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See detailThe BCCM/ULC collection to conserve the biodiversity and study the secondary metabolites of Polar cyanobacteria
Lara, Yannick ULiege; Durieu, Benoit ULiege; Renard, Marine ULiege et al

Poster (2016, November 16)

In the Polar Regions, Cyanobacteria are the key primary producers and main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in ... [more ▼]

In the Polar Regions, Cyanobacteria are the key primary producers and main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in lakes and soil crusts. Their success in these harsh cold conditions can probably be explained by particular adaptations to survive freeze/thaw cycles, seasonally contrasted light intensities, high UV radiations, dessication and other environmental stresses. The BCCM/ULC public collection is funded by the Belgian Science Policy Office since 2011. It has obtained the ISO9001 certification for deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. This collection aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic mats, soil crusts, cryoconites, endoliths,….) and make it available for researchers to study the taxonomy, evolution, adaptations to harsh environmental conditions, pigments, and genomic make-up. It presently includes 226 cyanobacterial strains, of which 119 are of Antarctic origin (catalogue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). As shown by morphological identification, the strains belong to five orders (Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales). The 16S rRNA and ITS sequences of the strains are being characterized. The first 85 Antarctic strains already studied are distributed into 25 Operational Taxonomic Units (OTUs = groups of sequences with > 97,5% 16S rRNA similarity), and thus, represent a quite large diversity. Moreover, strains identified as members of the genera Leptolyngbya or Phormidium appear in several lineages. This supports the idea that there is a need to revise the taxonomy of these polyphyletic genera with a simple filamentous morphology. To better understand the functioning, metabolism and adaptative strategies of cyanobacteria in the extreme Antarctic environment, the genome sequencing of 11 strains has been started. Pair-read data from illumina MiSeq runs were obtained and submitted to a bioinformatic pipeline dedicated to the assembly of genomes and search of sequences involved in the biosynthesis of secondary metabolites. Gene cluster prediction analysis allowed to characterize 20 clusters of NRPS, PKS and hybrid NRPS-PKS from 2 to 66kb. Surprisingly, none of the characterized operons had previously been described in the literature. [less ▲]

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See detailAnalysis of the interactions of fatty acyds hydroperoxides with biomimetic membranes by complementary biophysical approaches
Deboever, Estelle ULiege; Nasir, Mehmet Nail ULiege; Deleu, Magali ULiege et al

Poster (2016, November 16)

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants ... [more ▼]

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants which are able to induce the natural resistance of plants. Also, they have proved to be excellent candidates for biological control. In this context, the lipoxygenase pathway leads to the formation of fatty acid degradation products, called oxylipins, which appear to be crucial agents in plant defence mechanisms2,3. Moreover, with their broad spectrum of action and their possible inducibility, oxylipins appear to be promising candidates for their use as elicitors4. This work focuses on two hydroperoxy-derived oxylipins, the 13(S)-hydroperoxy-octadecadienoic acid (13-HPOD) and the 13(S)-hydroperoxy-octadecatrienoic acid (13-HPOT). The study of the interaction of such compounds with representative plant plasma membrane lipids is essential to understand plant resistance mechanisms. Several in silico and experimental techniques of biophysics showed that acyl-hydroperoxides have significant adsorption capacity and a strong affinity for model membranes. They may also penetrate biological membrane but no permeabilisation effect was observed in this work. Slight conformational differences seem to have a significant impact on their ability to interact with plant plasma membranes. Based on these results, further investigation of the interactions of fatty acids hydroperoxides, even more on the 9-forms, with plant plasma membranes and eventually in the presence of phytopathogenic species, would allow a better understanding of the innate immunity and, on the longer term, could lead to the development of new elicitors with biological mechanisms potentially independent of membrane protein receptors. [less ▲]

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See detailProtein Factory: A Protein Production and Purification Facility
Bouillenne, Fabrice ULiege; Matton, Anne-Marie ULiege; Thamm, Iris ULiege et al

Poster (2016, November 16)

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See detailA crucial role for cdk1 in postnatal hippocampal neurogenesis
Marlier, Quentin ULiege

Poster (2016, November 15)

Cdk1 seems to be involved in the proliferation and differentiation of adult neural stem cells found in the adult dentate gyrus

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See detailLa promotion des énergies renouvelables : une réponse durable à la problématique énergétique au Tchad
Abdelhamid, Issa Hassane ULiege

Poster (2016, November 14)

La promotion des énergies renouvelables est un thème d’actualité de par le monde. Chaque pays essaie d’apporter des solutions à son bilan énergétique compte tenu de ses paramètres dits de 3E (Énergie ... [more ▼]

La promotion des énergies renouvelables est un thème d’actualité de par le monde. Chaque pays essaie d’apporter des solutions à son bilan énergétique compte tenu de ses paramètres dits de 3E (Énergie-Économie-Environnement). Cette note de recherche privilégie une approche scientifique de l’énergie et du développement durable en l’appliquant au contexte tchadien.Le but assigné à ce poster est de mettre en exergue l'ampleur du patrimoine naturel tchadien en énergies renouvelables (biomasse, éolien, solaire, etc.) en le conciliant avec la nouvelle technologie afin de répondre aux besoins énergétiques des ménages n'ayant pas accès à l'énergie électrique au Tchad... [less ▲]

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See detailDetermination of saccharides in honey using supercritical fluid chromatography coupled with single quadrupole mass spectrometry
Huang, Yang ULiege; Fillet, Marianne ULiege; Zhang, Tingting et al

Poster (2016, November 13)

The efficient separation and accurate determination of saccharides still remains a challenge due to the wide variety of possible isomers, high polarity, similar chemical composition and absence of ... [more ▼]

The efficient separation and accurate determination of saccharides still remains a challenge due to the wide variety of possible isomers, high polarity, similar chemical composition and absence of chromophores [1]. Supercritical fluid chromatography (SFC) is an attractive separation method often showing higher resolution and shorter analysis time compared to traditional GC and HPLC [2]. Few papers have reported on the SFC analysis of saccharides using standard compounds [3-4]. The aim of this study was to develop an efficient and sensitive method for the determination of fructose, glucose and sucrose in honey samples using SFC-MS. The method validation and application to the determination of these saccharides in different honey samples were also performed. [less ▲]

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See detailElektrodynamische Fragmentierung von Elektroschrott
Iseli, Cédric; Streicher-Porte, Martin; Martino, Rémi ULiege et al

Poster (2016, November 11)

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden ... [more ▼]

Untersuchungen dazu werden in diesem Bericht am Beispiel von WEEE und speziell an Leiter-platten aufgezeigt. Die Versuche zeigen, dass EDF für verschiedenste Arten von Elektroschrott eingesetzt werden kann. Verschweisste Kompaktgeräte können zum Beispiel so weit geöffnet werden, dass die ge-wünschte Komponente freigelegt und entfernt werden kann. Leiterplatten haben die Eigenschaft, dass bei kontinuierlich steigendem Energieeintrag zuerst die aufgebrachten Komponenten abgetrennt werden, bevor die Cu-Bahnen freigelegt werden. Bei noch höherem Energieeintrag wird die Leiterplatte schlussendlich zerkleinert und vollständig zer-stört. Durch diese Entfrachtung der Leiterplatte kann ein metallreiches Konzentrat hergestellt werden. [less ▲]

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See detailEndocrine disruptors and parasitism: which effects on the sex behaviour of the amphipod Gammarus pulex ?
Fivet, Adeline; Horion, Sibylle; Thomé, Jean-Pierre ULiege et al

Poster (2016, November 10)

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See detailA Model of Endogenous Insulin Secretion During Exercise
Thomas, Felicity Louise ULiege

Poster (2016, November 10)

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See detailBulk high-temperature superconducting hollow cylinders used for magnetic shielding: effect of an initial trapped field on the shielding performances.
Wera, Laurent ULiege; Fagnard, Jean-François ULiege; Hogan, Kevin ULiege et al

Poster (2016, November 09)

Bulk High-Temperature Superconductors (HTS) can be used as efficient passive magnetic shields with performances exceeding those of conventional ferromagnetic materials. The most common geometry for a ... [more ▼]

Bulk High-Temperature Superconductors (HTS) can be used as efficient passive magnetic shields with performances exceeding those of conventional ferromagnetic materials. The most common geometry for a superconducting shield is a bulk hollow cylinder. In this work, we investigate the magnetic shielding properties when the tube is not in the virgin state. Such a situation happens in practice when the tube has been initially subjected to a first magnetic field. The induced superconducting currents that are flowing in the tube generate a trapped magnetic moment which may deteriorate the shielding of a second field. Here we study experimentally how an initial trapped moment affects the shielding of a magnetic field, when they are both perpendicular to each other. In particular, we focus on the effect of an axial trapped moment on the transverse shielding performances and on the effect of a transverse trapped field on the axial or the transverse shielding performances. Finally, we show how the pristine state of the tube can be restored when applying an external field of adequate amplitude. The consequence for practical applications is that it is possible to recover shielding properties similar to those obtained in the virgin state without heating up the shield above its critical temperature TC. [less ▲]

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See detailDeveloping a hydrodynamical model of the Southern Bight of the North Sea for impact studies
Ivanov, Evgeny ULiege; Capet, Arthur ULiege; Barth, Alexander ULiege et al

Poster (2016, November 08)

In the frame of the Brain FaCE-It project (Functional biodiversity in a Changing sedimentary Environment: Implications for biogeochemistry and food webs in a managerial setting), the impact of fining and ... [more ▼]

