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See detailRegioselective labeling of Nanofitin by using a phosphorylated peptide tag
Goux, Marine ULg

Poster (2014, October)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 or gallium-68. Despite their important role in ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as zirconium-89 or gallium-68. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the slow clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag. A specific phosphorylation step, with the alpha subunit of the casein kinase II, generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray [1]. We are now adapting the use of this labeling tag to the stereoselective chelation of radionuclides for PET imaging. As described previously, functionalizing the tag with a phosphate nanocluster is a key step to provide the capacity to specifically chelate metal ions such as zirconium or gallium. In order to optimize the sequence of the phosphorylatable tag, we have used mimetic peptides with different phosphorylation states (from 0 to 4 phospho-serine). We have shown the complexation between the phosphorylatable tag and a lanthanide: terbium(III). With a competition study between terbium(III) and zirconium(IV), we showed that the peptide tag preferably chelate zirconium with an affinity in the micromolar range. Considering this tag has been created to specifically anchoring protein on zirconium phosphonate-based microarray, it is possible that the sequence is not optimized for our application. In order to increase the affinity from micromolar to nanomolar, we are currently working on a sequence derived from calcium-binding proteins to chelate specifically lanthanides[2]. As described by Pardoux et al.[3], our strategy is to functionalize this sequence with a phosphate nanocluster able to chelate zirconium or gallium. Our first results shown that a mono-phosphorylatable tag phosphorylated in vitro is able to chelate terbium(III) with a lower affinity than the wild type. Considering that terbium(III) is bigger than gallium(III) or zirconium(IV), we can suppose that the cage obtained is too small but suitable for other ions. In order to validate our hypothesis, we have planned to radiolabel those tags and determine their affinity for gallium(III). [1] M. Cinier, M. Petit, F. Pecorari, D. R. Talham, B. Bujoli, and C. Tellier, “Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays.,” J. Biol. Inorg. Chem., vol. 17, no. 3, pp. 399–407, Mar. 2012. [2] L. J. Martin, “Development of Lanthanide-Binding Tags (LBTs) as powerful and versatile peptides for use in studies of proteins and protein interactions,” 2008. [3] R. Pardoux, S. Sauge-merle, D. Lemaire, P. Delangle, L. Guilloreau, J. Adriano, and C. Berthomieu, “Modulating Uranium Binding Affinity in Engineered Calmodulin EF-Hand Peptides : Effect of Phosphorylation,” PLoS One, vol. 7, no. 8, 2012. [less ▲]

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See detailA propos de deux cas de manipulation de patients dans le cadre de recherches cliniques
Jamoulle, Marc ULg

Poster (2014, October)

L’exercice de la prévention quaternaire, soit veiller à ne pas nuire au patient, est mis ici à contribution dans deux cas de recherches cliniques. Deux patients ont vécu dans le même service de ... [more ▼]

L’exercice de la prévention quaternaire, soit veiller à ne pas nuire au patient, est mis ici à contribution dans deux cas de recherches cliniques. Deux patients ont vécu dans le même service de cardiologie une tentative de manipulation lors de recherches, l’une pharmacologique, l’autre de traitement invasif. Le questionnement des patients a été discutés au cours de la consultation de médecine générale. Les deux patients ont chacun relu et compris la portée de leur témoignage publié ci-dessous et marquent leur accord formel à leur publication. [less ▲]

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See detailDesigning large-scale CO2 capture units with assessment of solvent degradation
Léonard, Grégoire ULg; Crosset, Cyril; Dumont, Marie-Noëlle ULg et al

Poster (2014, October)

Solvent degradation is a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. In the present work, we refine a ... [more ▼]

Solvent degradation is a major drawback for the large-scale implementation of post-combustion CO2 capture due to amine consumption and emission of degradation products. In the present work, we refine a previous kinetic model for describing solvent oxidative and thermal degradation based on experimental results. The CO2 capture process is then modeled in Aspen Plus with assessment of solvent degradation. As a result, this work provides a useful tool for the identification of optimal operating conditions that minimize both the energy and environmental impacts of the process. [less ▲]

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See detailTSC2 and 14-3-3 proteins down-regulate a RIP3 dependent PDT-induced Necroptosis in Glioblastoma
Fettweis, Grégory ULg; Coupienne, Isabelle; Fillet, Marianne ULg et al

Poster (2014, October)

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See detailExperimental in vivo infection of pigs by a Belgian wild boar hepatitis E virus strain
Thiry, Damien ULg; Rose, Nicolas; Paboeuf, Frédéric et al

Poster (2014, October)

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See detailLe développement et la validation du processus de raisonnement clinique
Collard, Anne ULg; Bourguignon, Jean-Pierre ULg

Poster (2014, October)

Contexte : La Faculté de Médecine de l’Université de Liège propose dès la 2ème année de son cursus un enseignement de type PBL. Nous avons étudié le développement des capacités de raisonnement en lien ... [more ▼]

Contexte : La Faculté de Médecine de l’Université de Liège propose dès la 2ème année de son cursus un enseignement de type PBL. Nous avons étudié le développement des capacités de raisonnement en lien avec la base de connaissances et son utilisation. Méthodes : Le développement des capacités de raisonnement est étudié de façon transversale par l’utilisation d’un TCS ainsi que longitudinalement exploitant les résultats des évaluations certificatives de raisonnement. Les niveaux de certitude aux réponses correctes et incorrectes aux tests de connaissances sont utilisés pour calculer un indice d’auto-évaluation du champ des connaissances sous-jacentes au raisonnement. Par ailleurs, l’effet d’une consigne de validation du processus de raisonnement en cours de séance PBL a également été étudié. Résultats : La capacité de raisonnement est manifeste dès la 2ème année et augmente avec l’expérience. Elle est corrélée à la capacité d’auto-évaluation du champ des connaissances. De plus, la validation du processus de raisonnement peut être renforcée par une consigne explicite et favorise ainsi l’apprentissage du raisonnement. Conclusion : Ces résultats ont abouti à l’élaboration d’un modèle permettant de mettre en perspective les différents facteurs impliqués dans le processus d’apprentissage du raisonnement en lien avec la base de connaissances et son utilisation. [less ▲]

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See detailAdherence to Continuous Positive Airway Pressure (CPAP) Therapy
Deflandre, Eric ULg; Degey, Stéphanie; BONHOMME, Vincent ULg et al

Poster (2014, October)

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See detailThe cellobiose-sensor CebR is the gatekeeper of Streptomyces scabies pathogenicity
Jourdan, Samuel ULg; Francis, Isolde; Loria, Rosemary et al

Poster (2014, October)

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See detailTemplating calcium carbonate drug delivery carriers based on polyphosphoester copolymers
Ergül, Zeynep ULg; Debuigne, Antoine ULg; Calvignac, Brice et al

Poster (2014, October)

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See detailHow snow affects air-sea ice CO2 fluxes
Delille, Bruno ULg; Kotovitch, Marie ULg; Van Der Linden, Fanny ULg et al

Poster (2014, October)

Sea ice is a significant contributor to the sink of atmospheric CO2 by polar oceans. Physical and biogeochemical sea ice processes affect partial pressure of CO2 within sea ice, that in turn controls the ... [more ▼]

Sea ice is a significant contributor to the sink of atmospheric CO2 by polar oceans. Physical and biogeochemical sea ice processes affect partial pressure of CO2 within sea ice, that in turn controls the way and magnitude of air-sea ice CO2 fluxes. Snow cover appears to affect the magnitude of the fluxes. In order to understand the role of snow, we compared chamber and micrometeorological measurements of air-ice CO2 fluxes over snow covered and uncovered sea ice (land fast and pack ice) in both arctic and antarctic. We observed significant differences between fluxes over uncovered and covered sea ice. In addition chamber and micrometeorological measurement show different patterns that are partially due to snow cover. By gathering these observations, we observed at least three effects of snow on air-ice CO2 fluxes. Snow appears to (i) act as transient CO2 reservoir (ii) affect thermal properties of the ice surface (iii) control gas transfer depending on snow structure (superimposed ice, slush). [less ▲]

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See detailEmotional distress and family factors as predictors of quality of life in Children with Cystic Fibrosis
Toucheque, Malorie ULg

Poster (2014, October)

Background. Due to recently developed therapies, patients’ life expectancy with CF today is in the early 40s. As a result, medical providers in CF care centers seek not only to improve patients’ health ... [more ▼]

Background. Due to recently developed therapies, patients’ life expectancy with CF today is in the early 40s. As a result, medical providers in CF care centers seek not only to improve patients’ health but also to enhance their quality of life (QOL). QOL as a concept for children, in particular, is fairly new. Accordingly, QOL of school-aged children with CF is relatively under-investigated. In recent years, the role of family contextual variables has received increased attention as predictors of child adjustment to illness. Evidence exists that parenting and parent-child relationship factors are related to children’s emotional development and thus may be life-course determinants in their health. To date, no study has quantitatively investigated parental factors, particularly with respect to paternal variables, associated with the QOL of children with CF. Objective. The study aimed to investigate the relation of parenting stress, family functioning, and fathers’ involvement in the disease’s management to emotional distress and QOL in children with CF. Study design. Using a cross-sectional design, this study recruited families of children with CF from four different CF Centers. Data collection is on going and a full sample of 40 families is expected for this presentation. To date, the study protocol was completed by 18 families. Inclusion criteria for children include: (a) a diagnosis of CF for at least one year; and (b) age 8 to 12 years. Also, both parents must be willing to participate to be included in our sample. Children complete the State-Trait Anxiety Inventory for Children (STAIC), Children’s Depression Inventory (CDI-2), and Quality of Life Systemic Inventory for Children (QLSI-C). Parents complete the Parenting Stress Index – Short Form (PSI-4-SF), Family Environment Scale (FES), and Dad’s Active Disease Support (DADS) (i.e., a measure of paternal involvement in illness management). Results. With the full sample, we will run multiple regression analyses with STAIC, CDI, PSI-4-SF, FES and DADS as predictors of children’s QOL scores and run moderation and mediation analyses to examine the indirect effect of mothers and fathers’ variables on the child’s QOL. With the sample to date, QOL in children is significantly associated with state anxiety (r=.62; p=.007), trait anxiety (r=.76; p<.001), and depression (r=.77; p=.001). We also found a direct link between the mothers’ parenting stress levels and children’s QOL (r=.51; p=.031), anxiety (r=.49; p=.045), and depression (r=.47; p=.054). Fathers’ parenting stress levels (r=.54; p=.026) and mothers’ evaluation of fathers’ involvement (r=.47; p=.047) each are significantly associated with the mothers’ parenting stress. These results may be suggestive of a potential mediator effect. Conclusion. Information about fathers is underrepresented in pediatric psychology research. However, interest in impact of parental adaptation on children’s adjustment to chronic disease is on the increase. The results of this study are expected to contribute significantly to the understanding of how both parents play a role in the QOL and emotional adjustment of school-aged children with CF. [less ▲]

