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See detailEstetrol Attenuates Neonatal Hypoxic-Ischemic Encephalopathy: Preclinical Studies
Tskitishvili, Ekaterine ULg; Nisolle, Michelle ULg; Noël, Agnès ULg et al

Poster (2016, June 17)

Brain hypoxia and ischemia due to systemic hypoxemia and reduced cerebral blood flow (CBF) are the primary causes of neonatal hypoxic-ischemic encephalopathy (HIE) accompanied by gray and white matter ... [more ▼]

Brain hypoxia and ischemia due to systemic hypoxemia and reduced cerebral blood flow (CBF) are the primary causes of neonatal hypoxic-ischemic encephalopathy (HIE) accompanied by gray and white matter injuries occurring in neonates. Perinatal HIE still remains a challenge in perinatal medicine. About 20% of affected newborns die in the postnatal period, and an additional 25% will sustain childhood disabilities. So far no medical treatment provides important neuroprotection against HIE. Studies of new neuroprotective agents in animal models of HIE may have importance for the development of new compounds and treatment strategies for this pathological condition. Estetrol (E4) is a recently described estrogen with four hydroxyl-groups that is synthesized exclusively during pregnancy by the human fetal liver. It has important antioxidative activity. To study the neuroprotective and therapeutic effects of E4 in vivo neonatal HIE model of 7-day-old newborn rat pups was used. Rat pups body temperatures were examined along with their body and brain weights. Brains were studied at the level of the hippocampus and cortex. Intact cell counting and expressions of markers for neuronal cell viability (microtubule-associated protein-2 (MAP-2)), neurogenesis (doublecortin (DCX)) and angiogenesis (vascular-endothelial growth factor (VEGF)) were evaluated by histo- and immunohistochemistry. The serum levels of brain damage markers (S100B and glial fibrillary acidic protein (GFAP)) were measured by ELISA. Our results demonstrate for the first time that E4 has a significant neuroprotective and therapeutic effects. It decreases the early gray matter loss and promotes neuro- and angiogenesis in vivo. Estetrol treatment has no effects on body weight, brain weight or body temperature. Taken together, E4 might become an important safe and physiological substance to treat neonatal HIE. [less ▲]

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See detailSequence-based association analysis identifies coding and non-coding variants in HFM1, MLH3, MSH4, MSH5, RNF212 and RNF212B with large effects on male and female recombination rate in cattle
Kadri, Naveen Kumar ULg; Harland, Chad ULg; Faux, Pierre ULg et al

Poster (2016, June 15)

We herein study genetic recombination in three dairy cattle populations from France, New-Zealand and the Netherlands. We identify 2,395,177 crossover (CO) events in sperm cells transmitted by 2,940 sires ... [more ▼]

We herein study genetic recombination in three dairy cattle populations from France, New-Zealand and the Netherlands. We identify 2,395,177 crossover (CO) events in sperm cells transmitted by 2,940 sires to 94,516 offspring, and 579,996 CO events in oocytes transmitted by 11,461 cows to 25,332 offspring. When measured in identical family structures, the average number of CO in males (23.3) was found to be larger than in females (21.4). The heritability of global recombination rate (GRR) was estimated at 0.13 in males and 0.08 in females. The genetic correlation was equal to 0.66, indicating that shared variants are influencing GRR in both genders. Haplotype-based genome-wide association studies revealed seven genome-wide significant QTL. Variants identified by next-generating sequencing in 5 Mb windows encompassing the QTL peaks were imputed in order to perform a sequence-based association analysis. For four QTLs, we identified missense mutations in genes known to be involved in meiotic recombination among the most significantly associated variants. Most of the identified mutations had significant effects in both genders with three of them accounting each for approximately 10% of the genetic variance in males (the allelic substitution effect being approximately equal to one additional CO per genome). Thus, a large fraction of the genetic variance is associated with missense mutations in genes known to be involved in meiotic recombination. Our results are very different from reports of recombination in other species. For instance, in human, recombination rate is higher in females, distinct variants affect recombination rate in males and females, and the genetic correlation is close to 0, whereas in cattle, we observed a higher recombination rate in males controlled by shared variants effective in both sexes. [less ▲]

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See detailWater Vapor Permeability of edible packaging based on native starch from improved cassava (Manihot esculenta Crantz) variety in Ivory Coast
Adjouman, Yao Désiré; Nindjin, C.; Tetchi, F.A. et al

Poster (2016, June 13)

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See detailLignin as a bio-based flame retardant additive: Pretreatments overview
Istasse, Thibaut ULg; Jacquet, Nicolas ULg; Richel, Aurore ULg

Poster (2016, June 12)

Lignin is a cross-linked phenol polymers from plant cells wall and is the third most abundant organic material after cellulose and hemicelluloses. This substance was studied as a flame retardant for ... [more ▼]

Lignin is a cross-linked phenol polymers from plant cells wall and is the third most abundant organic material after cellulose and hemicelluloses. This substance was studied as a flame retardant for various polymers and represents an interesting opportunity to create more eco-friendly and safer plastics and resins. This poster explains one potential technology to extract lignin: the organosolv treatment. [less ▲]

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See detailWater familiarization testing battery adapted for young children
Vandermeulen, Mary ULg; Schietecatte, Delphine; Delvaux, Anne ULg et al

Poster (2016, June 11)

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See detailHOME BLOOD PRESSURE IN KIDNEY TRANSPLANT RECIPIENTS (Ktr)-VALIDITY OF DIFFERENT SCHEDULES OF SELF-MONITORING
Saint-Remy, Annie ULg; WEEKERS, Laurent ULg; BONVOISIN, Catherine ULg et al

Poster (2016, June 11)

Office blood pressure (OBP) coupled with 24-h ambulatory monitoring (24-h ABPM) or home self-monitoring (HBPM) allow a more accurate assessment of BP control in treated hypertensive patients and ... [more ▼]

Office blood pressure (OBP) coupled with 24-h ambulatory monitoring (24-h ABPM) or home self-monitoring (HBPM) allow a more accurate assessment of BP control in treated hypertensive patients and identification of different phenotypes of BP. ESH/ESC guidelines (2013) recommended 7 days of home measurements (3 days at least) but that duration is questioned. The present study examined if we can reduce, and to what extent, the 7-days schedule for home measurements in treated hypertensive kidney transplant recipients (ktr) while keeping a reliable assessment of their BP status? [less ▲]

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See detailVers une approche multidimensionnelle de la procrastination.
Rebetez, Marie; Rochat, Lucien; Barsics, Catherine ULg et al

Poster (2016, June 10)

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See detailThe Computer in German Literature at the Turn of the Millennium
Dupont, Bruno ULg

Poster (2016, June 09)

This poster shows how the representation of the computer in German literature since 1990 changes the very form and content of literary works. The social importance of the computer challenges the texts on ... [more ▼]

This poster shows how the representation of the computer in German literature since 1990 changes the very form and content of literary works. The social importance of the computer challenges the texts on three levels: their topical organisation, the language used for the transcription of the new reality, and the ideological positioning on new media. [less ▲]

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See detailFunctional analysis of PEBP genes in root chicory (Cichorium intybus)
Saintmard, Nicolas ULg; Tocquin, Pierre ULg; Périlleux, Claire ULg

Poster (2016, June 09)

Manipulating plant architecture is key to increase crop yield. In this perspective, basic knowledge on the molecular mechanisms regulating plant growth and development will be fundamental for the future ... [more ▼]

Manipulating plant architecture is key to increase crop yield. In this perspective, basic knowledge on the molecular mechanisms regulating plant growth and development will be fundamental for the future of agriculture. Recent progress indicates that a family of plant genes, homologous to POSPHADITYLETHANOLAMINE-BINDING PROTEINS (PEBP) in other eukaryotes, plays critical roles in controlling shoot development traits such as branching, flowering, bud dormancy or tuberization. Very little is known however on the functions of these genes in root development. The current research will attempt to answer this question in the case of Cichorium intybus, a biannual plant cultivated for the extraction of inulin (a polymer of fructose) from its root. Functional analyses will include expression kinetics, complementation tests in Arabidopsis mutants and creation of PEBP- overexpressing or silencing chicory plants. The preliminary steps of the project are the identification of PEBP genes in chicory by in silico analyses, the determination of the time of the storage root initiation and the establishment of a regeneration protocol for the genetic transformation. [less ▲]

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See detailTowards a Digital Philology. Creation, Spread and Apprehension of Knowledge through Digital Scholarly Platforms in Humanities
Mayeur, Ingrid ULg

Poster (2016, June 09)

This contribution aims to present and discuss my thesis project. I plan to study the concrete conditions of production, diffusion and reception of knowledges on digital scholarly platforms in Humanities ... [more ▼]