In the frame of the Brain FaCE-It project (Functional biodiversity in a Changing sedimentary Environment: Implications for biogeochemistry and food webs in a managerial setting), the impact of fining and hardening resulting from dredging and wind farms installation on the sediment grain size distribution has to be assessed at the scale of the Southern Bight of the North Sea (SBNS) with a particular focus on the Belgian Coastal Zone (BCZ). With this aim, the ROMS-COAWST tri-dimensional (3D) hydrodynamic model is implemented to simulate the hydrodynamics in the SBNS. At its open boundaries with the Atlantic Ocean and the North Sea, the model is forced with the results of a coarse resolution model available from Mercator. A high resolution of 250 m is used in the area of the BCZ where the accuracy of model predictions needs to be refined. Model currents, tides, temperature and salinity fields will be described and first validation exercises with satellite and local data will be presented and discussed in regards with the objectives of FaCE-It. In a next step, the model will be coupled with a sediment transport in order to describe the dynamics of suspended particulate materials (SPM) and the distribution of the seafloor sediment grain size. When finalized the hydrodynamic model will be coupled with a diagenetic model and will provide environmental conditions for scaling up local foodweb studies that are performed in the frame of FaCE-iT. The final aim is to assess the impact of substrate modifications due to aggregate extraction and wind farms on the biogeochemistry, benthic functionality and food webs at local (around the wind farm) and regional scales (SBNS). [less ▲]

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See detailSevere deficiency of the somatotrope GHRH/GH/IGF-1 axis induces a dramatic susceptibility to Streptococcus pneumoniae infection.
Farhat, Khalil ULiege; Bodart, Gwennaëlle ULiege; Desmet, Christophe ULiege et al

Poster (2016, November 07)

Deletion of the growth hormone-releasing hormone gene (Ghrh) results in a severe deficiency of the somatotrope GHRH-GH-IGF-1 axis causing dwarf phenotype that can be reversed by GH or GHRH supplementation ... [more ▼]

Deletion of the growth hormone-releasing hormone gene (Ghrh) results in a severe deficiency of the somatotrope GHRH-GH-IGF-1 axis causing dwarf phenotype that can be reversed by GH or GHRH supplementation (Alba & Salvatori, Endocrinology 2004;145:4134). In basal conditions, the immunological phenotype of Ghrh-/- mice is not markedly disturbed except for a decrease in B cells and an increase in generation of thymic (t) Treg cells (submitted for publication). These data prompted us to investigate immune responses of Ghrh-/- mice using vaccination and infection by S. pneumoniae as models since the response to both stimuli rely on the innate immune system and B cells. Ghrh-/- mice were unable to trigger production of specific IgM and IgG against serotype 1 pneumococcal polysaccharide (PPS) after vaccination with either native PPS (Pnx23) or protein-PPS conjugate (Prev-13) vaccines. A short GH supplementation to Ghrh-/- mice (1 daily injection of 1 mg/kg GH for 4 weeks) restored IgM and IgG response to Pnx23 vaccine but not to Prev-13. This suggests that GH differently impacts on B-1, marginal zone B-2 or innate B-1 B cells. Furthermore, after intranasal instillation of a non-lethal dose of serotype 1 S. pneumoniae, Ghrh-/- mice exhibited a dramatic susceptibility reflected by bacteremia 24h after infection and a survival limit of 72 h, compared to WT C57BL/6 mice that need only 24h to clear infection. The possible impact of GH deficiency on components of the innate immune system that play an important role in defense of the respiratory tract against pneumococcal infection is under current investigation. (*Equal first and last authors. KF is supported by a research grant from the Lebanese Government; GB is Research Assistant, CD is Research Associate, and VG is Research Director at the NFSR of Belgium). [less ▲]

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See detailRisk management in Ivory Coast : Case Study of Population Evictions in Port-Bouët, Abidjan
Comoe, Raymond; Ozer, Pierre ULiege

Poster (2016, November 04)

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See detail« Cap-Haïtien » or « how to ‘construct’ a flood risk in a decade »
Gracius, Gracia Joseph; Ozer, Pierre ULiege

Poster (2016, November 04)

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See detailRecent evolution of the coastline in the Gulf of Guinea. Example of Togo and Benin (2000-2015)
Ozer, Pierre ULiege; Hountondji, Yvon-Carmen; de Longueville, Florence

Poster (2016, November 04)

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See detailPeople moved and will move again
Gemenne, François ULiege; de Longueville, Florence; De Bruyckere, Luka ULiege et al

Poster (2016, November 04)

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See detailImpact of shoreline changes on population in Cotonou, Benin
de Longueville, Florence; Hountondji, Yvon-Carmen; Gemenne, François ULiege et al

Poster (2016, November 04)

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See detailDiagnostic value of serologic tests and seroprevalence of Borreliosis in horses living in southern Belgium
Cerri, Simona ULiege; Meersschaert, C; Houben, Rosa et al

Poster (2016, November 04)

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See detailTerminologia Multilíngue de Clínica General / Medicina de Família e Comunidade
Jamoulle, Marc ULiege; Treuherz, Arthur; Gomes, Luis Filipe et al

Poster (2016, November 02)

Creation of the Q-Codes online knowledge base The list of themes, called Q-Codes has been elaborated in a bottom-up approach, by retrieving, in a qualitative analysis study, the themes most frequently ... [more ▼]

Creation of the Q-Codes online knowledge base The list of themes, called Q-Codes has been elaborated in a bottom-up approach, by retrieving, in a qualitative analysis study, the themes most frequently addressed by GPs when they meet in congresses. A taxonomy has been build. A multilingual terminological record has been developed for each of the 182 items identified. This work is freely available online on www.hetop.eu/Q (free access after inscription) and is supported by the Rouen (France) biomedical informatics department of the University of Rouen (France). All the data are available in the Web Ontology language (OWL-2), an ontology-ready format fit for future use in the semantic web. The product, user guide and e-learning are available on http://3cgp.docpatient.net/ The Q-codes base is aimed at several uses: • Online repository of knowledge specific to GP/FM in several languages • Online PubMed linked bibliographic system easy to use for training in GP/FM • Resource for online e-learning • Resource for analysis of content of congresses in GP/FM in joint usage with ICPC-2 and indexation of gray literature • Research area : o automatic or semi-automatic congresses indexation system o linking GP/FM to the web of data and the linked data initiative The multilingual terminology in GP/FM Out of the online database we are proud to present you the • General Practice / Family Medicine Multilingual Terminology – English version • Terminologia Multilíngue de Clínica Geral / Medicina de Família e Comunidade - Versão em Português • Terminología multilingüe de la medicina general y de familia- Versión española • Terminologie multilingue de médecine générale et de famille – Version française • Een meertalige terminologie in de huisartsgeneeskunde - Nederlandse versie • Từ điển thuật ngữ đa ngôn ngữ về y học tổng quát và y học gia đình – (Vietnamese) • -Codes and definitions will be also available online in Korean and Turkish Those 6 books (60 pages) are readable / downloadable freely online on http://3cgp.docpatient.net/ and contain an explanation of the elaboration process of the taxonomy, a tabular list of the Q-Codes, the list of their definitions and an index. [less ▲]

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See detailSpeech style manipulations in dysarthria in French-speaking children: Acoustic findings.
Ancelle, Josephine; Moya Gale, G.; Hetrick, B et al

Poster (2016, November)

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See detailPreoptic glutamate and estradiol release during male sexual behavior
de Bournonville, Catherine ULiege; de Bournonville, Marie-Pierre ULiege; Aourz, Najat et al

Poster (2016, November)

Beside its long-term control by steroids, male sexual behavior is also modulated by neuroestrogens in a dynamic way (within minutes) in a number of species ranging from fishes to mammals. Studies in male ... [more ▼]

Beside its long-term control by steroids, male sexual behavior is also modulated by neuroestrogens in a dynamic way (within minutes) in a number of species ranging from fishes to mammals. Studies in male Japanese quail have also identified following exposure to a receptive female a rapid decrease in the activity of brain aromatase (AA) the enzyme responsible for the conversion of androgens into estrogens. These effects occur mainly within the medial preoptic nucleus (POM), a sexually dimorphic structure of the preoptic area that plays a key role in the activation of male sexual behavior and contains the highest AA in the brain. In vitro studies demonstrated that AA can be rapidly inhibited by calcium-dependent phosphorylations of the enzyme triggered by the activation of AMPA and kainate receptors. We confirmed here this rapid effect of glutamate on AA by injecting kainate in the POM of anesthetized males and measuring AA in the tissue after brain collection. AA in POM was inhibited in the kainate-injected hemisphere compared to the control hemisphere injected with vehicle. In a second experiment, we showed by in vivo microdialysis that glutamate is released in POM during copulation. These results thus suggest that glutamate controls dynamic changes of AA that occur in the brain during sexual interactions. To confirm that the decrease in AA leads to an actual reduction of local estradiol concentration, we quantified via microdialysis and radioimmunoassay changes in estradiol concentration in the male POM during sexual interactions with a female. Surprisingly, a dramatic elevation of estradiol was observed during copulation. Estradiol has been shown to enhance acutely male sexual motivation, therefore the function of its increase in the POM could be to maintain motivation during the entire sexual encounter. The decrease of AA observed ex vivo after copulation would then reflect a compensatory mechanism to restore baseline pre-copulatory conditions. Importantly, these results highlight that although long-term changes in AA are often used as a proxy for local estradiol concentrations, these two measures can show major short-term discrepancies possibly reflecting variations in estrogen turnover. [less ▲]

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See detailRobustness of spatial autocorrelation tests
Ernst, Marie ULiege

