study about the effects of affective valence on a source-monitoring error: cryptomnesia
Beaufort, Aline ULg; Brédart, Serge ULg; Perfect, Timothy J. et al

Poster (2013, August)

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See detailGetting insights on bovine mastitis treatment efficacy based on tissular indicators with an integrated udder health management file: Project LAECEA.
Theron, Léonard ULg; Reding, Edouard; Rao, Anne-Sophie ULg et al

Poster (2013, August)

Mastitis is the most “antibiotic consuming” pathology in dairy medicine. Though antibiotics and antibiograms are known to vets since the early fifties, our practices did not evolved a lot from empiric ... [more ▼]

Mastitis is the most “antibiotic consuming” pathology in dairy medicine. Though antibiotics and antibiograms are known to vets since the early fifties, our practices did not evolved a lot from empiric antibiotic therapy. Indeed, the need for a treatment, the cost and the delay for an antibiogram are most of the time incoherent with a routine practice. Nevertheless, there is a surge for rational use of antibiotics. Our study was based on 1100 mastitis events from 30 Belgian farms collected between January 2011 and June 2012. We chose to compare tissular cure (TC) based on the threshold of 200.000 somatic cells/ml in milk at milk control at least 60 days after the clinical mastitis event. Regarding the mastitis event, severity (according 3 grades: alteration of milk as grade 1, alteration of quarter as grade 2 and alteration of general state as grade 3), quarter, treatments were recorded. We also assessed a chronicity status based on previous somatic cell count (SCC) of the cow. It was considered a new case a cow which at least 15 days before had an SCC <200.000 cells/ml, other were marked as chronic cases. In our distribution, we see a seasonal rise of incidence between January and May. This period would represent twice as many mastitis as the summer period. Overall TC reaches 46% of all mastitis events, which is quite poor. Rear quarters had significantly lower TC (p<0,05%). Grade 3 mastitis had lower TC, 42,6% (p<0.05%) versus 48,9 % for grade 2 and 44,2% for grade 1. Almost 49% of all mastitis was considered as chronic cases, which TC was 33% on average, whereas new cases reached 55,3% TC. Study of treatment was frustrating given the high number of different combinations of treatments. It was underlined that 4th generation cephalosporins (C4G) were the most used in our cohort, followed by aminopenicillin/methicillin association (PENA/PENM) and 1st generation cephalosporins/aminoglycosids (C1G/AG) association. Of these intramammary treatments, 20% of the cases were submitted to a second intramammary drug, mostly C1G or C1G/AG. One third of the cases were treated parenterally with antimicrobials, mostly macrolids, fluoroquinolones and penethacillin. Finally, 10% of mastitis was treated with anti-inflammatory drugs, mostly tolfenamic acid and flunixin-meglumin. Comparing mastitis without use of a secondary intramammary drug, only PENA and C1G/AG reached more than 60% TC. Considering new cases, then C1G/AG, PENA/PENM and Prednisolone containing specialties were above 60% TC. Use of a parenteral injections increased TC only on new cases (+12%), but not on chronic cases. Refining by severity, TC improved with a parenteral on new cases, mainly in grade 1 (+20%). Regarding associated factors, TC was negatively affected by chronicity, parity and lactation stage. Indeed, TC was lower on cases from more than 4 month in milk, third lactation (OR = 2.8 for no cure) compared with previous, and chronic cases (OR=2,6). Seemingly, chronicity was positively associated with parity and season. The 3rd parity cases had higher chances to be chronic ones (OR = 1,7), as well as cases from April to September (OR = 1,6). This evaluation of cure is rather simple and has a good variability which allows several questions about the real match between antimicrobial treatment for mastitis and the udder inflammation. Based on our epidemiological data, we can modify routine management of mastitis, as some cases might not worth the antimicrobial treatment. [less ▲]

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See detailSpatial analysis of bark-stripping damage by red deer in irregular hardwood forest
Lehaire, François ULg; Mercier, Grégoire; Lejeune, Philippe ULg

Poster (2013, August)

Over the past years, the population size of red deer (Cervus elaphus L.) is following an upward trend in Wallonia. This has led to an increased pressure on vegetation as well as to forest damages of ... [more ▼]

Over the past years, the population size of red deer (Cervus elaphus L.) is following an upward trend in Wallonia. This has led to an increased pressure on vegetation as well as to forest damages of greater frequency and intensity. Among these damages, bark-stripping can be especially harmful to the timber quality due to the development of rot. The impact of these populations may thus lead to a reduction in forest productivity and to economic losses. Nevertheless, these damages doesn’t depend only on the density of red deer but also on the environmental characteristics of their habitats. The main objective of this study is to perform a spatial analysis of the occurrence of bark stripping in order to identify the environmental factor that affect the occurrence of the damages in hardwood forests. The bark-stripping damages in coniferous stands are well-known due to the fact that they are relatively frequent. Such information is however unavailable for hardwood stands. The study site (6500 hectares) is located in the Ardennes in Southern Belgium (Wallonia) and is mainly composed of beech stands. We used data of inventory campaigns that were carried out for management purposes. In each sampling unit (total of 321 units), we measured different stand characteristics and, during a second phase, the bark-stripping damages. These latter measurements concerned the presence or absence of bark-stripping on each recorded stem and damage age (presence or not of a healing roll). Only trees with dbh greater than 5 cm were taken into account. All the data were collected in hardwood stands. To perform the spatial analysis, a set of environmental variables, including landscape (distances to the natural or artificial feeding points, to watering-place, to the different types of road and to the forest paths, to refuge areas for deer and to forest edges, etc.) and tree dimension variables, have been collected. The estimated red deer density was also took into account. All these variables have been included in a fixed linear model using stepwise regression. An angular transformation was applied in order to guarantee appropriate conditions of application of the linear regressions. Over one hundred variables were tested but only six of them have a significant impact on the bark-stripping rate. This model explains only 10,2% of the bark-stripping rate. Tree dimension variables explain most of the bark-stripping rate. Thus, forest structure has an important impact on the bark-stripping probability. The roads and the human activities in general can have an impact on the bark-stripping rate. These activities can disturb the red deer feeding periods during the day and lead to important bark-stripping damages. We expected to observe other variables contributing to the model such as red deer density and altitude. The absence of effect of altitude can be explained by the fact that bark-stripping of beech trees occurs mainly in summer. The scale of the study can explain the absence of red deer density. [less ▲]

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See detailThe influence of subjective factors on the evaluation of singing voice accuracy
Larrouy, Pauline ULg; Morsomme, Dominique ULg

Poster (2013, August)

A previous study highlighted the objectivity of music experts when rating the vocal accuracy of sung performances (Larrouy-Maestri, Lévêque, Schön, Giovanni, & Morsomme, 2013). However, in an ecological ... [more ▼]

A previous study highlighted the objectivity of music experts when rating the vocal accuracy of sung performances (Larrouy-Maestri, Lévêque, Schön, Giovanni, & Morsomme, 2013). However, in an ecological context, numerous factors can influence the judges’ assessment of a music performance. This preliminary study aims to examine the effect of the music level of the performers on the evaluation of singing voice accuracy and to explore subjective factors which could influence the assessment. The same sung melody, performed by first and second year students of music conservatory (N = 31), was recorded in the context of their solfeggio examination. The jury, constituted of four music experts, was asked to rate the global pitch accuracy of each student. Two criteria (pitch interval deviation and tonal center deviation) were objectively measured and subjective data about the feeling of the students during the performance (e.g. anxiety level, enjoyment of singing) were collected through questionnaires. The results showed that the criteria used by the jury differed according to the music level of the students. Indeed, while the score of the jury correlated significantly with the vocal accuracy of the second year students, their assessment seemed more subjective concerning the first year students. Interestingly, the score of the jury was significantly correlated with the enjoyment of singing of the first year students and not with the objective measurements (pitch interval deviation and tonal center deviation) anymore. This preliminary work shows the effect of the music level of the performers on the evaluation of singing voice accuracy. Besides the educational implications of these findings, this study describes a promising method for the investigation of subjective factors, which influence the vocal assessment in an ecological context. [less ▲]

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See detailMental Time Travel in First Degree Relatives of Schizophrenia Patients
Boulanger, Marie ULg; Wagener, Aurélie ULg; Duke, Géraldine et al

Poster (2013, July 23)

Introduction. Genetic and environmental factors are important to explain the causation of schizophrenia. The families’ studies can help us to better understand the genetic influence. Indeed, the cognitive ... [more ▼]

Introduction. Genetic and environmental factors are important to explain the causation of schizophrenia. The families’ studies can help us to better understand the genetic influence. Indeed, the cognitive deficits found in the unaffected biological relatives of schizophrenia patients parallel the deficits found in patients. These deficits might be putative endophenotypic markers of schizophrenia, ie. characteristics that mark the presence of a genetic predisposition to a certain disease or disability, in this case, the schizophrenia (Sitskoorn et al., 2004). In this way, previous studies reported that compared to healthy controls (HC), the relatives show cognitive impairments that are similar but milder degree than those of schizophrenia patients (SCh). The deficits affect cognitive functions such as verbal memory, attention and executive function; hence the interests to take into account the endophenotypic markers. To our knowledge, no study has yet assessed the abilities to mental time travel in the relatives despite that this cognitive function is impairment in SCh (Cuervo-Lombard et al., 2007; D’argembeau et al., 2008; Wood and Brewin, 2006). The mental time travel allows individuals both to retrieve past personal autobiographical information and to project into future personal events. Further, it is related to the continuity and the stability of identity, which has also been described as fragmented instable or discontinuous in SCh (Boulanger et al., submitted; deBonis et al., 1995; Nieznanski, 2004). Consequently, the ability to mental time travel could be a relevant endophenotypic marker. Method. 31 SCh, 33 relatives and 31 HC generated both ten past memories and ten future events in response to cue words from TeMA (a French version of AMT). In parallel, they completed cognitive tests, such as the Stroop Color-Word Test, the Trail Making Test, the Digit Span Backward and Forward Test (WAIS-III) and questionnaires which evaluated mood disturbances such as BDI-II and STAI-Y. Finally, participants were asked to respond to a short version of LABEL, which measured identity stability. Results. SCh showed deficits on all measures in comparison to HC. However no significant differences emerged between relatives and HC on measures of time travel. Nevertheless, differences were found for interference score (Stroop-Color-Words Test) and score on part B from TMT, as well as relatives and SCh. Discussion. Despite the fact that the majority of the relatives’ scores on different measures are not significant in comparison to HC and SCh, their results situated between the results of both groups. These results are discussed in the light of previous research. [less ▲]