This contribution aims to present and discuss my thesis project. I plan to study the concrete conditions of production, diffusion and reception of knowledges on digital scholarly platforms in Humanities. This work will especially focus on the four plateforms of the portal OpenEdition: OpenEdition Books, Calenda, Hypothèses and Revues.org. The main problematic is: what are the uses of those platforms, and in what concrete way are they influencing the modes of scientific communication? To start with, I need to highlight the frameworks that structure the digital scholarly texts, that Jeanneret named ‘architexts’ (Davallon & al. 2003, Jeanneret 2014). Such frameworks contribute to configure the digital texts (e. g. with hypertext, flows aggregation), and their uses. Three aspects will then be taken into consideration in my analysis: (I) the articulation of temporal stratums within the plateform: how do archival materials deal with actualities (scientific monitoring, work in progress as grey literature)? What means of contextualization (Treleani 2013) or editorialization (Vitali Rosati 2016) could be given when an old document is used again (e.g. in “epi-revues” that collect scientific papers) ? (II) What are the genres and formats conveyed on the plateform, proper to the Web as blog post, commentary, etc., or still used in other medias as scientific article, advert, etc. (Maingueneau 2013)? (III) Who are the users of those plateforms (researchers, groups, professionals, etc.)? How do they deal with their digital identities? How can they appropriate and diffusing knowledges, by citing, reusing and discussing? How are emerging new ways for research in Humanities, through collective negociation or evaluation, interdisciplinarity – such actions that could be promoted by the technical device of the plateform? This last point will be treated by a philological approach concerning paratexts and quotations (Genette 1982, Compagnon 1979). Other indicators (users’ itineraries, interactions between members, etc.) could also be used, and treated with network’s visualization tool. In summary, this research project aims to explore the means of a digital philology (Crane, Bamann and Jonesin Schreibman 2008; Rastier 2011), not only based on making traditional philology with digital tools (i.e. semantic enrichment of antics or medieval texts), but also on a critical approach of the digital textuality and on an active as well as reflexive contribution from the users in building knowledges. References Davallon, Jean, Marie Després-Lonnet, Yves Jeanneret, Joëlle Le Marec, and Emmanuël Souchier. 2003. Lire, écrire, récrire : Objets, signes et pratiques des médias informatisés. Études et recherche. Paris: Éditions de la Bibliothèque publique d’information. Compagnon, Antoine. 1979. La Seconde main ou le Travail de la citation. Paris: Seuil. Genette, Gérard. 1982. Palimpsestes: La littérature au second degré. Paris: Seuil. Maingueneau, Dominique. 2013. « Genres de discours et web : existe-t-il des genres web ? » In Manuel d’analyse du web en Sciences Humaines et Sociales, 74 93. Paris: Armand Colin. Mounier, Pierre, and Marin Dacos. 2010. L’édition électronique. Paris: La Découverte. Rastier, François. 2011. La mesure et le grain. Sémantique de corpus. Paris: Honoré Champion. Treleani, Matteo. 2014. Mémoires audiovisuelles: les archives en ligne ont-elles un sens? Montréal: PUM. Vitali-Rosati, Marcello. 2016. « What is editorialization? » Sens public, janvier. http://www.sens-public.org/article1059.html, consulté le 10 février 2016. Crane Gregory, David Bamman, and Alison Jonesin Schreibman, “ePhilology: When the Books Talk to Their Readers” in Susan, et Ray Siemens, éd. 2008. Companion to Digital Literary Studies. Oxford: Blackwell Publishing Professional. URL : http://www.digitalhumanities.org/companion/view?docId=blackwell/9781405148641/9781405148641.xml&chunk.id=ss1-4-1&toc.depth=1&toc.id=ss1-4-1&brand=9781405148641_brand. Consulted on 10 fev. 2016.   [less ▲]

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See detailMALDI-imaging guided microproteomics workflow for biomarker discovery of intra-tumor heterogeneity
Alberts, Deborah ULg; Longuespée, Rémi ULg; Smargiasso, Nicolas ULg et al

Poster (2016, June 09)

Introduction A single tumoral tissue can bear phenotypically different cell populations. This phenomenon called intra-tumor heterogeneity can lead to differential behaviors regarding metastasis seeding ... [more ▼]

Introduction A single tumoral tissue can bear phenotypically different cell populations. This phenomenon called intra-tumor heterogeneity can lead to differential behaviors regarding metastasis seeding and therapy resistance [Zardavas et al., Nature Rev. Clin. Onc. 2015]. MALDI imaging has proven its efficiency for revealing hidden molecular features offering an insight into distinct cellular regions based on their molecular content. Further, proteomics applied to these regions could allow depicting the molecular context associated to particular cells groups and enable the collection of qualitative, quantitative and spatial information for each protein. Methods Breast cancer Formalin Fixed and Paraffin Embedded tissues, from patients whose outcome had been recorded over a period of 10 years, were provided by the department of Pathology of University of Liège. After Citric Acid Antigen Retrieval and trypsin digestion, images were obtained by MALDI-TOF/TOF-MS (Bruker, Germany). From the obtained datasets, segmentation and analytical data analysis were applied using SCiLS (Bruker, Germany) and the cloud software Multimaging (ImaBiotech, France). Small tissue areas were obtained by laser microdissection (LEICA LMD 700, Germany), upon which a combination of chemical processes was applied to ensure optimal protein antigen retrieval, extraction and digestion. Finally, the tissue pieces obtained were analyzed by LC-MS/MS using UPLC Waters Nanoacquity and Thermo Q-Exactive instruments. Preliminary data Based on mathematical calculations for the MALDI imaging datasets of the breast cancer FFPE tissues, Regions Of Interest (ROIs) were detected in a single tumor, revealing intra-tumoral heterogeneity, which can be correlated to the level of aggressiveness of the affliction and to the final prognosis of the patient. We aimed to compare the proteomic profiles of each of the small ROIs. Until today, proteomics applied to tissues composed by a restricted number of cells is quite tedious due to possible tissue losses during their handling. Recently, Longuespée [Longuespée et al., Methods 2015] published a method in order to retrieve the identification of 1400 proteins from microdissected tissue pieces containing only 2700 cells. This whole procedure allowed us to identify a panel of protein that characterizes tissue heterogeneity within a single tumor. This proves the applicability of the combination of MALDI imaging for the discovery of intra-tumoral heterogeneity without a priori, on a mathematical basis, and classical proteomics applied on laser-microdissected tissue samples of very restricted areas. This method will now be applied to several MALDI datasets in order to retrieve commune ROIs and to associate their presence with the information of each patient, such as their prognosis. Those ROIs will then be microdissected and subjected to microproteomic methods that will allow us to retrieve the extensive molecular context associated to bad patient prognosis and/or therapy resistance. The possibility to identify protein/peptide markers will have the power to predict the outcome of the breast cancer patient at the beginning of their treatment, and thus, improve the clinical care for the benefit of the patients. Novel aspect The workflow combines the unique advantages of MALDI imaging for de novo molecular features characterization and LMD-based microproteomics. [less ▲]

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See detailCharacterization of FRD3, a iron and zinc homeostasis actor in Arabidopsis relatives
Scheepers, Maxime ULg; Charlier, Jean-Benoit; Spielmann, Julien ULg et al

Poster (2016, June 09)

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See detailAg/SiO2 and Cu/SiO2 cogelled xerogel catalysts for benzene combustion and 2-butanol deshydrogenation
Mahy, Julien ULg; Claude, Vincent ULg; Tasseroul, Ludivine ULg et al

Poster (2016, June 08)

Tars are recognized as major impurities when biomass is gazified into Syngas (mixture CO + H2) or when the methane reforming was produced from sustainaible reagents [1]. Therefore, fundamental knowledge ... [more ▼]

Tars are recognized as major impurities when biomass is gazified into Syngas (mixture CO + H2) or when the methane reforming was produced from sustainaible reagents [1]. Therefore, fundamental knowledge of catalytic oxidation and deshydrogenation mechanisms can be valuable to develop effective methods to control syngas or methane pollution. A very important concern about cogelled catalysts is the accessibility of the active centers. Because the silver and copper is located inside silica particles, there is a risk that it may not be accessible. In the case of benzene oxidation, it was observed that the specific activity (mol s-1 g-1Ag) of Ag/SiO2 and Cu/SiO2 catalysts increases when the silver or copper loading decreases (Fig. 1). In fact, visual observations by TEM showed that in samples Ag0.25, Ag0.45, Ag1.05 and Cu1.00, there are not silver or copper particles located on silica particles external surface and then the metal dispersion values are greater in these samples. So, it is the proof that Ag and Cu particles located inside the silica particles are accessible for benzene in this catalytic system. In the case of 2-butanol dehydrogenation, the specific activity of Cu/SiO2 catalysts increases when the copper loading increases or when the metal dispersion values decreases (Fig. 2). However, in Cu1.00 sample, copper particles inside silica particles only are observed by TEM. It seems that these metallic particles are accessible for 2-butanol in this catalytic system. [less ▲]

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See detail50Hz EMF and public health policy: The involvement of the Belgian BioElectroMagnetics Group
Ledent, Maryse ULg; Maes, Annemarie; De Ridder, Maurits et al

Poster (2016, June 07)

The BBEMG website (www.bbemg.be) aims to present the results of the research teams of the Belgian BioElectroMagnetics Group, to inform about electric and magnetic fields and their potential health effects ... [more ▼]

The BBEMG website (www.bbemg.be) aims to present the results of the research teams of the Belgian BioElectroMagnetics Group, to inform about electric and magnetic fields and their potential health effects, and to become a centre of documentation and education able to provide understandable and correct information regarding 50 Hz electric and magnetic fields to the general public, physicians, scientists, public officials... This paper is focusing on the involvement of the BBEMG through its website in the Belgian public policy, its identified goals and how it is implemented and evaluated. Challenges will also be analysed. [less ▲]

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See detailFrom brownfields to greenfields: rural techniques to recover injured areas
Baldin, Elisa ULg

Poster (2016, June 07)

The de-industrialization process, which has interested for years Vallonia’s region, sustains the debate on territories reuse, where the labour model which had generated them, ceases to exist. Particularly ... [more ▼]

The de-industrialization process, which has interested for years Vallonia’s region, sustains the debate on territories reuse, where the labour model which had generated them, ceases to exist. Particularly in the province of Liège, places reveal how the arrival of industrial economy has transformed identities and structures of the local rural landscape, turning it into a fragmented and hybrid territory. The presence of industry has caused the concentration of material and social capitals and imposed a model of life based on the augmentation of consumption and waste, breaking the pre-existing environmental and social balances linked to the rural economy system. The damaging effects linked to industrial production model, based on the intensive exploitation of resources, have generated a gap between territories and their inhabitants and the consequent escape towards “uncontaminated” lands, where new residential zones extended, exporting “urban” way of life. The closure of steel industries left a large heritage of environmental degradation: a constellation of “derelict lands” specially along the main rivers, from the Meuse to the Sambre. These industries, after extracting, transforming, setting down materials, have stopped their operations and have left huge inaccessible areas, ruins and waste, fading the idea of a possible inhabited landscape, because of the compromised conditions of lands. In these discontinuous and “polycentric” territories the reconfiguration of the rural altered system, starting from recycle and remediation interventions through the reintroduction of elements and dynamics belonging to the agricultural system, has a double value. At a first level to lead an ecologic reactivation process, where time and “scenarios” correspond to time and answers given by cultivations. Furthermore, this new way of approaching territory, considering new access and uses, promotes a gradual re-appropriation of the lands by the citizens, who discover new way of life. The act of “cultivating”, interpreted as “taking care of something, recovering it or not letting degrade it” (V. Tuzzi), refers to a condition of new rurality, where local development is founded on the respect of environmental and landscape typical characters, reinterpreted as resources to reactivate cycles of life: from the reconstruction of ecosystems, to the activation of new production chains and to the discovery of shared green spaces. The reclamation project of the HF6 blast furnace area, in Seraing, represents a study case, where the landscape approach leads the re-writing of parts of the city, starting from the open spaces, which become green surfaces to mend the pre-existent landscape weave. Through phytoremediation techniques and monitoring activities on spontaneous vegetal recolonization, the land is prepared to a gradual reuse process. Environmental engineering, agronomy and landscape planning contribute to a new “agricultural landscape”, intended as a “perennial in progress fact rather than a defined fact” (P.Bevilacqua), reimporting a condition of alter rurality, based on the activation of a virtuous production chain, on the protection of environment and landscape and on the balance in territorial development. [less ▲]