Poster (2016, November)

Spatial autocorrelation expresses the dependence between values at neighbouring locations. Testing if the spatial autocorrelation is significant may confirm or deny the need to consider spatial analysis ... [more ▼]

Spatial autocorrelation expresses the dependence between values at neighbouring locations. Testing if the spatial autocorrelation is significant may confirm or deny the need to consider spatial analysis over the classical one. Indeed, the analysis of spatial data is only meaningful if the spatial components bring information. Several measures of spatial autocorrelation are defined in the literature. Moran’s index, Geary’s ratio and Getis-Ord statistic are the most used statistics. Tests based on these measures have been developed in the literature using asymptotic and permutation results. They are used in practice in many fields, for instance in geography, economics, biogeosciences, medicine, ... However, these tests should be cautiously applied because they are not robust. A single contaminated observation can significantly modify their results. This poster is composed of two parts. Firstly, the robustness of already available tools for measuring spatial autocorrelation will be analysed. Secondly, alternative methods will be proposed to robustly test the spatial autocorrelation. [less ▲]

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See detailGlomerular filtration rate in healthy living potential kidney donors: a meta-analysis.
Pottel, H; Hoste; DELANAYE, Pierre ULiege

Poster (2016, November)

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See detailComparisons of estimated GFR and Cockcroft Gault equations: calibration against relative risk for all-cause mortality.
Warnock, DG; van den Brand, JAJG; DELANAYE, Pierre ULiege et al

Poster (2016, November)

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See detailL’âgisme chez les étudiants en médecine
Corvol, Aline; Missotten, Pierre ULiege; Gentric, Armelle et al

Poster (2016, November)

Introduction: Des stéréotypes négatifs liés à l’avancée en âge sont fréquemment observés en population générale. Des études ont montré que ces stéréotypes sont plus puissants que ceux liés à l’origine ... [more ▼]

Introduction: Des stéréotypes négatifs liés à l’avancée en âge sont fréquemment observés en population générale. Des études ont montré que ces stéréotypes sont plus puissants que ceux liés à l’origine ethnique, au sexe ou à l’orientation sexuelle. Lorsqu’ils sont présents chez les soignants, ces stéréotypes peuvent entrainer des discriminations et limiter l’accès aux soins des personnes âgées (Schroyen et al., 2016). Il a en outre été démontré qu’un comportement d’aide injustifiée pouvait favoriser la perte d’autonomie chez une personne âgée. L’objectif de cette étude est d’évaluer la force des stéréotypes âgistes chez les étudiants en médecine et de rechercher les facteurs qui y sont associés. Méthodes: Un questionnaire écrit informatisé a été proposé aux étudiants en médecine des facultés de Rennes, Brest et Liège, de la 2ème à la 6ème année. Il associe trois mesures : l’Adaptation française de Fraboni Scale of Ageism, une tâche de fluence verbale à propos du vieillissement et de la jeunesse, et une mesure de la stigmatisation liée à l’âge par estimation de pourcentages. Résultats: Des données ont été obtenues pour 570 étudiants à Rennes et 485 à Liège; le recueil de données se poursuit à Brest. Les premières analyses confirment que l’âgisme est largement répandu chez les étudiants en médecine, avec des résultats globalement similaires à Rennes et Liège. Les étudiants de sexe masculins, ceux qui ont peu de contact avec des personnes âgées, ou qui ont des contacts jugés de mauvaise qualité, sont plus susceptibles d’exprimer des stéréotypes âgistes. Le fait d’avoir fait un stage dans un service de gériatrie ou d’avoir bénéficié d’un enseignement de la gériatrie ne semble pas influencer significativement ces stéréotypes. Conclusion: L’enseignement de la gériatrie aux étudiants en médecine doit prendre en compte la fréquence des stéréotypes âgistes. Nos résultats étant soumis à un biais de désirabilité sociale, des études portant sur les stéréotypes implicites (inconscients) seraient utiles. Une meilleure connaissance de l'âgisme et de ses déterminants chez les étudiants en médecine est nécessaire à l'élaboration d'approches pédagogiques susceptibles de limiter les conséquences de ces stéréotypes sur la qualité des soins aux personnes âgées [less ▲]

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See detailThe closure of arteriovenous fistula is associated with a significant acceleration of eGFR decline in kidney transplant recipients
Jouret, François ULiege; DELANAYE, Pierre ULiege; VANDERWECKENE, Pauline ULiege et al

Poster (2016, November)

Background The creation of arteriovenous fistula (AVF) may retard chronic kidney disease progression in the general population. Conversely, there is limited literature regarding the impact of AVF closure ... [more ▼]

Background The creation of arteriovenous fistula (AVF) may retard chronic kidney disease progression in the general population. Conversely, there is limited literature regarding the impact of AVF closure on renal function in kidney transplant recipients (KTR). Methods All KTR were retrospectively identified from 01/01/2007 to 31/12/2013, and grouped into: (0) no AVF; (1) closed AVF; and (2) left open AVF. Glomerular filtration rate was estimated (eGFR) upon MDRD and FAS equations. Linear mixed models calculated the slope and intercept of eGFR decline versus time, starting at 3 months post transplantation (Tx). Comparative analyses of eGFR slopes were performed among groups, as well as before vs after AVF closure in group 1. For the latter, time was balanced before vs after AVF closure, with at least 10 observations per patient. Results The cohort included 285 KTR (Table 1), with a median follow-up of 1750 days [1665; 2028]. Focusing on group 1, AVF closure occurred after a mean time of 653 ± 441 days post Tx, with a thrombosis/ligation ratio of 19/95. Balanced study periods before vs after AVF closure lasted 15.7 and 14.9 months, respectively. No difference was found between corresponding intercepts (p, 0.11). By contrast, eGFR slopes were significantly different before (0.043 ml/min/year) versus after (-0.176 ml/min/year) AVF closure (p, 0.0115). Similar observations were obtained using FAS equation Conclusion A significant acceleration of eGFR decline is observed over the 15 months following the closure of functioning AVF in KTR. [less ▲]

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See detailBlood meal and blood products detection using Synchronous fluorescence spectroscopy
Lecrenier, Marie-Caroline ULiege; Abbas, Ouissam; Taira, Aurélien et al

Poster (2016, November)

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both ... [more ▼]

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both authorised in aqua feed, whereas only porcine blood products are allowed to be used in feed intended for other non-ruminants. Besides official methods (light microscopy and PCR), complementary methods are developed in order to refine the by-products identification. By-products derived from blood are one of these products for which additional information are needed. Indeed, these prohibited materials sometimes cannot be distinguished from those authorised (e.g. milk powder) using official methods. The aim of this work was to develop a fast and easy method to detect blood meal and blood products. This study was based on the detection of hemoglobin in animal feed by synchronous fluorescence spectroscopy (SFS). To achieve this goal, preliminary tests were carried out on reference material (hemoglobin and albumin purchased from Sigma Aldrich) in order to determine appropriate conditions (solvent) and parameters (offset values) for hemoglobin detection. Selected settings were then applied to analyse protein extracts of commercial feed material derived from blood. The results obtained on blood meal and blood products (hemoglobin powder and plasma powder) showed fluorescence spectral bands that characterise hemoglobin. In order to determine whether the method was fit for the purpose, real commercial compound feeds known to contain or to be free from blood products or blood meal were analysed. The Principal Component Analysis (PCA) applied to these spectra showed that it was possible to discriminate samples containing hemoglobin from those that do not contain. This result confirmed that SFS is a promising screening method for the detection of hemoglobin in animal feed. [less ▲]

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See detailMolecular and functional characterization of the unconventional interaction partners of CXCR7 and their role in virus-associated tumorigenesis
Meyrath, Max Marc Roger ULiege; Szpakowska, Martyna; Ollert, Markus et al

Poster (2016, November)

CXCR7 is an atypical and enigmatic chemokine receptor playing crucial roles in numerous physiological processes but also in viral infection and cancer. To date, the exact functions and signaling ... [more ▼]

CXCR7 is an atypical and enigmatic chemokine receptor playing crucial roles in numerous physiological processes but also in viral infection and cancer. To date, the exact functions and signaling properties of CXCR7 are still a matter of debate. Unlike other chemokine receptors, CXCR7 is proposed to act as a scavenging receptor for its ligands, to trigger arrestin-dependent signaling and possibly classical G-protein mediated signaling. Besides its two endogenous chemokines CXCL11 and CXCL12 and the virus-encoded chemokine vCCL2, accumulating evidence suggest that CXCR7 interacts with several non-chemokine ligands such as the pro-angiogenic peptide Adrenomedullin (ADM). Furthermore, calcitonin receptor like receptor (CLR) and receptor activity modifying proteins (RAMPs), constituting the main ADM receptor, might crosstalk with CXCR7 conferring the receptor alternative functions. While the interactions of CXCR7 with classical chemokine ligands are now well characterized, little is known about the interdependence of ADM and the membrane interacting partners with CXCR7, the signaling pathways activated as well as their roles in viral infection and tumorigenesis. The aims of this PhD project are to 1) unravel the role and impact of CLR and RAMPs on the binding, scavenging and signaling properties of CXCR7 towards the different ligands ADM, CXCL12, CXCL11 and vCCL2. Additionally, by using state-of-the-art techniques like Bioluminescence Resonance Energy Transfer (BRET), we will 2) inquire the ability of CXCR7 to heterodimerize with CLR and RAMPs. Lastly, since CXCR7 and RAMPs were described to be upregulated upon infection by several tumorigenic viruses, 3) a detailed expression profile of all the ligands and partners of CXCR7 in a herpesvirus-induced cancer model will be determined and will help to reveal the role of CXCR7 in viral associated tumorigenesis. This project will allow improving our level of understanding of CXCR7 regulation and functions and will open new therapeutic directions for selective CXCR7 inhibition. [less ▲]

