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See detailL’Inventaire Systémique de Qualité de Vie pour Enfants (ISQV-E©) : Etude de validation
Missotten, Pierre ULg; Lemétayer, Fabienne; Spitz, Elisabeth et al

Poster (2008, May)

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See detailTime Reversal of Atomic Matter Waves
Martin, John ULg; Georgeot, Bertrand; Shepelyansky

Poster (2008, May)

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See detailNew results on the UV Io footprint morphology and brightness
Bonfond, Bertrand ULg; Grodent, Denis ULg; Gérard, Jean-Claude ULg et al

Poster (2008, April 18)

The Io UV footprint is an auroral feature on Jupiter caused by the electromagnetic interaction between the satellite Io and the Jovian magnetosphere. The footprint morphology and the spots multiplicity ... [more ▼]

The Io UV footprint is an auroral feature on Jupiter caused by the electromagnetic interaction between the satellite Io and the Jovian magnetosphere. The footprint morphology and the spots multiplicity have been found to vary with the location of Io in the plasma torus. We show recent Hubble Space Telescope (HST) images that reveal a new feature in the footprint: a faint leading spot that appears upstream of the main spot in one hemisphere when Io is close to the opposite border of the torus. A possible interpretation relates the leading spots and one downward secondary spot to electron beams generated by downstream currents in the opposite hemisphere. We also present a 3D model of the Io footprint emissions in the 100 to 170 nm wavelength range. Comparisons between this model and the HST images enable us to study the actual size and shape of the different Io footprint features. It also allows to measure the footprint brightness on the new images with a better estimation of the geometric effects (e.g. limb brightening). The observations presented here provide critical constraints to the Io-plasma torus interaction modeling. [less ▲]

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See detailFunctional Development of Advanced Driver Assistance Systems by means of Driving Simulators
Christen, Fréderic ULg; Benmimoun, A.; Deutschle, S.

Poster (2008, April 16)

Der Beitrag beschreibt die Funktionsentwicklung von Fahrerassistenzsystemen an der Forschungsgesellschaft Kraftfahrwesen mbH Aachen (fka) und am Institut für Kraftfahrwesen (ika) der RWTH Aachen mittels ... [more ▼]

Der Beitrag beschreibt die Funktionsentwicklung von Fahrerassistenzsystemen an der Forschungsgesellschaft Kraftfahrwesen mbH Aachen (fka) und am Institut für Kraftfahrwesen (ika) der RWTH Aachen mittels Fahrsimulatoren. Dabei wird konkret auf die Entwicklung eines Kreuzungsassistenten sowie eines sogenannten KONVOI-Systems eingegangen. Beide Systeme wurden u.a. unter Verwendung des statischen Fahrsimulators InDriveS entwickelt. Der in diesem Beitrag vorgestellte Ansatz eines Kreuzungsassistenten basiert auf Kommunikation: Fahrzeug-Fahrzeug-Kommunikation (C2C) und Infrastruktur-Fahrzeug- Kommunikation (I2C). Hierfür wurden in der Verkehrsfluss- und Fahrsimulation verschiedene Systemvarianten betrachtet, um unterschiedliche Stufen der Systemkomplexität und unter- schiedliche Zeitrahmen für die Realisierung eines solchen Assistenten zu berücksichtigen. Jede dieser Systemvarianten wurde hinsichtlich deren Wirkung auf die Verkehrssicherheit bewertet. Daneben wurde auch die Benutzerakzeptanz unter Berücksichtigung verschiedener Mensch-Maschine-Schnittstellen betrachtet. Die Ergebnisse zeigen, dass die Kommunikationsreichweite der wichtigste Parameter für die Systemauslegung und -spezifikation darstellt. Für die Wirkung des Kreuzungsassistenten auf die Verkehrssicherheit ist in erster Linie der Ausrüstungsgrad entscheidend. Für die Benutzerakzeptanz ist die Detektionsrate von möglichen Konfliktsituationen und die Vermeidung von kritischen Situationen entscheidend. Das dargestellte KONVOI-System ermöglicht die Automatisierung von Nutzfahrzeugkolonnen auf Autobahnen. Neben der Funktionsentwicklung zur automatischen Abstandsregelung und Querführung werden in dem Projekt die Auswirkungen von KONVOIs auf den übrigen Verkehr analysiert und die bei den Fahrern auftretenden Belastungen und die Akzeptanz des Systems untersucht. Begleitend werden rechtliche Aspekte der kommerziellen Nutzung von Lkw-KONVOIs in Deutschland weiterentwickelt. Um die Komplexität der zu entwickelnden Lösungen zur Funktionserweiterung der Fahrzeuge zu bewältigen und eine hohe Zuverlässigkeit der Systeme zu gewährleisten, erfolgt die Systementwicklung mit Hilfe von Simulationswerkzeugen (MATLAB/Simulink, Stateflow, Verkehrsflusssimulation PELOPS und Lkw- Fahrsimulator InDriveS). Abschließend geht der Beitrag auf den neuen dynamischen Fahrsimulator der RWTH Aachen ein. [less ▲]