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See detailHarmonia axyridis population study in agroecosystems
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Durieux, Delphine ULg et al

Poster (2014, October)

The Multicoloured Asian ladybird, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is one of these species which was intentionally introduced for biological control and has spread from its native ... [more ▼]

The Multicoloured Asian ladybird, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is one of these species which was intentionally introduced for biological control and has spread from its native range in Central and Eastern Asia to large parts of North and South America, Europe, and Africa. The decline of native species is linked to the spread and the aggressive behaviour of H. axyridis. From 2009, we evaluated the population densities of aphids predator and predator diversities, with a special focus on H. axyridis in agroecosystems of Wallonia (South of Belgium). First, field crops preferences of H. axyridis were determined. Later, changes linked to aphid’s predator populations over time were evaluated. Finally, H. axyridis population changes between two crop farming methods were also evaluated. Samplings performed in agroecosystems highlighted that the community of aphid's predator is composed of few dominant species: three coccinellids (Coccinella septempunctata L., P. quatuordecimpunctata L., and H. axyridis), one syrphid (Episyrphus balteatus De Geer), and one chrysopid (Chrysoperla carnea Stephens). We demonstrated that H. axyridis do not invade all crops at the same rate, maize and broad bean being more infested than wheat and potato at both larval and adult stages. Moreover, H. axyridis populations in maize have strongly increased from 2009 to 2011. Finally, we showed that organic farming do not contribute to increase the abundance of H. axyridis but leads to increase the total abundance of aphid’s natural enemies. [less ▲]

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See detailStudy of sex ratio and morphotypes of the Multicoloured Asian Ladybird, Harmonia axyridis Pallas in Belgian maize
Vandereycken, Axel ULg; Fassotte, Bérénice ULg; Barsics, Fanny ULg et al

Poster (2014, October)

Several insect species are known to have different morphotypes, an adaptation to heterogeneities in changing environments. This phenotypic plasticity could be a factor used by the Multicoloured Asian ... [more ▼]

Several insect species are known to have different morphotypes, an adaptation to heterogeneities in changing environments. This phenotypic plasticity could be a factor used by the Multicoloured Asian Ladybird, which is considered as an invasive species in Europe since its introduction in the 80’s. Harmonia axyridis has four major morphotypes: 2 melanic forms, conspicua and spectabilis; and 2 non-melanic, succinea and axyridis. In Belgium, only the three first are observed. Literature reports variations in morphotype frequencies, across native and introduced strains. These variations seem to be linked to climate (geographical and seasonal variation), with non-melanic forms being the most abundant in hot and arid climate. This study focused on the variation of morphotype abundance in H. axyridis according to season, field and gender. Adult ladybirds were caught from mid-July to mid-October 2012 into 5 maize fields. The proportions of observed morphotypes were compared according to the sampling season. The abundance of morphotypes was not significantly different through the fields; the observed ratios were 81.6%, 15% and 3.4% for succinea, spectabilis and conspicua respectively. Succinea was the most observed morphotype (χ2=755.8; P<0.001). The total number of collected males and females was the same with ratios of 48.2% and 51.8%: (χ2=0.93; P=0.33). The sex ratio was also not significantly different for each morphotype. There was no evolution of melanic and non-melanic form ratio according to the season, with 25.6% of melanic form in summer and 20.6% in autumn (χ2=1.22; P=0.27). Our results seem to show that morphotype adaptation according to the climate is not observed for Belgian ladybirds. [less ▲]

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See detailSinuso-nasal adenocarcinoma in a roe deer (Capreolus capreolus)
Volpe, Rosario ULg; Cassart, Dominique ULg; Neukermans, Axel et al

Poster (2014, October)

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See detailAdherence to Continuous Positive Airway Pressure (CPAP) Therapy
Deflandre, Eric ULg; Degey, Stéphanie; BONHOMME, Vincent ULg et al

Poster (2014, October)

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See detailCharacterization of stream - aquifer interaction in carbonate rocks
Briers, Pierre ULg; Sohier, Catherine ULg; Schmit, Flore et al

Poster (2014, September 30)

Groundwater - surface water interactions play a fundamental role in terms of quantity and quality of water and in terms of ecological quality of rivers. Despite many research efforts and the necessity to ... [more ▼]

Groundwater - surface water interactions play a fundamental role in terms of quantity and quality of water and in terms of ecological quality of rivers. Despite many research efforts and the necessity to better understand such interactions in order to reach effective management of water resources, stream-aquifer exchanges remain poorly understood, in particular in fractured carbonate environments. [less ▲]

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See detailFrench good practice guidelines for the management of the low back pain risk among workers exposed to manual handling
Petit, Audrey; Mairiaux, Philippe ULg; FASSIER, Jean-Baptiste et al

Poster (2014, September 29)

Background Manual handling of loads (MHL) is a widespread practice in the workplace and is the leading cause of work accidents. In particular, MHL is associated with a risk of low back injuries which can ... [more ▼]

Background Manual handling of loads (MHL) is a widespread practice in the workplace and is the leading cause of work accidents. In particular, MHL is associated with a risk of low back injuries which can compromise staying at work and worker's career in the case of recurrence or chronicity. This communication aims to set forth the French occupational Guidelines for the management of the low back pain (LBP) risk among workers exposed to MHL, published in October 2013. Methods These Good Practice Guidelines were written according to the Clinical Practice Guidelines method proposed by the French National Authority for Health. They emanated from a synthesis of the literature by a multidisciplinary working party of 24 experts and were peer reviewed by a committee of 50 experts. These Guidelines are designed to define the components of an appropriate surveillance program for all workers exposed to MHL activities at the workplace and especially suffering from LBP and still at work, or suffering LBP and on sick leave. Results Because of the potential impact on workers’ fears and beliefs, the information provided by the various actors must be consistent, or even reassuring in relation to the prognosis of LBP. Among LBP workers, it is recommended to look for signs of severity or an underlying disease at the acute, subacute and chronic stages of LBP; to encourage continuation or resumption of physical activity; situate the current episode of LBP in the worker’s work history (recent changes of working conditions) and evaluate the impact of LBP on the worker’s job. In the case of persistent or recurrent LBP, it is recommended to evaluate biopsychosocial and socioeconomic risk factors likely to influence chronicity, prolonged incapacity and delay return to work. If workers are on prolonged and/or repeated sick leave for LBP, a pre-return-to-work visit is recommended to evaluate the pain and functional disability and their repercussions, as well as the main factors of prolonged work incapacity; encourage and help the worker to develop a return to work dynamic; identify the main difficulties related to work and possible job adjustments and evaluate the need to initiate a job staying at work approach. Conclusions Primary, secondary and tertiary prevention are necessary for the management of low back pain at work. [less ▲]

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See detailFITNESS EVALUATION OF A RECOMBINANT MURINE NOROVIRUS DURING SERIAL PASSAGES IN CELL CULTURE
Oliveira-Filho, Edmilson; Di Felice, Elisabetta; Toffoli, Barbara et al

Poster (2014, September 28)

Noroviruses are single stranded positive sense RNA viruses which can infect human and different animal species. Human norovirus (NoV) infections are among the most important causes of gastroenteritis in ... [more ▼]

Noroviruses are single stranded positive sense RNA viruses which can infect human and different animal species. Human norovirus (NoV) infections are among the most important causes of gastroenteritis in both children and adults. Infections often occur as outbreaks which may be foodborne. Due to the lack of an efficient cell culture system as well as a workable animal model, many aspects of the NoV infection in human are still poorly understood. The murine norovirus (MuNoV) grows easily in cell culture in contrast to the Human NoV, and constitutes an excellent animal model. Recombination can dramatically change virulence properties of the viruses and has been evidenced in silico for different human NoV strains isolated from clinical cases. Recently, after in vitro coinfection of RAW264.7 cells with parental MuNoV strains CW1 and Wu20, we obtained a recombinant Wu20/CW1 strain. This recombinant strain showed reduced plaque size compared to the parental strains. The aim of the study was to observe and molecularly characterize the natural genetic evolution of the recombinant MuNoV strain across in vitro replications. Viral fitness is a complex concept. Here we defined this fitness as the ability of a viral population to adapt to the cell culture system. Thus, the recombinant strain was serially replicated in vitro in RAW264.7 cells (up to 14 passages). Viral plaque sizes of early and late progenies were compared with the Image J software. A significant difference was shown between them with the Mann and Whitney non parametric statistical test. Afterwards, viruses from different cell passages were cloned and sequenced. The average plaque size increased from the earlier to the later progenies (from 0.1 mm2 to around 0.5 mm2). Molecular investigations are currently performed in order to specify in which genetic region mutations occur and whether or not this could explain fitness modifications during in vitro evolution. In addition, two other parameters of in vitro virulence modification will be investigated: (i) virus production and (ii) one step growth kinetics. The data should provide interesting information about genetic evolution in the genus Norovirus, especially regarding recombination events and explain how a recombinant strain, first disadvantaged compared to its parental strains, could regain fitness by genetic evolution. [less ▲]

