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See detailBiochemical methane potential of residues of three banana varieties from Cameroon : fresh and dry peduncles
Awedem wobiwo, Florent; Happi Emaga, Thomas; Fokou, Elie et al

Poster (2015, January 30)

In Cameroon , the ratio of energy cost to standard of living for both electric and fuel energy is higher than in USA and EU. The local energy needs could be met by valorizing local wastes in an ... [more ▼]

In Cameroon , the ratio of energy cost to standard of living for both electric and fuel energy is higher than in USA and EU. The local energy needs could be met by valorizing local wastes in an environmentally and economically sustainable way. Banana and plantain plants produce significant quantities of post-harvest biomass wastes such as bulbs, pseudo stems, leaf sheaths, petioles–midribs, leaf blades, peduncles, rachis and blossoms which can be converted to methane-rich biogas by anaerobic digestion (AD). AD is a natural process of degradation of organic materials. [less ▲]

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See detailNitrous oxide flux measurement with a closed chamber system : data treatment
Regaert, Donat ULg; Moureaux, Christine ULg; Heinesch, Bernard ULg et al

Poster (2015, January 30)

Nitrous oxide flux estimation from concentration measurements with a closed chamber system. Statistical data treatment to sort between relevant/irrelevant fluxes.

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See detail[18F]UCB-H as a new PET radiotracer for Synaptic vesicle protein 2A: A first clinical trial
Bahri, Mohamed Ali ULg; Stifkens, Mathieu; Bastin, Christine ULg et al

Poster (2015, January 27)

SV2A is widely distributed in the brain and has been demonstrated to be involved in vesicle trafficking. The critical role of SV2A in proper nervous system function is shown, e.g., by the fact that it is ... [more ▼]

SV2A is widely distributed in the brain and has been demonstrated to be involved in vesicle trafficking. The critical role of SV2A in proper nervous system function is shown, e.g., by the fact that it is a binding site and the primary mechanism of levetiracetam. Levetiracetam is an antiepileptic drug which has recently been suggested to reduce synaptic deficits in a mouse model for Alzheimer’s disease. We here aimed to investigate the cerebral distribution of [18F]UCB-H, which has a high affinity with the SV2A. Dynamic PET data of the head of 4 healthy volunteers were acquired over 100 minutes after injection of 170.4 ± 24.9 MBq of GMP produced [18F]UCB-H. The arterial input function (IF) was obtained by blood sampling. The IF was also derived from the dynamic data using the correlation coefficient method. Blood data revealed a consistent amount of [18F]UCB-H in whole blood and plasma indicating a very low degree of binding of the tracer to the red blood cells. The image-derived arterial IFs were showed to be very similar to the measured ones with a peak-ratio around 0.91 and an area-under-curve ratio about 0.98. The [18F]UCB-H PET data showed a high and rapid uptake in the grey matter structures, matching the known ubiquitous distribution of the SV2A in the brain. The kinetics of the tracer in the brain was characterized by an initial high uptake phase followed by rapid washout allowing the standard compartmental modeling (1-tissue, 2-tissue, and Logan Plot). The three models gave similar results with both the measured and image-derived IFs. The total distribution volume of the tracer in the brain was greater than 7 mL/cm3. Our results suggest that [18F]UCB-H is a good candidate as radiotracer for brain SV2A proteins and could be used for human studies. Image-derived IF showed to be useful for quantitative studies without the need to the arterial blood sampling. SV2A modifications may consequently be assessed in neurological pathologies such as Alzheimer’s disease. [less ▲]

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See detailIdentification of predictive markers based on functional imaging of metastatic spreading at the time of surgery after neoadjuvant radiotherapy
LALLEMAND, François ULg; Leroi, Natacha ULg; Bahri, Mohamed Ali ULg et al

Poster (2015, January 27)

Neoadjuvant radiotherapy (NeoRT) improves tumor local control and tumor resection in many cancers. The timing between the end of the NeoRT and surgery are driven by the occurrence of side effects or the ... [more ▼]

