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See detailCamelid single-domain antibody fragments as structural probes to study the mechanism of human lysozyme fibrils formation
Dumont, Janice ULg; Pardon, Els; Menzer, Linda ULg et al

Poster (2010)

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidosis. These ... [more ▼]

Six variants of human lysozyme (single-point mutations I56T, F57I, W64R, D67H and double mutations F57I/T70N, W112R/T70N) are associated with a hereditary non-neuropathic systemic amyloidosis. These proteins form extracellular amyloid fibrils that deposit in a wide range of tissues and organs such as liver, spleen and kidneys where they cause damages [1]. It was shown that the D67H and I56T mutations cause a loss in stability and more particularly a loss of global cooperativity of protein [1]. Consequently, under physiologically relevant conditions, these variants can transiently populate a partially unfolded state in which the beta-domain and the C-helix are cooperatively unfolded while the rest of the protein remains native like [1]. The formation of intermolecular interactions between the regions that are unfolded in this intermediate state is likely to be a fundamental trigger of the aggregation process that ultimately leads to the formation and deposition of fibrils in tissues. The binding of three variable domain of camelid antibodies – also named nanobodies - (cAb-HuL 6 [2], cAb-HuL 5 and cAb-HuL 22 [3]) raised against the wild type human lysozyme inhibit in vitro the formation of amyloid fibrils by the lysozyme variants. These three nanobodies bind on different regions of lysozyme and act as amyloid fibrils inhibitor through different mechanisms. On one hand, cAb-HuL 6 and cAb-HuL 22 stabilize the native state of the lysozyme variants thus restoring the global cooperativity characteristic of the wild-type protein. On the other, cAb-HuL 5 probably acts by binding soluble prefibrillar aggregates. In the present work, sixteen other nanobodies specific of human lysozyme have been generated. Competition experiments have shown that they bind to five non overlapping epitopes. The effects of the binding of these nanobodies on the stability of the D67H variant of human lysozyme and on its aggregation into amyloid fibrils will be discussed. References [1] Dumoulin et al, (2006) Acc. Chem. Res, 39, 603-610. [2] Dumoulin et al, (2003) Nature, 424, 783-788. [3] Chan et al. (2008) Biochemistry, 47,11041-11054. [less ▲]

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See detailMacaronesia: a source of hidden genetic diversity for post-glacial recolonization of western Europe in the leafy liverwort Radula lindenbergiana.
Laenen, Benjamin ULg; Desamore, Aurélie ULg; Devos, Nicolas et al

Poster (2010)

Aim Bryophytes exhibit the lowest rates of endemism among biota in Macaronesia and differ in diversity patterns from angiosperms by the widespread occurrence of endemics within and among archipelagos. In ... [more ▼]

Aim Bryophytes exhibit the lowest rates of endemism among biota in Macaronesia and differ in diversity patterns from angiosperms by the widespread occurrence of endemics within and among archipelagos. In this study, we test the hypothesis that high dispersal ability erodes phylogeographic signal and hampers the chances of diversification in bryophytes using the leafy liverwort Radula lindenbergiana as a model. Location Macaronesia, Europe, South Africa Methods 84 samples were collected across the species distribution range and sequenced at four cpDNA loci (atpB-rbcL, trnG, trnL, and rps4). Phylogenetic reconstructions and Bayesian ancestral area reconstructions were used in combination with population genetic statistics (H, Nst, Fst) to describe the pattern of present genetic diversity in R. lindenbergiana and infer its biogeographic history. Results The two regions with the highest haplotypic diversity are Madeira and the Canary Islands. Ancestral area reconstructions suggest that Macaronesia was colonized at least twice independently and that the haplotypes currently found in Western Europe share a Macaronesian common ancestor. Whilst analysis of molecular variance and Nst statistics indicate that present-day patterns of genetic variation have a globally significant biogeographic component, Fst values among Macaronesian archipelagos, North Africa, and the Iberian Peninsula, were not significant. Main conclusions The apparent lack of speciation amongst Macaronesian bryophytes hides actual patterns of diversification at the molecular level. The occurrence of Canarian endemic haplotypes across several islands, along with the non-significant Fst and Nst among islands, North Africa and the Iberian Peninsula, suggest intense dispersal. The occurrence of endemic haplotypes suggests, however, that dispersal does not completely prevent diversification. The high diversity found among Macaronesian haplotypes, together with the Macaronesian origin of all the haplotypes found in Western Europe, suggests that Macaronesian archipelagos could have served as a refugium during the Quaternary glaciations and as a source for re-colonization of Europe. [less ▲]

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See detailPrimary versus secondary failure following varicella vaccination: implications for interval between two doses-Literature review
Bonanni, Paolo; Gershon, Anne; Gershon, Michael et al

Poster (2010)

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See detailActivité antibactérienne et Technique d'extraction
Ramdani, M.; Benchat, N.; Fadel, O. et al

Poster (2010)

