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See detailRelative Contribution of Walking Speed, Ataxia and Gait asymmetry to the Composition of Gait in Multiple Sclerosis
PHAN BA, Remy ULg; Pierard, Sébastien ULg; LOMMERS, Emilie ULg et al

Poster (2013, October)

Introduction - Objective: Walking speed measured according to the T25FW is the most widely used descriptor of gait in MS clinical research and practice but other dimensions influencing gait variance exist ... [more ▼]

Introduction - Objective: Walking speed measured according to the T25FW is the most widely used descriptor of gait in MS clinical research and practice but other dimensions influencing gait variance exist according to alternative gait analysis methods. The relative importance of these different dimensions of gait relatively to its variance is unknown. Methods: We measured the performances of persons with MS and healthy subjects on the T25FW and the Timed 20-Meter Walk (T20MW) performed in tandem with a new gait analysis system (GAIMS). We performed a factorial analysis of variance to underline the main dimensions influencing gait variance and observed their composition. Findings - Conclusion: The main factor influencing gait variance in conventional walk tests is mostly composed of features related to walking speed. Balance, gait asymmetry and variability also participate to this variance but to a lesser extent. The inverse is observed in tests performed in tandem gait. [less ▲]

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See detailComet dust profiles from PACS images obtained in the framework of the HSSO project
Opitom, Cyrielle ULg; Bockelée-Morvan, D.; Hutsemekers, Damien ULg et al

Poster (2013, October)

In the framework of the HssO project the Herschel PACS instrument acquired images of 7 comets between June 2010 and February 2013. Three of these comets have been imaged at several heliocentric distances ... [more ▼]

In the framework of the HssO project the Herschel PACS instrument acquired images of 7 comets between June 2010 and February 2013. Three of these comets have been imaged at several heliocentric distances allowing us to follow up the evolution of the dust coma . Radial profiles have been derived for each image. We measured flux densities at 70, 110 and 160 μm in order to determine the comet dust production rate. In some cases, after deconvolution by the instrumental PSF, we might have detected the nucleus signal in the central pixels. [less ▲]

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See detailYellow Hypergiant /Luminous Blue Variable
Cox, N. L. J.; Hutsemekers, Damien ULg; Vamvatira-Nakou, Chloi ULg

Poster (2013, October)

Hen3-1379 is a yellow hypergiant (YHG) with evidence for episodic non-quiescent mass-loss over in last 400-1000 years in this post-red supergiant (RSG) phase of its evolution. The observational evidence ... [more ▼]

Hen3-1379 is a yellow hypergiant (YHG) with evidence for episodic non-quiescent mass-loss over in last 400-1000 years in this post-red supergiant (RSG) phase of its evolution. The observational evidence suggests that though similar to the low-luminosity LBV Wray15-751, it has not yet moved to the hotter phase and is thus pre-LBV. This confirms the scenario of significant mass-loss during the RSG phase. [less ▲]

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See detailMolecular mechanisms of type I collagen-induced apoptosis in breast carcinoma cells
Maquoi, Erik ULg; Assent, Delphine ULg; Foidart, Jean-Michel ULg et al

Poster (2013, September 27)

Objective: As invading breast carcinoma cells breach the underlying basement membrane, they become confronted with a dense three-dimensional reactive stroma dominated by type I collagen. To develop ... [more ▼]

Objective: As invading breast carcinoma cells breach the underlying basement membrane, they become confronted with a dense three-dimensional reactive stroma dominated by type I collagen. To develop metastatic capabilities, invading tumour cells must acquire the capacity to negotiate this hostile microenvironment. By enmeshing cells in a dense fibrillar network, type I collagen acts as a physical barrier for cell migration as well as an endogenous antigrowth signal, partly by inducing apoptosis in epithelial cells. Aberrant cell survival resulting from an acquired resistance toward apoptosis represents a prominent hallmark of cancers. However, the molecular mechanisms implicated in collagen-induced apoptosis remain poorly defined. Here, we investigate the molecular mechanisms by which type I collagen induces apoptosis in breast carcinoma cells and identify MMP-14, a membrane-anchored matrix metalloproteinase, as a key anti-apoptotic factor. Methods: To investigate the induction of apoptosis by collagen, human breast adenocarcinoma MCF-7 cells overexpressing or not MMP-14 were plated on plastic plates or embedded within three dimensional type I collagen gels (Col3D). Cell death was evaluated by measuring cytoplasmic histone-associated DNA fragments (Cell Death Detection ELISA). The percentage of cells with an apoptotic nuclear morphology was also determined. The interactions between cancer cells and Col3D were analyzed by confocal microscopy and the impact of Col3D on the transcriptome of cancer cells was investigated using Illumina HT-12 BeadArrays. Results: When cultured within Col3D gels, MCF-7 cells displayed a round morphology and a cell death characterized by a Z-VAD-FMK-dependent chromatin condensation, nuclear segmentation and oligonucleosomal DNA fragmentation was induced. Transfection of MCF-7 cells with MMP-14 cDNA promoted the interactions between cells and collagen and prevented apoptosis. A transcriptomic analysis revealed that culturing MCF-7 cells within Col3D altered the expression of about 700 genes, irrespective of MMP-14 expression. Col3D modulated the expression of several apoptosis-related genes. Interestingly, MMP-14 activity was sufficient to prevent the Col3D-dependent induction of Bcl2-Interacting Killer (BIK), a pro-apoptotic member of the Bcl-2 family. Conclusions: Our results shed light on the molecular mechanisms by which a collagen-rich microenvironment triggers apoptosis in invading breast cancer cells. Furthermore, we demonstrate that MMP-14 promotes tumour progression by circumventing apoptosis. [less ▲]

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See detailPILOT PROJECT IN CHR-LIEGE: INVITING SIBLINGS OF CHILDREN WITH CANCER TO A CREATIVE AND EXPRESSIVE WEEK-END WITH PSYCHOLOGISTS AND ONCOLOGIST
Forget, Patricia; Badot, Isabelle; Missotten, Pierre ULg et al

Poster (2013, September 27)

The siblings week end with the psycho-medical team was highly successful It opens another way to improve the care of the siblings Questionnary forms should be developped in the future to evaluate the real ... [more ▼]

The siblings week end with the psycho-medical team was highly successful It opens another way to improve the care of the siblings Questionnary forms should be developped in the future to evaluate the real psychological impact [less ▲]

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See detailResorcinol-Formaldehyde Carbon Xerogels as Anode Material for Lithium-Ion Battery: Synthesis, Grinding and Coating on Current Collector
Piedboeuf, Marie-Laure ULg; Léonard, Alexandre ULg; Pirard, Jean-Paul ULg et al

Poster (2013, September 24)

Rechargeable lithium-ion batteries show great advantages over traditional batteries and are extensively used for consumer electronic devices due to their high energy density and long cycle life. However ... [more ▼]

Rechargeable lithium-ion batteries show great advantages over traditional batteries and are extensively used for consumer electronic devices due to their high energy density and long cycle life. However, the improvement of performance of current lithium-ion batteries requires the optimization of the materials used (electrolyte and electrodes). Therefore, tremendous efforts have been dedicated to exploring new materials with high capacity, excellent cycling performance, low cost and high safety features [1-3]. As an example, carbon xerogels are promising candidates in the development of new high performance C-based anode materials for Li-ion batteries, since such carbonaceous materials show very small changes of volume during the charge/discharge process, providing a long cycle life. Nevertheless, hard carbons also exhibit quite high irreversible capacity losses due to their intrinsic high microporosity [4]. To overcome these disadvantages, the structural and textural characteristics need to be carefully controlled. Also, due to the different morphology of these materials compared to graphite, the deposition of carbon xerogels on current collectors needs to be studied in detail. In this work, porous carbon xerogels have been synthesized from Resorcinol-Formaldehyde mixtures by adjusting the pH of the solution in order to obtain different mesopore sizes. Monoliths of carbon xerogels are obtained after drying of the polymer gel and pyrolysis [5]. These monoliths have been ground by two different methods and particle size distributions were measured by granulometry. Mercury intrusion porosimetry and nitrogen adsorption techniques (BET) have been used to characterize the pore texture of the monolithic and the powder materials. Different conditions have been used for the mixing of carbon xerogels with a binder and a solvent to form slurries. The latter have been cast on a copper foil using bar coating with different openings. After evaporation of the solvent, the resulting coatings were analyzed using scanning electron microscopy (SEM) for the morphology and their thickness was monitored by profilometry. First results indicate that the method of grinding has no influence on the final particle size distribution of the powder. The structural features of the carbon xerogels is well preserved for particles down to one micrometer. Nevertheless, a study of grinding duration shows that additional particles with sizes close to that of the porosity of the carbon appear. As a consequence, the grinding conditions were chosen so as to obtain a compromise between particles small enough to realize a coating on a current collector and particles large enough to maintain the carbon gel structural characteristics. References 1) Goodenough J.B., Kim Y. J. Power Sources 2011; 196(16): 6688-6694. 2) Bruce P.G. Solid State Ionics 2008; 179: 752-760. 3) Cairns A. J., Albertus P. Ann. Rev. Chem. Biomol. Eng. 2010; 1: 299-320. 4) Tran T., Yebka B., Song X., Nazri G., Kinoshita K., Curtis D. J. Power Sources 2000; 85: 269-278. 5) Job N., Théry A., Pirard R., Marien J., Kocon L., Rouzaud J., Béguin F., Pirard J. Carbon 2005; 43: 2481-2494. [less ▲]

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See detailApplication of the Data-Interpolating Variational Analysis (DIVA) to sea-level anomaly measurements in the Mediterranean Sea
Troupin, Charles ULg; Barth, Alexander ULg; Beckers, Jean-Marie ULg et al

Poster (2013, September 23)

In ocean sciences, numerous techniques are available for the spatial interpolation of in situ data. These techniques mainly differ in the mathematical formulation and the numerical efficiency. Among them ... [more ▼]

In ocean sciences, numerous techniques are available for the spatial interpolation of in situ data. These techniques mainly differ in the mathematical formulation and the numerical efficiency. Among them, DIVA, which is based on the minimization of a cost function using a finite-element technique (figure 1). The cost function penalizes the departure from observations, the smoothness or regularity of the gridded field and can also include physical constraints. The technique is particularly adapted for the creation of climatologies, which required a large to several regional seas or part of the ocean to generate hydrographic climatologies. Sea-level anomalies (SLA) can be deduced from satellite-borne altimeters. The measurements are characterized by a high spatial resolution along the satellite tracks, but often a large distance between neighbour tracks. This implies the use of simultaneous altimetry missions for the construction of gridded maps. An along-track long wave-length error (correlated noise, e.g. due to orbit, residual tidal correction or inverse barometer errors) also affects the measurement and has to be taken into account in the interpolation. In this work we present the application and adaptation of Diva to the analysis of SLA in the Mediterranean Sea and the production of weekly maps of SLA in this region. Determination of the parameters The two main parameters that determines an analysis with DIVA are the correlation length (L) and the signal-to-noise ratio (SNR). Because of the particular spatial distribution of the measurements, the tools implemented in Diva for the analysis parameter determination tend to underestimate L and overestimate SNR, leading to noisy analysis (the observation constraint dominates the regularity constraint). Some adaptations of the tools are necessary to solve this issue. Numerical cost Because of the large number of observations to be processed (in comparison with in situ measurements on a similar period), the interpolation method employed is expected to be numerically efficient. Improvements in the implementation of Diva further improved the numerical performance of the method, especially thanks to the use of a parallel solver for the matrix inversion. The performance of finite-element mesh generator was also enhanced, so that interpolation of a data set of more than 1 million data points on a 100-by-100 grid can be performed in a few minutes on a personal laptop. Analysis and error field The analysis and error fields obtained over the Mediterranean Sea are compared with the available gridded products from AVISO. Different ways to compute the error field are compared. The impact of the use of multiple missions to prepare the gridded fields is also examined. [less ▲]