Neoadjuvant radiotherapy (NeoRT) improves tumor local control and tumor resection in many cancers. The timing between the end of the NeoRT and surgery are driven by the occurrence of side effects or the tumor downsizing. Some studies demonstrated that the timing of surgery and the RT schedule could influence tumor dissemination. Our aim is to evaluate with functional MRI the impact of the radiation treatment on the tumor microenvironment and subsequently to determine the best timing to perform surgery. We used a model of NeoRT, 4T1 cells were implanted in the flank of BalbC mice. Seven days after, tumors were irradiated with 2x5Gy than we surgically removed this lesion 11 days after RT. Diffusion Weighted (DW) and Dynamic Contrast Enhancement (DCE) -MRI was performed every 2 days during 11 days between RT and surgery. We developed a homemade “portacath” specifically dedicated for mice and for repetitive I.V. contrast agent injection. For DW-MRI, we performed sequences with 10 different B-value to achieve IntraVoxel Incoherent Motion analysis. For DCE-MRI, we used FSEMS sequence for keeping the same slices as with DW-MRI. For both images, we performed analysis on the entire tumor volume. We obtained very promising preliminary results showing good uniformity in the ADC (Attenuation Diffusion Coefficient). We succeeded to follow mice with imaging during the 11 days without major troubles. We observed less variability of the ADC signal during the 11 days in the irradiated tumors compared to the control. The signal to noise ratio was relatively poor for the diffusion sequence and need to be improved. For the first time, we demonstrate the feasibility of repetitive MRI functional imaging in a mice model of NeoRT. These results open perspectives for studying modifications of the tumor microenvironment induced by neoadjuvant RT. The techniques need to be improved and correlated to the tumor dissemination in function of the RT schedule and timing of surgery. [less ▲]

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See detailIdentification, Design and Evaluation of Pharmacological tools for the orphan GPCR GPR22
Geubelle, Pierre ULg; Gilissen, Julie ULg; Dupuis, Nadine ULg et al

Poster (2015, January 27)

GPCRs are the largest family of membrane receptors and are characterized by 7 transmembrane domains. GPR22 is a GPCR that has no known endogenous ligand and is thus considered "orphan". Its presence ... [more ▼]

GPCRs are the largest family of membrane receptors and are characterized by 7 transmembrane domains. GPR22 is a GPCR that has no known endogenous ligand and is thus considered "orphan". Its presence situated at the heart and brain levels makes it a potential target for new therapeutic pathways. This study consist in the identification of a synthetic ligand of GPR22 receptor to use it as a pharmacological tool in the study of the signaling channels of GPR22 in order to understand its role and to validate it as a new therapeutic target. The initial hypothesis was that GPR22 is coupled to the Gαi protein. [less ▲]

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See detailDéveloppement de nouveaux marqueurs neuroradiologiques de la maladie de Parkinson par reconnaissance de motifs
Himri, Khadidja ULg; Depierreux, Frédérique ULg; GARRAUX, Gaëtan ULg

Poster (2015, January 27)

Background and objectives: Automatic classification of Parkinson’s disease (PD) versus healthy controls (HC) based on structural MRI has so far focused on unimodal approaches. However, this method is ... [more ▼]

Background and objectives: Automatic classification of Parkinson’s disease (PD) versus healthy controls (HC) based on structural MRI has so far focused on unimodal approaches. However, this method is subject to a poor temporal and spatial resolution leading to low classification accuracy. To overcome this limitation we propose to integrate different modalities by generating a single decision function based on a multi-kernel method, exploiting the complementary information it offers. We predict that the integration of multiple modalities produces greater classification enhancement. Materials and methods: 3Tesla MRI was acquired in 42 patients with PD and 42 age and gender matched healthy controls. We relied on Unified Parkinson’s Disease Rating Scale (UPDRS) for evaluating the clinical status. We used structural and quantitative maps of T1, T2*, proton density (PD), magnetization transfer (MT), Multi-parameter (MT magnetization transfer, proton density (A), Iron Deposit (R2 *), mixing water content, iron, and the fraction of macromolecules tissues (R1) at 1 × 1 × 1 mm3 resolution. We identified cortical and subcortical brain regions (cortex, putamen, globus pallidus, substantia nigra), and cortical grey matter. We applied existing classification algorithms in the field of neuroscience using a classification algorithm based on Support Vector Machines (SVMs) [1], executed using the Pattern Recognition for Neuroimaging Toolbox (PRoNTo) [2]. The processes of classification was the following, data were mean centered and leave one subject out cross-validation was performed, making the test set independent from the training set. Analyses were restricted to voxels where all subjects had non-zero values. Statistical significance of the classifications was tested using permutation testing (1000 permutations) with random assignment of group class to the input image. Subsequently, we combined different modalities (MT, A, R1, R2) and identified the combination giving the highest sensitivity and sensibility in PD classification. As classifier we used support vector machines that are inspired by statistical learning theory Vladimir Vapnik and Multiple Kernel Learning approach, introduced by Lanckriet [3],[4]. Our approach can be seen as an analogue of MKL with SVMs. Conclusion & Future work: Identification of brain areas with affected intensity in the Parkinson’s group compared to Healthy Controls in single modalities using pronto is helpful. However, the subsequent multi-kernel approach utilizes unimodal information in a combined fashion so that emergent information is obtained, transcending effectiveness unimodal approaches. In conclusion, our findings suggest that combining different imaging modalities and different regions of interest increase classification accuracy significantly. These results are promising for objective diagnosis in medical practice. [less ▲]