L’évaluation de l’activité antibactérienne fait l’objet de nombreuses études dans le domaine des plantes aromatiques et médicinales. Les résultats obtenus correspondent généralement à une technique ... [more ▼]

L’évaluation de l’activité antibactérienne fait l’objet de nombreuses études dans le domaine des plantes aromatiques et médicinales. Les résultats obtenus correspondent généralement à une technique d’extraction employée. Notre travail a pour objectif d’étudier l’effet de la technique d’extraction sur l’activité antibactérienne des huiles essentielles extraites à partir de certaines plantes aromatiques et médicinales. Nous avons préparé deux échantillons d’huiles essentielles par hydro-distillation conventionnelle et par hydro-distillation assistée par micro-ondes. Une série d’expériences a été effectuée sur un groupe de bactéries : E. coli, Staphylococcus, Klépsilla, Protéus, Bacillus. Les résultats obtenus ont montré que l’activité antibactérienne est nettement supérieur dans le cas du chauffage classique pour E. coli et Bacillus. Ceci montre que la composition chimique est différente pour les deux échantillons d’huiles essentielles. Les travaux effectués confirment que l’activité antibactérienne dépend directement de la technique d’extraction utilisée. [less ▲]

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See detailHeralded entanglement of arbitrary degree in remote atoms by detection of emitted photons
Schilling, U.; Thiel, C.; Solano, E. et al

Poster (2010)

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See detailShort-term effects of an organochloride pesticide (endosulfan) on amphibian tadpoles
D'Hooghe, Bastien; Brasseur, Catherine ULg; Kestemont, Patrick et al

Poster (2010)

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See detailWater dynamics in the soil-plant continuum: which features regulate the uptake?
Lobet, Guillaume ULg; Javaux, M.; Pagès, L. et al

Poster (2010)

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See detailEffet de la puissance micro-ondes sur la composition chimique de l'huile essentielle de l'Eucalyptus globulus
Ramdani, M.; Fadel, O.; Mouni, L. et al

Poster (2010)

Dans le but de mettre au point les conditions optimales d’extraction des huiles essentielles sous micro-ondes, nous avons effectué une série de travaux en contrôlant l’effet de plusieurs facteurs sur la ... [more ▼]

Dans le but de mettre au point les conditions optimales d’extraction des huiles essentielles sous micro-ondes, nous avons effectué une série de travaux en contrôlant l’effet de plusieurs facteurs sur la composition chimique des extraits : présence du solvant, quantité de solvant et puissance des micro-ondes. Dans le cas de l’Eucalyptus globulus récolté à Oujda, nous avons comparé la composition chimique de l’huile essentielle extraite par micro-ondes sous deux puissances différentes : 280W et 700W. 38 composés volatils ont été identifiés par GC/MS et GC/FID. La comparaison de la composition chimique, dans les deux cas de puissance d’extraction (280W et 700W), nous a permis de noter respectivement les résultats suivants : a-pinène (2,41%, 4,06%) ; transpinocarveol (2,94%, 3,28%) ; 1,8-cinéole (0,91%, 1,84%) ; (+)-aromadendrene (2,22%, 2,82) ; globulol (4,63%, 4%) ; g-eudesmol (4,66%, 4,2%) ; b-eudesmol (17,02%, 14,5%) ; 1,2,3,4-tetrahydro-2,3-methano-2,8-dimethoxynaphtalene (4,27%, 4,28%) ; anisole, p(m-chlorophenyl) (0 %, 0,24%). La puissance micro-ondes choisie a un effet direct sur la variation de la composition chimique de l’huile essentielle extraite par micro-ondes de point de vue quantitatif et qualitatif. [less ▲]

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See detailResidual stress measurement in dental prostheses by hole-drilling.
Mainjot, Amélie ULg; Schajer, Gary; Vanheusden, Alain ULg et al

Poster (2010)

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See detailL'exraction par micro-ondes: un choix de qualité
Ramdani, M.; Ghazi, Z.; Fadel, O. et al

Poster (2010)

Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par ... [more ▼]

Le but de ce travail est de comparer l’extraction faite par chauffage classique (CC) avec celle réalisée sous micro-ondes (MO). Deux modes d’extraction ont été réalisés : - L’hydro-distillation par chauffage classique (extraction par entraînement à la vapeur d’un solvant) et, - L’hydro-distillation activée par micro-ondes. Les MO permettent un gain de temps considérable. Par exemple, dans le cas du lavandula dentata : on note une cinétique 3 fois plus rapide, de plus le rendement est nettement supérieur (1% contre 0,6% par chauffage classique). Dans le cas de la plante fraiche, l’extraction est faite en absence de solvant sous MO. Ceci présente un grand intérêt sur le plan économique et environnemental. De plus, la qualité de l’huile extraite présente généralement une meilleure pureté. L’extraction par MO constitue une technique en accord avec la chimie verte. Grâce à ce procédé, on pourra alors réduire d'environ 60 % les besoins en énergie, ce qui diminuera d'autant les émissions de gaz à effet de serre. [less ▲]