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See detailLe secret des crèmes foisonnées
Petrut, Raul Flaviu ULg; Danthine, Sabine ULg; Blecker, Christophe ULg

Poster (2014, September 26)

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See detailHDAC5 Depletion in Cancer Cells Induces an Oxidative Stress and Leads to a Metabolic Reprogramming toward Glucose and Glutamine Metabolism
Hendrick, Elodie ULg; Peixoto, Paul ULg; Polese, Catherine ULg et al

Poster (2014, September 25)

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum ... [more ▼]

Histone deacetylases (HDAC) is a family of eighteen enzymes, which modulates the acetylation level of histones and non-histone proteins to regulate gene expression and chromatin structure. Broad-spectrum inhibitors of these enzymes such as SAHA can inhibit tumor growth both in vitro and in vivo and are currently used as anti-cancer agents in clinic. For many years, we are investigating the specific role of individual HDAC members in cancer biology and we have recently demonstrated that specific depletion of HDAC5 using siRNA technology reduced cancer cells proliferation and survival1 The goal of this study is to further understand the molecular mechanisms of action of HDAC5 in cancer cells. Screening transcriptomic study demonstrated that HDAC5 depletion induces a down-regulation of subunits of the complex I of the mitochondrial respiratory chain (NDUFB5-NDUFA3) as well as anti-oxydant proteins (Ferritin, Metalothionein,¿) through modulation of mRNA stability. Therefore, HDAC5 depletion causes a significant increase of ROS production inducing both apoptosis and mechanisms of mitochondria quality control (mitophagy and mitobiogenesis). This HDAC5 depletion-induced mitochondrial dysfunction provokes metabolic adaptation associated with increased importance of glycolysis and glucose. Indeed, interference with glucose supply in HDAC5-depleted cancer cells significantly increases apoptotic cell death suggesting that glucose deprivation might be combined to HDAC5 inhibition as a therapeutic strategy to kill cancer cells. Our study demonstrated for the first time that specific HDAC5 inhibition induces metabolic reprogramming and provides insight into a valuable experimental strategy for manipulation of specific HDAC5 inhibition and glucose metabolism in therapy against cancer. 1.Peixoto, P. et al. HDAC5 is required for maintenance of pericentric heterochromatin, and controls cell-cycle progression and survival of human cancer cells. Cell death and differentiation, 2012; 1-14. Presenting author e-mail: elodie.hendrick@student.ulg.ac.be [less ▲]

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See detailCarbon balance of a grazed pasture and its response to grazing management
Jerome, Elisabeth ULg; Gourlez de la Motte, Louis ULg; Beckers, Yves et al

Poster (2014, September 24)

The C balance of a grazed pasture situated in Condroz (Wallonia) and its dependence on climatic conditions and grazing management were investigated on the basis of eddy covariance measurements and ... [more ▼]

The C balance of a grazed pasture situated in Condroz (Wallonia) and its dependence on climatic conditions and grazing management were investigated on the basis of eddy covariance measurements and horizontal C flow estimates. In average on three years, NEE was +43±24 gCm-2yr-1 and NBP was +7±26 gCm-2yr-1, suggesting that that the site is C neutral. Management by the farmer (organic fertilization), but also climate conditions influencing management (feed supplements), were the main factors impacting the C balance inter-annual variability. At a daily and seasonal scale, grazing impact on CO2 fluxes did not appear explicitly, being blurred by flux response to climate drivers. It was highlighted by specific investigations. The indirect grazing impact (photosynthesizing biomass consumption, excretions, soil compaction) was deduced from an analysis of the flux to PPFD response evolution during grazing period; the direct impact (livestock respiration) was investigated through confinement experiments. Result showed that saturation GPP changes were negatively correlated to grazing intensity (product of the stocking rate and grazing duration). On the contrary, no significant change in TER was observed. The direct impact of grazing due to cattle respiration was estimated to 2.59±0.58 kgCLU-1day-1, i.e. 8% of the TER. [less ▲]

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See detailImportance des modèles de distribution de niche potentielle dans la gestion des espèces tropicales exploitées: cas des taxons du genre Guibourtia Benn
Tosso, Dji-ndé Félicien ULg; Daïnou, Kasso ULg; Hambuckers, Alain ULg et al

Poster (2014, September 24)

De la famille des (Fabaceae / Caesalpinioideae) et composé de 13 espèces en Afrique, le genre Guibourtia comporte des taxons à forte valeur culturelle et à forte valeur commerciale. Aujourd'hui, la ... [more ▼]

De la famille des (Fabaceae / Caesalpinioideae) et composé de 13 espèces en Afrique, le genre Guibourtia comporte des taxons à forte valeur culturelle et à forte valeur commerciale. Aujourd'hui, la pression de l'exploitation combinée aux faibles densités de ce genre, fait a priori peser d’importantes menaces sur certaines de ses populations. Un projet de recherche a donc été initié afin de mieux comprendre la structure et la diversité génétique des populations de Guibourtia, en lien avec l'exploitation forestière et les patrons de reproduction spécifiques. Un premier volet de la recherche a consisté à identifier les déterminants climatiques expliquant la distribution des espèces. Nous avons combinée des modèles statiques (Maxent et régression logistique) avec des données du modèle climatique global CNRM CM5, et sur la base de l'occurrence de ces taxons entre 1950 et 2000. Il en ressort que les espèces du genre Guibourtia sont sensibles aux facteurs précipitation (69,2 %) et amplitude thermique (74,3 %). Dans un second temps, il sera utilisé les modèles climatiques des ères géologiques passées afin d'inférer la distribution de l'espèce au cours du Quaternaire, et de faire le lien avec des analyses phylogénétiques et phylogéographiques. Il sera également possible d'évaluer la distribution future de l'espèce tenant compte des modèles d'évolution du climat. Enfin, le projet de recherche s'attèlera particulièrement aux relations phylogénétiques entre espèces morphologiquement similaires en sympatrie ou parapatrie, en caractérisant en détail les flux de gènes entre individus de ces taxons proches, ainsi que leur degré de similarité physiologique. Les résultats de l’étude in fine contribueront à proposer des stratégies de conservation et de gestion durable dans le contexte de l’exploitation forestière d’Afrique centrale et du changement climatique en cours. [less ▲]

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See detailICOS IN BELGIUM: WALLONIA
De Ligne, Anne ULg

Poster (2014, September 23)

Présentation ICOS Wallonia

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See detailIRGA GAS SAMPLING SYSTEM DIMENSIONING: LABORATORY AND FIELD EXPERIMENTS
De Ligne, Anne ULg; Joly, Lilian; Cousin, Julien et al

Poster (2014, September 23)

The gas sampling system (GSS), which carries air from the sampling point to the IRGA, is an essential component of the eddy covariance system. It has to meet several constraints, among which minimizing ... [more ▼]

The gas sampling system (GSS), which carries air from the sampling point to the IRGA, is an essential component of the eddy covariance system. It has to meet several constraints, among which minimizing high frequency attenuation of concentration measurement and keeping pressure drop in the measurement cell in an acceptable range. Rain cup, filters, tubes and pumps are key elements of this system and need proper dimensioning. The elaboration of the ICOS protocol for IRGA required such dimensioning and optimization. Laboratory and field measurements were carried out with this aim. In the laboratory, a dynamic calibration bench was developed to investigate experimentally the pressure drop and the concentration fluctuation attenuation caused by different filters. In the field, three LI-7200 equipped with different GSS were installed and run at the Dorinne Terrestrial Observatory (ICOS-Belgium). Main experiment conclusions were that: • The shape and size of the rain cup has a critical impact on cut off frequencies • The filter porosity and size has a critical impact on pressure drop • Filter heating is necessary in order to avoid condensation and filter blocking These experiments led to the definition of the GSS functioning range that is finally proposed in the ICOS IRGA protocol. [less ▲]

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See detailSeasonal and inter-annual variability of photosynthetic capacity in a temperate forest
Hurdebise, Quentin ULg; Vincke, Caroline; Soubie, Rémy et al

Poster (2014, September 23)

In temperate forests, the relation between vegetation phenology and carbon sequestration variability remains to be explored. To study phenology impact on CO2 fluxes, two photosynthetic capacity indicators ... [more ▼]

In temperate forests, the relation between vegetation phenology and carbon sequestration variability remains to be explored. To study phenology impact on CO2 fluxes, two photosynthetic capacity indicators were used : NEE at light saturation (NEEsat) and green proportion in canopy pictures (gcc). Correlations between these indicators were investigated at seasonal and inter-annual scale. [less ▲]

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See detailAre agricultural ecosystems important BVOC « exchangers »? Evidences from 2 measurement years on croplands at Lonzée Terrestrial Observatory (Belgium)
Bachy, Aurélie ULg; Aubinet, Marc ULg; Schoon, Niels et al

Poster (2014, September 23)

For the last decades, agricultural ecosystems have been a key biome for diverse socio-economical, environmental and climatic issues. And one of these climatic issues is just BVOC (Biogenic Volatile ... [more ▼]

For the last decades, agricultural ecosystems have been a key biome for diverse socio-economical, environmental and climatic issues. And one of these climatic issues is just BVOC (Biogenic Volatile Organic Compounds) emission from terrestrial ecosystems. Indeed, those compounds which are mostly emitted by plants play a great role in the atmospheric chemistry, thereby influencing the Earth surface radiative budget and the tropospheric air quality. However, so far, very few is known about BVOC exchange by crops, implying that huge uncertainties remain about qualifying, quantifying and determining sources/sinks and driving mechanisms of BVOC exchanges between croplands ecosystems and the atmosphere. We present here the first long term BVOC fluxes measurement study conducted on maize (2012) and winter wheat (2013), respectively the second and first most important worldwide crops (FAOSTAT). BVOC exchange was measured using the disjunct by mass scanning eddy covariance technique (+ PTR-MS, Ionicon) at the Lonzée Terrestrial Observatory (ICOS site) in Belgium. Main results are: (i) crops emit mainly methanol; (ii) BVOC emission from studied crops is lower than in literature, suggesting that agricultural ecosystems are poor BVOC exchangers; (iii) soil is a significant BVOC source. [less ▲]