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See detailRole of non-polyQ regions on the aggregation of polyQ proteins into amyloid fibrils triggered by polyQ expansions
Huynen, Céline ULg

Poster (2013, July 22)

Ten neurodegenerative diseases, referred to as polyglutamine (polyQ) diseases, are associated with the aggregation into amyloid fibrils of ten different proteins containing a polyQ expansion higher than a ... [more ▼]

Ten neurodegenerative diseases, referred to as polyglutamine (polyQ) diseases, are associated with the aggregation into amyloid fibrils of ten different proteins containing a polyQ expansion higher than a pathological threshold comprised between 35 to 45Q (1, 2). A large body of evidence indicate that the polyQ expansion is the critical determinant for the aggregation of these polyQ proteins. The aggregation process of polyQ proteins is, however, still not well understood. To better understand this mechanism at a molecular level, we have characterized model polyQ proteins made of the β-lactamase BlaP from Bacillus licheniformis 749/C and a polyQ tract of 0 to 79Q inserted either at position 197 or position 216 of BlaP. Those chimeras recapitulate the same aggregation behaviours than that of disease-associated polyQ proteins: there is a glutamine threshold for the aggregation into amyloid fibrils and the anticipation phenomenon. Most importantly, the threshold critically depends on the structural integrity of BlaP (3) which would impose some conformational and/or sterical constraints to the polyQ tract. Moreover the position of the polyQ insertion into BlaP modifies the aggregation propensity of BlaP chimeras. The present work aims to further investigate (i) how the protein context affects the different phases of the aggregation phenomenon (i.e. the nucleation and elongation phases) and (ii) the role of the oligomers formed during the early time of the aggregation process. The techniques used are mainly (1) quartz crystal microbalance (QCM) and atomic force microscopy (AFM) to study the elongation step of amyloid fibril formation and (2) dynamic light scattering (DLS) to study the evolution of the different populations formed during the aggregation time course. The results of these experiments indicate that the native conformation of BlaP197(Gln)55 interferes mainly with the nucleation but not with the elongation step of amyloid fibril formation. Moreover, these results demonstrate that the sequences flanking the polyQ tract significantly influence its propensity to elongate amyloid fibrils. Finally, they clearly indicate that the oligomers of BlaP197(Gln)79 observed at the early stage of the aggregation process are on the pathway of amyloid fibril formation, and likely constitute the aggregation nucleus. [less ▲]

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See detailDemographic trends and Streptococcus outbreaks in a synanthropic population of macaques (Macaca fascicularis), Bali (Indonesia)
Brotcorne, Fany ULg; Wandia, I. Nengah; Beudels-Jamar, Roseline et al

Poster (2013, July 22)

The sympatric relationship between humans and primates is a contemporary widespread phenomenon. Several primate species are capable for exploiting human-modified habitats in association with people, but ... [more ▼]

The sympatric relationship between humans and primates is a contemporary widespread phenomenon. Several primate species are capable for exploiting human-modified habitats in association with people, but the most successful species in South-east Asia is probably the long-tailed macaque (M. fascicularis). The low predation pressures in zones of interface and the inclusion of human food in macaques’ diet can lead to local overpopulations. On the other hand, the risk of epidemic disease is simultaneously increased by the high primate density and the proximity with human vectors. Data presented here represent 25 years-population dynamics of a long term commensal-living population of macaques in Ubud Monkey Forest (Indonesia). This population experienced a dramatic growth with an 11% annual increase rate. In June 2012, we counted 615 individuals divided in 5 groups with a very high density of 61 macaques per hectare. However, two Streptococcus outbreaks have also been reported over the same 25 years period, temporarily limiting the steep positive demographic trend of this population. The last epidemic event in July 2012 resulted in a 14% mortality affecting 3 out of 5 groups of the population. Besides the anthropic factors promoting population growth, epidemic diseases play a significant role in shaping the dynamics of this synanthropic population and could have important implications in the future both in terms of local management and local conservation status. [less ▲]

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See detailTracking the growth of Trichoderma reesei during HFBII production - CO2 -HFBII foam
Khalesi, Mohammadreza; Riveros-Galan, David; Deckers, Sylvie et al

Poster (2013, July 21)

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See detailModelling climate change impacts on key tree species used by lion tamarins in the Brazilian Atlantic Forest
Raghunathan, Poornima ULg

Poster (2013, July 21)

We used 3 IPCC climate change scenarios (A1B, A2, B1) in a dynamic vegetation model (CARAIB), to determine the potential future distribution of biomes and 75 species of trees used as food sources or ... [more ▼]

We used 3 IPCC climate change scenarios (A1B, A2, B1) in a dynamic vegetation model (CARAIB), to determine the potential future distribution of biomes and 75 species of trees used as food sources or sleeping sites by endemic primates, the golden lion tamarin (Leontopithecus rosalia) and the golden-headed lion tamarin (L. chrysomelas), in the Brazilian Atlantic Forest (BAF). Habitat conservation is a vital part of strategies to protect endangered species, and this is an approach to understand how key plant species needed for faunal survival might be affected by climate change and what changes to their distribution are likely. CARAIB computes the main physiological reaction of plants, e.g. water absorption or photosynthesis, as a response to temperature, precipitation, or CO2 partial pressure. The model accurately predicted the current distribution of BAF vegetation types and for 66% of the individual tree species with 70% agreement obtained for presence. In the simulation experiments for the future, 72 out of 75 tree species maintained more than 95% of the original distribution and all species showed a range expansion. The results suggested that the trees may benefit from an increase in temperature, if and only if soil water availability is not altered significantly, as was the case with climate simulations that were used. However, the results must be coupled with current and planned land-uses to maximise the usefulness to conservation, as the BAF is subject to many threats. [less ▲]

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See detailThe Anxiety Sensitivity in Children: Developmental Perspective
Stassart, Céline ULg; Etienne, Anne-Marie ULg

Poster (2013, July 19)

Introduction. Anxiety sensitivity (AS) is the fear of anxiety-related sensations due to beliefs that these sensations will lead to physical illness, social embarrassment, loss of control and mental ... [more ▼]

Introduction. Anxiety sensitivity (AS) is the fear of anxiety-related sensations due to beliefs that these sensations will lead to physical illness, social embarrassment, loss of control and mental incapacitation. AS plays a central role in the etiology and maintaining of fear and anxiety. This work examines the gender and age effect on the AS scores. Method. Two hundred children aged 9 to 13 years completed the Childhood Anxiety Sensitivity Index (CASI). Results. Hierarchical multiple regression analyses showed that the sex and the interaction Sex*Age explain significantly the CASI scores. The interaction indicated that girls have higher AS than boys at the age of 9, 10 and 11 but not to 12 and 13 years. Discussion. Consistent with several studies, girls have higher CASI scores than boys. However, this difference disappears at the entrance of adolescence. This developmental observation is important in a prevention perspective of AS. [less ▲]

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See detailDevelopment of Purkinje cells in the ovine brain
Salouci, Moustafa ULg; Engelen, Virginie; Gyan, Mathilda et al

Poster (2013, July 18)

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were ... [more ▼]

Purkinje cells are involved in many vital functions within the body. Twenty ovine fetuses ranging from 2 to 5 months of gestation, two lambs in the first week after birth and three adult sheep were studied. Sections of the cerebellum were stained with haematoxylin and eosin, cresyl violet and Klu¨ver–Barrera. This study indicates that Purkinje cells began to appear after the 15th week of gestation. There were varying degrees of development of Purkinje cells in different zones of the cerebellum. Our findings in sheep fetuses suggest that the maturation of Purkinje cells starts in the caudal regions of the cerebellum and that the process begins in the vermis before it does in the cerebellar hemispheres. The alignment of Purkinje cells was found to be very regular in the caudal regions of the cerebellum. A partial absence of Purkinje cells in the rostral regions of the cerebellum was observed in both sheep fetuses and adult sheep. In the first post-natal week, some ectopic Purkinje cells were found in the white matter of the cerebellum. [less ▲]

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See detailGovernance in Japan and Belgium: Building on Experiments with Technology Assessment and TA-like Activities
Van Oudheusden, Michiel ULg; Yoshizawa, Go

Poster (2013, July 15)

This poster presents a bilateral Belgian-Japanese research study that probes the direction and quality of science and technology governance within the context of new knowledge production and responsible ... [more ▼]