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See detailMOISTURE CONTENT DETERMINATION OF FREEZE-DRIED PRODUCTS BY NEAR-INFRARED SPECTROSCOPY: A CASE STUDY FOR UNIVERSAL REGRESSION MODEL
Clavaud, Matthieu ULg; Roggo, Yves; Degardin, Klara et al

Poster (2016, June 06)

Karl Fischer titration is the reference method for moisture content determination in the pharmaceutical industry. Near-infrared spectroscopy is considered the most suitable alternative technique. Indeed ... [more ▼]

Karl Fischer titration is the reference method for moisture content determination in the pharmaceutical industry. Near-infrared spectroscopy is considered the most suitable alternative technique. Indeed, NIRS is a safe and fast method which does not require sample preparation. Nevertheless, the development and validation phases are time-consuming. In addition, the NIRS methods presented so far were mostly product specific. The main objective of this study is to highlight that an universal calibration model can be validated for several freeze-dried products in order to speed up the validation time. This objective was led in two steps. A universal model was first evaluated. A calibration set and a validation set were built up with three freeze-dried products. An antibody drug conjugate, a large molecule and a small molecule all packed in sealed vials were used to introduce more variability. Regression methods were then compared in order to optimize the prediction values. [less ▲]

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See detailOn the α-polarization of bacterial suspensions: SIP measurements on four bacterial strains
Pilawski, Tamara ULg; Tappe, Wolfgang; Zimmermann, Egon et al

Poster (2016, June 06)

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See detailModélisation d'une climatisation automobile multi-évaporateurs
Gillet, Thomas ULg; Rullière, Romuald; Haberschill, Philippe et al

Poster (2016, June 02)

La modélisation d’une climatisation automobile multi-évaporateurs, composées de deux évaporateurs et d’un refroidisseur de fluide secondaire, a été réalisée à l’aide du logiciel LMS Imagine.Lab Amesim® 1D ... [more ▼]

La modélisation d’une climatisation automobile multi-évaporateurs, composées de deux évaporateurs et d’un refroidisseur de fluide secondaire, a été réalisée à l’aide du logiciel LMS Imagine.Lab Amesim® 1D. Dans le but d’étudier le comportement de la boucle, des perturbations sous la forme d’échelon ont été simulées sur un point de fonctionnement à moyenne et haute charge. L’impact de ces perturbations sur la stabilité de la température d’air soufflé est analysé pour deux types de détendeur. Les premiers résultats montrent que les détendeurs thermostatiques peuvent engendrer des instabilités. [less ▲]

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See detailScreening of mahoran plants for cosmetic applications.
Saive, Matthew ULg; Frederich, Michel ULg; Fauconnier, Marie-Laure ULg

Poster (2016, June 01)

This study’s main aim is to identify plant species showing anti-oxidant, anti-inflammatory and skin whitening properties. A selection of 89 samples from previous infield studies on the traditional uses of ... [more ▼]

This study’s main aim is to identify plant species showing anti-oxidant, anti-inflammatory and skin whitening properties. A selection of 89 samples from previous infield studies on the traditional uses of plants in Mayotte were analyzed. The anti-oxidant activity was assessed using the α, α-diphenyl-β-picrylhydrazyl (DPPH) method developed by Blois [1]. The anti-inflammatory activity and the skin whitening activity were both assessed through the study of inhibition kinetics from specific enzymes: respectively, lipoxygenase (EC 1.13.11.12) due to its important role in the leukotriene pathway and tyrosinase (EC. 1.14.18.1) as it takes part in the melanogenesis pathway. The IC 50 value for each sample and for each activity was obtained using UV/Vis spectrophotometric technics. These IC 50 were then compared with the ones obtained from known molecules found in the literature: namely, (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (IC50 = 4,388 µM) for the anti-oxidant activity, nordihydroguaiaretic acid (IC50 = 3,5µM) for the anti-inflammatory activity and kojic acid (IC50 = 722 µM) for the anti-tyrosinase activity. The most effective anti-oxidant activity was observed in fresh leaves from Acalypha wilkesiana Müll.Arg. (IC50 = 0,381 g/L) and Leea guineensis G. Don. (IC50 = 0,225 g/L) as well as in dried roots from Litchi chinensis Sonn. (IC50 = 0,346 g/L). All results are expressed in grams of fresh matter. According to the preliminary tests for the-anti-inflammatory and skin whitening activities, the crude extracts from Kalanchoe pinnata Lam. Pers., Litchi chinensis Sonn., Zingiber zerumbet (L.) Sm., Leea guineensis G. Don. and Paullinia pinnata L. are likely to show promising activities. [less ▲]

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See detailPhysiological response to training and competition in 1-star to 4-star eventing horses
KIRSCH, Katharina; Düe, Michael; Holzhausen, Hermann et al

Poster (2016, June)

Abstract 034 – Physiological response to training and competition in 1-star to 4-star eventing horses – Post Review Authors’ names and affiliations Author 1: Katharina Kirsch* (German Olympic Committee ... [more ▼]

Abstract 034 – Physiological response to training and competition in 1-star to 4-star eventing horses – Post Review Authors’ names and affiliations Author 1: Katharina Kirsch* (German Olympic Committee for Equestrian Sports, Germany and PhD Student, University of Liège, Belgium) Author 2: Michael Düe (German Equestrian Federation, Germany) Author 3: Hermann Holzhausen (Olympic Support Center Westphalia – Warendorf, Germany) Author 4: Stephanie Horstmann (German Olympic Committee for Equestrian Sports, Germany) Author 5: Markus Scharmann (German Equestrian Federation, Germany) Author 6: Charlotte Sandersen (University of Liège, Belgium) *Address of the presenting author: Katharina Kirsch, Freiherr-von-Langen-Str. 15, 48231 Warendorf, GERMANY E-mail of the presenting author: kkirsch@fn-dokr.de Abstract Physiological response to training and competition in 1-star to 4-star eventing horses To prepare horses for the requirements of international eventing competitions and simultaneously maintain their health and welfare, appropriate training is mandatory. Specific training and competition management necessitates information about exercise intensities and fitness, which are usually assessed by standardized exercise tests. Our purpose was to identify parameters whose measurement can be largely integrated in the daily training and competition routine and which provide information on fitness and adequacy of applied training. Further, we evaluated training and competition intensities of eventing horses, based on data generated by a proportionally large group of horses over several years. Data of 187 horses from 1-star to 4-star level over a time period of six years were collected during 410 training sessions and 916 Cross Country rides, including measurement of covered distance and speed (GPS, Fidelak EquiPILOT), continuously recording of heart rate (HR) (Polar T52H) and determination of blood lactate concentrations (BLC) (Dr. Lange photometer). Characteristics of the track, altitude profile, ambient temperature and humidity were recorded at each session. The results of the one-way ANOVA indicated a significant effect of competition level on HR (F3,373=23.29; p<0.001) and BLC (F3,763=46.12; p<0.001). Under competition conditions, HR and BLC increased from 1-star to 3-star level (1-star: HR=194±9 bpm, BLC=7.7±5.6 mmol/l; 2-star: HR=198±9 bpm, BLC=9.6±6.2 mmol/l; 3-star: HR=205±10, BLC=15.6±9.8 mmol/l; 4-star: HR=207±2 bpm, BLC=12.4±9.3 mmol/l), while under training conditions, they decreased with increasing competition level (1-star: HR=184±29 bpm; BLC=18.9±11.1 mmol/l; 2-star: HR=182±19 bpm; BLC=12.1±9.0; 3-star: HR=174±25 bpm; BLC=6.9±7.1 mmol/l; 4-star: HR=161±24 bpm, BLC=4.0±5.6 mmol/l). On 1-/2-star level, BLCs after training exceeded those after competition. Reverse applied for 3-/4-star level. On 3-star level, the percentage of HRs above 200 bpm during Cross Country was considerably greater than on lower levels (1-star: 33%; 2-star: 54%; 3-star: 94%). The competition format (CCI/CIC) had no significant effect on the progress of HR. The altitude profile however, had a significant effect on HR (F1,201=26.72, p<0.001) and BLC (F1,89=25.56; p<0.001). Evaluation of physiological response to training and competition through implementation of measurement technology allows an assessment of the different impacts on exercise intensities and should be more commonly used to assess appropriateness of training and competition management in eventing horses. It should be further investigated if the detected discrepancies in the response to training and competition between levels are a result of different fitness ore varying training strategies. LP To meet the demands of international Eventing competitions, a systematic training of horses is necessary. Close monitoring of physiological response to exercise during training and competition can help to improve training. Keywords Exercise physiology; training; eventing; heart rate; lactate. [less ▲]

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See detailSome results about diametral dimensions
Demeulenaere, Loïc ULg

Poster (2016, June)

The diametral dimension is an important topological invariant, especially in the context of Köthe sequence spaces. This poster presents some results concerning the equality of the diametral dimension with ... [more ▼]

The diametral dimension is an important topological invariant, especially in the context of Köthe sequence spaces. This poster presents some results concerning the equality of the diametral dimension with one of its variants. It is based on a joint work with Françoise Bastin, Leonhard Frerick, and Jochen Wengenroth. Firstly, it gives sufficient conditions to have the equality between the two diametral dimensions for a Fréchet space. Secondly, it provides some examples of spaces verifying these conditions. Finally, it gives a family of Schwartz - or even nuclear - (non metrizable) locally convex spaces for which the two diametral dimensions are different. [less ▲]

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See detailAssessment of fine scale population genetic diversity and regeneration in Congo basin logged forests
Evrard, Quentin ULg; Daïnou, Kasso ULg; Hardy, Olivier et al

Poster (2016, June)