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See detailA heat and dye tracer test for characterizing and modelling heat transfer in an alluvial aquifer
Klepikova, Maria ULg; Wildemeersch, Samuel; Jamin, Pierre ULg et al

Poster (2014, September 22)

Using heat as an active tracer is a topic of increasing interest with regards to characterizing shallow aquifers for ATES (Aquifer Thermal Energy Storage) systems. In this study, we investigate the ... [more ▼]

Using heat as an active tracer is a topic of increasing interest with regards to characterizing shallow aquifers for ATES (Aquifer Thermal Energy Storage) systems. In this study, we investigate the potential interest of coupling simultaneous heat and dye tracer injection tests for characterization of an alluvial aquifer. The study site is located near Liege in the alluvial aquifer of the Meuse River, Belgium. The tracing experiment consisted in simultaneously injecting heated water and a dye tracer in a piezometer and monitoring the evolution of groundwater temperature and tracer concentration in the recovery well and in nine monitoring wells located according to three transects with regards to the main groundwater flow direction. The breakthrough curves measured in the recovery well showed that heat transfer in the alluvial aquifer is slower and more dispersive than solute transport. Recovery is very low for heat while in the same time it is measured as relatively high for the solute tracer. This shows how heat diffusion is larger than molecular diffusion, implying that exchange between groundwater and the porous medium matrix is far more significant for heat than for solute tracers. In a first step, temperature and concentrations in the recovery well are used for estimating the specific heat capacity with an energy balance calculation and the estimated value is found to be consistent with those found in the literature. Then, the measured temperature breakthrough curves in the piezometers are used for constraining the heat transport model. They are highly contrasted with what would be expected in an ideal layered aquifer. They reveal strongly unequal lateral and vertical components of the transport mechanisms. A preliminary interpretation of these temperature breakthrough curves is provided with first results from the model. Then it will allow for estimating the entire set of heat transfer parameters and their spatial distribution by inverse modelling. The developed concepts and tests may lead to real projects of various extents that can be now optimized by the use of a rigorous and efficient methodology at the field scale. [less ▲]

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See detailAmphiphilic copolymers containing polyphosphates for drug delivery applications
Vanslambrouck, Stéphanie ULg; Clément, Benoit; Riva, Raphaël ULg et al

Poster (2014, September 19)

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See detailBehavior of Listeria monocytogenes in sheep raw milk cheeses: a study at different stages of production and shelf-life"
N'Guessan, Elise; Amara, Nassima; Godrie, Thérèse ULg et al

Poster (2014, September 18)

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See detailAutomatic artifact detection for whole-night polysomnographic sleep recordings
Coppieters't Wallant, Dorothée ULg; Chellappa, Sarah Laxhmi ULg; Gaggioni, Giulia ULg et al

Poster (2014, September 17)

Detecting of bad channels and artifacts for whole-night polysomnographic recordings is very time consuming and tedious. We therefore developed an automatic procedure to automatize this job.

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See detailMémoire épisodique et vieillissement normal : L'effet de la manipulation de la facilité de traitement perceptif en reconnaissance
Bastin, Christine ULg; Willems, Sylvie ULg

Poster (2014, September 16)

Etant donné que le vieillissement normal s’accompagne d’un déclin de la recollection tandis que la familiarité reste préservée, les tâches mnésiques facilitant l’utilisation de la familiarité ... [more ▼]

Etant donné que le vieillissement normal s’accompagne d’un déclin de la recollection tandis que la familiarité reste préservée, les tâches mnésiques facilitant l’utilisation de la familiarité permettraient de réduire les différences liées à l’âge en mémoire épisodique. Cette étude a évalué deux questions : 1) L’utilisation de la familiarité en reconnaissance peut-elle être favorisée en augmentant la différence de facilité de traitement perceptif (fluence) entre les items étudiés et les nouveaux items ? ; 2) Cette manipulation permet-elle de réduire l’effet de l’âge ? Deux tâches de reconnaissance ont été proposées à 24 participants jeunes et 24 participants âgés. Dans une tâche « sans recouvrement », les mots cibles et les mots nouveaux ne possédaient aucune lettre commune. L’exposition préalable aux mots cibles induisait donc une fluence sur les mots, mais également sur les lettres de sorte que la différence de fluence devenait un indice saillant et fiable pour distinguer cibles et distracteurs. Dans une tâche « avec recouvrement », les mots cibles et les mots nouveaux contenaient des lettres communes. Les résultats ont répliqué l’effet de l’âge sur la recollection (réponses Remember), avec préservation de la familiarité (réponses Know). Par ailleurs, nous observons 1) une amélioration de la discrimination dans la tâche « sans recouvrement », avec un plus grand nombre de reconnaissances correctes et moins de fausses reconnaissances (les deux basés sur la familiarité). De plus, 2) les différences liées à l’âge dans la capacité à discriminer cibles et distracteurs (reconnaissances correctes - fausses reconnaissances) étaient significativement réduites dans la tâche sans recouvrement par rapport à la tâche avec recouvrement. Ces données suggèrent que minimiser la similarité perceptive entre les mots cibles et les mots distracteurs, et donc augmenter la fluence, permet de réduire les différences liées à l’âge en reconnaissance en facilitant l’utilisation de la familiarité. [less ▲]

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See detailNear-death experiences in non-life-threatening events and coma of different etiologies.
Charland-Verville, Vanessa ULg; Jourdan, Jean-Pierre; Thonnard, Marie ULg et al

Poster (2014, September 16)

Near death experiences (NDEs) are increasingly being reported as a clearly identifiable physiological and psychological reality of clinical significance. Empirical studies of NDEs have mostly been ... [more ▼]

Near death experiences (NDEs) are increasingly being reported as a clearly identifiable physiological and psychological reality of clinical significance. Empirical studies of NDEs have mostly been conducted in patients with life threatening situations such as cardiac arrest [1-5] or (albeit more rarely) in patients with severe traumatic brain injury[6]. To the best of our knowledge, no study has formally compared the influence of the cause of coma to the intensity or content of the NDE. Using the Greyson NDE scale [7], the present retrospective study aimed at: (1) exploring the NDE intensity and content in “NDE-like” accounts following non-life-threatening events versus “real NDE” following coma; (2) comparing the “real NDE” characteristics according to the etiology of the brain damage (anoxic, traumatic or other) and; (3) comparing our retrospectively obtained data in anoxic coma to historical previously published prospectively collected post-anoxic NDEs. [less ▲]

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See detailRoughness evolution of sol-gel optical coatings by ion beam sputtering
Gailly, Patrick ULg; Dubreuil, Olivier ULg; Fleury-Frenette, Karl ULg

Poster (2014, September 16)

The surface roughness evolution of two silica-based sol-gel materials under 650 eV argon ion beam sputtering has been investigated. The liquid sol-gel solutions had been applied on silicon substrates ... [more ▼]

The surface roughness evolution of two silica-based sol-gel materials under 650 eV argon ion beam sputtering has been investigated. The liquid sol-gel solutions had been applied on silicon substrates using the dip coating technique and then thermally cured to obtain solid thin films. Their thickness had then been controlled over the samples surface using spectroscopic ellipsometry. The surface roughness of the sol-gel films has been measured using both interferometric profilometry and atomic force microscopy at different sputtering depths. Roughness increases significantly faster with sputtering depth in sol-gel layers than on bulk fused silica. Interestingly, the sputtering rates of the sol-gel layers are also observed to be much higher that of bulk fused silica. The development of micron scale holes with relatively stable interstices rules the surface roughness evolution. AFM measurements revealed a regular submicron scale lateral structure which nanometric amplitude is amplified under sputtering. [less ▲]

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See detailImpact of traps on the electrical characteristics of GeSn/Ge diodes
Baert, Bruno ULg; Gupta, Somya; Gencarelli, Federica et al

Poster (2014, September 15)

Germanium-tin alloys are currently receiving a lot of attention as materials for high performance MOSFET devices. Much interest is focused on the direct band gap for Sn concentrations above 8-10% and the ... [more ▼]

Germanium-tin alloys are currently receiving a lot of attention as materials for high performance MOSFET devices. Much interest is focused on the direct band gap for Sn concentrations above 8-10% and the achievement of high mobility values, which can be further increased by the strain due to the lattice mismatch with Ge or Si. GeSn is therefore expected to play a key role in the development of either source and drain stressors for Ge p-MOSFETs or for GeSn channel MOSFETs. However, despite recent tremendous progress in the growth of such materials, the impact of defects at the interface between Ge and GeSn has not been completely characterized. As the processing of diodes contains many of the steps necessary to the fabrication of MOSFET devices, we have investigated the effect of traps on the electrical characteristics of p-GeSn/n-Ge diodes, made from GeSn layers grown by CVD on Ge and in-situ doped with Boron. Using temperature-dependent current-voltage (I-V) and capacitance- voltage (C-V) measurements, we have calculated the ideality factor of the diodes, the activation energy of the reverse saturation current and the carrier concentration of the Ge substrate. In this work, based on the comparison with results obtained from numerical simulations, we discuss these characteristics in view of assessing the extent to which electronic trap states in these heterostructures affect their electrical properties. [less ▲]

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See detailInvestigation of the thermal and structural behavior of two lauric fats in bulk and oil-in-water emulsion states
Petrut, Raul Flaviu ULg; Anihouvi, Prudent Placide; Blecker, Christophe ULg et al

Poster (2014, September 14)

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See detailTranscriptomic characterization of pancreatic duct cells in adult zebrafish
Manfroid, Isabelle ULg

Poster (2014, September 13)

Transcriptomic characterization of pancreatic duct cells in adult zebrafish David Bergemann, Estefañia Tarifeño, Aurélie Ghaye, Marianne Voz, Bernard Peers and Isabelle Manfroid* Zebrafish Development and ... [more ▼]

