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See detailFunctional study of Arabidopsis thaliana ASF/SF2-like pre-mRNA SR splicing factors
Stankovic, Nancy ULg; Tillemans, Vinciane; Hanikenne, Marc et al

Poster (2010, January 26)

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See detailExpression of the metal homeostasis gene FRD3 in two Arabidopsis species
Charlier, Jean-Benoit ULg; Polese, Catherine; Motte, Patrick ULg et al

Poster (2010, January 26)

Detailed reference viewed: 32 (11 ULg)
See detailIdentification, characterization and expression profiling of the tomato gene TomLoxF
Mariutto, Martin ULg; Duby, Franceline ULg; Adam, Akram et al

Poster (2010, January 26)

Detailed reference viewed: 33 (10 ULg)
See detailFood and feeding of Limnothrissa miodon and Lamprichthys tanganicanus in Lake Kivu
Masilya, M Pascal; Isumbisho, Mwapu Pascal; Darchambeau, François ULg et al

Poster (2010, January 13)

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See detailModelling total soil respiration in agricultural soils.
Buysse, Pauline ULg; Goffin, Stéphanie; Carnol, Monique ULg et al

Poster (2010, January 12)

Soil respiration is a process which results in CO2 release from the soil to the atmosphere. It comprises two main components. The first one is heterotrophic respiration: CO2 is produced by soil ... [more ▼]

Soil respiration is a process which results in CO2 release from the soil to the atmosphere. It comprises two main components. The first one is heterotrophic respiration: CO2 is produced by soil microorganisms while decomposing the substrate. The second one is autotrophic respiration in which CO2 originates from roots and rhizospheric organisms. All the CO2 is then transported to the surface by diffusion (see Goffin et al., this session). Many biotic and abiotic factors play a role in soil respiration, making this process complex to analyze and understand. Temperature often appears as the most important driving variable. Besides that, interest in the future CO2 emissions from agricultural soils has been growing. Indeed, these ecosystems are a major concern from environmental, economic and social points of view. In particular, the choice of cultural practices and residue management techniques has a strong influence on CO2 emissions from agricultural systems. This work aims at getting to a better understanding of soil respiration in agricultural soils. To reach this goal, many semi-mechanistic models have been previously developed at very different spatio-temporal scales. We intend to adapt such an existing model to crop soils, within a spatial scale of a cultivated field and an annual temporal scale. The model will be validated by using flux measurements carried out at three different crop sites situated in the Hesbaye region in Belgium (Lonzée) and in the South West of France (Lamasquère, Auradé). The study was focused first on soil heterotrophic respiration. Within this part, short term sensitivity of this component to temperature was studied by means of a laboratory incubation experiment. This one was performed with soil samples taken at the Lonzée site. Among the many interesting results we got, it showed a clear sensitivity of soil heterotrophic respiration to short term temperature changes. In parallel, the soil heterotrophic model was calibrated on soil chamber measurements taken at the Lonzée site (Belgium). Next steps in this part of the work will be to calibrate the model using the data from the French sites, and finally to validate the model on the three sites. Afterwards, an autotrophic respiration submodel will be implemented and the results compared to field measurements carried out at the three sites. A further development could consist in simulating agricultural practices to take their impacts on CO2 emissions from crops into account. [less ▲]

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See detailCan an Electronic Nose Assess the Biomethanation Process?
Adam, Gilles ULg; Nicolas, Jacques ULg

Poster (2010, January 12)

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See detailElectrical resistivity tomography and self-potential methods as a tool for fractured aquifer characterization and monitoring
Robert, Tanguy ULg; Nguyen, Frédéric ULg

Poster (2010, January 12)

Electrical resistivity tomography (ERT) and self-potential (SP) investigations are currently conducted in carboniferous limestones in Belgium. The aims of this study are (1) to characterize and (2) to ... [more ▼]

Electrical resistivity tomography (ERT) and self-potential (SP) investigations are currently conducted in carboniferous limestones in Belgium. The aims of this study are (1) to characterize and (2) to monitor the groundwater flow in fractured or karstic area and (3) to use these geophysical data as a help for groundwater flow model conceptualisation and inverse calibration. Large ERT profiles (320 meters) allow us to image the electrical resistivity distribution of the first 60 meters of the subsurface and to detect and characterize (in terms of direction, width and depth) some fractured and/or karstic zones expected to be less resistive. Data errors as well as indicators of resolution (resolution matrix, sensitivity matrix and DOI index) are analysed in order to calculate the depth of investigation of ERT and to avoid the misinterpretation of the resulting images. Self-potential measurements are performed along the electrical profiles and permit us to find some negative anomalies possibly related with groundwater preferential flow pathways. Since SP signals are related with the groundwater flow when taking into account the electrokinetic effect, we are able to estimate a first distribution of the water table along our profiles. These geophysical data concurrently with ‘ground truth’ geological and hydrogeological data give us a way to better understand the groundwater flow in limestone synclines of the Dinant Synclinorium geological structure. Still, further efforts are needed (1) to fully cover the syncline area and (2) to couple the SP, ERT and hydrogeological data through a more complex forward model of the electrokinetic effect of the self-potential method. [less ▲]

