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See detailLong term stability of TiO2 templated multilayer films used as high efficiency photoelectrode in liquid DSSCs
Dewalque, Jennifer ULg; Nguyen, Ngoc Duy ULg; Henrist, Catherine ULg et al

Poster (2013, March)

To our knowledge, the stability results reported in the literature only concern cells made from classical doctor-bladed or screen-printed nanoparticles films. This study focuses on the comparison of the ... [more ▼]

To our knowledge, the stability results reported in the literature only concern cells made from classical doctor-bladed or screen-printed nanoparticles films. This study focuses on the comparison of the long-term stability of these cells with DSSCs working with templated mesoporous films. Indeed, the increased surface area of templated films could lead to a faster degradation of the resulting cells. In accordance with IEC:1646:1996 standard tests, light soaking test at 45°C has been applied to determine the cells stability under prolonged illumination. Moreover, thermal stress in the dark has been applied. Unfortunately, due to the sealing material heat resistance, thermal stress test was only performed at 45°C. [less ▲]

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See detailSAHBEN, an accessible surface-based elastic network to insert a protein in a complex lipid membrane
Dony, Nicolas ULg; Crowet, Jean-Marc ULg; Joris, Bernard ULg et al

Poster (2013, February 26)

Study of membrane proteins have become one of the most challenging fields in biology. Solving their structure is one important step toward the understanding of their physiological activity but despite the ... [more ▼]

Study of membrane proteins have become one of the most challenging fields in biology. Solving their structure is one important step toward the understanding of their physiological activity but despite the recent advances in membrane protein crystallization, it represents less than 1 % of the entries in the Protein Data Bank. Therefore, calculation methods to study membrane proteins are helpful to complement experimental studies and fill the gap between the information obtained from the sequence and/or structure, the experimental results and the biological activity. Molecular Dynamics (MD) is a method of choice for membrane simulations and the rising of coarse-grained forcefields has opened the way to longer simulations with reduced calculations times. However, these approaches have two main drawbacks, the preparation of the membrane system and the preservation of the 3D protein structure, which is not trivial in CG approach. To circumvent these problems, we propose to use a modified version of the Gromacs tool genbox to easily insert lipids and a network based on hydrogen bonds and accessible surface to maintain the protein 3D structure. This protocol is available through a website (gcgs.gembloux.ulg.ac.be). [less ▲]

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See detailPrediction of membrane protein structures and TM interactions Rosetta and molecular dynamic studies
Crowet, Jean-Marc ULg; Dony, Nicolas ULg; Joris, Bernard ULg et al

Poster (2013, February 26)

The structures of membrane domains of the Divisome proteins and BlaR are not known and there is no homolog proteins of known structure to build homolgy models. Although the structure prediction of ... [more ▼]

The structures of membrane domains of the Divisome proteins and BlaR are not known and there is no homolog proteins of known structure to build homolgy models. Although the structure prediction of membrane proteins seems easier than for globular proteins, their ab initio prediction remains a difficult task. Only few methods have been used and validated on experimental pdb structures. By using the MARTINI or Bond coarse grain representation, the multimerization of transmembrane helices has been carried out by molecular dynamics, and the structure of several membrane proteins has been predicted by a tool of the Rosetta package. These methods are used here to predict the structure of the membrane embedded part of the politopic proteins from the divisome (FtsW, FtsK, FtsX and MraY) and BlaR. In a following part the MARTINI force field can be used to predict the TM helices interactions between the Divisome protein members. [less ▲]

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See detailComplex regulation of the FRD3 gene in Arabidopsis relatives
Charlier, Jean-Benoit ULg; Polese, Catherine ULg; Nouet, Cécile ULg et al

Poster (2013, February 26)

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See detailTreasures from the cave – mining for actinomycetes diversity
Maciejewska, Marta ULg

Poster (2013, February 26)

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See detailIn silico study of antimicrobial cyclic peptides Sequence analysis, molecular modelling and multi-scale molecular dynamics
Crowet, Jean-Marc ULg; Soumillion, Patrice; Brasseur, Robert ULg et al

Poster (2013, February 26)

The selection and use of antimicrobial cyclic peptides is an active way of research. These peptides are naturally produced by several microorganisms et libraries of biosynthetic peptides are actually ... [more ▼]

The selection and use of antimicrobial cyclic peptides is an active way of research. These peptides are naturally produced by several microorganisms et libraries of biosynthetic peptides are actually build to find new antibiotics candidats. However, the mecanism of action of these peptides is not well known and it exists several hypothesis for their interactions with membrane. These peptides are causing broad perturbations to lipidic membranes and it has been shown that they can form disordered toroidal pores or self assemble as amphipathic nanotubes leading to lipid extrusion. Through the analysis of several peptides from the libraries of Pr Soumillion with increasing activity it will be possible to study the relation between the sequence/structure and the membrane activity of these peptides. This will help to decipher between preferential modes of action and the parameters important for the activity. [less ▲]

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See detailBiochemical and Structural studies of the type I tagatose bisphosphate aldolases
Freichels, Régine ULg; Guarino, Carla; Delmarcelle, Michaël ULg et al

Poster (2013, February 26)

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See detailCharacterization of monoclonal antibodies against Hemolysin Alpha of Staphylococcus aureus
Crasson, Oscar ULg; Rhazi, Noureddine; Kinet, Marie et al

Poster (2013, February 26)

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy ... [more ▼]

In this study, vaccine-based targeting of Hemolysin Alpha (HLA) provides protection against lethal staphylococcal infection in a murine model system, suggesting that a monoclonal antibody-based therapy may likewise prove to be efficacious for prevention and treatment of this infection. We report the generation of four distinct anti-α-hemolysin monoclonal antibodies (MAbs) that antagonize toxin activity in vitro and protecting experimental animals against lethal S. aureus infection. [less ▲]

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See detailCase management projects enabling frail older people to stay in their own home a nested analysis within the framework of the evaluation of Protocol 3 projects
Van Durme, Thérèse; Macq, Jean; Lopez Hartmann, Maja et al

Poster (2013, February 22)

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See detailAn occupational therapist as a new professional at home: which profiles of clients are reached ?
Maggi, Patrick ULg; de Almeida Mello, Johanna; Delye, Samuel ULg et al

Poster (2013, February 22)

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See detailSTUDY OF THE EFFECT OF TWO SURFACTANTS ON SPRAY RETENTION BY BARLEY LEAVES
Boukhalfa, Hassina dite Hafida ULg; Massinon, Mathieu ULg; Lebeau, Frédéric ULg

Poster (2013, February 08)

Surfactants are nowadays very useful additives to improve the effectiveness of phytosanitary treatments. They contribute to change the types of impact and thus the amount of spray retained by the leaves ... [more ▼]

Surfactants are nowadays very useful additives to improve the effectiveness of phytosanitary treatments. They contribute to change the types of impact and thus the amount of spray retained by the leaves of the treated plant. We performed tests of retention on whole barley plants on BBCH 12 stage and small pieces of barley leaves at the same stage of growth. Spraying was done in three ways: water without surfactant, water with Break-Thru S240 and water with Li700. The three slurries of fluorescein contained in an amount of 0.2 g / l. Fluorescein retained by the leaves in both cases is then measured by a spectrofluoremeter. The retention tests on whole plants show that it is tripled by the first surfactant and doubled by the second. By cons on small pieces of barley leaves, the amount was increased by the use of surfactants but not to the same scale. This study concluded that the use of surfactants in spray pesticides may increase the amount of retention as a function of leaf area and the surfactant used. [less ▲]