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See detailBacterial DNA mimetics activate platelets and promote thrombosis via CLEC-2
Delierneux, Céline; Hego, Alexandre; Lecut, Christelle et al

Poster (2015, January 27)

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See detailLocalization of rDNA transcription sites within reptilian nucleoli
Bartholomé, Odile ULg; Franck, Claire; Thiry, Marc ULg

Poster (2015, January 27)

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See detailEffects of α-synuclein levels on cerebral synaptic function: Validation of a novel PET radioligand for the early diagnosis of Parkinson’s disease
Tarragon Cros, Ernesto ULg; Ferrara, André ULg; Tirelli, Ezio ULg et al

Poster (2015, January 27)

Background In Parkinson’s disease, converging evidence supports a pathogenic role for excessive α–synuclein accumulation in synaptic terminals that may propagate back to the soma of vulnerable nerve cells ... [more ▼]

Background In Parkinson’s disease, converging evidence supports a pathogenic role for excessive α–synuclein accumulation in synaptic terminals that may propagate back to the soma of vulnerable nerve cells such as neurons in the substantia nigra pars compacta. The resulting loss of dopaminergic terminals in the striatum can be demonstrated in vivo using 18F-Dopa-PET (positron emission tomography). However, there’s currently no validated biomarker of the progressive synaptic dysfunction in other vulnerable areas such as the cerebral cortex. Goal In this longitudinal study, we will test the hypothesis that the loss of synaptic terminals in a mouse model of excessive α–synuclein accumulation can be demonstrated in vivo before the occurrence of behavioural disturbances using 18F-UCB-H, a new PET biomarker developed at CRC. We will also test if this new imaging modality is sensitive enough to study the effect of a disease modifying therapy such as chronic physical exercise. Methods We will use microPET for the in vivo quantification of 18F-UCB-H brain uptake in 16 wild type animals and 16 transgenic (Tg) mice overexpressing human α–syn under the mThy1 promotor every 2 months. Data will be validated against post-mortem analyses after the last PET study. Predictions We predict decreased tracer uptake over time in the basal ganglia and cerebral cortex in Tg mice as compared with WT animals. Also, we predict a relationship between 18F-UCB-H uptake levels in basal ganglia and cerebral cortex and progressive alterations in both motor and cognitive functions, respectively. Further, we also expect that chronic exercise will slow down both motor and cognitive disturbances, as well as the rate of 18F-UCB-H brain uptake decreases. Conclusion If 18F-UCB-H PET proves to be a valid biomarker for the early detection of α–synuclein accumulation in the pre-clinical model of PD, the methods will tested on human clinical populations. [less ▲]

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See detailHuman cortical excitability depends on time awake and circadian phase
Gaggioni, Giulia ULg; Ly, Julien; Chellappa, Sarah Laxhmi ULg et al

Poster (2015, January 27)

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See detailAccumulation of methylglyoxal, a glycolysis by-product modulates YAP1 transcription co-factor localization and activity in human breast cancer cells through HSP90 modification
Nokin, Marie-Julie; Durieux, Florence; Peixoto, Paul et al

Poster (2015, January 27)

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See detailMyoferlin: an indispensable component in VEGR-A secretion by pancreatic cancer cell
Fahmy, Karim; Gonzalez, Arnaud; Arafad, Mohamed et al

Poster (2015, January 27)

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See detailRegulation of Tissue Factor by Epithelial-to-Mesenchymal Transitions: Impacts for the metastatic progression.
Francart, ME; Bourcy, Morgane ULg; Suarez-Carmona, M et al

Poster (2015, January 27)

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See detailMyoferlin regulates endosomal trafficking and tunes cancer cell metabolism
Blomme, Arnaud; Costanza, Brunella; Thiry, Marc ULg et al

Poster (2015, January 27)

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See detailßarrestin coupling of the orphan GPCR GPR27
Dupuis, Nadine ULg; Gilissen, Julie ULg; Derj, Anouar ULg et al