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See detailNew pH-sensitive flower micelles for potential tumour targeting
Cajot, Sébastien ULg; Van Butsele, Kathy; Jérôme, Christine ULg

Poster (2008, April 16)

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See detailA record negative Greenland ice sheet surface mass balance rate in 2007
Fettweis, Xavier ULg

Poster (2008, April 15)

Results made with the regional climate model MAR show a record surface melt (592 km³/yr = a global sea level rise of 1.6 mm/yr) of the Greenland ice sheet (GrIS) during summer 2007 compared with 1970-2006 ... [more ▼]

Results made with the regional climate model MAR show a record surface melt (592 km³/yr = a global sea level rise of 1.6 mm/yr) of the Greenland ice sheet (GrIS) during summer 2007 compared with 1970-2006. This record melt, detected also in the microwave satellite data, is associated with very low snowfall (508 km³/yr) inducing a negative Surface Mass Balance (SMB) rate of -65 km³/yr. Such a negative simulated SMB rate is unprecedented in the recent Greenland history. The summer 2007 is associated with a positive SST anomaly, a negative 2006-2007 GrIS winter accumulation and anomalous advection of warm air masses over the GrIS. Sensitivity experiments carried out by the MAR model evaluate the impacts of these anomalies on the Greenland climate and SMB. The main impacts of a warmer SST anomaly in the MAR model are more precipitation over Greenland due to an enhanced evaporation above the ocean and, an increase of surface melt induced by the advection of warmer oceanic air (>0°C) into the continent by the atmospheric part of MAR. A negative winter accumulation anomaly exposes ice and old snow (with a lower albedo) earlier than previous years in the ablation zone which significantly increases the melting given the albedo feedback. Finally, changes in the boundaries forcing of the MAR model test the consequence of the anomalous persistent southerly airflow during June and July. [less ▲]

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See detailImpacts of ice sheet mask and resolution on estimating the surface mass balance of the Greenland ice sheet
Fettweis, Xavier ULg

Poster (2008, April 15)

The impacts of the spatial resolution and a Greenland ice sheet (GrIS) mask on modelling the Surface Mass Balance (SMB) are studied with the regional climate model MAR coupled with a complex energy ... [more ▼]

The impacts of the spatial resolution and a Greenland ice sheet (GrIS) mask on modelling the Surface Mass Balance (SMB) are studied with the regional climate model MAR coupled with a complex energy balance/snowpack model. On the one hand, too coarse resolution prevents the model from resolving adequately the steep ice sheet margin and the ablation zone, not wider than 100 km in Greenland, where substantial seasonal melting occurs. The resolution affects also the precipitation modelling. On the other hand, a too large ice sheet mask (i.e. with low-altitude ice pixels in the model, where there is no ice in reality) leads to an overestimation of the run-off. In addition, due to the albedo feedback, biases in the ice sheet mask have also consequences on the surface energy balance. [less ▲]

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See detailThree-dimensional analysis of oceanographic data with the software DIVA
Troupin, Charles ULg; Ouberdous, Mohamed ULg; Machín, Francisco et al

Poster (2008, April 13)

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See detailScreening for chronic kidney disease and other associated risk factors in rural congolese oil company in the Democratic Republic of Congo (DRC).
Sumaili, Ernest; Zinga, Chantal; Nseka, Nazaire et al

Poster (2008, April 10)

This study indicates that CKD and traditional risk factors for CKD are very prevalent in this rural Oil Company. Screenings for these conditions are thus imperative to put high on the health agenda ... [more ▼]

This study indicates that CKD and traditional risk factors for CKD are very prevalent in this rural Oil Company. Screenings for these conditions are thus imperative to put high on the health agenda. Additional studies are needed of the causes of CKD in this company and probably in other areas of the DRC. [less ▲]