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See detailSURVEY OF RICE DISEASES IN THREE REGIONS IN MADAGASCAR
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Zalmine, Francia et al

Poster (2013, February 08)

Rice (Oryza sativa) is the most cultivated crop and the staple food in Madagascar. However, the country has never achieved food self-sufficiency and continues to import rice abroad. Rice diseases are one ... [more ▼]

Rice (Oryza sativa) is the most cultivated crop and the staple food in Madagascar. However, the country has never achieved food self-sufficiency and continues to import rice abroad. Rice diseases are one of the factors limiting rice production and causing significant yield reductions. Our study aims to identify the rice pathogens present in three region of Madagascar: two of these regions (Analamanga and Vakinankaratra) are located in upland region when the last (Atsinanana) is located in coastal regions. Fifty five (55) rice samples were collected and cultured on standard media. A survey questionnaire was also conducted with the farmers in order to collect information about the context in which the diseases appeared. Micro-organisms isolated were identified by morphological characteristics for fungi, gram stain and anaerobic growth test for bacteria. In addition, molecular DNA characterization was performed by PCR using universal primers for both fungi and bacteria. The amplicons obtained were sequenced and analyzed by BLAST. In Analamanga and Vakinankaratra, Alternaria sp. and Xanthomonas oryzae pv. oryzae were pre-dominant. In the Atsinanana region, however, Curvularia lunata and Pseudomonas fuscovaginae were the most present. The weather in upland regions is indeed warm and humid in summer, dry and cold in winter which favours Xanthomonas oryzae pv. oryzae and Alternaria sp.growth. Moreover, irrigation is practiced in those regions which can explain the spread of the diseases. The weather in coastal region is warm and humid all year long which tend to favor weed development all year, serving as alternative hosts for Pseudomonas fuscovaginae and Curvularia lunata in interseason. [less ▲]

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See detailCHARACTERIZATION OF AGRICULTURAL SPRAY BY DIGITAL ANALYSIS OF SHADOWGRAPHY IMAGES
De Cock, Nicolas; Massinon, Mathieu ULg; Lebeau, Frédéric ULg

Poster (2013, February 08)

Agricultural sprays are among the most common two-phase flows studied because their characteristics determine the efficiency of treatment as well as environmental contamination. At present, the relevance ... [more ▼]

Agricultural sprays are among the most common two-phase flows studied because their characteristics determine the efficiency of treatment as well as environmental contamination. At present, the relevance of different characterization techniques of the highly polydispersed sprays used in this application remains controversial. Digital analysis of shadowgraphy images presents an attractive option for the characterization of both velocity and size of droplets present in the spray. This study presents an overview of the contrast problems inherent in the use of backlighted images and propose appropriate solution to ensure the quality of measurements. Generally, background light presents heterogeneities associated with light sources and optical arrangement. These can be solved by substracting from each images a composite background. An other particular focus is given to one major drawback of volumetric lighting, the presence of out focus droplets. These droplets have to be removed during the image analysis process because the measurement of their diameters can't be done accuratly. The rejection of these out of focus objects is based on a quantitative parameter which was calibrated with a obliquely shot monodispersed spray. The final step of the image processing is determining velocity of the droplet by tracking a same droplet on two successive images. The tracking algorithm is based on the size of the droplet, its more probable displacement and its direction. [less ▲]

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See detailEvaluation of the volatile emission changes from Arabidopsis thaliana Col-0 in response to temperature stress and Myzus persicae infestation interaction by HS-SPME-GC/MS
Hien, Truong Thi Dieu ULg

Poster (2013, February 08)

Volatile organic compounds (VOCs) are considered to be priming agents in plant responsive defense to protect themselves against abiotic or/and biotic stresses. Such stresses often influence on plant ... [more ▼]

Volatile organic compounds (VOCs) are considered to be priming agents in plant responsive defense to protect themselves against abiotic or/and biotic stresses. Such stresses often influence on plant photosynthesis and defense responses, resulting in a variety of volatile profiles. We investigated how different temperature regimes affect the VOCs emission capacity of Arabidopsis thaliana Col-0 (A.t Col-0) in the presence and absence of a sucking insect – Myzus persicae (green peach aphid). VOCs analyses were made with solid-phase micro-extraction coupled with gas chromatography - mass spectrometry (SPME- GC/MS) under controlled and various stress treatments. In response to temperature stresses, alcohols, ketones, aldehydes and terpenes were the most emitted VOCs. Moreover, the compared results showed that the percent emission of several compounds changed significantly. The interaction between temperature stresses and aphids released some new volatile components; like isothiocynates, esters, sulfur compound and nitrile. Besides, the qualitative and overall proportion of volatile blends differed significantly from plants subjected to the stress treatments within different time intervals (0-24h; 24-48h and 48-72h). Overall, both stress treatments correlated with increased or decreased levels of VOCs classes from Arabidopsis shoot organs. Knowledge of how the temperature – aphids interaction influences on VOCs emission in this study also provides interesting information for evaluating the responsive resistance in plants under natural environments. [less ▲]

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See detailImpact of the depth on bacterial diversity in an agricultural soil
Stroobants, Aurore ULg; Degrune, Florine ULg; Lambert, Christophe et al

Poster (2013, February 08)

Bacteria are the most abundant and diverse microorganisms in soils. They play an important role in soil formation, contribute to plant nutrition and are involved in various processes in agroecosystems ... [more ▼]

Bacteria are the most abundant and diverse microorganisms in soils. They play an important role in soil formation, contribute to plant nutrition and are involved in various processes in agroecosystems such as nutrient cycling. The aim of this study was to evaluate the impact of the depth on bacterial diversity and quantity in an agricultural soil. Samples was collected on May 2011 and May 2012 at three different depths : 10, 25 and 45 centimeters. The quantity of total bacteria was measured by real time PCR and the analysis of the diversity was performed by the high throughput sequencing technology. Results obtained by these methods show that the biomass and the bacterial quantity and diversity (Shannon index) decrease with the depth, particularly at 45 centimeters. The biomass is, in average, 6.5 fold less important at 45 cm than at 10 cm and the quantity is 17 fold lower at 45 cm than at 10 cm. Our results also indicate that many taxa, such as Betaprotebacteria, Deltaproterobacteria, Gammaproteobacteria, Acidobacteria and Burkholderiales are influenced by the depth. The results will be presented in more details on the poster. [less ▲]

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See detailInfluence of rhizobacterial volatile compounds on growth and root system architecture of Brachypodium distachyon (L.) Beauv.
Mendaluk, Magdalena ULg; Varin, Sébastien; Baudson, Caroline ULg et al

Poster (2013, February 08)

Many rhizobacterial strains are classified as plant growth-promoting rhizobacteria (PGPR) and are potentially useful to enhance plant fitness and productivity. Among the mechanisms by which PGPR improve ... [more ▼]