Poster (2015, January 27)

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2015, January 27)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glucose and glutamine. Indeed, interference with both glucose and glutamine supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose or glutamine deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detailExploration of volumetric cerebral changes, with de micro-MRi, due to psychomotor exercise in mice
Moës, Florian ULg; Plenevaux, Alain ULg; Becker, Guillaume ULg et al

Poster (2015, January 27)

It's well know that exercise is good for health .In addition exercise has postive effects on cognition ,neurodegenerative disease and on mood. Some studies show that exercise has effect on brain so the ... [more ▼]

It's well know that exercise is good for health .In addition exercise has postive effects on cognition ,neurodegenerative disease and on mood. Some studies show that exercise has effect on brain so the aim of this study is to see if there are volumetric changes due to exercise or not. [less ▲]

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See detailÉtude du Typus mélancholicus au sein d’une population de dépressifs unipoalires
ramackers, Amélia; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailÉtude du récit de vie de l’adulte à haut potentiel
Mormont, Elodie; Gauthier, Jean-Marie ULg; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailLa personnalité Borderline à travers le test de Rorschach
Rommes, Jennifer; Englebert, Jérôme ULg

Poster (2015, January 21)

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See detailCFD Large Eddy Simulation of the hydrodynamics of stirred mini-bioreactors operating with stem cell culture mixing conditions
Collignon, Marie-Laure ULg; Martin, Céline; Toye, Dominique ULg et al

Poster (2015, January 18)

The expansion of hMSC adhered on microcarriers is a proven technology to allow the production of quantitatively (cell density and growth rate) and qualitatively (preservation of cell stemness) the amounts ... [more ▼]

The expansion of hMSC adhered on microcarriers is a proven technology to allow the production of quantitatively (cell density and growth rate) and qualitatively (preservation of cell stemness) the amounts of cells required for clinical applications. In that context of process optimization, a platform of 6 parallel 250 mL stirred minibioreactors have been designed and fully equipped for hMSC cultivation on microcarriers. The local hydrodynamics in these bioreactors have been characterized using CFD simulations and experimental measurements. GOALS: 1) Evaluation of the flexibility of the platform for extensively studying the coupling between hydrodynamics and hMSC physiological response (growth, stemness) by changing agitation conditions. 2) Evaluation of the scale down ability of platform as prediction tool of the hMSC behavior in larger bioreactor scale. [less ▲]

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See detailThe Mediation Service, what interest for emergencies?
PIAZZA, Justine ULg; BRASSEUR, Edmond ULg; DOPPAGNE, Caroline ULg et al

Poster (2015, January 17)

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See detailCancer metabolism reprogramming in response to anti-angiogenic therapy
Sounni, Nor Eddine ULg

Poster (2015, January 15)

In this study, we have explored the tumor adaptation to antiangiogenic therapy with the multitarget receptor tyrosine kinase inhibitors (RTKIs) Sunitinib and sorafenib. These RTKIs are currently in ... [more ▼]

In this study, we have explored the tumor adaptation to antiangiogenic therapy with the multitarget receptor tyrosine kinase inhibitors (RTKIs) Sunitinib and sorafenib. These RTKIs are currently in clinical use for multiple tumor types and confer increased overall or progression-free survival. However, antiangiogenic drugs demonstrate only relatively modest survival benefits and transient responses to treatment. Furthermore, preclinical studies suggest that RTKIs withdrawal results in accelerated tumor progression and metastasis. By applying the RTKIs treatment to 5 preclinical models of cancer development with global screening technologies, we found that tumors shift their metabolism during antiangiogenic therapy and acquire more aggressive phenotype after treatment cessation. We found that accelerated tumor growth and metastasis upon RTKIs withdrawal was associated with decreased glucose metabolism, increased lipid metabolism, and activation of the tricarboxylic acid (TCA) cycle. In particular, withdrawal of RTKIs resulted in elevated levels of fatty acid synthase (FASN) and acetyl-CoA carboxylase and adipocyte accumulation in tumors. Antiangiogenic therapy induced a metabolic shift in cancer and stromal cells to a glycolytic and hypoxic state during treatment, which was reversed upon therapy withdrawal, resulting in a shift to de novo lipogenesis and increased TCA cycle activity to promote tumor regrowth. Targeting lipid metabolism using the FASN inhibitor orlistat or by specific knockdown of FASN suppressed the RTKIs withdrawal–associated tumor regrowth and metastasis. We will discuss the role for lipid metabolism in tumor adaptation to antiangiogenic therapy withdrawal. [less ▲]