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See detailP2X1 ion channel is critical for vascular integrity in inflammation
Wéra, Odile ULiege; Delierneux, Céline; Servais, Laurence ULiege et al

Poster (2016, November)

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See detailWet-aging vs. Dry-aging : influence sur la tendreté et la stabilité oxydative des viandes Charolaises
Didimo Imazaki, Pedro Henrique ULiege; Teixeira Gonçalves, André ULiege; Krantz, Mike et al

Poster (2016, November)

The aim of this study was to compare the effect of aging conditions (dry-aging vs. wet-aging) and time (7, 28, 49 and 70 days) on tenderness, color and lipid stability of Charolais beef. Three longissimus ... [more ▼]

The aim of this study was to compare the effect of aging conditions (dry-aging vs. wet-aging) and time (7, 28, 49 and 70 days) on tenderness, color and lipid stability of Charolais beef. Three longissimus dorsi of Charolais cows were dry- or wet-aged for up to 70 days at 2 °C. At different times, part of these samples was cut into steaks, vacuum packaged and stored during 4 days at 4 °C + 8 days at 8 °C. The following parameters were evaluated at different intervals: pH, tenderness (Warner–Bratzler peak shear force), color (CIE L*a*b*), myoglobin oxidation (K/S572:K/S525 ratio) and lipid oxidation (TBARS). No difference in tenderness was observed between the two aging techniques. The sensitivity of samples to oxidation was influenced by the aging technique (wet-aging > dry-aging). For the studied samples, aging beyond 28 days could be considered excessive from an organoleptical point of view. [less ▲]

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See detailLife cycle assessment of on-site accelerated food waste composter
Groslambert, Sylvie ULiege; Léonard, Angélique ULiege; Finet, Sébastien

Poster (2016, November)

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See detailImpact of postural education on child’s sitting position in classroom and on back pain
Fettweis, Tatiana ULiege; Nattier, Elodie; Vanderthommen, Marc ULiege

Poster (2016, November)

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See detailSpecific detection of blood derived products in animal feed using UPLC-MS/MS
Lecrenier, Marie-Caroline ULiege; Dieu, Marc; Veys, Pascal et al

Poster (2016, November)

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly ... [more ▼]

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly regulated. Blood meal and blood products (hemoglobin powder and plasma powder) of bovine origin are totally prohibited. Blood meal and blood products of porcine origin are authorised in aquafeed, whereas only blood products are allowed to be used in feed intended for other non-ruminant. The detection of the type of protein and the species of origin is therefore crucial to ensure feed safety. With the current official methods for the detection of PAPs, light microscopy and PCR, it is not always possible to specifically identify this type of protein source. The objective of our work was to set-up a routine mass spectrometry method for a qualitative detection of blood meal and hemoglobin powder of bovine and porcine origin in feed. The method was based on the detection of species-specific peptides biomarkers identified in a previous study by a non-targeted approach. All biomarkers were peptides of α and β hemoglobin subunits. Proteins were extracted using a TCA/acetone protein precipitation protocol followed by purification with a 2-D Clean-Up Kit (GE Healthcare, USA). After an in-solution trypsin digestion step, analyses were performed by liquid chromatography (Acquity system, Waters, UK) coupled with a triple quadrupole mass spectrometer (Xevo TQS, Waters, UK) with electrospray ionisation. The acquisition and processing of data was carried out by MassLynx software (v. 4.1, Waters) and multiple reaction monitoring (MRM) design was made using the open-source software Skyline (https://skyline.gs.washington.edu/labkey/project/home/software/Skyline/begin.view). Reference hemoglobin powder was used to select MRM transitions for each peptide biomarkers and to optimise their collision energy. Commercial feed material and compound feed containing or free from blood derived products were then analysed. Selected transitions were present in materials or feed known to contained blood derived product of the same origin and absent in the others. Artificially contaminated feed with various contamination levels were also analysed in order to evaluate the influence of matrix composition and to experimentally determine the limit of detection. A first estimation of the LOD was around 0.05 % w/w which was below the LOD imposed by the EC for animal proteins detection method. [less ▲]

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See detailThe BCCM/ULC collection to conserve the biodiversity and explore the applied potential of Polar cyanobacteria
Becker, Pierre; SZTERNFELD, P; ANDJELKOVIC, M et al

Poster (2016, October 28)

In the Polar Regions, Cyanobacteria represent key primary producers and are the main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial ... [more ▼]

In the Polar Regions, Cyanobacteria represent key primary producers and are the main drivers of the food webs in a wide range of aquatic to terrestrial habitats. For example, they build benthic microbial mats in lakes and soil crusts in terrestrial biotopes. They may present interesting features to survive freeze/thaw cycles, seasonally contrasted light intensities, high UV radiations, dessication and other stresses. The BCCM/ULC public collection funded by the Belgian Science Policy Office since 2011 aims to gather a representative portion of the polar cyanobacterial diversity with different ecological origins (limnetic mats, soil crusts, cryoconites, endoliths…). It makes it available for researchers to study the taxonomy, evolution, adaptations to harsh environmental conditions, and genomic make-up. It presently includes 226 cyanobacterial strains, with 119 being of Antarctic origin (catalogue: http://bccm.belspo.be/catalogues/ulc-catalogue-search). An ISO 9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. The morphological identification shows that the strains belong to the orders Synechococcales, Oscillatoriales, Pleurocapsales, Chroococcidiopsidales and Nostocales. The 16S rRNA and ITS sequences of the strains are being characterized. Our results show that the Antarctic strains are positioned into 25 OTUs (sequences with > 97,5% 16S rRNA similarity), and thus, represent a quite large diversity. In addition, cyanobacteria are known to produce a wide range of secondary metabolites (e.g. alkaloids, cyclic and linear peptides, polyketides) with bioactive potential. Among these bioactive metabolites, some display antibiotic, anticancer or antifungal effects. In collaboration with the BCCM/IHEM collection of biomedical fungi, a screening of cyanobacterial strains from BCCM/ULC was performed in order to discover potential new antifungal drugs. The analysis of a first set of methanol extracts from 15 different strains put in evidence the antifungal activity of a Phormidium priestleyi isolate. The latter remains active up to 0.5% (v/v) of fungal culture and was able to inhibit the growth of various fungal species among Candida, Cryptococcus, Aspergillus, and Penicillium. The raw extract was subjected to HPLC and a fraction containing the active molecule was obtained. This molecule appeared to be a thermostable hydrophobic compound. Moreover, in vitro toxicological analyses suggest that the compound has a general cytotoxic effect that could be inhibited by the mammalian metabolism. Further analyses are needed to identify the molecule and to determine if it could be a candidate for a new antifungal drug. In summary, the BCCM/ULC public collection serves as a Biological Resource Centre to conserve ex situ and document the biodiversity of polar cyanobacteria, as well as a repository for discovery of novel bioactive compounds. [less ▲]

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See detailThe ‘cyanobiome’ of Svalbard, High Arctic
Stelmach Pessi, Igor ULiege; Laughinghouse, H Dail; Velazquez, David et al

Poster (2016, October 28)

Over the last decades, the Arctic has experienced a warming trend that is nearly twice as high as the global average, a phenomenon known as ‘Arctic amplification’. The impact of warmer temperatures on ... [more ▼]

Over the last decades, the Arctic has experienced a warming trend that is nearly twice as high as the global average, a phenomenon known as ‘Arctic amplification’. The impact of warmer temperatures on Arctic ecosystems is still unclear. Cyanobacteria are the key primary producers in freshwater and terrestrial Arctic ecosystems, where they are the driver for numerous ecological functions. For a better understanding of the impacts of climate change on Arctic ecosystems, baseline knowledge on cyanobacterial diversity and distribution is crucial. Here we investigate, for the first time, the biogeographic patterns of cyanobacterial communities across Svalbard, using 454 pyrosequencing of partial 16S rRNA gene sequences. Samples were taken from distinct ecosystems and biogeographic zones. We also compare the studied communities with similar Antarctic communities. [less ▲]

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See detailSilurian palynomorphs from the Precordillera basin, western Argentina: biostratigraphy and diversity trends
Garcia Muro, V.J.; Rubinstein, C.V.; Steemans, Philippe ULiege

Poster (2016, October 27)

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See detailA benchmark for urban flood modelling
Arrault, Anaïs; Finaud-Guyot, Pascal; Archambeau, Pierre ULiege et al

Poster (2016, October 25)

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See detailSpecific interests in Autism with versus without speech onset delay : the importance of perceptually versus thematically organized interests
Chiodo, Liliane ULiege; Eusèbe, Sandrine; Majerus, Steve ULiege et al

Poster (2016, October 25)

To explore whether autistic adults with versus without speech delay also differ in the perceptual vs thematic nature of their specific interests.

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See detailThermochemical conversion of sugar industry by-products to biofuel
Nicodème, Thibault ULiege; Jacquet, Nicolas ULiege; Richel, Aurore ULiege

Poster (2016, October 24)

Poster concernant la conversion des co-produits de l'industrie sucrière en biocarburants via la voie thermochimique. Description des procédés tels que la gazéification, Fischer-Trospch, la pyrolyse.