This poster presents a bilateral Belgian-Japanese research study that probes the direction and quality of science and technology governance within the context of new knowledge production and responsible innovation. Drawing on recent experiences with technology assessment (TA) and TA-like activities in the regions of Flanders and Wallonia (Belgium) and the country of Japan, it asks how future-oriented science and technology governance processes are locally enacted and how they compare. Four research objectives are discerned: 1. To describe how TA concepts and practices, as well as related TA activities, have emerged in Japan, Wallonia, and Flanders since the 1960s-70s, and in what particular forms. 2. To describe how TA has evolved with, sustained, and/or countered, science, technology, and innovation policies on the regional, national, and international level. 3. To compare and contrast how TA is, or is not, institutionalized in the countries and regions, notably by taking into account initiatives to initiate or abolish parliamentary TA forms. 4. To situate the processes that are discerned through empirical analysis within a broader theory of, and movement towards anticipatory governance, and to assess the potential of TA of enhancing novel governance forms. The poster situates science, technology, and innovation policies in Japan and Belgium within the global shift towards a knowledge-based economy and the emergence of new science policy regimes, such as “strategic science” (Rip, 2002). Building on several TA case examples, it explains how the need for TA in science and technology emerges and is developed within distinctly different innovation contexts. The poster describes the project’s methodologies, working plan, and expected results, and provides suggestions for rendering comparative TA analysis useful to science policymakers and innovation actors, as well as to science and technology studies scholars. [less ▲]

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See detailTowards the first transmission spectrum of a gas giant transiting an M-dwarf
Delrez, Laetitia ULg; Gillon, Michaël ULg; Lendl, M. et al

Poster (2013, July 15)

At the forefront of comparative exoplanetology, the atmospheric characterization of transiting exoplanets is revealing the intimate nature of these new worlds. In this exciting context, we are currently ... [more ▼]

At the forefront of comparative exoplanetology, the atmospheric characterization of transiting exoplanets is revealing the intimate nature of these new worlds. In this exciting context, we are currently conducting a VLT observing campaign on a rare exoplanet specimen, WASP-80b, a gas giant in close orbit around a bright nearby M-dwarf. Even if this planet belongs to the hot-Jupiter population, it is actually more ‘warm’ than ‘hot’ with an estimated equilibrium temperature of only 800K. We present here some preliminary results of this program which consists in monitoring four transits of WASP-80b with the FORS2 instrument in multi-object spectroscopic mode in ESO phase 91. Through this approach, our goal is to precisely measure the transmission spectrum of the planet between 740 and 1070 nm in order to constrain the thermal structure and scacering properties of the planetary atmosphere. Furthermore, we will use the water features located around 950 nm to constrain the water mixing ratio in the atmosphere of this peculiar hot Jupiter. [less ▲]

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See detailConcomitant inhibition of class I HDAC and COX-2 exerts a antitumor effect in a human pancreatic cancer model
Gonzalez, Arnaud ULg; Peixoto, Paul ULg; Turtoi, Andrei ULg et al

Poster (2013, July 11)

- Introduction : Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer death in developed countries. Early-stage pancreatic cancer is usually clinically silent, and disease only ... [more ▼]

- Introduction : Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer death in developed countries. Early-stage pancreatic cancer is usually clinically silent, and disease only becomes apparent after the tumor invades surrounding tissues or metastatises to distant organs. Moreover, the current chemotherapeutic treatments have no or few effects on this type of cancer, increasing only slightly the median survival of the patients. The survival rate at 5-years is only 3%. There is a need to develop new effective therapies for PDAC patients together with a robust and fast in vivo model allowing drug screening. In this study, We tested whether the combined inhibition of cyclooxygenase-2 (COX-2) and class I histone deacetylase (HDAC) may result in a better control of PDAC. We improved the formation of pancreatic tumor on Chorioallantoic membrane (CAM), an alternative to murine model. - Methods : The impact of the concomitant HDAC and COX-2 inhibition on cell growth, apoptosis and cell cycle was assessed in vitro on human pancreas BxPC-3 cells treated with chemical inhibitors (SAHA, MS-275 and celecoxib) or HDAC1/3/7 siRNA. To test the potential antitumoral activity of this combination in vivo, we improved, characterized and used model of pancreas tumors growing on chick chorioallantoic membrane. - Results : The inhibition of HDAC1/3 by SiRNA or MS-275 treatment reduced significantly the growth of BxPC-3 cells in vitro. Furthermore, we showed by QPCR and immunoblotting that both HDAC1 and HDAC3 inhibition induced the expression of COX-2 at least via the NF-kB pathway. Based on this observation, we decided to test the effect of MS-275 combined with celecoxib a COX-2 inhibitor. This combination was more effective then either drug used alone to reduce the growth of BxPC-3 cells. By FACS analysis we showed that MS-275/celecoxib combination decreased significantly the proportion of cells in S phase and increased significantly and drastically the proportion in G0/G1 at 24, 48 and 72h. By immunobloting this GO/G1 arrest was confirmed by accumulation of cell cycle repressors (P21, P27) and disappearance of hyper phosphorylated form of RB protein. Following a procedure development, we produced on CAM 60 mm3 functionally vascularized tumors mimicking human pancreatic tumors on CAM model. The clinical relevance of this model is supported by the CK7+/CK19+/CK20-/CEA+/Ki67+/CD56- immunolabeling. Recently we have discovered several novel biomarkers of human PDAC: MYOF, TGFBI, LTBP2. These antigens were expressed in tumors grown on CAM, reaffirming its clinical relevance. The concept of the co-treatment by MS-275 and celecoxib was validated using this model. We showed that celecoxib alone did not significantly reduce tumor growth. MS-275 alone decreased tumor growth by 50% and combination of celecoxib and MS-275 stalled entirely the tumor growth. - Conclusions : Our data demonstrate a significant synergic anti-tumoral action of HDAC and COX-2 inhibitors, which set a basis for the development of potentially effective new combinatory therapies for PDAC patients. [less ▲]

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See detailGenetic analysis of longitudinal measurements of feed intake in Piétrain sire lines
Dufrasne, Marie ULg; Jaspart, Véronique; Wavreille, José et al

Poster (2013, July 10)

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See detailMetallo-supramolecular micellar gels: a structural study
Mugemana, Clément; Joset, Arnaud ULg; Guillet, Pierre et al

Poster (2013, July 10)

Detailed reference viewed: 9 (2 ULg)
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See detailFirst spike latency sensitivity of spiking neuron models
Trotta, Laura ULg; Franci, Alessio ULg; Sepulchre, Rodolphe ULg

Poster (2013, July 08)

First spike latency is the long-lasting period preceding the first spike of a neuron submitted to a super-threshold stimulus. It has been suggested that this latency could code for stimulus recognition in ... [more ▼]

First spike latency is the long-lasting period preceding the first spike of a neuron submitted to a super-threshold stimulus. It has been suggested that this latency could code for stimulus recognition in several sensory systems. To encode information reliably, first spike latency should be sensitive to sensory inputs but robust to external perturbations. This paper studies the robustness of the first spike latency in spiking neuron models. We show the interplay between bistability, first spike latency and a type of neuronal excitability called regenerative excitability. [less ▲]

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See detailBiomimetic polymers
Clement, Benoît ULg; Vanslambrouck, Stéphanie ULg; Koole, Leo H. et al

Poster (2013, July 08)

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See detailThe effects of flowering on root development in Arabidopsis
Mathieu, Laura ULg; Lobet, Guillaume ULg; Périlleux, Claire ULg

Poster (2013, July 05)

Flowering in Arabidopsis thaliana is controlled by photoperiod. In long days, the mobile proteins FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF) are produced in the leaves and transported in the ... [more ▼]

Flowering in Arabidopsis thaliana is controlled by photoperiod. In long days, the mobile proteins FLOWERING LOCUS T (FT) and TWIN SISTER OF FT (TSF) are produced in the leaves and transported in the phloem toward the shoot apical meristem (SAM). In the SAM, FT and TSF interact with the transcription factor FD to activate the expression of genes responsible for floral meristem identity [1]. Since the formation of flowers and fruits is a costly and critical stage for the plant, it is expected to be supported by a well developed root system to insure the increase in plant’s needs. As a first step to understand the effects of flowering on root growth, we compared root development in long days, promoting flowering, and in short days (16h and 8h days, respectively). [less ▲]

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See detailEliciteurs dérivés de rhamnolipides : synthèses, modélisations et activités biologiques
Mayon, Patrick; Ait Barka, Essaid; Baillieul, Fabienne et al

Poster (2013, July 04)

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See detailIMPACT OF COVER CROP AND CROP RESIDUE MANAGEMENT ON CROP PRODUCTION
Hiel, Marie-Pierre ULg; Bodson, Bernard ULg

Poster (2013, July 03)

The aim of this project is to study the crop development in relation to soil tillage and organic residue management and to understand its impact on crop production but also on the dynamics of weed ... [more ▼]

The aim of this project is to study the crop development in relation to soil tillage and organic residue management and to understand its impact on crop production but also on the dynamics of weed populations and intensity of fungal diseases occurring on the crops. Various cropping systems with different tillage methods dedicated to bury the residues from the previous crop and/or from the intercropping and to prepare the implantation of following crop are studied. [less ▲]

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See detailImpact d'un fractionnement soustractif sur la relation structure-fonction de la gomme de caroube
Gillet, Sébastien ULg; Blecker, Christophe ULg; Aguedo, Mario ULg et al

Poster (2013, July 02)

Le choix d'une température de fractionnement de la gomme de caroube va conditionner les caractéristiques des fractions obtenues et par conséquent les potentialité d'applications de ces dernières.