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See detailImage Appraisal Tools for Electrical Resistivity Tomography
Caterina, David ULg; Beaujean, Jean ULg; Robert, Tanguy ULg et al

Poster (2010, January 12)

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See detailModelling of Forest Trees Autecology. An applied research at the Walloon Region scale (Belgium)
Ridremont, François ULg; Claessens, Hugues ULg; Lejeune, Philippe ULg et al

Poster (2010, January 12)

Three reasons have urged the Walloon Forest Administration (Walloon Public Service) on to solicit the help of forest researchers to bring practical responses to the adaptation of its current forests ... [more ▼]

Three reasons have urged the Walloon Forest Administration (Walloon Public Service) on to solicit the help of forest researchers to bring practical responses to the adaptation of its current forests management: (i) the progression of research in applied autecology, (ii) the development of software for the management and the analysis of georeferenced data (GIS technologies) and, (iii) the awareness of the potential negative impacts of climate change on forests. To answer to this request, this research aims to model the autecology of the main forest tree species at the Walloon Region scale (Beech, Oak, Norway Spruce, Douglas-fir and valuable broadleaves species). The results will be used to update current practical tools to assess the adequacy of forest tree species on sites, especially in the climate change context. The research is built into three main steps: • update the autecology knowledge for the targeted forest tree species through a state of the art; • compile and create different ecological parameters that can explain the major part of trees growth such as climatic, topographic, geologic, edaphic and phytosociological data gathered from forest plots or geodatabases. In the context of climate change, a particular attention will be brought on bioclimatic and soil moisture conditions because of their narrow link with the potential risk of trees ecophysiological stress; • model tree species reaction to environmental conditions with tree growth indicators. Three types of indicators with different spatio-temporal scales are foreseen: stand site index (30-50 years), periodic diameter growth (5-10 years) and tree-ring growth (1-3 years). The use of these three scales is justified by the interest presented by their cross-validations which are necessary for an accurate extrapolation of results to the entire region. Results should indicate the tree species behaviour, especially towards stress conditions. The integration of climatic data, provided by global climate models based on IPCC's climate scenarios, in autecological models could be used to assess the behaviour of forest stands in future climate conditions. From those results, we expect to identify and assess accurately the risky “sites-species pairs” to avoid in the context of a sustainable silviculture. Finally, results will be used to provide autecological synthesis and practical tools to help forest managers and foresters to identify the best “site-species pairs” (tree-site adequacy maps, sites catalogue and computer platform). [less ▲]

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See detailAnalysis of aldehydes relaesed by rice flour using sorbent adsorption and thermal desorption - gas chromatography/mass spectrometry: Development and validation
Mertens, Cécile ULg; Marlet, Christelle ULg; Blecker, Christophe ULg et al

Poster (2010, January)

Odor is an essential characteristic and a quality marker in baby foodstuff, like a partially hydrolyzed and pregelatinized rice flour. The rice flour study by active sampling and TD-GC/MS highlighted the ... [more ▼]

Odor is an essential characteristic and a quality marker in baby foodstuff, like a partially hydrolyzed and pregelatinized rice flour. The rice flour study by active sampling and TD-GC/MS highlighted the presence of three aldehydes: Hexanal, Octanal, Nonanal. These compounds are known to be caused by lipid oxidation, one of the major alteration reactions in food. The Method was developed and valited on a large range for the three aldehydes. It was then applied to the rice fllour to determine if it can be used to follow the effect of ageing on the aldehydes production. [less ▲]

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See detailHarmonia axyridis (Pallas) secondary metabolites quantification in relation with aposematism : Part I : Harmonine quantification
Fischer, Christophe ULg; Heuskin, Stéphanie ULg; Laurent, Pascal ULg et al

Poster (2010, January)

These last few years, the multicoloured Asian ladybird, Harmonia axyridis Pallas (Insects: Coleoptera: Coccinellidae), an invasive species, has swiftly spread out in Europe. This aposematic and highly ... [more ▼]

These last few years, the multicoloured Asian ladybird, Harmonia axyridis Pallas (Insects: Coleoptera: Coccinellidae), an invasive species, has swiftly spread out in Europe. This aposematic and highly polymorphic insect is problematic in several ways. It seems to outcompete native coccinellid species; they can group on fruits in vineyards and spoil wine when crushed with grapes; they can aggregate by hundreds in houses to winter. This study aims to investigate the relations between the chemical defences of H. axyridis and warning signals expressed by this bug. To achieve this goal, two original quantification methods of H. axyridis secondary metabolites have been undertaken. The first one allows Fast-GC quantifying harmonine, its main defence alkaloid. The other one permits to determine its emission rate of 2-isopropyl-3-methoxypyrazine, a highly odorous compound probably involved in the communication between individuals and in the aposematism of this insect. This last point will be detailed in another poster. Firstly, a method of extraction, acetylation and purification of harmonine from H. axyridis has been devised to obtain a calibration standard because this molecule is not commercially available. The identity of the extracted compound has been confirmed by mass spectrometry and multidimensional NMR. The GC purity of this N,N-diacetylharmonine is of 100%. During the experiments, its stability in acetone solution at 4°C has been verified. Using this purified molecule, an original method of harmonine quantification has been developed by fast gas chromatography and validated using accuracy profile. The method has been validated for concentrations ranging from 28µg/ml to more than 264µg/ml, with a maximal error risk of 20%. From about 100µg/ml, peaks symmetry decreased, but did not influence the quantification. That accuracy profile validation has been compared with a more classical validation using AOAC (2006) norm, which confirmed the first validation. This method allows quantifying harmonine in one single insect. Harmonine has been quantified in 80 H. axyridis divided into four groups of at least 15 individuals on basis of the morph type and the elytra hue. Males and females harmonine contents did not significantly differ. The mean harmonine content was of 106.6µg/individual with a standard deviation of 80.2µg/individual. The results distribution obtained was not normal and a strong variability between individuals was observed, which explains the high standard deviation. These results and those of the pyrazine and colour analysis have been statistically analyzed in order to show potential correlations between these factors. It would appear that elytra colour is linked with the insect harmonine content. Individuals with a non-melanic orange colour presented harmonine content significantly higher than other studied colours. The harmonine content does not seem linked to the pyrazine emission rate. [less ▲]