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See detailComportement du puceron du pois face aux volatiles du basilic.
Yarou, Boni Barthélémy ULiege; Boullis, Antoine ULiege; Verheggen, François ULiege et al

Poster (2016, October 22)

Les pucerons font partie des insectes qui causent des dégâts d’importances économiques sur une grande diversité de plantes cultivées. Les insecticides de synthèse généralement utilisés pour les contrôler ... [more ▼]

Les pucerons font partie des insectes qui causent des dégâts d’importances économiques sur une grande diversité de plantes cultivées. Les insecticides de synthèse généralement utilisés pour les contrôler montrent de plus en plus leurs limites (résistance), et sont néfastes pour l’homme et l’environnement. Dans la recherche de méthodes de lutte alternatives, le comportement de choix d’Acyrthosiphon pisum Harris (Homoptera : Aphididae) exposé à des sources d’odeurs de plant de fèves (Vicia fabae L.) et du basilic européen (Ocimum basilicum L.) à l’aide d’un olfactomètre en Y a été étudié. Les observations de cette expérience montrent que les pucerons se dirigent préférentiellement vers la source d’odeurs de fève et y passent plus de temps. Ce résultat montre que les volatiles du basilic ont un effet répulsif sur les pucerons du pois. D’autres tests seront évalués sur des espèces de pucerons plutôt généralistes afin de valider l’effet répulsif du basilic sur les pucerons. [less ▲]

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See detailImpacts de champignons entomopathogènes sur la biologie et le comportement de la punaise diabolique (Hemiptera, Pentatomidae)
Fingu Mabola, Junior Corneille ULiege; Serteyn, Laurent ULiege; Bawin, Thomas ULiege et al

Poster (2016, October 22)

Halyomorpha halys Stål, la punaise diabolique, est une espèce invasive préoccupante en provenance d’Asie de l’Est. En moins de 15 années, elle a colonisé près de l’ensemble des Etats-Unis d’Amérique ... [more ▼]

Halyomorpha halys Stål, la punaise diabolique, est une espèce invasive préoccupante en provenance d’Asie de l’Est. En moins de 15 années, elle a colonisé près de l’ensemble des Etats-Unis d’Amérique, occasionnant des pertes de rendement considérables dans les vergers et les cultures en champs ainsi que des nuisances dans les habitations durant l’hiver. La punaise diabolique a été recensée en Suisse en 2007, et il est à craindre qu’elle colonise la presque totalité de l’Europe d’ici 2020. Cette étude a pour objectif de prévenir l’installation de la punaise diabolique dans les pays européens en se focalisant sur le contrôle biologique. Des scientifiques américains ont développé une stratégie « attirer et tuer » basée sur la phéromone d’agrégation de la punaise diabolique et des pesticides. D’autres ont montré l’efficacité d’un champignon pathogène d’insectes (Beauveria bassiana strain GHA. Afin d’établir un contrôle rapide du ravageur en Europe, compatible avec l’agriculture biologique, une idée serait de combiner l’effet attractif de la phéromone d’agrégation et l’application de spores de B. bassiana. Dans cette étude, l’impact du champignon sur la mortalité de l’insecte et son autodissémination sont évalués. [less ▲]

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See detailSurgical treatment of mucometra in an Alpine Goat.
Casalta, Hélène ULiege; Knapp, Emilie ULiege; Evrard, Laurence ULiege et al

Poster (2016, October 21)

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Peer Reviewed
See detailReported post-operative analgesic administration to non-human primates: 2010 - 2015
Bertrand, Henri ULiege; Flecknell, Paul; Brady, James et al

Poster (2016, October 21)

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Peer Reviewed
See detailNon-compliance with STAIRS guidelines in stroke models
Thomas, Aurélie ULiege; Detilleux, Johann ULiege; Sandersen, Charlotte ULiege

Poster (2016, October 21)

Detailed reference viewed: 17 (4 ULiège)
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Peer Reviewed
See detailUtilization of Rice Distiller’s By-product for Swine Production in Northern Vietnam
Nguyen Cong, Oanh ULiege; Dang, Pham Kim; Luc, Do Duc et al

Poster (2016, October 21)

Detailed reference viewed: 18 (1 ULiège)
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Peer Reviewed
See detailPhysiological Responses to training and competition in 1 to 4 star eventing horses
Kirsch, Katharina ULiege; Düe, M.; Holzhausen, H. et al

Poster (2016, October 21)

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See detailMetabolites from media supplemented with 3’-sialyllactose and fermented by bifidobacteria have an antivirulent effect against intestinal pathogens
Bondue, Pauline ULiege; Daube, Georges ULiege; Delcenserie, Véronique ULiege

Poster (2016, October 21)

Complex oligosaccharides from human milk (HMO) promote growth of Bifidobacterium bifidum. Oligosaccharides from cow milk (BMO), similar to HMO, are mainly represented in colostrum by 3’-sialyllactose ... [more ▼]

Complex oligosaccharides from human milk (HMO) promote growth of Bifidobacterium bifidum. Oligosaccharides from cow milk (BMO), similar to HMO, are mainly represented in colostrum by 3’-sialyllactose (3’SL). Bifidobacterium crudilactis, a species from bovine origin and encoding for β galactosidases and α-glucosidases, could be able to metabolise them. Also, fermentation products could have antivirulent activity against intestinal pathogens. This study focused on capacity of bifidobacteria to metabolise 3’SL and on potential antivirulent effect of cell-free spent media (CFSM) against pathogenic bacteria. B. bifidum BBA1 and B. crudilactis FR/62/B/3 isolated respectively from breastfed children feces and cow raw milk cheese were grown on media supplemented with 3’SL as sole source of carbon. Next, CFSM effects were tested against virulence gene expression using ler and hilA promoter activity of luminescent constructs of Escherichia coli 0157:H7 ATCC 43888 and Salmonella Typhimurium SA 941256, respectively. The effect was confirmed on wild type strains of E. coli O157:H7 ATCC 43890 and S. Typhimurium ATCC 14028 using RT-qPCR. Both strains were able to grow in presence of 3’SL. CFSM resulted in under-expression of hilA and ler genes for the luminescent constructs and in under-expression of ler (ratios of -15.4 and -8.1) and qseA (ratios of -2.1 and -3.1) genes for the wild type strain of E. coli O157:H7. No effect was observed with S. Typhimurium. Little is known about CFSM metabolites and they have to be isolated and identified. The potential synbiotic effect between 3’SL and bifidobacteria will be tested using the Shime®, a human gastrointestinal model. [less ▲]

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See detailDevelopment of an analytical method for the simultaneous measurement of 10 biogenic amines in food
Douny, Caroline ULiege; Benmedjadi, Soumaya; Blaszczyk, Marie et al

Poster (2016, October 21)

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See detailInbreeding and genomic runs of homozygosity in Vietnamese local chickens
Moyse, Evelyne ULiege; Nguyen Van, Duy; Vu Dinh, Ton et al

Poster (2016, October 21)

Most researches on genetic variety evaluation in Vietnamese local chicken breeds have been based up to now on microsatellite markers (Cuc et al., 2011; Pham et al., 2013). Thanks to the genotyping of ~580 ... [more ▼]

Most researches on genetic variety evaluation in Vietnamese local chicken breeds have been based up to now on microsatellite markers (Cuc et al., 2011; Pham et al., 2013). Thanks to the genotyping of ~580,000 SNPs on 95 chickens, this study aimed to assess the genetic diversity in four Vietnamese local breeds (Mia, Mong, Ho and Dong Tao). Inbreeding was examined through the detection of runs of homozygosity (ROH; Curik et al., 2014). The ROHs are long stretches of homozygous genome that are likely to reflect autozygosity. Given that Ho chickens have a small effective population size, more inbreeding and consequently longer ROHs are expected. Conversely, Mia chickens, with a larger effective population size should show less consanguinity and shorter ROHs. As expected, Mia chickens led to less observed homozygosity and less ROHs than the three other breeds. Moreover, when ROHs were subdivided in different lengths, the proportion of individuals having at least one R OH longer than 5Mb was significantly higher in Ho (63%) than in Mia chickens (33%). Given that very long ROHs are particularly associated with recent inbreeding, it seems that many Ho chickens have consanguineous parents. However, no significant difference was observed between the four breed concerning the total length of long ROHs (> 5Mb). In conclusion, the analysis of ROHs reported less consanguinity in Mia than in Ho chickens. In each breed, some individuals are found more consanguineous than expected. This study also confirms that ROHs are a useful tool in inbreeding research. [less ▲]

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See detailEffect of aging time, aging technique (dry- vs. wet-aging) and packaging on tenderness, pigment and lipid stability of Belgian blue beef
Didimo Imazaki, Pedro Henrique ULiege; Teixeira Gonçalves, André ULiege; Krantz, Mike et al

Poster (2016, October 21)

The aim of this study was to compare the effect of aging technique (dry-aging and wet-aging), aging time (0, 21, 42 and 63 days) and packaging during display (vacuum and shrinkable film wrapping) on pH ... [more ▼]