Many rhizobacterial strains are classified as plant growth-promoting rhizobacteria (PGPR) and are potentially useful to enhance plant fitness and productivity. Among the mechanisms by which PGPR improve plant growth, the emission of volatile organic compounds (VOCs) and their biological impacts on plants remain little documented. The aim of this work is to evaluate the growth promotion ability of 19 bacterial strains on the model grass Brachypodium distachyon Bd21 (Bd21), with a focus on this peculiar mode of interaction. A collection of 19 strains was selected for their known growth promotion potential. The impact of the rhizobacteria on the model grass was studied using an in vitro cocultivation system allowing interactions through VOCs without physical contact between plants and bacteria. The results show that the VOCs of several strains promote total biomass production, modulate biomass partitioning and affect shoot and root architecture of Bd21. The effects of the PGPR strains emitting different volatile blends were investigated via principal component analysis coupled to clustering and univariate analysis to unravel their biological effects. On the basis of volatile emissions and of their effects on plant growth, two strains have been selected in order to evaluate such interactions using an ex vitro system. The set-up of this system is in progress. [less ▲]

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See detailSurvey of rice diseases in three regions in Madagascar
Razanakoto Mamiharisoa, Lea; De Clerck, Caroline ULg; Zalmine, F. et al

Poster (2013, February 08)

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See detailInfluence of drying and hydrothermal treatments on the denaturation of corn proteins
Odjo, Djosse Psijus Sylvanus ULg; Malumba Kamba, Paul ULg; Bera, François ULg

Poster (2013, February 08)

Corn (Zea mays L.) is the most abundant cereal grain produced in the world. It also provides a significant amount of protein in human and animal diets. During drying corn grains undergo several ... [more ▼]

Corn (Zea mays L.) is the most abundant cereal grain produced in the world. It also provides a significant amount of protein in human and animal diets. During drying corn grains undergo several alterations including protein denaturation. A series of studies have been conducted in order to understand mechanisms behind denaturation of corn proteins during drying and heat moisture treatments. Using a laboratory fluidized-bed dryer, a flint corn variety have been dried between 54° and 130°C and subjected to different analyses from a qualitative point of view with a sequential extraction of corn protein fractions and from a quantitative point of view with the modeling of salt-soluble proteins using Promatest assay. Albumin, globulin and zein solubilities dropped significantly when the drying temperature increased. The electrophoretic patterns of zein and glutelin-G1 were not significantly modified, although the solubility of zein was affected by the drying temperature. The analysis of the different protein group solubilities suggested that denaturation mechanisms other than the creation of new disulfide bonds occurred during the drying of corn at high temperature. The study of the effect of heat treatments on the extractability of salt-soluble proteins shows that temperature; moisture content and time of processing greatly influence their kinetic denaturation. Modeling of salt-soluble proteins reveals that the evolution of extractible salt-soluble proteins content of corn kernels during drying at high temperature is more correctly described with a second order kinetic reaction. Investigations continue in order to understand protein denaturation and influence in functional properties and end-use of corn protein-based products. [less ▲]

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See detailIs ultra-short cold ischemia the key to IBDL avoidance in DCD-LT?
DETRY, Olivier ULg; DE ROOVER, Arnaud ULg; Ledinh, Hieu et al

Poster (2013, February 08)

Detailed reference viewed: 26 (6 ULg)
See detailTowards a characterisation of iron oxide-rich rocks used during the Archaic period on the Costa Arreica in Northern Chile
Salomon, Hélène ULg; Coquinot, Yvan; Salazar, Diego et al

Poster (2013, February 07)

Iron or manganese oxide rich rocks were constantly and intensively used by hunter- gatherers from around 300 kya. Nonetheless, few is none concerning the supply in raw ferruginous materials. The mine San ... [more ▼]

Iron or manganese oxide rich rocks were constantly and intensively used by hunter- gatherers from around 300 kya. Nonetheless, few is none concerning the supply in raw ferruginous materials. The mine San Ramón 15 in northern part of the Chilean coast reveals an exceptional evidence of the extraction of iron and probably manganese oxide rich materials by groups of hunters-fishers-gatherers. Two extraction phases were determined during the excavation of the mine trench: the oldest one during the Pleistocene-Holocene transition (11000-8000 cal. BP) and the second one from 4300 cal. BP. A rich set of lithic pounding stones and hammer stones were recorded in the mine refus. The mine trench follows a various meters wide lenticular vein in the granodioritic bedrock from the Jurassic. The vein is principally made of hydrothermal pyrolusite, magnetite and goethite which are extremely hard materials and yellow to brown and black. Thus we suppose that the prehispanic miners intensively extracted a peculiar part of the vein, between the hard magnetite and the bedrock, so that few evidence of the material extracted in the mine were recorded. Our investigations focus on the determination of the characteristics of the quite messing materials which were extracted and we try to identify the following phases of transformation and utilisation. In order to address these issues, we sampled and document the lithology of the vein and of the numerous geological formations which provide iron rich materials in the neighbourhood. Furthermore, fragments of iron oxides from the mine refus, as well as red or black residues on tools from divers Archaic sites in the area (hammer stones in the mine, lithic weapons, grinding-stones and shells in the shellmiddens and rock-shelters) in order to compare their mineralogical and geochemical composition. [less ▲]

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See detailNeanderthals see red : production of red powder in the Late Mousterian in Ormesson, France
Salomon, Hélène ULg; Geurten, Stéphanie; Bodu, Pierre et al

Poster (2013, February 07)

Les Bossats, near Ormesson, is a newly discovered late Mousterian site dated around 47.000 B.P. by thermoluminescence. The archaeological level, fossilized by loess, revealed a rich industry based on the ... [more ▼]

Les Bossats, near Ormesson, is a newly discovered late Mousterian site dated around 47.000 B.P. by thermoluminescence. The archaeological level, fossilized by loess, revealed a rich industry based on the discoide mode, associated with numerous fragments of red iron-rich rocks. The geological sources were identified by means of SEM-EDX, XRD, Raman spectroscopy, PIXE and by petrographical observation of thin sections. The past mechanical and morphological modifications of the pigment blocks were characterized by macro-photography, microscopy and topographical micro- measures of the used surfaces. It was thus possible to demonstrate that the colouring materials were selected in the neighbouring by the Neanderthals. Fourteen blocks and fragments show different use wears such as facets, grooves and scars. The Neanderthals implemented numerous techniques in order to produce preferentially red powder. The archaeological remains reveal an organized and versatile processing sequence of red ferruginous materials. During the late Mousterian a great phenomenon in expansion in western Europe is remarkable by the much wider exploitation of mineral red and black materials corresponding to technical modifications and divers utilizations under development. [less ▲]

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See detailPrévalence des cas de lymphadénite granulomateuse sous‐maxillaire chez des porcs abattus en Belgique
Vyt, Philip; Denoël, Joseph ULg; Cassart, Dominique ULg et al

Poster (2013, February 06)

In pigs the Mycobacterium avium complex (MAC) causes granulomatous lymphadenitis. Carcasses with such lesions must be detected, as parts of the affected carcasses and organs have to be condemned. These ... [more ▼]