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See detailVolumetric Oxygen Transfer Coefficient and Foaming in Inverse Fluidised Bed Recycle reactor for biosurfactants production
Nikov; Fahim, S.; Dimitrov, K. et al

Poster (2010)

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See detailComparison of hydrodynamic parameters obtained by 2D measurements in a flat rectangular column with X-ray radiography and data from literature on 3D columns
Calvo, Sébastien ULg; Beugre, Djomice Antoine ULg; Crine, Michel ULg et al

Poster (2010)

Hydrodynamic tests are performed in a flat rectangular column packed with two sheets of Mellapak 250 Y (Sulzer structured packing). Measurements are realized with a single phase liquid flow as well as ... [more ▼]

Hydrodynamic tests are performed in a flat rectangular column packed with two sheets of Mellapak 250 Y (Sulzer structured packing). Measurements are realized with a single phase liquid flow as well as with a gas-liquid counter-current flow. Hydrodynamic parameters are compared to experimental results obtained in 3D columns found in the literature, as well as to literature model predictions and to values computed using an in-house Lattice Boltzmann CFD simulation code. [less ▲]

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See detailA close look at the RGS spectra of the O4Ief star Zeta Pup
Rauw, Grégor ULg; Flores, A.; Nazé, Yaël ULg et al

Poster (2010)

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See detailTraces metal inputs in the Misten bog (East Belgium): Level of contamination and spatial variability
Allan, Mohammed ULg; Fagel, Nathalie ULg; De Vleeschouwer, François et al

Poster (2010)

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See detailA 500-yr record of Northern Patagonian environmental changes: Lago Plomo and Lago Bertrand
Fagel, Nathalie ULg; Brix, J.; El Ouahabi, M. et al

Poster (2010)

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See detailRestauration des campos rupestres, une physionomie du biome Cerrado menacé par le changement d’usage des terres
Le Stradic, Soizig ULg; Buisson, Elise; Fernandes, Geraldo Wilson

Poster (2010)

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See detailSelf-Awareness Therapy (SAT) for Schizophrenic Patients
Boulanger, Marie ULg; Nachtergael, Hilde; Pauly, Marc et al

Poster (2010)

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See detailExploration of perceptual and motor inhibition in children with traumatic brain injury
Catale, Corinne ULg; Lejeune, Caroline ULg; Meulemans, Thierry ULg

Poster (2010)

Inhibitory control, which is commonly considered as one of the essential components of executive functions (Barkley, 1997; Miyake et al., 2000), is not considered as an unitary construct (for example ... [more ▼]

Inhibitory control, which is commonly considered as one of the essential components of executive functions (Barkley, 1997; Miyake et al., 2000), is not considered as an unitary construct (for example, Friedman & Miyake, 2004; Nassauer & Halperin, 2003). In this study, perceptual and motor inhibition were studied in 12 children who had sustained a moderate to severe traumatic brain injury and in 24 matched control children, with the Conflict Resolution task (Nassauer & Halperin, 2003). The perceptual inhibition task required the children to respond to the direction of an arrow while ignoring the conflicting arrow location. In the motor inhibition task, the subject had to press a key corresponding to the opposite direction of a centrally located arrow. Direct comparisons of inhibition performances between traumatic brain injury children and matched controlled subjects showed a specific and disproportionate impairment for motor inhibition (compared to perceptive inhibition) in the traumatic brain injury children, suggesting that inhibition processes might be differentially impaired after traumatic brain injury in children. Interestingly, impairments in behavioural inhibition in day-to-day activities (as reported by the parents), was found to strongly correlate with both types of inhibition. [less ▲]

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See detailGeneration of all Symmetric and all Total Angular Momentum Eigenstates in Remote Qubits
Maser, A.; Schilling, U.; Bastin, Thierry ULg et al

Poster (2010)

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See detailEpidemiologic study of pestivirus infection in both wild and domestic ruminants A survey in the Ubaye Valley (Alpine mountains, France)
Martin, Claire; Duquesne, Véronique; Adam, Gilbert et al

Poster (2010)

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See detailMutliproxy investigation of climatic and anthropogenic changes in a Baltic bog (N. Poland) during the last millennium
De Vleeschouwer, François; Fagel, Nathalie ULg; Piotrowska, Natalia et al

Poster (2010)

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See detailFirst man in the eastern Finland : an Early Mesolithic inland site with a red-ochre grave in Joensuu Rahakangas
Pesonen, Petro; Hertell, Esa; Nyholm, Mikael et al

Poster (2010)

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See detailApproaches for assessing potential impacts of thyroid hormone disrupting chemicals in wild sea bass (Dicentrarchus labrax)
Schnitzler, Joseph ULg; Klaren, Peter; Thomé, Jean-Pierre ULg et al