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See detailAnalytical study of “La Famille Soler” by Picasso: from the Blue Period to Cubism
Defeyt, Catherine ULg; Vekemans, Bart; Vandenabeele, Peter et al

Poster (2013, September 23)

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See detailOrthographic learning in adult skilled readers
Binamé, Florence ULg; Poncelet, Martine ULg

Poster (2013, September 21)

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See detailPerception du pouvoir entre visiteurs et visités dans la pratique du tourisme dit « ethnique »: Au-delà des oppositions, authentique / simulacre
Lekane Tsobgou, Dieudonné ULg; Schmitz, Serge ULg

Poster (2013, September 20)

La pratique du tourisme est prise sous l'angle d'une confrontation entre les peuples « visiteurs » et les peuples « visités » (Demanget, 2007). L’exotisme culturel des populations autochtones est la ... [more ▼]

La pratique du tourisme est prise sous l'angle d'une confrontation entre les peuples « visiteurs » et les peuples « visités » (Demanget, 2007). L’exotisme culturel des populations autochtones est la principale attraction (Harron et al. 1992). Ainsi, une modification de l’équilibre et une rencontre bilatérale semblent plus opaques. Dès lors, les produits authentiques ou les productions instrumentalisées déterminent la position de pouvoir détenus par les visiteurs ou par les visités. Les promoteurs touristiques cherchent avant tout leur gain d’où l’offre parfois des produits issus de simulacre aux visiteurs en mal d’exotisme. Comment illustrer les jeux de pouvoir et de marchandage qui organisent les rencontres plus ou moins authentiques entre visités et touristes ? Les pratiques du tourisme ethnique en Afrique (Cameroun, Madagascar) et en Asie ( Birmanie, Thailande) nous ont servi d'outils d'analyse et d'expérimentation du jeu de pouvoirs entre visités et visiteurs. [less ▲]

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See detailStudy of the estrogenicity of pure compound migrate from plastic food contact materials
Simon, Coraline ULg; Oghena, M.; Covaci, A. et al

Poster (2013, September 19)

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See detailDrug delivery systems based on amphiphilic polyphosphate-copolymers
Vanslambrouck, Stéphanie ULg; Clement, Benoît ULg; Riva, Raphaël ULg et al

Poster (2013, September 18)

Thanks to their biocompatibility, biodegradability and their structure similar to natural biomacromoleculesn such as nucleic acids, polyphosphates (PPhos) are of prime interest as biomaterials. In ... [more ▼]

Thanks to their biocompatibility, biodegradability and their structure similar to natural biomacromoleculesn such as nucleic acids, polyphosphates (PPhos) are of prime interest as biomaterials. In contrast to poly--caprolactone and polylactides, PPhos properties and functionality are easily tuned via the nature of the pendant group of the starting cyclic monomer. For example, by varying the length of the alkyl chain the hydrophobicity of the PPhos can be adjusted. In this work, an efficient organo-catalytic system was developed to synthesize a series of amphiphilic diblock copolymers, i.e. poly(ethylene oxide)-b-polyphosphate (PEO-b-PPhos) by ring-opening polymerization of cyclic phosphates. This novel approach prevents metallic residues to polute the final product, and which is highly desirable when biomedical applications are foreseen. For drug delivery application, the micellization of these novel diblock copolymers in aqueous media was investigated, as well as, encapsulation of an hydrophobic drug. Data on, the influence of the polyphosphate nature of the polymer on drug loading will be presented. [less ▲]

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See detailNitrous oxide emissions using quantum cascade laser spectrometry over a production crop: preliminary results
SALERNO, Giovanni ULg; Aubinet, Marc ULg; Joly, Joly et al

Poster (2013, September 18)

Amongst the greenhouse gases, nitrous oxide is recognized as having the greatest greenhouse forcing potential and as being the third in terms of radiative forcing. Agriculture is known to be the major ... [more ▼]

Amongst the greenhouse gases, nitrous oxide is recognized as having the greatest greenhouse forcing potential and as being the third in terms of radiative forcing. Agriculture is known to be the major anthropic emitter. This work is part of the FERTECOL project and its objective is to measure the emissions of nitrous oxide by a production crop with an eddy covariance system. The measurements extent over the growth period, cover a large range of climatic conditions and capture peak events associated with fertilization. The measurements, started in April 2013, are carried at the Lonzée Terrestrial Observatory (50°33'5.83"N- 4°44'46.22"E). On this year, the crop is planted with winter wheat. A Quantum Cascade Laser Absorption Spectrometer (QCLAS, GSMA, France) is used to measure nitrous oxide as well as water vapor and carbon dioxide at a frequency of 5 hertz. The fluxes are computed using the Eddy Covariance technique adapting standards quality and corrections procedures set up for CO2 fluxes to N2O. Standard meteorological measurements are performed in parallel. The analysis will present response of the fluxes to environmental variables as well as to fertilization events during the season. [less ▲]

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See detailNumerical simulation of the electrical characteristics of CIGS/CdS/ZnO solar cell heterostructures
Amand, Julien ULg; Nguyen, Ngoc Duy ULg

Poster (2013, September 17)

The electrical characteristics of CIGS-based solar cell heterostructure have been simulated by numerically solving the basic semiconductor equations by means of a finite differences method based on a ... [more ▼]

The electrical characteristics of CIGS-based solar cell heterostructure have been simulated by numerically solving the basic semiconductor equations by means of a finite differences method based on a Scharfetter-Gummel discretization scheme. The electric potential, electric field, carrier concentrations, current densities and recombination rates are obtained as function of the space coordinate and the bias voltage. Starting with the analysis of a single absorber layer structure sandwiched between two metal electrodes, we subsequently studied the properties of the CIGS/ZnO pn heterojunction and the influence of the buffer layer thickness in the CIGS/CdS/ZnO on the cell electrical response. A special focus was also given to the influence of grain boundaries in the bulk of CIGS depending on the defects nature and concentration. [less ▲]

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See detailReplication of large-scale epistasis studies: an example on ankylosing spondylitis
Bessonov, Kyrylo ULg; Van Steen, Kristel ULg

Poster (2013, September 17)

Ankylosing spondylitis (AS) is a common form of inflammatory arthritis occurring in approximately 5 out of 1,000 adults of European descent. Recently, the Australo-Anglo-American Spondyloarthritis ... [more ▼]

Ankylosing spondylitis (AS) is a common form of inflammatory arthritis occurring in approximately 5 out of 1,000 adults of European descent. Recently, the Australo-Anglo-American Spondyloarthritis Consortium and the WTCCC2 showed that polymorphisms of ERAP1 only affect AS risk in HLA-B27-positive individuals, hereby establishing an interaction between ERAP1 and HLA in the TASC, WTCCC2 and replication datasets [2,5]. We were able to confirm this interaction although using other SNPs. In this study, we use the aforementioned data from WTTCC2 on AS to address unresolved issues when performing large-scale SNP-SNP interaction studies, so as to better guarantee “stable” and “truly replicable” results. These issues are 1) the choice of variable selection method (e.g., of known loci mapping to genes part of know pathways), 2) the choice of SNPs representing a genomic region (e.g., SNPs with modest versus negligible LD between them), 3) the choice of analysis method (e.g., regression-based versus data-reduction (non-parametric) based), 4) different adjustment schemes for lower-order effects (using additive/co-dominant genetic models). We show that even modest changes in 1)-4) may give rise to quite varying epistasis findings for AS, and motivate some “optimal” choices via extensive simulation studies. In this work we rely on a minimal GWAI protocol for genome-wide epistasis detection using SNPs, as developed in our lab [6][9], using the advanced non-parametric Model-Based Multifactor Dimensionality Reduction (MB-MDR) method [1] and an adapted [*] BOolean Operation-based Screening and Testing (BOOST) algorithm [4]. [*] A BOOST [4] like implementation based on the original BOOST algorithm which accounts for missing genotypes [less ▲]

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See detailClass V β Tubulin during the development of the organ of Corti in rat
Renauld, Justine ULg; Thelen, Nicolas ULg; Johnen, Nicolas ULg et al

Poster (2013, September 13)

In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is ... [more ▼]

In eutherian mammals, the organ responsible for the transduction of sound waves into nerve impulses is called the organ of Corti. This structure located within the cochlea, a portion of the inner ear, is composed by two types of cells: sensory hair cells and non-sensory supporting cells. All these cells are distributed according to a specific arrangement along the whole length of the cochlea. A feature of the organ of Corti’s supporting cell is the presence of an abundant cytoskeleton. This one is mainly composed of microtubules, structures make up by tubulin heterodimers. The heterodimers consist of one molecule of α tubulin and one molecule of β tubulin. β tubulin isotypes are highly conserved in evolution and differ by only a few amino acid residues, implying that the isotypes may have functional significance. Organ of Corti’s supporting cells are constituted by class V β-tubulin, a minor mammalian tubulin (Bhattacharya et al., 2008). Moreover, their microtubules are formed by 15 protofilaments instead of the canonical 13, a unique fact among vertebrates (Banerjee et al., 2008). Such a configuration of protofilaments has been observed in C. elegans’ neurons which are responsible for the mechanosensory sense of touch (Bounoutas et al., 2009). It was also shown that these 15 protofilaments microtubules were essential to the proper functioning of these mechanosensory neurons (Bounoutas et al., 2009). Here we present the spatiotemporal localization of class V β-tubulin during the development of the organ of Corti in rats from embryonic day 18 (E18) until P25 (25th postnatal day). For this purpose, we have used immunolabelings on cryosections of whole cochlea. Our preliminary results demonstrate that class V β-tubulin has a unique distribution in the cochlea, being restricted to supporting cells, especially in pillar cells. [less ▲]

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See detailProteomic study of lumbar spinal cord after quadricipital eccentric exercise
Lacrosse, Zoé ULg; Hody, Stéphanie ULg; Leprince, Pierre ULg et al

Poster (2013, September 12)

Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” ... [more ▼]

Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” (DOMS). DOMS include muscle pain that appears 24 to 72 hours after exercise, but also stiffness, edema and muscle proteins release in plasma as a hallmark of muscle fibers injuries. The only systematic intervention that brings a muscle protection against DOMS is to realize submaximal eccentric contractions with a progressively increased intensity. The mechanism of this protection, called the “Repeated Bout Effect” (RBE), is not understood. However, it is likely explained by cellular, mechanical and neural theories [Scand.J.Med.&Sci.Sports, 13, 88, 2003]. The objective of this study is to better understand which neural signal is released in the muscle synapse and which brings protection by RBE. Male adult mice (C57BL6) were randomly divided into downhill running (DHR), uphill running (UHR) and untrained control (CONT) groups (n=4/group). DHR group is characterized by eccentric contractions of the quadriceps while UHR is concerned by concentric contractions. Running groups performed a warm-up of ten minutes followed by an interval exercise on an inclined treadmill at a velocity of 20cm/s. The latter consisted of running 18 bouts of 5 minutes interspersed with a 2 minutes rest. Lumbar spinal cord was dissected 24h after the race. Nuclear and cytoplasmic proteins were separately extracted and subjected to a 2D-DIGE analysis coupled with mass spectrometry. We do not observe any cytoplasmic protein modification while in the nuclear extract, seven spots were more abundant in eccentric group and four in concentric group in comparison with control group. The mass spectrometry of these proteins reveals that they are implicated in axoplasmic transport. At 24 hours, too few proteins modifications were detected in lumbar spinal cord, maybe as a consequence of a too short period between race and euthanasia. Implication of axoplasmic transport comforts our starting hypothesis that nervous system is able to protect muscle during the RBE by a synthesis and then a synaptic release of molecules modifying the muscle physiology. [less ▲]