The aim of this study was to compare the effect of aging technique (dry-aging and wet-aging), aging time (0, 21, 42 and 63 days) and packaging during display (vacuum and shrinkable film wrapping) on pH, tenderness, pigment and lipid stability of beef. Three longissimus dorsi muscles from two Belgian Blue cows were dry- or wet-aged for up to 63 days at 2 °C. At different times, part of these samples was cut into steaks and repackaged in vacuum bags or shrinkable film, and stored during 4 days at 4 °C + 8 days at 8 °C (simulated retail display). The following parameters were evaluated at different intervals: pH, tenderness (Warner–Bratzler shear force), color (CIE L*a*b*), myoglobin oxidation (K/S 572/525 ratio) and lipid oxidation (TBARS). The aging technique and the packaging during simulated retail display had an effect on pH (dry-aging > wet-aging, P < 0.05; shrinkable film > vacuum, P < 0.05). An increase of tenderness was observed during the first 21 days of aging (P < 0.05). The sensitivity of samples to pigment oxidation was influenced by the packaging during display (shrinkable film > vacuum, P < 0.05). Aging time and packaging during display increased lipid oxidation (42 and 63 > 0 and 21 days, P < 0.05; plastic wrap > vacuum, P < 0.05). This study contributes new knowledge about Belgian Blue beef behavior whether it is wet- or dry-aged. Further research will be conducted to study the antioxidant capacity of these meats in order to better understand the oxidation process. [less ▲]

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See detailInbreeding and genomic runs of homozygosity in Vietnamese local chickens
Nguyen Van Duy, ULiege; E. Moyse; Vu D. T. et al

Poster (2016, October 21)

Most researches on genetic variety evaluation in Vietnamese local chicken breeds have been based up to now on microsatellite markers (Cuc et al., 2011; Pham et al., 2013). Thanks to the genotyping of ~580 ... [more ▼]

Most researches on genetic variety evaluation in Vietnamese local chicken breeds have been based up to now on microsatellite markers (Cuc et al., 2011; Pham et al., 2013). Thanks to the genotyping of ~580,000 SNPs on 95 chickens, this study aimed to assess the genetic diversity in four Vietnamese local breeds (Mia, Mong, Ho and Dong Tao). Inbreeding was examined through the detection of runs of ho- mozygosity (ROH; Curik et al., 2014). The ROHs are long stretches of homozygous genome that are likely to re ect autozygosity. Given that Ho chickens have a small e ective population size, more inbreeding and consequently longer ROHs are expected. Conversely, Mia chickens, with a larger e ective population size should show less consanguinity and shorter ROHs. As expected, Mia chickens led to less observed homozygosity and less ROHs than the three other breeds. Moreover, when ROHs were subdivided in dif- ferent lengths, the proportion of individuals having at least one R OH longer than 5Mb was signi cantly higher in Ho (63%) than in Mia chickens (33%). Given that very long ROHs are particularly associated with recent inbreeding, it seems that many Ho chickens have consanguineous parents. However, no signi cant di erence was observed between the fourbreed concerning the total length of long ROHs (> 5Mb). In conclusion, the analysis of ROHs reported less consanguinity in Mia than in Ho chickens. In each breed, some individuals are found more consanguineous than expected. This study also con rms that ROHs are a useful tool in inbreeding research. [less ▲]

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See detailImpact of skimming and microfiltration processes on equol concentration in milk
Daems, Frédéric ULiege; Ninane, Véronique; Romnee, Jean-Michel et al

Poster (2016, October 19)

Equol is a microbial metabolite of isoflavones that could be used as therapeutic agent against several diseases and cancers. Cow's milk could be a potential source of equol in the human diet, but more ... [more ▼]

Equol is a microbial metabolite of isoflavones that could be used as therapeutic agent against several diseases and cancers. Cow's milk could be a potential source of equol in the human diet, but more studies are needed on the changes in equol concentration during the technological processing of milk and dairy products. This first exploratory study sought to assess the impact of industrial skimming and microfiltration processes on equol concentration in conventional cow's milk. The milk samples used in this study came from bulk cow’s milk collected in Wallonia (Belgium), by a local dairy and processed in a local cheese factory. Six random sampling were conducted during spring and, for each of them, samples from raw, skimmed and microfiltered milk were analyzed. Equol was present in all samples at a concentration of between 3.2 and 10.3 µg.L-1. A Wilcoxon's signed rank test was then performed on the difference of data, having raw milk as reference, irrespective of the collection date. The results showed that the skimming process slightly increased the equol concentration in milk and therefore that equol had no or little affinity with the lipid milk fraction. The results also showed that, with the microfiltration process, a small proportion of equol was retained. Equol concentration has returned to the same level as that found in raw milk. This might be because of a chemical affinity either with bigger molecules that are physically retained or through direct interaction with the membrane. This scoping study paves the way for more extensive studies on the interaction between equol and other components of the milk. [less ▲]

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See detailDevelopment and evaluation of real-time PCR targets for the detection of insect in feed
Gerard, Amaury ULiege; Debode, Frédéric; Marien, Aline et al

Poster (2016, October 19)

Insects are rich in proteins and could be an alternative source of proteins to feed animals. Numerous companies started the production of insects at small or larger scale as feed for chicken and fish ... [more ▼]

Insects are rich in proteins and could be an alternative source of proteins to feed animals. Numerous companies started the production of insects at small or larger scale as feed for chicken and fish. Most of the business models for feed production are based on the black soldier fly (Hermetia illucens) or the mealworm (Tenebrio molitor). In Europe, these novel feed are not yet authorized and products are commercialized outside Europe or eventually used as pet food (e.g. wild birds). For further authorization in Europe, many questions must be clarified concerning the presence of antinutritional compounds, the risk associated to pathogens, to residues (pesticides, antibiotics, heavy metals) and to allergens. To authorize such products on the market, methods to detect if a product really contains insects and to authenticate insect products are also mandatory. European Commission Regulation No 51/2013 named the Polymerase Chain Reaction as a reference method to determine the constituents of animal origin in feed. Targets focused on insects (target common to all insects) and targets specific to particular insect species are required. PCR methods are developed at CRA-W in this way. Among the methods developed, three of them already gave interesting results. The first one (81 bp) is specific to all insects excepting individuals from the Diptera order. The two others (94 bp and 114 bp) are specific to Tenebrio molitor. The specificity of the targets was tested against 45 insect species and on 10 commercial insect-based feed products (real-processed feed material samples). The sensitivity of the method was assessed through the AFNOR XP V03-020-2 standard approach using the LOD6 method. The three methods reached the recommended performance criteria (LOD≤ 20 copies). [less ▲]

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See detailMobility of 137Cs, 10Be and 210Pb in soil as a function of the soil texture, organic matter content and pH
de Tombeur, Félix ULiege; Cornu, Sophie; Bourlès, Didier et al

Poster (2016, October 18)

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ... [more ▼]

Vertical matter transfer processes (bioturbation and <2μm translocation) in soils and their dynamics are up to now poorly constrained although they are responsible for the temporal evolution of the ecosystem services. In order to better constrain and quantify these processes, a kinetic quantification of the involved transfers based on vertical profiles of 210Pb (xs), 10Be and 137Cs has been developed for Luvisols in which soluble transfers could be neglected. The range of applicability of this type of approach was nevertheless not tested. In this work, we analyzed 210Pb (xs), 10Be and 137Cs in soils exhibiting gradients of organic matter, pH and < 2 μm fraction (Podzol, Andosol Ferralsol, Leptosol). Acidic soils and large organic matter contents were considered. The depth distribution of the studied isotopes was then interpreted as a function of the organic matter content, the soil pH and its < 2 μm fraction. This demonstrates that 10Be and 137Cs losses occur under soluble form in the Podzol, especially under forest, more acid, for the latter. Soluble 10Be transfers were also identified in the Andosol when they were considered as negligible in the Leptosol. For 210Pb (xs), soluble transfers are also probable for soils with pH less than 5.5 (Andosol and Ferralsol) and strong affinity for organic matter has been demonstrated. Thus, under acidic conditions and low < 2 μm fraction, soluble transfer cannot neglected anymore. [less ▲]

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See detailTowards precision beta-decay measurements with laser-cooled argon-35
Lenaers, Florence ULiege; Glover, Rohan ULiege; Bastin, Thierry ULiege

Poster (2016, October 18)

Laser cooling and trapping techniques enjoy a solid reputation as powerful and elegant providers of well-localized, backing-free sources. Not only does this hold true within the atomic physics and quantum ... [more ▼]

Laser cooling and trapping techniques enjoy a solid reputation as powerful and elegant providers of well-localized, backing-free sources. Not only does this hold true within the atomic physics and quantum optics communities, but also beyond. Most notably, these techniques have recently joined the toolbox of low-energy, high-precision tests of the Standard Model. Our work with argon falls within the subset of nuclear beta decay studies, which have historically played a key role in establishing and testing our understanding of weak-interaction physics. In this framework, precision measurements of the beta asymmetry correlation parameter in the beta decay of Ar-35 have been identified as one promising probe for physics beyond the Standard Model. Our current work focuses on the development of a test magneto-optical trap (MOT) setup for Ar-40 as a preliminary step towards cooling and trapping Ar-35 atoms. In particular, much effort is being put into optimizing the efficiency of our source apparatus, which is crucial to achieving high enough statistics in the final trap. We present the current status of the experimental realization of our setup. Measuring the beta asymmetry correlation parameter requires highly spin-polarized samples. Therefore, in parallel to our experimental work, we are numerically investigating methods for generating, controlling and precisely measuring the degree of spin polarization of a sample of Ar-35 atoms. We also aim at being able to quickly switch between a polarized and a non-polarized sample so as to better understand systematic effects. We report on the progress of our theoretical modeling. [less ▲]

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Peer Reviewed
See detailA one year survey of seagrass primary productivity using the diving-PAM technique
Richir, Jonathan ULiege; Abadie, Arnaud; Grosjean, Philippe et al

Poster (2016, October 18)

Marine magnoliophytes are major primary producers in coastal benthic habitats worldwide. They play a crucial role in the global carbon cycle (one of the more efficient blue carbon wells). Hence, it is ... [more ▼]