Poster (2010)

Organic compounds such as pesticides and polychlorobiphenyls (PCBs) are well described endocrine disrupters; of particular interest are effects on thyroid function. To assess the ability of occurring ... [more ▼]

Organic compounds such as pesticides and polychlorobiphenyls (PCBs) are well described endocrine disrupters; of particular interest are effects on thyroid function. To assess the ability of occurring pollutants in European coastal areas to disrupt the thyroid system in sea bass, a field study in the major European estuaries, namely, the Scheldt, the Seine, the Loire, the Charente and the Garonne has been conducted. Several thyroid endpoints were simultaneously examined. Diameter of follicles and the epithelial cell heights give an indication on the production and secretion activity of the thyroid gland. The activity of enzymes involved in the metabolism (deiodination, glucuronidation and sulfatation) of thyroid hormones was analyzed. Finally thyroxine (T4) and triiodothyronine (T3) concentrations were analyzed in muscle by radioimmunoassay. The combined use of all those endpoints gives us an overview of the complex thyroid hormone system. PCB concentration in wild fish varied from 10 to 100 ng g-1 wet weight (ww) depending on length and location. Each region has its own contamination levels and profiles reflecting the contribution of pollutants from rivers. The contamination levels were as follows: the highest concentrations were measured in individuals collected from the coastal region near the Scheldt > Seine > Loire > Charente and the lowest levels were observed in sea bass from coastal regions near the Garonne. Measurements of metabolic activity revealed an altered hepatic T4 outer ring deiodinase whereas T4 sulfatation and T4 glucuronidation were not affected. Owing to the extensive autoregulatory feedback at both central and peripheral levels, the thyroid hormone concentrations were preserved despite the PCB induced changes in thyroid hormone dynamics. [less ▲]

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See detailMultiproxy, multicore palaeoenvironmental study during the last millennium in the Misten peat bog (Hautes Fagnes, East Belgium).
De Vleeschouwer, François; Fagel, Nathalie ULg; Mauquoy, D. et al

Poster (2010)

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See detailStudy of the surface and membrane properties of a bolaform surfactant issued from alkenyl D-xyloside
Deleu, Magali ULg; Damez, Céline; Gatard, Sylvain et al

Poster (2010)

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See detailRELEVANCE OF ACCELERATION AND GRAVITY POWER PROFILING
Jidovtseff, Boris ULg; Harris, Nigel; Cronin, John et al

Poster (2010)

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See detailDéfinition d’indices successionnels pour la caractérisation de la dynamique post-culturale
Bangirinama, F; Bigendako, M J; Lejoly, J et al

Poster (2010)

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See detailStatistical approach of monomer diversity in nonribosomal peptides
Caboche, S.; Leclere, V.; Pupin, M. et al

Poster (2010)

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See detailDecadal changes of carbon dioxide in the Scheldt estuary
Borges, Alberto ULg; Middelburg, J. J.

Poster (2010)

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See detailL'organométallique au service des extraits naturels
Ramdani, R.; Ghazi, Z.; Fadel, O. et al

Poster (2010)

Le Maroc est considéré parmi les pays méditerranéens les plus riches en ressources naturelles, notamment, les plantes aromatiques et médicinales. La valorisation des extraits naturels passe soit par des ... [more ▼]

Le Maroc est considéré parmi les pays méditerranéens les plus riches en ressources naturelles, notamment, les plantes aromatiques et médicinales. La valorisation des extraits naturels passe soit par des échantillons à l’état brut, soit en les transformant en d’autres structures chimiques nouvelles. [less ▲]

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See detailGene expression of the lipoxygenase pathway in a tomato species tolerant to salt stress
Ghars, Mohamed Ali ULg; Muhovski, Y.; Ghanem, M. et al

Poster (2010)

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See detailhCG: a pregnancy-related hormone stimulating angiogenesis and pericyte recruitment
Berndt, S; Blacher, Silvia ULg; Perrier d’Hauterive, S et al

Poster (2010)

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See detailOut of Africa: north-westwards Pleistocene expansions of the heather Erica arborea
Desamore, Aurélie ULg

Poster (2010)

ntroduction Erica arborea L. is a dominant element of the circum-Mediterranean region. Its broad, disjunct distribution, ranging from Macaronesia to eastern Africa, is consistent with the fragmentation of ... [more ▼]

ntroduction Erica arborea L. is a dominant element of the circum-Mediterranean region. Its broad, disjunct distribution, ranging from Macaronesia to eastern Africa, is consistent with the fragmentation of the evergreen tropical forests that dominated Europe and North Africa in the Tertiary. (fig.1) Aim Is the current disjunct distribution of E. arborea a relict of a once wider distribution or a recent range expansion in response to the establishment of suitable conditions ? [less ▲]

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See detailInfluence of environmental factors on lipopeptide production of a spontaneous mutant strain of Bacillus subtilis ATCC 21332
Fahim, S.; Dimitrov, K.; Gancel, F. et al