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See detailLow detection HPLC – DAD analysis of aldehydes and ketones in indoor air : Development and validation by accuracy profile
Marlet, Christelle ULg; Haesen, Charlotte ULg; Lognay, Georges ULg

Poster (2010, January)

Aliphatic aldehydes and ketones like formaldehyde are reactive volatile compounds and common indoor pollutants. The main sources are tobacco smoke, combustion gases, painting and materials. Theses ... [more ▼]

Aliphatic aldehydes and ketones like formaldehyde are reactive volatile compounds and common indoor pollutants. The main sources are tobacco smoke, combustion gases, painting and materials. Theses compounds also arise from the reaction between unsaturated compounds and oxidative agents. The toxic potential of formaldehyde and other aliphatic/aromatic carbonyl compounds requires the determination of even low amounts of these compounds in indoor air. The present method for indoor monitoring uses adsorption at 2,4-dinitrophenylhydrazine (DNPH)-coated sorbents followed by HPLC-UV/DAD analysis of the extract. Detection limits of 0.1μg/m3 were reached by this method which is sufficient for indoor air monitoring. Three DNPH-derivatives could be separated well on a RP-Amide column followed by UV/DAD-detection at 360 nm. Recovery rates of 70-100 % were determined using liquid spiking method. Validation strategy was intentionally oriented towards the ease of use in routine and the liability of the method rather than extreme performances. For that, the validation by accuracy profile was chosen. The accuracy profile, based on the concept of total error (bias + standard deviation), allows to correctly conclude about the validity of a procedure and thus, guarantees that a known proportion of future results obtained with the method will be included within acceptance limits. For the three tested compounds, formaldehyde, acetaldehyde and acetone, the accuracy profile gave the guarantee that at least 95% of the future results obtained with the validated method will be within the ±25% acceptance limits over the whole concentration range defined. The HPLC parameters were also determinated. The performance of the method was finally evaluated by the analysis of indoor air from new timber frame construction. [less ▲]

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See detailHarmonia axyridis (Pallas) secondary metabolites quantification in relation with aposematism : Part II : Pyrazine quantification
Fischer, Christophe ULg; Heuskin, Stéphanie ULg; Marlet, Christelle ULg et al

Poster (2010, January)

The main objective of this study is to investigate the presence of links between chemical defences and warning signals presented by Harmonia axyridis (Pallas), the multicoloured Asian ladybird (Insects ... [more ▼]

The main objective of this study is to investigate the presence of links between chemical defences and warning signals presented by Harmonia axyridis (Pallas), the multicoloured Asian ladybird (Insects: Coleoptera: Coccinellidae). This second part describes an original quantification method for 2-isopropyl-3-methoxypyrazine (IPMP) by active sampling on thermally desorbed adsorbent (TD) coupled with GC-MS analysis. This highly odorous compound is supposed to be implicated in intra- and interspecific communication. In order to allow harmonine quantification (a defence alkaloid, see Part 1), this method avoid any hemolymph loses by the ladybird (Coccinellids can emit hemolymph when stressed). This method is non-lethal and applicable on one single insect. The quantification method has been validated from 37,5pg to 10 000 pg per adsorbent cartridge. On this range, there is no breakthrough and desorption is complete (>99.4%). At the LOQ (37.5pg/cartridge), the bias is of 20%, but rapidly decreased to less than 5% on the rest of the validated range (100 to 10 000 pg/cartridge). According to AOAC (2006) norm, this method is repeatable but not reproducible. The IPMP emission rate following a stress has been quantified on 80 H. axyridis grouped in four classes on basis of the morph type and the elytra hue. Males and females emission rates did not significantly differ. The mean emission rate is of 707 pg/4h with an extremely high standard deviation of 1766pg/4h due to a great individual variability and a strongly asymmetric distribution of the results. These results and those of the harmonine and colour analysis have been statistically analyzed in order to show potential correlations between these factors. It seems that elytra colour is linked with the insect IPMP emission rate. Individuals with a melanic red colour presented IPMP emission rate significantly lower than other studied colours. The harmonine content does not seem linked to the pyrazine emission rate. [less ▲]

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See detailADDICTION AU MMORPG : QUAND LE REEL EST SOUS L’EMPRISE DU VIRTUEL
Bataille, Stéphanie; Triffaux, Jean-Marc ULg