In pigs the Mycobacterium avium complex (MAC) causes granulomatous lymphadenitis. Carcasses with such lesions must be detected, as parts of the affected carcasses and organs have to be condemned. These nontuberculous mycobacteria are opportunistic pathogens which have acquired an increasing importance in public health in recent decades due to their ability to cause lung diseases, lymphadenitis in children and systemic infections in immunocompromised patients ‐ even if the potential risk of infection of an immunocompromised person by MAC in the consumption of undercooked pork still has to be determined. The first objective of this study was to evaluate the prevalence of submandibular granulomatous lymphadenitis in pigs slaughtered in Belgium. Between August 2010 and September 2011, 16,211 carcasses were inspected by the same veterinarian in 2 slaughterhouses – one in Flanders, the other in Wallonia. Eighty‐six suspected cases of submandibular granulomatous lymphadenitis (0.53% of pigs; 95% Confidence Interval: 0.42‐0.65%) were identified, collected and submitted to histopathological (Ziehl‐Neelsen and haematoxylin‐eosin staining) and bacteriological (culture, PCR, molecular typing) tests. The second objective of the study was to characterize lesions and to identify the relative importance of MAC and Rhodococcus equi to explain the lesions. Mycobacterium avium subsp. hominissuis (MAH) was isolated from 6 lymph nodes (7.0%; 95% CI: 2.6‐14.6%) and Rhodococcus equi from 45 (52.3%; 95% CI: 41.3‐63.2%). The final objective of the study consisted in farm investigation to evaluate the possible source of contamination of pigs by MAH. Potential sources such as sawdust, water, wild birds and/or cattle were identified. [less ▲]

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See detailComparaison of the allergenicity of the zymogen and mature form of Der p 3.
Bouaziz, Ahlem ULg

Poster (2013, February)

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See detailModeling the mutation of socio-ecosystems in Central Africa
Gillet, Pauline ULg; Vermeulen, Cédric ULg

Poster (2013, February)

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See detailScan for selective sweeps associated with muscular devolpment in Belgian Blue beef cattle
Druet, Tom ULg; Ahariz, Naïma ULg; Cambisano, Nadine ULg et al

Poster (2013, February)

The Belgian Blue beef cattle is well known for its double muscling phenotype resulting from fixation of a deletion in the myostatin gene. Since this fixation, further intensive selection for muscular ... [more ▼]

The Belgian Blue beef cattle is well known for its double muscling phenotype resulting from fixation of a deletion in the myostatin gene. Since this fixation, further intensive selection for muscular development has been particularly succesful. This response to selection might be due to fixation of more genetic variants increasing muscular development. In the present study, we search for selective sweeps in the Belgian Blue genome which might result from the fixation of such variants. To that end we used data from 593 sires genotyped with the BovineHD SNP array. In addition, we used the Belgian Blue dual purpose and the Holstein breeds as controls. We first performed scans for regions of complete homozygosity resulting from fixation. Large fixed regions were found around major genes known to be fixed in the Belgian Blue cattle breed (MSTN, PLAG1 and MC1R) but no other regions of the same magnitude was found. Among the smaller identified regions, only few of them were unique to the Belgian Blue breed. Statistical tests based on long range haplotype information were also implemented, revealing large regions in the genome of reduced haplotype homozygosity specific to the Belgian Blue breed. Some of these regions corresponded to known major genes (MSTN, roan locus, PLAG1 or MC1R) while other regions were new. To determine whether these regions might be the result of selection for muscular development, we performed association studies for muscular development. None of the identified QTL matched with the regions of reduced haplotype homozygosity and the largest QTLs did not presented evidence of strong selective sweeps. These results suggest that the response to selection for muscular development in Belgian Blue beef cattle is probably the result of polygenic selection. [less ▲]

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See detailPrevalentie van eenzaamheid bij 65-plussers in België
Vermeulen, Bram; Maggi, Patrick ULg; Delye, Samuel ULg et al

Poster (2013, February)

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See detailUnravelling the roles of lysine acetylation by Elp3 during inner ear development
Mateo Sanchez, Susana ULg; Delacroix, Laurence ULg; Laguesse, Sophie ULg et al

Poster (2013, January 28)

The inner ear is composed of the vestibular system that controls balance, and the cochlea, which is dedicated to hearing. In both parts of the inner ear, sensory epithelia comprise supporting cells ... [more ▼]

The inner ear is composed of the vestibular system that controls balance, and the cochlea, which is dedicated to hearing. In both parts of the inner ear, sensory epithelia comprise supporting cells surrounding the sensory hair cells. These cells bear at their apical surface a staircase-structured bundle, consisting of multiple rows of actin-based stereocilia and a single tubulin-based kinocilium. This hair bundle allows the transduction from mechanical stimuli, initiated by sound or gravitational changes, to electrical signals that will then be transmitted by neurons from the spiral ganglion (innervating hair cells of the cochlea) or the vestibular ganglion. The inner ear organogenesis requires a tightly regulated transcriptional program that can be affected by post-transcriptional and post-translational modifications among which lysine acetylation. Given the importance of acetylation homeostasis in controlling developmental processes, we planned to investigate its role in inner ear formation and focused our attention on Elp3 acetyl-transferase, a member of the Elongator complex recently implicated in neurogenesis. To determine the role of Elp3 in the inner ear, we first determine the spatio-temporal pattern of ELp3 mRNA expression and showed that it was expressed in the entire early otocyst at E11.5 and persisted later in the sensory epithelium of the cochlea (the organ of Corti), in the spiral ganglion, in the stria vascularis and in the vestibule. To unravel in vivo functions of Elp3 in the inner ear, we have generated conditional knock-out mice (Elp3 cKO). We submitted these mice to a battery of vestibular testing (i.e. stereotyped circling ambulation, head bobbing, retropulsion, and absence of reaching response in the tail-hanging test) and found significant abnormalities. Besides, compared to wild-type mice, the auditory brain stem response of Elp3 cKO indicated that these mice are severely deaf. At the cellular level, we did not found any structural abnormalities nor cell patterning impairments that could explain deafness or balance dysfunction in Elp3 cKO mice. However, we detected some defaults in the planar orientation of their auditory hair cell bundle. In addition, the length of the kinocilium was significantly reduced both in vestibular and cochlear hair cells from Elp3 cKO mice compared with wild type littermates. We were also able to demonstrate an increased level of apoptosis in the Elp3 cKO spiral ganglion at E14.5 leading to a reduced number of fibers innervating the cochlear hair cells as well as a reduced number of their synaptic ribbons at P0 and P15. In conclusion, our results clearly showed a role of Elp3 both in hearing and balance. We plan to go deeper in the mechanisms involved through the identification of the proteins acetylated by Elp3. [less ▲]

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See detailRole of Gamma Delta T cells in HPV-induced Cancer Progression
Van hede, Dorien ULg; Bastin, Renaud; Francis, Floriane et al

Poster (2013, January 28)

Detailed reference viewed: 44 (12 ULg)
See detailChemical probes and signaling pathways for the orphan GPCR GPR27
Dupuis, Nadine ULg; Gilissen, Julie ULg; Pirotte, Bernard ULg et al

Poster (2013, January 28)

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See detailDifferentiation of Boettcher's Cells During Postnatal Development of Rat Cochlea
Cloes, Marie ULg; Renson, Thomas; Johnen, Nicolas ULg et al

Poster (2013, January 28)

Detailed reference viewed: 14 (7 ULg)
See detailInteractomic map of the Ets factors family : Identification of unexpected functions in mRNA processing
Rambout, Xavier ULg; Simonis, Nicolas; Brohée, Sylvain et al

Poster (2013, January 28)

The Ets factors are a family of 27 transcription factors characterized by their unique DNA-binding domain. We aimed at building a protein-protein interaction (PPI) map (interactome) of the human Ets ... [more ▼]