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See detailContribution à la détermination du profil en COVs des effluents gazeux de séchage industriel de levain
Eloundou Mballa, Pierre Patrick ULg; Fauconnier, Marie-Laure ULg

Poster (2013, July 02)

During the industrial drying of sourdough, there is an evaporation of a part of the volatile compounds and those generated during the process. These compounds are evacuated with waste and lost for the ... [more ▼]

During the industrial drying of sourdough, there is an evaporation of a part of the volatile compounds and those generated during the process. These compounds are evacuated with waste and lost for the industry. A complete characterization of the aromatic profile would open ways towards a co-valuation of some of these COVS. [less ▲]

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See detailSPECULOOS: Search for habitable Planets EClipsing ULtra-cOOl Stars
Gillon, Michaël ULg; Jehin, Emmanuel ULg; Delrez, Laetitia ULg et al

Poster (2013, July 01)

The 1000 nearest ultra-cool stars (spectral type M6 and latter) represent a unique opportunity for the search for life outside our solar system. Due to their small luminosity, their habitable zone is 30 ... [more ▼]

The 1000 nearest ultra-cool stars (spectral type M6 and latter) represent a unique opportunity for the search for life outside our solar system. Due to their small luminosity, their habitable zone is 30-100 times closer than for the Sun, the corresponding orbital periods ranging from one to a few days. Thanks to this proximity, the transits of a habitable planet are much more probable and frequent than for an Earth-Sun analog, while their tiny size (about 1 Jupiter radius) leads to transits deep enough for a ground-based detection, even for sub-Earth size planets. Furthermore, a habitable planet transiting one of these nearby ultra-cool stars would be amenable for a thorough atmospheric characterization, including the detection of possible biosignatures, notably with the near-to-come JWST. Motivated by these reasons, we have set up the concept of a ground-based survey optimized for detecting planets of Earth-size and below transiting the nearest Southern ultra-cool stars. We present here this concept and its status. [less ▲]

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See detailThe influence of calcium carbonate-wheat flour mix addition on sunflower halva stability and texture
Muresan, Vlad; Racolta, Emil; Muste, Sevastita et al

Poster (2013, July 01)

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See detailInfluence of emotional content of items on cryptomnesia
Beaufort, Aline ULg; Brédart, Serge ULg; Perfect, Timothy J. et al

Poster (2013, July)

Although the link between emotion and memory has been demonstrated for long, only one study has examined the impact of emotion on inadvertent plagiarism (cryptomnesia) up to now (Gingerich & Dodson, 2012 ... [more ▼]

Although the link between emotion and memory has been demonstrated for long, only one study has examined the impact of emotion on inadvertent plagiarism (cryptomnesia) up to now (Gingerich & Dodson, 2012). The Gingerich and Dodson‘s experiment examined the impact of mood on unintentional plagiarism. The present study examined the effect of emotional content of items on the occurrence of unintentional plagiarism using the Brown and Murphy paradigm (1989). In a first stage, same-sex dyads (96 participants, 48 men, mean age=21.5 years) were asked to generate alternately words corresponding to an emotional category. Three categories were proposed to our participants: positive, neutral and negative. Participants returned after a one week-delay and were instructed (1) to recall the items generated by themselves one week earlier (RO task), (2) to generate four new items for each category (GN task), and (3) to assign a confidence rating. In the RO task, almost 17% of responses were plagiarisms and the percentage almost reached 9% in the GN task. In the RO task, plagiarism was significantly higher for positive than neutral items. In addition, positive and negative items were better recalled than neutral one. These results demonstrate an impact of the emotional content on inadvertent plagiarism. [less ▲]

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See detailReferee education in Wallonia - The case of the team sports with interpenetration
Van Hoye, Martin ULg; Mottard, Baptiste; Cloes, Marc ULg

Poster (2013, July)

The referees are responsible for the proper progress of sports competitions. However, it is clear that very few studies are devoted to them, particularly with regard to their training. The aim of this ... [more ▼]

The referees are responsible for the proper progress of sports competitions. However, it is clear that very few studies are devoted to them, particularly with regard to their training. The aim of this study was to analyze the Wallonian situation in five federations: basketball, football, handball, hockey and rugby. We conducted semi-structured interviews with three officials of their refereeing commission. After the analysis of the data, subjects gave a feedback on the synthesis of the interviews (Friedberg, 1994). The SONAL software (v.1.6.11; Alber, 2010) allowed us to process the data. According to our subjects, no federation is genuinely interested by referees, as they prefer to focus more on other aspects ("sport for all", "promotion of their sport", "notoriety of the national team" …). The referee courses are purely theoretical and concluded with an examination (15/15). Then, supervisors follow the referees on the field but the lack of human resources is a real problem (15/15). Only the basketball federation offers learning levels, allowing a gradual advancement. The career-long learning is based on supervisions but also on seminars. The field's experience is the most important factor (15/15) to improve (Pizerra and Laborde, 2011). We noticed also that the football federation implemented refereeing training centers while the basketball federation has established a school of refereeing. In addition, the rugby referee educators must have a certificate of the international refereeing commission while any special skills are requested in the other federations. Except in hockey, all courses are free. In the five federations, the structures are extremely different. An identical framework with different invariable levels, as in coach education (Theunissen, 2007), could facilitate the implementation of interdisciplinary training. [less ▲]

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See detailEvaluating the psychological impact of practice dispatch-assisted cardiopulmonary instructions using the ALERT protocol: preliminary results in Liege dispatching centre.
Hirtz, Elodie; Ghuysen, Alexandre ULg; STIPULANTE, Samuel ULg et al

Poster (2013, July)

ABSTRACT Background. The ALERT algorithm, an effective compression-only phone cardiopulmonary resuscitation (CPR) protocol has the potential to help bystanders initiate CPR. This study evaluates the ... [more ▼]

ABSTRACT Background. The ALERT algorithm, an effective compression-only phone cardiopulmonary resuscitation (CPR) protocol has the potential to help bystanders initiate CPR. This study evaluates the psychological impact of the CPR’s practice on untrained persons (UP). Methods. This is a quasi experimental longitudinal study (n = 153). We used: demographics data, CPR’ emotional characteristics; the Peritraumatic Dissociative Experiences Questionnaire ; the Way of Coping Check List and the Impact of Event Scale. Findings. Two psychological profiles: UP at high risk to develop a post traumatic disorder (higher average scores ; high emotional distress during the CPR) versus UP at low risk. Discussion. These preliminary results highlight the importance of identifying the psychological profile of the UP. For a CPR, UP at high risk should be treated differently: first, take the time to reduce emotional distress and then only talk about the CPR. This step could reduce the risk for PTSD [less ▲]

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See detailStudy and conservation of Antarctic cyanobacterial diversity in the BCCM/ULC collection
Wilmotte, Annick ULg; Renard, Marine; Ould Amer, Yasmine et al

Poster (2013, July)

The BCCM/ULC public collection presently includes 72 Antarctic cyanobacterial strains and its catalogue is available on http://bccm.belspo.be/db/ulc_search_form.php. An ISO9001 certificate was obtained ... [more ▼]

The BCCM/ULC public collection presently includes 72 Antarctic cyanobacterial strains and its catalogue is available on http://bccm.belspo.be/db/ulc_search_form.php. An ISO9001 certificate was obtained for the public deposition and distribution of strains. The purpose of this collection is to gather a representative sample of Antarctic cyanobacterial diversity from different biotopes (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the diversity, evolution, physiology, and genomic make-up. This is particularly important in view of the emerging use of metagenomic approaches on environmental samples, where the comparison with well-defined strain genome sequences can be very useful. [less ▲]

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See detailRecursive PCA of e-nose data for anaerobic digestion reactor state monitoring
Adam, Gilles ULg; Lemaigre, Sébastien; Romain, Anne-Claude ULg et al

Poster (2013, July)

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See detailDo fluid intelligence and executive functioning protect from age-related decline in cognitive control?
Manard, Marine ULg; Lorant, Caroline ULg; Collette, Fabienne ULg

Poster (2013, July)

Age-related studies on cognitive control suggest a decline in proactive control whereas reactive control remains intact (Braver, Gray, & Burgess, 2007). This study was designed to investigate the ... [more ▼]

Age-related studies on cognitive control suggest a decline in proactive control whereas reactive control remains intact (Braver, Gray, & Burgess, 2007). This study was designed to investigate the potential influence of fluid intelligence (Raven’s Advanced Progressive Matrices) and general executive functioning (Executive composite score from executive assessment) on cognitive control abilities during normal aging. Cognitive control was assessed using a computerized version of the Stroop paradigm in which proactive and reactive control processes were pseudo randomly engaged by varying the amount of interference across the task. Thirteen young and 27 older adults were included in this study. In the whole sample, results confirmed a significant age-related decline in proactive control. However, when older adults were divided in high- and low fluid intelligence subgroups, only older adults with low fluid intelligence showed a significant decline in proactive control compared to younger adults and this difference disappeared for older adults with high fluid intelligence level. When older adults were distinguished according to high and low executive capacities, the analysis revealed that, compared to the young ones, older adults with low executive functioning showed a general cognitive control decline (proactive and reactive processes). However, differences disappeared when young adults were compared to older adults with a high executive functioning. Accuracy results suggested that older adults have a slower but spared access to goal-representations that are necessary to correctly manage interference. So, this study confirmed the previous results obtained by Braver et al. (2007), but also suggest an influence of fluid intelligence and executive resources that seems important to take into account to investigate age-related decline in reactive and proactive cognitive control processes. [less ▲]

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See detailPerformance of the IDS-iSYS 1,25-dihydroxy vitamin D assay
GADISSEUR, Romy ULg; LUKAS, Pierre ULg; CARLISI, Ignazia ULg et al

Poster (2013, July)

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See detailNew format of the quality of life systemic inventory for children (QLSI-C): preliminary results
Toucheque, Malorie ULg; Tilkin, Caroline; Etienne, Anne-Marie ULg

Poster (2013, July)

Background: This study assesses the test-retest reliability of the QLSI-C iPad version. Methods: Sample consisted of 40 children aged 8 to 12 years which completed QLSI-C twice over a two-week delay ... [more ▼]