Poster (2010, January)

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See detailA fast gas chromatographic method for the study of semiochemical slow release formulations
Heuskin, Stéphanie ULg; Lorge, Stéphanie; Leroy, Pascal ULg et al

Poster (2010, January)

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See detailIDENTIFICATION OF METABOLITES IN A HUMAN PLASMA STANDARD REFERENCE MATERIAL BY MULTIPLE GC/MS APPROACHES
Eppe, Gauthier ULg; Benner, Bruce; White, Edward et al

Poster (2010, January)

Detailed reference viewed: 27 (1 ULg)
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See detailVolumetric Oxygen Transfer Coefficient and Foaming in Inverse Fluidised Bed Recycle reactor for biosurfactants production
Nikov; Fahim, S.; Dimitrov, K. et al

Poster (2010)

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See detailUnit stream power as a base for regional river typology
Hallot, Eric ULg; Houbrechts, Geoffrey ULg; Levecq, Yannick et al

Poster (2010)

In order to assess the hydromorphological quality of surface waters, stakeholders need river typologies accurate and functional enough to be a really efficient management tool. From a typological study ... [more ▼]

In order to assess the hydromorphological quality of surface waters, stakeholders need river typologies accurate and functional enough to be a really efficient management tool. From a typological study based a broad set of hydromorphological field data, we have shown that some variables, like the unit stream power (), are particularly appropriate to describe the dynamics of a river. Initial results of repeated measurement in more than 80 sites revealed a regional differentiation based on critical values of stream power at bankfull discharge. The aim of this study is to present a unit stream power mapping for the whole network based on general equation  =  g Q S / w, with  the density of water, g the acceleration due to gravity, Q the discharge, S the slope and w the channel width). Slope is extracted from DEM. Bankfull discharge and width estimations are furnished by allometric equations using the watershed area. Different coefficients are used according to regional differentiations based on bedload size and watershed permeability. The statistical significance of all equations was tested by covariance analysis. A correction taking into account the used log-log scale, was also applied to the obtained allometric equations (Fergusson, 1988). Using a 30 x 30 meters DEM, GIS routines were developed. A first one automatically sectorizes the network and a second evaluates the unit streamspower in each location. We propose a 7-level classification in accordance with the associated morphological processes describe in the literature (from fixed meanders to step-pools system). The mapping unit starts at the Strahler second order. Our results not only support a regional typology, but moreover allow us to describe natural regions in function of river dynamics and to estimate the resistance of restoration works like vegetal techniques. It can also provide estimates of the excess stream power (the fraction of the actual stream power exceeding the power at which bedload particles start to move). (Fergusson, R.,I., 1988. River loads underestimated by rating curves, Water Resour. Res., 24(7), 1217–1219.) [less ▲]

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See detailComparison of two drainage flow situations on a gentle forested slope
Heinesch, Bernard ULg; Yernaux, Michel ULg; Aubinet, Marc ULg

Poster (2010)

Drainage flows generated on a gentle slope in stable conditions were analysed at the forested site of Vielsalm (Belgium). Two distinct situations were encountered, one corresponding to vertical ... [more ▼]

Drainage flows generated on a gentle slope in stable conditions were analysed at the forested site of Vielsalm (Belgium). Two distinct situations were encountered, one corresponding to vertical convergence, characterised by a negative vertical velocity at the canopy top, the other corresponding to an equilibrium situation without any vertical movement. The causes of these two distinct flow patterns were analysed. Moreover, combined measurements of vertical velocity above the canopy and horizontal velocity divergence below the canopy were found to comply with the continuity equation: an acceleration of the horizontal flow was indeed observed in the trunk-space in convergence situations while not in equilibrium conditions. These measurements combined with those of the horizontal [CO2] gradient below the canopy were found to met the dilution hypothesis suggested by Aubinet et al.(2003): the horizontal [CO2] gradient was negative in convergence situations while slightly positive in equilibrium conditions. The existence of such patterns allows us to confirm the coherence of advection observations made at the site and help to better understand the mechanisms responsible of night flux exchanges at work in stable conditions in complex terrains. Nevertheless, difficulties were met when trying to obtain reliable estimates of the advection transport terms. The estimation of the vertical velocity in the trunk-space obtained by using the divergence measurements refuted the hypothesis of linearity of the vertical profile of vertical velocity, assumption usually used when computing vertical advection. This problem resulted in a great uncertainty on vertical advection which did not allowed to improve the night-flux estimation by adding advection terms to the turbulent flux and the storage. [less ▲]

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See detailCharacterization of the antimicrobial activity of mycosubtilin on the plasma membranes. A biomimetic approach
Nasir, Mehmet Nail ULg; Besson, Françoise

Poster (2010)

Mycosubtilin, an antimicrobial lipopeptide, is produced by Bacillus subtilis strains. It belongs to the iturin family, which is characterized by the presence of a peptide part, constituted of a constant ... [more ▼]