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See detailFIRST, a fibered aperture masking instrument: Results of the Lick observing campaign
Bordwell, Baylee; Duchene, Gaspard; Huby, Elsa ULg et al

Poster (2015, January 01)

FIRST is a prototype instrument aimed at achieving high dynamic range and angular resolution in ground-based images at visible wavelengths near the diffraction limit. FIRST utilizes an aperture masking ... [more ▼]

FIRST is a prototype instrument aimed at achieving high dynamic range and angular resolution in ground-based images at visible wavelengths near the diffraction limit. FIRST utilizes an aperture masking-like technique that makes use of single-mode fibers and pupil remapping to maximize the area of the telescope mirror in use. While located at Lick observatory in 2011 and 2012, FIRST observed 25 binary systems with the Shane 3m telescope, with separations ranging from 20 to 200 mas, comparable to the 50 mas diffraction limit for our central wavelength. Huby et al. (2013) has reported results for the Capella system that established the utility of FIRST for characterizing stellar binaries using the directly measured spectral flux ratio. Using an improved data analysis pipeline, we obtained closure phase measurements for a majority of the targets observed at Lick, and derived angular separations and spectral flux ratios. From the spectral flux ratios we obtained spectra for the companions over at least 600-850 nm with R~300. Finally, by obtaining results for many binary systems we have better constrained the current performance of FIRST, which has an exciting future ahead at its current location behind SCExAO at the Subaru 8.2 m telescope, where it will eventually become available for general use by the astronomical community. [less ▲]

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See detailL'adolescent diabétique et la tentation suicidaire
Malchair, Alain ULg

Poster (2015, January)

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See detailChange in the protofilamentnumber and β5-tubulin appearance in supporting cells during development of the hearing organ.
Renauld, Justine ULg; Thelen, Nicolas ULg; Johnen, Nicolas ULg et al

Poster (2015, January)

The supporting cells of the hearing organ are characterized by the presence of an abundant cytoskeleton which is mainly composed of microtubules. These supporting cells have also been shown to contain a ... [more ▼]

The supporting cells of the hearing organ are characterized by the presence of an abundant cytoskeleton which is mainly composed of microtubules. These supporting cells have also been shown to contain a minor mammalian tubulin, the β5-tubulin, recently reported as a biomarker of cell proliferation. It was shown that a β-tubulin isoform can specify the microtubule architecture, as seen with the expression of the Moth β2 tubulin in the Drosophila testes which imposes the 16-protofilament (16pf) structure on the corresponding subset of Drosophila microtubules. Moreover, supporting cell microtubules are formed by 15pf instead of the canonical 13, a unique fact among vertebrates. Such a protofilament configuration has been observed in C. elegans’ neurons which are responsible for the mechanosensory sense of touch. It was also shown that these 15pf microtubules were essential to the proper functioning of these neurons. To determine the role of this particular tubulin in the auditory organ and its possible involvement in the formation of the unusual 15pf microtubules of supporting cells, we studied the spatiotemporal localization of β5-tubulin during development in rats from embryonic day 18 until P25 (25th postnatal day). Then we examined the fine structure of microtubules at the transmission electron microscope level (TEM). Our results showed that β5-tubulin, contrary to other β-tubulins, had a unique distribution in the cochlea. This β-tubulin appeared at a postnatal stage, before the opening of the Corti’s tunnel and is restricted to supporting cells, especially in pillar and Deiters’ cells. Our TEM study further indicated that these cells were composed by 13pf microtubules at P2, but by 15pf microtubules at P25. In conclusion, the architecture and composition of microtubules present in the supporting cells change during development of the Corti organ. Further experiments are now required to determine if these changes are related to the appearance of β5-tubulin. [less ▲]

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See detailCharacterization of the Chitin-Glucan Complex extracted from Komagataella Pastoris cell wall
Farinha, I; Duarte, P.; Pimentel, A. et al

Poster (2015)

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See detailLe portrait des agricultrices wallonnes en 2014
Dogot, Thomas ULg; Du Faux, Judith

Poster (2015)

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See detailAn unprecedented view of hot-star atmospheres from ASTRAL-II UV spectra: the early-types sample
Bianchi, L.; Ayres, T.; Hillier, D.J. et al

Poster (2015)