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See detailTRAPPIST monitoring of comet C/2012 F6 (Lemmon)
Opitom, Cyrielle ULg; Jehin, Emmanuel ULg; Manfroid, Jean ULg et al

Poster (2013, September 12)

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See detailA water-soluble salt of curcumin (NDS27) inhibits myeloperoxidase and NADPH oxidase activities, two major enzymes of neutrophils.
Derochette, Sandrine ULg; Mouithys-Mickalad, Ange ULg; Deby-Dupont, Ginette et al

Poster (2013, September 11)

Neutrophils (PMNs) produce reactive oxygen species (ROS) to kill pathogenic agents. After appropriate stimulation, leading to the activation of protein kinase C (PKC), the cytosolic subunits of the NADPH ... [more ▼]

Neutrophils (PMNs) produce reactive oxygen species (ROS) to kill pathogenic agents. After appropriate stimulation, leading to the activation of protein kinase C (PKC), the cytosolic subunits of the NADPH oxidase (Nox2) are phosphorylated and translocated to the membrane flavocytochrome b558, forming the active enzyme which produces superoxide anion (O2●-). From O2●- derives H2O2 used by the PMNs myeloperoxidase (MPO) to form strong oxidant species. Many human and animal pathologies with fatal issue are associated with uncontrolled activation of PMNs. The modulation of enzymes implied in ROS production is thus a primary target to manage excessive inflammatory events. For this purpose, we evaluated the effects of NDS27, a water-soluble salt of curcumin combined with hydroxypropyl-β-cyclodextrin, on the activities of PKC, Nox2 and MPO. PKC activation was determined by western blotting with specific antibodies against phosphorylated PKC in extracts from PMNs after their incubation or not with NDS27. A cell-free assay was used to evaluate the effect of NDS27 before or after the assembly of Nox2 subunits. MPO activity was tested by the SIEFED technique in which NDS27 was pre-incubated with the enzyme and discarded before its activity measurement. An inhibition of PKC phosphorylation and Nox2 activity were observed at respectively 10-4 and 10-5 M of NDS27. The Nox2 inhibition was more pronounced when NDS27 was added before the assembly stimulation, suggesting a direct action of NDS27 on the subunits translocation. NDS27 also dose-dependently decreased the activity of MPO (21 % at 10-5 M), indicating an interaction with the enzyme structure. Our results demonstrated that NDS27 is a potent inhibitor of the two major enzymes responsible for ROS production in PMNs, and also acts on the activation cascade of Nox2. The modulatory effect of NDS27 towards the oxidant activity of PMNs opens therapeutic perspectives to control pathologies with excessive inflammatory reactions. [less ▲]

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See detailThe Multicoloured Asian Ladybird, invasive or not in agroecosystems ?
Vandereycken, Axel ULg; Durieux, Delphine ULg; Fassotte, Bérénice ULg et al

Poster (2013, September 10)

The Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) is known to thrive principally in shrubby and arboreal habitats. Its occurrence in agroecosystems remains poorly ... [more ▼]

The Multicoloured Asian Ladybird, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) is known to thrive principally in shrubby and arboreal habitats. Its occurrence in agroecosystems remains poorly documented. This study focuses on the occurrence of this exotic species and its seasonal abundance in various field crops. The abundance of adults and larvae of H. axyridis was evaluated over a four-year period, from 2009 to 2012, in four important agronomical crops (wheat, corn, broad bean and potato) in Belgium. A total of 28 aphid predator species were observed including 14 coccinellid species, 13 hoverfly species and one lacewing species. H. axyridis is present and reproduces in all of the four crops studied, with the largest numbers recorded in corn and broad bean crops. In corn, H. axyridis numbers were found to increase over the four inventoried year, reaching 86% of the aphid predators in 2012, while it represented only 15% in 2009. H. axyridis was not always recorded where aphids were abundant, e.g. aphids were abundant on wheat where no H. axyridis were recorded. H. axyridis starts reproducing after the peak in aphid population, suggesting that H. axyridis is able to complete its development by feeding on alternative prey such as larvae and pupae of the same and other species of ladybird and other aphidophagous species. H. axyridis is often considered to be bivoltine but it only completes one generation per year in field crops. The second generation generally develops late in the season in other habitats. Harmonia axyridis is an invasive and an intraguild predator present in high quantities in some specific crops. In these crops, H. axyridis could negatively impact on population of native species due to IGP observed in several other studies. [less ▲]

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See detailPrevalence and Richness of Gastrointestinal Parasites of Capuchins (Cebus albifrons) Interacting with humans in the Ecuadorian Amazon
Ramirez, William; Martin, Sarah ULg; Carrillo Bilbao, Gabriel Alberto et al

Poster (2013, September 10)

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See detailFree Group and Recognizability
Raskin, Julien ULg

Poster (2013, September 09)

It is well known that recognizability has many algebraic properties. For example, a subset $L$ of the free monoid $\Sigma^*$ is recognizable if and only if there exists a finite monoid $M$, a subset $P ... [more ▼]

It is well known that recognizability has many algebraic properties. For example, a subset $L$ of the free monoid $\Sigma^*$ is recognizable if and only if there exists a finite monoid $M$, a subset $P$ of $M$ and a morphism $f : \Sigma^* \to M$ such that $L = f^{-1}(P)$. These properties allow us to easily define a concept of recognizability in non-free monoids or even in other algebraic structures, such as groups. Our aim is to study the recognizable subsets of the free group $F_X$ generated by $X$. A classical construction of the latter shows that it can be seen as a subset of the free monoid $(X \cup X')^*$, where $X'$ is a set of formal inverses of elements of $X$, endowed with an ad hoc operation. When $X$ is finite, it appears that $F_X$ is a recognizable language of this monoid. It is then natural to wonder if there is a link between recognizability in $F_X$ and recognizability in $(X \cup X')^*$. We show that every recognizable language of $F_X$ is recognizable in $(X \cup X')^*$, and that we can define a class of automata that recognize the recognizable languages of $F_X$. [less ▲]

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See detailMorphology and electrical conductivity of poly(propylene)/multi-walled carbon nanotubes nanocomposites foams compatibilized by poly(propylene) -graft-maleic anhydride (PP-g-MA)
Tran, Minh Phuong ULg; Detrembleur, Christophe ULg; Alexandre, Michaël et al

Poster (2013, September 09)

The agglomerate of carbon nanotubes (CNTs) in poly(propylene) (PP) matrix often results in low electrical conductivity and poor mechanical properties. In order to improve the dispersion of CNTs, different ... [more ▼]

The agglomerate of carbon nanotubes (CNTs) in poly(propylene) (PP) matrix often results in low electrical conductivity and poor mechanical properties. In order to improve the dispersion of CNTs, different amounts of compatibilizer based on poly(propylene-graft-maleic anhydride) (PP-g-MA) were added in the PP matrix. Carbon nanotubes materbatches pre-dispersed at a high loading in the compatibilizer were used to create the samples used in this study. The nanocomposites of PP/PP-g-MA/CNTs were then foamed in supercritical carbon diozide (scCO2) followed by analysis of the foam morphology and the electrical conductivity. The presence of PPgMA did not significantly change the foam morphology, which exhibits good homogeneity and highly uniform closed-cells with penta-heptagonal cell-form. The expansion volume of the foams is not adversely affected by the addition of the compatibilizer; very high expansion volume (around 15 - 25 times) was achieved. The most interesting point is that the PP-PPgMA - 4wt%CNTs foams show a significantly higher electrical conductivity than the uncompatibilized PP-4wt%CNTs at the same volume percent content of CNTs [less ▲]

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See detailClassification of riparian forest species (individual tree level) using UAV-based Canopy Height Model and multi-temporal orthophotos (Vielsalm, Eastern Belgium)
Michez, Adrien ULg; Lisein, Jonathan ULg; Toromanoff, François ULg et al

Poster (2013, September 09)

Introduction : Despite their relatively low area coverage, riparian forests are central landscape features providing several ecosystem services. Nevertheless, they are critically endangered in European ... [more ▼]

Introduction : Despite their relatively low area coverage, riparian forests are central landscape features providing several ecosystem services. Nevertheless, they are critically endangered in European countries by human pressures (livestock grazing, land use conflicts, canalizations, waste water, ...) andalso by natural hazards such as the recent black alder (Alnus glutinosa) extensive decline caused by Phytophthora alni. In this study UAV is used to improve the characterization of riparian areas. Riparian forest species are identified at the individual tree level. The health condition of black alder is assessed. For this purpose a computer based approach has been developped, with low needs of specific operator ability or training. Methods : We used the Gatewing X100 to acquire 16 aerial photographs datasets (7 in classic RGB and 9 in RG NIR) during 5 days (form Augustus to October 2012). We processed a CHM in ArcGIS by combining a national Digital Terrain Model with a photogrammetric DSM generated from a single flight photographs dataset with the "MicMac" opensource platform. The 16 orthophotos were computed with Agisoft Photoscan. Based on the CHM and some basic vegetation index (mean NDVI), a classification/segmentation process was developped in eCognition allowing tree crown extraction. An amount of 113 metrics were computed in eCognition for every tree crown object. The metrics were both derived from the CHM raster and spectral information. Metrics were computed by band (object spectral mean and CHM mean, Harralick entropy, Skewness) but also with band combination (Green NDVI and NDVI). A reference dataset was also acquired through a field survey of 624 individual tree positions accurately localized. The health condition of the black alder was recorded during the field survey. A supervised classification algorithm was developed in R (Random Forest package). Results : Several classification trees were assessed trough global accuracy using the Out Of Bag (OOB) error. The best global accuracy (82%) was obtained when distinguishing the black alder (with no regards for health condition during field survey) from the rest of riparian forest objects. The global accuracy tended to decline when other species were added. When separating healthy black alders from those with symptoms, the global accuracy is 77%. Conclusions : Our study highlights the potential of UAV-based multitemporal orthophotos to identify riparian forest species and health conditions at the tree level. Future studies will focus on quick radiometrics corrections. This could improve global accuracy by reducing the variability caused by illumination conditions [less ▲]

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See detailLinear formulation of identifying cdes in graphs
Vandomme, Elise ULg; Gravier, Sylvain; Parreau, Aline ULg

Poster (2013, September 09)

Identifying codes were introduced by Karpovsky, Chakrabarty and Levitin in 1998 and can be applied to locate fire in a building using sensors. Buildings are modelled by graphs with rooms as vertices. The ... [more ▼]