Transcriptomic characterization of pancreatic duct cells in adult zebrafish David Bergemann, Estefañia Tarifeño, Aurélie Ghaye, Marianne Voz, Bernard Peers and Isabelle Manfroid* Zebrafish Development and Disease Models laboratory, Giga-Research, University of Liège, Belgium Loss of pancreatic beta cells is a hallmark of diabetes. To compensate the resulting deficit of insulin and maintain glucose homeostasis, beta cell replacement by transplantation or regeneration constitute promising strategies. Studies have shown beta cell neogenesis, though by mechanisms not fully understood. These beta cells can derive from replication of pre-existing beta cells1 or from conversion of alpha cells2. Besides these mechanisms, pancreatic ducts have also been hypothesized to contain progenitors/precursors for beta cell regeneration. However, the existence of such adult progenitors remains controversial. Zebrafish has been extensively used as a model for embryogenesis and regeneration. Their beta cells are very similar in development and function to their mammalian counterpart. Indeed, as in mammals, beta cells control glucose homeostasis in adult zebrafish. But, in contrast to mammals, adult zebrafish display the remarkable capacity to spontaneously, efficiently and rapidly regenerate their beta cells after ablation. The origin of these new beta cells is unknown but strong evidences collected in larvae argue in favour of a ductal origin. First, larval pancreatic ducts give rise to endocrine cells in normal condition and in response to the absence of beta cells4. Second, mutant larvae of the ductal marker sox9b harbour defects specifically in duct formation, and beta cell regeneration is impaired5. Here we show that adult pancreatic duct cells can give rise to beta cells. To get insight into this process, we established the transcriptome of adult duct cells by RNA-Seq. Comparison with the transcriptome of the other pancreatic cells identified known and novel ductal markers. Adult duct cells are also characterized by strong expression of embryonic pancreatic progenitor markers (sox9b, pdx1, nkx6.1 and components of the Notch pathway...). Future comparative analyses with ductal cells isolated from zebrafish in different conditions of beta cell (neo)genesis will help identify novel important players in beta cell formation from the ducts as well as regulators of beta cell regeneration. 1. Dor Y et al.(2004) Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Nature 2. Thorel F et al. (2010) Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss. Nature 3. Moss JB et al. (2009) Regeneration of the pancreas in adult zebrafish. Diabetes 4. Ninov N et al. (2013) Metabolic regulation of cellular plasticity in the pancreas. Curr. Biol. 5. Manfroid I et al. (2012) Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration. Dev. Biol. [less ▲]

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See detailRebuilding the educational system in post-conflict context in Kosovo - Survival of the Unmik’s provisions to support the living together through primary education
Pierre, Alexia ULg

Poster (2014, September 12)

In the aftermaths of the Cold War, the International Community adopts the objective of Education for All. Indeed, Education appears as a tool of social and economical development worldwide and supports ... [more ▼]

In the aftermaths of the Cold War, the International Community adopts the objective of Education for All. Indeed, Education appears as a tool of social and economical development worldwide and supports global development. Education allows individuals to take part effectively and actively in the collective life of the society they belong to. Soon after the adoption of this objective, the war is identified as the major obstacle in the fulfillment of this objective of Education for All. The international stakeholders takes their stand in favor of the continuation of the schooling during protracted crisis and wars, what lead to the education in emergency. This education has to spread values, behaviors and attitudes that attack the roots of the conflict. An objective of peace building and conflict prevention is since added to education. In a same temporality, the Kosovo Albanians were submitted to a segregationist policy headed by the Government of Milosevic, the Republika Srpska. This Government aims to make the Kosovo an unmixed territory, inhabited only by Serbs. Kosovo Albanians are rejected from all the state positions and state-owned entreprises. Education appears as a convergent point of the communities antagonisms and a critical issue between Serbs and Albanians in Kosovo. The freedom of being educated in their mother tongue is denied to the Kosovo Albanians through the policy of the Republika Srpska. The Kosovo Albanians react in setting up a parallel education system, in which virtually all the Albanian children intend school from 1992 to 1998. This parallel education system can be seen as a strong element of the pacific resistance of the Kosovo Albanians and a factor of social strength. This parallel educational system collapsed in 1998 due to the war situation in Kosovo. During this internal armed conflict, violations of the Law of war and of the International humanitarian Law are so huge that Nato intervenes through a military air campaign. This military intervention is immediately followed by a civil administration mission, the Unmik, whose mandate is to normalize the life of the inhabitants of Kosovo. Unmik is a restorative model not dedicated to the war victims. The restorative aspects for victims can be seen in the reinstallation of the Kosovo Albanians as full-right citizens. When arriving in Kosovo, Unmik faces two systems of education. Unmik’s first objective is to rebuild a unified education system in Kosovo. Along with objectives of democratization and modernization of a system inherited from a communist model. This intervention is an opportunity to promote and support peace education and conflict prevention through education, in echo with the latest international provisions on education. To achieve this objective of peace building and conflict prevention through education, the pedagogical methods, the contents of teaching and the structure of the education system has to be reformed and restructured. My work aims to explore the reconstruction process of the education system in Kosovo and the operationalization of the provisions that turn education as a tool of peace and conflict prevention, in the primaries schools of Prishtina. My approach is qualitative, inspired by the systemic perspective. The aim of this research is not to evaluate the results of the new Kosovo education system. This work is designed to explore the process of this post-war reconstruction, and to understand how international provisions come within this process and are effectively implemented in the primaries schools. The research question is on the present survival and effectiveness of these provisions in the primaries schools of Prishtina. This work was done first by a confrontation between a corpus of international normative texts on education and a legislative and normative corpus of local texts on education. This first step was dedicated to the selection of the special provisions relative to education as a tool of peace building. It appears that Civic Education and History are the two main teachings in the broadcasting of elements that helps to create a social cohesion. The tools for the data collection were based on the elements selected through this bibliographical research, as the analysis table of the empirical datas. Standardized questionnaires has been completed by teachers in History and Civic Education, and by Directors of the primaries school of Prishtina. Interviews with Kosovo people who were involved in the rebuilding process of the education system were carried out. The results highlights that International Community massively imposed this new model of education, what is visible through the identical profiles of all the schools that participated to the research. It appears that such an intervention is limited by the specificities and the reality of the Kosovo, which were not taken into consideration enough to make this new education system fit well to the very specific aspects of Kosovo. The question of the adaptability of such a model, created out of the Kosovo and without sufficient rely on the local population, is raised through this study. Indeed, the actual education system in Prishtina faces huge challenges in terms of equipment, funding and space. These challenges were not met by the Unmik’s work and are still existing, when the legislative and normative frame is continually developed in respect of the international developments in the matter. Indeed, fundamental education has to be a way to get competencies leading to a democratic citizenship, instead of a way to acquire only school basic knowledge. The potential in peace building and conflict prevention of the present education system in Kosovo is not able to be fully developed. If all the international provisions are well translated into the local provisions and operationalized in the primaries schools, the system has reached its limits and is not able to overcome its numerous challenges. Moreover, there is still two independent systems of education in Kosovo, and the education dedicated to the peace building and conflict prevention reaches only children intended schools of the official system. Serbs children are still educated in the same perspective than during the war. In a long terme perspective, the Kosovo education system will not be able to produce the expected outcomes. This contains risks of resurgence of the conflict. [less ▲]

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See detailPsychological processes mediating the link between depression severity and the level of behavioural activation: Assessment of a conceputal model
Wagener, Aurélie ULg; Baeyens, Céline; Blairy, Sylvie ULg

Poster (2014, September 12)

INTRODUCTION. Depression is a common psychopathology characterized by a decrease of the level of behavioural activation (BA) that is the level of engagement in activities. The relationship between ... [more ▼]

INTRODUCTION. Depression is a common psychopathology characterized by a decrease of the level of behavioural activation (BA) that is the level of engagement in activities. The relationship between depression and BA is well-established in the scientific literature. According to theoretical models of depression, psychopathology is also characterized by biases in five sets of psychological processes (PP): self-image (e.g. self-esteem), negative repetitive thoughts (e.g. ruminative thoughts), experiential avoidance (e.g. mindfulness), environmental satisfaction (e.g. environmental reward observation) and emotional regulation (e.g. distress tolerance). Therefore, the present study aims to identify PP mediating the relationship between depression and the level of BA. In order to reach this objective, a conceptual model is tested. METHOD. 361 adults (x ̅ age=36, σ=15), from clinical and non-clinical settings, fulfilled a series of questionnaires assessing several variables: sociodemographic data, depression, anxiety, level of BA and the above-cited five sets of PP. Covariance structure analysis will be conducted in order to test the conceptual model mentioned above. RESULTS. Some preliminary results are presented. As expected, a negative significant correlation between depression and BA (r=-.66, p<.01) have been highlighted. Negative significant correlation appeared between BA and the measured PP: self-esteem (r=-.27, p<.01), ruminative thinking (r=-.53, p<.01), environmental reward observation (=-.13, p<.05). Positive significant correlations appeared between BA and mindfulness (r=.49, p<.01) and distress tolerance (r=.26, p<.01). DISCUSSION. The preliminary results are in line with our hypotheses. Indeed, the level of BA is negatively linked to PP usually biased in depression such as self-esteem. The mediating role of these PP between depression and the level of BA will be discussed. [less ▲]

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See detailEvidence for Holocene bottom-currents erosion in the Western Gulf of Corinth, Greece
Beckers, Arnaud ULg; Beck, Christian; Hubert, Aurelia ULg

Poster (2014, September 10)

The Gulf of Corinth, Greece, is connected to the Ionian Sea through a 62 m deep sill. Strong tidal currents have been measured above this sill, what could potentially induce bottom-current erosion in the ... [more ▼]

The Gulf of Corinth, Greece, is connected to the Ionian Sea through a 62 m deep sill. Strong tidal currents have been measured above this sill, what could potentially induce bottom-current erosion in the Gulf. Seismic reflexion data allowed us to identify this present-day expected seafloor erosion in a wide area, as well as erosional unconformities and a wide channel between 100 and 300 m below sea level. These features highlight the possible occurrence of strong bottom-currents since the last sea level rise. [less ▲]