Poster (2010)

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See detailTHE NORMAL ANATOMICAL DEVELOPMENT OF THE FOAL FOOT DESCRIBED WITH MULTI DETECTOR COMPUTED TOMOGRAPHY (MDCT)
Van Thielen, B.; Murray, R.; Van Dooren, J. P. et al

Poster (2010)

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See detailAssessment methodology of the intradermal tuberculosis skin test performed in cattle by field practitioners
Humblet, Marie-France ULg; Walravens, K.; Salandre, O. et al

Poster (2010)

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See detailNumerical simulations of the wind of magnetic massive star HD191612
ud-Doula, Asif; Nazé, Yaël ULg

Poster (2010)

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See detailChilling of carcasses from double muscled cattle: time-temperature evolution and predictive modelling of growth of Listeria monocytogenes and Clostridium perfringens
Delhalle, Laurent ULg; Collignon, Bertrand; Dehard, Sandrine ULg et al

Poster (2010)

The time/temperature combination during carcass chilling is of concern in order to avoid bacterial growth. The chilling speed is lower in carcasses with high muscular development such as large cattle from ... [more ▼]

The time/temperature combination during carcass chilling is of concern in order to avoid bacterial growth. The chilling speed is lower in carcasses with high muscular development such as large cattle from the Belgian Blue breed. Three slaughterhouses were selected for temperature and pH measurements during the chilling process at 6 different days on 4 half carcasses in order to obtain representative data from heavy carcasses with high muscular development. Predictive microbiology was used to evaluate the potential growth of Listeria monocytogenes and Clostridium perfringens on the surface and in the depth of the carcasses. The gamma concept was chosen as secondary model taking into account the effect of temperature, pH and water activity on the selected bacteria during the chilling process. The predicted growth potential of Listeria monocytogenes is influenced by the different environmental conditions of the selected slaughterhouses and could reach 1.4 log CFU/cm² after the chilling process. The potential growth of Clostridium perfringens is limited due to unfavourable conditions during the first hours and to low temperature later. It can be concluded that when the initial level of contaminating bacteria is not excessive the speed at which the carcass is currently chilled is sufficient to limit the growth of these two pathogens and to ensure the product quality. [less ▲]

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See detailModeling post-combustion CO2 capture with amine solvents
Léonard, Grégoire ULg; Heyen, Georges ULg

Poster (2010)

In order to avoid the emission of large amounts of greenhouse gas, CO2 capture in fossil fuel power plants and subsequent underground CO2 sequestration is studied. The capture occurs by reactive CO2 ... [more ▼]

In order to avoid the emission of large amounts of greenhouse gas, CO2 capture in fossil fuel power plants and subsequent underground CO2 sequestration is studied. The capture occurs by reactive CO2 absorption into chemical solvent systems at moderate temperature (~50°C) followed by solvent regeneration at higher temperature (~120°C). So far, the most employed solvent for acid gas capture is monoethanolamine (MEA). One main drawback of this technology is the high energy consumption necessary to regenerate the solvent. In the present work, the CO2 capture process with MEA is modeled using the simulation tool Aspen Plus®. The base case process is optimized and some process improvements are studied that imply a significant decrease of the process exergy consumption. [less ▲]

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See detailMonitoring endocrine profiles for prediction of stillbirth in Holstein-Friesian dairy cattle
Szenci, O.; Bajcsy, A. C.; Bryd, E. et al

Poster (2010)

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See detailDiagnostic de l’exploration botanique des Caesalpinioideae et Mimosoideae de l’Afrique centrale
Ndayishimiye, J; Bamba, I; Stoffelen, P et al

Poster (2010)

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See detailAutomatic stimulus-induced medial premotor cortex activation without perception or action
D'Ostilio, Kevin ULg; Garraux, Gaëtan ULg

Poster (2010)

Evidence from functional imaging studies suggests that well-established stimulus-action associations may induce an automatic activation of the motor preparation system even when there is no intention to ... [more ▼]

