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See detailHigher male than female recombination rate in cattle is controlled by genetic variants effective in both sexes
Kadri, Naveen Kumar ULg; Harland, Chad ULg; Coppieters, Wouter ULg et al

Poster (2015, May 05)

We herein study genetic recombination in three dairy cattle populations from France, New-Zealand and the Netherlands. We apply a new phasing algorithm extracting familial information suited for large half ... [more ▼]

We herein study genetic recombination in three dairy cattle populations from France, New-Zealand and the Netherlands. We apply a new phasing algorithm extracting familial information suited for large half-sib families to reconstruct haplotypes and detect cross-overs (CO). The software is robust to genotyping and map errors. We identify more than 2,000,000 CO events in sperm cells transmitted by 3008 sires to 94,603 offspring, and more than 500,000 CO events in oocytes transmitted by 11,497 cows to 25,390 offspring. When measured in identical family structures, the average number of CO in males (24.0) was found to be larger than in females (21.8). In males, recombination rates were higher closer to telomeres whereas in females, recombination rates dropped at both centromeres and telomeres (probably as a result of lower informativity). The heritability of the global recombination rate (GRR) was close to 0.20 in males and to 0.08 in females. Genetic correlation ranged from 0.38 to 0.69 depending on the population, indicating that shared variants are influencing GRR in both genders. Haplotype-based genome-wide association studies revealed four genome-wide significant QTL, including two previously identified ones (involving REC8 and RNF212). For all QTLs, there was a positive correlation between haplotype effects across sexes, ranging from 0.35 to 0.68. We selected two reference panels of respectively 122 and 215 bulls sequenced at cover > 15x to impute variants in the New-Zealand and French populations. All variants identified by next-generating sequencing in 5 Mb windows encompassing the QTL peaks were imputed with Beagle in order to perform a sequence-based association study. For three QTLs, we identified missense mutations in genes known to be involved in meiosis among the most significantly associated variants. These variants were perfectly associated with the haplotypes underlying the QTL effects. The variant identified in RNF212 had already been reported, whereas missense mutations in MLH3 (N408S) and HFM1 (S1189L) are new findings. Surprisingly, variants previously identified in REC8 did not capture the QTL effect whereas variants in RNF212B, PPP1R3E, BCL2L2, HOMEZ and PABPN1 had much stronger association with the phenotype. The three missense mutations were significant in both genders with two of them accounting for approximately 10% of the genetic variance in males (the allelic substitution effect being approximately equal to one additional CO per genome). Our results are very different from reports of recombination in other species. For instance, in human, recombination rate is higher in females, distinct variants affect recombination rate in males and females and the genetic correlation is close to 0 whereas in cattle, we observed a higher recombination rate in males controlled by shared variants effective in both sexes. [less ▲]

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See detailUse of GEOS-Chem for the interpretation of long-term FTIR measurements at the Jungfraujoch and other NDACC sites
Mahieu, Emmanuel ULg; Bovy, Benoît ULg; Bader, Whitney ULg et al

Poster (2015, May 04)

We present recent and ongoing investigations using 3D CTM GEOS-Chem model simulations for the interpretation of long-term FTIR measurements performed at selected NDACC sites.

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See detailCarbon dioxide, a cheap bio-sourced building block for cyclic carbonates and non-isocyanate polyurethanes
Gennen, Sandro ULg; Alves, Margot ULg; Tassaing, Thierry et al

Poster (2015, May 03)

Due to concerns about global warming combined with the decrease of fossil resources, new carbon feedstocks that are abundant, renewable, non-toxic, inexpensive and environmentally friendly must be ... [more ▼]

Due to concerns about global warming combined with the decrease of fossil resources, new carbon feedstocks that are abundant, renewable, non-toxic, inexpensive and environmentally friendly must be explored to produce chemicals. Besides the valorization of bio-based raw materials, the chemical transformation of carbon dioxide into added-value products has gained interest in both academia and industry. To date, the chemical fixation of CO2 onto epoxides with the formation of cyclic carbonates is one of the most promising ways to valorize CO2 at an industrial scale. Indeed, cyclic carbonates find applications as electrolytes in lithium ion batteries, as aprotic polar solvents or as useful intermediates for polycarbonates. Cyclic carbonates also react with primary amines to produce 2-hydroxyethylurethane. This reaction can be extrapolated to the synthesis of non-isocyanate polyurethanes (NIPU) by polyaddition of bifunctional cyclic carbonates with diamines.5 This study aims (i) at developing a new highly efficient organocatalyst for the synthesis of cyclic carbonates under mild experimental conditions and (ii) their valorization as monomers to produce non-isocyanate polyurethanes. First, we have identified a bicomponent organocatalyst, composed of a judicious combination of an organocatalyst and an activator, for the very fast synthesis of cyclic carbonates from CO2 and epoxides under very mild reaction conditions. Kinetics of reactions were followed by online Raman spectroscopy measurements under pressure. NMR titrations were realized to evidence the mechanism of activation of this novel organocatalytic system that will be discussed in detail in this talk. The second objective relies on the development of new efficient organocatalysts for the synthesis of high molar masses NIPUs in short reaction times. Organic compounds interacting with the cyclic carbonate by hydrogen bonding were identified and their catalytic activity was demonstrated for model compounds. [less ▲]

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See detailCharacterization of almond kernel oils of five almonds varieties cultivated in Eastern Morocco
Houmy, Nadia ULg; Mansouri, Farid; Ben Moumen, Abdessamad et al

Poster (2015, May 03)

This study focuses on characterization of almond kernel oils extracted mechanically from five sweet almond varieties Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil ... [more ▼]

This study focuses on characterization of almond kernel oils extracted mechanically from five sweet almond varieties Marcona, Fournat, Ferragnes, Ferraduel and Beldi), cultivated in eastern Morocco. Oil content, physicochemical parameters, triacylglycerol and fatty acid compositions were determined. Analyzed oils showed low acidity values that range between 0.77 – 0.88 %, peroxide values range between 6.43 – 16.39 meq/kg and Iodine values range between 98.42 – 103.90%. The principal fatty acid of almond kernel oils is oleic acid (C18:1); oils of Ferragnes-Ferraduel and Beldi varieties show higher values of C18:1 respectively of 72.87 and 71.62 %, however Fournat almond kernel oil shows the lowest content of C18:1 (63.54%). HPLC analysis of triglycerides was carried out, and results show that analyzed almond kernel oils are characterized by the dominance of trioleylglycerol (OOO) that contents range between a minimum of 31.48 % for Fournat’s oil and 43.82% for Ferragnes-Ferraduel’s oil. The oxidative stability of almond kernel oils was determined by rancimat tests as the induction period (IP, h recorded by a 743 Rancimat apparatus Metrohm, Switzerland). Results show that stability, of almond kernel oils is clearly influenced by the almond variety; Oxidative stability of tested almond kernel oils ranged between an IP = 20.28 h for Marcona oil and an IP =27.55 h for Ferragnes-Ferraduel. [less ▲]

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See detailStudy of family drawings and self-esteem in institutionalized children
Gallo, Alicia ULg; Simar, Angélique; Blavier, Adelaïde ULg et al

Poster (2015, May)

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See detailLarge-scale polarization alignments of quasars in the JVAS/CLASS 8.4-GHz surveys
Pelgrims, Vincent ULg; Hutsemekers, Damien ULg

Poster (2015, May)

We analyse the large sample of polarization measurements of the flat-spectrum radio sources of the JVAS/CLASS 8.4-GHz surveys compiled by Jackson et al. (2007). We tested the uniformity of the ... [more ▼]

We analyse the large sample of polarization measurements of the flat-spectrum radio sources of the JVAS/CLASS 8.4-GHz surveys compiled by Jackson et al. (2007). We tested the uniformity of the polarization position angles for a wide range of angular (2D) and comoving (3D) separations and studied the several subsamples, dividing the main sample of 4155 sources regarding their object type (QSO, galaxies, radio sources,...). We found regions of the sky of about 20 degree radius in which quasars (only) have correlated polarization position angles. Those regions coincide with the regions of alignment at optical wavelength pinpointed in 1998 by Hutsemékers. [less ▲]

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See detailLIFE CYCLE ASSESSMENT OF ANTHROPIC WATER
Groslambert, Sylvie ULg; Léonard, Angélique ULg

Poster (2015, May)

In its Regional Policy Statement for 2009-2014, the Walloon Government (Belgium) undertakes to make Wallonia a model of good governance. Applying this principle in the field of the environment needs to be ... [more ▼]

In its Regional Policy Statement for 2009-2014, the Walloon Government (Belgium) undertakes to make Wallonia a model of good governance. Applying this principle in the field of the environment needs to be based on a continuous, objective and strict evaluation of the evolution of the state of environmental components (air, water, soil, biodiversity, etc.), the pressures to which they are subjected, the resultant impacts and the measures taken to improve living conditions within our Region. Within this framework, the General Operational Direction of Agriculture, Natural Resources and Environment (DGARNE) realises reports on the state of the environment in Wallonia, in close collaboration with universities and research centres. A chapter is dedicated to the efficient use of water resources, including a life cycle assessment of anthropic water and rain water harvesting. [less ▲]

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See detailRegiospecific radiolabeling of Nanofitin on Ni Magnetic Beads with 18F-FBEM and in vivo PET studies
Dammicco, Sylvestre ULg; Lemaire, Christian ULg; Cinier, Mathieu et al

Poster (2015, May)

Nanofitins(NFs) are a small, single chain and cysteine-free protein able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide ... [more ▼]

Nanofitins(NFs) are a small, single chain and cysteine-free protein able to selectively bind a defined biological target. They derive from Sac7d bacterial protein family and are highly stable over a wide range of pH (0-13) and temperature (Tm ~80°C). Their extreme stability, low cost and high tolerability for chemical coupling make NFs an interesting alternative to antibodies. The aim of this study was to develop the first synthesis of a radiolabeled NF since no in vivo biodistribution kinetic studies have been published. [less ▲]

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See detailThe effects of perinatal exposure to polychlorinated biphenyls on hippocampal neurogenesis
Pinson, Anneline ULg; Parent, Anne-Simone ULg; chatzi, christina et al

Poster (2015, May)

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See detailGeoarchaeology of the ancient city of Utica (Tunisia) and evolution of the palaeoenvironment of the Medjerda delta
Pleuger, Elisa ULg; Abichou, Hakim; Gadhoum, Ahmed et al

Poster (2015, May)

Phoenician Utica remains today largely unknown, as is its role in the Phoenician expansion in the western Mediterranean. Aristotle and Pliny the Elder mention Utica as a maritime and port city and ... [more ▼]