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See detailCerebellar hemorrhage : a rare condition in the term infant
MERINDOL, Ninon; BROUX, Isabelle ULg; DECORTIS, Thierry et al

Poster (2015)

Cerebellar hemorrhage is a rare condition in full-term newborns. Early diagnosis based on the identification of risk factors, particular clinical signs and correct medical imaging is primordial to ... [more ▼]

Cerebellar hemorrhage is a rare condition in full-term newborns. Early diagnosis based on the identification of risk factors, particular clinical signs and correct medical imaging is primordial to optimize the immediate treatment and to assess the long term prognosis. [less ▲]

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See detailDevelopment in Red River Delta, Vietnam
Nguyen Thi Minh Khué, ULg; Nguyen Thi, Dien; White, Ben et al

Poster (2015)

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See detailQuantitative Targeted Proteomics and Electrochromic Shift for Measuring Photosystem Content of Marine Phytoplankton
Malcolm, Christopher M.; Bailleul, Benjamin; Melanson, J.R. et al

Poster (2015)

Abundance and stoichiometry data for the photosystems, the intersystem electron transport complexes and the Calvin cycle enzymes are rich in information about light and nutrient acclimation. Quantifying ... [more ▼]

Abundance and stoichiometry data for the photosystems, the intersystem electron transport complexes and the Calvin cycle enzymes are rich in information about light and nutrient acclimation. Quantifying these complexes is essential for understanding limitations on and capacities for photosynthesis. Targeted quantitative immunodetections of conserved subunits (eg. PsbA for PSII; PsaC for PSI) are becoming an established method for absolute measurement of these complexes. An advantage of protein measurements is that they can be done with non-living flash-frozen samples and processed post-field. A pitfall of physical versus functional measures is that in some scenarios, such as during photoinhibition of photosystem II (PSII), physical and functional measures give different values, but such disparities are often meaningful, informing targeted studies of regulation, repair and enzyme kinetics. Electrochromic Shift (ECS) is an alternative, fast and noninvasive method which can be exploited to determine functional PSI:PSII ratios in living cells. The basis for ECS is that pigments in the photosynthetic membrane exhibit a shift in their absorption spectra when the electric component of the proton motive force is generated across the membrane in the light. Cross-validation of methods by independent measures builds confidence in results from both approaches and can be useful for ground truthing of underway or high-throughput optical measurements or functional measurements from bioassays. We present comparative data from immunoquantitation and ECS for an array of diatom taxa. The physical data fall within established ranges. The basis for similarities and disparities in the photosystem stoichiometries between the methods are discussed. [less ▲]

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See detailThe earliest pioneer settlement in Eastern Finland : the Sarvinki-project and the Jokivarsi 1 site
Simponen, Laija; Pesonen, Petro; Hertell, Esa et al

Poster (2015)

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See detailMachine Learning and Insulin Sensitivity in Determining Outcome in Preterm Infants
Uyttendaele, Vincent; Dickson, JL; Lynn, A et al

Poster (2015)

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See detailAssimilation of sea surface temperature, sea ice concentration and sea ice drift in a model of the Southern Ocean
Barth, Alexander ULg; Canter, Martin ULg; Van Schaeybroeck, Bert et al

Poster (2015)

Current ocean models have relatively large errors and biases in the Southern Ocean. The aim of this study is to provide a reanalysis from 1985 to 2006 assimilating sea surface temperature, sea ice ... [more ▼]

Current ocean models have relatively large errors and biases in the Southern Ocean. The aim of this study is to provide a reanalysis from 1985 to 2006 assimilating sea surface temperature, sea ice concentration and sea ice drift. In the following it is also shown how surface winds in the Southern Ocean can be improved using sea ice drift es- timated from infrared radiometers. Such satellite observations are available since the late seventies and have the potential to improve the wind forcing before more direct measure- ments of winds over the ocean are available using scatterometry in the late nineties. The model results are compared to the assimilated data and to independent measurements (the World Ocean Database 2009 and the mean dynamic topography based on observations). The overall improvement of the assimilation is quantified, in particular the impact of the assimilation on the representation of the polar front is discussed. Finally a method to iden- tify model errors in the Antarctic sea ice area is proposed based on Model Output Statistics techniques using a series of potential predictors. This approach provides new directions for model improvements. [less ▲]

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See detailDetermination of absolute molecular weight of bioactive polycations
Plotnikova, Evgeniya ULg; Sevrin, Chantal ULg; Claes, Nicolas et al

Poster (2015)