Mycosubtilin, an antimicrobial lipopeptide, is produced by Bacillus subtilis strains. It belongs to the iturin family, which is characterized by the presence of a peptide part, constituted of a constant chiral amino acid sequence cycled by a β-amino fatty acid (Fig. 1). As all the iturinic lipopeptides, mycosubtilin exhibits its biocide activities on cytoplasmic membrane of target cells [1]. Recently, the activity of mycosubtilin on pathogenic strains resistant to classical agents was shown [2]. However, despite many works focused on its structure and the optimization of its production, only a few studies are conducted to analyze mycosubtilin-membrane interactions. The purpose of our work was to better understand, at the molecular level, the mechanisms of the mycosubtilin activity on cytoplasmic membranes. Firstly, we modelled the mycosubtilin-membrane interactions by using biomimetic monolayers and their associated techniques (tensiometry and PM-IRRAS). After characterizing the interfacial properties of pure mycosubtilin [3], we used Langmuir films to investigate the mycosubtilin behavior when the lipopeptide reaches the external leaflet of the membrane. We found that the mycosubtilin adsorption to lipid monolayers depended on their lipid composition and the lipopeptide interaction with the membrane was facilitated by the presence of sterols. Then, we mimicked the insertion of the lipopeptide in the whole membrane by using multilamellar vesicles. FT-IR spectroscopy analysis showed that the interaction of mycosubtilin with the artificial membranes induced conformational changes of the lipopeptide only in the presence of sterol. 1. Maget-Dana R, Peypoux F. (1994) Toxicology 87:151-74. 2. Fickers P, Guez JS, Damblon C, Leclère V, Béchet M, Jacques P, Joris B. (2009) Appl. Environ. Microbiol. 75:4636-40. 3. Nasir MN, Thawani A, Kouzayha A, Besson F. (2010) Colloids Surf. B Biointerfaces 78 :17-23. [less ▲]

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See detailMutliproxy investigation of climatic and anthropogenic changes in a Baltic bog (N. Poland) during the last millennium
De Vleeschouwer, François; Fagel, Nathalie ULg; Piotrowska, Natalia et al

Poster (2010)

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See detailStudy of mechanical, physical and chemical properties of a-C:H films deposited by reactive magnetron sputtering in DC pulsed mode
Colaux, J. L.; Dumont, D.; Delwigne, T. et al

Poster (2010)

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See detailSURVEY ON ANTIBIOTIC USE IN PIG AND CHICKEN PRODUCTION IN RED RIVER DELTA OF VIETNAM.
Pham Kim, Dang; Saegerman, Claude ULg; Vu Dinh, Ton et al

Poster (2010)

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See detailModeling of the longitudinal modulation of the Io interaction
Hess, S.; Bonfond, Bertrand ULg; Delamere, P. A. et al

Poster (2010)

The Io interaction with the Jovian magnetosphere is the best known case of moon-magnetosphere interaction. It leads to bright emissions from radio to UV, which are mostly due to the acceleration of ... [more ▼]

The Io interaction with the Jovian magnetosphere is the best known case of moon-magnetosphere interaction. It leads to bright emissions from radio to UV, which are mostly due to the acceleration of electrons by Alfvén waves along the magnetic field lines passing Io. We previously presented a model of the electron acceleration by the Iogenic Alfvén waves, which explains the average brightness of the emissions. We now present a study of the modulation of the Io-magnetosphere interaction with longitude, and compare our results with the observed brightness modulation of the auroral emissions. This study highlights how the satellite-magnetosphere interactions depend on the variation of the magnetic field, the equatorial plasma density,... A new model of the Jovian internal magnetic field, giving an accurate description of the longitudinal modulations of the Jovian magnetic field has been computed for this study, and is also presented. [less ▲]

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See detailHeralded entanglement of arbitrary degree in remote atoms by detection of emitted photons
Schilling, U.; Thiel, C.; Solano, E. et al

Poster (2010)

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See detailThe Venus OH Nightglow Distribution
Soret, Lauriane; Gérard, Jean-Claude ULg; Piccioni, G. et al

Poster (2010)

The study shows that the OH emission is highly variable, ranging from less than 20 kR to about 2 MR. The peak brightness appears to decrease away from the antisolar point even if the variability at a ... [more ▼]

The study shows that the OH emission is highly variable, ranging from less than 20 kR to about 2 MR. The peak brightness appears to decrease away from the antisolar point even if the variability at a given location is very strong. By contrast, although the OH peak altitude also appears to be variable, it shows no dependence with the location on the nightside of Venus. Some correlation between simultaneous observations of the intensity of the OH and the O2(a1Δ) emissions has also been detected, presumably because atomic oxygen is a common precursor to the formation of O2(a1Δ) and O3, whose reaction with H produces excited OH. Anyway, it is important to keep in mind that horizontal transport plays an important role in the redistribution of photochemically produced species such as O, O3 and minor long-lived species and possibly explains some of the variability of the OH emission and its brightness relative to O2(a1 ). [less ▲]

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See detailThe HelpED Study: Degree of Agreement Between Patients, Partners, and Physicians on
Claes, Hubert; Andrianne, Robert ULg; Opsomer, Reinier

Poster (2010)

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See detailhCG: a pregnancy-related hormone stimulating angiogenesis and pericyte recruitment
Berndt, S; Blacher, Silvia ULg; Perrier d’Hauterive, S et al

Poster (2010)