Phoenician Utica remains today largely unknown, as is its role in the Phoenician expansion in the western Mediterranean. Aristotle and Pliny the Elder mention Utica as a maritime and port city and estimate its origin around the 11th c. BC. However, in the present state of research, no archaeological evidence is earlier than the 9th c. BC, and the location of the Phoenician and Roman port infrastructures remains unknown. Today, the ancient city is located on a promontory in the heart of the Medjerda delta, 10 km inland. This project proposes an interdisciplinary effort to understand the Medjerda delta landscape changes during the Holocene. It starts from an archaeological problem and proposes the contribution of geoarchaeology to the understanding of the relationship between ancient societies and their environment. The fluvial palaeoenvironments and sedimentary processes are studied through the mechanical extraction of cores (15-20 m deep) to reach the early Holocene. Selected sediment samples are then studied in laboratory, using different and complementary approaches. The location of port infrastructures will bring initial answers to the question of the foundation of the city. The study of river palaeoenvironments of the Medjerda delta during the Holocene aim at a better understanding of the nature of the settlement, as well as the function of the city of Utica over time. This study will also assess the impact of the ancient city on the environment and understand how the city adapted to the mobility of this Mediterranean delta. Furthermore, the analysis of sedimentary processes causing the filling of the harbour basin will lead to speculation about the causes of the abandonment of the structures and more generally the decline of the city in favor of Carthage. It will also examine whether natural or anthropogenic factors have influenced this deltaic progradation over the centuries. [less ▲]

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See detailDerjaguin-Broekhoff-de Boer analysis of adsorption in very disordered mesopores using probabilistic models
Gommes, Cédric ULg

Poster (2015, May)

Our current ability to produce mesoporous materials with ordered morphology has raised fascinating questions about the impact of geometrical disorder on adsorption and desorption states [1]. Many recent ... [more ▼]

Our current ability to produce mesoporous materials with ordered morphology has raised fascinating questions about the impact of geometrical disorder on adsorption and desorption states [1]. Many recent works have investigated the role of mild elements of disorder, such as local constriction or corrugation superimposed to otherwise geometrically ideal cylindrical pores [2,3]. These works have notably shown that elements of disorder may act as nucleation sites and destabilize vapor-like metastable states. The relevance of these perturbation-like results to fundamentally disordered materials, such as gels, is unclear. In particular, do vapor-like metastable states exist at all in this type of very disordered material? In the present communication, we address this question using probabilistic models to investigate the role of disorder. We generalize the classical Gaussian field models of porous materials [4] and use them to analyze adsorption and desorption in the Derjaguin-Broekhof-de Boer approximation. Our approach differs from earlier contributions in that both the adsorbent and the adsorbate are described in terms of probabilities [5]. This enables us to analyze the adsorbate configuration in very disordered solids using a low-dimensional yet realistic configuration space. We notably show that vapor-like metastable states are unlikely in gel-like disordered materials. [1] D.Wallacher, N. Künzner, D. Kovalev, N. Knorr, K. Knorr, Capillary condensation in linear mesopores of different shape, Phys. Rev. Lett. 92 (2004) 195704; [2] B. Coasne, A. Galarneau, F. Di Renzo, R.M.J. Pellenq, Effect of morphological defects on gas adsorption in nanoporous silicas, J. Phys. Chem. C 111 (2007) 15759; [3] C.J. Gommes, Adsorption, capillary bridge formation, and cavitation in SBA-15 corrugated mesopores: A Derjaguin-Broekhoff-de Boer analysis, Langmuir 28 (2012) 5101-5115; [4] R.J. Pellenq, P. levitz, Capillary condensation in a disordered mesoporous medium: A grand canonical Monte Carlo study, Molecular Physics 100 (2002) 2059;[5] C.J. Gommes, A.P. Roberts, in preparation. [less ▲]

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See detailUse of 454 pyrosequencing protocol for the assessment of cyanobacterial diversity
Stelmach Pessi, Igor ULg; De Carvalho, Pedro; Laughinghouse, Haywood Dail et al

Poster (2015, May)

A pilot run was carried out on Antarctic microbial mat samples in order to test different protocols for 454 pyrosequencing and bioinformatic analyses. An artificial community was assembled using22 ... [more ▼]

A pilot run was carried out on Antarctic microbial mat samples in order to test different protocols for 454 pyrosequencing and bioinformatic analyses. An artificial community was assembled using22 cyanobacterial strains in two different abundance distributions. Bioinformatic analyses were carried out on the artificial communities using variations of the mothur and UPARSE pipelines. Moreover, different DNA extraction methods, technical replicates and biases arising from direct barcoded primer amplification were assessed using five microbial mat and soil crust samples. A great variation in richness estimates was observed using different bioinformatic pipelines, with UPARSE generating the most consistent results. On the other hand, community structures observed using different DNA extraction protocols were statistically similar. The same was observed for the technical replicates. Moreover, the use of barcoded primers did not influence the observed community structure. [less ▲]

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See detailThe role of sea ice in the carbon cycle of Polar Seas: 1D to 3D modelling
Moreau, S.; Vancoppenolle, M.; Delille, Bruno ULg et al

Poster (2015, May)

Sea ice participates actively in the biogeochemical cycle of carbon of Polar Oceans, yet to which extent is not clear. We investigated the processes that govern sea ice carbon dy- namics in Polar Regions ... [more ▼]

Sea ice participates actively in the biogeochemical cycle of carbon of Polar Oceans, yet to which extent is not clear. We investigated the processes that govern sea ice carbon dy- namics in Polar Regions through 1D to 3D modelling developments. First, we constrained all major physical and biogeochemical processes with respect to CO2 dynamics (carbon- ate chemistry, biological activity, ikaite (CaCO3•6H2O) precipitation and dissolution and ocean-ice-air CO2 fluxes) in a one-dimensional sea ice model. According to our model, the CO2 budget is driven by the CO2 uptake during ice growth and release by brine drainage, whereas other processes such as brine-air CO2 fluxes, despite significant, are secondary. Subsequently, based on these preliminary conclusions, we evaluated the role of sea ice in the carbon dynamics of Polar Oceans by using an ocean-ice coupled Global Earth System Model. Carbon dynamics (e.g. ocean-atmosphere CO2 fluxes) are driven by the contribution of sea ice growth regions in the Arctic Ocean (mainly the Siberian coast) and sea ice melt regions in the Southern Ocean (off the coast of Antarctica). In addition, the production of deep waters is low in the Arctic Ocean but significant in the Southern Ocean. Therefore, sea ice only contributes to the deep water export of carbon in the Southern Ocean. The role of sea ice in the biogeochemical cycle of carbon is significant and its representation by Global Earth System Models should be improved. [less ▲]

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See detailLife cycle assessment of biobased chemical building blocks made from European waste streams
Groslambert, Sylvie ULg; Léonard, Angélique ULg

Poster (2015, May)

Many drivers exist to persuade EU businesses to embrace the opportunities of the circular economy. However, the challenges faced by companies, particularly among SME’s, in the drive for resource ... [more ▼]

Many drivers exist to persuade EU businesses to embrace the opportunities of the circular economy. However, the challenges faced by companies, particularly among SME’s, in the drive for resource efficiency include access to funding, knowledge and capability, and ability to implement cost-effective technological solutions. It was these factors that inspired the creation of the ReNEW network. ReNEW, ‘Resource innovation Network for European Waste’, is a €5 million project funded by the Interreg IVB North West Europe scheme aiming to increase cooperation between research and business endeavours to create value from waste by optimising novel technologies for extracting materials from waste and their reuse in the supply chain. ReNEW also aims to inform local, national and European policy makers, and to share transnational best practice and improve specific support to meet the innovation needs of the waste sector. Within the frame of this project, several technologies are investigated by partners to improve the valorisation of organic wastes in chemicals such as the production of furfural (C5H4O2 - CAS 98-01-1) by the chemical hydrolysis of contaminated/hazardous woody biomass. [less ▲]

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See detailPb-based versus Sn-based perovskite solar cells: Toxicity and environmental burden
Babayigit, Aslihan; Dinh Duy Thanh, ULg; Ethirajan, Anitha et al

Poster (2015, May)

Organometal halide perovskites have rapidly evolved into strong contenders to compete with silicon in the quest for low-cost photovoltaics, with their added value being solution-processability. Their ... [more ▼]

Organometal halide perovskites have rapidly evolved into strong contenders to compete with silicon in the quest for low-cost photovoltaics, with their added value being solution-processability. Their primary drawback, however, is that so far the presence of lead (Pb) is required to obtain the highly favorable electro-optical properties of the most successful perovskite crystals such as CH3NH3PbI3. Together with their tendency to degrade under the influence of humidity, and the corresponding disintegration of the unit cell, this implies that Pb compounds can be released into the environment upon failure of a perovskite module. As already known from literature, Pb is a rather toxic element causing irreversible neurological, nephrotic and hepatic damage. Hence, finding a non-harmful alternative metal, exhibiting similar electro-optical characteristics in the resulting perovskite crystal, could be the solution to improve and ultimately commercialize perovskite-based solar cells. Tin (Sn), also being a group IV metal, has been deemed the most appropriate alternative. However, Sn is also enlisted as a harmful chemical. Animal and human volunteer studies have shown that toxicity symptoms like fever, nausea, nephropathy, etc. emerge upon excessive uptake, raising question marks regarding the suitability of Sn as a more environmentally friendly alternative to Pb in perovskite solar cells. This contribution aims to make a first step towards the assessment of the environmental burden of both Pb and Sn based solar cells in the form of a toxicity study. Well-established aquatic model organisms are exposed to the appropriate degradation products, according to well-defined guidelines of the Organization for Economic Co-operation and Development (OECD). This allows a systematic comparison of Sn and Pb-containing decayed compounds regarding their potentially harmful effects on the environment, and sheds light onto the applicability of both corresponding perovskite families in large-scale photovoltaic systems. [less ▲]

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See detailCOMPARISON OF DIABETES CONTROL ONE YEAR AFTER GASTRIC BYPASS AND MAGENSTRASSE AND MILL PROCEDURES
SCHLECK, Michael ULg; KOHNEN, Laurent ULg; DE FLINES, Jenny ULg et al

Poster (2015, May)

Bariatric surgery has become a main therapy of type 2 diabetes in the obese population. The best surgical procedure to achieve diabetes control remains debated. Gastric bypass would be the preferred ... [more ▼]

Bariatric surgery has become a main therapy of type 2 diabetes in the obese population. The best surgical procedure to achieve diabetes control remains debated. Gastric bypass would be the preferred option according to its incretin stimulating potential. We compared in this study gastric bypass surgery to a pure restrictive procedure in terms of diabetes control at 1 year. [less ▲]

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See detailEvaluation of the effectiveness of simulation of cardiac arrhythmias in children
Bragard, Isabelle ULg; Seghaye, Marie-Christine ULg; Baugnon, Thomas et al