Evidence from functional imaging studies suggests that well-established stimulus-action associations may induce an automatic activation of the motor preparation system even when there is no intention to make the associated movement (Grezes & Decety, 2002). Here we investigated whether this automatic motor activation can also be elicited by visual stimuli that are not consciously perceived. However, previous subliminal masked experiments used supraliminal response-target in such a way that unconscious mechanisms were inferred from the accumulation of the effect of the subliminal masked stimulus and the motor response. Here, to investigate neural correlates of unconscious process induced by subliminal stimuli, we used event-related BOLD fMRI at 3T to record brain activity in 24 healthy volunteers (mean age: 21 ± 2 years) as they performed a subliminal priming task (see Eimer & Schlaghecken, 1998). In this visuomotor task, participants were asked to make speeded button presses with the left or right hand following double leftward (<< <<) or rightward (>> >>) pointing arrows, which were preceded by a masked subliminal prime of 17 ms (compatible/incompatible arrows or neutral stimulus). Reaction time analysis revealed the classical positive compatibility effect (PCE), mainly shorter reaction times for compatible (mean RT: 369±38 ms) than for incompatible (mean RT: 383± 30 ms) in comparison to neutral trials (mean RT: 375± 38 ms). In a prime identification task, subjects’ performance was at chance level for primes presented for 17 ms, suggesting that the prime was not consciously perceived in the main experiment. Theses behavioral results suggest an automatic and unconscious motor activation induced by the prime. The responded stimuli were randomly intermixed with non-responded stimuli (0 0) with the assumption that the subliminal arrow prime also elicited an automatic motor activation in these passive trials as in responded trials. Imaging analysis of these non-responded trials showed first that activation was mainly restricted to posterior brain areas when using a subliminal stimulus that has not been previously associated with a motor response. Second, when the subliminal stimulus has been strongly associated with a motor response, this activation extended to rostral brain regions classically involved in motor preparation as the SMA, the premotor cortex and the striatum (cluster level p< 0.05 corrected). This finding corroborates the involvement of a fronto-striatal network, especially of the SMA in automatic and unconscious motor activation. [less ▲]

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See detailHD 150136: towards one of the most massive systems?
Mahy, Laurent ULg; Gosset, Eric ULg; Rauw, Grégor ULg et al

Poster (2010)

The improvement of the astronomical instrumentation allowed scientists to develop new analysis techniques to investigate binary and multiple stars. In this context, we present the preliminary results of ... [more ▼]

The improvement of the astronomical instrumentation allowed scientists to develop new analysis techniques to investigate binary and multiple stars. In this context, we present the preliminary results of an intense monitoring devoted to the triple system, HD 150136. Supposed to be the nearest O3 star (1.3 kpc), this object is also composed of two other early O-type stars, making it one of the most massive systems known until now (~ 134Mʘ). This multiple star is a non-thermal radio emitter and presents a colliding wind interaction zone. To determine the physical parameters of this system, we applied a disentangling program to study individually the three components. It allows us to revise their spectral classification and to derive a new orbital solution for the short-period system but also the first one for the entire system. [less ▲]

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See detailA structural study of natural olivine-type phosphates
Hatert, Frédéric ULg

Poster (2010)

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See detailNormal anatomy and anatomical pitfalls of the distal extremity of the horse with Multi Detector Computed Tomography (MDCT)
Van Thielen, Bert; Murray, Rachel; Van Dooren, JP et al

Poster (2010)

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See detailHeat treatment of pigmented materials from es-Skhul (ca. 100 000 B.P., Israël)
Salomon, Hélène ULg; Vignaud, Colette; Coquinot, Yvan et al

Poster (2010)

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See detailCharacterisation of B1 metallo-beta-lactamase inhibition by VHHs
Sohier, Jean ULg; Laurent, Clémentine ULg; Chevigné, Andy et al

Poster (2010)

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See detailA STUDY OF THE CORRELATION BETWEEN MACROTEXTURE AND THE TIRE/PAVEMENT CLOSE PROXIMITY NOISE
Luong, Jeanne ULg; Bueno, M.; Teran, F. et al

Poster (2010)

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See detailWeignhing risk factors associated with bee colony collapse disorder by classification and regression tree analysis
Vanengelsdorp, D.; Speybroeck, N.; Evans, J. et al

Poster (2010)

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See detailSpatial variability of the Siphonophora along the radial Calvi-Nice (Corsican side)
Collignon, Amandine ULg; Hecq, Jean-Henri ULg; Goffart, Anne ULg

Poster (2010)

The spatial distribution of the Siphonophorae has been examined, in Occidental Mediterranean, along the radial Calvi-Nice, from Calvi up to 30 miles offshore in September 2009. The variation of the ... [more ▼]

The spatial distribution of the Siphonophorae has been examined, in Occidental Mediterranean, along the radial Calvi-Nice, from Calvi up to 30 miles offshore in September 2009. The variation of the abundance of the plankton and of the Siphonophorae was put in relation with the hydrological structures of the Liguro-Provençal front. This structure separates the coastal waters of Atlantic origin from the offshore central Mediterranean waters of deep origin. Globally, the larger abundances of Siphonophorae were present in inshore waters between the front and the coast. Both nectophores (asexual stage) and gonophores and bracts (reproductive stage) of Lensia subtilis occured only in the inshore waters. The nectophores of Chelophyes appendiculata occured at all sampling stations with no significative variation. However, the abundance of the bracts and the gonophores increases when we get closer to the coast. This allows us to hypothesize that the maximum of reproduction is located in waters close to the coast. On the other hand, the larvae abundance of calycophoran siphonophores, in the plankton samples, increase from the coastal waters to the offshore waters with a maximum of abundance at the station located at 25 miles offshore. That contradiction could be explained by plankton net avoidance of small larvae, by current transport or by variable hatching success at the various stations. The larvae of physonect siphonophores are more often found offshore. [less ▲]