The Ets factors are a family of 27 transcription factors characterized by their unique DNA-binding domain. We aimed at building a protein-protein interaction (PPI) map (interactome) of the human Ets factors in order to better define their roles and regulations in normal and oncogenic processes. The Ets interactome was built on a high-throughput yeast-two hybrid (Y2H) approach, and a literature and database curation. We identified 431 PPIs and 276 different protein partners. Clustering of the Ets interactome divided it into 24 functional subnetworks classified on their novelty index and their size. Cluster#1 was exclusively composed of newly identified interaction partners and was highly connected to the Erg subfamily of Ets factors. Gene ontology enrichment analysis revealed that it was associated to mRNA processing. In support of this result, we observed in HeLa cells that ERG and the components of cluster#1 localized in p-bodies and stress granules, physically linked cytoplasmic sites of mRNA degradation and silencing. Hence, we hypothesized that Erg proteins might have a role in post-transcriptional gene regulation and be involved in cellular mRNAs degradation. To test this hypothesis, we performed a MS2-based tethering assay and showed that the recruitment of ERG on a mRNA reporter promoted inhibition of its expression via a two-fold decrease of its half-life. ERG controls degradation of target mRNAs via different mechanisms including polysome stability, mRNA deadenylation, and p-bodies aggregation. A microarray-based appraoch identified 321 endogeneous genes whose mRNA decay rate was lowered in ERG silenced cells. Results point out the Nter domain of ERG as the predominant domain required for mRNA degradation. Importantly, oncogenic TET-Erg fusions described in AML and Ewing’s sarcoma exhibited diminished ability to degrade target mRNAs, concomitantly with the loss of the ERG Nter domain. This reinforces the important role of Erg proteins in mRNA degradation in cancer. [less ▲]

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See detailDifferentiation of Boettcher’s Cells during Postnatal Development of Rat Cochlea
Cloes, Marie; Renson, Thomas; Johnen, Nicolas et al

Poster (2013, January 28)

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See detailWhole organ culture in rotating bioreactor: the rat embryonic inner ear
Renauld, Justine ULg; Johnen, Nicolas ULg; Hubert, Pascale ULg et al

Poster (2013, January 28)

In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is ... [more ▼]

In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is composed by two types of cells: sensory hair cells and non-sensory supporting cells. All these cells are distributed according to a specific arrangement along the whole length of the cochlea. So far, the mammalian inner ear is very sensitive to damage, with no hair cell replacement or cell proliferation occurring in the cochlea. That is why understanding the mechanisms that regulate the mammalian cochlear development is important for pursuing strategies to induce sensory hair cells regeneration. Here, we present a technique of whole embryonic inner ear culture in rotating bioreactors. Besides, we compare two different culture media, DMEM and Neurobasal-A. Rat inner ears are sampled at the 16th embryonic day (E16) and grown in rotating bioreactors during 48h or six days. After 48h, semithin sections realized in the growing cochlea show the development of the ventral epithelium and ultrathin sections confirm the differentiation of the sensory hair cells. Using immunochemistry techniques on our material after 48h or six days in vitro, we show that all the cells of the organ of Corti are differentiating, whichever the culture medium used. Our preliminary results demonstrate that organ culture of the embryonic inner ear in rotating bioreactor is possible. Such a method provides an in vitro model for the investigation of developmental, regulatory, and differentiation processes that could be helpful in the understanding of the mechanisms underlying the development of the mammalian cochlea. [less ▲]

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See detailCis-acting inhibition of MHC class I-restricted epitope presentation by Alcelaphine herpesvirus 1 genome maintenance protein
Sorel, Océane ULg; Myster, Françoise ULg; Vanderplasschen, Alain ULg et al

Poster (2013, January 18)

γ-Herpesviruses persist as latent episomes in actively dividing lymphocytes. Their consequent need to express a viral genome maintenance protein (GMP) during latency presents a potential immune target ... [more ▼]

γ-Herpesviruses persist as latent episomes in actively dividing lymphocytes. Their consequent need to express a viral genome maintenance protein (GMP) during latency presents a potential immune target. However, the GMPs from several γ-herpesviruses have evolved related strategies to limit their own MHC class I epitope presentation to cytotoxic T lymphocytes (CTLs). Alcelaphine herpesvirus 1 (AlHV-1) is a γ-herpesvirus that persists asymptomatically in its natural host, the wildebeest. However, AlHV-1 transmission to a large number of susceptible ruminants, including cattle, results in the development of a lethal lymphoproliferative disease named malignant catarrhal fever (MCF). We recently observed that the AlHV-1 GMP-homologue encoded by ORF73 is highly expressed during MCF and that the impairment of its expression renders AlHV-1 unable to induce MCF. With its 1300 aa, AlHV-1 ORF73 is the largest γ-herpesvirus GMP described to date and contains a large acidic internal repeat region that could be involved in the cis-acting CTL evasion mechanism. Here, we sought to determine the CTL evasion properties of AlHV-1 ORF73. We first performed bioinformatic analyses to characterize the protein domains. Then, we used an in vitro assay to demonstrate that ORF73 severely limits the presentation at the cell surface of an MHC class I-restricted epitope linked to ORF73 in cis. These results suggest that AlHV-1 has developed mechanisms to evade cytotoxic anti-viral response during latency. The exact mechanisms explaining the presentation defect remain to be deciphered as well as the role of the cis-acting CTL evasion mechanism of ORF73 in the pathogenesis of MCF. [less ▲]

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See detailI am (un)happy but I don't know why: Subliminal positive-self statements effects
Bustin, Gaëlle ULg; Weinberger, Joel

Poster (2013, January)

This study provides evidence that positive self-statements can increase mood among unhappy people if they are presented subliminally. In study 1, participants with low and high levels of dispositional ... [more ▼]

This study provides evidence that positive self-statements can increase mood among unhappy people if they are presented subliminally. In study 1, participants with low and high levels of dispositional happiness were presented subliminally with the two words I AM which were immediately paired with a positive word. Results revealed that being exposed to subliminal positive self-statements seems to provide a boost in mood for people with less happy dispositions. Surprisingly, opposite effects were found for participants who had joyful dispositions: exposure to subliminal positive self-statements tended to lower their mood. Study 2 confirmed these results with an implicit measure of mood. Such results suggest that subliminal messages can affect emotions and highlight the necessity of taking personality into account in unconscious cognition research. [less ▲]

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See detailValidation of a French Version of a New Anxiety Trait Scale for Children
Geurten, Marie ULg; Meulemans, Thierry ULg; Fresson, Megan ULg et al

Poster (2013)

Anxiety diagnosis is relatively complex in children because intensity as well as symptoms of anxiety change during childhood (Bouden, Halayem, & Fakhfakh, 2002). The principal aim of this study was to ... [more ▼]

Anxiety diagnosis is relatively complex in children because intensity as well as symptoms of anxiety change during childhood (Bouden, Halayem, & Fakhfakh, 2002). The principal aim of this study was to validate through Confirmatory Factor Analyses (CFA) the a priori three-factor structure of the French version of the anxiety trait scale for children which includes psychological (“tend to be worried about everything”), behavioral (“tend to be upset, nervous or grumbling”), and somatic symptoms (“headache complaints”) of anxiety. This scale was previously found to discriminate, with high sensitivity and specificity, children with anxiety from control group. A first CFA performed on 288 6-12 year-old children showed an acceptable fit (2/df =2.66; RMSEA=.07 and CFI=.94). A second three-factor model was constructed and showed a better fit with a new sample of 287 children (2/df =2.18; RMSEA=.06 and CFI=.96), with a lower ECVI value for the model 2. For this model, the Cronbach’s alpha for each of the subscales ranged from .71 to .86, which confirmed the good internal reliability of the scale. This study provides a new three-factor structure for this anxiety scale and proposes normative data for French-speaking children. [less ▲]