Poster (2015, May)

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See detailStrategies of regioselective radiolabeling of Nanofitin binder for imaging
Goux, Marine ULg; Dammicco, Sylvestre ULg; Becker, Guillaume ULg et al

Poster (2015, May)

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as fluorine-18, zirconium-89 or gallium-68. Despite their ... [more ▼]

Recently, new strategies emerged in the field of monoclonal antibodies radiolabeling for PET imaging with the use of positron emitter such as fluorine-18, zirconium-89 or gallium-68. Despite their important role in the therapeutic world, antibodies have many disadvantages related to their structure. Moreover, conjugation of chelating agent often occurs on lysines, which is non-regioselective and leads to a heterogeneous mixture of products. In addition, the slow clearance of antibodies can be a problem to obtain a good contrast when they are used in imaging. To address these different limitations, we developed a chemistry-free chelating system consisting of a highly phosphorylatable peptide tag fused genetically to a Nanofitin. A specific phosphorylation step, with the alpha subunit of the casein kinase II, generates a nanocluster of 4 phosphates that can interact strongly with metal ion like zirconium or gallium. This strategy has already demonstrated its powerfulness for the stable and specific anchoring of protein on zirconium phosphonate-based microarray [1]. Considering this tag has been created to specifically anchoring protein on zirconium phosphonate-based microarray, we are currently working on a sequence derived from calcium-binding proteins to chelate specifically lanthanides[2]. As described by Pardoux et al.[3], our strategy is to functionalize this sequence with a phosphate nanocluster able to chelate zirconium or gallium. Our first results shown that a mono-phosphorylatable tag phosphorylated in vitro is able to chelate terbium(III) with a lower affinity than the wild type. Considering that terbium(III) is bigger than gallium(III) or zirconium(IV), we can suppose that the cage obtained is too small but suitable for other ions. In order to validate our hypothesis, we have planned to radiolabel those tags and determine their affinity for gallium(III). In order to validate the use of Nanofitin as a potent alternative tool for in vivo imaging, we have made biokinetic studies in mice with PET and MRI imaging. In these studies, we have radiolabelled with fluorine-18 a Nanofitin and we are currently making use of its specific binding to a cell-surface receptor to target a very precise cell population by using an animal model. Once the phosphorylatable tag optimized for regioselective radiolabelling and the Nanofitin targeting validated in an animal model, the next steps will be to combine these two approaches: we will fuse genetically the tag to the specific Nanofitin, radiolabel it with gallium-68 and perform the biokinetic study of this new radiopharmaceutical product. [1] M. Cinier, M. Petit, F. Pecorari, D. R. Talham, B. Bujoli, and C. Tellier, “Engineering of a phosphorylatable tag for specific protein binding on zirconium phosphonate based microarrays.,” J. Biol. Inorg. Chem., vol. 17, no. 3, pp. 399–407, Mar. 2012. [2] L. J. Martin, “Development of Lanthanide-Binding Tags (LBTs) as powerful and versatile peptides for use in studies of proteins and protein interactions,” 2008. [3] R. Pardoux, S. Sauge-merle, D. Lemaire, P. Delangle, L. Guilloreau, J. Adriano, and C. Berthomieu, “Modulating Uranium Binding Affinity in Engineered Calmodulin EF-Hand Peptides : Effect of Phosphorylation,” PLoS One, vol. 7, no. 8, 2012. [less ▲]

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See detailComparison of individuals’ susceptibility to false memory induced by both DRM and misinformation paradigms involving emotional material
Martial, Charlotte ULg; Dehon, Hedwige ULg

Poster (2015, May)

False memories induced by the DRM procedure (“Deese, Roediger and McDermott”; Deese, 1959; Roediger & McDermott, 1995) or the misinformation procedure (in which a person’s recollection of a witnessed ... [more ▼]

False memories induced by the DRM procedure (“Deese, Roediger and McDermott”; Deese, 1959; Roediger & McDermott, 1995) or the misinformation procedure (in which a person’s recollection of a witnessed event is altered after exposure to misinformation about this event; Loftus et al., 1978) are due to errors in source monitoring processes (e.g., Johnson et al., 1993) and one might expect the correlation between these false memories quite positive. However, the few laboratory studies comparing the DRM paradigm and the misinformation paradigm show small (Zhu et al., 2013) or no correlation (Ost et al., 2013) between the false memories elicited by these procedures but these studies vary in terms of methodological details. For instance, false memories from the misinformation procedure involved emotional content while those from the DRM procedure only included neutral materials. This study investigated the relationship between false memories induced by two different paradigms (a DRM task and a misinformation procedure) both involving an emotional material. Participants (N = 154) completed an emotional variant of the DRM (neutral, positive and negative lists) and the misinformation (neutral, positive and negative images) procedures and their performances on both tasks were compared. Although both paradigms reliably induced false memories in participants, our analyses revealed only a marginally weak positive correlation (r = .147, p = .051) between misinformation and DRM false memories using emotional variants. These results support the idea that DRM and misinformation false memories are underpinned by (at least in part) different mechanisms and that the previous mixed results were not due to the specific content of the DRM or the misinformation task used. [less ▲]

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See detailEffects of a walk phase at the warm up onset on physiological and behavioural parameters of ridden horses (Equus caballus)
Jacquot, Marion; Grosjean, Adeline; Emrot, Cassandre et al

Poster (2015, May)

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See detailImproving proviral integration site detection with high throughput sequencing
Durkin, Keith ULg; Artesi, Maria ULg; Rosewick, Nicolas et al

Poster (2015, May)

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See detailAnatomic features underlying wood density, in 110 rainforest tree species from central Congo basin
de Haulleville, Thalès ULg; Rousseau, Mélissa; Morin-Rivat, Julie ULg et al

Poster (2015, May)

Investigate the influence of fiber thickness and vessel diameter on the wood density in 110 rainforest tree species, and the relationships between wood density, wood water content and shrinking ratio.

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Dekkers, Charlotte; DARFOUF, Rajae ULg et al

Poster (2015, May)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 117 strains have been currently confirmed as M. audouinii. Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (64%, p< 0,0001) with a sex-ratio M/F of 1.97. Data concerning the geographic origin of the family have been obtained in only 33,6% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (43,6%) suggesting bias in the data collection. The geographic origin of the remaining group includes several African countries such as Congo (20,61%), Guinea (12,8%) and Burundi (5,12%). The genotypic analysis led to the distinction of 6 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 96 strains, well distributed in different Belgium locations. Two other groups of three strains each were close to the major group but the analysis of the spectral superposition showed some differences between these groups. The two last groups were clearly different from the major group but species identification was confirmed by ITS sequencing. Conclusion The results of the genomic analysis by Diversilab, show that several groups of M. audouinii isolates co-exist in Belgium providing evidence of genetic heterogeneity. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as the age or ethnical origin. ________________________________________ [less ▲]

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See detailPRESPHOTO project - Preservation of microalgae in the BCCM collections
Crahay, Charlotte ULg; Deprez, Karolien; Vanormelingen, Pieter et al

Poster (2015, May)

The implementation of reliable preservation technologies of the biological resources is crucial for the management of the Biological Ressources Centers. PRESPHOTO, a BRAIN.be project, aims to develop and ... [more ▼]

The implementation of reliable preservation technologies of the biological resources is crucial for the management of the Biological Ressources Centers. PRESPHOTO, a BRAIN.be project, aims to develop and optimize new and cost-effective preservation techniques of photosynthetic microalgae (diatoms and cyanobacteria) in the two BCCM collections, BCCM/DCG and BCCM/ULC. This is a critical factor for the future growth and valorisation of these collections. Firstly, the traditional two-step cryopreservation technique will be adapted to photosynthetic microalgae strains. In particular, we will examine the effects of the culture conditions, the type and concentration of cryoprotectants, and the preservation temperature on the survival of microalgae. The encapsulation/dehydration technique as alternative to the two-step cryopreservation method will be also evaluated. Moreover, an independent validation of the developed protocols will be performed by the Culture Collection of Algae and Protozoa (UK) (subcontractor). Secondly, we will perform genome resequencing of selected strains to investigate the genetic changes induced by different cryopreservation techniques. Finally, a high-quality genomic DNA bank will be established and validated. [less ▲]

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See detailMapping Posidonia oceanica meadows through time A story of precision, evaluation and fragmentation
Abadie, Arnaud ULg; Jousseaume, Matthieu; Lejeune, Pierre et al

Poster (2015, May)

Over the last decades, the interest in mapping Posidonia oceanica beds has increased along with the improvement of the equipment’s precision of data acquisition. In Calvi Bay (Corsica, France) the meadows ... [more ▼]

Over the last decades, the interest in mapping Posidonia oceanica beds has increased along with the improvement of the equipment’s precision of data acquisition. In Calvi Bay (Corsica, France) the meadows cover an area of about 5 km² and are found at a depth ranging from 3 m to 37 m. The availability of three distinct datasets for 1997, 2002 and 2010 allowed to assess changes in the patchiness of the meadows in the bay and to investigate evolution of maps precision through a surface analysis via GIS software. Thus, three maps were elaborated combining aerial photographs and side scan sonar images. The meadows percentage of cover through time was assessed using four bathymetric sections: 0-10 m, 11-20 m, 21-30 m and 31-40 m. Differences in the patchiness of P. oceanica meadows between 1997 and 2010 appear to be moderate (less than 3 %) in the sections 0-10 m and 11-20 m and then greatly increase with depth: 24 % at 21-30 m and 39 % at 31-40 m. This amazing regression seems hardly natural and unlikely given the slight quantity of human activities that can cause damages on the P. oceanica meadows of the Calvi Bay. These results are likely to be mainly due to the improvement of precision and resolution of the aerial photographs (5 m in 1997, 0.8 m in 2002 and 0.5 m in 2010) and sonar images (5 m in 1997, 3 m in 2002 and 0.5 m in 2010). An issue of habitat determination (human vs instrumental) linked with the method adopted for mapping can also cause differences in the percentage of cover. Given the different accuracy among the three maps, the real regression and fragmentation of P. oceanica meadows could be hardly assessed. However, in several areas where the human activities are important, a clear regression or even a disappearance of the meadows has been observed. It is obvious that the last maps are more accurate than the previous ones and, thus, the former can be used for management purpose as well as for study on the patchiness; however, they still keep uncertainty no matter which method is used to create them. [less ▲]

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See detailBiosensor based on optical fibers
Lismont, Marjorie ULg; Vandewalle, Nicolas ULg; Joris, Bernard ULg et al

Poster (2015, May)

Medical diagnosis and biotechnology progresses are strongly dependent on the development of sensing devices, which, ideally, would allow the reliable detection of very low amounts of biological species in ... [more ▼]