Marine magnoliophytes are major primary producers in coastal benthic habitats worldwide. They play a crucial role in the global carbon cycle (one of the more efficient blue carbon wells). Hence, it is necessary to characterise the eco-systemic services seagrass meadows provide. Posidonia oceanica (L.) Delile, the main Mediterranean seagrass species, has high foliar and belowground biomass production. Several methods have been used so far to measure its primary production (e.g., using incubation bells, optodes, biomass and elementary content measurements). A less used method relies on chlorophyll fluorescence measurements through the Pulse Amplitude Modulated (PAM) fluorometry method (Diving - PAM). In the framework of the STARECAPMED project, this study aimed to determine weekly to bimonthly over a one-year period the photosynthetic responses (Yield, relative Electron Transfer Rate, Rapid Light Curve) of P. oceanica. The survey was performed at 10m depth in a pristine meadow (Calvi, Corsica, France). To obtain reliable and comparable data, the protocol was standardized: measurements were performed on the convex middle part of the third leaf, at zenith, during shiny and calm weather days. Results showed that the plant displayed a well-marked seasonality. The mean ETR (μmol e- m-2 s-1 ) of the plant ranged from 2.17 in winter to 21.9 in summer and was linearly correlated throughout the year with the in situ irradiance (PAR irradiance taken perpendicularly to the surface, in the average leaf orientation). The ETR plateaus of the RLCs, ranging from 10.9 to 35.0, and their corresponding maximum PAR intensities evolved similarly. These results demonstrated both the adaptation and the seasonal plasticity of the meadow’s photosynthetic system. Overall the non-destructive PAM technique is a powerful and cost-effective tool to assess the primary productivity of seagrass meadows where other techniques (e.g. optodes) cannot be used and when direct sampling (e.g. biomass measurements) is not allowed. [less ▲]

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See detailDiversity, dynamics and trophic ecology of animal communities associated to Posidonia oceanica (L.) Delile macrophytodetrital accumulation: synthesis of a ten year study
Lepoint, Gilles ULiege; Borges, Alberto ULiege; Champenois, Willy ULiege et al

Poster (2016, October 17)

In the Mediterranean, Neptune grass Posidonia oceanica, produces a huge quantity of detrital biomass. These macrophytodetritus may accumulate in shallow waters, forming litter accumulations colonised by ... [more ▼]

In the Mediterranean, Neptune grass Posidonia oceanica, produces a huge quantity of detrital biomass. These macrophytodetritus may accumulate in shallow waters, forming litter accumulations colonised by abundant, yet understudied, animal communities. These accumulations are especially foraged by juvenile and adult fishes. Here, we aim to synthesize results obtained over the last ten years regarding diversity, dynamics and trophic ecology of associated meio- and macrofauna. Accumulations are found throughout the year but important seasonal and short-term variability in composition, quantity and physico-chemical parameters inside the accumulation is observed. Accumulations are dominated by respiration (litter degradation), however, primary production occurs at exposed surfaces (epiphytic production). Meio- and macrofauna have distinct traits in comparison to adjacent habitats (seagrass meadows or epilithic algae communities). A physico-chemical gradient occurs inside accumulations which partially defines assemblage composition and distribution. Meiofauna, in particular harpacticoid copepods, is diverse, abundant and composed of species from seagrass meadows, water column and sediment. In contrast, macrofaunal assemblages are simplified compared to the ones occurring in the seagrass meadows and are dominated by amphipods. Litter accumulations display a lower macrofaunal diversity than do seagrass meadows, but a higher abundance and animal biomass. Meio- and macrofauna show a high trophic diversity, dominated by ingestion and assimilation of epiphytes (macroalgae and, probably, detrivorous microbiota). Moreover, direct or indirect assimilation of carbon originating from seagrass detritus is demonstrated for many species. Although diverse trophic niches were observed, the assemblage showed a simplified trophic web structure compared to the seagrass meadows. Detritivorous organisms dominate this assemblage and are more abundant in the litter than in the living meadows. Consequently, according to its abundance and the fact it consumes directly and indirectly seagrass material, fauna associated to litter accumulation may play a significant role in the degradation and transfer to higher trophic level of detrital seagrass carbon. [less ▲]

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See detailNovel innovative possibilities for the dairy industry opened by common format of FT-MIR instruments
Grelet, Clément ULiege; Fernandez Pierna, Juan Antonio; Dardenne, Pierre et al

Poster (2016, October 17)

FT-MIR technology is worldwide used for fast and cost effective determination of major milk components. However, due to the different individual response of each instrument the potential of this ... [more ▼]

FT-MIR technology is worldwide used for fast and cost effective determination of major milk components. However, due to the different individual response of each instrument the potential of this technology is currently underexploited as new tools cannot be easily ported to other instruments. Recently a standardization method was developed in order to harmonize the spectral response format between instruments of different brands and models but also across time for each spectrometer. The method matches monthly the infrared response of all spectrometers on the response of a reference instrument, making all machines talking a common language. The objective is to allow the creation and the use of common, new and innovative concepts by pooling resources and sharing data. Using this method, new tools for analysis of milk quality and milk technological properties have been created and shared within the network, as fatty acids and minerals predictions or milk coagulation properties. New concepts requiring a common spectral format have been developed like the untargeted detection of milk contaminant and abnormal milk or the determination of milk geographic origin. Models in relation with the status of the dairy cow were also created and shared as to predict ketosis, negative energy balance or methane emissions. Therefore models can be developed at one place and deployed within the entire network, in which 90 instruments are currently monthly standardized. [less ▲]

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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Munaut, Carine ULiege et al

Poster (2016, October 14)

Detailed reference viewed: 20 (4 ULiège)
See detailDifferent and synergistic effect of 5-Azacytidine and Rapamycin on ex vivo expansion of natural human T Regulatory cells
Conteduca, Giuseppina ULiege

Poster (2016, October 14)

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are ... [more ▼]

Natural T regulatory cells (Treg) are challenging to expand ex vivo, and this has severely hindered in vivo evaluation of their therapeutic potential. 5-Azacytidine (5-azaC) and Rapamycin (RAPA) are immunosuppressive drugs that promote selectively the expansion of CD4+CD25highFoxp3+ regulatory T cells.: We investigated whether 5-azaC and RAPA could be used together to promote the ex vivo expansion of Tregs purified from adult human peripheral blood.We found that 5-azaC helped maintain FOXP3 expression during the expansion process probably by promoting the conversion of T conventional (Tconv) in Treg, instead Rapa induces selectively apoptosis in Tconv cells and expansion in Treg. Addition of 5-azaC to RAPA treated cultures improved gene expression of FOXP3, CD25, STAT5 and TGF-B resulted in enhanced Treg expansion and suppressive activity. Also Rapa and 5-AzaC combination sustain Bcl-2 protein expression in Treg conferring resistance to apoptosis process. 5-azaC may have utility in ex vivo expansion of human Tregs, not as a single agent, but in combination with RAPA. These data may considerably accelerate the development of immunotherapeutic approaches for the treatment of autoimmune disease or posttransplant alloreactions by the adoptive transfer of nTreg cells [less ▲]

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See detailAzacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects
Ehx, Grégory ULiege; Fransolet, Gilles ULiege; de Leval, Laurence ULiege et al

Poster (2016, October 14)

The demethylating agent 5-azacytidine (AZA) has proven its efficacy as treatment for myelodysplastic syndrome and acute myeloid leukemia. In addition, AZA can demethylate FOXP3 intron 1 (FOXP3i1) leading ... [more ▼]

The demethylating agent 5-azacytidine (AZA) has proven its efficacy as treatment for myelodysplastic syndrome and acute myeloid leukemia. In addition, AZA can demethylate FOXP3 intron 1 (FOXP3i1) leading to the generation of regulatory T cells (Treg). Here, we investigated the impact of AZA on xenogeneic graft-versus-host disease (xGVHD) and graft-versus-leukemia effects in a humanized murine model of transplantation (human PBMCs-infused NSG mice), and described the impact of the drug on human T cells in vivo. We observed that AZA improved both survival and xGVHD scores. Further, AZA significantly decreased human T-cell proliferation as well as IFN-γ and TNF-α serum levels, and reduced the expression of GRANZYME B and PERFORIN 1 by cytotoxic T cells. In addition, AZA significantly increased Treg frequency through hypomethylation of FOXP3i1 as well as increased Treg proliferation. The later was subsequent to higher STAT5 signaling in Treg from AZA-treated mice, which resulted from higher IL-2 secretion by conventional T cells from AZA-treated mice itself secondary to demethylation of the IL-2 gene promoter by AZA. Importantly, Tregs harvested from AZA-treated mice were suppressive and stable over time since they persisted at high frequency in secondary transplant experiments. Finally, graft-versus-leukemia effects (assessed by growth inhibition of THP-1 cells, transfected to express the luciferase gene) were not abrogated by AZA. In summary, our data demonstrate that AZA prevents xGVHD without abrogating graft-versus-leukemia effects. These findings could serve of basis for further studies of GVHD prevention by AZA in acute myeloid leukemia patients offered an allogeneic transplantation. [less ▲]

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See detailDesign and synthesis of Fe2O3 nanoparticles/N-doped porous carbon structures as high performance electrode for lithium ion battery
Alkarmo, Walid ULiege; Ouhib, Farid ULiege; Aqil, Abdelhafid ULiege et al

Poster (2016, October 13)

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous ... [more ▼]