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See detailL’agriculture périurbaine de la ville de Kinshasa et les revenus des maraîchers
Masiala Bode, Mabu; Lebailly, Philippe ULg

Poster (2013)

Ce poster vise à étudier la dynamique spatiale du site de Nzeza Nlandu, à déterminer les revenus tirés du maraîchage, à analyser le lien qui existe entre les revenus maraîchers et les superficies ... [more ▼]

Ce poster vise à étudier la dynamique spatiale du site de Nzeza Nlandu, à déterminer les revenus tirés du maraîchage, à analyser le lien qui existe entre les revenus maraîchers et les superficies emblavées et, à faire ressortir la contribution des revenus maraîchers dans les dépenses courantes des ménages exploitants. [less ▲]

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See detailEffects of diazoxide, benzothiadiazine and benzopyrane derivatives on mitochondrial proton and electron leaks of cardiomyocytes (H9C2 cell line).
Mouithys-Mickalad, Ange ULg; Ceusters, Justine ULg; Charef, M et al

Poster (2013)

Background: Mitochondria are double membrane- organelles that play a central role in cellular metabolism, calcium homeostasis and redox signaling. They have been also considered as main producers of ... [more ▼]

Background: Mitochondria are double membrane- organelles that play a central role in cellular metabolism, calcium homeostasis and redox signaling. They have been also considered as main producers of adenosine triphosphate (ATP) and reactive oxygen species (ROS). In many cancer cells those organelles become dysfunctional leading to a shift of energy metabolism from oxidative phosphorylation to active glycolysis and an increase of ROS generation. According to Warberg’ theory, cancer damage might occur at the mitochondrial level, affecting tiny structures within each cell implicated in the energy production through ATP. New insight is that mitochondria might be a good therapeutic target for metabolic syndromes, ischemia/reperfusion injury and organs transplantation. Therefore, search for novel molecules able to keep mitochondria functional are of relevant interest. Methodology: Cardiomyocytes (H9C2 cells) were from ATCC (USA) and grown till confluence. The basal cellular respiratory rate, proton and electron leaks as well as ATP production were measured with the High Resolution Oxygraphy (Oroboros, Austria). All compounds: diazoxide (DIAZ), diazoxide –related analogs (1: BPDZ-259, 2: BPDZ-444), and benzopyran derivatives (3: BPDZ-490, 4: BPDZ-711) were tested at final concentration of 10-5 M, except when specified and compared to control samples (cells with or without DMSO). Results and conclusion: The basal respiratory rate of H9C2 cells (5x106/mL) was changed depending on the chemical structure of the tested compounds: e.g. compound 3 strongly enhanced the routine respiration, while 4 displayed a marked lowering effect. In contrast, the addition of similar concentration of benzothiadiazin derivatives (1, 2) had no effect on routine respiration but also on the other respiratory parameters such as oligomycin-induced leak and ATP production. Similar profile was obtained with the reference molecule: diazoxide. Overall, our findings indicate that both diazoxide-like analogues (1 and 2) and diazoxide were without significant effect on basal respiration, ATP production, even on maximal respiration. Interestingly, two derivatives show opposite effects: compound 3 behaves as a uncoupling agent and the other one (4) exhibits a real lowering effect on respiration but that was reversible. The latter effect might be of interest if this kind of molecules could be used for further use as an agent for organ conservation during transplantation. Our results also demonstrate that diazoxide, a well-known Mito-KATP opener, did not exert its effect beside of clinical situation like ischemia/reperfusion injury. [less ▲]

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See detailNear-infrared spectroscopy for analysis of oil content and fatty acid profile in Jatropha curcas L. flour
Terren, Marieke ULg; Lecler, B.; Baeten, V. et al

Poster (2013)

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See detailThermal and structural behaviour of four industrial laurie fats
Anihouvi, Prudent; Blecker, Christophe ULg; Dombrée, Anne et al

Poster (2013)

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See detailExpression of a protease of biotechnological interest cloned from C. d. collilineatus venom gland
Boldrini-Franca, Johaha; Rodrigues, RS; Santos-Silva, LK et al

Poster (2013)

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See detailCurrent net ecosystem exchange of CO2 in a young mixed forest: any heritage from the previous ecosystem?
Violette, Aurélie ULg; Heinesch, Bernard ULg; Erpicum, Michel ULg et al

Poster (2013)

For 15 years, networks of flux towers have been developed to determine accurate carbon balance with the eddy-covariance method and determine if forests are sink or source of carbon. However, for ... [more ▼]

For 15 years, networks of flux towers have been developed to determine accurate carbon balance with the eddy-covariance method and determine if forests are sink or source of carbon. However, for prediction of the evolution of carbon cycle and climate, major uncertainties remain on the ecosystem respiration (Reco, which includes the respiration of above ground part of trees, roots respiration and mineralization of the soil organic matter), the gross primary productivity (GPP) and their difference, the net ecosystem exchange (NEE) of forests. These uncertainties are consequences of spatial and inter-annual variability, driven by previous and current climatic conditions, as well as by the particular history of the site (management, diseases, etc.). In this study we focus on the carbon cycle in two mixed forests in the Belgian Ardennes. The first site, Vielsalm, is a mature stand mostly composed of beeches (Fagus sylvatica) and douglas fir (Pseudotsuga menziesii) from 80 to 100 years old. The second site, La Robinette, was covered before 1995 with spruces. After an important windfall and a clear cutting, the site was replanted, between 1995 and 2000, with spruces (Piceas abies) and deciduous species (mostly Betula pendula, Aulnus glutinosa and Salix aurita). The challenge here is to highlight how initial conditions can influence the current behavior of the carbon cycle in a growing stand compared to a mature one, where initial conditions are supposed to be forgotten. A modeling approach suits particularly well for sensitivity tests and estimation of the temporal lag between an event and the ecosystem response. We use the forest ecosystem model ASPECTS (Rasse et al., Ecological Modelling 141, 35-52, 2001). This model predicts long-term forest growth by calculating, over time, hourly NEE. It was developed and already validated on the Vielsalm forest. Modelling results are confronted to eddy-covariance data on both sites from 2006 to 2011. The main difference between both sites seems to rely on soil respiration, which is probably partly a heritage of the previous ecosystem at the young forest site. [less ▲]

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See detailTypologie des exploitations agricoles familiales du territoire de Kipushi/RDC
Tshomba Kalumbu, John; Nkulu Mwine Fyama, Jules; Berti, Fabio ULg et al

Poster (2013)

Ce poster vise à établir la typologie structurelle et fonctionnelle des exploitations agricoles familiales du territoire de Kipushi et à identifier et analyser les facteurs de production susceptibles ... [more ▼]

Ce poster vise à établir la typologie structurelle et fonctionnelle des exploitations agricoles familiales du territoire de Kipushi et à identifier et analyser les facteurs de production susceptibles d’influencer le niveau de compétitivité de cette agriculture périurbaine. [less ▲]

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See detailAvantages et inconvénients d’une forêt communautaire
Meunier, Quentin; Boldrini, Sylvie; Boukouendji, B. et al

Poster (2013)

Le poster présente de façon didactique les avantages et inconvénients pour une communauté villageoise de démarrer un processus de forêt communautaire au Gabon