Medical diagnosis and biotechnology progresses are strongly dependent on the development of sensing devices, which, ideally, would allow the reliable detection of very low amounts of biological species in various environments. In addition to these requirements, the detection tools would also be easy to use and would rapidly response. Fluorescent based biosensors fulfil most of these characteristics. In our work, the intersection between two crossed optical fibers is used as the basic unit of an original optical biosensor. As illustrated by figure 1, one optical fiber is used to carry probe molecules and excite fluorescence while the second one is devoted to carry the target species and collect the optical signal arising from the species interacting at the node. The advantages of our set-up over traditional optical sensors are no surface functionalization, use of low amounts of biological species, limitation of the denaturation risk, ease to use and low detection threshold. The developed biosensor is validated on two systems. The first one is a fluorescent calcium indicator, Oregon green 488 BAPTA-2, whose optical emission signal is affected by Ca2+ ions concentration. The second one is based on Rh-Con A and FITC-Dextran complex for which the FRET phenomenon is affected by glucose concentration. In both cases, the results are in agreement with the ones obtained in cuvettes attesting the efficiency of the sensing device. We also show a prototype of a multichannel device composed of multiple crossed optical fibers which are used as species and light carriers. [less ▲]

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See detailMania following acute decompensation in ornithine transcarbamylase deficiency.
JACQUINET, Adeline ULg; SIRRS, Sandra; MATTMAN, Andre et al

Poster (2015, May)

Background Ornithine transcarbamylase deficiency is the most common urea cycle disorder and presents an X-linked pattern of inheritance. Both males and females may be affected with variation in severity ... [more ▼]

Background Ornithine transcarbamylase deficiency is the most common urea cycle disorder and presents an X-linked pattern of inheritance. Both males and females may be affected with variation in severity and age of onset. Psychiatric symptoms, including episodic psychosis, atypical depression, confusion, erratic behavior or delirium, are possible presentations of late-onset disease. Manic behaviors have previously been reported with hyperammonemia induced by valproic acid. Usually, psychiatric behaviors cease with normalization of ammonia levels. Case report We report a family with two males with confirmed late-onset OTC deficiency (and other adult males with unexplained lethal encephalopathy) due to a missense mutation in OTC (c.119G>A; p.R40H). One 29-year-old male individual, with no psychiatric history, presented hypomanic symptoms with a significant shift from his personality in the days following his first major episode of acute decompensation. Ammonia levels were measured as 245, 30, 11, 106, 20, 38 and 17 micromol/L on days 2, 5, 8, 9, 9, 9, and 10, respectively, following the acute crisis onset. Symptoms of vomiting, confusion, tremors and loss of consciousness stopped at day 5. Hypomanic symptoms were noted from day 5 and were finally controlled with long-acting quetiapine ten days after normalization of serum ammonia levels (daily levels were normal from day 10 to day 21). CT scan of the brain was normal. Conclusion Illustrated by this case report, occurrence of mania secondary to a hyperammonic crisis may be the consequence of dysregulated neurotransmission balance in brain, which can persist after normalization of serum ammonia levels. [less ▲]

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See detailIn silico screening to predict chondrocyte hypertrophy using a semiquantitative gene network model
Kerkhofs, Johan ULg; Leijten, Jeroen; Luyten, Frank et al

Poster (2015, April 30)

PURPOSE: In development, chondrocyte hypertrophy is a crucial and well-studied step in endochondral ossification. Hypertrophy may also play a role in pathophysiological processes, including osteoarthritis ... [more ▼]

PURPOSE: In development, chondrocyte hypertrophy is a crucial and well-studied step in endochondral ossification. Hypertrophy may also play a role in pathophysiological processes, including osteoarthritis. We employ a computational approach to estimate the effect of individual factors in this complex process. METHODS: We have combined information gleaned from a high number of publications on chondrocyte differentiation into a gene regulatory network of 46 factors and over 150 interactions. This network can estimate the stability of proliferative chondrocytes/permanent cartilage (stable state with SOX9 activity) and hypertrophic chondrocytes (stable state with RUNX2 activity) by employing 2 measures. A first measure is a Monte Carlo analysis that assesses stability in the face of random initial conditions, the second modifies stable states to estimate the sensitivity to perturbation. RESULTS: For each factor, these qualitative measures are calculated in silico under knockout and overexpression conditions and compared to the wild type situation. This enables screening of the effects of all incorporated factors on cartilage homeostasis, differentiation and pathogenesis via the initiation of hypertrophy. Indeed, our gene network analysis indicated multiple candidate genes for the development of osteoarthritis. Factors that amplify the SOX9 attractor basin include TGFβ, PPR, IGF-I, and PKA. The presence of RAS, IHH, GLI2 and FGF is required for the Runx2 stable state. Using a literature study, we corroborated several of the proposed factors. CONCLUSIONS: In silico screening of overexpression and knockout presents a novel strategy to improve bone and cartilage tissue engineering approaches, and can be used to propose a list of putative therapeutic targets for e.g. osteoarthritis. [less ▲]

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See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULg; Géron, Bénédicte; Dekkers, Charlotte et al

Poster (2015, April 28)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 97 strains have been currently confirmed as M. audouinii. Preliminary analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (84%) with a sex-ratio M/F of 1.95. Data concerning the geographic origin of the family have been obtained in only 45.8% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (77%) suggesting bias in the data collection. The geographic origin of the remaining group (23%) includes several African countries. The genotypic analysis led to the distinction of 3 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 85 strains, well distributed in different Belgium locations. The last group contains only one strain but this strain was significantly different from the two other variants. Conclusion The automated typing DiversiLab® system proved to be an easy and efficient method to investigate the molecular epidemiology of dermatophytes infections. These preliminary results show that, through Belgium, several groups of isolates co-exist for M. audouinii providing evidence of genetic heterogeneity. At this time all epidemiological informations have not yet been assessed while 35 strains of M. audouinii remain to be analysed genotypically to give definitive conclusions. [less ▲]

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See detailEpidemiology and clinical reporting of candidaemia in Belgium : a national prospective study (TANSIR trial)
Trouvé, Charlotte; Blot, Stijn; Hayette, Marie-Pierre ULg et al

Poster (2015, April 26)

Objectives The aim of this multicenter study was to gather epidemiological data on candidemia in the Belgian population. Another goal was to determine the time in real life setting for reporting to the ... [more ▼]

Objectives The aim of this multicenter study was to gather epidemiological data on candidemia in the Belgian population. Another goal was to determine the time in real life setting for reporting to the treating physicians of the species involved and its antifungal susceptibility. Methods Prospective study in 29 Belgian hospitals. From March 1st, 2013 till February 28, 2014 the first Candida isolate from each episode of candidemia was included. Identification and susceptibility testing were performed according to local procedures and isolates were sent to the National Reference Lab with a completed case report form. Species identification was checked by MALDI-TOF mass spectrometry (MS) and ITS sequencing in case no reliable identification was obtained by MS. Antifungal susceptibility testing was performed according to EUCAST guidelines. The total number of patient admissions and hospitalization days during the study period was retrieved from each hospital. Results 341 isolates were retrieved from 325 patients (53.2% male, median age 66 years, range 1-94 years) admitted to the ICU (34.4%), medical wards (30.8%), surgical wards (15.2%), onco-haematology (10.6%), pediatrics (3.0%), neonatology (1.7%) and other wards (4.3%). The mean incidence rate of candidemia was 0.42 per 1,000 admissions (range 0.07 to 1.44) and 0.60 per 10,000 patient days (range from 0.11 to 2.03). Candida albicans was the main cause of candidemia (51.9%), followed by Candida glabrata (26.7%), Candida parapsilosis (9.9%), Candida tropicalis (4.4%), Candida guilliermondii (2.6%), Candida dubliniensis (1.5%), Candida lusitaniae (1.2%), Candida krusei (1.2%) and Candida metapsilosis (0.6%). Overall resistance to fluconazole was 6.7% and to anidulafungin 0.6% (2 C. glabrata isolates were echinocandin resistant). Resistance to amphotericin B was detected in 1 C. tropicalis isolate, all C. albicans, C. glabrata and C. parapsilosis isolates remained susceptible to this drug. Resistance to fluconazole ranged from 3.5% in C. albicans, 8.6% in C. glabrata, 5.6% in C. parapsilosis to 35.7% (5/14 isolates) in C. tropicalis. These five C. tropicalis isolates showed cross resistance to voriconazole and posaconazole. MIC values for caspofungin ranged from <0,016 to >8mg/L, with MIC50 of 0.06mg/L and MIC90 of 0.25mg/L. The median time between blood sampling and positivity of the blood culture bottle was 37h07min (Q1-Q3: 25h42min-54h28min). The median time between blood culture positivity and reporting of isolate identification and susceptibility to the treating physician was 29h58min (Q1-Q3: 23h21min-40h34min) and 59h34min (Q1-Q3: 48h28min-75h20min) respectively. Conclusions A large variation in the incidence of candidemia among Belgian hospitals was observed. Resistance to azole drugs remained low but emerging resistance to these drugs among C. tropicalis was noted. Resistance to echinocandins remains rare in Belgian Candida isolates. These data will be further analyzed in order to evaluate the influence of the identification and susceptibility testing method on the time to report results to the treating physicians. [less ▲]

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See detailEvaluation of a commercially developed semi-automated PCR-surface enhanced raman scattering assay for the detection of Candida species in blood
HAYETTE, Marie-Pierre ULg; WERY, Marie ULg; BOREUX, Raphaël ULg et al

Poster (2015, April 25)

Objectives Microbiological diagnosis of invasive candidiasis is still dependent on culture-based methods. The use of beta-D-glucan antigen detection is included in the EORTC microbiological diagnostic ... [more ▼]