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See detailCFD Comparison of hydrodynamics between BIOSTAT® Cultibag STR and standard Stirred Cell Culture Bioreactors
Calvo, Sébastien ULg; Delafosse, Angélique ULg; Crine, Michel ULg et al

Poster (2014, September 10)

Single-use bioreactors have been increasingly used for animal cell cultures on microcarriers in screening experiments. Because of their manufacturing process, the shape of these kinds of vessels such as ... [more ▼]

Single-use bioreactors have been increasingly used for animal cell cultures on microcarriers in screening experiments. Because of their manufacturing process, the shape of these kinds of vessels such as the BIOSTAT® Cultibag STR one, is limited. For example, technical constraints on weld seams limit angles to 30° and no baffle may be installed. The shape of standard re-usable vessels may thus not be exactly reproduced and the flow structure may thus significantly differs from the flow structure in standard bioreactors. [less ▲]

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See detailStructural and functional analysis of the HMA4 protein
Lekeux, Gilles ULg; Laurent, Clémentine ULg; Damblon, Christian ULg et al

Poster (2014, September 09)

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See detailBioRefine Project: Detection of bioavailability of Metallic Trace Elements in soils by the use of microbial biosensors
Tarayre, Cédric ULg; Hurdebise, Quentin ULg; Fischer, Christophe ULg et al

Poster (2014, September 09)

Zinc, lead and cadmium are the main Metallic Trace Elements (MTEs) found in soils contaminated by the mining industry in Europe. MTEs are spread in the environment because of the disruption of ... [more ▼]

Zinc, lead and cadmium are the main Metallic Trace Elements (MTEs) found in soils contaminated by the mining industry in Europe. MTEs are spread in the environment because of the disruption of biogeochemical cycles caused by human activities. Due to their low mobility and biodegradability, they accumulate in soils where they are strongly bound to particles. It has become necessary to understand interactions between MTEs and the environment and to implement remediation actions. This work is focused on remediation monitoring techniques by using whole cell microbial biosensors able to detect zinc, lead and cadmium. Biosensors provide a signal in response to the bio-available concentration in MTEs, which are valuable for the design of efficient techniques involving bioremediation. Whole cell biosensors used in this work are based on Escherichia coli strains carrying a fluorescent reporter system. The reporter element contains a promoter sensitive to MTEs and a gene coding for the Green Fluorescent Protein (GFP). MTEs activate the synthesis of GFP, which is a very stable protein, causing the accumulation of GFP inside the cells. Then, fluorescence can be measured by flow cytometry. In this study, two biosensors were investigated: E. coli pPzraPgfp and E. coli pPzntAgfp. The last strain provided a linear response to zinc up to 20 mg/l and a curvilinear response to cadmium up to 0.15 mg/l. No detection was highlighted regarding lead. In practical cases, soils and wastes are contaminated by several types of MTEs. Consequently, combined contaminations were also tested. This work allowed highlighting that the strain E. coli pPzntAgfp can be used to assess the bioavailability of cadmium in soils, although the experimental procedure must be improved. This work is supported by the BioRefine Project, a European project in which various member states focus on recovery of inorganics from organic wastestreams. We gratefully acknowledge the INTERREG IVB NWE programme, which financed the BioRefine Project (ref. 320J-BIOREFINE). [less ▲]

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See detailAGE-RELATED MACULAR DEGENERATION (AMD): FROM METABOLOMICS APPROACH TO THE INHIBITION OF PDK AS A NEW THERAPEUTIC TARGET
Arslan, Deniz ULg; Pirotte, Bernard ULg; De Tullio, Pascal ULg et al

Poster (2014, September 09)

Age-related Macular Degeneration (AMD) is a leading cause of vision loss in the western world among people aged 50 or older. 90% of all vision loss due to AMD results from the exudative form, which is ... [more ▼]

Age-related Macular Degeneration (AMD) is a leading cause of vision loss in the western world among people aged 50 or older. 90% of all vision loss due to AMD results from the exudative form, which is characterized by choroidal neovascularization (CNV). Age-related changes that induce pathologic CNV are incompletely understood. A successful application of anti-VEGF approaches in the clinic is obviously a turning point in AMD treatment. Nevertheless, despite such important advances, critical issues remain to be addressed. To better understand the aetiology of this pathology, we used and improved a murine model of laser-induced choroidal neovascularization and applied a 1H NMR metabolomics study. This approach leads to the emergence of different putative biomarkers and to the validation of the CNV model for an experimental study of AMD. Among these “biomarkers”, lactate appears to be clearly involved in the development of AMD. The modulation of their plasma concentration by treatment of the animals with synthetic compounds and more specifically Pyruvate Dehydrogenase Kinase inhibitors (PDK) significantly decrease the impact of laser induced CNV. Starting from these results, the development of new PDHK inhibitors could open the way to innovative treatment opportunities in AMD disease [less ▲]

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See detailEffects of Dietary Protein Content on Second Reproductive Cycle of Local Rabbit Does and Their Litters.
SAIDJ, D.; AIN BAZIZ, H.; DAHMANI, Y. et al

Poster (2014, September 08)

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See detailVery deep images of the disc around beta Pictoris at Lp
Milli, J.; Absil, Olivier ULg; Mouillet, D. et al

Poster (2014, September 08)

We present observations of the innermost regions of the disc around beta Pictoris performed with VLT/NaCo in the Lp band. We combined seven deep datasets to retrieve the morphology of the disc down to 0 ... [more ▼]

We present observations of the innermost regions of the disc around beta Pictoris performed with VLT/NaCo in the Lp band. We combined seven deep datasets to retrieve the morphology of the disc down to 0.1arcsec. Our observations reveal intriguing ripples in the midplane of the disk at 0.5arcsec, as well as an overall bow of the disc spine, compatible with a disc slightly inclined with respect to edge-on scattering light anisotropically. Modelling suggests the presence of dust down to 10AU in order to explain the bright flux seen below 0.5 arcsec. [less ▲]

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See detailDesign of a fungal biofilm reactor for recombinant protein production from Aspergillus oryzae
Zune, Quentin ULg; Delepierre, Anissa ULg; Bauwens, Julien ULg et al

Poster (2014, September 07)

Fungi are microorganisms exhibiting high secretive power of various metabolites and have the ability to perform post-translational modifications during protein synthesis. In the field of fermentation ... [more ▼]

Fungi are microorganisms exhibiting high secretive power of various metabolites and have the ability to perform post-translational modifications during protein synthesis. In the field of fermentation industry, they are ideal hosts for secondary metabolites and recombinant protein production. At the industrial-scale, equipments usually required for solid-state or submerged fermentation of filamentous fungi have demonstrated their limitations in terms of productivity, mass transfers or products recovery (1, 2). Recently, fungal biofilm reactors were designed to combine advantages from submerged and solid-state culture and reveal their usefulness for greater secondary metabolites production relative to submerged culture conditions (3). In our work, we propose the design of a fungal biofilm reactor for a recombinant protein production from an Aspergillus oryzae strain containing a GFP reporter gene system under the control of a promoter specifically induced in solid-state conditions. The fungal biofilm reactor is composed of a metal structured packing, having the function of inert support for biofilm growth, immerged or aspersed by a liquid medium. Whereas recombinant protein production is not significantly different at the flask-scale between submerged and biofilm conditions, productivity is higher in the submerged conditions at the bioreactor-scale. Presence of recombinant proteins entrapped in the biofilm matrix highlights a diffusion constraint and a lower mass transfer in our fungal biofilm reactor. However, persistence of a free liquid biomass of low viscosity and fungal biomass retention on the support are attractive for the implementation of a continuous process in our fungal biofilm reactor. Further studies will consider a 2-D proteomic comparison of the extracellular medium from fungal biofilm reactor and submerged culture conditions in order to better understand proteins secretion and identify over-expressed proteins in biofilm conditions. [less ▲]

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See detailStudy of the conditions of conidiation for the bio-fungicide Pseudozyma flocculosa
ZAKI, Omran

Poster (2014, September 07)

Pseudozyma flocculosa is a useful fungus and is used in biological control of mildew. The reproduction of this fungus seems to be exclusively asexual by conidia’s production. The latter physiological form ... [more ▼]

Pseudozyma flocculosa is a useful fungus and is used in biological control of mildew. The reproduction of this fungus seems to be exclusively asexual by conidia’s production. The latter physiological form seems to be resistant to downstream process and storage conditions. Therefore, it represents the most commonly form used in agriculture as Fungicide. However, production of conidia in submerged culture is usually uncertain, often with very low yield. Despite the advantages that P. flocculosa offers in biological control, its use remains largely limited compared with that of chemical fungicides. This is probably due to the difficulty to produce mature conidia with high yields. In this work, our aim is to decipher developmental phases of conidia by this useful strain. We start by conidia production in submerged and semisolid fermentation. We then focus on comparative study of different kinds of obtained conidia. [less ▲]

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See detailObservational signatures of past mass-exchange episodes in massive binaries
Raucq, Françoise ULg; Rauw, Grégor ULg; Gosset, Eric ULg et al

Poster (2014, September 05)

Mass and momentum exchanges in massive binaries can produce several observational signatures, such as asynchronous rotation and altered chemical compositions, which remain once the stars detach again. We ... [more ▼]

Mass and momentum exchanges in massive binaries can produce several observational signatures, such as asynchronous rotation and altered chemical compositions, which remain once the stars detach again. We have started to investigate these effects for a sample of detached massive O-star binaries that are thought to have previously experienced a Case A Roche lobe overflow. Using phase-resolved spectroscopy, we perform the disentangling of the spectra of the two stars. The reconstructed primary and secondary spectra are then analyzed to determine a range of stellar effective temperatures and gravity, as well as rotational velocities. Using model atmosphere codes we also constrain the chemical composition of the components. In this contribution, we present the first results of our analyses of HD149404 (O7.5If + ON9.5I, P = 9.81 days). [less ▲]