Background: This study assesses the test-retest reliability of the QLSI-C iPad version. Methods: Sample consisted of 40 children aged 8 to 12 years which completed QLSI-C twice over a two-week delay. Participants were divided into 3 groups: 13 participants completed iPad (T0) – paper (T1) version, 13 others filled paper (T0) – iPad (T1) and 14 answered iPad (T0) – iPad (T1). Findings: Analysis of variance indicates that group effect (F(2,37) = 1,27, p = 0,29) and time effect (F(2,37) = 0,04, p= 0,84) is not statistically significant nor the interaction effect (F(2,37) = 0,08 p = 0,92). Correlations for the 5 global scores between iPad – iPad version is from .63 to .91 and between the 2 different formats is from .40 to .87. Discussion: QLSI-C test-retest stability and paper – iPad version equivalence are good. This new format is more attractive for children, decreases time for administration and makes easier the encoding. [less ▲]

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See detailAge estimation from faces in Alzheimer disease
Moyse, Evelyne ULg; Brédart, Serge ULg; Salmon, Eric ULg et al

Poster (2013, July)

Although studies on age estimation showed that the performance of estimation is fairly accurate, this performance can be influenced by group biases such as the own-age bias (George & Hole, 1995). Moreover ... [more ▼]

Although studies on age estimation showed that the performance of estimation is fairly accurate, this performance can be influenced by group biases such as the own-age bias (George & Hole, 1995). Moreover this bias occurs both in young and older adults (Moyse & Brédart, 2012). Because difficulties in face processing have been reported in Alzheimer disease (Della Sala et al., 1995), the aim of this study was to examine the performance of age estimation from faces in patients with Alzheimer disease (mild to moderate) compared with normal aging persons. Moreover to test the preservation of the occurrence of an own-age bias, stimuli belonging to different age groups (young, middle age and older adults) were used. We observed a main effect of Group indicating that patients were less accurate than control whatever the age of faces. In addition a main effect of Age of faces was obtained; the percentage of accuracy was better for older faces than for the two other age groups of faces. Consequently although patients’ performance in age estimation of faces is impaired, an own-age bias was still present. These results have two main interests: a clinical interest (expanding the diagnostic criteria of the Alzheimer disease) and a forensic interest (assessing the credibility of eyewitness testimony in older adults with a possible Alzheimer disease). [less ▲]

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See detailBiodiversity studies in Open-Top Chambers in continental Antarctica
Mano, Marie-José ULg; Namsaraev, Zorigto; Obbels, Dagmar et al

Poster (2013, July)

Open Top Chambers are passive warming systems used to experimentally assess the effect of climate change on terrestrial ecosystems, and they were used in several Antarctic regions to study the response of ... [more ▼]

Open Top Chambers are passive warming systems used to experimentally assess the effect of climate change on terrestrial ecosystems, and they were used in several Antarctic regions to study the response of biotic communities. In the BELSPO BELDIVA project, OTCs were used in continental Eastern Antarctica, where environmental conditions are very extreme. In January 2010, 8 Open-Top Chambers (OTC) were installed in four ice-free regions of the Sör Rondane Mountains, namely on the Utsteinen ridge, the Tanngarden granite outcrop, the Teltet nunatak and the fourth nunatak of the Pingvinane range. [less ▲]

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See detailBioregionalisation and biodiversity of Cyanobacterial communities in East- Antarctic lakes
De Carvalho Maalouf, Pedro ULg; Stelmach Pessi, Igor ULg; Verleyen, Elie et al

Poster (2013, July)

On the Antarctic continent, ice-free regions represent only 0.3% of the total surface area. In these regions, cyanobacteria are one of the dominant phototrophs and primary producers, forming cohesive ... [more ▼]

On the Antarctic continent, ice-free regions represent only 0.3% of the total surface area. In these regions, cyanobacteria are one of the dominant phototrophs and primary producers, forming cohesive benthic mats in lakes and other aquatic habitats. Using a recent compilation of published biodiversity data from Antarctica and the sub-Antarctic islands in combination with physical factors and expert-defined bioregions, Terauds et al. (2012) identified 15 biologically distinct ice-free Antarctic Conservation Biogeographic Regions (ACBRs). However, the degree and nature of bioregionalisation in Antarctic cyanobacterial communities is still largely unknown. In this study, cyanobacterial diversity was analysed using Denaturating Gradient Gel Electrophoresis (DGGE) on a partial 16S rRNA sequence (ca 400 bp) in 50 microbial mats originating from five of the seven ACBRs identified in East Antarctica. Comparison of the sequences with those available in GenBank was carried out to assess the geographical distribution of the taxa on both local and global scales. A total of 35 Operational Taxonomic Units (OTUs, group of sequences with more than 98.5% sequence similarity) was obtained for all samples. Moreover, the taxonomic turnover between the five ACBRs was relatively low, as 69.5% of the OTUs were present in 2 or 3 bioregions. Consistent with previous findings, a large proportion (37%) of the OTUs was restricted to the “cold biosphere”, and 17% appeared potentially endemic to the Antarctic continent. These results suggest that the patterns of bioregionalisation in East Antarctic cyanobacteria are different than the ACBR scheme. Therefore, it would be useful to study the microbial bioregionalisation in more detail and using less conserved molecular taxonomic markers than the 16S rRNA gene, such as the ITS region. Moreover, since DGGE only provides information on the dominant taxa in microbial assemblages, a high-throughput screening is currently being performed. [less ▲]

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See detailJob satisfaction and self-efficacy to manage dementia in home caregivers: Effects of a three-day intervention
Marquet, Manon ULg; Missotten, Pierre ULg; Charlot, Valentine ULg et al

Poster (2013, July)

There is a growing need for interventions designed to help professionals who care for people with dementia. Nevertheless, most existing programs are developed for professionals working in institutional ... [more ▼]

There is a growing need for interventions designed to help professionals who care for people with dementia. Nevertheless, most existing programs are developed for professionals working in institutional settings. Thus, the objectives of our study are to evaluate the effectiveness of a three-day program aimed at training home help services (n=19). This intervention is targeted to enhance knowledge about dementia and help professionals to develop problem-solving strategies applicable to their everyday practice. Statistical analyses were based on self-report questionnaires administered before and after the intervention. Results indicate that participants find focused-problem coping to be more helpful after training and have better knowledge about dementia. Moreover, the program produces enhancement of job satisfaction and lower ageism scores. These findings are promising and suggest that a brief program can be effective to help caregivers of home-based services dealing with dementia problems. [less ▲]

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See detailTiO2 templated films used as photoelectrode for solid-state DSSC applications: study of the pore filling by Rutherford Backscattering Spectroscopy
Dewalque, Jennifer ULg; Colson, Pierre ULg; Thalluri, Venkata Visveswara Gopala Kris ULg et al

Poster (2013, July)

Mesoporous anatase thin films are very promising materials to act as electrode in dye-sensitized solar cells. Randomly oriented nanocrystalline TiO2 particles are usually used to prepare photoelectrodes ... [more ▼]

Mesoporous anatase thin films are very promising materials to act as electrode in dye-sensitized solar cells. Randomly oriented nanocrystalline TiO2 particles are usually used to prepare photoelectrodes with a thickness of 10-15 µm. However, in solid-state DSSCs, TiO2 films thickness is limited to few µm allowing the adsorption of only a low quantity of photoactive dye and thus leading to a poor light harvesting and low conversion efficiencies. In order to overcome this limitation, templated-assisted dip-coating techniques are used to obtain thin films with high surface area. Moreover, templating is expected to improve the pore accessibility what would promote the solid electrolyte penetration inside the porous network, making possible efficient charge transfers. In this study, films prepared from different structuring agents are discussed in terms of microstructure properties (porosity, crystallinity) and impact on the dye loading and solid electrolyte filling. As-obtained templated films have been compared to nanocrystalline films prepared by doctor blade or screen printing as reference. Different techniques such as transmission electron microscopy (TEM), atmospheric poroellipsometry (AEP) and UV-visible absorption spectroscopy (UV-vis.) have been used to describe the microstructural features of the films. Solid electrolyte infiltration has been extensively investigated by Rutherford Backscattering Spectroscopy (RBS). Finally, templated films were evaluated as photoelectrode in solid-state DSSCs and compared to nanoparticles layers. [less ▲]

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See detailCHANGING THE ELECTRONIC DENSITY OF THE DISTAL PHENYL RING OF 4-ARYL-1,2,3,6-TETRAHYDROPYRIDINE DERIVATIVES TO INFLUENCE THE AFFINITY FOR 5-HT1A RECEPTORS
Liégeois, Jean-François ULg; Lespagnard, Marc; Meneses-Salas, Elsa et al

Poster (2013, July)

4-Phenyl-1,2,3,6-tetrahydropyridine(THP) moiety was shown to be favourable compared to the classical 4-phenyl-piperazine in terms of affinity for 5-HT1A receptors in a series of 4-arylpiperazine-ethyl ... [more ▼]

4-Phenyl-1,2,3,6-tetrahydropyridine(THP) moiety was shown to be favourable compared to the classical 4-phenyl-piperazine in terms of affinity for 5-HT1A receptors in a series of 4-arylpiperazine-ethyl carboxamides. The almost planar orientation found in the 4-phenyl-1,2,3,6-THP compounds appeared to be an important spatial requirement for an optimal interaction with the 5-HT1A receptor in this series. This orientation tends to stabilize the ligand binding by an edge-to-face CH-Π interaction between the phenyl ring of the 4-phenyl-1,2,3,6-THP compounds and the side chain of the Phe 6.52 residue in the binding pocket .In the present study, the electronic distribution of the distal benzene ring is modified by introducing substituents with different electronic and/or physicochemical characteristics to explore the impact on this interaction on the affinity for 5-HT1A receptors. These substituents were placed either in 4 or 3 and 5 position of the distal ring in order to avoid a sterical constraint if present in 2 or 6 position. In a quinoxalinamide series, the 3,5-dimethyl substituted analogue has the highest affinity for 5-HT1A receptors as expected. A reinforced interaction with the Phe 6.52 residue might explain this affinity but increased basicity and lipophilicity might also have a role. [less ▲]