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See detailPreserved automatic inhibition effect after 1 Hz repetitive transcranial magnetic stimulation over the supplementary motor area
D'Ostilio, Kevin ULg; CREMERS, Julien ULg; DELVAUX, Valérie ULg et al

Poster (2013)

Background: It is widely accepted that medial frontal regions are involved in voluntary action control. Indeed, Sumner et al. (2007) have recently suggested that one of the mechanisms through which the ... [more ▼]

Background: It is widely accepted that medial frontal regions are involved in voluntary action control. Indeed, Sumner et al. (2007) have recently suggested that one of the mechanisms through which the supplementary motor area (SMA) contributes to voluntary control is automatic and unconscious motor inhibition. In this study, they administered a visuo-motor subliminal masked prime task (Eimer & Schlaghecken, 2003) to two patients with micro-lesions of the SMA and demonstrated an absence of automatic and unconscious inhibition as evoked by masked prime stimuli. This finding has been supported by neuroimaging data (D'Ostilio et al., 2012). Here, the aim of our research was to corroborate this result by means of a “virtual lesion” approach. Methods: For this purpose, we examined the effects of 1 Hz rTMS (train of 20 min; stimulus intensity 120 % of resting motor threshold) over the SMA of ten healthy volunteers, previously localized by functional magnetic resonance imaging (fMRI), on reaction time (RT) performance in the subliminal masked prime task. The functional localizer experiment consisted of four blocks of sequential finger tapping and 15 s of rest after each block. Imaging data were analyzed with SPM 8 and then were imported into the Brainsight software version 2.1.5. With such system, we were able to navigate across the subjects’ brain. The peak voxel in the SMA for each subject (at a statistical threshold of p < 0.05 uncorrected) was used as a target point for the rTMS session. Results: The mean motor threshold was 50.9 % of maximal stimulator output (SD: ± 4.86 %). Wilcoxon tests showed a significant effect of compatibility on RTs (sham: Z = 2.7, p = 0.007; rTMS: Z = 2.8, p = 0.005) and accuracy rate (sham: Z = 2.5, p = 0.01; rTMS: Z = 2.1, p = 0.03), subjects being slower and making more errors in compatible trials (sham: 391.64 ± 52 ms, 87.3 % of accuracy; rTMS: 396.66 ± 37 ms, 86.3 % of accuracy) in comparison to incompatible trials (sham: 357.45 ± 36 ms, 92.5 % of accuracy; rTMS: 356.25 ± 28 ms, 92.7 % of accuracy), suggesting motor inhibition. However, this NCE was preserved after rTMS over the SMA (RTs: Z = 0.87, p = 0.39; accuracy rate: Z = 0.71, p = 0.47). Conclusions: We conclude that long trains of low intensity 1 Hz rTMS did not affect the modulation of RT by subliminal stimuli, suggesting that the SMA might not be mandatory for the implementation of this automatic process. The limitation of this study is relative to the neural efficacy argument because we are not sure that TMS was strong enough to disturb the redundant organizational processing in the SMA or that other regions were not able to compensate for the virtually lesioned area. [less ▲]

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See detailCharacterization of olefins from enzymatically interesterified palm oil
Gibon, Véronique; Maes, Jeroen; Dijckmans, Peggy et al

Poster (2013)

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See detailEffect of humic substances on in vitro rooting of alder (Alnus glutinosa (L.) Gaertn) and birch (Betula pendula Roth)
Tahiri, Abdelghani ULg; Destain, Jacqueline ULg; Druart, Philippe et al

Poster (2013)

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic ... [more ▼]

Humic substances (HS) are organic compounds resulting from the physical, chemical and microbiological transformations of organic residues. Present everywhere in the nature; they are taking part in basic functionalities in any ecosystems involving soils, sediments, water and landfills. They are heterogeneous and complex carbon macromolecules. Our study aims to compare the effect of HS from différent sources on in vitro rooting of woody plants. [less ▲]

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See detailEnzymatic remediation of crude palm oil
Gibon, Véronique; Kodali, Sitharam; Maes, Jeroen et al

Poster (2013)

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See detailMulti-Scale Modelisation of the Optical Second Harmonic Generation of Tyrosine-Containing Iturinic Antimicrobial Lipopeptides
Loison, Claire; Nasir, Mehmet Nail ULg; Benichou, Emmanuel et al

Poster (2013)

The optical Second Harmonic Generation (SHG) of Mycosubtilin Langmuir Layers at the air/water interface are modeled. In this tyrosine-containing lipopepeptide, the environment of the tyrosine residue is ... [more ▼]

The optical Second Harmonic Generation (SHG) of Mycosubtilin Langmuir Layers at the air/water interface are modeled. In this tyrosine-containing lipopepeptide, the environment of the tyrosine residue is critical in defining the SHG response. To propose reasonable the structure of Mycosubtilin aggregates at the air-water interface, molecular dynamics simulations (all-atom and coarse-grained) are performed. Molecular hyperpolarizability of the lipopeptide are obtained by Quantum Chemistry calculations. Finally, the SHG susceptibilities of the interface are calculated using a simple additive model. The molecular dynamics simulations suggest that lipopeptides aggregate at the interface into half-micelles, and that this phenomena is the origin of a constraint on Tyrosine orientation. In particular the C-OH bond of the Tyrosine residue has a preferential orientation along the interface normal, pointing towards the air. This inhomogneneous orientation distribution, associated with a dominant hyperpolarizability component along the C-OH bond of the Tyrosine, yields a characteristic SHG response. Comparison with experimental data gathered in our lab are proposed. [less ▲]

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See detailEstimating Inter-Sensor Sea Surface Temperature Biases using DINEOF analysis
Tomazic, Igor ULg; Alvera Azcarate, Aïda ULg; Troupin, Charles ULg et al

Poster (2013)

Climate studies need long-term data sets of homogeneous quality, in order to discern trends from other physical signals present in the data and to minimise the contamination of these trends by errors in ... [more ▼]

Climate studies need long-term data sets of homogeneous quality, in order to discern trends from other physical signals present in the data and to minimise the contamination of these trends by errors in the source data. Sea surface temperature (SST), defined as one of essential climatology variables, has been increasingly used in both oceanographical and meteorological operational context where there is a constant need for more accurate measurements. Satellite-derived SST provides an indispensable dataset, with both spatially and temporally high resolutions. However, these data have errors of 0.5 K on a global scale and present inter-sensor and inter-regional differences due to their technical characteristics, algorithm limitations and the changing physical properties of the measured environments. These inter-sensor differences should be taken into account in any research involving more than one sensor (SST analysis, long term climate research . . . ). The error correction for each SST sensor is usually calculated as a difference between the SST data derived from referent sensor (e.g. ENVISAT/AATSR) and from the other sensors (SEVIRI, AVHRR, MODIS). However, these empirical difference (bias) fields show gaps due to the satellite characteristics (e.g. narrow swath in case of AATSR) and to the presence of clouds or other atmospheric contaminations. We present a methodology based on DINEOF (Data INterpolation Empirical Orthogonal Functions) to reconstruct and analyse SST biases with the aim of studying temporal and spatial variability of the SST bias fields both at a large scale (European seas) and at a regional scale (Mediterranean Sea) and to perform the necessary corrections to the original SST fields. Two different approaches were taken: by analysing SST biases based on reconstructed SST differences and based on differences of reconstructed SST fields. Corrected SST fields based on both approaches were validated against independent in situ buoy SST data or with ENVISAT/AATSR SST data for areas without in situ data (e.g. eastern Mediterranean). [less ▲]