Identifying codes were introduced by Karpovsky, Chakrabarty and Levitin in 1998 and can be applied to locate fire in a building using sensors. Buildings are modelled by graphs with rooms as vertices. The placement of sensors in the rooms corresponds to choosing a subset of vertices. Finding a sensor-placement such that the location of a fire in one room can be precisely determined is equivalent to constructing an identifying code in the graph. These are dominating sets of vertices for which the closed neighbourhood of each vertex (i.e., the vertex and its neighbours) has a unique intersection with the set. The problem of finding an identifying code has been widely studied. Yet its formulation as an integer linear problem hasn't been much considered. Let G be a graph with vertex set V, to an identifying code $C\subseteq V$ of $G$ correspond weights x_u (x_u is 1 if u belongs to C, otherwise x_u is 0) satisfying the following : for all vertices u,v * the sum of the x_w for w in the closed neighbourhood of u is at least 1 * the sum of the x_w for w in the symmetric difference of the closed neighbourhoods of u and v is at least 1. Of course, it is interesting to find an identifying code with the smallest possible cardinality. But in general this is a NP-hard problem. A way to obtain bounds on the minimal cardinality is to consider the associated linear problem where the weights x_u are fractional. In the case of vertex-transitive graphs, the minimal cardinality for the fractional case can only take two values which depend on the number of vertices, the degree of the graph and the smallest symmetric difference between any two closed neighbourhoods. We show that for an infinite family of graphs the bound is tight and for another another the bound is far too be reached. [less ▲]

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See detailGeneric results in Denjoy-Carleman classes
Esser, Céline ULg

Poster (2013, September 09)

Denjoy-Carleman classes are spaces of smooth functions which satisfy growth conditions on their derivatives. We distinguish the class of ultradi fferentiable functions of Roumieu type and the class of ... [more ▼]

Denjoy-Carleman classes are spaces of smooth functions which satisfy growth conditions on their derivatives. We distinguish the class of ultradi fferentiable functions of Roumieu type and the class of ultradi fferentiable functions of Beurling typ. Endowed with its natural topology, the Beurling class is a Fr échet space. In the poster, we give a condition to have the strict inclusion of a Roumieu class into aBeurling class. We obtain then generic results about the set of functions of a Beurling class which are nowhere in a Roumieu class. Those generic results are obtained from three di fferent points of view: using the Baire category theorem, the notion of prevalence and the notion of lineability. We also study the particular case of Gevrey classes. [less ▲]

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See detailSensitivity of the CaCO3 content of marine sediments to the kinetic expression of CaCO3 dissolution
Schneider, Birgit; Munhoven, Guy ULg; Regenberg, Anke

Poster (2013, September 05)

The preservation of calcite in marine sediments depends on the CaCO3 saturation state of the ambient pore waters and - to a lesser degree - of overlying seawater. A simple sensitivity analysis shows that ... [more ▼]

The preservation of calcite in marine sediments depends on the CaCO3 saturation state of the ambient pore waters and - to a lesser degree - of overlying seawater. A simple sensitivity analysis shows that the two common expressions to quantify the saturation of seawater with respect to CaCO3 (ΔCO3 =[CO3 ]-[CO3 ]sat , Ω = [CO3 ]/[CO3 ]sat ), when used for the calculation of the time dependent rate of CaCO3 dissolution either in the water column or in the sediments, yield very different rates of CaCO3 dissolution. With the help of an ocean biogeochemical model it was found that different kinetic expressions for pelagic CaCO3 dissolution in an ocean acidification scenario result in a wide range of surface to mid-depth calcite fluxes in the open ocean, which has strong implications for particle ballasting, the flux of calcite to the sediments and finally the global carbon cycle. In the present study we employ a marine sediment model that is fed by calcite and other biogeochemical fluxes from the ocean biogeochemical model using different realizations of calcite dissolution kinetics under (1) preindustrial and (2) last glacial maximum background climate conditions to assess the model-data agreement of the calcite content of deep sea sediments. A better understanding of the mechanisms driving calcite dissolution in the ocean is important to assess the time scale of calcite compensation, for example during glacial-interglacial cycles, but also in future greenhouse scenarios. [less ▲]

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See detailThe experience of chronic illness and psychoptathology across life stories : the case of haemophilic boys and depressive hospitalized adolescents
Jadin, Aurore ULg; Gauthier, Jean-Marie ULg; Boulard, Aurore ULg

Poster (2013, September 04)

The aim of this study is to examine and compare how hemophilic adolescents and depressive hospitalized adolescents tell their own stories, how they build their identities in spite of the treatment ... [more ▼]

The aim of this study is to examine and compare how hemophilic adolescents and depressive hospitalized adolescents tell their own stories, how they build their identities in spite of the treatment constraints and how they involve themselves in different relationships. During an individual semi-structured interview, based on Mc Adams’ works, each teenager’s narrative was collected. Various measurements were taken. The discursive analysis and software were used to analyze the data base. First results show hemophilic boys speak about their disease spontaneously but they don’t identify only to it while depressive hospitalized adolescents define themselves exclusively by their mental disorder. Another report is that, opposed to depressive hospitalized adolescents, hemophilic boys invest hobbies, relationships with peers’ as much as non depressive adolescents even if those can feel different and sometimes restrained. [less ▲]

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See detailInfluence of dipole-dipole interactions on superradiance
Damanet, François ULg; Martin, John ULg

Poster (2013, September 02)

Superradiance, known as the cooperative spontaneous emission of a directional light pulse by excited atoms placed in vacuum, has recently regained attention in the context of photon localization [1] and ... [more ▼]

Superradiance, known as the cooperative spontaneous emission of a directional light pulse by excited atoms placed in vacuum, has recently regained attention in the context of photon localization [1] and single photon cooperative emission [2]. The dissipative dynamics of the atoms is known to depend dramatically on the ratio between the typical inter- atomic distance and the atomic transition wavelength, notably because of dipole-dipole interactions [3]. In this work, we study the effects of these interactions on superradiance as in [4] by solving numerically the corresponding master equation. In particular, by averaging over many realizations of the randomly distributed atomic positions, we show that the decay of the radiated energy pulse height with the intensity of the dipolar coupling follows a power law. [1] E. Ackermans, A. Gero & R. Kaiser, Phys. Rev. Lett. 101, 103602 (2008). [2] R. Friedberg & J. T. Manassah, J. Phys. B 43, 035501 (2010). [3] M. Gross & S. Haroche, Physics reports 93, 301-396 (1982). [4] B. Coffey & R. Friedberg, Phys. Rev. A 17, 1033 (1978). [less ▲]

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See detailHighly non-classical symmetric states of an N-qubit system
Baguette, Dorian ULg; Martin, John ULg

Poster (2013, September 02)

In this work, we consider two measures of non-classicality for pure symmetric N-qubit states : Wehrl entropy (S) and Wehrl participation ratio (R). Measures of non-classicality help to the understanding ... [more ▼]

In this work, we consider two measures of non-classicality for pure symmetric N-qubit states : Wehrl entropy (S) and Wehrl participation ratio (R). Measures of non-classicality help to the understanding of the mechanisms responsible for the transition from quantum to classical physics and are usefull in the context of information processing and quantum-enhanced measurements. [less ▲]

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See detailCattle methane fluxes measurement over an intensively grazed grassland using eddy covariance
Dumortier, Pierre ULg; Aubinet, Marc ULg; Beckers, Yves ULg et al

Poster (2013, September)

Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions (European Commission, 2009). Recent technological advances in spectroscopy now ... [more ▼]

Methane emissions account for 8% of the EU-15 GHG emissions and livestock generates approximately half of these emissions (European Commission, 2009). Recent technological advances in spectroscopy now permit methane flux measurement using eddy covariance. This method has numerous strengths. It can measure fluxes in situ, continuously and across broad areas. This provides information about meadow and cattle emission behaviour throughout the year and across a broad range of climatic conditions. We will present here a one year monitoring of methane exchange between an intensively grazed meadow and the atmosphere obtained using the eddy-covariance method. Methane fluxes exchanged by a grazed meadow were measured continuously since June 2012 at the Dorinne Terrestrial Observatory (50˚ 18’ 44” N; 4˚ 58’ 07” E; 248 m asl.) in Belgium. The site is an intensively pastured meadow of 4.2 ha managed according to the regional common practices where up to 30 Belgian Blue cows are grazing simultaneously. Flux measurements were made with the eddy covariance technique, using a fast CH4 analyzer (Picarro G2311-f) and a sonic anemometer (Campbell Csat3). Carbon dioxide fluxes and various micro-meteorological and soil variables, biomass growth and stocking rate evolution were also measured at the site. Turbulent fluxes were calculated according to standard eddy covariance computation schemes and were filtered for non-stationarity and for low friction velocity (u*) events. During grazing periods, fluxes are dominated by the enteric fermentation source and average 111 nmol m-2 s-1. They are highly variable, probably due to cow movements in and out the measurement footprint and cow digestion rhythm. Despite this spread, a daily emission rhythm is observed with higher emissions during the afternoon. When fluxes are integrated over large periods, methane emissions were found strongly related to cattle stocking rate with a slope of 7.34±0.78 mol CH4 day-1 LSU-1. Further developments are ongoing in order to improve cattle geo-localization through infra-red cameras and individual home-made GPS devices. The two systems will be compared in terms of cost, efficiency and ease of use. During cow-free periods, the methane flux averages 10.5 nmol m-2 s-1 and is highly variable with some production peaks above 100 nmol m-2 s-1. No relation was found between methane fluxes and soil temperature while a weak negative relation was found between methane fluxes and soil humidity. No soil methane absorption has been observed. European Commission. Fifth National Communication from the European Community Under the UN Framework Convention on Climate Change (UNFCCC). Technical Report - 2009 – 038 (2009). [less ▲]

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See detailImplementing river restoration taking into account constraints of water supply protection: the case study of the Bocq River at Spontin
Peeters, Alexandre ULg; De le Court, Bernard; Verniers, Gisèle

Poster (2013, September)

In the Bocq basin, eastern tributary of the Meuse, restoration works consist of making 20 obstacles passable to improve the free movement of fish and sediment. This presentation focuses on a particular ... [more ▼]

In the Bocq basin, eastern tributary of the Meuse, restoration works consist of making 20 obstacles passable to improve the free movement of fish and sediment. This presentation focuses on a particular case study involving river restoration in a groundwater abstraction zone. In the sixties, a reach of more than 600 meters of the Bocq was completely channelized in order to avoid any risk of contamination of the nearby important drinking water well field area. Ecological quality was consequently impoverished due to the loss of natural habitats (streambed and banks made of concrete and masonry). In addition, the hydraulic conditions (high flow, low depth) make it totally insurmountable for fish. The restoration project started with a 3-year period of consultation with the water abstraction owner to finally reach an agreement on a restoration project taking into account the need of protection of the water abstraction. The project consisted on building rock weirs at regular interval in order to create a succession of 23 steps and pools. Furthermore, various habitats schemes were implemented such as fish shelter, rock berms for aquatic vegetation, and spawning gravel introduction. In addition a small dike and an expansion area for flooding have been completed to protect the water abstraction. This project is being monitored on the basis of geomorphological and ecological analysis. Geomorphological monitoring focuses on the bedload transport with an analysis of flood and the clogging of the gravel layer. Ecological monitoring is based on two indicators (macroinvertebrates and fishes) as well as the analysis of microhabitats. The first results show an improvement after 1 year. [less ▲]