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See detailDevelopment and validation of the working memory self-assessment scale
Fresson, Megan ULg; DEMOULIN, Valentine ULg; HIERNAUX, Anne ULg et al

Poster (2013, July)

Aim. Because working memory is involved in many daily life activities, its ecological evaluation is a key dimension of the neuropsychological assessment of people with cognitive impairments. The aim of ... [more ▼]

Aim. Because working memory is involved in many daily life activities, its ecological evaluation is a key dimension of the neuropsychological assessment of people with cognitive impairments. The aim of this study was to develop and validate a 30-items self-assessment scale of working memory, the WMSS (Working Memory Self-Assessment Scale). Method. The WMSS and a comprehensive assessment battery of working memory and executive functions were administered to sixty French-speaking individuals (20 young, 20 old, 20 old-old). Results. The internal validity of the scale was strong as estimated by the Cronbach’s alpha coefficient (α = .93). Concerning the external validity, several correlations were obtained between the WMSS and the cognitive composite scores. Unlike old subjects, the lower cognitive results young and old-old subjects had, the more working memory complaints they expressed. Discussion. The WMSS shows a satisfactory internal as well as external validity since young and old-old subjects who reported more difficulties (WMSS) are those who obtained lower cognitive results. The somewhat surprising relationship between the WMSS and the cognitive tasks in the old group can be explained by an increased perception of cognitive changes and a more complex life style in old subjects with better cognitive abilities. [less ▲]

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See detailMorphometric analyses of the normal suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir ULg; Antoine, Nadine ULg; Piret, Joëlle ULg et al

Poster (2013, July)

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in ... [more ▼]

The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in sound horses. Right limbs from 11 horses were collected. Samples from 6 levels of the SL were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues allowing the use of an image analysis programme. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the SL. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the SL of sound horses. [less ▲]

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See detailThe diversity and tolerance to osmotic stress of East Antarctic filamentous Cyanobacteria
Obbels, Dagmar; Verleyen, Elie; Tytgat, Bjorn et al

Poster (2013, July)

Filamentous cyanobacteria are keystone species in Antarctic lake ecosystems; they are the basis of the simple foodwebs, play a crucial role in biogeochemical cycling and form the structure of benthic ... [more ▼]

Filamentous cyanobacteria are keystone species in Antarctic lake ecosystems; they are the basis of the simple foodwebs, play a crucial role in biogeochemical cycling and form the structure of benthic microbial mats which act as habitats for other prokaryotic and (micro-eukaryotic biota. Despite this, little is known about their diversity, adaptation and survival strategies in the extreme Antarctic conditions. We studied the uncultivated prokaryotic diversity using a 454 metagenomic analysis at the 16S rRNA level (V1-V3 region) in Continental Antarctic lakes situated along a conductivity gradient (0.014-142.02 mS/cm). The quality and length of the amplicons was analyzed with a custom-made Mothur pipeline and the resulting sequences were mapped against the Greengenes database, which includes CyanoDB. Almost 27% of the sequences could be assigned to the phylum of the cyanobacteria. The most abundant cyanobacteria in the dataset belonged to the genera Microcoleus, Leptolyngbya, Pseudanabaena, Nodularia and Phormidum. Some 16S rRNA types (at the 97% similarity level), such as sequences related to Leptolynbya antarctica, were present in both freshwater and hypersaline lakes. In order to further investigate this distribution, we isolated filaments of Leptolyngbya from seven lakes with conductivities ranging between 26.8 mS/cm and 0.038 mS/cm. The complete 16S rRNA and ITS genes of the isolates were subsequently sequenced. We found several 16S types related to different lineages of filamentous cyanobacteria in the seven lakes that were supported by ITS data. Two 16S types, belonging to a Leptolyngbya antarctica and Leptolyngbya sp., were each present in two different freshwater lakes. Two different 16S types, both belonging to Leptolynbya antarctica were present in a freshwater and hypersaline lake, which indicates a high ‘intraspecific’ molecular diversity. In order to better understand the adaptation and/or wide tolerance to osmotic stress, we are currently performing ecophysiological experiments with these isolates aimed at assessing the potential local adaptation of these strains to conductivity and desiccation. [less ▲]

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See detailEffect of bio-sourced compounds on activation of immune cells in the view of to improve immunotherapy of malignant pleural mesothelioma
Hamaïdia, Malik ULg

Poster (2013, July)

Glycans are macromolecules (O-glycosidic and N-glycosidic linkage of monosacharides) which can be extracted from various agricultural products (i.e. cellulose, lignin, starch, insect). Preliminary data ... [more ▼]

Glycans are macromolecules (O-glycosidic and N-glycosidic linkage of monosacharides) which can be extracted from various agricultural products (i.e. cellulose, lignin, starch, insect). Preliminary data indicate that glycans can stimulate inflammation without initiating an anti-inflammatory feedback loop. Notwithstanding, inhibition of immune response by anti-inflammatory cytokines is a major cause of cancer therapy failure in human. The goal of the project is to study the ability of glycans to modulate the functions of macrophages and dendritic cells in order to stimulate anti-tumor immune response. The project aims to assess this hypothesis in a high incidence cancer: Malignant Pleural Mesothelioma. Glycan extracts will be isolated from a variety of agroforestry resources and especially broadleaved trees of the different systems tested on the platform AgricultureIsLife (zones 1-3 : hedges, tree lines, agrisilviculture, short rotation coppice) and modified by physical, chemical, enzymatic or microbiotic approaches. [less ▲]

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See detailPerception of the Duration of time: a Developmental Semiology from 2:6 to 12 years, general population versus atypical, and elaboration of a parental questionnaire assessment
SCHOLL, Jean-Marc ULg; Delvenne, Véronique

Poster (2013, July)

Introduction The perception of time is usually considered from sequential time but there are not any semiological markers of the capacity to apprehend the duration of time. Hypothesis The “capacity to ... [more ▼]

Introduction The perception of time is usually considered from sequential time but there are not any semiological markers of the capacity to apprehend the duration of time. Hypothesis The “capacity to feel the passage of time” is determinable by “semiological markers” in everyday life. Method Elaboration of a parental questionnaire (Likert scale); 2 samples from 2:6 to 12 years: 827 in general population and 297 in transnosographic population with psychological therapy; logistic regression; percentiles curves. Results General population: perception of the passage of time is gradually acquired; full competence at 9 years. The apprehension of sequential time is quasi maximum at 4 years. Atypical population: perception of the passage of time delayed of 18 months; important difficulties remain at 13 years. The apprehension of sequential time is delayed; at 13 years it remains bad at percentile 95. No differences according to the sex. Presentation of a short questionnaire for assessment. [less ▲]

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See detailInternet-based physical activity promotion: Are older adults ready for this?
Mouton, Alexandre ULg; Cloes, Marc ULg

Poster (2013, July)

Despite the numerous health benefits, population physical activity (PA) levels are low and decline with age. In Belgium, people older than 60 years will represent more than a third of the entire ... [more ▼]

Despite the numerous health benefits, population physical activity (PA) levels are low and decline with age. In Belgium, people older than 60 years will represent more than a third of the entire population in 2050, with some major implications in terms of public health cost. Faced with this issue, innovative interventions to promote PA in older adults are of major interest. The Internet holds potential for delivering effective PA interventions. Besides, older adults are the largest-growing group of Internet users. Therefore, the main purpose of this study was to explore the opinion of older adults about Internet-based PA promotion. First, 75 older adults (73.75 ± 5.77 years) fulfilled a questionnaire about their PA stage of change, motivation about PA and opinion about several elements that could be included in an Internet-based PA website. Then, they were asked to participate in an unique session of “home exercising”. Finally, in a subsequent interview, they had to give their opinion about the inclusion of this kind of session in an Internet-based PA website. Only 48% of the participants met the official guidelines for PA. A regular access to the Internet was observed for 57.3% of the participants, with a significantly higher access for the younger ones (p = .014). Based on a 4-point Likert scale, the most popular topics for an Internet-based intervention were “PA social forum” (3.3/4), “PA local opportunities” (3.2/4), “PA local agenda” (3.1/4), “Benefits of PA” (3.1/4) and “PA programs” (3.1/4). According to the participants, the inclusion of “home exercising” in an internet-based PA website would be appreciated (7.85/10), adapted (8.78/10) and likely to be self-implemented at home (6.96/10). Findings suggest that an internet-based PA promotion may be acceptable for older people. Social and neighborhood environment elements appear to be essential in order to support the motivation for a regular PA practice. [less ▲]

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See detailDevelopment of an analytical strategy to measure major selenium-containing species in juvenile turtles (Trachemys scripta scripta) by SAX-HPLC-ICP MS
Far, Johann ULg; Dyc, Christelle; Das, Krishna ULg et al

Poster (2013, July)

Sea turtles are exposed to many environmental elements such as selenium (Se). Sea turtles are listed under the Red List of threatened species by the International Union for Conservation of Nature. It is ... [more ▼]