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See detailDIVA-4.2.1: presentation of the new features
Troupin, Charles ULg; Machín, Francisco; Ouberdous, Mohamed ULg et al

Poster (2008, April 03)

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See detailDiversité moléculaire des cyanobactéries planctonniques dans les eaux de surface belges
Lara, Yannick ULg; Lambion, Alexandre ULg; Simon, Patricia ULg et al

Poster (2008, April 02)

Les développements massifs de cyanobactéries ou ‘blooms’ sont devenus un phénomène récurrent et de plus en plus important dans les eaux douces du monde entier durant la dernière décennie. Ces ... [more ▼]

Les développements massifs de cyanobactéries ou ‘blooms’ sont devenus un phénomène récurrent et de plus en plus important dans les eaux douces du monde entier durant la dernière décennie. Ces efflorescences présentent des risques potentiels majeurs pour la santé humaine et animale et interfèrent négativement avec l'utilisation des eaux de surface par exemple, pour le captage d'eau potable, les loisirs nautiques, l'irrigation, les exploitations piscicoles. Entre 25 et 70% des blooms sont toxiques. Comme beaucoup de pays la Belgique n'a pas échappé au problème des efflorescences de cyanobactéries toxiques, mais il y a encore relativement peu de données. Durant la dernière décennie, trois projets européens et nationaux (MIDICHIP 1999-2003, B-BLOOMS 2003-2005, B-BLOOMS 2 2007-2011) se sont intéressés à la diversité des cyanobactéries dans les eaux de surfaces belges. Nous présentons ici un arbre phylogénétique élaboré à partir d’ un pool de 249 séquences partielles du gène codant pour l’ARNr 16S obtenu à partir de 31 échantillons d’eaux belges issus de ces projets. Cet arbre représente la mise à jour d’une base de données qui constitue l’inventaire des cyanobactéries d’eaux douces belges. Cette base de données permet le suivi de l’évolution de la diversité observable de ces organismes en Belgique et la surveillance de l’apparition d’espèces tropicales comme conséquence aux changements climatiques globaux. [less ▲]

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See detailCarbon dioxide dynamics in lake Kivu during the dry and wet seasons
Borges, Alberto ULg; Delille, Bruno ULg; Descy, J.-P. et al

Poster (2008, April)

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See detailStudy of the acid hydrolysis of pectin extracted from sugar beet pulp
Valepyn, Emmanuel ULg; Robert, Christelle; Paquot, Michel ULg

Poster (2008, April)

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See detailAutomatic 1958-2007 daily weather pattern classification applied to an analysis of climatic conditions of wildfires in eastern Belgium
Erpicum, Michel ULg; Fettweis, Xavier ULg; Mabille, Georges ULg et al

Poster (2008, April)

The daily atmospheric circulation patterns classification is founded on a 100 km regular grid centred on Belgium. The geopotential heights of 500, 850 and 1000 hPa levels were extracted <br />from the ERA ... [more ▼]

The daily atmospheric circulation patterns classification is founded on a 100 km regular grid centred on Belgium. The geopotential heights of 500, 850 and 1000 hPa levels were extracted <br />from the ERA-40 database on the period 1958-2002 and from ECMWF operational analysis until the end of year 2007. The classification was based on a similarity index calculated on the orientation of exaggerated slopes of different daily geopotential fields. Wildfire occurrences were analyzed in April and September (which are the two months with the most frequent wildfire-days in Belgium) together with monthly frequencies and persistences of daily atmospheric circulation pattern classes as well as with yearly variability of weather climate conditions. [less ▲]

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See detailImpact of cristallinity index on the stability and physical properties of inulin during moisture uptake.
Ronkart, Sébastien; Paquot, Michel ULg; Fougnies, C. et al

Poster (2008, April)

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See detailLe réseau "Mieux manger à l'école" de la Fondation Roi Baudouin : Quelles synergies à l'échelle locale et régionale ?
Van Hoye, Aurélie ULg; Taminiaux, Pascale; Cerisier, Bettina

Poster (2008, April)

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See detailLast century Greenland ice sheet surface mass balance projections from IPCC AR4 global models
Franco, Bruno ULg; Fettweis, Xavier ULg; Erpicum, Michel ULg

Poster (2008, April)