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See detailThe HelpED Study: Impact of Change in Erection Hardness on the Self-Esteem,
Opsomer, Reinier; Claes, Hubert; Andrianne, Robert ULg

Poster (2010)

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See detailStatistical approach of monomer diversity in nonribosomal peptides
Caboche, S.; Leclere, V.; Pupin, M. et al

Poster (2010)

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See detailOperational multipartite entanglement classes for symmetric photonic qubit states
Kiesel, N.; Wieczorek, W.; Krins, Stéphanie ULg et al

Poster (2010)

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See detailL’imagerie Confocale : un Outil Performant pour le Génie des Procédés Biologiques
Louvet, Jean-Noël ULg; Attik, Ghania; Hupont, Sébastien et al

Poster (2010)

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See detailAssessment methodology of the intradermal tuberculosis skin test performed in cattle by field practitioners
Humblet, Marie-France ULg; Walravens, K.; Salandre, O. et al

Poster (2010)

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See detailNormal anatomy and anatomical pitfalls of the distal extremity of the horse with Multi Detector Computed Tomography (MDCT)
Van Thielen, Bert; Murray, Rachel; Van Dooren, JP et al

Poster (2010)

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See detailEffects of methionine sources in interaction with dietary protein content on performance and oxidative status of broiler chickens
Swennen, Q.; Buyse, Johan; Mercier, Yves et al

Poster (2010)

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See detailCA1838, A NANOBODY INHIBITING THE METALLO-β-LACTAMASE VIM-4.
Sohier, Jean ULg; Laurent, Clémentine ULg; Pardon, Els et al

Poster (2010)

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See detailNanobodies as structural probes to investigate the mechanism of fibril formation by the amyloidogenic variants of human lysozyme.
Dumont, Janice ULg; Menzer, Linda ULg; Pardon, Els et al

Poster (2010)

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidose. These ... [more ▼]

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidose. These proteins form extracellular amyloid fibrils that deposit in a wide range of tissues and organs such as liver, spleen and kidneys where they cause damages [1]. It was shown that the D67H and I56T mutations cause a loss in stability and more particularly a loss of global cooperativity of protein [1]. Consequently, under physiologically relevant conditions, these variants can transiently populate a partially unfolded state in which the beta-domain and the C-helix are cooperatively unfolded while the rest of the protein remains native like [1]. The formation of intermolecular interactions between the regions that are unfolded in this intermediate state is likely to be a fundamental trigger of the aggregation process that ultimately leads to the formation and deposition of fibrils in tissues. The binding of three variable domain of camelid antibodies – also named nanobodies - (cAb-HuL 6 [2], cAb-HuL 5 and cAb-HuL 22 [3]) raised against the wild type human lysozyme inhibit in vitro the formation of amyloid fibrils by the lysozyme variants. These three nanobodies bind on different regions of lysozyme and act as Amyloid fibrils inhibitor through different mechanisms. On one hand, cAb-HuL 6 and cAb-HuL 22 stabilize the native state of the lysozyme variants thus restoring the global cooperativity characteristic of the wild-type protein. On the other, cAb-HuL 5 probably acts by binding soluble prefibrillar aggregates. In the present work, sixteen other nanobodies specific of human lysozyme have been generated. Competition experiments have shown that they bind to five non overlapping epitopes. The effects of the binding of these nanobodies on the stability of the D67H variant of human lysozyme and on its aggregation into amyloid fibrils will be discussed. [less ▲]

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See detailPhysiological indicators of dehydration in broiler chickens
Vanderhasselt, R.; Sprenger, M.; Everaert, Nadia ULg et al

Poster (2010)

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See detailVapor phase doping for ultra shallow junction formation in advanced Si CMOS devices
Shimizu, Yasuo; Nguyen, Ngoc Duy ULg; Jiang, Sijia et al

Poster (2010)

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See detailA structural study of natural olivine-type phosphates
Hatert, Frédéric ULg

Poster (2010)

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See detailCamelid single-domain antibody fragments as structural probes to study the mechanism of human lysozyme fibrils formation
Dumont, Janice ULg; Pardon, Els; Menzer, Linda ULg et al

Poster (2010)

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidosis. These ... [more ▼]

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidosis. These proteins form extracellular amyloid fibrils that deposit in a wide range of tissues and organs such as liver, spleen and kidneys where they cause damages [1]. It was shown that the D67H and I56T mutations cause a loss in stability and more particularly a loss of global cooperativity of protein [1]. Consequently, under physiologically relevant conditions, these variants can transiently populate a partially unfolded state in which the beta-domain and the C-helix are cooperatively unfolded while the rest of the protein remains native like [1]. The formation of intermolecular interactions between the regions that are unfolded in this intermediate state is likely to be a fundamental trigger of the aggregation process that ultimately leads to the formation and deposition of fibrils in tissues. The binding of three variable domain of camelid antibodies – also named nanobodies - (cAb-HuL 6 [2], cAb-HuL 5 and cAb-HuL 22 [3]) raised against the wild type human lysozyme inhibit in vitro the formation of amyloid fibrils by the lysozyme variants. These three nanobodies bind on different regions of lysozyme and act as amyloid fibrils inhibitor through different mechanisms. On one hand, cAb-HuL 6 and cAb-HuL 22 stabilize the native state of the lysozyme variants thus restoring the global cooperativity characteristic of the wild-type protein. On the other, cAb-HuL 5 probably acts by binding soluble prefibrillar aggregates. In the present work, sixteen other nanobodies specific of human lysozyme have been generated. Competition experiments have shown that they bind to five non overlapping epitopes. The effects of the binding of these nanobodies on the stability of the D67H variant of human lysozyme and on its aggregation into amyloid fibrils will be discussed. References [1] Dumoulin et al, (2006) Acc. Chem. Res, 39, 603-610. [2] Dumoulin et al, (2003) Nature, 424, 783-788. [3] Chan et al. (2008) Biochemistry, 47,11041-11054. [less ▲]