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See detailHETEROGENOUS CLINICAL AND LABORATORY PRESENTATIONS IN MAD DEFICIENCY
BOEMER, François ULg; SCHOOS, Roland ULg; ACQUAVIVA, Cécile et al

Poster (2013, September)

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See detailAssigned value determination on soil materials throughout the whole analytical field introducing bias correction from reference materials
Planchon, Viviane; Renneson, Malorie ULg; Goffaux, Marie-Julie et al

Poster (2013, September)

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See detailThe Upper Paleolithic of the Ikh Tulberin Gol (Northern Mongolia): new excavation at the Tolbor 16 site
Zwyns, N.; Gladyshev, S.A.; Gunchinsuren, B. et al

Poster (2013, September)

Detailed reference viewed: 13 (2 ULg)
See detailThe Ganymede aurora …
Gérard, Jean-Claude ULg; Shematovich, Valery; Bisikalo, Dmitry et al

Poster (2013, September)

In this Report we present the Monte Carlo model for calculation of oxygen UV and IR emissions due to the electron precipitation in the Ganymede polar regions. These techniques will provide column ... [more ▼]

In this Report we present the Monte Carlo model for calculation of oxygen UV and IR emissions due to the electron precipitation in the Ganymede polar regions. These techniques will provide column densities of atmospheric species at better than or equal to 1 km spatial resolution, and will constrain the amount of some specific compounds from limb scans and during stellar occultation. This investigation also needs characterization of the vertical temperature profile from ground up to about 400 km altitude with ~5 km vertical resolution as well as mapping of water vapour concentration. This can be performed by multiple water line observations in the 200-600 μm wavelength range. It shall be complemented by ion and neutral mass spectrometry of plasma particles, radio occultations to measure structures of the neutral atmosphere and ionosphere, and plasma wave measurements to constrain plasma density and temperature of the ionosphere. [less ▲]

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See detailOptimal conception of a post-combustion CO2 capture unit with assessment of solvent degradation
Léonard, Grégoire ULg; Toye, Dominique ULg; Heyen, Georges ULg

Poster (2013, September)

Solvent degradation may be a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. A kinetics model describing ... [more ▼]

Solvent degradation may be a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. A kinetics model describing solvent oxidative and thermal degradation has been developed based on experimental results. This model has been included into a global Aspen Plus model of the CO2 capture process, so that optimal operating conditions can be identified to minimize both energy and environmental impacts of the process. [less ▲]

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See detailHepatitis E virus infection in wild boars and humans in Belgium
Thiry, Damien ULg; Mauroy, Axel ULg; Saegerman, Claude ULg et al

Poster (2013, September)

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See detailDisruption in energy metabolism and mitochondrial function in a cellular model of inflammation-induced acute kidney injury
Quoilin, Caroline ULg; Mouithys-Mickalad, Ange ULg; Lécart, Sandrine et al

Poster (2013, September)

Sepsis is a very complex clinical condition characterized by stimulation of a systemic inflammatory response due to an infection. It has a profound deleterious effect on kidney functions leading to sepsis ... [more ▼]

Sepsis is a very complex clinical condition characterized by stimulation of a systemic inflammatory response due to an infection. It has a profound deleterious effect on kidney functions leading to sepsis-induced acute kidney injury (AKI). This failure seems to occur through complex mechanisms involving the immune system response, inflammatory pathways, cellular dysfunction and hemodynamic instability. To study the role of cellular energetic metabolism dysfunction and mitochondrial impairment in the occurrence of AKI during sepsis, we developed an inflammation-induced in vitro model using proximal tubular epithelial cells (HK-2) exposed to a bacterial endotoxin (lipopolysaccharide, LPS). This investigation has provided key features on the relationship between endotoxic stress and mitochondrial respiratory chain assembly defects. Firstly, we have shown that renal cells subjected to LPS are no longer capable to use adequately the available oxygen to maintain their metabolic functions. One hypothesis of this down-regulation suggests that impairment in mitochondria oxidative phosphorylation could prevent cells from using oxygen for adenosine triphosphate (ATP) production and potentially could cause sepsis-induced organ failure. Our study has then investigated this possible mitochondrial impairment to explain the decreased O2 consumption rate observed in LPS-treated HK-2 cells. After exposure to LPS, functionality of mitochondria was affected without any disturbance in their spatial organization. LPS seemed rather to interrupt mitochondrial oxidative phosphorylation by blocking cytochrome c oxidase activity. As a consequence, disruptions in the electron transport and the proton pumping across the system occurred, leading to a decrease of the mitochondrial membrane potential, an electron leakage as the form of superoxide anion, a release of cytochrome c in the cytosol and a decrease in ATP production. This irreversible defect in the production of cellular energy would support the concept that kidney failure in sepsis may occur on the basis of cytopathic hypoxia. [less ▲]

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See detailErosion littorale et migrations forcées de réfugiés environnementaux. L'exemple de Cotonou, Bénin
Ozer, Pierre ULg; Hountondji, Yvon-Carmen; De Longueville, Florence ULg

Poster (2013, September)

Situé dans le Golfe de Guinée, le littoral béninois est soumis –sur certains tronçons– à une érosion assez rapide ces dernières décennies. Cette érosion côtière est principalement due actuellement aux ... [more ▼]

Situé dans le Golfe de Guinée, le littoral béninois est soumis –sur certains tronçons– à une érosion assez rapide ces dernières décennies. Cette érosion côtière est principalement due actuellement aux activités humaines parmi lesquelles les perturbations sédimentaires occasionnées par la construction de divers barrages dont celui de Nangbéto sur le fleuve Mono ; le blocage du transit littoral par les ouvrages portuaires de Cotonou ; les carrières de sable exploitées à même la plage ; et la diminution d’apports sédimentaires provenant de l’ouest suite à divers travaux de protection des côtes. En utilisant la fonction multi dates disponible dans Google Earth, cet article estime la superficie érodée à Cotonou entre 2002 et 2011 à l’est de l’exutoire du Lac Nokoué. En outre, il évalue le nombre de maisons détruites ainsi que le nombre de personnes contraintes à une migration forcée. Les figures sont importantes puisque de l’ordre de cent mètres de zone côtière ont totalement disparu au cours des dix dernières années sur un tronçon de près de six kilomètres de long en pleine ville ou en proche périphérie. Cette analyse montre qu’actuellement les dommageables modifications géomorphologiques résultent essentiellement de l’addition non envisagée d’activités humaines couplée à l’absence de gouvernance. Par ailleurs, nous sommes en droit de nous interroger sur les risques d’érosion côtière dans les décennies à venir avec l’amplification annoncée de l’augmentation du niveau des océans due au réchauffement climatique. [less ▲]

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See detailInsulin Sensitivity during Hypothermia in Critically Ill Patients
Sah Pri, Azurahisham; Chase, J. Geoffrey; Le Compte, Aaron J. et al

Poster (2013, September)

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See detailNegative effect of milk production level on reproduction performances. Not a fatality.
Hanzen, Christian ULg; Glorieux, Géry; Chapaux, Philippe

Poster (2013, September)

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See detailMagnetic hysteresis cycle and remnant field distribution of bulk high temperature superconductor / ferromagnet hybrids
Philippe, Matthieu ULg; Fagnard, Jean-François ULg; Wera, Laurent ULg et al

Poster (2013, September)

Bulk (RE)BCO materials can be used potentially as powerful permanent magnets. The magnetic flux distribution above a bulk HTS magnet, however, is strongly non-uniform (conic profile predicted by the Bean ... [more ▼]

Bulk (RE)BCO materials can be used potentially as powerful permanent magnets. The magnetic flux distribution above a bulk HTS magnet, however, is strongly non-uniform (conic profile predicted by the Bean model) compared to the rather flat distribution above a ferromagnet. In the present work, we study how FeNi soft ferromagnetic alloys of different shapes can be combined with a bulk, large grain (RE)BCO superconductor (RE denotes a rare-earth element) to improve the distribution of trapped field at the surface or its average value through the volume of the sample. A FeNi ferromagnetic alloy was machined into pieces of various shapes (cylinders and rings) and attached to (i) the top surface of the bulk HTS cylinder to form bulk ferromagnet / superconductor (F/S) hybrids and (ii) to the top and bottom surfaces to form bulk F/S/F hybrids. The magnetic properties of each hybrid structure were measured under axial magnetic field at 77 K. Pick-up coils wound around the superconductor were used to measure the average magnetic induction inside the superconductor while the remnant induction distribution near the top and bottom surfaces was determined by miniature Hall probe mapping. The modifications of the hysteresis curves and flux distributions were analyzed by taking into account the ferromagnet intrinsic properties (intrinsic permeability, saturation) and geometrical properties (shape, size and volume). The results show that the effect of the ferromagnet increases with its volume. In presence of a ferromagnet, the superconductor hysteresis curve shows a combination of a diamagnetic and a ferromagnetic behaviour on which it is worth noting that (i) the bulk remnant magnetization increases and (ii) in the magnetic saturation regime of the ferromagnet, the magnetic effects of the superconductor and the ferromagnet are superimposed. The results also give evidence that flux lines curve through the ferromagnetic component, which produces a decrease of the self-demagnetizing field inside the superconductor. [less ▲]

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See detailImpact of grazing on carbon dioxide flux exchanges in an intensively managed grassland
Jerome, Elisabeth ULg; Beckers, Yves ULg; Bodson, Bernard ULg et al

Poster (2013, September)

To date, there are few studies assessing the impact of specific management events, particularly grazing, on carbon (C) and carbon dioxide (CO2) fluxes in managed grasslands. Grazing effects are indeed ... [more ▼]

To date, there are few studies assessing the impact of specific management events, particularly grazing, on carbon (C) and carbon dioxide (CO2) fluxes in managed grasslands. Grazing effects are indeed difficult to discern. They vary with the stocking rate and the length of the grazing period. Moreover, they are often masked by environmental responses. The aim of the present study was to assess the impact of grazing on the CO2 fluxes of a grassland grazed by the Belgian Blue breed of cattle. The research was run at the Dorinne Terrestrial Observatory (DTO), located in the Belgian Condroz (50° 18’ 44’’ N; 4° 58’ 07’’ E; 248 m asl.). The site is a permanent grassland of ca. 4.2 ha subjected to intensive management. Grassland carbon budget at the system boundaries is calculated from Net Ecosystem Exchange of CO2 measured by eddy covariance by taking imports and exports of organic C and losses of carbon as methane into account. After 2 years of measurements, the site was close to equilibrium. If management practices (harvest, fertilization and imports as supplementary feedings) and climatic conditions had a significant impact on the C balance, the impact of grazing was uncertain, especially on CO2 fluxes. To do this analysis, we distinguished the long term and the short term impacts of grazing on CO2 fluxes. The long term effect results from the biomass consummation by the cattle and from the cattle effluents that modify assimilation and respiration fluxes. This could be quantified only by comparing fluxes before and after grazing periods. The short term impact is due to cattle respiration that is a part of total ecosystem respiration and should be measured in its presence in the field. For the long term effects of grazing on CO2 fluxes, we analyzed the temporal evolution of gross maximal photosynthetic capacity GPPmax and dark respiration normalized at 10°C (Rd,10). Those parameters were deduced from the response of daytime CO2 fluxes to radiation over 5-day windows. We calculated parameters variations between the beginning and the end of grazing and non-grazing periods (∆GPPmax, ∆Rd,10) and analyzed their dependence to stocking rate. We found a significant decreased of ∆GPPmax that allowed us to quantify the assimilation reduction due to grass consumption by cattle. Discrimination of this impact from flux response to climate was possible only after gathering and treating two years of measurements taken under various climatic conditions. At the opposite, no significant evolution of Rd,10 with the average stocking rate was found. The short term impacts were an increase of CO2 fluxes in presence of cattle. It could be distinguished and quantified only thanks to confinement experiments. Each experiment extended over two days: the first day, cattle was confined in the footprint of the eddy covariance set-up (1.76 ha, 27 LU ha-1) and the second day, it was removed from it. We compared filtered half-hourly data made at 24h intervals, in the presence or absence of cattle, considering that environmental conditions were equivalent (air temperature, wind speed, radiation and wind direction). Livestock contribution to CO2 fluxes was estimated to be 2.25 ± 0.68 kg C LU-1 d-1. [less ▲]