Results from atmosphere-ocean general circulation models (AOGCM's) for the IPCC 4th Assessment Report are used to investigate surface mass balance (SMB) future projections of the Greenland ice sheet (GrIS ... [more ▼]

Results from atmosphere-ocean general circulation models (AOGCM's) for the IPCC 4th Assessment Report are used to investigate surface mass balance (SMB) future projections of the Greenland ice sheet (GrIS). The most efficient models for the GrIS climate modeling are chosen by comparison between the 1970-1999 outputs (averages and trends) from the 20C3M Experiment outputs, and the reanalyses (ECMWF, NCEP) as well as climatologies. The SMB is estimated from the summer temperature (from which is deduced the run-off) and annual snowfall from the well-adapted AOGCM's. It is validated with 1970-1999 results from the regional climate model MAR by interpolating the AOGCM's outputs on the MAR grid. However, large uncertainties remain in these SMB projections due to the simplified physic and coarse AOGCM's resolution. High resolution simulations made with the MAR model (which simulates explicitly the SMB by taking into account the surface feedbacks) forced at its boundaries by a GrIS well-adapted AOGCM could bring more precise brief replies. [less ▲]

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See detailAnalyse bioinformatique de différents marqueurs génétiques pour la caractérisation taxonomique et génotypique des algues bleues.
Lara, Yannick ULg; Wilmotte, Annick ULg

Poster (2008, April)

Les cyanobactéries (ou algues bleues) sont des organismes procaryotes photosynthétiques considérés comme les ancêtres des plastes des végétaux eucaryotes. Présentes dans différents types d’habitats ... [more ▼]

Les cyanobactéries (ou algues bleues) sont des organismes procaryotes photosynthétiques considérés comme les ancêtres des plastes des végétaux eucaryotes. Présentes dans différents types d’habitats terrestres ou aquatiques, elles peuvent produire des composés bioactifs d’intérêt pour des industries pharmaceutiques, ou encore des toxines présentant un risque potentiel pour la santé humaine et animale. Il est donc important de pouvoir caractériser différentes espèces et différents génotypes de ces organismes de manière indubitable pour pouvoir détecter leur présence/absence, évaluer leur rôle au sein de leur écosystème, ainsi que l’influence des facteurs environnementaux sur leur cycle de vie. La caractérisation taxonomique des cyanobactéries a longtemps été réalisée par l’étude des critères morphologiques. Cependant cette approche s’avère délicate quand ces critères peuvent être altérés par la mise en culture. De plus, cette approche requiert une expérience importante et ne permet pas de différencier des génotypes toxiques. La séquence du gène codant pour l’ARN ribosomique 16S est utilisée pour différencier les cyanobactéries jusqu’au genre et parfois jusqu’à l’espèce. Mais, dans certains cas, elle ne coïncide ni avec la description morphologique spécifique, ni avec la production de certaines toxines. Dans le cadre de cette étude, nous proposons d’évaluer l’information taxonomique et génotypique de marqueurs génétiques singuliers présents dans les génomes de cyanobactéries séquencés d la base de données Genbank. Les distances génétiques entre les parties de 32 génomes suffisamment conservées pour être alignées ont été mesurées. Ces distances ont ensuite été comparées par régression linéaire aux distances génétiques de différents loci précis (ARNr 16S, kaiC, lexA, rpoC1, recA). Les résultats préliminaires montrent un R2 de 0,9141 pour la régression linéaire entre les distances génétiques du gène recA et des régions conservées des génomes séquencés appartenant au genre Prochlorococcus. Ce résultat suggère que recA serait potentiellement un bon marqueur pour génotyper des organismes du genre Prochlorococcus. [less ▲]

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See detailCarbonyl fluoride (COF2) vertical information above Jungfraujoch by FTIR and multi-spectra fitting: error budget and comparisons with KASIMA 3-D CTM model calculations
Duchatelet, Pierre ULg; Mahieu, Emmanuel ULg; Ruhnke, Roland et al

Poster (2008, April)

The major sources of fluorine in the stratosphere are CFC-11 and CFC-12. During the decomposition process, fluorine is first present in the form of carbonyl compounds (e.g. carbonyl fluoride (COF2 ... [more ▼]