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See detailGenetic study of triple negative breast cancers
Boukerroucha, M; Josse, Claire ULg; El Guendi, Sonia ULg et al

Poster (2015)

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See detailVolatile organic compounds emitted by Cavendish and Plantain banana plants
De Clerck, Caroline ULg; Kherkhofs, Celine; Berhal, Chadi et al

Poster (2015)

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See detailIn vitro degradation study of Polydioxanone (PDO) monofilaments
Sevrin, Chantal ULg; Lombart, François ULg; Kottgen, Cindy et al

Poster (2015)

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See detailLaboratory findings suggesting an association between BoHV-4 and bovine abortions in southern Belgium
Delooz, L; Czaplicki, G; Houtain, JY et al

Poster (2015)

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See detailVery high resolution surface mass balance of Svalbard with the regional climate model MAR
Lang, Charlotte ULg; Fettweis, Xavier ULg

Poster (2015)

We present climate and surface mass balance (SMB) results over Svalbard simulated by a new version of the regional climate MAR model allowing to reach ∼km resolution without highly time-consuming runs ... [more ▼]

We present climate and surface mass balance (SMB) results over Svalbard simulated by a new version of the regional climate MAR model allowing to reach ∼km resolution without highly time-consuming runs. Spitsbergen, the largest island of the Svalbard archipelago, has a very hilly topography and, as the SMB strongly depends on the local topography and ice distribution, we need a high spatial resolution to accurately represent the SMB of Svalbard and its complex spatial distribution. However, higher resolution simulations are also very time consuming. That is why we have developed a new version of the MAR model in which the atmospheric module runs at a resolution of 7.5 km and the snow/ice module runs at a resolution of 3.75 km. Simulations over 1960-2014 forced by ERA show better agreement with SMB observations compared to our recent 10 km resolution recently published results. [less ▲]

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See detailEvaluation du débit de filtration glomérulaire en population générale : résultats en Côte d’Ivoire E.
Yayo, E; Konan, JL; Aye, M et al

Poster (2015)

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See detailPOLYMER BIODEGRADABLE MATRICES WITH ENHANCED SURFACE PROPERTIES FOR TISSUE ENGINEERING
Markvicheva, Elena; Drozdova, Maria; Demina, Tatiana et al

Poster (2015)

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See detailTotal connectivity: a marker of dynamical functional connectivity applied to consciousness
Liegeois, Raphaël ULg; Phillips, Christophe ULg; Bahri, Mohamed Ali ULg et al

Poster (2015)

In the last years functional connectivity (FC) has become one of the most popular tools to explore and characterize information contained in fMRI =me series. The classical hypothesis on FC consists of ... [more ▼]

In the last years functional connectivity (FC) has become one of the most popular tools to explore and characterize information contained in fMRI =me series. The classical hypothesis on FC consists of considering it as constant (or static) over the whole fMRI time series. However, it has been emphasized recently that FC should be treated as a dynamical quantity, for example by using sliding windows of the fMRI time courses in order to compute a dynamical FC. We propose a comprehensive marker of FC based on an auto-regressive (AR) model of fMRI time series capturing its static and dynamic properties. We call it total connectivity and we illustrate the benefits of our approach on data of patients undergoing four different states of consciousness. [less ▲]

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See detailMT4-MMP (MMP17) as a therapeutic target in metastatic solid tumors
Noël, Agnès ULg

Poster (2015)

Others and we have identified the glycosylphosphatidyl inositol (GPI) anchored MMP called MT4-MMP or MMP-17 as a main driver of cancer cell metastasis in breast (1-3), head and neck (4) and colon cancers ... [more ▼]