Sea turtles are exposed to many environmental elements such as selenium (Se). Sea turtles are listed under the Red List of threatened species by the International Union for Conservation of Nature. It is thus mandatory to use low-invasive tissue collection (skin, carapace, blood ) for estimating Se exposure in these highly protected turtles. For this purpose, a biological modal Trachemys scripta scripta (or slider turtle) was selected. For two months, juvenile turtles were dietary exposed to Se by spiking the food with Selenomethionine (SeMet) or Methionine as control groups. Individuals were sacrificed after different time of exposure and tissues (skin, liver, muscle, carapace and blood) collected to perform Se speciation and determine some biological endpoints. An analytical strategy was developed to cope with the very low amount of available sample. It is briefly consisting by reduction, alkylation and proteolysis of the entire freeze-dried tissues followed by sample clean-up using ultra-filtration membrane. Then anion exchange HPLC using salt and pH gradient was developed to prevent the introduction of organic solvents, which cause severe fooling of ICP MS and avoid ultra-trace analyses of sea water in routine analysis. This method successfully achieved the detection and quantification at ppm level of expected species (i.e SeMet, selenocysteine, inorganic Se) and also unknown species but their relative amounts were time and tissues dependent. [less ▲]

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See detailStudies on the Influence of Different Grain-sized Titania Scattering Layers for Dye Sensitized Solar Cells
Thalluri, Venkata Visveswara Gopala Kris ULg; Henrist, Catherine ULg; Vertruyen, Bénédicte ULg et al

Poster (2013, July)

The efficiencies of dye sensitized solar cells (DSCs) are boosted up to 12% by NIR light harvesting dyes and with the usage of scattering layer in the device preparation.The importance of Titania ... [more ▼]

The efficiencies of dye sensitized solar cells (DSCs) are boosted up to 12% by NIR light harvesting dyes and with the usage of scattering layer in the device preparation.The importance of Titania scattering layers was studied as a part of this work. These scattering layers were prepared from two different grain-sizes (100 nm & 500 nm) for SQ2-NIR and N3-UV/Vis DSCs. The 100 nm grain-sized Titania paste was commercially supplied and 500 nm grain-sized Titania paste was prepared according to literature. The morphological and structural properties of these bigger grain-sized Titania layers were deliberated by using and Scanning Electron Microscope (SEM) and X-Ray diffraction (XRD) measurements. The influence of these bigger grain-sized Titania scattering layers in SQ2-NIR and N3-UV/Vis DSCs were expounded by using various electro-optical characterization techniques such as light I-V, electrochemical impedance spectroscopy (EIS) shown in Figure 1 and external quantum efficiency (EQE) measurements. The importance of understanding the influence of these bigger grain-sized scattering Titania layers could pave a way for future design and optimizing of DSCs for increasing the amount of light harvesting. [less ▲]

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See detailInnovative analytical strategy using ion-mobility for structural or functional selenium isomers identification by ion mobility spectrometry
Far, Johann ULg; Kune, Christopher ULg; Lobinski, Ryszard et al

Poster (2013, July)

Selenium (Se) is a trace element which is both essential and toxic depending on its concentration and its chemical form. Se-rich yeast is one of the most popular Se source for supplementation. The ... [more ▼]

Selenium (Se) is a trace element which is both essential and toxic depending on its concentration and its chemical form. Se-rich yeast is one of the most popular Se source for supplementation. The classical method of speciation is related to multidimensional liquid chromatography (LC) hyphenated to mass spectrometry (MS) Recent advances in Se speciation led to greatly improve the Se speciation in these samples but isomers identification and quantification remain challenging. This work focuses on the elaboration of an innovative analytical strategy for the detection and the structural elucidation of isobaric selenium compounds present in Se-rich yeast. A specific complex formation agent acts as a chemical probe for the detection of chemical function. The addition of a complexing agent can improve the discrimination between structural or functional Se isomers using ion mobility techniques as Ion Mobility Spectrometry (IMS) by increasing the molecular weight (i.e. the m/z ratio in MS) and the collision cross section of a target ion after selective complexation. This Ion Mobility orthogonal separation improves the structural elucidation. Crown ethers used as shifting agents can specifically form complexes with primary amines. The addition of crown ether to different low molecular weight fractions obtained by multidimensional LC of a water extract from Se-rich yeast permitted to detect Se isomers and confirmed their structure using IMS. [less ▲]

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See detailAn interaction map for HTLV-1 Tax and PDZ-containing proteins.
Blibek, Karim ULg; Rambout, Xavier ULg; beaufays, Jérôme et al

Poster (2013, June 29)

Human T-cell leukemia virus type 1 (HTLV-1) retrovirus encodes for the Tax protein, which has a transforming capacity in vitro. Tax contains at its C-terminus a binding motif for PDZ domain-containing ... [more ▼]

Human T-cell leukemia virus type 1 (HTLV-1) retrovirus encodes for the Tax protein, which has a transforming capacity in vitro. Tax contains at its C-terminus a binding motif for PDZ domain-containing proteins (PSD95-DLG1-ZO1). It has been shown that the C-terminal motif of Tax is involved in Tax oncogenic capacity. Ten different PDZ domain-containing proteins have been reported to interact with Tax, but the specificity of Tax-human PDZome interactions has not been investigated. The objective of this study is to obtain a comprehensive interactome map for Tax and the human PDZome and to determine a global role of Tax-PDZ interactions in HTLV-1 biology. [less ▲]

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See detailInhibition of Tax transformation activity using a small molecule targetting Tax/PDZ domain interactions.
Blibek, Karim ULg; Fujii, Naoki; Legros, Sebastien et al

Poster (2013, June 29)

Primate T-lymphotropic virus species comprise four members (HTLV-1 to -4) that have been discovered in human. Only the HTLV-1 infection leads to adult T-cell leukemia/lymphoma (ATLL). All the four viruses ... [more ▼]

Primate T-lymphotropic virus species comprise four members (HTLV-1 to -4) that have been discovered in human. Only the HTLV-1 infection leads to adult T-cell leukemia/lymphoma (ATLL). All the four viruses share a similar genomic organization and encode transforming Tax oncoproteins. In contrast to HTLV-2 and 4, HTLV-1 and 3 Tax proteins contain a PSD-95/Drosophila Discs Large/Zona Occludens-I (PDZ) binding motif at their C-terminal that has been shown to play crucial roles in the distinct transforming properties of the Tax proteins. Here, we used a collection of human full-length protein-coding open reading frames (ORFeome v3.1) to identify novel PDZ domain containing proteins that specifically interact with HTLV-1 Tax. Novel Tax interactors include syntenin-1 and -2, LNX2, DVL3, GIPC2, INTU, PDLIM4 and -7, RADIL and RGS3. These proteins are involved in diverse biological processes including cell division, cell fate determination and cell suvival. We further characterized interaction between Tax and syntenins and showed that, FJ9 a small molecule able to disrupt Tax/PDZ interactions, could antagonize Tax-transformation activity in rat-1 model. Our study identify novel PDZ-containg proteins interacting with HTLV-1 Tax and provides the first example where Tax protein-protein interactions whith PDZ-containing proteins and Tax-transformation capacity could be inhibited by a small molecule. [less ▲]

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See detailInhibition of Tax transformation activity using a small molecule targetting Tax/PDZ domain interactions.
Blibek, Karim ULg; Fujii, Naoaki; Legros, Sebastien et al

Poster (2013, June 29)

Primate T-lymphotropic virus species comprise four members (HTLV-1 to -4) that have been discovered in human. Only the HTLV-1 infection leads to adult T-cell leukemia/lymphoma (ATLL) and tropical spastic ... [more ▼]

Primate T-lymphotropic virus species comprise four members (HTLV-1 to -4) that have been discovered in human. Only the HTLV-1 infection leads to adult T-cell leukemia/lymphoma (ATLL) and tropical spastic paraparesis (TSP), an immune degenerative neurologic syndrome. All the four viruses share a similar genomic organization and encode transforming Tax oncoproteins. In contrast to HTLV-2 and 4, HTLV-1 and 3 Tax proteins contain a PSD-95/Drosophila Discs Large/Zona Occludens-I (PDZ) binding motif at their C-terminal that has been shown to play crucial roles in the distinct transforming properties of the Tax proteins. To systematically investigate PDZ-containing proteins roles in HTLV-1 biology, we initiated a global interactome network analysis of Tax and associated human PDZ-containing proteins. This was accomplished through the use of our framework of binary interactome mapping that includes stringent yeast two hybrid and pulldown screening, systematic retesting by protein complementation assay and evaluation of PDZ gene expression in T lymphocytes. [less ▲]

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See detailEFFECT OF RAW MATERIAL PROPERTIES ON THE KINETICS OF IRON ORES GRANULATION
Jaimes Contreras, Rafael Antonio ULg; van Loo, Fréderic; Douce, Jean-François et al

Poster (2013, June 26)

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See detailTime Delays in Gravitationally Lensed Quasars
Eulaers, Eva ULg; Magain, Pierre ULg; Sohy, Sandrine ULg

Poster (2013, June 25)

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See detailImpacts of past Human disturbances on present day tree species assembly in the tropical forests of South-East Cameroon
Vleminckx, Jason; Doucet, Jean-Louis ULg; Daïnou, Kasso ULg et al

Poster (2013, June 25)

Non-random spatial distribution of trees is the result of both neutral and deterministic factors. Neutral models suggest that species within a community are equally competitive, with spatial structures ... [more ▼]

Non-random spatial distribution of trees is the result of both neutral and deterministic factors. Neutral models suggest that species within a community are equally competitive, with spatial structures mainly due to dispersal limitation. Deterministic (or non-neutral) models consider species assemblages as the result of what we name “induced spatial dependence”, where forcing (explanatory) variables shape diversity organization. However, deterministic models have often included habitat variables only, without considering human disturbance which we know enhances the competitive advantage of heliophytic (light-demanding) species and therefore the floristic composition of phytocenoses. Based on charcoal abundance in the soil (used as an indicator of anthropogenic perturbation), species abundance, and environmental data from a forest of south-east Cameroon, we applied modern variation partitioning methods to assess the relative impact of human disturbance on floristic patterns, controlling for purely spatial and habitat effects. Significant signals of human influence have been found so far, and a new collection of data should establish with a better precision the importance of the anthropogenic impact on tree species assemblages. [less ▲]