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See detailHow can long-term experimental plots can help us to understand the sustainability of different phosphorus inputs ?
Renneson, Malorie ULg; Dufey, Joseph; Roisin, Christian et al

Poster (2013, September)

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See detailX-ray emission of interacting wind binaries in Cyg OB2
Nazé, Yaël ULg; Cazorla, Constantin ULg; Rauw, Grégor ULg

Poster (2013, September)

Cyg OB2 #5, #8A, and #9 are binary or multiple massive stars in the Cyg OB2 association displaying several peculiarities, such as bright X-ray emission and non-thermal radio emission. Our X-ray monitoring ... [more ▼]

Cyg OB2 #5, #8A, and #9 are binary or multiple massive stars in the Cyg OB2 association displaying several peculiarities, such as bright X-ray emission and non-thermal radio emission. Our X-ray monitoring of these stars reveals the details of their behaviours at high energies, which can be directly linked to wind-wind collisions (WWCs). In addition, the X-ray emission of Cyg OB2 #12, an evolved massive star, shows a long-term decrease, which could hint at the presence of a companion (with associated colliding winds) or indicate the return to quiescence of the system following a recent eruption. [less ▲]

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See detailInteractions of two aphid species on the african eggplant, sorrel and amaranth
Bayendi-Loudit, Sandrine ULg; Chevalier Mendes Lopes, Thomas ULg; Francis, Frédéric ULg

Poster (2013, September)

Aphids (Homoptera: Aphididae) are polyphagous insects which can be found on several crops in temperate zones, as well as in the tropics. The multiplication of Aphis gossypii Glover (C9 cucumber, Burk ... [more ▼]

Aphids (Homoptera: Aphididae) are polyphagous insects which can be found on several crops in temperate zones, as well as in the tropics. The multiplication of Aphis gossypii Glover (C9 cucumber, Burk cotton and Pipo pepper strains) and Myzus persicae Sulzer was studied in the laboratory on three plant species: African eggplant, Solanum aethiopicum, sorrel, Hibiscus sabdariffa, and amaranth, Amaranthus spp. Periodic counts were carried out to monitor population growth. The multiplication rate of M. persicae wass higher than that of A. gossypii when these two species were present together on eggplant. Amaranth was less suitable for the development of both species, but Myzus persicae again had a better multiplication rate than Aphis gossypii. This study illustrated the importance of considering not only one pest species, but the whole herbivore guild, especially when biological control is important. [less ▲]

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See detailTiO2 templated films used as photoelectrode for solid-state DSSC applications: study of the pore filling by Rutherford Backscattering Spectroscopy
Dewalque, Jennifer ULg; Colson, Pierre ULg; Thalluri, Venkata Visveswara Gopala Kris ULg et al

Poster (2013, September)

Liquid-state dye-sensitized solar cells can suffer from electrolyte evaporation and leakage. Therefore solid-state hole transporting materials are investigated as alternative electrolyte materials ... [more ▼]

Liquid-state dye-sensitized solar cells can suffer from electrolyte evaporation and leakage. Therefore solid-state hole transporting materials are investigated as alternative electrolyte materials. However, in solid-state dye-sensitized solar cells, optimal TiO2 films thickness is limited to a few microns allowing the adsorption of only a low quantity of photoactive dye and thus leading to poor light harvesting and low conversion efficiency. In order to overcome this limitation, high surface area templated films are investigated as alternative to nanocrystalline films prepared by doctor-blade or screen-printing. Moreover, templating is expected to improve the pore accessibility what would promote the solid electrolyte penetration inside the porous network, making possible efficient charge transfers. In this study, films prepared from different structuring agents are discussed in terms of microstructural properties (porosity, crystallinity) as well as effect on the dye loading and Spiro-OMeTAD (2,2',7,7'-tetrakis-(N,N-di-p-methoxyphenylamine)9,9'-spirobifluorene) solid electrolyte filling. Different techniques such as transmission electron microscopy (TEM), atmospheric poroellipsometry (AEP) and UV-visible absorption spectroscopy (UV-vis.) have been used to describe the microstructural features of the films. Besides, we have implemented Rutherford backscattering spectrometry (RBS) as an innovative non-destructive tool to characterize the hole transporting materials infiltration. Templated films show dye loading more than two times higher than nanocrystalline films prepared by doctor-blade or screen-printing and solid electrolyte infiltration up to 88%. [less ▲]

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See detailPIXE Analysis for the pigment identification in the Nizet manucript (18th century)
Machowski, Mélanie ULg; Calvo Del Castillo, Helena ULg; Oger, Cécile ULg et al

Poster (2013, September)

Written in 1740, the Nizet Manuscript is a heraldry book compiling the genealogy of the Nizet family (Verviers, Belgium). It presents a large number of hand-painted heraldries in traditional heraldic ... [more ▼]

Written in 1740, the Nizet Manuscript is a heraldry book compiling the genealogy of the Nizet family (Verviers, Belgium). It presents a large number of hand-painted heraldries in traditional heraldic colours; the gold- and silver-like colours have undergone alteration and induced the degradation of the paper. The first inspection of the book with a binocular lens and UV-visible spectroscopy has led to the selection of representative points to be studied by PIXE analysis for the different groups of pigments. The PIXE measurements have been conducted with the cyclotron of the Institute of Nuclear and Atomic Physics and of Spectrometry of the University of Liege. Some pigments have been clearly identified, such as vermillion, umber or brass, while the presence of some others needs to be confirmed, for example azurite or lapis-lazuli. In the case of the green pigments and the organic ones (red, pink and black colours), the PIXE results must be completed by a molecular analysis. In order to exactly identify all the pigments, Raman analyses will be done to complete the pigment identification of the Nizet Manuscript. [less ▲]

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See detailMultifractality of quantum wave functions
Dubertrand, Rémy; Garcia-Mata, Ignacio; Georgeot, Bertrand et al

Poster (2013, September)

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See detailEvaluation of a new rapid test for the detection of norovirus antigen in comparison with Real Time RT-PCR
HUYNEN, Pascale ULg; Mauroy, Axel ULg; Gérard, Catherine ULg et al

Poster (2013, September)

Diagnosis of NoV infection mainly relies on molecular methods. A detection of viral antigens can also be performed by immunochromatographic assays, and may be useful in outbreak settings. The aim of this ... [more ▼]

Diagnosis of NoV infection mainly relies on molecular methods. A detection of viral antigens can also be performed by immunochromatographic assays, and may be useful in outbreak settings. The aim of this study was to compare the performances of the new RDT ImmunoCardSTAT!®Norovirus (Meridian Bioscience®, Europe) with a real time RT-PCR. [less ▲]

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See detailPersistent right aortic arch associated with an aberrant left subclavian artery arising from a patent ductus arteriosus in a puppy
Rizza, Maïlis ULg; Claeys, Stéphanie ULg; Billen, Frédéric ULg et al

Poster (2013, August 31)

PERSISTENT RIGHT AORTIC ARCH ASSOCIATED WITH AN ABERRANT LEFT SUBCLAVIAN ARTERY ARISING FROM A PATENT DUCTUS ARTERIOSUS IN A PUPPY Rizza M.*, Claeys S.**, Billen F.***, Mc Entee K. ***, Bolen G ... [more ▼]

PERSISTENT RIGHT AORTIC ARCH ASSOCIATED WITH AN ABERRANT LEFT SUBCLAVIAN ARTERY ARISING FROM A PATENT DUCTUS ARTERIOSUS IN A PUPPY Rizza M.*, Claeys S.**, Billen F.***, Mc Entee K. ***, Bolen G.* *Diagnostic Imaging Section, **Small Animal Surgery Section, ***Small Animal Internal Medicine Section, Department of Clinical Sciences (Companion Animal and Equides), Faculty of Veterinary Medicine, University of Liege, Belgium. Introduction Vascular anomalies develop during foetal development and single or multiple aberrant vessels can be present. Persistent right aorta arch (PRAA) represents over 90% of described vascular ring anomalies and can be accompanied by a left ligamentum arteriosum or a patent left ductus arteriosus (approximately 10% of patients) that causes oesophageal entrapment and secondary sub-obstruction. Radiography is an effective means of detecting vascular ring anomalies when oesophageal dilation and left-sided tracheal displacement are visible. Computed tomography angiography (CTA) is necessary to confirm the nature and the patency of the vascular anomalies present and to identify which of the identified anomalies is causing the clinical signs. Materials and methods A 3.5-month-old, male French Bulldog was presented for regurgitation and vomiting since weaning. Besides a low body score, physical exam was unremarkable. Hypoglycemia was observed on routine blood analysis. Thoracic radiographs and CTA of the thorax were performed. Results The radiographs revealed severe oesophageal dilation cranial to the base of the heart and a ventral and left-sided tracheal displacement. A congenital oesophageal diverticulum secondary to a vascular anomaly was suspected. CTA showed multiple vascular anomalies. A PRAA was observed. An aberrant right subclavian artery (ARSA) was identified originating from the PRAA next to the brachiocephalic trunk. A patent left-to-right patent ductus arteriosus (PDA) was detected between the aorta and the pulmonary trunk and an aberrant left subclavian artery (ALSA) originated from the PDA. The oesophagus was compressed between the PDA and the trachea and was dilated cranially to this narrowing. Endoscopy revealed severely esophageal distension cranially to an extraluminal stenosis. Surgery was performed to ligate and cut the PDA. Discussion Seven types of vascular ring anomaly are described: types I - III have a PRAA, type IV has a double aortic arch, and types V -VII have a left aortic arch with combinations of persistent right ligamentum arteriosum and right subclavian arteries. In the patient described here, the vascular ring anomalies are a novel variant of the defined types. To the authors’ knowledge, an ALSA originating from a PDA has not been described previously. The severe compression of the oesophagus with severe dilation cranial to the heart was caused by the PDA and was resolved by surgical intervention. In conclusion, CTA is necessary to determine which vascular anomalies are present and to identify which of these anomalies is responsible for the clinical signs. This technique enables accurate pre-operative planning. Pownder S. Scrivani PV. Non-selective computed tomography angiography of a vascular ring anomaly in a dog. J Vet Cardiol. 2008 Dec;10(2):125-8 Henjes CR, Nolte I, Wefstaedt P. Multidetector-row computed tomography of thoracic aortic anomalies in dogs and cats: patent ductus arteriosus and vascular rings. BMC Vet Res. 2011 Sep 23;7:57 [less ▲]