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See detailPredictive microbiology combined with metagenomic analysis targeted on the 16S rDNA : A new approach for food quality
Delhalle, Laurent ULg; Ellouze, Mariem; Taminiau, Bernard ULg et al

Poster (2014, September 01)

The food spoilage process is mainly caused by alteration micro-organisms and classical culture-based methods may not be relevant to understand the modifications of the microbial ecology in food products ... [more ▼]

The food spoilage process is mainly caused by alteration micro-organisms and classical culture-based methods may not be relevant to understand the modifications of the microbial ecology in food products. Metagenomic analysis targeted on 16S ribosomal DNA can elucidate microbial community structures at a muche higher resolution than was previously possible. Combined with predictive microbiological models, a new approach was investigated to take into account bacterial populations dynamics in perishable foods under different environmental conditions. White pudding samples, a typical Belgian pork meat product, were packed under food wrap (atmospheric air condition). Durability studies were conducted at 4°C, 12°C and a dynamic temperature profile according to the NF V01-003 standards (4°C (1/3 of the shelf life) - 8°C (2/3 of the shelf life)) during 15 days. The effect of organic acids was also investigated using a lactic acid (1.8% w/w) treatment. At each day of the trials, classical microbiological (total flora) and 16S rDNA metagenomic analysis were carried out on all these samples. For the metagenomic analysis, a sequencing library was generated, targeting the V1-V3 region of the 16S rDNA. The two major bacterial populations were thus identified (Psychrobacter sp and Brochotrix thermosphacta) and predictive microbiology models used to assess the growth parameters. Cardinal parameters for temperature were collected on the two main bacterial species. The model was validated using the data obtained at a dynamic temperature profile. The results of the simulations for Psychrobacter sp and Brochotrix thermosphacta show a good compliance between predicted and observed data. Compared to culture based methods on selective media and previous independent culture techniques, metagenomic analysis combined with predictive microbiology gives more valuable information, and could be considered as a technological breakthrough to control the quality or for accurately determining shelf life. [less ▲]

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See detailI know what you did last summer - High resolution mapping of wild boar damages with drones
Michez, Adrien ULg; Morelle, Kevin ULg; Lehaire, François ULg et al

Poster (2014, September 01)

Wild boar ability to cause important damages to agricultural lands is not anymore to be demonstrated. These damages often raise conflicts between farmers and hunters due to the associated economic losses ... [more ▼]

Wild boar ability to cause important damages to agricultural lands is not anymore to be demonstrated. These damages often raise conflicts between farmers and hunters due to the associated economic losses. Objective and accurate method for real impact assessment of losses suffered by farmers is thus needed. Currently,in Wallonia damage assessment is performed from the ground by experts, asked to evaluate precisely the area impacted. This task is time and man power consuming. Moreover, damaged areas are often large and consequently, sampling is mandatory and can induce accuracy issues. Recent development of unmanned aerial vehicle (UAV / drone) toghether with lower cost and better accessibility for civil society and scientific community, makes now possible the use of this technology for wildlife researches. Through photogrammetric 3D reconstruction of the field crop, UAV can allow to objectively and precisely measure the area impacted by wild boar damages. In a context of increasing issues caused by wild boar to agriculture, and the need for stakeholders for accurate impact assessment, we tested an innovative approach to estimate more effectively the area impacted by wild boar. [less ▲]

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See detailDo the counting methods distort our perception of bivalve diversity through time?
Ros-Franch, Sonia; Cascales - Miñana, Borja ULg; Martínez-Pérez, Carlos

Poster (2014, September)

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See detailAutomatization of mirror - tracing skill in children with Developmental Coordination Disorder
Lejeune, Caroline ULg; Meulemans, Thierry ULg; Desmottes, Lise ULg et al

Poster (2014, September)

Aim: This study investigated the hypothesis of a skill automatization deficit in Developmental Coordination Disorder (DCD). Method: Thirty children (15 with DCD and 15 control children), aged between 7 ... [more ▼]

Aim: This study investigated the hypothesis of a skill automatization deficit in Developmental Coordination Disorder (DCD). Method: Thirty children (15 with DCD and 15 control children), aged between 7 and 12 years old, were administered the mirror-tracing task during two 10-trials sessions separated by one week. An auditory interference task was introduced at the end of the procedural learning phase to test skill automatization. Results: Interestingly, no between-group difference was revealed in learning and automatization measures except for a specific subgroup of DCD children (n=5) who experienced difficulties in skill automatization. Conclusion: The results of our preliminary study highlighted the heterogeneity of the deficit presented in DCD and they emphasized the importance to explore further the lack of automatization in DCD. [less ▲]

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See detailAssessment of a multiscale fatigue damage model associated with stress gradient effects
Duchene, Laurent ULg; Marmi, Abdeljalil ULg; Habraken, Anne ULg

Poster (2014, September)

The aim of this research work is to develop a finite element numerical tool able to predict accurately the fatigue life of mechanical components. These components can have complex geometries, they can be ... [more ▼]

The aim of this research work is to develop a finite element numerical tool able to predict accurately the fatigue life of mechanical components. These components can have complex geometries, they can be submitted to a complex loading, leading to a specific stress field with possible stress concentration. Additionally, the successive cycles of loading are not necessarily identical. It is expected that the numerical tool can handle these demanding constraints. In this respect, a multiaxial fatigue damage model was implemented in our home-made finite element code Lagamine. The finite element method permits to account for the actual geometry of the mechanical part and the loading for the stress computation in the whole structure. The formulation of the multiaxial fatigue model is able to capture: - The non linear damage accumulation for multiblock and variable cyclic loading, - The effect of the mean (hydrostatic) stress, - The effect of the cycles below the fatigue limit if the damage was previously initiated. Finally, the occurrence of stress concentration will significantly reduce the life time of the studied piece. However, it is well-known that the subsequent local degradation of the material will be partly compensated by an enhanced load carrying contribution of the surrounding material, favourably leading to a reduction of the crack propagation. The stress gradients computed with different techniques are incorporated in the model so as to account for such beneficial influence. The physical roots of this model depart from the mesoscopic length scale, where the damage evolution is related to the mesoscopic accumulated plastic strain. Therefore, the variables of the model are defined at both macroscopic and mesoscopic scales and a specific scale transition method was implemented, based on the well-known simplified Zarka method but used at the multiscale level. The predictive capabilities of this multiscale multiaxial model are assessed by means of comparison with the classical Lemaitre-Chaboche model (implemented in the same FE code with stress gradient effects). For both models, the material parameters were identified from SN tests on smooth specimens of Ti-6Al-4V alloy, while the predictions of the models are validated thanks to comparison with experimental tests on notched samples, with stress gradient effects. [less ▲]

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See detailUnveil the Mechanism of Action of Berenil, a DNA Binding Drug, on Trypanosoma cruzi
Zuma, Aline Araujo; Cavalcanti, Danielle Pereira; Thiry, Marc ULg et al

Poster (2014, September)

Detailed reference viewed: 9 (0 ULg)
See detailTHE BCCM/ULC CULTURE COLLECTION TO CONSERVE AND STUDY THE DIVERSITY OF (SUB)POLAR CYANOBACTERIA
Wilmotte, Annick ULg; Renard, Marine ULg; Kleinteich, Julia et al

Poster (2014, September)

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. A Quality Management System was implemented and is continuously improved since then ... [more ▼]

The BCCM/ULC public collection of (sub)polar cyanobacteria is funded since 2011 by the Belgian Science Policy Office. A Quality Management System was implemented and is continuously improved since then. An ISO9001 certificate was obtained for the public deposition and distribution of strains, as part of the multi-site certification for the BCCM consortium. BCCM/ULC is currently holding 134 cyanobacterial strains and the catalogue is available on http://bccm.belspo.be/catalogues/ulc-catalogue-search. Continuous maintenance of living cultures, some of which are also cryopreserved, ensure the preservation and the possibility to rapidly deliver strains to clients for fundamental and applied research. The collection includes 113 (sub)polar strains. In such extreme environments, cyanobacteria are important phototrophs and primary producers in terrestrial and freshwater ecosystems. The purpose of this collection is to gather a representative portion of the cyanobacterial diversity with different ecological origins (limnetic microbial mats, soil crusts, cryoconites, endoliths, etc.) and make it available for researchers to study the taxonomy, evolution, adaptations to environmental conditions, and genomic make-up. Thus, 102 cyanobacterial strains were isolated from the three main biogeographic zones of the Antarctic continent. In addition, 7 strains were isolated in Arctic biotopes and 4 from Siberian lakes. The molecular characterization is underway, on the basis of 16S rRNA and ITS sequences. Moreover, a Multilocus Sequence Analysis is tested on diverse strains to improve their systematics. The diversity encompasses the three main cyanobacterial orders: Chroococcales, Oscillatoriales and Nostocales. In addition, cyanobacteria are known to produce a range of secondary metabolites (e.g. alkaloides, cyclic and linear peptides, polyketides) with different bioactive properties (e.g. antibiotic, antiviral, antifungal, anticancer). The potential of the polar strains to produce cyanotoxins is currently studied by ELISA and the detection by PCR of genes involved in their production. Due to the geographic isolation and the strong environmental stressors of the habitat, the exploration of these metabolites in Antarctic cyanobacterial strains seems promising for biotechnology or biomedical applications. [less ▲]

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See detailCortical excitability dynamics of during sleep deprivation set PVT performance
Borsu, Chloé; Gaggioni, Giulia ULg; Ly, Julien ULg et al

Poster (2014, September)

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See detailModelling the development of potential spoilage and biopreservative microorganisms on precooked pasta in different conditions of temperature based on classical microbiology and 16S rDNA metagenomic
Gand, Mathieu ULg; Cargnel, Mickaël ULg; Kergourlay, Gilles ULg et al

Poster (2014, September)

An important way to prevent the spoilage of food is the respect of the cold chain during the storage. While the temperature instructions are generally respected during process and distribution of food ... [more ▼]