Objectives Microbiological diagnosis of invasive candidiasis is still dependent on culture-based methods. The use of beta-D-glucan antigen detection is included in the EORTC microbiological diagnostic criteria but is rarely available in the clinical labs. On the other hand, PCR-based methods lack standardization. The RenDx Fungiplex® is a new commercially available semi-automated PCR SERS assay designed for the detection of Aspergillus sp. and Candida sp. including the differentiation of resistant strains as C. glabrata, C. krusei and A. terreus. This study was performed for sensitivity and reproducibility testing of the method on 8 different Candida species. Methods The study was conducted on EDTA-blood collected from a healthy donor. Blood samples were spiked with 10 Candida reference strains: C. albicans ATCC 10231, C. albicans NEQAS 1206 and C. albicans NEQAS 2359; C. glabrata ATCC 90030 ; C. krusei ATCC 6258 ; C. tropicalis NEQAS 1036 ; C. guillermondii NEQAS 1035 ; C. parapsilosis ATCC 22019 ; C. lusitaniae NEQAS 1511 and C. dubliniensis IHEM 14280. Spiked samples were diluted at final concentrations ranging from 1 CFU/mL to 1000 CFU/mL. Cultures on Sabouraud dextrose agar were performed in parallel to control yeasts dilutions. DNA extraction was performed by using proteinase K-based method followed by purification on QIAcube automate. The RenDx Fungiplex®kit (Renishaw) was used for the amplification process and the final detection was processed on the SP-1000 sample analyzer. Reproducibility testing was performed on the three C. albicans reference strains by repeating each test 5 times. Results A total of 142 samples were included in the study. A sensitivity of 10 CFU/mL was reached for C. glabrata, C. krusei, C. tropicalis, C. dubliniensis spiked samples while C. lusitaniae and C. tropicalis performed better at 1 CFU/mL. The three tested reference C. albicans strains and C. guillermondii gave the lowest sensitivity (100 CFU/mL). The reproducibility of the assay was 96% Conclusion RenDx Fungiplex®kit allows the detection of the most frequent Candida species responsible for invasive candidiasis in spiked blood samples. The sensitivity of the test is comprised between 10 and 100 CFU/mL for most Candida sp. and reproducibility is very high. This evaluation allows us to consider this commercial kit for inclusion in a clinical study on invasive candidiasis in comparison with non-molecular diagnostic assays. [less ▲]

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See detailStudy of a new splice variant of Neuropilin-1: antagonistic functions in the regulation of cancer progression?
Hendricks, Céline ULg; Janssen, Lauriane; Delcombel, Romain et al

Poster (2015, April 22)

Neuropilin-1 (NRP1) is a transmembrane glycoprotein and a co-receptor for several growth factors, for example some variants of the Vascular Endothelial Growth Factor A (VEGF-A). It largely contributes to ... [more ▼]

Neuropilin-1 (NRP1) is a transmembrane glycoprotein and a co-receptor for several growth factors, for example some variants of the Vascular Endothelial Growth Factor A (VEGF-A). It largely contributes to the regulation of angiogenesis but also to cancer formation. NRP1 can be considered as a proteoglycan as glycosaminoglycans side chains can be added on serine 612. Currently, six splice variants of NRP1 have been described. An additional form was recently identified in our laboratory. Depending upon the cell types, it represents 20-30% of the total amount of NRP1. As compared to the full size NRP1 (NRP1-FS), 7 amino acids are deleted. As the missing sequence is located 2 amino acids downstream of the Ser612 required for glycosaminoglycans addition, this process could be somehow affected and the function of the protein could be modified. The glycosylation of NRP1-FS and -Δ7 was analyzed in different cells overexpressing each isoform. Western blotting analyses suggested that NRP1-Δ7 was less glycosylated than NRP1-FS. Prostate cancer cells (PC3) were engineered to express NRP1-FS or –Δ7 only in the presence of doxycycline. The migration of these cells was analyzed by scratch assay, with or without doxycycline in the medium. As compared to controls and to NRP1-FS-expressing cells, production of NRP1-Δ7 was linked to a reduction of cell migration. A DNA dosage showed that NRP1-FS enhanced cell proliferation, while NRP1-Δ7 reduced it. Tumor growth was assessed in vitro by a culture in soft agar. As compared to control conditions, expression of NRP1-FS by doxycycline increased colonies formation. By contrast, NRP1-Δ7 inhibited colonies number, suggesting an inhibition of tumorigenesis by this variant. As PC3 cells express basal level of endogenous NRP1, this suggests some competitive inhibition of NRP1 functions by NRP1-Δ7. Finally, the function of each variant was investigated in vivo in a model of injection in the flanks of nude mice of PC3 cells conditionally expressing NRP1-FS or -Δ7. As compared to the control, NRP1-FS increased tumor size and weight. By sharp contrast, the expression of NRP1-Δ7 was associated with a reduction of tumorigenicity. Cells with forced expression of NRP1-Δ7 also developed fewer blood vessels as compared to the control. These results suggest that NRP1-Δ7 have an antagonistic action on cancer formation and angiogenesis. [less ▲]

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See detailSedimentary impacts of recent moderate earthquakes in different settings in the Western Gulf of Corinth, Greece
Beckers, Arnaud ULg; Mortier, Clément; Beck, Christian et al

Poster (2015, April 21)

11 short gravity cores retrieved in the Western Gulf of Corinth, Greece, allowed identifying event deposits whose age ranges were compared to an updated earthquakes catalogue for the area. 210Pb-derived ... [more ▼]

11 short gravity cores retrieved in the Western Gulf of Corinth, Greece, allowed identifying event deposits whose age ranges were compared to an updated earthquakes catalogue for the area. 210Pb-derived age-depth curves show that the majority of the event deposits may have been triggered by earthquakes. These results show that moderate earthquakes (Mw ~6.0-6.5) may significantly impact different marine settings, from shallow shelves (70-100 m deep) to the basin floor (330 m deep). The deepest coring sites show the best possible record, but one major earthquake is missing and the age of one event deposit does not fit with any known earthquake. More cores are needed to check the spatial extent of each deposit and to validate the absence of record of some earthquakes, like the 1995 Aigion earthquake. [less ▲]

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See detailHeat survival of Clostridium difficile spores in ground meat during cooking process
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Avesani, Véronique et al

Poster (2015, April 21)

Introduction: Clostridium difficile is a spore-forming pathogen considered as a major cause of enteric disease in humans, with faecal-oral route as the primary mode of transmission. However, recent ... [more ▼]

Introduction: Clostridium difficile is a spore-forming pathogen considered as a major cause of enteric disease in humans, with faecal-oral route as the primary mode of transmission. However, recent studies have reported the occurrence of C. difficile in ground meats at retail stores, indicating that foods could be an additional source of infection in the community. Purpose: The objective of this study was to determine the resistance of C. difficile spores in contaminated ground meat during cooking process. Methods: Prior to testing, to obtain spores and to enhance heterogeneity, spores of two different strains were produced in two nutritious broths. C. difficile spores were experimentally inoculated in 45 g of ground meat (beef and pork) in order to obtain a final contamination of 4,500 ufc g-1. Six heating temperatures (70, 75, 80, 85, 90 and 95°C) were chosen. Samples were heating in a water bath with an integrated program for time-temperature. One sample without inoculum was used as control with a temperature probe placed inside. Once the desired temperature was research in the core of the sample, the heat treatment was prolonged for 10 min. Subsequently, all the samples were placed on the chilling room (4°C) before analyse. These experiments were conducted in duplicate with a spore enumeration in triplicate. Results: Heating contaminated ground meat at 70, 75 and 80°C for 10 min was not effective for C. difficile spores inhibition. However, 10 min of heat shock at 80°C was the only temperature that significantly reduced the number of countable colonies. Heat treatment at 85°C (or more) inhibits the germination of both of the strains tested. Significance: Ensure that ground meat, like burgers or sausages, is heated to more than 85°C would be an important measure to reduce the risk of C. difficile food transmission. [less ▲]

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See detailNovel synthetic pyridyl analogues of CDDO-Im with improved stability and their potential use in cancer prevention
Cao, Martine ULg; Onyango, Evans; Williams, Charlotte et al

Poster (2015, April 20)

Synthetic oleanane triterpenoids are non­cytotoxic, multifunctional drugs with a broad spectrum of applications for prevention and treatment of cancer and for many other chronic diseases. CDDO­Im, 1 ... [more ▼]

Synthetic oleanane triterpenoids are non­cytotoxic, multifunctional drugs with a broad spectrum of applications for prevention and treatment of cancer and for many other chronic diseases. CDDO­Im, 1[2­Cyano­3,12­dioxooleana­1,9(11­dien­28­oyl] imidazole, synthesized more than a decade ago, is one of the most potent triterpenoids known to date with marked antiinflammatory, cytoprotective, antiproliferative, differentiative and proapoptotic activity on various human and murine tumor cell lines. However, pharmacokinetics of CDDO­Im are not optimal. Therefore, three new pyridyl analogues of CDDO­Im, namely CDDO­3P­Im, CDDO­2P­Im and CDDO­4P­Im, have been synthesized and screened for their possible use as chemopreventive or chemotherapeutic drugs. At low nanomolar concentrations, they were equivalent to CDDO­Im for induction of differentiation in U937 leukemia cells and at higher doses they induced apoptosis. As inflammation and oxidative stress contribute to carcinogenesis, we also assessed their cytoprotective potential. The new compounds suppressed inducible nitric oxide synthase expression in RAW264.7 macrophage­like cells and significantly induced heme oxygenase­1 and NADPH quinone reductase mRNA and protein levels in lung cancer cells as well as in various mouse tissues. Most importantly, pharmacokinetic studies performed in vitro in human plasma and in vivo revealed superior stability for each new analogue. While CDDO­Im was almost completely degraded after 30 min (< 12 % of starting material remaining) in human plasma, the new compounds were more stable with > 50 % still detectable. Six hours after gavage, much higher concentrations of the new derivatives were found in mouse liver, lung, pancreas and kidney in contrast to CDDO­Im. Thus, the new pyridyl analogues have better bioavailability, and because of their potent anti­inflammatory activity and improved stability, they will be tested in vivo in relevant carcinogenesis models [less ▲]

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See detailGlobal, diffuse and direct irradiances modelling over northwestern Europe using regional climate model MAR : validation and construction of a 30-year climatology
Beaumet, Julien ULg; Doutreloup, Sébastien ULg; Fettweis, Xavier ULg et al

Poster (2015, April 17)

Incoming solar global irradiances are modelled using MAR regional climate model forced by ERA-Interim reanalysis. Global irradiances are decomposed into direct and diffuse using sigmoid model from Ruiz ... [more ▼]

Incoming solar global irradiances are modelled using MAR regional climate model forced by ERA-Interim reanalysis. Global irradiances are decomposed into direct and diffuse using sigmoid model from Ruiz-Arias et al. (2010). Results are validated using data from the European Solar Radiation Atlas for Uccle and Braunschweig weather stations. A 30-year climatology has been built and trends and variability have been analyzed. [less ▲]

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See detailLiposomes entrapping apigenin for the treatment of glioblastoma
Karim, Reatul ULg; Palazzo, Claudio ULg; Dubois, Nadège ULg et al

Poster (2015, April 17)

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See detailDiurnal and seasonal variability of CO2 fluxes over a degraded Woodland under a Sudanian climate in Northern Benin, West Africa
Ago, Expédit Evariste ULg; Serça, Dominique; Agbossou, Euloge Kossi et al

Poster (2015, April 17)

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See detailCorrelation between levels of β-hydroxybutyrate and fatty acids in blood and milk and its impact on ketosis diagnosis in dairy cows
Lessire, Françoise ULg; Knapp, Emilie ULg; Dotreppe, Olivier et al