Background In the Congo Basin most of the light-demanding timber tree species display a deficit of natural regeneration which is a major handicap for sustainable production and certification. Whilst the ... [more ▼]

Background In the Congo Basin most of the light-demanding timber tree species display a deficit of natural regeneration which is a major handicap for sustainable production and certification. Whilst the majority of scientists investigate abiotic and biotic factors explaining that pattern, we hypothesize that tree population density or individual spatial isolation may also affect the tree fitness through inbreeding. In this study, we integrate ecological and genetic approaches to characterize the regeneration potential of a set of priority timber species by (i) estimating pollen dispersal distances at various tree population densities, and (ii) evaluating the impact of increasing spatial isolation on mating characteristics and tree fitness. The ultimate goal is the proposal of minimum population density that prevents inbreeding consequences. Method This ongoing study focuses on 10 timber species (Pericopsis elata, Milicia excelsa, Baillonella toxisperma, Entandrophragma cylindricum, E. utile, E. angolense, E. candollei, Afzelia bipindensis, Erythrophleum suaveloens, Terminalia superba). The data collection was carried out in the logging concession granted to Pallisco in Cameroon. We established two 400-ha plots, where all individuals (DBH > 10 cm) of the target species were inventoried and mapped. A sample of leave or cambium was collected for each of these individuals, as well as for seedlings to characterize patterns of gene flow using genetic tools (nuclear microsatellites). Dispersal agents were identified by direct observations and camera traps. Germination success was characterized in nursery for seeds collected on trees under an increasing isolation gradient. Results Main dispersal agents (wind, bat, rodent) and predators (rodent) were identified for all the species. The gene flow and germination data is still being analyzed and the main results will be presented in the poster. Conclusion Our data will allow characterizing the reproductive biology of a set of important timber species from the Congo basin. These information will strengthen sustainable forest management and the application of certification by adjusting harvesting norms through the use of scientifically-relevant data. In particular, we will tentatively define a maximum distance to be maintained between two adults to allow a qualitative reproduction. [less ▲]

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See detailEffects of protein source and cooking procedure on intestinal microbiota and on fermentation end-products in rats
POELAERT, Christine ULg; Despret, Xavier; Portetelle, Daniel ULg et al

Poster (2016, June)

Animal and plant proteins are major proteins sources in the human diet. After their enzymatic degradation in the upper gastro-intestinal tract, the undigested fraction of these proteins is available for ... [more ▼]

Animal and plant proteins are major proteins sources in the human diet. After their enzymatic degradation in the upper gastro-intestinal tract, the undigested fraction of these proteins is available for fermentation by the microbiota of the large intestine leading to the production of short-chain fatty acids (SCFA), branched-chain fatty acids (BCFA), ammonia, biogenic amines, sulphur metabolites, phenols and indoles. As some of these compounds have genotoxic and cytotoxic effects, protein fermentation is considered as detrimental to the host’s epithelial health. BCFA are usually used as a marker of intestinal protein fermentation. We studied in vivo the impact of proteins from animal and plant origin, raw or after a cooking procedure, on the composition of gut microbiota and on fermentation end-products. Weanling rats were used as models of the human gut microbiota. Eight experimental diets were formulated with beef meat (Longissimus dorsi), chicken meat (Pectoralis major), white pea beans (Phaseolus vulgaris), soybeans (Glycine max), used raw and cooked, as sole source of protein in the diet. One casein diet was used as control. All diets, formulated to contain 15% of raw protein, were given to seven rats for four weeks. After euthanasia, caecal contents were collected. Pyrosequencing analyses (Roche 454 GS Junior Genome Sequencer) were performed to study the microbial composition. SCFA and BCFA were measured using HPLC (Waters 2690). Microbial composition in the caecum is associated to the type of dietary protein and to the cooking procedure applied. The proportion of BCFA in the caecal content is mainly affected by the type of protein. So BCFA represent respectively 04-06% and 35-44% of total SCFA with diets based on plant and on animal proteins. In conclusion, both the type of protein and the cooking procedure could impact the gut microbiota in terms of composition and of fermentative capacity. [less ▲]

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See detailSpeech characteristic of French-speaking children with Dysarthria: Pilot study
MacLeod, andrea; Maillart, Christelle ULg; Ancelle, J. et al

Poster (2016, June)

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See detailImpact d'un programme d'activation comportementale sur l'humeur, le niveau d'engagement dans des activités, les ruminations et les biais attentionnels chez des étudiantes universitaires
Krings, Audrey ULg; Wagener, Aurélie ULg; Blairy, Sylvie ULg

Poster (2016, June)

A Behavioral activation program was proposed to undergraduated students with difficulties to deal with their mood. Six sessions of a Martell's program was proposed with two evaluations, before (T0) and ... [more ▼]

A Behavioral activation program was proposed to undergraduated students with difficulties to deal with their mood. Six sessions of a Martell's program was proposed with two evaluations, before (T0) and after (T1) the sessions. Different psychological processes were evaluated (behavioral activation, avoidance, rumination and attentional biases). Compared to the control group, a significal reduction was observed for abstract rumination, avoidance and a significant enhancement of selective attention to positive faces in the dot probe task was also observed. [less ▲]

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See detailMethodology to fish peptide ligands of nAChRs from Cone snail venoms by MALDI-TOF mass spectrometry
Echterbille, Julien ULg; Gilles, Nicolas; Araoz, Romulo et al

Poster (2016, June)

More than 50,000 of venomous species are currently indexed in the world. Each of their venom is composed of hundreds of toxins which potentially exhibit a high selectivity for membrane receptors such as ... [more ▼]

More than 50,000 of venomous species are currently indexed in the world. Each of their venom is composed of hundreds of toxins which potentially exhibit a high selectivity for membrane receptors such as GPCRs or ion channels. Among them, nAChRs are a target for drug discovery, primarily for treating central nervous system troubles. Therefore, the discovery of pharmacological tools and innovative drugs targeting nAChRs from animal venoms appears as an evidence. This study proposes the use a mass-spectrometry based methodology1 to discover new nAChRs ligands from cone snails venoms, and particularly -conotoxins (a-CTXs), known as potential antagonists of nAChRs2. in few words, Torpedo membranes, containing a high concentration of nAChRs, are incubated with BSA tryptic digests (>100 peptides) doped by small amounts of known a-CTXs. After two hours incubation, free (i.e. containing molecules remaining in solution) and bound (i.e. peptides bound to the membranes) fractions were analyzed with a MALDI-TOF/TOF mass spectrometer. The POC (positive and negative controls) as well as a real screening of Conus ermineus venom are presented. [less ▲]

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See detailSeasonal variation in human COGNITIVE brain responses
Meyer, Christelle; Muto, Vincenzo ULg; Jaspar, Mathieu ULg et al

Poster (2016, June)

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See detailCan serum progesterone be used to decide altrenogest supplementation discontinuation in problem pregnancies in mares?
Parrilla Hernandez, Sonia ULg; Delahaut, Philippe; Ponthier, Jérôme ULg et al

Poster (2016, June)

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation ... [more ▼]

Use of oral altrenogest as exogenous progesterone (P4) in mares in an effort to maintain high-risk pregnancies is a widespread practice. Treatment is sometimes initiated as early as 2 days after ovulation. Timing when it can safely be stopped is frequently uncertain and therefore, supplementation is often continued until 100-120 days, when the placental secretion of progestagens has taken over. The aim of this study was to determine if altrenogest interfered with progesterone assay and if timing of altrenogest administration discontinuation could be based on the blood concentration of P4. Data were obtained from 7 healthy standard breed mares in seasonal anoestrus and in dioestrus. Mares were considered anoestrus when no follicles larger than 15 mm in diameter or corpus luteum (CL) were found at 2 consequent ultrasound examinations 7 days apart. They were confirmed dioestrus based on an observed ovulation and the presence of a CL 2 days after. Mares were treated with 0.044 mg/kg of altrenogest (Regumate®, Intervet, Boxmeer, Netherlands) orally once per day during 12 days beginning at any time for the anestrus group and at day 2 after ovulation for the dioestrus group. Blood was collected at day 0, 6, 12, 18 right before altrenogest administration and the serum was stored at -20°C until analysis. P4 concentration was obtained by radioimmunoassay and altrenogest by liquid chromatography-mass spectrometry. Differences in P4 and in altrenogest concentrations between different sampling days were determined by Friedman non parametric test. Concentrations of altrenogest before treatment were almost not detectable (negative control). At day 6, they were significantly higher (p<0.05), and were back to basal, almost not detectable values at day 18, in both groups. P4 concentration was basal with values < 0.4 ng/ml all through the experiment in the anoestrus group. In the dioestrus group, P4 concentration was variable from one mare to another, but it was significantly higher in mid-dioestrus (day 6) as compared to the time of the impending next oestrus (day12). No cross-reaction between P4 and altrenogest assays was observed as illustrated by the fact that concentration of P4 during anoestrus remained basal with or without supplementation. Despite the treatment, the variation of P4 over time was as expected in both groups. Altrenogest was no longer detectable 6 days after the last administration. Further studies with larger numbers and sampling in dioestrus to compare P4 levels in treated and untreated mares to investigate the potential effect of altrenogest on endogenous P4 production by the CL are needed. However, our experiment shows that P4 levels assayed by RIA can be trusted to evaluate if the secondary CL’s have become functional and can thus maintain the pregnancy while oral altrenogest supplementation is discontinued. [less ▲]

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See detailDevelopment of improved preservation techniques for cyanobacterial and diatom strains in the BCCM collections
Crahay, Charlotte ULg; Chepurnova, Olga; Day, John G et al

Poster (2016, June)

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See detailTranscranial magnetic stimulation in sleep consciousness
Gosseries, Olivia ULg; Niemienen, Jaakko; Jason, Samaha et al

Poster (2016, June)

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See detailEducation of instructors of a physical activity program for cancer patients and survivors: planning and evaluation of a poster presentation session
Rompen, Jérôme ULg; Korycki, Marta ULg; Ortmans, Sabine et al

Poster (2016, June)

Introduction The Belgian Foundation against Cancer proposes an adapted physical activity (APA) program for cancer patients and survivors, called Raviva (http://www.cancer.be/raviva-bouger-pour-se-sentir ... [more ▼]