Others and we have identified the glycosylphosphatidyl inositol (GPI) anchored MMP called MT4-MMP or MMP-17 as a main driver of cancer cell metastasis in breast (1-3), head and neck (4) and colon cancers (5). More recently, we demonstrated a functional link between MT4-MMP and EGFR signaling in promoting breast cancer cell proliferation in vivo and in 3D culture models. We found that MT4-MMP is a key partner of EGFR activation and signaling (6), which is independent of its enzymatic activity. In triple negative breast cancer (TNBC) cells, MT4-MMP promotes primary tumor growth and lung metastases (1,2). Herein, we investigated the clinical relevance of our finding by immunohistochemical (IHC) study of MT4-MMP and EGFR expression in human samples of several breast cancer subtypes including lobular and ductal carcinoma in situ, invasive carcinoma, triple negative ductal carcinoma and normal tissue. IHC staining of normal breast tissues with MT4-MMP antibody show no signal, whereas breast carcinomas are positives for MT4-MMP. Of note, a strong staining for MT4-MMP was observed in the triple negative breast cancer (TNBC), the most aggressive breast cancer subtype. TNBC are known to express high level of EGFR and treatment options are limited due to the non-response of the TNBC patients to the EGFR targeted therapy. By investigating 86 TNBC tumors, we found a strong correlation between MT4-MMP and EGFR expression in a 68 % of TNBC tumors. In parallel to the human study, by exploring the mechanism of MT4-MMP trafficking and internalization, we found for the first time that MT4-MMP can be recycled at the cell surface. Mechanistically, MT4-MMP uses a unique endocytic pathway, which relies on CLIC/GEEC route. In addition to deeply investigating its internalization dynamics, we also explored the mode of its oligomerization and dimerization at the cell surface. Altogether, we are providing mechanistic insights on how MT4-MMP availability is regulated and revealing unique features of this MT-MMP among other MT-MMPs. These findings can be useful for designing therapeutics to block its presence at the cell surface, rather than merely blocking its enzymatic activity to block its pro-tumor effects. (1) Chabottaux et al., 2006, Cancer Res 66, 5165-5172; (2) Host et al., 2012, Int. J. Cancer 131(7):1537-48; (3) Rizki et al., 2008, Cancer Res 68, 1378-1387), (4) Huang et al., 2009, Neoplasia 11, 1371-1382) (5) Nimri et al., 2013, Mol Carcinog 52, 859-870 (6) Paye et al., 2014, Cancer Res. 74(23):6758-70 [less ▲]

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See detailA dynamic Unfolded Protein Response controls cortical neurogenesis
Creppe, Catherine ULg; Laguesse, Sophie; Nedialkova, Dany et al

Poster (2015)

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See detailAn empirical classification scheme for detection of impossible and improbable CRS-R subscore combinations
Chatelle, Camille ULg; Bodien, Yelena Guller; Carlowicz, Cecilia et al

Poster (2015)

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See detailVariation de la parathormone et de différents biomarqueurs osseux chez le patient hémodialysé
DELANAYE, Pierre ULg; Warling; Moonen, M et al

Poster (2015)

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See detailA retrospective study on Equine Herpes Virus type-1 associated myeloencephalopathy in France (2008-2011)
van Galen, G; Leblond, A; Tritz, P et al

Poster (2015)

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See detailRole of Kinases in VZV Nuclear Egress
Blondeau, Caroline ULg; Istaz, Bastienne; Lebrun, Marielle ULg et al

Poster (2015)

Like all Herpesviruses VZV expresses two conserved proteins forming the nuclear egress complex (NEC), namely ORF24p and ORF27p, respectively homologous to UL34 and UL31 in HSV-1. Many works described the ... [more ▼]

Like all Herpesviruses VZV expresses two conserved proteins forming the nuclear egress complex (NEC), namely ORF24p and ORF27p, respectively homologous to UL34 and UL31 in HSV-1. Many works described the role of this complex and viral kinases, the Alpha-herpesvirus specific kinase US3p, and hCMV UL97p (conserved in all Herpesviruses and homolog to UL13p in HSV-1), in the nuclear capsids translocation to the cytoplasm. Indeed, using a very complex mechanism, not yet fully characterized, nuclear capsids bud through the internal nuclear membrane, stay briefly in the perinuclear space and finally free the capsids into the cytoplasm through fusion of the primary envelope with the external nuclear membrane. In the cytoplasm, virions acquire the second and last envelopment before being released outside the cells. In order to better characterize the nuclear egress in the context of VZV, we focused our work on the NEC and on both viral kinases ORF47p and ORF66p respectively homologous to HSV-1 UL13p and US3p. By co-immunoprecipitation we identified a protein complex containing ORF24p, ORF27p and both viral kinases and are currently trying to determine the role of each kinase in the formation of this complex. We are also characterizing the phosphorylation status of these proteins depending on ORF66p and/or ORF47p. Immunofluorescence studies of cells infected with a deleted ORF66 or ORF47 virus showed mislocalisation of some viral proteins such as ORF24p as described for HSV-1. Finally electron microscopy analyses are in progress and will help to determine the role of both VZV kinases in the nuclear egress. [less ▲]

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See detailBridging familiarity-based recognition memory and novelty detection: A matter of timing
Delhaye, Emma ULg; Bastin, Christine ULg; Moulin, Christopher et al

Poster (2015)

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