Poster (2015, April 16)

SKC at herd level is difficult to diagnose. Poor production and reproduction performances are usually observed as an increased incidence of periparturient diseases in the herd (Suthar et al., 2013 ... [more ▼]

SKC at herd level is difficult to diagnose. Poor production and reproduction performances are usually observed as an increased incidence of periparturient diseases in the herd (Suthar et al., 2013). Diagnosis methods include determination of beta-hydroxybutyrate (BHB) and increased non-esterified fatty acids (NEFA) resulting from body fat mobilization. BHB and NEFA could be dosed in blood of animals in late gestation and in early lactation. Post calving, cows presenting BHB over 1. 2-1.4 mmol/L are considered SCK-cows while those presenting NEFA over 0.6 mg/L are labelled fat mobilising cows. Development of non-invasive diagnosis techniques could be interesting to sample animals at a larger scale with lesser stress. The aim of this study was to verify whether blood and milk BHB values were correlated and whether diagnostic methods by milk analysis could be developed. Seventy -five cows out of 8 selected Walloon dairy herds were followed up monthly from calving to pregnancy diagnosis regarding production and reproduction. At each visit (V), BHB and NEFA levels were determined in milk and blood. A maximum of 5 V was made. BHB was determined in blood using a cow-side test and in milk by a colorimetric test . Blood NEFA and milk fatty acids were determined by gas chromatography (GC). Statistical analysis was performed by SAS 9.1. BHB levels in blood and milk were highly correlated (r= 0.86), indicating the possibility of diagnosis of SCK by milk sampling. The earliest the samples have been taken, the better the correlation is (r=0.95 V1; r = 0.91 V2). Comparison of BHB with NEFA demonstrated a better correlation with milk BHB than with blood BHB (respectively 0.51 and 0.53 in milk vs 0.41 and 0.48 in blood for the V1 and V2 respectively). After the 2d V, the correlation dropped to 0.38 (V3) and -0.14 (V4).To conclude, dosage of milk BHB could be a good indicator for ketosis diagnosis taking into account that correlation with blood BHB and with NEFA is time-related. [less ▲]

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See detailGenetic correlations between methane production and milk fatty acid contents of Walloon Holstein cattle throughout the lactation
Vanrobays, Marie-Laure ULg; Vandenplas, Jérémie ULg; Bastin, Catherine ULg et al

Poster (2015, April 16)

Methane (CH4) from ruminal fermentation is the major greenhouse gas produced by dairy cattle which contributes largely to climate change. Production of CH4 also represents losses of gross energy intake ... [more ▼]

Methane (CH4) from ruminal fermentation is the major greenhouse gas produced by dairy cattle which contributes largely to climate change. Production of CH4 also represents losses of gross energy intake. Therefore, there is a growing interest in mitigating these emissions. Acetate and butyrate have common bio-chemical pathways with CH4. Because some milk fatty acids (FA) arise from acetate and butyrate, milk FA are often considered as potential predictors of CH4. However, relationships between these traits remain unclear. Moreover, the evolution of the phenotypic and genetic correlations of CH4 and milk FA across days in milk (DIM) has not been evaluated. The main goal of this study was to estimate genetic correlations between CH4 and milk FA contents throughout the lactation. Calibration equations predicting daily CH4 production (g/d) and milk FA contents (g/100 dL of milk) from milk mid-infrared (MIR) spectra were applied on MIR spectra related to Walloon milk recording. Data included 243,260 test-day records (between 5 and 365 DIM) from 33,850 first-parity Holstein cows collected in 630 herds. Pedigree included 109,975 animals. Bivariate (i.e., CH4 production and one of the FA traits) random regression test-day models were used to estimate genetic parameters of CH4 production and 7 groups of FA contents in milk. Saturated (SFA), short-chain (SCFA), and medium-chain FA (MCFA) showed positive averaged daily genetic correlations with CH4 production (from 0.25 to 0.29). Throughout the lactation, genetic correlations between SCFA and CH4 were low in the beginning of the lactation (0.11 at 5 DIM) and higher at the end of the lactation (0.54 at 365 DIM). Regarding SFA and MCFA, genetic correlations between these groups of FA and CH4 were more stable during the lactation with a slight increase (from 0.23 to 0.31 for SFA and from 0.23 to 0.29 for MCFA, at 5 and 365 DIM respectively). Furthermore, averaged daily genetic correlations between CH4 production and monounsaturated (MUFA), polyunsaturated (PUFA), unsaturated (UFA), and long-chain FA (LCFA) were low (from 0.00 to 0.15). However, these genetic correlations varied across DIM. Genetic correlations between CH4 and MUFA, PUFA, UFA, and LCFA were negative in early lactation (from -0.24 to -0.34 at 5 DIM) and increased afterward to become positive from 15 weeks till the end of the lactation (from 0.14 to 0.25 at 365 DIM). Finally, these results indicate that genetic and, therefore, phenotypic correlations between CH4 production and milk FA vary following lactation stage of the cow, a fact still often ignored when trying to predict CH4 production from FA composition. [less ▲]

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See detailEvaluation of soil structural changes through macroscopic and microscopic measurement
Parvin, Nargish ULg; Degré, Aurore ULg; Chelin, Marie ULg et al

Poster (2015, April 16)

The heterogeneity of soil structure and pore size distribution are highly influenced by external factors like tillage systems and other agricultural management practices. However, changes in soil ... [more ▼]

The heterogeneity of soil structure and pore size distribution are highly influenced by external factors like tillage systems and other agricultural management practices. However, changes in soil hydrodynamic behavior are not fully understood and are still under research. Also, researchers have explained the impact of tillage practices on soil hydraulic properties related to pore size distribution, connectivity and orientation are involved but the characterization of these modifications and consequences remains a challenge. Furthermore, the relation between macroscopic measurements and microscopic investigation of the soil structure remains scarce. Recently, X-ray tomography (X- μCT) has been used in order to characterize changes in soil pore size distribution in various contexts and the method is able to link microtomography information to hydrodynamic measurement. In our study, X-μCT has been used in order to characterize changes in soil pore system. Since, tomography does not count most of the micropores, Richards’ pressure plate and evaporation method was also combined to get complete range of pore size distribution. We found good match between evaporation data with X-μCT at the macropore scale and evaporation data with pressure plate method at micropore scale. X-μCT data refines retention and hydraulic curves near saturation where Richards’ data alone can lead to numerous sets of fitted parameters. On the otherhand, evaporation data (Hyprop apparatus ©) provide comparable datasets with X-μCT. Combining micro and macroscopic measurements allows us to validate X-μCT information, which is otherwise not so obvious. [less ▲]

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See detailVariogram-based inversion of time-lapse electrical resistivity data: development and application to a thermal tracing experiment
Hermans, Thomas ULg; Nguyen, Frédéric ULg

Poster (2015, April 15)

Electrical resistivity tomography (ERT) has become a popular imaging methodology in a broad range of applications given its large sensitivity to subsurface parameters and its relative simplicity to ... [more ▼]

Electrical resistivity tomography (ERT) has become a popular imaging methodology in a broad range of applications given its large sensitivity to subsurface parameters and its relative simplicity to implement. More particularly, time-lapse ERT is now increasingly used for monitoring purposes in many contexts such as water content, permafrost, landslide, seawater intrusion, solute transport or heat transport experiments. Specific inversion schemes have been developed for time-lapse data sets. However, in contrast with static inversions for which many techniques including geostatistical, minimum support or structural inversion are commonly applied, most of the methodologies for time-lapse inversion still rely on non-physically based spatial and/or temporal smoothing of the parameters or parameter changes. In this work, we propose a time-lapse ERT inversion scheme based on the difference inversion scheme. We replace the standard smoothness-constraint regularization operator by the parameter change covariance matrix. This operator takes into account the correlation between changes in resistivity at different locations through a variogram computed using independent data (e.g., electromagnetic logs). It may vary for subsequent time-steps if the correlation length is time-dependent. The methodology is first validated and compared to the standard smoothness-constraint inversion using a synthetic benchmark simulating the injection of a conductive tracer into a homogeneous aquifer inducing changes in resistivity values of known correlation length. We analyze the influence of the assumed correlation length on inversion results. Globally, the method yields better results than the traditional smoothness constraint inversion. Even if a wrong correlation length is assumed, the method performs as well as the smoothness constraint since the regularization operator balances the weight given to the model constraint functional in the objective function. Then the methodology is successfully applied to a heat injection and pumping experiment in an alluvial aquifer. The comparison with direct measurements in boreholes (temperature loggers and distributed temperature sensing optic fibres) shows that ERT-derived temperatures and breakthrough curves image reliably the heat plume through time (increasing part of the curve, maximum and tail are correctly retrieved) and space (lateral variations of temperature are observed) with less spatial smoothing than standard methods. The development of new regularization operators for time-lapse inversion of ERT data is necessary given the broad range of applications where ERT monitoring is used. In many studies, independent data are available to derive geostatistical parameters that can be subsequently used to regularize geophysical inversions. In the future, the integration of spatio-temporal variograms into existing 4D inversion schemes should further improve ERT time-lapse imaging. [less ▲]

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See detailExperimental design to monitor the influence of crop residue management on the dynamics of soil water content
Chelin, Marie ULg; Parvin, Nargish ULg; Hiel, Marie-Pierre ULg et al

Poster (2015, April 15)

Choices related to crop residue management affecting soil structure determine spatio-temporal dynamics of water content and eventually crop yields. In this contribution, we discuss the experimental design ... [more ▼]

Choices related to crop residue management affecting soil structure determine spatio-temporal dynamics of water content and eventually crop yields. In this contribution, we discuss the experimental design we adopted to study the influence of three different agricultural management strategies (tillage and residue management) on the soil water dynamics under maize in a Cutanic Siltic Luvisol in Gembloux, Belgium. In order to limit soil disturbance, we opted for the use electrical resistivity tomography (ERT) and we use the bulk electrical conductivity as a proxy for soil moisture content. ERT is collected every week on a surface of two square meters corresponding to three rows of seven maize plants through surface stainless steel electrodes. Four additional sticks with stainless steel electrodes will be vertically inserted into the soil up to 1.20 m to get more detailed information near to the central maize row. In each of the monitoring plots, two time-domain reflectometry (TDR) probes will be installed for data validation. In order to calibrate the relationship between electrical resistivity and soil water content under highly variable field conditions (changes in soil structure, variable weather conditions, plant growth, fertilization), a trench will be dug, in which a set of four electrodes, one TDR probe and one temperature sensor will be placed at four different depths. In addition, two suction cups will be installed in each of the plots to quantify changes in ion composition and electrical conductivity of the soil solution at two different depths. Within the framework of the multidisciplinary research platform AgricultureIsLife, regular assessment of pore structure and crop developement will be conducted using X-ray images. Combining this wide range of data, we will be able to investigate and quantify the effect of simultaneously changing pore water conductivity, soil porosity, soil temperature and soil moisture on the effectiveness of time-lapse ER measurements as a proxy for soil moisture changes. [less ▲]