The major sources of fluorine in the stratosphere are CFC-11 and CFC-12. During the decomposition process, fluorine is first present in the form of carbonyl compounds (e.g. carbonyl fluoride (COF2)). Given its long life time, COF2 is the second most abundant stratospheric fluorine reservoir. Earliest, COF2 vertical distributions were derived from occultation measurements performed by the ATMOS instrument during the SPACELAB-3 Space Shuttle mission in 1985. The Canadian FTIR spectrometer ACE-FTS, onboard the SCISAT-1 satellite, is the first instrument since the last ATMOS flight in 1994, to record COF2 vertical profiles from space. Four years of ACE-FTS occultation measurements are presently available. Several COF2 IR absorption lines located either in the so-called InSb (1-5 µm) and MCT (2-16 µm) spectral ranges can be used to determine its total column from ground-based high-resolution FTIR observations. In addition, we have proposed, during the EGU General Assembly 2007, an original retrieval procedure that allows us to derive information on the COF2 vertical distribution, between 17 and 30 km, from ground-based FTIR spectra recorded at Jungfraujoch. This “multi-microwindows multi-spectra” approach, using the SFIT-2 v3.91 algorithm, has been completely characterized in terms of fitting strategy and information content. Some examples of COF2 vertical profiles inversions were also presented. This year, we will complete the characterization of the multi-microwindows multi-spectra fitting procedure by exposing the full error budget affecting our COF2 partial and total columns products. Also, comparisons with COF2 model runs generated by the 3D CTM KASIMA (KArlsruhe SImulation model of the Middle Atmosphere) will be presented and critically discussed. [less ▲]

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See detailObservations of halogens, CO, CH4, and H2CO at Ile de La Réunion from ground-based FTIR and MAXDOAS campaign measurements
De Mazière, Martine; Vigouroux, Corinne; Hendrick, François et al

Poster (2008, April)

Ile de La Réunion is a complementary site in the Network for the Detection of Atmospheric Composition Change (NDACC), situated in the southern tropics, at 21°S, 55°E. In support of a better understanding ... [more ▼]

Ile de La Réunion is a complementary site in the Network for the Detection of Atmospheric Composition Change (NDACC), situated in the southern tropics, at 21°S, 55°E. In support of a better understanding of atmospheric chemistry and physics above tropical regions, we have implemented new ground-based MAX-DOAS (multi-axis DOAS) and FTIR (Fourier transform infrared) observations at this site, on a campaign basis since 2002. At present, we have data from 2002 (month of October), 2004 (August to October) and 2007 (end of May until the end of October). Additional campaigns are planned until the availability of a new infrastructure for permanent observations at the Maido (~ 2000 masl), around 2010. Here, we report on the available time series for a number of tropospheric species (CO, H2CO and CH4,) and stratospheric halogen species, comparisons between MAX-DOAS and FTIR data of H2CO, and comparisons of ground-based and satellite data. [less ▲]

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See detailMeasuring CO2 in sea ice: caveats and improvements
Geilfus, Nicolas-Xavier ULg; Delille, Bruno ULg; Verbeke, Véronique et al

Poster (2008, April)

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See detailCerebral correlates of False Memories after Sleep and Sleep Deprivation
Darsaud, A.; Dehon, Hedwige ULg; Sterpenich, Virginie ULg et al

Poster (2008, April)

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See detailOxygen, a tool for assessing ocean tracer transport models
Mouchet, Anne ULg

Poster (2008, April)

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See detailSea ice pCO2 dynamics and related air-ice CO2 fluxes during a flood-freeze cycle (Bellingshausen Sea, Antarctica)
Geilfus, Nicolas-Xavier ULg; Tison, Jean Louis; Carnat, Gauthier et al

Poster (2008, April)

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See detailImplementation of hydrostatic constraint in the software DIVA: Theory and applications
Troupin, Charles ULg; Ouberdous, Mohamed ULg; Rixen, Michel et al

Poster (2008, March 31)

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See detailStudy of the role of Sprouty1 in the regulation of angiogenesis
Sabatel, Céline; Tabruyn, Sébastien ULg; Cornet, Anne et al

Poster (2008, March 30)

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See detailApplication of a chemoselective ligation strategy to the M1 factor of virginiamycin
Nott, Katherine ULg; Tatibouët, Arnaud; Rollin, Patrick et al

Poster (2008, March 26)

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See detailOptoacoustic specific detection of prostate cancer using functionalized gold nanorods
Schol, Daureen ULg; Fleron, Maximilien ULg; Greisch, Jean-François et al

Poster (2008, March 12)