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See detailA Gene Regulatory Network Model to Assess the Stability of the Cartilage Phenotype
Kerkhofs, Johan ULg

Poster (2013, August 29)

Introduction Chondrocyte hypertrophy entails the switching of a genetic program driven by Sox9 to one under control of the osteoblast master regulator Runx2. The switch is a prerequisite step in the bone ... [more ▼]

Introduction Chondrocyte hypertrophy entails the switching of a genetic program driven by Sox9 to one under control of the osteoblast master regulator Runx2. The switch is a prerequisite step in the bone forming process (endochondral ossification) during development and in postnatal fracture repair of larger bone defects. However, this switch can also be detrimental in tissue engineered cartilage constructs and in osteoarthritis development [Saito, 2010]. Therefore, a detailed model of the pathways that can facilitate, or inhibit, this phenotypic switch will lead to a more profound understanding of these processes and provide hints as to how to manipulate them. Methods The model formalism accommodates the qualitative information that is typically available in developmental studies. The literature based network comprises 46 nodes and 161 interactions, shown to be important in endochondral ossification. To simulate network dynamics in discrete time the normalized value of each gene is determined by additive functions where all interactions are assumed to be equally powerful. Furthermore, each species is represented by a fast variable (activity level, as determined by post translation modifications) which is assumed to be in equilibrium with a slow variable (mRNA) at all times. Through a Monte Carlo approach the importance of each node in the stability of chondrocytic phenotypes (proliferating, hypertrophic) is assessed in random initial conditions. A perturbation analysis of the stable states is used to determine the transition likelihood between them as a second measure of stability. Results Both measures of stability indicate that the hypertrophic (Runx2 driven) state is more stable than the proliferating one driven by Sox9. The results for the second measure are given in Fig.1. This higher stability seems to be partly conferred by faster reactions that favour the hypertrophic phenotype. In addition, the results point out that some transcription factors are necessary for the induction of a certain phenotype, whereas other transcription factors are required to maintain the phenotype, but are not necessary capable of inducing it. Discussion These results may relate to the difficulty experienced by researchers in maintaining a stable cartilage phenotype in culture and the occurrence of ectopic hypertrophy in osteoarthritis. By analysing the effect of changes to individual nodes, strategies to stabilise the proliferating phenotype can be developed. Overall, the model allows the importance of several important factors in the fate decision of mesenchymal cells to be quantitatively assessed based mainly on topological information. [less ▲]

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See detailNi/Al2O3 xerogel catalysts for biogas cleaning
Claude, Vincent ULg; Heinrichs, Benoît ULg; Lambert, Stéphanie ULg

Poster (2013, August 29)

This poster summarize the firsts results of the PhD project started on October 2012 about the catalytic purification of biogas. The aim of this project consists in the development of Ni/Al2O3 catalyst in ... [more ▼]

This poster summarize the firsts results of the PhD project started on October 2012 about the catalytic purification of biogas. The aim of this project consists in the development of Ni/Al2O3 catalyst in order to reform the tars present in the outlet gas of biomass gasifier. In order to obtain catalysts with high performance and lifetime, materials need to have optimized specific surface and metal particle dispersion. This poster investigate the effect of different surfactants (Pluronic P123; F127; stéarique acide, EDAS) on the materials properties. [less ▲]

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See detailTime-history of the gravel sheet in Ardennian rivers over the last 100,000 years
Houbrechts, Geoffrey ULg; Denis, Anne-Cécile ULg; Juvigné, Etienne ULg et al

Poster (2013, August 27)

It is generally held that, in north-western Europe, the main part of the gravel sheets under river beds were deposited during the Weichselian period in a periglacial environment. However, other parameters ... [more ▼]

It is generally held that, in north-western Europe, the main part of the gravel sheets under river beds were deposited during the Weichselian period in a periglacial environment. However, other parameters such as propagation of knickpoints in fluvial networks may also influence incision or aggradation. However, only few studies have dated the periods of formation of the gravel sheets and have described their properties. The first aim of this research was to determine the thickness of the gravel sheets still remaining under the river beds and to estimate the potential incision of these rivers before reaching the bedrock. Then we tried to answer a number of other questions: When did these thick gravel deposits fill the valley bottom? When were the lowest terraces abandoned? When did the rivers incise the bedrock? What is the morphology of the bedrock under the gravel layer? Numerous boreholes were made by percussion drilling in different floodplains of the Ardenne Massif and core samples were taken, down to the bedrock. Afterwards, different volcanic tephra from the Late Pleistocene were used as stratigraphic markers to date the relative periods of terrace formation and to reconstruct the past evolution of the gravel sheets. Pollen and metallurgic slag were also used to date the periods of bed level evolution. In the Ardennian massif, the thickness of the gravel sheet beneath the river beds is very variable (from 10 m in the downstream part of the Ourthe River to less than 1 m in the upper catchments). In some valleys, weathered bedrock has been observed under the gravel sheet to a thickness of several meters. Different phases of accumulation and incision over the last 100,000 years have been dated. Some evolutions can be clearly linked to climate changes but some modifications of bed levels also occurred during the Weichselian period and could be a response to the propagation of knickpoints in the fluvial networks. [less ▲]

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See detailHerd-test-day variability of methane emissions predicted from milk MIR spectra in Holstein cows
Vanrobays, Marie-Laure ULg; Kandel, Purna Bhadra ULg; Soyeurt, Hélène ULg et al

Poster (2013, August 26)

The aim of this study was to estimate the herd-test-day (HTD) effect on milk yield, fat and protein content, and methane (CH4) emissions of Walloon Holstein first-parity cows. A total of 412,520 test-day ... [more ▼]

The aim of this study was to estimate the herd-test-day (HTD) effect on milk yield, fat and protein content, and methane (CH4) emissions of Walloon Holstein first-parity cows. A total of 412,520 test-day records and milk mid-infrared (MIR) spectra of 69,223 cows in 1,104 herds were included in the data set. The prediction equation developed by Vanlierde et al. (Abstract submitted to EAAP 2013; R² of cross-validation=0.70) was applied on the recorded spectral data to predict CH4 emissions (g/d). Daily CH4 emissions expressed in g/kg of milk were computed by dividing CH4 emissions (g/d) by daily milk yield of cows. Several bivariate (a CH4 trait with a production trait) random regression test-day models including HTD and classes of days in milk and age at calving as fixed effects and permanent environment and genetic as random effects were used. HTD solutions of studied traits obtained from these models were studied and presented large deviations (CV=17.54%, 8.93%, 4.68%, 15.51%, and 23.18% for milk yield, fat and protein content, MIR CH4 (g/d), and MIR CH4 (g/kg of milk), respectively) indicating differences among herds, especially for milk yield and CH4 traits. HTD means per month of milk yield and fat and protein contents presented similar patterns within year. The maximum of monthly HTD means corresponded to the spring (pastern release) for milk yield and to the winter for fat and protein contents. The minimum corresponded to the month of November for milk yield and to the summer for the other traits. For MIR CH4 (g/d), monthly HTD means showed similar patterns as fat and protein content within year. MIR CH4 (g/kg of milk) presented maximum values of monthly HTD means in November and minimum values in May. Finally, results of this study showed that HTD effects on milk production traits and on MIR CH4 emissions varied through herds and seasons. [less ▲]

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See detailEstimation of dominance variance with sire-dam subclass effects in a crossbred population of pigs
Dufrasne, Marie ULg; Faux, Pierre ULg; Piedboeuf, Maureen et al

Poster (2013, August 26)

Nonadditive genetic effects may be not negligible but are often ignored in genetic evaluations. The most important nonadditive effect is probably dominance. Prediction of dominance effects should allow a ... [more ▼]

Nonadditive genetic effects may be not negligible but are often ignored in genetic evaluations. The most important nonadditive effect is probably dominance. Prediction of dominance effects should allow a more precise estimation of the total genetic merit, particularly in populations that use specialized sire and dam lines, and with large number of full-sibs, like pigs. Computation of the inverted dominance relationship matrix, D-1, is difficult with large datasets. But, D-1 can be replaced by the inverted sire-dam subclass relationship matrix F-1, which represents the average dominance effect of full-sibs. The aim of this study was to estimate dominance variance for longitudinal measurements of body weight (BW) in a crossbred population of pigs, assuming unrelated sire-dam subclass effects. The edited dataset consisted of 20,120 BW measurements recorded between 50 and 210 d of age on 2,341 crossbred pigs from 89 Piétrain sires and 169 Landrace dams. A random regression model was used to estimate variance components. Fixed effects were sex and date of recording. Random effects were additive genetic, permanent environment, sire-dam subclass and residual. Random effects, except residual, were modeled with linear splines. Only full-sib contributions were considered by using uncorrelated sire-dam classes. Estimated heritability of BW increased with age from 0.40 to 0.60. Inversely, estimated dominance decreased with age, from 0.28 to 0.01. Ratio of dominance relative to additive variance was high at early age (58.3% at 50 d) and decreased with age (2.6% at 200 d). Those results showed that dominance effects might be important for early growth traits in pigs. However, this need to be confirmed and dominance relationships will be included in the next steps. [less ▲]

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See detailDifferences in locomotor activity in two syntopic spadefoot toad species (genus Pelobates)
Székely, Diana; Denoël, Mathieu ULg; Székely, Paul et al

Poster (2013, August 25)

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See detailOpal-CT precipitation in a clayey soil explained by geochemical transport model of dissolved Si (Blégny, Belgium)
Ronchi, Benedicta; Barao, A.L.; Vandevenne, F. et al

Poster (2013, August 25)

Opal-CT precipitation controlling dissolved Si export Dissolved Si (DSi) exported by rivers are controlled by geological, hydrological and biological cycle processes [1]. The DSi concentrations measured ... [more ▼]

Opal-CT precipitation controlling dissolved Si export Dissolved Si (DSi) exported by rivers are controlled by geological, hydrological and biological cycle processes [1]. The DSi concentrations measured in a river of an upstream catchment in eastern Belgium (Blégny, Land of Herve) don’t vary seasonally (6.91±0.94mgL-1; n=363). Si concentrations in pore water are often higher and vary more (8.65±3.65mgL-1; n=128). The decrease of DSi along the flowpath of water is due to sink processes, i.e. precipitation, adsorption or uptake by vegetation. As the DSi in the river does not show any seasonal variation, uptake by vegetation can be ruled out [1] whereas precipitation or adsorption can control the DSi drained by the stream water. This hypothesis is confirmed by XRD and DeMaster analysis. At 0.1m depth the soil is constituted of 62% quartz, 7% K-feldspar, 6% plagioclase, 3.2% carbonates, 18.9% Al-clay, 1.47% Kaolinite, 0.63% Chlorite and 0.2% amorphous Si, probably of biogenic origin. At 1.5m depth, the amounts of several minerals (35.8% quartz, 0.6% K-feldspars, 0.9% plagioclase, Al-clay 14.7%) drop drastically. Carbonates, chlorite and kaolinite are absent whereas 40.4% opal-CT appears. The precipitation of opal-CT controls the DSi export of this catchment. Development of geochemical transport model To descripe DSi export from a catchment a geochemical transport model is developped in HP1 which couples the water flux model Hydrus with the geochemical model PHREEQC [2]. Our model is based on the conceptual model developped in [3]. First results show different DSi export dynamics in the unsaturated zone than in the aquifer due to different pCO2 values and varying soil moisture conditions. Further development of the model will help to find out the reason of opal-CT precipitation in this setting. [1]Fulweiler, Nixon (2005) Biogeochemistry 74:115–130. [2] Simunek, Jacques, van Genuchten, Mallants (2006) JAWRA 42:1537-1547. [3] Ronchi et al. (2013). Silicon, 5(1), 115–133. [less ▲]