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See detailEffet de la puissance micro-ondes sur la composition chimique de l'huile essentielle de l'Eucalyptus globulus
Ramdani, M.; Fadel, O.; Mouni, L. et al

Poster (2010)

Dans le but de mettre au point les conditions optimales d’extraction des huiles essentielles sous micro-ondes, nous avons effectué une série de travaux en contrôlant l’effet de plusieurs facteurs sur la ... [more ▼]

Dans le but de mettre au point les conditions optimales d’extraction des huiles essentielles sous micro-ondes, nous avons effectué une série de travaux en contrôlant l’effet de plusieurs facteurs sur la composition chimique des extraits : présence du solvant, quantité de solvant et puissance des micro-ondes. Dans le cas de l’Eucalyptus globulus récolté à Oujda, nous avons comparé la composition chimique de l’huile essentielle extraite par micro-ondes sous deux puissances différentes : 280W et 700W. 38 composés volatils ont été identifiés par GC/MS et GC/FID. La comparaison de la composition chimique, dans les deux cas de puissance d’extraction (280W et 700W), nous a permis de noter respectivement les résultats suivants : a-pinène (2,41%, 4,06%) ; transpinocarveol (2,94%, 3,28%) ; 1,8-cinéole (0,91%, 1,84%) ; (+)-aromadendrene (2,22%, 2,82) ; globulol (4,63%, 4%) ; g-eudesmol (4,66%, 4,2%) ; b-eudesmol (17,02%, 14,5%) ; 1,2,3,4-tetrahydro-2,3-methano-2,8-dimethoxynaphtalene (4,27%, 4,28%) ; anisole, p(m-chlorophenyl) (0 %, 0,24%). La puissance micro-ondes choisie a un effet direct sur la variation de la composition chimique de l’huile essentielle extraite par micro-ondes de point de vue quantitatif et qualitatif. [less ▲]

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Peer Reviewed
See detailCellular engineering of Bacillus subtilis to enhance the biosynthesis of highly active anti fungal lipopeptides
Guy, J.; Guez, Jean-Sébastien; Coucheny, F. et al

Poster (2010)

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See detailShort-term effects of an organochloride pesticide (endosulfan) on amphibian tadpoles
D'Hooghe, Bastien; Brasseur, Catherine ULg; Kestemont, Patrick et al

Poster (2010)

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See detailMorphology of the suspensory ligament (interosseous muscle III) of the horse
Shikh Al Sook, Mohamad Khir ULg; Espinosa, Jennifer; Piret, Joëlle ULg et al

Poster (2010)

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL ... [more ▼]

Introduction: The injuries of the suspensory ligament (SL) are important causes of lameness and financial losses in the equine industry. Ultrasound examination permitted to visualize some parts of the SL. The significance of “abnormal” findings is however not sufficiently known. Until now, few studies described the relationship between the ultrasonographic appearance and the exact morphology in histological sections. The aim of this study is to develop good techniques for cutting and staining the SL and to improve knowledge about the normal morphology of the SL. Methods: In this study, the SL of eight <sound> horses were collected. The body of the SL was divided in 3 thirds and sampling was realised within each third and between the thirds. The samples were embedded in paraffin or in Tissue-Tek for cryosections. The sections were stained with hematoxylin/eosin or Masson's trichrome. For 3 SL, ultrasounds were performed before sampling. The digital tip was maintained in physiological position owing to a press. Results: Most of the paraffin sections were shredded because of the hardness of the tissue. Cryosection revealed a better preservation of tissues. Only some freezing artifacts (holes) appeared on a few sections. Muscles fibers surrounded by adipose tissue containing blood vessels were present mainly in the proximal and medium third of the SL whereas they were not found in the distal third. The remaining structure look like a tendon and was composed of collagen fibers, stained in green with the Masson's trichrome coloration. Conclusions: This study permitted to develop cutting and staining techniques for the SL and helped to map the adipose, muscular and tendinous parts within the SL. It lays down the bases of subsequent studies that will concern ultrasonographically examined digital tips of sound and pathological horses of different breeds and ages. [less ▲]

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See detailHeat treatment of pigmented materials from es-Skhul (ca. 100 000 B.P., Israël)
Salomon, Hélène ULg; Vignaud, Colette; Coquinot, Yvan et al

Poster (2010)

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See detailDesign, implementation, and tests of the architecture of the radiocommunication system of the OUFTI-1 CubeSat
Marchal, Nicolas; Broun, Valéry; Denis, Amandine ULg et al

Poster (2010)