Introduction The Belgian Foundation against Cancer proposes an adapted physical activity (APA) program for cancer patients and survivors, called Raviva (http://www.cancer.be/raviva-bouger-pour-se-sentir-mieux).The activities are supervised by instructors with inconstant trainings and experiences. Contrary to other countries, Belgian APA instructors do not necessarily need to follow specific education programs such those proposed by the ACSM (Schmitz et al., 2010).We were involved in the implementation of a specific training day for in-service Raviva instructors. As it is the case in sport coaches’ education (Erickson et al., 2008), interacting with colleagues seemed to be an appropriate method to improve APA instructors’ skills. Therefore, interactive methods were proposed to be used in order to increase the participants’ interest. The aim of this paper was to describe and analyse a poster presentation session as well as to identify good practices. Methods As part of the training day, the poster presentation session consisted to create groups of 4 to 5 persons and to ask them to imagine and share solutions to specific problems linked with psycho-emotional aspects of the instruction process with Raviva groups. Three methods were used to collect the data: interviews of the organizers (n=4), a questionnaire for all trainees (n=35) and participant observation. Results Examples of good practices were listed and exchanged between the participants. All trainees considered that the poster presentation session was interesting and most of them (94%) reported that appropriate themes were discussed. Moreover, 88.2% of the participants mentioned that they were able to share their experiences. Three expressed negative opinions about the organization. These opinions were supported by all organizers as well as by the observation. Conclusions This educational tool seems to be interesting. However, organizers should plan enough time for such activity. Moreover, the time allocation between each part of the session should be better prepared and controlled. [less ▲]

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See detailAnalogical reasoning in children with Specific Language Impairment: Evidence from a scene analogy task
Krzemien, Magali ULg; Jemel, Boutheina; Maillart, Christelle ULg

Poster (2016, June)

Analogical reasoning development maintains a mutual influence with language acquisition: the use of relational labels helps to resolve analogical reasoning tasks (Christie & Gentner, 2014) while ... [more ▼]

Analogical reasoning development maintains a mutual influence with language acquisition: the use of relational labels helps to resolve analogical reasoning tasks (Christie & Gentner, 2014) while analogical reasoning enables the acquisition of new linguistic concepts or structures (Gentner & Namy, 2006). This link has driven some authors to examine the analogical reasoning ability of children with Specific Language Impairment. Those children have poorer analogical reasoning performance than their age-matched peers without language disorders (Leroy et al., 2012 ; Leroy et al., 2014). So, children with SLI seem to have an analogical reasoning weakness which could be linked to their language disorders. Thus, our goal here is to measure the ability of children with SLI to solve analogies, and to compare it to chronological age-matched but also to linguistic age-matched peers without language disorders. Our hypotheses here are the followings: children with SLI have weaker analogical reasoning competence compared to chronological age-matched peers and similar or weaker analogical reasoning competence compared to language-matched peers. To test these hypotheses, we use a scene analogy task composed of pictures of 20 relations (Richland et al., 2006) varying in relational complexity (binary or ternary relations) and in perceptual distraction. Twenty children with SLI are matched to chronological age and linguistic age peers without language disorders. Children with SLI have poorer performance then their age-matched peers but they have similar performance to their language-matched peers. This data reinforces the idea of a link between analogical reasoning and language, also in children with SLI. However, the nature of this link should still be clarified. [less ▲]

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See detailDisentangling soil from plant methanol exchanges in a maize field: a first step
Bachy, Aurélie ULg; Mozaffar, Ahsan ULg; Aubinet, Marc ULg et al

Poster (2016, June)

This poster presents the current research done in order to disentangle soil from plant methanol exchanges in a maize field. Methanol exchanges were calculated at ecosystem-scale (therefore including both ... [more ▼]

This poster presents the current research done in order to disentangle soil from plant methanol exchanges in a maize field. Methanol exchanges were calculated at ecosystem-scale (therefore including both components). It bases on the following observation: methanol exchanges on bare soil (measured when maize was at germination stage) were as important as when plants were fast growing (and thereby, when the highest methanol plant methanol emissions were expected), and this under similar weather conditions. The goal of this poster is thus to understand why emissions were similar at these two periods. First, it addresses the question of the actual contribution of maize plants in methanol exchanges, by comparing up-scaled methanol exchanges measured on maize at leaf-scale (Mozaffar A.) to those measured at ecosystem-scale. Then, it investigates methanol exchanges mechanisms in order to evaluate how did soil methanol emissions evolve along the maize growing season. At the end of this poster, the hypothesis of decreasing soil methanol emissions along the maize growing season is proposed. [less ▲]

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See detailMyoclonin-1 modulates the post-translational modification of microtubules
Medard, Laurie ULg; Coumans, Bernard ULg; Lakaye, Bernard ULg et al

Poster (2016, June)

Juvenile myoclonic epilepsy is one of the most common forms of generalized genetic epilepsy. Genetic studies have shown that heterozygous mutations in Myoclonin1 are responsible for 3-9% of clinical cases ... [more ▼]

Juvenile myoclonic epilepsy is one of the most common forms of generalized genetic epilepsy. Genetic studies have shown that heterozygous mutations in Myoclonin1 are responsible for 3-9% of clinical cases worldwide. This protein contains three DM10 domains of unknown function and an EF-hand domain. We have previously demonstrated that Myoclonin1 is a microtubule-associated protein involved in cell division and radial migration during neocortex development. In cells, this protein co-localized with specific structures rich in microtubules (MTs) such as the centrosome, the poles of the mitotic spindle or the motile cilia but not with cytoplasmic MTs. This suggests post-translational modifications (PTM) of MTs may be important for the interaction between Myoclonin1 and MTs. We have co-express the different enzymes catalyzing PTM of MTs with Myoclonin1 in U2OS cell line. With one of these enzymes, we observed a strong increase in PTM in the presence of Myoclonin-1. This suggests that Myoclonin1 may interact with and modulate the activity of this enzyme. By using luciferase complementation assay and pull down experiments, we could demonstrate that it is indeed the case. Interestingly, the effect is observed even when a DM10 domain alone is co-expressed with the enzyme, suggesting for the first time a role for this domain. In conclusion our data suggest myoclonin-1 modulates specific PTM of MTs. This is of prime importance for microtubule dynamic and notably for neuroblast precursor migration during neocortex development. This could be the mechanism that explains why pathological forms of myoclonin-1 affect brain development. [less ▲]

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See detailBeyond stuttering : Speech disfluencies in normally fluent, French-speaking children at age four
Leclercq, Anne-Lise ULg; Suaire, Pauline; Moyse, Astrid

Poster (2016, June)

The aim of the present study is to establish normative data concerning the speech disfluencies existing in normally fluent children speaking French at age 4, an age at which stuttering has begun in 95% of ... [more ▼]

The aim of the present study is to establish normative data concerning the speech disfluencies existing in normally fluent children speaking French at age 4, an age at which stuttering has begun in 95% of children who stutter (Yairi & Ambrose, 2013). [less ▲]

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See detailPhotoionization as a Soft Ionization Source for Comprehensive Two-dimensional GC (GCxGC) and High-Resolution TOFMS
Ubukata, M; Cody, RB; Dane, J et al

Poster (2016, June)

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See detailChronology of in vitro maturation of donkey (Equus asinus) oocytes collected by ovum pick up
Douet, Cécile; Goudet, Ghylène; Kaabouba-Escourier, Aurore et al

Poster (2016, June)

Most wild donkey breeds are currently endangered, such as the Asiatic and the African wild ass. Moreover, many domestic donkey breeds are vulnerable to extinction, such as the Martina Franca donkey in ... [more ▼]

Most wild donkey breeds are currently endangered, such as the Asiatic and the African wild ass. Moreover, many domestic donkey breeds are vulnerable to extinction, such as the Martina Franca donkey in Italy, the Zamorano-Leonés donkey in Spain and the Normand donkey in France. Their preservation includes the creation of a Genome Resource Bank. Embryos cryopreservation allows the preservation of genetics from both male and female and is the fastest method to restore a breed. However, embryo production in vivo is limited in equids. In vitro production of embryos would allow the production of several embryos per cycle, but no technique has yet been developed in the donkey for in vitro production of embryos. In that context, ovum pick up (OPU) would come as a very helpful tool both for embryo production and research purposes. Our objective was to develop OPU in jennies and to analyze the chronology of in vitro maturation of donkey oocytes. Donkey cumulus-oocyte complexes (COCs) were collected by OPU from 5 to 25mm follicles in adult cyclic jennies. During OPU, jennies were injected detomidine, butorphanol, and butylscopolamine for sedation, analgesia and antispasmodia. COCs were in vitro matured in TCM199 supplemented with fetal calf serum and epidermal growth factor for 24, 30, 34 or 38 hours in groups of 10 to 30 in an atmosphere of 5% CO2 in air at 38.5°C. Five OPU sessions were performed, with 3 to 6 females per session. We collected a total of 92 COCs out of 193 follicles (48%) with an average of 4.2 COCs per jenny. The recovery rate per follicle increased along the OPU sessions: 34%, 43%, 52%, 47% and 56%, probably due to the increasing skill of the staff. Despite a very large experience with OPU in horses, all staff members required some time to adjust to the specificities of the jenny vs mare, in particular to the differences in holding the ovary. All COCs were expanded after over 24 hours in vitro maturation (IVM). At collection, jennies COCs contained a germinal vesicle. After 24 hours IVM, COCs contained either a germinal vesicle or condensed chromatin. Metaphase 1 oocytes were observed after 30 hours IVM and 45% were in metaphase 2 after 34 hours IVM. Half of the COCs were degenerating after 38 hours IVM. In conclusion, we established for the first time conditions for ovum pick up in jennies and the chronology of in vitro maturation of donkey oocytes. We showed that in vitro maturation of donkey oocytes can produce 44% of metaphase 2 oocytes after 34 hours in culture while most equine oocytes reach metaphase 2 after 30 hours in culture. [less ▲]

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See detailImportance de l’utilisation du Système d’Information Géographique pour les vastes sites de plein air du Paléolithique moyen en France septentrionale. L’Exemple de Caours (Somme, France) et Beauvais (Oise, France).
Moreau, Gwénaëlle ULg; Locht, Jean-Luc

Poster (2016, June)

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement ... [more ▼]