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See detailRegularized focusing inversion of time-lapse electrical resistivity data: an approach to parametrize the minimum gradient support functional
Nguyen, Frédéric ULg; Hermans, Thomas ULg

Poster (2015, April 15)

Inversion of time-lapse resistivity data allows obtaining ‘snapshots’ of changes occurring in monitored systems for applications such as aquifer storage, geothermal heat exchange, site remediation or ... [more ▼]

Inversion of time-lapse resistivity data allows obtaining ‘snapshots’ of changes occurring in monitored systems for applications such as aquifer storage, geothermal heat exchange, site remediation or tracer tests. Based on these snapshots, one can infer qualitative information on the location and morphology of changes occurring in the subsurface but also quantitative estimates on the degree of changes in certain property such as temperature or total dissolved solid content. Analysis of these changes can provide direct insight into flow and transport and associated processes and controlling parameters. However, the reliability of the analysis is dependent on survey geometry, measurement schemes, data error, and regularization. Survey design parameters may be optimized prior to the monitoring survey. Regularization, on the other hand, may be chosen depending on available information collected during the monitoring. Common approaches consider smoothing model changes both in space and time but it is often needed to obtain a sharp temporal anomaly, for example in fractured aquifers. We here propose to use the alternative regularization approach based on minimum gradient support (MGS) (Zhdanov, 2002) for time-lapse surveys which will focus the changes in tomograms snapshots. MGS will limit the occurrences of changes in electrical resistivity but will also restrict the variations of these changes inside the different zones. A common difficulty encountered by practitioners in this type of regularization is the choice of an additional parameter, the so-called , required to define the MGS functional. To the best of our knowledge, there is no commonly accepted or standard methodology to optimize the MGS parameter . The inversion algorithm used in this study is CRTomo (Kemna 2000). It uses a Gauss-Newton scheme to iteratively minimize an objective function which consists of a data misfit functional and a model constraint functional. A univariate line search is performed at each Gauss-Newton iteration step to find the optimum value of the regularization parameter  which minimizes the data misfit as a function of  while the data misfit is above the desired value and yields the desired target misfit (root-mean square value of error-weighted data misfit equal to 1) at the last iteration for a maximum value of . We propose here to optimize the  of the MGS functional by considering a univariate line search at the first iteration to find the  that minimizes the data misfit. The parameter is then kept constant during the Gauss-Newton iterative scheme. In this contribution, we validate our approach on a numerical benchmark and apply it successfully on a case study in the context of salt tracers in fractured aquifers. Zhdanov M.S. 2002. Geophysical Inverse Theory and Regularization Problems. Elsevier, Amsterdam, 628 p. Kemna A. 2000. Tomographic Inversion of Complex Resistivity - Theory and Application. PhD Thesis, Ruhr University Bochum. [less ▲]

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See detailThe seamod.ro operational stochasting forecasting system of the Black Sea
Vandenbulcke, Luc ULg; Barth, Alexander ULg; Capet, Arthur et al

Poster (2015, April 15)

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See detailLIPOSOME CONTAINING ESTETROL FOR THE TREATMENT OF ISCHEMIA DISEASES IN PREMATURE BABIES
Palazzo, Claudio ULg; Karim, Reatul ULg; Mawet, Marie et al

Poster (2015, April 14)

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See detailFuture projections of the Greenland ice sheet mass balance using the regional climate MAR model coupled with the GRISLI ice sheet model
Wyard, Coraline ULg; Fettweis, Xavier ULg; Ritz, Catherine

Poster (2015, April 14)

During the two last decades, the Greenland ice sheet (GrIS) contribution to the global mean sea level rise has significantly increased. But, difficulties remain to assess GrIS future contribution because ... [more ▼]

During the two last decades, the Greenland ice sheet (GrIS) contribution to the global mean sea level rise has significantly increased. But, difficulties remain to assess GrIS future contribution because of large uncertainties linked to the feedback between the surface mass balance (SMB) and GrIS topography changes. The regional climate MAR model has been coupled with the GRISLI ice sheet model, in order to account of this feedback in the future projections. The aim of this study is to assess the pertinence of the MAR-GRISLI coupling which requires long computation time. In order to identify GRISLI sensitivity to MAR forcing, GRISLI has been forced with various non-coupled (i.e. using a fixed topography), coupled and modified non-coupled MAR outputs. To adapt the non-coupled MAR outputs to the GRISLI topography changes, we use an interpolation technique based on SMB vs elevation vertical gradient. These experiences evaluate the performances/limits of this interpolation technique used to avoid a RCM-ice sheet model coupling. [less ▲]

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See detailOptical Study of a Spectrum Splitting Solar Concentrator based on a Combination of a Diffraction Grating and a Fresnel Lens
Michel, Céline ULg; Loicq, Jerôme ULg; Thibert, Tanguy ULg et al

Poster (2015, April 14)

This poster presents recent improvements of our new solar concentrator design for space application. The concentrator is based on a combination of a diffraction grating (blazed or lamellar) coupled with a ... [more ▼]

This poster presents recent improvements of our new solar concentrator design for space application. The concentrator is based on a combination of a diffraction grating (blazed or lamellar) coupled with a Fresnel lens. Thanks to this diffractive/refractive combination, this optical element splits spatially and spectrally the light and focus approximately respectively visible light and IR light onto electrically independent specific cells. It avoid the use of MJs cells and then also their limitations like current matching and lattice matching conditions, leading theoretically to a more tolerant system. The concept is reminded, with recent optimizations, ideal and more realistic results, and the description of an experimental realization highlighting the feasibility of the concept, and the closeness of theoretical and experimental results. [less ▲]

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See detailUse of NIR Hyperspectral Imaging and dichotomist classification tree based on SVM in order to discriminate roots and crop residues of winter wheat
Eylenbosch, Damien ULg; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2015, April 14)

NIR Hyperspectral Imaging coupled with SVM chemometric tool is proposed as an alternative method to the tedious and time-consuming hand sorting step needed before root quantification using the soil coring ... [more ▼]

NIR Hyperspectral Imaging coupled with SVM chemometric tool is proposed as an alternative method to the tedious and time-consuming hand sorting step needed before root quantification using the soil coring method. This method was applied to quantify roots under a winter wheat crop. [less ▲]

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See detailSoil porosity in agricultural context: A review of measurement techniques at various scales
Garré, Sarah ULg; Chelin, Marie ULg; Luong, Jeanne ULg et al

Poster (2015, April 13)

Soil compaction was identified by European Commission as one of the eight main threats for agricultural soils. In order to address this issue, measurements of soil porosity are critical. However, there ... [more ▼]

Soil compaction was identified by European Commission as one of the eight main threats for agricultural soils. In order to address this issue, measurements of soil porosity are critical. However, there are as many techniques to measure as there are definitions of porosity. A single method is not sufficient to obtain a complete image of the soil porosity at various scales and encompassing different levels of complexity. Each existing method is characterized by a unique combination of a specific level of complexity, resolution and scale of measurement. In this review, we started by defining the basic terms linked to soil porosity in an agricultural context. Then we give an overview of relevant measurement techniques, from classical methods to recent advances. We present their advantages and disadvantages, the scales of measurement, the resolution, the expected accuracy and the susceptibility to errors. This work aims at guiding the choice for the best (combination of) technique(s) to answer questions related to agricultural soil porosity, categorizing techniques according to the parameters they focus on: from total porosity over pore size distribution, structure and connectivity up to the quantification of spatio-temporal dynamics. [less ▲]

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See detailPreliminary results for a hydrogen maser cavity in the TE111 mode
Van Der Beken, Emeline ULg; Léonard, Daniel; Counet, Arnaud et al

Poster (2015, April 13)

An analysis of a hydrogen maser working with an unusual TE111 mode is presented. Different simulations have been carried out and are compared with preliminary results obtained for such a maser. In ... [more ▼]

An analysis of a hydrogen maser working with an unusual TE111 mode is presented. Different simulations have been carried out and are compared with preliminary results obtained for such a maser. In contrast to standard hydrogen maser that exploits the TE011 mode, the TE111 mode allows one to design hydrogen masers with significant reduced dimensions which represents a huge benefit for space applications and in particular for the global positioning system. [less ▲]

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See detailOrganic and inorganic carbon fluxes in a tropical river system (Tana River, Kenya) during contrasting wet seasons
Geeraert, N; Omengo, FO; Bouillon, S et al

Poster (2015, April 12)

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See detailStone tool hafting and use in the European Upper Palaeolithic: first results from Hohle Fels
Taipale, Noora ULg; Rots, Veerle ULg

Poster (2015, April 07)

European Upper Palaeolithic lithic assemblages have been so far defined largely on a typological or technological basis, whereas extensive studies that would utilise the full potential of functional ... [more ▼]

European Upper Palaeolithic lithic assemblages have been so far defined largely on a typological or technological basis, whereas extensive studies that would utilise the full potential of functional analysis have been few. In this poster, I will present the outline and first results of an ongoing PhD project dedicated to the variability in stone tool use and hafting in the Upper Palaeolithic of Central and Western Europe. Recent methodological developments have made possible the distinction between hafted and hand-held tools and the identification of different hafting modes in archaeological assemblages. The aim of my research is to understand the main developments and regional patterns in tool hafting and use in the Gravettian and Magdalenian, and evaluate their impact on lithic assemblage variability. The cave site Hohle Fels serves here as a case study, and the first results of the analysis of the site’s Gravettian material will be used to illustrate the potential of this kind of approach in the study of past human behaviour, cognition and culture. [less ▲]

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See detailMultitasking abilities in persons diagnosed with schizophrenia: a new tool and cognitive model.
Laloyaux, Julien ULg; Van der Linden, Martial ULg; Laroi, Frank ULg

Poster (2015, April 01)

Difficulties in everyday life activities are core features of persons diagnosed with schizophrenia, and in particular during multitasking activities (Semkovska et al., 2010). Multitasking refers to ... [more ▼]