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See detailThe Venus OH Nightglow Distribution
Soret, Lauriane; Gérard, Jean-Claude ULg; Piccioni, G. et al

Poster (2010)

The study shows that the OH emission is highly variable, ranging from less than 20 kR to about 2 MR. The peak brightness appears to decrease away from the antisolar point even if the variability at a ... [more ▼]

The study shows that the OH emission is highly variable, ranging from less than 20 kR to about 2 MR. The peak brightness appears to decrease away from the antisolar point even if the variability at a given location is very strong. By contrast, although the OH peak altitude also appears to be variable, it shows no dependence with the location on the nightside of Venus. Some correlation between simultaneous observations of the intensity of the OH and the O2(a1Δ) emissions has also been detected, presumably because atomic oxygen is a common precursor to the formation of O2(a1Δ) and O3, whose reaction with H produces excited OH. Anyway, it is important to keep in mind that horizontal transport plays an important role in the redistribution of photochemically produced species such as O, O3 and minor long-lived species and possibly explains some of the variability of the OH emission and its brightness relative to O2(a1 ). [less ▲]

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See detailBacillomycin D and mycosubtilin, antimicrobial lipopeptides from Bacillus subtilis. A comparative analysis of their interfacial properties
Nasir, Mehmet Nail ULg; Besson, Françoise

Poster (2010)

Bacillus subtilis strains produce antimicrobial lipopeptides using non ribosomal peptide synthesis pathway. Among them, those belonging to iturinic family have an important place. This family is ... [more ▼]

Bacillus subtilis strains produce antimicrobial lipopeptides using non ribosomal peptide synthesis pathway. Among them, those belonging to iturinic family have an important place. This family is characterized by a peptide ring with a constant LDDLLDL chiral amino acid sequence cycled by a -fatty acid with a variable acyl chain. The iturinic lipopeptides exhibit their biological activities on the plasma membrane of the microbial cells. It has been demonstrated that they could be active on pathogenic strains which are resistant to currently-used therapeutic agents. Despite their interesting antimicrobial properties, there is limited number of works on their interactions with plasma membranes in order to understand their mode of action. Langmuir monolayers at the air-water interface are a useful tool to analyze the interactions between antimicrobial lipopetides and membranes. We therefore investigated iturinic lipopeptides-membrane interactions using Langmuir monolayers as membrane model. Our work focused on two iturinics, mycosubtilin and bacillomycin D, differing by their peptide ring (Figure 1). In the first step, we investigated their interfacial properties using tensiometry measurements and polarization modulation infrared reflexion absorption spectroscopy (PM-IRRAS). We found that the ability to form an interfacial film for two lipopeptides was different depending on the nature of the subphase. Moreover, their conformations at the air-water interface were determined as turns. In the second step, we investigated the insertion of the mycosubtilin and bacillomycin D in the lipid monolayers by determining their exclusion surface pressures. Our results showed that the insertion depends on the nature of the lipid film hence a membrane-compostion-dependant action of the two antimicrobial compounds could be explained. [less ▲]

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See detailIncreased mitosis in the bleached gastrodermis of the sea anemone A. pallida
Fransolet, David ULg; Roberty, Stéphane ULg; Plumier, Jean-Christophe ULg

Poster (2010)

Today, coral bleaching represents a major concern for marine biologists, especially considering the upsurge of this phenomenon possibly linked to climate change. Bleached corals, deprived of most of their ... [more ▼]

Today, coral bleaching represents a major concern for marine biologists, especially considering the upsurge of this phenomenon possibly linked to climate change. Bleached corals, deprived of most of their energy incomes, may show a partial or total mortality, which ultimately lead to shifts in reef communities. Studies focusing on cellular bleaching mechanisms have shown different ways by which algae may be expelled from gastrodermal host cells. Among those mechanisms, major emphasis has been put on host cell death, most probably due to both apoptosis and necrosis. Recovering gastrodermis is then expected to undergo regeneration process in order to be reinfected by new algae. We describe here this regeneration process in the bleached sea anemone model A. pallida. [less ▲]

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See detailCan Pleistocene refuge theory explain within-species patterns of genetic diversity in African lowland rainforest trees?
Heuertz, Myriam; Savolainen, Vincent; Budde, Katharina et al

Poster (2010)

Pleistocene refuge theory holds that regions which nowadays harbour high numbers of endemic species correspond to forest refuges, where rainforest persisted through periods of adverse climatic conditions ... [more ▼]

Pleistocene refuge theory holds that regions which nowadays harbour high numbers of endemic species correspond to forest refuges, where rainforest persisted through periods of adverse climatic conditions. In order to test this theory, we surveyed geographical patterns of genetic diversity based on chloroplast DNA sequences in 15 rainforest tree species from 12 plant families in Atlantic Equatorial Africa. We found frequent geographic structure in the data sets, but no consistent pattern of genetic structure due to refugia. Species with gravity-dispersed oily seeds display low polymorphism whilst those with divergent lineages or ancient species display high polymorphism. Phylogeographical signals often correspond to taxa with divergent lineages. [less ▲]