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous frameworks have emerged as attractive materials for various electrochemical energy storage/conversion devices, including Li-ion batteries (LIBs), Li−S batteries, supercapacitors and fuel cells. In this context, a hierarchically structured macro- and mesoporous N-doped carbon with dispersed Fe2O3 nanoparticles (NDC@Fe2O3) is prepared by thermal treatment of a novel composite composed by PMMA particles decorated by graphene oxide (GO), PPy and iron salts. The NDC@Fe2O3 composite exhibited high surface area with a hierarchical pores structure. The combination of Fe2O3 nanoparticles with porous carbon to form hybrid anode has been an efficient way to maintain the electronic integrity of the whole electrode since the carbon acts as a buffer layer to accommodate the volume variation and to provide multidimensional electron transport pathways during the charge/discharge process. [less ▲]

Detailed reference viewed: 143 (13 ULiège)
See detailReal-time capture of the folding-unfolding transitions in a single oligorotaxane foldamer
Sluysmans, Damien ULiege; Hubert, Sandrine ULiege; Bruns, Carson et al

Poster (2016, October 13)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULiege; Mendoza Gallego, Carlos ULiege; Lismont, Marjorie ULiege et al

Poster (2016, October 13)

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side ... [more ▼]

Development of photochemistry in macroscopic batch reaction vessels is hampered due to inherent limitations: light penetration remains superficial, resulting in inhomogeneous irradiation and hence to side-reactions or product degradation due to overexposure. The recent implementation of photochemical processes in microreactors under continuous-flow conditions appeared to be much more powerful than its batch analogue in terms of irradiation efficiency and light penetration. Furthermore, the fine control of residence time ensures an accurate control of the irradiation time, avoiding side-reactions and degradation. The photooxidation of (L)-methionine with singlet oxygen using Rose Bengal as a sensitizer was successfully implemented in a microreactor setup and led to total and selective conversion into methionine sulfoxide, an important building block for the organic synthesis of peptides or functionalized amino acid. The reaction was performed in 20 min while the same reaction in batch took 2h. [less ▲]

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See detailThe concept of the diffusion calibration for accurate drift time measurement by traveling wave ion mobilty
Kune, Christopher ULiege; Far, Johann ULiege; De Pauw, Edwin ULiege

Poster (2016, October 13)

Ion mobility spectrometry (IMS) is a gas phase separation technique which relies on differences in the collisional cross section (CCS) of ions. Ionic clouds of unresolved conformers overlapped if the CCS ... [more ▼]

Ion mobility spectrometry (IMS) is a gas phase separation technique which relies on differences in the collisional cross section (CCS) of ions. Ionic clouds of unresolved conformers overlapped if the CCS difference is below the instrumental resolution expressed as Ω/ΔΩ. The experimental arrival time distribution (ATD) peak is then a superimposition of the various contributions weighted by their relative intensities. We have developed a strategy for accurate drift time determination using traveling wave ion mobility spectrometry (TWIMS) of poorly and unresolved conformers. This method implements through a calibration procedure the link between the peak full width at half maximum (FWHM) and the drift time of model compounds for wide range of settings for wave heights and velocities. We modified a Gaussian equation which achieves the deconvolution of ATD peaks where the FWHM is fixed according to our calibration procedure. The new fitting Gaussian equation only depends on two parameters: The apex of the peak (A) and the mean drift time value (µ). The standard deviation parameter (correlated to FWHM) becomes a function of the drift time. This correlation function between µ and FWHM is obtained using the TWIMS calibration procedure which determines the maximum instrumental ion beam diffusion using ionic compounds which are detected as single conformers in the gas phase. This deconvolution process has been used to highlight the presence of poorly resolved conformers for couples of crown ethers and peptides leading to CCS determination in better agreement with quantum chemistry predictions. [less ▲]

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See detailAlkylcobalt as source of radicals for the controlled polymerization of less activated vinyl monomer
Demarteau, Jérémy ULiege; Cordella, Daniela ULiege; Kermagoret, Anthony et al

Poster (2016, October 13)

Detailed reference viewed: 21 (4 ULiège)
See detailSynthesis and characterization of non-isocyanate polyurethane (NIPU) hydrogels
Gennen, Sandro ULiege; Grignard, Bruno ULiege; Thomassin, Jean-Michel ULiege et al

Poster (2016, October 13)

Due to its good biocompatibility, biodegradation and excellent mechanical properties, polyurethane (PU) is a material of choice for biomedical applications (gloves, tubing, artificial membranes…) and, as ... [more ▼]

Due to its good biocompatibility, biodegradation and excellent mechanical properties, polyurethane (PU) is a material of choice for biomedical applications (gloves, tubing, artificial membranes…) and, as hydrogels, it was used as wound dressing, soft contact lenses, drug delivery systems and scaffolds for tissue engineering. Classically, PU are synthesized by a step-growth polymerization between poly (di-)ols and poly (di-) isocyanates. Due to the toxicity of isocyanates, REACH regulations have changed and, today, there is a need to develop greener and safer route to produce isocyanate-free PUs. This contribution focus on the synthesis of NIPU hydrogels by copolymerizing a bifunctional CO2-sourced hydrophilic PEG bi-cyclic carbonate with diamines in presence of a cross- linker. The PEG bi-cyclic carbonates was prepared via a CO2/epoxide coupling reactions using a new efficient organocatalytic system based on the use of an ammonium salt (TBAI) in combination with a fluorinated alcohol. [less ▲]

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See detailPILs as a new active material for radical organic batteries
Aqil, Mohamed ULiege; Ouhib, Farid ULiege; Aqil, Abdelhafid ULiege et al

Poster (2016, October 13)

Detailed reference viewed: 21 (0 ULiège)
See detailNovel promising way to synthesize non-isocyanate polyurethanes (NIPUs) for innovative coating applications against corrosion protection of metal surfaces
Panchireddy, Satyannarayana ULiege; Grignard, Bruno ULiege; Thomassin, Jean-Michel ULiege et al

Poster (2016, October 13)

Polyurethanes (PUs) are one of the most widely used polymeric materials with applications in automotive, as sealants, adhesives, decorative, aircraft topcoats, for coatings or as foams for thermal and/or ... [more ▼]

Polyurethanes (PUs) are one of the most widely used polymeric materials with applications in automotive, as sealants, adhesives, decorative, aircraft topcoats, for coatings or as foams for thermal and/or acoustic insulation. Industrially, PUs are produced by copolymerization between polyols and polyisocyanates. Because of the toxicity issues related to the use of isocyanates, there is a need to develop greener and safer route to produce such polymers. This study reports on the synthesis of new sustainable isocyanates-free PU (NIPU) coatings for aluminium anticorrosion. In a first step, bio- and CO2-sourced cyclic carbonates monomers were synthesized by coupling of epoxydized vegetable oils with CO2 using a new efficient bicomponent organocatalyst. In second step formulations including cyclic carbonates/amines/additives were optimized to produce highly adhesive cross-linked NIPU coatings that show good resistance against solvents and long-term barriers preventing the corrosion of the aluminiumsubstrate [less ▲]

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See detailA Safe and Reliable Continuous-Flow System towards Methylphenidate Hydrochloride
Gerardy, Romaric ULiege; Monbaliu, Jean-Christophe ULiege

Poster (2016, October 13)

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous ... [more ▼]

The batch-to-flow translation of a carbene-based strategy for the diastereoselective preparation of the active pharmaceutical ingredient threo-methylphenidate hydrochloride is presented. The continuous-flow system implies the generation of an explosive organic azide, its consumption in a diazo transfer reaction, an intermediate purification step followed by a Rh(II)-catalyzed carbene C-H insertion reaction that produces threo-enriched N-Boc-methylphenidate. Boc-deprotection is carried out off-line and provides methylphenidate hydrochloride in 19% isolated yield (3 steps fully telescoped) or 38% isolated yield (sequential). [less ▲]

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See detailImplication of AurA kinase in GBM cells chemotaxis in response to the production of CXCL12 in the subventricular zones
Willems, Estelle ULiege; Dedobbeleer, Matthias ULiege; LOMBARD, Arnaud ULiege et al

Poster (2016, October 13)

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the ... [more ▼]

Despite great improvement in standard therapies (i.e. surgery, radiotherapy and chemotherapy) of glioblastoma (GBM), the median survival rate is 15 months due to patient relapses. A major advance in the understanding of GBM recurrences has been the identification of GBM-initiating cells (GIC). GIC are thought to be deeply involved in GBM recurrences. Our lab designed a mouse model by grafting human GBM cells in the striatum. After the graft, we observed that tumors develop in the mouse striatum and that GIC specifically invade the subventricular zones (SVZ). SVZ are stem cells niches crucial for adult neurogenesis which seems particularly propitious for gliomagenesis since they are abundant in growth factors and permissive to proliferation. We therefore looked for soluble factors secreted by the SVZ environment and demonstrated that the local production of the CXCL12 chemokine in the SVZ is responsible for the GIC-directed migration. In this work, we aim to study the role GBM therapeutic resistance associated with the invasion of the SVZ. In this work, we identified a new actor of the CXCL12 pathway by the phosphoproteome analysis of U87MG cells stimulated with CXCL12: the mitotic kinase Aurora A (AurA) whose activity seems crucial for the CXCL12-dependent chemotaxis of GBM cells [less ▲]

Detailed reference viewed: 31 (5 ULiège)
See detailSMFS on synthetic helical foldamers
Devaux, Floriane ULiege; Li, Xuesong; Huc, Ivan et al

Poster (2016, October 13)

Detailed reference viewed: 21 (0 ULiège)