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See detailSpontaneous language of extremely premature children : Specific deficits in an informative language task
Martinez Perez, Trecy ULg; Grooteclaes, Vanessa; Docquier, Laurence et al

Poster (2013, June 24)

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See detailField investigation of subacute rumen acidosis prevalence in walloon dairy herds
Lessire, Françoise ULg; Knapp, Emilie ULg; Theron, Léonard ULg et al

Poster (2013, June 24)

Subacute ruminal acidosis (SARA) has been considered as a major metabolic disease in high producing dairy herds for years. However, different feeding practices However, different feeding practices and ... [more ▼]

Subacute ruminal acidosis (SARA) has been considered as a major metabolic disease in high producing dairy herds for years. However, different feeding practices However, different feeding practices and herd’s production levels are found in Wallonia. SARA prevalence in local herds was thus required 174 cows (164 Holstein and 10 Brown Swiss) out of 24 walloon herds were sampled from 2011 to 2012 for evaluation of their ruminal function. Selection of minimum 5 cows per herd was made on basis of days in milk (<150 DIM), or low milk fat % (F <3.2%), or fat/protein % (F/P≤1), or at farmer’s request (animal debilitated or chronically ill). Ruminal fluid was sampled 4-8 h after feeding using a Geishauser oro-pharyngeal probe, preventing saliva contamination. pH was measured by a portable pHmeter and values were reduced by 0.35 as proposed by Duffield (2004) because of the higher pH values in reticulum sampling site compared with rumenocentesis. Redox potential was determined by Methylene Blue Reduction Time (MBRT) and protozoa assessed by microscopy. Production values were obtained by the National Dairy Herds Improvement. Health scores were determined as described by Zaaier et al. (2001). RESULTS Mean production values ± SD of these animals (DIM: 106 ± 84) were 33.2 ± 8.9 kg milk, F = 3.47 ± 0.72%, P = 3.25 ± 0.25%, F/P = 1.07 ± 0.23. Mean BCS was 2.6 ± 0.6. Mean pH value ± SD was 6.50 ± 0.42. 10 animals (5.7%) from 6 herds were below 5.8, of which 4 cows from H8 (23 sampled cows). No result was < 5.5. MBRT was 4.16 ± 3.13 min. In 5 cows, value < 1min indicated a more amylolytic bacterial flora. In 24 samples, no reduction of MB occurred, demonstrating bacterial inactivity. Disappearance of large protozoa was observed in 6 samples, of which 5 abnormal specimens came from H8. No correlation between low pH values and F/P or %F could be found. CONCLUSION In no herd, prevalence was >25%, required for SARA diagnosis. Regarding these results, prevalence of SARA appears very low in Wallonia while on the opposite, ruminal flora inactivity seems far more common. [less ▲]

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See detailBiodegradable microspheres based on amphiphilic graft-copolymers of chitosan and polyesters
Demina, T; Drozdova, M.; Sevrin, Chantal ULg et al

Poster (2013, June 19)

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See detailGaining speed in molecular dynamics simulations by implicit representation of water and membrane molecules
Steinhauer, Sven ULg; Crowet, Jean-Marc ULg; Brasseur, Robert ULg et al

Poster (2013, June 19)

Molecular dynamics (MD) is an appropriate method for investigation of peptide-membrane systems and helps in analyzing results from experiments. In many cases, the ability of viral fusion proteins and ... [more ▼]

Molecular dynamics (MD) is an appropriate method for investigation of peptide-membrane systems and helps in analyzing results from experiments. In many cases, the ability of viral fusion proteins and toxins for destabilizing the membrane is due to their hydrophobic profile, leading to particular membrane insertion. By now, many relevant processes for drug design, toxicological studies and other fields of application, are not feasible by MD simulations, when each atom is represented over time. Processes such as protein folding, often take place above the time scales reachable by MD simulations, which are of the order of micro seconds. The necessary time effort for carrying out such simulations stays considerable and depends mainly on (1) the complexity of the simulated system (2) the simulated time scale (3) the simulation method (4) the efficiency of used hardware and software algorithms. Nowadays, MD simulations can still take weeks of calculation on high end computers. Impala is an implicit water and lipids forcefield, initially developed by our laboratory. Implicit forcefields replace water and/or lipid molecules by a couple of simple and partially precalculable equations. Using this method, thousands of water and lipid molecules can be replaced in MD simulations using Gromacs software. This leads to a considerable reduction of system complexity. The original Impala algorithm based on the assumption of rigid peptides and used a Monte Carlo algorithm with the aim of finding the insertion characteristics of these molecules in membranes. Our current work is the integration of the Impala forcefield into Gromacs, a freely accessible MD software. Replacing the aqueous and lipid phase atomic description in Gromacs MD by an implicit forcefield is supposed to lead to a gain of speed compared to full atomistic simulations. A gain of precision compared to Impala is expected, too. This will be achieved by turning molecules flexible, when implementing Impala into Gromacs. [less ▲]

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See detailOptimization of biodegradable microparticles for tissue engineering
Drozdova, M.; Demina, T.; Sevrin, Chantal ULg et al

Poster (2013, June 19)

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See detailCharacteristics of Pregnancy-Associated glycoprotein (PAG) like proteins in red deer
Okuyama, MW; Melo de Sousa, Noelita ULg; Beckers, Jean-François ULg

Poster (2013, June 19)

The Pregnancy-Associated glycoproteins (PAGs) constitute an asparatic proteinase family and are divided into 2 groups; bovine PAG-1 (boPAG-1) group and boPAG-2 group. PAGs have been detected in many ... [more ▼]

The Pregnancy-Associated glycoproteins (PAGs) constitute an asparatic proteinase family and are divided into 2 groups; bovine PAG-1 (boPAG-1) group and boPAG-2 group. PAGs have been detected in many domestic ruminant species and their biochemical characteristics were reported. However, information in wild ruminant, especially in deer species is scarce. The aim of this study is to investigate the characteristics of placental protein extracted in red deer (cervus elaphus); placental proteins which belong to each type of PAG group were identified in purification steps. Fresh frozen placenta (fetal cotyledon (FC): 2,247 g, maternal cotyledon (MC): 2,255 g respectively) was used as following purification step; protein extraction, acid precipitation (pH 4.5), Ammonium sulfate precipitation (SA0-40%, SA40-80%) and anion exchange chromatography (DEAE-cellulose 0M, 0,02M, 0,04M, 0,08M, 0,16M and 0,32M NaCl). In every step, Immunoreactivity against anti-PAG antisera was checked by Radioimmunoassay with using anti-PAG-1 antiserum (AS706) and anti-boPAG-2 antiserum (AS438). After Ammonium sulfate precipitation, much higher concentrations were gained in SA40-80% step (AS706: 5.35 mg/FCkg and 6.73 mg/MCkg, AS438: 24.79 mg/FCkg and 84.6 mg/MCkg) than SA0-40% step (AS706: 0.64 mg/FCkg and 0.79 mg/MCkg, AS438: 1.3 mg/FCkg and 9.34 mg/MCkg) in both part of cotyledon. After DEAE chromatography, the highest concentrations of protein against AS438 were gained in 0.08M NaCl step (5.49 mg/FCkg and 17.8 mg/MCkg) and concentrations of protein against AS706 were almost same in 0.08M NaCl (1.01 mg/FCkg and 1.35 mg/MCkg) and 0.16M NaCl (0.93 mg/FCkg and 1.34 mg/MCkg). In many ruminants such as cattle, sheep and goat, PAGs are contained largely in fetal cotyledon and most amounts of PAGs are belonging to PAG-1 group. On the other hand, in red deer, much larger amount of PAGs was obtained from maternal cotyledon. And stronger immunoreactivity with anti-boPAG-2 antisera was detected than with anti-PAG-1 antisera. This characteristic is similar to the results which were reported in pig and dromedary. Therefore, these results suggest that PAGs in red deer have different characteristics from other ruminant species. [less ▲]

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See detailMicroscopic techniques to study the interaction of new polymers within live cells
Smisdom, N.; Sanen, K.; Van Den Akker, N. et al

Poster (2013, June 19)

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See detailEnantioselective synthesis of thioesters as substrates for high-through put screening assays of Penicillin Binding Proteins
Simon, Justine ULg; Zervosen, Astrid ULg; Bouillez, André ULg et al

Poster (2013, June 19)

Excessive utilization of beta-lactam antibiotics like penicillin has created drug-resistant strains in bacteria. One of the main mechanisms of resistance is the production of drug resistant Penicillin ... [more ▼]

Excessive utilization of beta-lactam antibiotics like penicillin has created drug-resistant strains in bacteria. One of the main mechanisms of resistance is the production of drug resistant Penicillin Binding Proteins (PBPs) and the over expression of these proteins. The transglycosidase and transpeptidase activities of PBPs catalyze the last two steps of peptidoglycan biosynthesis, which is unique to bacteria, and lies outside the cytoplasmic membrane. PBPs are interesting targets and efforts are still done to find new inhibitors. <br />A thioesterase activity has been described for various PBPs. For example, the thioester S2d is a substrate of PBP R39 of Actinomadura and of PBP2x of Streptococcus pneumoniae. The utilization of thioesters allows a rapid screening of active compounds in high-through put screening assays. Furthermore detailed kinetic studies using thioesters as reporter substrates are also possible. <br />Here we will present the enantioselective synthesis of the thioesters and their application as substrates in high through put screening assays. [less ▲]

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