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See detailImpact of tree species on the distribution of alkaliextractable Si in a Cambisol
Cornelis, Jean-Thomas ULg; Ranger, Jacques; Delvaux, Bruno

Poster (2010)

Abstract : In terrestrial ecosystems, silicon (Si) uptake by higher plants induces biogenic silica (BSi) deposits in leaves, which contribute to the amorphous silica (ASi) pool in soil through litter-fall ... [more ▼]

Abstract : In terrestrial ecosystems, silicon (Si) uptake by higher plants induces biogenic silica (BSi) deposits in leaves, which contribute to the amorphous silica (ASi) pool in soil through litter-fall. In forests, the ASi pool, including BSi, is ubiquitous and a substantial component of soils, which might influence the Si mass-balance at watershed scale. Here, we examined the distribution of ASi pool, estimated by alkaline dissolution (alkali-extractable Si), in an acid brown soil under three common European tree species in identical soil and climate conditions in order (i) to study how the Si recycling by tree species impacts the ASi pool in soil, and (ii) to identify the different constituents of the alkali-extractable Si pool in soil. We therefore quantified the ASi concentration with alkaline extraction (Na2CO3, 0.1M), the Si adsorbed onto poorly crystalline Fe oxides by oxalate extraction and the “plant-available Si” by CaCl2 extraction. In humus layer, the alkali-extractable Si concentration (mg SiO2 g-1) significantly decreases in the sequence: Douglas fir (14.5±0.65) > European beech (11.8±0.30) > Black pine (5.4±0.31). Below 15 cm soil depth, the alkali-extractable Si concentration is not significantly different between tree species. For each tree species, the alkali-extractable Si concentration in soil decreases from the humus layer to 15 cm depth and then slightly increases from 15 to 75 cm depth. Our data clearly show that tree species can impact the ASi content in topsoil (humus layer - 15 cm) through different Si uptake rates. Indeed, various Si recycling by forest vegetation imply different rates of BSi accumulation in leaves and then, different rates of BSi restitution on topsoil. In mineral layers, pedogenic processes play an important role in the ASi distribution given the alkali-extractable Si pool is mostly influenced by BSi dissolution, stable BSi preservation/translocation and secondarily by Si adsorption onto active amorphous Fe oxide surface. [less ▲]

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See detailMaillage 3D par imagerie RMN d’un grain de maïs et modélisation des transferts de chaleur et de masse durant son séchage
Janas, Sébastien ULg; Boutry, Sébastien; Vander Elst, Luce et al

Poster (2010)

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See detailRedistribution of fibrillarin following treatment of human bladder carcinoma cells with aptatone
Jamison, James; Gilloteaux, Jacques; Perlaky, L et al

Poster (2010)

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See detailForensic science technique applied for calculation of kinship index
Bömcke, Elisabeth ULg; Gengler, Nicolas ULg

Poster (2010)

Implementing conservation strategies needs the knowledge of relationships inside the concerned population. The aim of this study was to find tools to help scientists and breeders to manage endangered ... [more ▼]

Implementing conservation strategies needs the knowledge of relationships inside the concerned population. The aim of this study was to find tools to help scientists and breeders to manage endangered populations or populations with missing pedigree information. The animal genetics literature often seems unaware of relevant developments in human genetics (and conversely). In this study, an approach called Familial Searching was tested. This is used in forensic science, in addition to matching DNA evidence directly to criminal profiles, to search for people (present in a database) who are related to an individual that left DNA evidence at a scene of crime. This method is based on the calculation of likelihood ratios (LR) between genotype of an individual and genotypes of each other individuals of the database. In order to decrease the number of comparisons, the available pedigree information was used as ‘local’ prior information, i.e. relating to specific pairs of individuals. General knowledge about the studied population (e.g., generation interval, sexual maturity) was considered as ‘global’ prior information. Including prior information reduced the number of comparisons from over 50%. Results showed that the parents were always classified into the 4 highest LR. This method simplified parentage verifications, it allowed the detection of 90% of false parentage (LR=0). It also allowed to create new links in the pedigree through detection of unregistered parents. The method was tested on the Skyros pony, an indigenous Greek breed. For this breed, partial pedigree information was available, and 99 individuals were genotyped at 16 microsatellite loci. The method allowed to check about 2500 possible parent-child combinations, three registered parentages were considered as incorrect and one non-recorded parentage was detected. The method will now be tested on other breed and with other markers, e.g. SNPs. [less ▲]

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See detailGlobal balances as a basis for ethical statements
Fayyaz, Sara; Kopriwa, Nicole; Hillerbrand, Rafaela et al

Poster (2010)

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