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See detailSelf-coverage of Leidenfrost droplets
Maquet, Laurent ULg; colinet, pierre; Moreau, Florian ULg et al

Poster (2013)

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See detailThe adventures of a little star
Nazé, Yaël ULg; Thonnard, Florine

Poster (2013)

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See detailEvaluation of a new [18F] labeled tracer targeting synaptic vesicle protein 2C by ex vivo autoradiography and in vivo PET study in rat brain.
Warnock, Geoffrey; Aerts, Joël ULg; Mestdagh, Nathalie et al

Poster (2013)

Introduction The synaptic vesicle protein 2 (SV2) family is a group of integral membrane glycoproteins homologous to the major facilitator superfamily and could be involved in several neuronal diseasesa ... [more ▼]

Introduction The synaptic vesicle protein 2 (SV2) family is a group of integral membrane glycoproteins homologous to the major facilitator superfamily and could be involved in several neuronal diseasesa. The binding of the novel, no-carrier-added, [18F] labeled compound [18F]UCB-F to the SV2C isoform was evaluated in rat brain. Methods Radiochemistry No-carrier added [18F]UCB-F was obtained following the method shown in Fig. 1. The identity and purity of the tracer were evaluated by radioUPLC and chiral radioHPLC. Autoradiography Sprague Dawley rat brain sections were incubated at RT with buffered [18F]UCB-F solutions and exposed on film. Matching sections were stained with cresyl violet for structural identification. PET studies PET studies (Siemens Concorde Focus 120 µPET) were performed under isoflurane anesthesia. The tracer was injected as a bolus via the tail vein. After a 10-min transmission scan to correct for attenuation, dynamic emission data was recorded for a total of 60 min. The impact of P-glycoprotein (P-gp) activity on tracer uptake in the brain was evaluated using cyclosporine (50 mg/kg SC). Metabolite analysis During PET studies, arterial blood samples were taken for the measurement of tracer metabolites. Plasma was separated by centrifugation and proteins were acid-precipitated. Metabolites were detected using HPLC and confirmed by gamma counting. Results The tracer was obtained with a decay corrected yield of ±10%. Specific activity ranged from 10 GBq/µmol to 40 GBq/µmol. Ex vivo autoradiography showed that the binding of [18F]UCB-F to SV2C closely matched the expected distribution b (Fig.2). In vivo PET studies revealed that [18F]UCB-F briefly entered the brain, but exhibited extremely rapid washout. A large accumulation in the liver and intestines was observed. Metabolite analysis in the plasma revealed high protein binding and rapid metabolism. Inhibition of P-gp transport with cyclosporin had no clear effect on the rapid washout from the brain. Conclusions Despite a close match between [18F]UCB-F SV2C binding and the expected brain distribution, the pharmacokinetics in rat brain appear unfavorable for the use of this tracer to quantify SV2C in vivo. Acknowledgement / References a Lynch & al (2004) Proc. Natl. Acad. Sci. USA 101:9861 b Janz & Sudhof (1999) Neuroscience 94:1279 c The authors thank the Walloon Region and the FRNS Belgium for financial support. [less ▲]

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See detailExploring the sky
Nazé, Yaël ULg

Poster (2013)

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See detailAltered mitochondrial oxidative phosphorylation capacity in horses suffering from polysaccharide storage myopathy
Tosi, Irène ULg; Art, Tatiana ULg; Cassart, Dominique ULg et al

Poster (2013)

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is ... [more ▼]

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is characterized by an accumulation of abnormal glycogen in myofibers due to a genetic defect in the skeletal muscle glycogen synthase (GYS1) enzyme. We hypothesized that the energetic production through the oxidative phosphorylation (OXPHOS) in muscular mitochondria might be impaired in type-1 PSSM-affected horses. Material and Methods: Eight horses with a history of exertional rhabdomyolysis were tested for the GYS1 mutation. Muscle biopsies were collected and used for histological analysis and high resolution respirometry (HRR). HRR values from 3 groups of horses (PSSM-positive horses, horses with a history of myopathy but negative to PSSM and healthy controls) were compared using a linear mixed model to take into account repeated (2-3 times) measurements made for each horse. Results: In four horses histology revealed an accumulation of abnormal glycogen in myofibers. A severe depression of the maximal OXPHOS capacity was observed by HRR in all horses with exertional rhabodmyolysis, with lower values in PSSM-positive cases. Conclusions: Our study shows a severe decreased OXPHOS capacity in PSSM-affected horses. PSSM is considered primarily a defect in glycogen synthesis but altered OXPHOS might play a central role in its pathogenesis. [less ▲]

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See detailConstaining the wind collision region geometry in the WN+O binary V444 Cyg
Lomax, Jamie; Nazé, Yaël ULg; Hoffman, jennifer

Poster (2013)

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See detailNovel cooperation between CX3CL1 and CCL26 inducing NK cell chemotaxis via CX3CR1: a possible mechanism for NK cell infiltration of the allergic nasal tissue
EL SHAZLY, Amr ULg; Castillo-Doloriert, Hugo; Bisig, Bettina et al

Poster (2013)

Background: Recent data indicated that natural killer (NK) cells and chemokines could play a pivotal role in nasal inflammation. CX3CR1, the only receptor for fractalkine/ CX3CL1, is abundantly expressed ... [more ▼]

Background: Recent data indicated that natural killer (NK) cells and chemokines could play a pivotal role in nasal inflammation. CX3CR1, the only receptor for fractalkine/ CX3CL1, is abundantly expressed by NK cells, and was recently shown to also be a receptor for eotaxin-3/CCL26. However, no reports explored the NK cells-CX3CL1-CCL26 axis via CX3CR1 in allergy.
Objective: Our goals were first to determine specifically NK cell recruitment pattern in nasal tissue of allergic chronic rhinosinusitis (ACRS) and non-allergic chronic rhinosinusitis (NACRS) patients in comparison with healthy controls, and secondly, to investigate the function of CX3CR1 in NK cell migration. Methods: Immunohistochemistry, microchemotaxis chambers, flow cytometry and confocal microscopy were used in this study. Results: Herein, we showed that NK cells infiltrated the epithelial layers of nasal tissue only in ACRS patients and not in NACRS patients or controls. NK cells were also more numerous in the stroma of the nasal tissue from ACRS patients compared with NACRS patients or controls. This migration could be mediated by both CX3CL1 and CCL26, as these two chemokines induced NK cell migration. Moreover, both molecules also stimulated cytoskeleton changes and F-actin reorganisation in NK cells. Chemotaxis and cytoskeleton changes were sensitive to genistein, a tyrosine kinase inhibitor. By flow cytometry, we demonstrated that a single antigen nasal provocation challenge increased the expression of CX3CR1 on NK cells in allergic rhinitis (AR) patients. The function of this receptor was associated with a significant augmentation of NK cell chemotaxis against the optimal doses of CX3CL1 and CCL26. Conclusions and Clinical Relevance: Our results highlight a novel role for CX3CR1 in NK cell migration that may contribute to the NK cell trafficking to the allergic upper airway. This could be mediated largely by CX3CL1 and CCL26 stimulation of the tyrosine kinase pathway. [less ▲]

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