Difficulties in everyday life activities are core features of persons diagnosed with schizophrenia, and in particular during multitasking activities (Semkovska et al., 2010). Multitasking refers to activities (e.g. preparing a meal) where the person has to: (a) carry out and alternate between different tasks that vary in terms of priority, difficulty and duration; (b) define the tasks’ targets; (c) and where the person is faced with unexpected problems during the realization of these tasks (Burgess, 2000). However, the cognitive underpinnings of multitasking abilities have never been adequately explored in schizophrenia. Further, only two cognitive models exist in the literature, which are based on student (Logie et al., 2011) and neurological (Burgess et al., 2000) samples. Both of these models suggest three primary constructs: Memory, Planning and Intent. There are, however, several limitations related to the way multitasking abilities were evaluated in these studies. We thus developed a computerized real-life activity task - the Computerized Meeting Preparation Task (CPMT), which was specifically designed to take into account the multitasking nature of certain everyday life activities. Using this task, and based on previous studies (Burgess et al., 2000; Logie et al., 2011), the aim of the present study was to evaluate multitasking abilities in schizophrenia and to do so in a new cognitive model of multitasking that takes into account certain cognitive functions that are not integrated in existing models. Methods: Fifty-seven individuals with schizophrenia and 41 matched healthy controls completed the CMPT. Participants were also evaluated with a battery of cognitive tests. Results: The results suggest that the CMPT possesses good sensitivity and confirmed the three underlying constructs of multitasking (Memory, Planning and Intent), which were found to be underpinned by several cognitive functions and multitasking aspects. Conclusion: Taken together, this new cognitive model and the CMPT could be a good basis for cognitive interventions of multitasking abilities in schizophrenia. Burgess, P.W., 2000. Strategy application disorder: the role of the frontal lobes in human multitasking. Psychol Res 63, 279-288. Logie, R., et al., 2011. Multitasking: multiple, domain-specific cognitive functions in a virtual environment. Mem Cogn 39, 1561-1574. Semkovska, M. et al., 2004. Assessment of executive dysfunction during activities of daily living in schizophrenia. Schizophr Res 69, 289-300. [less ▲]

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See detailAssociation of classical microbiology and 16S rDNA metagenetic analysis to evaluate the presence of Clostridium difficile ina a belgian nursing home
Rodriguez Diaz, Cristina ULg; Taminiau, Bernard ULg; Avesani, Véronique et al

Poster (2015, April 01)

Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI ... [more ▼]

Increasing age, several co-morbidities, environmental contamination, antibiotic exposure and other intestinal perturbations appear to be the greatest risk factors for C. difficile infection (CDI). Therefore, elderly care home residents are considered particularly vulnerable to CDI. The main objective of this study was to evaluate and follow the prevalence of C. difficile in a Belgian nursing home. During a 4-month period, stool samples from a group of 23 elderly care home residents were collected weekly. A C. difficile microbiological detection scheme was performed along with an overall microbial biodiversity study of the faeces content by Targeted Metagenomic analysis. Culture of samples was performed in a selective medium cycloserine cefoxitin fructose cholate. An identification of the isolated colonies was done by PCR detection of tpi, tcdA, tcdB and cdtA genes. Toxic activity was confirmed by a cytotoxic immunoassay. Further characterization was performed by PCR ribotyping. The Metagenomic analysis was targeted on the v1-v3 hyper-variable region of 16S rDNA. The taxonomical assignment of the populations was performed with MOTHUR and Blast algorithms. Seven out of 23 (30.4%) residents were (at least one week) positive for C. difficile. The most common PCR-ribotype identified was 027. Targeted Metagenomic analyses reveals that each resident has his own bacterial imprint, which is stable during the entire study. Residents’ positives for C. difficile by classical microbiology showed an important proportion of C. difficile sequences. However, Metagenomics analysis can’t substitute targeted protocols. It was not used as a diagnostic tool to detect C. difficile but rather to determine the identification and correlations of the major bacterial populations that are present in the gut microbiota. In conclusion, this unique association of classical microbiology protocol with pyrosequencing allowed to follow C. difficile in patients and to identify several other bacterial populations whose abundance is correlated with C. difficile. [less ▲]

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See detailTesting the impact of stratigraphic uncertainty on spectral analysis of sedimentary time series
Martinez, Mathieu; Kotov, Sergey; De Vleeschouwer, David et al

Poster (2015, April)

Spectral analysis has become a key tool for identifying the imprint of astronomical forcing on sedimentary records. In a next step, the identified cycles often contribute to the construction of a precise ... [more ▼]

Spectral analysis has become a key tool for identifying the imprint of astronomical forcing on sedimentary records. In a next step, the identified cycles often contribute to the construction of a precise Geological Time Scale and to an in-depth understanding of past climate changes. Most of spectral analyses (Fast Fourier Transforms, the Multi-Taper Method. . . ) require a constant sample step. Unfortunately, an equally spaced geological data series is, in practice, nearly impossible to obtain from field sedimentary series. Usually, there is a 10% uncertainty on the field measurements of the stratigraphic thickness within sedimentary series. Hence, important uncertainties exist on the actual position of each sample. Another source of uncertainty are errors in a time-space model. In this study, we explore the impact that the stratigraphic uncertainty on the sample position has on the result of spectral analyses. To simulate this uncertainty, we developed a model based on the Monte Carlo randomisation of the distance between each successive point. In this way, the stratigraphic order of the data points is not affected after imp lementating this model. The application of this model to a theoretical sinusoid series and to several real sedimentary series shows that uncertainties in the actual position of samples can highly reduce the spectral powers of the frequencies ranging from the Nyquist Frequency up to 1/10 of the Nyquist Frequency. We the demonstrate that the precise reconstruction of the Milankovitch cycles in the sedimentary record requires a higher sampling density than previously suggested with, at least, 10 samples per thinnest cycle to be detected, i.e. 10 samples per precession cycle [less ▲]

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See detailInfection expérimentale du porc par une souche du virus de l’hépatite E isolée du sanglier
Thiry, Damien ULg; Rose, Nicolas; Mauroy, Axel ULg et al

Poster (2015, April)

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See detailCombining tree-based and dynamical systems for the inference of gene regulatory networks
Huynh-Thu, Vân Anh ULg; Sanguinetti, Guido

Poster (2015, April)

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See detailDimethylsulfoniopropionate (DMSP) cell quota of key Southern North Sea spring diatoms and Phaeocystis globosa
Speeckaert, Gaëlle ULg; Gypens, Nathalie; Lancelot, Christiane et al

Poster (2015, April)

Dimethylsulfide (DMS) in the ocean results of complex transformations of dimethylsulfoniopropionate (DMSP) produced by phytoplankton under different controls, including microbial transformation pathways ... [more ▼]

Dimethylsulfide (DMS) in the ocean results of complex transformations of dimethylsulfoniopropionate (DMSP) produced by phytoplankton under different controls, including microbial transformation pathways. The phytoplankton composition is an important factor of variability due to the species dependence of the DMSP production and conversion to DMS. To better appraise the link between phytoplankton diversity and the DMS(P) cycling in the Southern North Sea we present measurements of the DMSP cell quota of key spring phytoplankton species (Skeletonema costatum, Thalassiosira rotula, Rhizosolenia delicatula, Asterionella glacialis, Nitzschia closterium, Chaetoceros debilis, Chaetoceros socialis and Phaeocystis globosa) isolated from the North Sea and maintained in non-limiting and axenic laboratory culture conditions. Results are discussed with regards to literature data and hypothesis currently used in DMS(P) biogeochemical models. [less ▲]

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See detailDid the savannah « flourished » 3000 years ago in the so-called Sangha River Interval of the Guineo-Congolian rainforest ? A retrospective study using stable isotopes and phytoliths
Bentaleb, Ilham; Freycon, Vincent; Gillet, Jean-François et al

Poster (2015, April)

We aim to improve our knowledge of the dynamic of the vegetation in Central Africa during the last 5 kyrs and to discuss the main hypothesis described in the literature - humans versus climatic impacts ... [more ▼]

We aim to improve our knowledge of the dynamic of the vegetation in Central Africa during the last 5 kyrs and to discuss the main hypothesis described in the literature - humans versus climatic impacts- both suggested as responsible of the Congo basin rainforest decline observed between 3 and 2.5 kyrs. We use the carbon isotopic composition of well-dated Central African soils to reconstruct the dynamic of the vegetation cover. We will discuss the carbon isotopic composition of the soil organic carbon methodology for reconstructing palaeovegetation in the light of Rayleigh distillation model. We showed that numerous sites exhibit a carbon isotopic ratios reflecting the Rayleigh distillation but few sites recorded real vegetation changes. Our study suggests that the vegetation of the Guineo-Congolian Region was disturbed between 3000 and 2000 BP (Before Present) without an extreme savannah expansion. We discussed the two hypotheses human versus climate impacts that may conduct to such new physiography of the vegetation. We suggest that the climate hypothesis is more likely than the human impact to explain the reduction of the Guineo-Congolian rainforest 3000 years ago. [less ▲]

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See detailSoil microbial community composition changes according to the tillage practice and plant development stage
Degrune, Florine ULg; Dufrêne, Marc ULg; Taminiau, Bernard ULg et al

Poster (2015, April)

Agricultural practices have a strong impact on soil bacterial and fungal community composition. Furthermore, microbial community composition can change with the stage of plant development. We are ... [more ▼]

Agricultural practices have a strong impact on soil bacterial and fungal community composition. Furthermore, microbial community composition can change with the stage of plant development. We are interested in exploring these effects in relation to changes induced by agriculture (conventional and reduced tillage) and plant stage (germination and flowering) in soil conditions. Here, instead of examining this impact at a high taxonomic level such as phylum and/or class, thus missing potentially relevant information from lower levels, we propose an original method: exploiting the available sequence information at the lowest taxonomic level attainable for each operational taxonomic unit. Results show that some microbial communities were impacted only by the tillage practice , while others were impacted only by the stage of plant. Changes in microbial community composition could be due to the soil conditions induced by the soil practice and the stage of plant. [less ▲]

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See detailDecrease of the Black Sea Oxygen Inventory through the second half of the XXth century
capet, arthur; stanev, Emil; Grégoire, Marilaure ULg

Poster (2015, April)

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See detailThe iPot Project: improved potato monitoring in Belgium using remote sensing and crop growth modelling
Piccard, I.; Nackaerts, K.; Gobin, A. et al

Poster (2015, April)

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See detailRemote control of self-assembled magnetocapillary microswimmers
Grosjean, Galien ULg; Lagubeau, Guillaume ULg; Hubert, Maxime ULg et al

Poster (2015, April)

Physics governing the locomotion of microorganisms and other microsystems is dominated by viscous damping. An effective swimming strategy involves the non-reciprocal and periodic deformations of the ... [more ▼]

Physics governing the locomotion of microorganisms and other microsystems is dominated by viscous damping. An effective swimming strategy involves the non-reciprocal and periodic deformations of the considered body. Herein, we show that a magnetocapillary-driven self-assembly, composed of three soft-ferromagnetic beads, is able to swim along a liquid-air interface when driven by an external magnetic field. Moreover, the system can be fully controled, opening ways to explore low Reynolds number swimming and to create micromanipulators in various applications. [less ▲]

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