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See detailHyperfine structure splitting of molecular-iodine transitions near 716 nm
Huet, Nicolas ULg; Krins, Stéphanie ULg; Dubé, P. et al

Poster (2010)

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See detailExperience feed back committee and evaluation of weaknesses and critical
DELGAUDINE, Marie ULg; LENAERTS, Eric ULg; COUCKE, Philippe ULg

Poster (2010)

Radiotherapy is a powerful continuously evolving effective treatment tool. Our aim is to offer the best treatments and assure security for patients and personnel. A proactive quality approach copied from ... [more ▼]

Radiotherapy is a powerful continuously evolving effective treatment tool. Our aim is to offer the best treatments and assure security for patients and personnel. A proactive quality approach copied from the one implemented in the air transport industry has been established in our department. An Experience Feed Back Committee (EFBC) has been set up to identify, record and analyze systematically all reported precursor events. Our final objective is to test and strengthen the security of the organization and the quality of care for patients. [less ▲]

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See detailA close look at the RGS spectra of the O4Ief star Zeta Pup
Rauw, Grégor ULg; Flores, A.; Nazé, Yaël ULg et al

Poster (2010)

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See detailL'exraction par micro-ondes: un choix de qualité
Ramdani, M.; Ghazi, Z.; Fadel, O. et al

Poster (2010)

Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par ... [more ▼]

Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par chauffage classique (extraction par entraînement à la vapeur d’un solvant) et, - L’hydro-distillation activée par micro-ondes. Les MO permettent un gain de temps considérable. Par exemple, dans le cas du lavandula dentata : on note une cinétique 3 fois plus rapide, de plus le rendement est nettement supérieur (1% contre 0,6% par chauffage classique). Dans le cas de la plante fraiche, l’extraction est faite en absence de solvant sous MO. Ceci présente un grand intérêt sur le plan économique et environnemental. De plus, la qualité de l’huile extraite présente généralement une meilleure pureté. L’extraction par MO constitue une technique en accord avec la chimie verte. Grâce à ce procédé, on pourra alors réduire d'environ 60 % les besoins en énergie, ce qui diminuera d'autant les émissions de gaz à effet de serre. [less ▲]

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See detailThe Nociception Coma Scale to assess nociception in disorders of consciousness
Chatelle, Camille ULg; Schnakers, Caroline; VANHAUDENHUYSE, Audrey ULg et al

Poster (2010)

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See detailA 500-yr record of Northern Patagonian environmental changes: Lago Plomo and Lago Bertrand
Fagel, Nathalie ULg; Brix, J.; El Ouahabi, M. et al

Poster (2010)

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See detailSelf-Awareness Therapy (SAT) for Schizophrenic Patients
Boulanger, Marie ULg; Nachtergael, Hilde; Pauly, Marc et al

Poster (2010)

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See detailRestauration des campos rupestres, une physionomie du biome Cerrado menacé par le changement d’usage des terres
Le Stradic, Soizig ULg; Buisson, Elise; Fernandes, Geraldo Wilson

Poster (2010)

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See detailDesign, implementation, and test of a digitally-controlled electrical power supply for the OUFTI-1 nanosatellite
Kolodziej, Marie; Ledent, Philippe; Thirion, Pierre et al

Poster (2010)

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See detailEpidemiologic study of pestivirus infection in both wild and domestic ruminants A survey in the Ubaye Valley (Alpine mountains, France)
Martin, Claire; Duquesne, Véronique; Adam, Gilbert et al

Poster (2010)

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See detailSpatial variability of the Siphonophora along the radial Calvi-Nice (Corsican side)
Collignon, Amandine ULg; Hecq, Jean-Henri ULg; Goffart, Anne ULg

Poster (2010)

The spatial distribution of the Siphonophorae has been examined, in Occidental Mediterranean, along the radial Calvi-Nice, from Calvi up to 30 miles offshore in September 2009. The variation of the ... [more ▼]

The spatial distribution of the Siphonophorae has been examined, in Occidental Mediterranean, along the radial Calvi-Nice, from Calvi up to 30 miles offshore in September 2009. The variation of the abundance of the plankton and of the Siphonophorae was put in relation with the hydrological structures of the Liguro-Provençal front. This structure separates the coastal waters of Atlantic origin from the offshore central Mediterranean waters of deep origin. Globally, the larger abundances of Siphonophorae were present in inshore waters between the front and the coast. Both nectophores (asexual stage) and gonophores and bracts (reproductive stage) of Lensia subtilis occured only in the inshore waters. The nectophores of Chelophyes appendiculata occured at all sampling stations with no significative variation. However, the abundance of the bracts and the gonophores increases when we get closer to the coast. This allows us to hypothesize that the maximum of reproduction is located in waters close to the coast. On the other hand, the larvae abundance of calycophoran siphonophores, in the plankton samples, increase from the coastal waters to the offshore waters with a maximum of abundance at the station located at 25 miles offshore. That contradiction could be explained by plankton net avoidance of small larvae, by current transport or by variable hatching success at the various stations. The larvae of physonect siphonophores are more often found offshore. [less ▲]

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See detailMassive stars, a lifetime of influence
Nazé, Yaël ULg

Poster (2010)

Detailed reference viewed: 6 (4 ULg)