During the middle Palaeolithic, the northern France was only occupied by Neanderthals groups. However, successions of glacial and interglacial cycles explain a complex and discontinuous settlement, raising complication in the study of its dynamics at a regional scale. Therefore, some aspects of Neanderthals behaviour are still mischaracterize: in most cases, the function of the site and its territorial management over the northern France have to be specified. The spatial analysis of open air sites from northern France will help us to answer those questions. However, for Middle Palaeolithic sites, we can’t see systematically on the field the spatial organisation directly, we sometimes need modelization. Therefore, we started to build a spatial analysis protocol adapted to this site and applied to such sites. The sites of Caours (Somme, France) and Beauvais (Oise, France) are two open air sites that are exceptionally well preserved and displaying a large amount of faunal and lithic rests. Both are perfect candidate to apply and test the new protocol. First results proved that for each site the existence of a spatial organisation as remains concentration zones. Then, we characterize this areas – number, distribution. Finally, we were able to associate them to human activity areas like hearths, butchery or knapping areas. [less ▲]

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See detailIntegrated microfluidic device for gold nanoparticles synthesis
Emonds-Alt, Gauthier ULg; Malherbe, Cédric ULg; Monbaliu, Jean-Christophe ULg et al

Poster (2016, May 31)

In order to perform the analysis of organic pollutant in trace by Raman spectroscopy, it is necessary to develop SERS substrates, such as noble metal nanoparticles (NPs). The synthesis of gold or silver ... [more ▼]

In order to perform the analysis of organic pollutant in trace by Raman spectroscopy, it is necessary to develop SERS substrates, such as noble metal nanoparticles (NPs). The synthesis of gold or silver NPs is used for analytical applications. It is therefore important to precisely control the synthesis of these SERS substrates to achieve reproducible measurements. Microfluidics is a very attractive technology for the control of the synthesis parameters such as temperature, reaction time and mixing conditions which affect both the size and the morphology of NPs. The synthesis of gold or silver NPs in microreactor is a technique we are already developing in the laboratory. We develop a method for the continuous synthesis of gold and silver NPs in a segmented regime capillary microreactor. The residence time and temperature have a significant effect on the size of the NPs, enabling to prepare different sizes of NPs using the same microreactor and the same precursor solutions. [less ▲]

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See detailGC×GC-HRTOFMS to better understand cloak-and-dagger activities of Madagascar Mantella frogs
Stefanuto, Pierre-Hugues ULg; Brasseur, Catherine; Cecchini, Harisoa et al

Poster (2016, May 30)

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See detailPeak dispersion evaluation and optimization in GC×GC
Stefanuto, Pierre-Hugues ULg; Dimandja, Jean-Marie; Focant, Jean-François ULg

Poster (2016, May 30)

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See detailGC×GC-TOFMS for forensic blood VOC profiling
Dubois, Lena ULg; Perrault, Katelynn ULg; Stefanuto, Pierre-Hugues ULg et al

Poster (2016, May 30)

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See detailEstetrol, a natural SERM exhibiting combined estrogenic and anti-estrogenic properties on mammary gland and breast cancer
Gallez, Anne ULg; Gérard, Céline ULg; Blacher, Silvia ULg et al

Poster (2016, May 30)

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would ... [more ▼]

The increased risk of breast cancer and thromboembolism in women who take hormone replacement therapy (HRT) is a major public health problem. The discovery of new drugs with better safety profile would provide useful advances for patient care. Estetrol (E4) is a liver friendly promising candidate for HRT. In preclinical and/or clinical studies, E4 has been effective against the main symptoms of menopause from a starting dose of 0.3 mg/kg/day. The aim of this study was to define the impact of E4 on mammary gland and breast cancer development. Our preclinical data show that E4 is less efficient than estradiol (E2) to induce mammary gland growth. Treatment with several concentrations of E4 has shown that E4 did not increase tumor development, when it is used at 0.3 mg/kg/day. However, at 3 mg/kg/day, E4 increased tumor growth similarly to E2 (0.08 mg/kg/day). E4 presents also some anti-estrogenic effects on mammary gland and antitumor activity on breast cancer by decreasing the strong proliferative effect of E2. While ERα is the predominant receptor mediating its effects, the dual weak-estrogenic/anti-estrogenic feature of E4 results from differential signaling pathways activation. Both nuclear and rapid extra-nuclear signaling pathways are necessary for a complete estrogenic effect of E4. However, the antitumor action of E4 is not due to a capacity to antagonize E2-induced nuclear activity. In conclusion, our results support that E4, if it is used in strictly controlled clinical applications, could have no or only limited impact on breast and breast cancer. [less ▲]

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See detailAtaxie optique: cas E.M.
DEMOULIN, Valentine ULg; Departz, Marie-Pierre; MAQUET, Pierre ULg

Poster (2016, May 28)

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See detailSubstrats cérébraux du contrôle proactif et réactif dans le vieillissement non pathologique
Manard, Marine ULg; François, Sarah ULg; Salmon, Eric ULg et al

Poster (2016, May 27)

Les recherches sur le contrôle cognitif proposent l’apparition d’un déclin lié à l’âge dans les capacités de contrôle proactif (forme anticipatoire de contrôle comportemental) alors que le contrôle ... [more ▼]

Les recherches sur le contrôle cognitif proposent l’apparition d’un déclin lié à l’âge dans les capacités de contrôle proactif (forme anticipatoire de contrôle comportemental) alors que le contrôle réactif (forme réactionnelle à la détection d’un conflit) semble rester intact [1]. Ces deux formes de contrôle ont été associées à des aires cérébrales différentes [2], cependant peu d’études ont étudié les effets de l’âge sur les régions cérébrales sous-tendant chacun de ces deux types de contrôle. Pour ce faire, une version adaptée de la tâche de Stroop a été administrée à 16 jeunes (18-30 ans) et 16 adultes (60-75 ans) en IRM fonctionnelle. Cette version de la tâche variait la quantité d’items interférents, permettant la création de trois contextes (Mostly Congruent « MC », Mostly Incongruent « MI » et Neutre) favorisant respectivement la mise en place des deux types de contrôle (réactif = congruent / proactif = incongruent). Les analyses ont été réalisées avec SPM8 (p<.001 non-corrigé). Les résultats comportementaux révèlent un traitement plus rapide de l’interférence dans le contexte « MI » que « MC » pour les jeunes adultes uniquement. Au niveau cérébral, le traitement de l’interférence en MI semble associé à une diminution d’activité (surtout à droite) dans les régions frontales et temporales chez les volontaires âgés par rapport aux plus jeunes, alors qu’en MC, les âgés montrent une augmentation d’activité bilatérale au niveau frontal. Ainsi, les résultats comportementaux montrent que les adultes âgés semblent avoir plus de difficultés à implémenter un contrôle proactif (associé à une diminution d’activité) que les jeunes dans les régions sous-tendant la performance à cette tâche. Cependant, le recrutement supplémentaire de régions frontales observé lors du contexte MC postulé pour favoriser le contrôle réactif, suggère la présence de mécanismes de compensation. Pour conclure, le vieillissement semble affecter de manière différentielle l’activité des régions cérébrales sous-tendant les différents types de contrôle. [less ▲]

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See detailSingle molecule force spectroscopy on helical foldamers
Devaux, Floriane ULg; Li, Xuesong; Huc, Ivan et al

Poster (2016, May 27)

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See detailGestalt Reasoning Promotes Extension Errors.
Dumitru, Magdalena ULg; Joergensen, Gitte

Poster (2016, May 27)

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See detailDESIGN OF HIGH-AFFINITY LIGANDS FOR THE BENZOTHIADIAZINE ALLOSTERIC BINDING SITE OF THE AMPA RECEPTORS
Drapier, Thomas ULg; Geubelle, Pierre ULg; Bouckaert, Charlotte et al

Poster (2016, May 26)

L-glutamic acid is the major excitatory neurotransmitter in the brain. It exerts its effects through metabotropic and ionotropic receptors. Among the latter, three subtypes have been identified: NMDA ... [more ▼]

L-glutamic acid is the major excitatory neurotransmitter in the brain. It exerts its effects through metabotropic and ionotropic receptors. Among the latter, three subtypes have been identified: NMDA, AMPA and KA receptors. It is now well established that a deficit in glutamatergic signaling may be responsible for neurological disorders such as mild cognitive impairment, schizophrenia, depression, and ADHD. Enhancement of the signal through positive allosteric modulators of AMPA receptors might be a therapeutic issue for these diseases. These compounds are expected to exert a fine tuning of the signal. Since they require the presence of the endogenous ligand to be active, they are expected to induce less toxicity than agonists. In this context, based on the structure of known allosteric modulators of AMPA receptors such as cyclothiazide and IDRA 21, the Laboratory of Medicinal Chemistry (University of Liège) has developed a series of 1,2,4-benzothiadiazine 1,1-dioxides with high potency as AMPA receptor potentiators. Crystallographic data obtained by the Department of Drug Design and Pharmacology (University of Copenhagen) highlighted those potentiators bind to two contiguous sites at the dimer interface of the ligand binding domain (LBD) of the AMPA receptor1,2. Based on these data, we may expect that the synthesis of dimeric molecules could lead to further improvement in affinity and activity. This assumption was reinforced by docking experiments conducted with virtual examples of dimeric compounds on the GluA2-LBD (collaboration with NAMEDIC). The present work is thus focusing on the preparation of a family of dimeric benzothiadiazine dioxides. Moreover, in collaboration with GIGA-Molecular Pharmacology, we are developing a pharmacological in vitro assay based on the measurement of Ca2+ inflow through a fluorimetric method. This medium-throughput screening will enable the characterization of our new compounds and the validation of our working hypothesis. [less ▲]

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See detailModelling of the β-lactamase BlaP induction in Bacillus licheniformis
Dauvin, Marjorie ULg; Raymackers, Alice ULg; Delvigne, Frank ULg et al

Poster (2016, May 24)

In bacteria, the production of a β-lactamase, an hydrolase specific to β-lactam antibiotics, may be constitutive or inducible. In Bacillus licheniformis 749/I the presence of a β-lactam in the external ... [more ▼]