An important way to prevent the spoilage of food is the respect of the cold chain during the storage. While the temperature instructions are generally respected during process and distribution of food products, it is not always the case with the consumers. Indeed, a few persons reach the right temperature level required for a safe storage of foodstuffs in their refrigerator. Besides, the food can sometimes spend a few hours in ambient temperature between the buying in the supermarket and the storage in cold temperature. In this study, we propose to model the growth of microorganisms on precooked pasta, stored in different conditions of temperature that reflect the situations described above (constant 4°C, constant 8°C, constant 12°C, 1/3 4°C – 2/3 8°C, 1/3 4°C – breach during 4h at 20°C – 2/3 8°C and 1/3 8°C – breach during 4h at 20°C – 2/3 8°C. The product was surface inoculated with potential spoilage and biopreservative strains (Lactococcus piscium, Leuconostoc mesenteroides and Leuconostoc citreum). Analyses by classical microbiology and V1-V3 16S rDNA metagenomics were done each day until the out of date of the food matrix. The transition from 4 to 8°C and the breach at 20°C during 4h have clearly boosted the growth of the microorganisms. The metagenomic analysis was a powerful tool to follow separately each population in each condition of storage. The results of this communication show the importance of keeping the foodstuffs in 4°C or lower in the refrigerator with the goal to avoid the spoilage or the development of pathogens and the potential of metagenomics for selection of biopreservative strains. [less ▲]

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See detailAb-initio Study of Ferecrystals
Dewandre, Antoine ULg; Verstraete, Matthieu ULg

Poster (2014, September)

Ferecrystals are a new family of compounds first synthesized in 2007 by the group of D. Johnson at the University of Oregon. These materials consist of inter-growths of dichalcogenide and chalcogenide ... [more ▼]

Ferecrystals are a new family of compounds first synthesized in 2007 by the group of D. Johnson at the University of Oregon. These materials consist of inter-growths of dichalcogenide and chalcogenide layers, and can be written as [(M X)_{1+δ} ]_m [T X_2 ]_n where M = Sn, Pb, Sb, Ni and some rare earths; T = Ti, V, Cr, Nb and Ta; X= S and Se. The integers m and n denote the numbers of consecutive formula unit layers in the different components of the inter-growth. The δ parameter reflects the difference of the in-plane cell constants between components of the inter-growth. This family of nanostructured materials shows promising properties for thermoelectric devices. The compounds studied here are [(SnSe)_1.29 ]_{234} [M oSe_2 ]_1 . We performed structural characterisation and examined the transfer of charge at the interface between the two materials. We show that there is a depletion of charge at the interface between the two compo- nents of the heterostructures and that structural distortions of the SnSe layers in the supercell are similar to those observed in slab calculations. [less ▲]

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See detailRole of estradiol in the feminization of lordosis behavior
Trouillet, Anne-Charlotte ULg

Poster (2014, September)

The classic theory of mammalian brain and behavioral sexual differentiation holds that an organizational action of testosterone, secreted perinatally by the male’s testes, controls male-typical aspects of ... [more ▼]

The classic theory of mammalian brain and behavioral sexual differentiation holds that an organizational action of testosterone, secreted perinatally by the male’s testes, controls male-typical aspects of brain and behavioral development, whereas no active perinatal sex hormone signaling is required for female-typical differentiation. However, it has recently been shown using aromatase knock out (ArKO) mice, which are deprived of estradiol due to a targeted mutation in the aromatase gene, that estradiol may also actively contribute to the differentiation of female-typical aspects of behavior over a specific pre-pubertal period. Sexual behavior is a well known sexually dimorphic behavior, and thus provides a good model for studying the sexual differentiation of the brain. Female ArKO mice showed significantly lower levels of lordosis behavior than wild type controls following adult ovariectomy and treatment with ovarian hormones. This deficit was restored by treating female ArKO mice with estradiol benzoate between postnatal days 15 and 25. However, the mechanism by which estradiol acts prepubertally in female mice to feminize lordosis behavior remains to be elucidated. Therefore, in a first attempt, we will determine which neuropeptides are in particularly sensitive to estradiol action over the prepubertal period. We will focus on neuropeptides which have been shown to be implicated in lordosis behavior. Studies by the group of Micevych have proposed an important role for NPY and beta-endorphin (B-END) neurons in the arcuate nucleus (ARC) in the estradiol-mediated activation of lordosis behavior in female rats. An in vitro study recently showed that kisspeptin directly activates arcuate (ARC) B-END neurons. This is particularly interesting since unpublished results from our laboratory showed that Kp neurons are directly involved in the expression of lordosis behavior. We are thus currently determining the expression of NPY, B-END, and kisspeptin in the ARC as well as μ-opioid receptors MORs in the MPN over the prepubertal period and their possible modulation by prepubertal estradiol treatment. Interestingly, we also observed that our treatment may have an organizational effect on the regulation of energy balance. Indeed, administration of estradiol over the prepubertal period restores a normal weight in ArKO mice, which are normally obese in adulthood. It has been shown that ArKO mice present a lower spontaneous physical activity which can be one cause of their obesity. However, our treated mice did not show an increase in their locomotion behavior. These new observations suggest that estradiol during prepubertal development may have an additional role in the organization of brain circuits regulating the energy balance. [less ▲]

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See detailModelling the development of potential spoilage and biopreservative microorganisms on white pudding in different conditions of temperature based on classical microbiology and 16S rDNA metagenomic
Gand, Mathieu ULg; Cauchie, Emilie ULg; Kergourlay, Gilles ULg et al

Poster (2014, September)

An important way to prevent the spoilage of food is the respect of the cold chain during the storage. While the temperature instructions are generally respected during process and distribution of food ... [more ▼]

An important way to prevent the spoilage of food is the respect of the cold chain during the storage. While the temperature instructions are generally respected during process and distribution of food products, it is not always the case with the consumers. Indeed, a few persons reach the right temperature level required for a safe storage of foodstuffs in their refrigerator. Besides, the food can sometimes spend a few hours in ambient temperature between the buying in the supermarket and the storage in cold temperature. In this study, we propose to model the growth of microorganisms on white pudding, stored in different conditions of temperature that reflect the situations described above (constant 4°C, constant 8°C, constant 12°C, 1/3 4°C – 2/3 8°C, 1/3 4°C – breach during 4h at 20°C – 2/3 4°C and 1/3 4°C – breach during 4h at 20°C – 2/3 8°C. The product was surface inoculated with potential spoilage and biopreservative strains (Raoultella terrigena, Serratia quinivorans, Carnobacterium maltaromaticum, Lactobacillus oligofermentans, Lactobacillus nenjiangensis, Lactobacillus fuchuensis, Leuconostoc mesenteroides, lactococcus lactis and Lactobacillus graminis). Analyses by classical microbiology and V1-V3 16S rDNA metagenomics were done each day until the out of date of the food matrix. The transition from 4 to 8°C and the breach at 20°C during 4h have clearly boosted the growth of the microorganisms. The metagenomic analysis was a powerful tool to follow separately each population in each condition of storage. The results of this communication show the importance of keeping the foodstuffs in 4°C or lower in the refrigerator with the goal to avoid the spoilage or the development of pathogens and the potential of metagenomics for selection of biopreservative strains. [less ▲]

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See detailSELF BROADENING COEFFICIENTS AND IMPROVED LINE INTENSITIES FOR THE v7 BAND OF C2H4 NEAR 10.5 µm, AND IMPACT ON ETHYLENE RETRIEVALS FROM JUNGFRAUJOCH SOLAR SPECTRA
Vander Auwera, Jean; Fayt, André; Tudorie, Marcella et al

Poster (2014, September)

Relying on high-resolution Fourier transform infrared (FTIR) spectra, the present work involved extensive measurements of individual line intensities and self-broadening coefficients for the ν7 band of ... [more ▼]

Relying on high-resolution Fourier transform infrared (FTIR) spectra, the present work involved extensive measurements of individual line intensities and self-broadening coefficients for the ν7 band of 12C2H4. The measured self-broadening coefficients exhibit a dependence on both J and Ka. Compared to the corresponding information available in the latest edition of the HITRAN spectroscopic database, the measured line intensities were found to be higher by about 10 % for high J lines in the P branch and lower by about 5 % for high J lines of the R branch, varying between these two limits roughly linearly with the line positions. The impact of the presently measured line intensities on retrievals of atmospheric ethylene in the 949.0–952.0cm 1 microwindow was evaluated using a subset of ground-based high-resolution FTIR solar spectra recorded at the Jungfraujoch station. The use of HITRAN 2012 with line intensities modified to match the present measurements led to a systematic reduction of the measured total columns of ethylene by -4.1 +/- 0.1 %. [less ▲]

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See detailNumerical modelling of centrifugal settlers based on experimental data
Macher, Robert; Pfennig, Andreas ULg

Poster (2014, September)

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See detailAutomatic biorythms description from actigraphic data
González y Viagas, Miguel ULg; Ly, Julien ULg; Gaggioni, Giulia ULg et al

Poster (2014, September)

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See detailSimulation based design of extraction columns for aqueous two-phase systems
Schmidt, Markus; Wessling, Matthias; Pfennig, Andreas ULg

Poster (2014, September)

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See detailUsa de programa APS en el analysis de la forma de la arcada dental.
LEWY, Stéphanie; LIMME, Michel ULg; BRUWIER, Annick ULg

Poster (2014, September)

Detailed reference viewed: 12 (0 ULg)
See detailIntroducing the Belgian Science and Technology Studies Network (BSTS)
Meyers, Gert; Van Oudheusden, Michiel ULg; Thoreau, François ULg et al

Poster (2014, September)

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See detailEvaluation of Temocillin for treatment of nosocomial infections
LAYIOS, Nathalie ULg; CIUTEA, Mirela ULg; LONGUEVILLE, Manon et al

Poster (2014, September)

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See detailEffect of AMF enrichment with 13C-­‐triacylglycerol tracers on its thermal and structural behavior
Danthine, Sabine ULg; Vors, C.; Michalski, M-C

Poster (2014, September)

Detailed reference viewed: 10 (0 ULg)