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See detailInfluence of breed and previous storage time on color and lipid stability of beef packaged in high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Tahiri, Assia ULg; Thimister, Jacqueline ULg et al

Poster (2013, August 20)

The aim of this study was to compare the effect of two breeds (Belgian Blue vs. Limousin) and previous vacuum storage time on color and lipid stability of meat packaged in high-oxygen atmosphere. Vacuum ... [more ▼]

The aim of this study was to compare the effect of two breeds (Belgian Blue vs. Limousin) and previous vacuum storage time on color and lipid stability of meat packaged in high-oxygen atmosphere. Vacuum packaged striploins from Belgian Blue and Limousin cows were stored at −1 °C and +4 °C for up to 60 days and analyzed. Part of these samples were repackaged under modified atmosphere – 70 % O2/30 % CO2 – at different times, stored 2 days at +4 °C and 5 d at +8 °C, and then analyzed. The following parameters were evaluated: color (CIE L*a*b*), metmyoglobin %, lipid oxidation (TBARS) and fat content. Color measurement and metmyoglobin % determination showed greater pigment stability in Belgian Blue samples than in Limousin. Belgian Blue also presented higher lipid stability (TBARS). A positive correlation between pigment oxidation and lipid oxidation was highlighted. The greater amount of fat in meat of Limousin could partially explain its higher sensitivity to oxidation. Nevertheless, other factors may be involved in oxidative stability such as metmyoglobin reducing activity and antioxidant capacity. An understanding of the oxidative processes and their interaction would provide a basis for explaining quality deterioration in meat and for developing strategies to maintain sensory qualities. [less ▲]

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See detailDevice-based controlled local delivery for the treatment of peritoneal pathologies
Riva, Raphaël ULg; Krier, Fabrice; Defrère, Sylvie et al

Poster (2013, August 18)

This contribution aims at reporting the developpment of a controlled drug delivery system (DDS) dedicated to the treatment of intra-peritoneal pathologies, especially endometriosis. At present time ... [more ▼]

This contribution aims at reporting the developpment of a controlled drug delivery system (DDS) dedicated to the treatment of intra-peritoneal pathologies, especially endometriosis. At present time, endometriosis is generally treated by daily oral absorption of drug with the purpose to improve the life quality of patients by the reduction of the pain caused by endometrial lesions. Nevertheless, deleterious side-effects, mainly infertility, are observed as a consequence of the important amount of absorbed active principle. One main advantage of controlled drug delivery devices, e.g. polymer implants, is to maintain sustained drug release over a prolonged period of time thereby eliminating fluctuations in the drug plasma concentration. Moreover, DDS allows a local release of the drug at a specific area, which significantly decreases the active principle concentration in the body and limits side-effects. The peritoneal cavity is a convenient site for the implantation of a DDS against endometriosis because large parts of lesion are localized in this region. At our knowledge, no application of an implant dedicated to the treatment of endometriosis is reported in the literature, whereas the local controlled release of an active principle presents several advantages compared to systemic administration. In this study, anastrozole (2,2’-[5-1H-1,2,4-triazole-1-yl-methyl)-1,3-phenylene]bis(2-methylpropiononitrile)), a well-known aromatase-inhibiting drug, was selected as active molecule. Typically, two non-biodegradable polymers were tested for the elaboration of an anastrozole loaded intra-peritoneal implant, namely polydimethylsiloxane (PDMS) and poly(ethylene-co-vinyl acetate) (EVA). As preliminary research, the ‘in vivo’ biocompatibility of PDMS and EVA in the intra-peritoneal cavity was confirmed by implantation of PDMS and EVA rod-shaped implants in rats. The kinetic of release was determined ‘in vitro’ and confirmed ‘in vivo’. Besides, the efficiency of the implants was improved by the addition of a polymer membrane, which allowed a controlled release of anastrozole over a period of 400 days. [less ▲]

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See detailStudy of photosynthesis of Chlamydomonas reinhardtii under High and Low CO2 conditions.
de Marchin, Thomas ULg; Ghysels, Bart ULg; Franck, Fabrice ULg

Poster (2013, August 12)

In photoautotophically air-grown microalgae, CO2 availability is usually limited. The unicellular green algae Chlamydomonas reinhardtii can adapt to low CO2 concentration with the inorganic carbon ... [more ▼]

In photoautotophically air-grown microalgae, CO2 availability is usually limited. The unicellular green algae Chlamydomonas reinhardtii can adapt to low CO2 concentration with the inorganic carbon concentration mechanism (CCM). This has been extensively studied in the past but functional adaptation of the photosynthetic apparatus has been less studied. Photosynthetic organisms can cope with CO2 limitation by dissipating excess absorbed energy with the help of different energy dissipating mechanisms like energy-dependent non-photochemical quenching (NPQ). In Chlamydomonas reinhardtii, this process only seems to develop to high levels in extreme conditions combining high light and strong CO2 limitation. Under moderate CO2 limitation, absence of important energy-dependent NPQ suggests the development of another energy dissipating mechanism. We compared the growth and functional adaptations of the photosynthetic apparatus of the wild-type strain 1690 grown in photobioreactor under low and high CO2 bubbling, 0.039% and 10%, respectively. Under low CO2, where growth was 2 to 4 times slower than under high CO2, the non-linear relationship between electron transport rate (derived from PAM fluorescence measurements) and gross oxygen evolution rate suggested that a significant portion of the electron flux is directed to oxygen at light intensities approaching photosynthetic saturation (either at PSI or at PTOX). The use of the mutant strain PTOX2 indicated that O2 reduction occurs mainly at PSI and not at PTOX. Low temperature fluorescence emission spectra indicated no significant difference in excitation energy distribution between PSI and PSII. Western blot analysis showed no major differences in abundance of Rubisco or of photosystem subunits between the two conditions. In contrast, cytochrome f abundance was lower in high CO2 condition. Although energy-dependent NPQ remained weak, low CO2 cells were characterized by a higher xanthophyll deepoxydation index which usually indicates more dissipation as heat, as also suggested by increased Lhcsr3 expression. Despite a higher ATP requirement of the CCM mechanism in low CO2 condition, only minor difference in cyclic electron transport could be found if compared to high CO2 condition (as determined by P700 spectroscopic measurements). In Chlamydomonas, conflicting views were expressed in earlier studies on the amplitude and role of Mehler-type O2-uptake at steady state. Our analysis of oxygen evolution, electron transport and NPQ after growth under different combinations of light intensities and CO2 supply rates allows us to define Mehler-type alternative electron transport as an important and flexible response to photosynthetic electron transport saturation in Chlamydomonas. [less ▲]

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See detailInteractions with musical long-term memory are a critical component of musical working memory
Gorin, Simon ULg; Majerus, Steve ULg

Poster (2013, August 10)

The nature and mechanisms of working memory (WM) for musical information remain poorly understood. The aim of this study is to show that musical WM strongly depends upon long-term memory (LTM) mechanisms ... [more ▼]

The nature and mechanisms of working memory (WM) for musical information remain poorly understood. The aim of this study is to show that musical WM strongly depends upon long-term memory (LTM) mechanisms and requires access to the long-term musical knowledge base. Two groups of participants (musicians and non-musicians) participated first in an implicit learning task during which they heard for about 30 minutes a continuous sequence of tones governed by a new musical grammar. Then, they performed an immediate serial recall task of musical sequences of increasing length; half of the sequences were constructed in accordance to the rules of the new grammar presented during the implicit learning task. Participants have to reproduce the sequences by humming and their performances were calculated on the basis of the deviation between their production and the stimulus needed to be reproduced. The results showed a significant advantage for the lists governed by the grammar previously learned. Overall, this study shows that performance on a musical WM task is enhanced by musical knowledge stored in LTM. This study is the first to demonstrate the dependency of musical WM on musical LTM knowledge, implying that existing models of musical WM need to be extended to account for this WM-LTM interaction. [less ▲]

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See detailProfitability of using warning system for foliar disease of wheat in the Grand-Duchy of Luxembourg.
El Jarroudi, Moussa ULg; Kouadio, Louis; Beyer, Marco et al

Poster (2013, August 10)

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See detailFacile Encapsulation of Sn nanocrystals in GeS2 Matrix for High-Performance Lithium-ion Battery Anodes
Singh, Ajay; Krins, Natacha ULg; Milliron, Delia J.

Poster (2013, August 05)

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See detailWarming promotes cheatgrass invasion in mixed-grass prairie
Blumenthal, Dana; Kray, Julie; Morgan, Jack et al

Poster (2013, August 04)

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See detailSpatial analysis of bark-stripping damage by red deer in irregular hardwood forest
Lehaire, François ULg; Mercier, Grégoire; Lejeune, Philippe ULg

Poster (2013, August)

Over the past years, the population size of red deer (Cervus elaphus L.) is following an upward trend in Wallonia. This has led to an increased pressure on vegetation as well as to forest damages of ... [more ▼]

Over the past years, the population size of red deer (Cervus elaphus L.) is following an upward trend in Wallonia. This has led to an increased pressure on vegetation as well as to forest damages of greater frequency and intensity. Among these damages, bark-stripping can be especially harmful to the timber quality due to the development of rot. The impact of these populations may thus lead to a reduction in forest productivity and to economic losses. Nevertheless, these damages doesn’t depend only on the density of red deer but also on the environmental characteristics of their habitats. The main objective of this study is to perform a spatial analysis of the occurrence of bark stripping in order to identify the environmental factor that affect the occurrence of the damages in hardwood forests. The bark-stripping damages in coniferous stands are well-known due to the fact that they are relatively frequent. Such information is however unavailable for hardwood stands. The study site (6500 hectares) is located in the Ardennes in Southern Belgium (Wallonia) and is mainly composed of beech stands. We used data of inventory campaigns that were carried out for management purposes. In each sampling unit (total of 321 units), we measured different stand characteristics and, during a second phase, the bark-stripping damages. These latter measurements concerned the presence or absence of bark-stripping on each recorded stem and damage age (presence or not of a healing roll). Only trees with dbh greater than 5 cm were taken into account. All the data were collected in hardwood stands. To perform the spatial analysis, a set of environmental variables, including landscape (distances to the natural or artificial feeding points, to watering-place, to the different types of road and to the forest paths, to refuge areas for deer and to forest edges, etc.) and tree dimension variables, have been collected. The estimated red deer density was also took into account. All these variables have been included in a fixed linear model using stepwise regression. An angular transformation was applied in order to guarantee appropriate conditions of application of the linear regressions. Over one hundred variables were tested but only six of them have a significant impact on the bark-stripping rate. This model explains only 10,2% of the bark-stripping rate. Tree dimension variables explain most of the bark-stripping rate. Thus, forest structure has an important impact on the bark-stripping probability. The roads and the human activities in general can have an impact on the bark-stripping rate. These activities can disturb the red deer feeding periods during the day and lead to important bark-stripping damages. We expected to observe other variables contributing to the model such as red deer density and altitude. The absence of effect of altitude can be explained by the fact that bark-stripping of beech trees occurs mainly in summer. The scale of the study can explain the absence of red deer density. [less ▲]

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