In bacteria, the production of a β-lactamase, an hydrolase specific to β-lactam antibiotics, may be constitutive or inducible. In Bacillus licheniformis 749/I the presence of a β-lactam in the external media is detected by a protein relay producing an intracellular signal which leads to the induction of BlaP β-lactamase expression. The blaP gene is included in a divergeon along with blaI, coding for a cytoplasmic repressor, and blaR1, coding for a penicillin membrane receptor. Both, the acylation of the extracellular domain of BlaR1 by a β-lactam together with cellular stress due to the presence of the antibiotic outside the cell generate a dipeptide (coactivator) resulting from the peptidoglycan turnover that destabilizes BlaI repressor-DNA complex, leading to the expression of β-lactam resistance. [less ▲]

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See detailIntimate partner violence: Perseverance vs Dropout from treatment programs targeting male batterers
Di Piazza, Laetitia ULg; Kowal, Cécile; Hodiaumont, Fabienne et al

Poster (2016, May 24)

Introduction: The Dropout phenomenon has been studied extensively in general psychotherapy and in more specific domains. Therapeutic programs for male batterers across Europe, United States or Canada also ... [more ▼]

Introduction: The Dropout phenomenon has been studied extensively in general psychotherapy and in more specific domains. Therapeutic programs for male batterers across Europe, United States or Canada also have their share of problems in their attempts to maintain users in treatment. The purpose of this study is to identify potential intrapsychic variables associated with dropout and completion of this kind of treatment. Method: Fifty one male offenders enrolled in a group treatment for domestic violence took part in the study (23 who abandoned and 28 who completed). Before the start of therapy, they were surveyed using questionnaires and structured clinical interview to collect sociodemographic data and to assess specific psychological variables, namely emotional distress (BDI), impulsive behavior (BIS-11), early relationship with their parents (PBI), life events (LEDS) and alexithymia (TAS-20), the inability to experience and express subjective emotions. Results: Correlational analysis showed that age, paternal parenting behaviors and the number of significant events reported in the past six months are the only variables correlated with treatment dropout. The younger the participants, the most likely they were to dropout of the group therapy. Moreover, men who completed treatment reported fewer difficulties in their life and less autonomy support by their father than men who prematurely terminated their therapy. Discussion: The present findings pose a serious challenge for all actors involved in specifics treatment programs. Indeed, if all individuals who committed violent acts have the same intrapsychic variables, how do we predict, especially retain and motivate, the users likely to dropout? [less ▲]

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See detailA phenomenological approach of anorexia nervosa
Minguet, Eugénie; Englebert, Jérôme ULg; Helinski, Adam et al

Poster (2016, May 24)

Introduction: from a phenomenological perspective, anorexic female patients seem to experience difficulties regarding the subjective feeling of their own body (embodiment) and their personal identity ... [more ▼]

Introduction: from a phenomenological perspective, anorexic female patients seem to experience difficulties regarding the subjective feeling of their own body (embodiment) and their personal identity, which lead to an intersubjectivity impairment within social interactions. Following these findings, this study investigates the “body-for-others” concept (Sartre, 1943) and altruistic hypotheses from the ethologist Albert Demaret (1973) in a sample of anorexic female patients. Two main hypotheses are tested: anorexic patients are expected to define themselves mostly through the gaze of others and as more altruistic than control participants. Method: this study included 67 anorexic female patients (based on the DSM-IV criteria) and 246 control female participants. Three self-reported questionnaires were used: the Eating Disorder Inventory: EDI-II of Garner (French version), the Identity and Eating Disorders questionnaire: IDEA of Stanghellini (French version translated and adapted by us) to assess the “body-for-others” concept and 17 complementary items for the altruistic dimension. Mann-Whitney non-parametric tests were used to compare the two groups. Results: results show that anorexic patients obtain higher scores than the control group for the three questionnaires (p<0,001 for all measurements). Discussion: the IDEA questionnaire enriches the knowledge relative to the body-self relationship and the first-person perspective, which is essential for a phenomenological approach. Furthermore, the promising results concerning the altruistic dimension in anorexia nervosa should be evaluated in future research. Nevertheless, a better validity of the items assessing the altruistic dimension is needed. [less ▲]

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See detailAbnormal bodily experiences in schizophrenia: a phenomenological study
lancellotti, Elisa; Englebert, Jérôme ULg

Poster (2016, May 24)

Our study focuses on Abnormal Bodily Experiences [ABE] in people with schizophrenia. We have created a specific questionnaire for this study – based on the study of Stanghellini et al. (2012) and the ... [more ▼]

Our study focuses on Abnormal Bodily Experiences [ABE] in people with schizophrenia. We have created a specific questionnaire for this study – based on the study of Stanghellini et al. (2012) and the third dimension of the EASE scale (body experiences) (Parnas et al. 2005). Our research follows an analytical method called “empirical-phenomenological”. Our population is made up of 50 schizophrenic subjects. The average age of subjects was 37 years. There are no exclusion criteria regarding gender or control sample. Analyzes are qualitative. We observed that ABE are very present symptoms in people with schizophrenia. Indeed, in our sample, only one patient was asymptomatic. We also observed that all categories and subcategories were present in our sample. These results should help to refine and clarify the diagnosis of schizophrenia and open up significant opportunities for psychotherapeutic dimension. [less ▲]

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See detailHéboïdophrenia: The hazardous interplay of psychosis and psychopathy
De Page, Louis; Englebert, Jérôme ULg

Poster (2016, May 24)

Introduction The diagnostic entity of heboidophrenia has been consigned to oblivion despite its clinical, prognostic, criminogenic value. At the core of this diagnosis lies specifics interactions between ... [more ▼]

Introduction The diagnostic entity of heboidophrenia has been consigned to oblivion despite its clinical, prognostic, criminogenic value. At the core of this diagnosis lies specifics interactions between psychosis and psychopathy. The diagnosis was first coined by Kalhbaum in 1890, was revived by Anglo-Saxon literature in the first half of the 20th century, and by French psychiatrists in the late 90’. Method We integrated literary sources, clinical experience and an own pilot study as a stepping-stone for a more in-depth research. Cases (n=20) were selected out of samples of Mentally Disordered Offenders in Belgian Medium and High Risk Forensic Facilities. Files, records and therapist’s experiences were quantitatively analysed iteratively to cull subtypes from each other. We here present preliminary qualitative results, more indepth qualitative and quantitative results will be produced. Results The diagnosis subsumes more than a mere DSM-IV-TR Axis I & II comorbidity, it can be seen from a developmental perspective, or from a structural perspective. Both vantage points suggest an interplay between the (paranoid) psychotic process and the antisocial behaviour, where both trigger and induce each other. At this point, we found evidence for three subtypes modes of interplay; 1) mood instability patterns induced either psychopathic or psychotic phenotypic presentation, 2) according to personality organization and its defensive functioning (were psychopathy defends against psychosis), and 3) phenotypic expression of both components (psychopathy and psychosis) linearly co-dependent. Discussion Despite its incremental value, little is known about the therapeutic prospects of this diagnosis. Current results indicate that these patients do question competency to answer for their deeds in a peculiar manner, relapse more often, reoffend more often, do evade treatment opportunities (if they have any), act out more often (in both antisocial and psychotic ways), induce a particular negative counter-transference in psychiatric teams, etc. Because of these particularities and their versatile phenotype presentation, heboidophrenia patients cannot simply be assimilated to the “high risk & high care” population, but need special supervision and clinical insight. In this research, we aim(ed) at discerning semiologic signs in order to support the diagnostic process. [less ▲]

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See detailPeptidoglycan fragments separation by CE/LC-MS
Boulanger, Madeleine ULg; Delvaux, Cédric ULg; Far, Johann ULg et al

Poster (2016, May 24)

In Bacillus licheniformis 749/I, the induction of BlaP beta-lactamase relies on a complex regulation system. During this process, the intracellular repressor BlaI is inactivated by a dipeptide coming from ... [more ▼]

In Bacillus licheniformis 749/I, the induction of BlaP beta-lactamase relies on a complex regulation system. During this process, the intracellular repressor BlaI is inactivated by a dipeptide coming from the peptidoglycan (PG) degradation via an “AND Gate” regulation. This regulation involves the cellular stress induced by the beta-lactam, the membrane receptor BlaR1 and the PG turnover. Briefly, the induction occurs when the extracellular domain of BlaR1 is acylated by the antibiotic which leads to a reorganization of the transmembrane segments and the receptor autocleavage. Simultaneously, the beta-lactam partially inhibits the penicillin-binding protein 1 (PBP1), triggering increased PG turnover and accumulation of PG fragments. Some of these fragments could enter in the cytoplasm and undergo enzymatic degradations which lead to the formation of the pro co-activator (tripeptide L-Ala-D-Glu-m-A2pm). This pro co-activator generates the co-activator, the dipeptide D-Glu-m-A2pm. Nowadays the nature and the concentration of PG fragments inside the cytoplasm are unknown. Therefore, the development of different analytical methods is required in order to identify those cytoplasmic fragments. In this poster, three different ways to separate PG fragments are discussed. [less ▲]

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See detailMaternal sense of competence in a context of precariousness
Halin, Stéphanie ULg; Glowacz, Fabienne ULg

Poster (2016, May 24)

The exercise of parenthood is threatened by the precarious context in which parents and children live. Precariousness is defined by the lack or the absence of basic securities, such as housing, employment ... [more ▼]

The exercise of parenthood is threatened by the precarious context in which parents and children live. Precariousness is defined by the lack or the absence of basic securities, such as housing, employment, financial, emotional security, etc. In such a context, the emotional availability of a parent can be reduced, so that the quality of parent-child interactions decreases and multiple dimensions of the child development are impacted. Therefore, policies of prevention and intervention have been developed in order to overcome the difficulties experienced by parents from birth and, sometimes, from pregnancy. However, little is known about the parenting sense of competence of mother living in such a precarious context, and the possible mediating effect of perceived social support. In this study, we investigate parenting sense of competence in a sample of 38 adult mothers raising alone their children in a context of poverty. Subjects completed the French versions of the “Parenting Sense of Competence Scale (P.S.O.C.)” and the “Social Provisions Scale”, and a socio-demographic questionnaire. Results indicate that, in average, mothers report a high parenting sense of competence (μ=69.18), characterized by a high level of self-efficacy (μ=32.76) and an average level of satisfaction (μ=36.42). Mothers also report low perceived social support (T=41, percentile 21). Results also indicate a significant negative correlate between self-efficacy and the need to feel useful and necessary, while the satisfaction dimension is positively correlated with all the perceived social support dimensions, except from the “need to feel useful” dimension. In conclusion, mothers raising alone their children in a context of poverty report high levels of parenting sense of competence. However, withdrawal and isolation are associated with poorer parental satisfaction. This suggests that the social dimension of poverty is a significant aspect to consider in parenting support policies. [less ▲]

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See detailWould you care for some integrated care in your fragmented health system? A participatory action research to improve integration between levels of care in a Belgian urban setting.
Belche, Jean ULg; Duchesnes, Christiane ULg; Darras, Christian et al

Poster (2016, May 24)

Integration between levels of care is not facilitated by the Belgian health system. Indeed, patients have uninhibited access to every level of care, there is no gatekeeping system, and no structural ... [more ▼]

Integration between levels of care is not facilitated by the Belgian health system. Indeed, patients have uninhibited access to every level of care, there is no gatekeeping system, and no structural coordination between levels of care. Meanwhile, on one hand, the occurrence of more complex care situations in the ambulatory setting is enhancing the need for coordination while on the other hand, hospitals face financial constraints to provide care in the community. The aim of the research was to organize coordination between levels of care at the local level, in an urban setting. We used the “Local Health System” model (LHS) that aims at integrating hospital and primary care activities for a defined population at the local level. We chose the participatory action research (PAR) methodology and its spiral plan-do-study-act cycles, to ensure the participation and implementation of results by the targeted actors (hospital and local general practitioners (GP) organizations). [less ▲]

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See detailIncarceration and parenting : is parenting a desistance factor?
Puglia, Rosa ULg; Servaes, Anne; Halin, Stéphanie ULg et al

Poster (2016, May 24)

Incarceration leads to a break between an individual and one’s social environment, inducing significant losses of identity (Touraut, 2014). Tough delinquent identity remains prominent, during their ... [more ▼]

Incarceration leads to a break between an individual and one’s social environment, inducing significant losses of identity (Touraut, 2014). Tough delinquent identity remains prominent, during their incarceration, some inmates invest their role of father. Investing his role of father and thus his father’s identity allows the inmate to break away with delinquent’s identity (Tripp, 2009; Walker, 2009). Moreover, research on desistance underlines that turning point like job, marriage or parenthood supports the transition from criminal life to conventional life (Laub et Sampson, 2003; Lebel, Burnett, Maruna & Bushway, 2008). Indeed, becoming parent is a stage in life cycle that can shape one’s lifestyle. From qualitative researches conducted with father inmates since at least 6 months, we have identified the link between investment in parenting and desistance. The hypothesis is that investment in parenting has a positive impact on father’s prison experience, parenting sense of competence and it will support desistance process. A semi-structured interview and several questionnaires assessing relational and social life have been used in this context (WHOQOLbrief, EPS, ESUL, educational attitudes, QAECEP). First results highlight that parenting during incarceration can be difficult. Some inmates try to keep it with visits between inmate fathers and their children. These visits are influenced by different factors and have a positive impact on prison experience. Indeed, with these visits, inmates maintain a link with their children. That is a source of support and hope during incarceration. That allows investment in one father’s identity. [less ▲]

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See detailLife cycle assessment of hemp concret blocks
Groslambert, Sylvie ULg; de Mahieu, Jean-Baptiste; Léonard, Angélique ULg

Poster (2016, May 24)

Buildings notably contribute to global environmental negative impacts due to consumption of both embodied energy and natural resources as well as various emissions during their whole life cycle. It is ... [more ▼]

Buildings notably contribute to global environmental negative impacts due to consumption of both embodied energy and natural resources as well as various emissions during their whole life cycle. It is therefore necessary to develop practices to reduce these impacts, mainly by reducing the part of non-renewable resource in material as well as by ensuring the lowest energy consumption possible during their lifetime. New developments in natural fibres and their use in insulating materials can lead to significant improvement in building environmental impact. For this purpose, assessment of environmental performance is needed to support both the design and the production of (new) fibre based insulation solutions. In this context, the Life Cycle in Practice (LCiP) project helps SMEs to reduce the environmental impacts of their products and services across the entire life cycle. Within the frame of this project, Isohemp (BE) hemp concrete block impact is evaluated in a cradle-to-gate LCA. Functional unit is a pallet of hemp concrete blocks ready for shipping. It represents about 1.3 m³ of blocks. Hemp blocks are made by pressing a mix of hemp shives, hydraulic and hydrated limes, and water. Long term carbon storage due to lime carbonation induces a large benefit in Climate Change (CC) category. The balance of the CC indicator for hemp cultivation is also favorable due to carbon dioxide uptake by the photosynthesis occurring during the plant growth. Life Cycle Assessment of hemp concrete blocks ready to ship has also highlights some improvements that can easily be made at the packaging level in order to lower the global environmental impact and increase the sustainability of this insulation material. Data are processed in SimaPro 8 software, with Ecoinvent 3 and ELCD 3 databases, and analyzed with the CML IA method. This method is compliant with the indicators required by EN15804 standard in order to communicate on the environmental performance of Isohemp blocks. [less ▲]

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See detailFe2O3 nanoparticle-functionalized N-doped carbon with interconnected, hierarchical porous structures as high-performance electrode for lithium ion batteries
Alkarmo, Walid ULg; Ouhib, Farid ULg; Aqil, Abdelhafid ULg et al

Poster (2016, May 23)

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous ... [more ▼]

Thanks to their fascinating physical properties such as high surface area, multidimensional electron transport pathways and good mechanical strength, three dimensionally (3D) interconnected carbon porous frameworks have emerged as attractive materials for various electrochemical energy storage/conversion devices, including Li-ion batteries (LIBs), Li−O2 batteries, Li−S batteries, supercapacitors, and fuel cells. A hierarchically structured macro- and mesoporous N-doped carbon with dispersed Fe2O3 nanoparticles (NDC@Fe2O3) is prepared by thermal treatment of a novel composite composed by PMMA particles decorated by graphene oxide (GO), PPy and iron salts. The NDC@Fe2O3 composite exhibited high surface area with a hierarchical pores structure. Integrated as a lithium ion battery anode, NDC@Fe2O3 exhibited high reversible capacity of 930 mA h/g over 200 cycles. The combination of Fe2O3 nanoparticles with porous carbon to form hybrid anode has been an efficient way to maintain the electronic integrity of the whole electrode since the carbon acts as a buffer layer to accommodate the volume variation and to provide multidimensional electron transport pathways during the charge/discharge process. [less ▲]

Detailed reference viewed: 360 (9 ULg)
See detailSynthesis of novel well-defined poly(vinylamine)-based copolymers by controlled polymerization
Dréan, Mathilde; Guégan, Philippe; Detrembleur, Christophe ULg et al

Poster (2016, May 23)

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful ... [more ▼]

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful in many fields such as coatings , water purification and gas membrane separation. The properties of such polymers strongly depend on the nature of the amines and can thus be adjusted by incorporating different types of amines along the backbone. In this perspective, we developed a strategy for synthesizing poly(vinylamine)-based (co)polymers containing primary and secondary amines as well as imidazole moieties in predictable proportions. First, radical (co)polymerizations of N-vinylacetamide, N-methylvinylacetamide and vinylimidazole were performed followed by deprotection of the amine functions via acidic hydrolysis of the pendant amides. We determined the reactivity ratios of each comonomer pairs and developed the corresponding Skeist’s model, allowing the prediction of the copolymer compositions and distributions. Following this straightforward approach, novel amine-containing copolymers with predictable and precise compositions were made available and should contribute in the future to the development of the above-mentioned applications. [less ▲]

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See detailTuning morphology and surface properties of emulsion-templated porous polymers by controlled radical polymerization
Mathieu, Kevin ULg; Jérôme, Christine ULg; Debuigne, Antoine ULg

Poster (2016, May 23)

Macroporous polymer monoliths with interconnected structures have attracted considerable interests in the last decades and sustain many applications in the fields of supported catalysis, chromatography ... [more ▼]

Macroporous polymer monoliths with interconnected structures have attracted considerable interests in the last decades and sustain many applications in the fields of supported catalysis, chromatography, microfluidic, water purification, membrane separation, tissue engineering, to name but a few. A very popular and straightforward synthetic approach for such open-cell polymers is the High Internal Phase Emulsion (HIPE) polymerization method. The latter consists in three steps: (i) stabilization of an emulsion in which an internal phase with a volume fraction higher than 74% is dispersed within a minor continuous phase containing a polymer network precursor, (ii) curing of the polymer phase by polymerization or crosslinking reactions, (iii) removal of the internal phase to release the porosity. The surface properties and morphology of the polyHIPEs are important features that must be controlled and adjusted for each applications. In this context, we prepared by controlled radical polymerization a series of well-defined amphiphilic copolymers having different hydrophilic-lipophilic balance (HLB) and chain-ends, used it as stabilizers for the emulsion-templated polymerizations and demonstrated the crucial effect of the structure of the macromolecular surfactant on the morphology of the polyHIPEs. Finally, the physical and chemical anchoring of the macromolecular surfactants at the surface pores were also considered for tuning the surface properties of the porous monoliths. [less ▲]

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See detailSynthesis of novel poly(vinylamine)-based copolymers with controlled compositions
Dréan; Guégan, Philippe; Detrembleur, Christophe ULg et al

Poster (2016, May 23)

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful ... [more ▼]

Metal binding, pH sensitivity and polyelectrolytes complexation are key characteristics of amine-containing polymers. Among them, poly(vinylamine)s presenting high amine densities are particularly useful in many fields such as coatings , water purification and gas membrane separation. The properties of such polymers strongly depend on the nature of the amines and can thus be adjusted by incorporating different types of amines along the backbone. In this perspective, we developed a strategy for synthesizing poly(vinylamine)-based (co)polymers containing primary and secondary amines as well as imidazole moieties in predictable proportions. First, radical (co)polymerizations of N-vinylacetamide, N-methylvinylacetamide and vinylimidazole were performed followed by deprotection of the amine functions via acidic hydrolysis of the pendant amides. We determined the reactivity ratios of each comonomer pairs and developed the corresponding Skeist’s model, allowing the prediction of the copolymer compositions and distributions. Following this straightforward approach, novel amine-containing copolymers with predictable and precise compositions were made available and should contribute in the future to the development of the above-mentioned applications. [less ▲]

Detailed reference viewed: 43 (3 ULg)