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See detailSIGHTING FREQUENCY AND PHOTO-IDENTIFICATION OF BOTTLENOSE DOLPHINS (TURSIOPS TRUNCATUS) ALONG THE COAST OF BAHÍA SAN ANTONIO, PATAGONIA, ARGENTINA
Holsbeek, Ludo; Cammareri, Alejandro; Failla, Mauricio et al

Poster (2008, March 10)

The sighting frequency (SF), site fidelity, home-range and group composition of bottlenose dolphins (Tursiops truncatus) were assessed during a photo-identification study in 2006-2007. This study aims to ... [more ▼]

The sighting frequency (SF), site fidelity, home-range and group composition of bottlenose dolphins (Tursiops truncatus) were assessed during a photo-identification study in 2006-2007. This study aims to provide first data on this unknown austral bottlenose dolphin population to answer to their increasing conservation needs. Data and pictures were collected during 132 surveys with an average observation effort of 2.8h (SD=1.6) per survey. All clear pictures were analysed using the automatic identification systems FinEx and FinMatch (EuroPhlukes Initiative). The SF was obtained dividing the number of sightings by the amount of effort. Site-fidelity was estimated using the Capture Mark Recapture method and the degree of residency by the re-identification frequency following Culloch (2004). In total, 377.4h were spent searching for bottlenose dolphins of which 57.3h were spent in the presence of 126 dolphin groups divided in 105 sightings. The SF was 0.28/h with and average duration of 43.2min/sighting (SD=0.76). A total of 43 dolphins were classified into an identification catalogue. These recognizable dolphins were re-identified up to 10 days with 44% (n=43) showing a degree of residency (resighting frequency (RF)¿4). The average group size was 6.1 (SD=7.9) ranging from one to fifty animals. Group size increased significantly with the presence of calves and with the distance from the coast. Nevertheless, all dolphins were seen in inshore waters ¿20m deep. The most frequent group formation was loose (34.5%), variable (29.8%), disperse (20.2%) and tight (15.5%). At least 6 identified dolphins, including one mother with her calf, were photographed in the mouth of the Rio Negro 250km from the study area indicating that their home-range is far from limited to Bahia de San Antonio. Data indicate that it concerns a resident but yet unknown population of bottlenose dolphins with a high commercial potential and an urgent need of conservation measurements. [less ▲]

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See detailExtraction of ferulic acid, a natural antioxidant, for potential agro-industrial applications.
Barberousse, Hélène; Roiseux, Olivier; Deroanne, Claude et al

Poster (2008, March 05)

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See detailA structured expert judgement study on Salmonella spp. in pork: analyses of different weighting schemes.
Boone, Idesbald; Van der Stede, Y.; Bollaerts, K. et al

Poster (2008, March)

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See detailFrom Bouncing to boxing
Terwagne, Denis ULg; Gilet, Tristan ULg; Vandewalle, Nicolas ULg et al

Poster (2008, March)

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See detailNorovirus bovins isolés en Belgique en 2007 et investigation de leur potentiel zoonotique par l’étude des interactions virus-celllules
Mauroy, Axel ULg; Scipioni, Alexandra; Mathijs, Elisabeth et al

Poster (2008, March)

Appartenant à la famille des Caliciviridae, les norovirus sont des virus non enveloppés. Leur génome est composé d’un ARN monocaténaire de polarité positive d’approximativement 7,5 kb. Trois cadres ... [more ▼]

Appartenant à la famille des Caliciviridae, les norovirus sont des virus non enveloppés. Leur génome est composé d’un ARN monocaténaire de polarité positive d’approximativement 7,5 kb. Trois cadres ouverts de lecture (ORFs) y sont décrits et l’ORF 2 code pour l’unique protéine composant leur capside. Les norovirus infectent l’homme et les animaux (bovins, porcins, murins). Chez l’homme, ils sont des agents majeurs de gastroentérite sporadique ou épidémique d’origine souvent alimentaire. Chez le bovin, ils seraient également les agents d’entérite bénigne bien qu’à l’heure actuelle aucune épidémie à norovirus n’ait été décrite chez cette espèce. La voie d’infection des norovirus est habituellement oro-fécale, ils sont très résistants dans l’environnement et une infection peut survenir même avec une très faible dose infectieuse. Les norovirus humains et animaux sont relativement proches génétiquement et coexistent parfois de manière très étroite dans nos pays d’Europe du nord. Il est donc logique d’envisager le risque zoonotique lié aux norovirus animaux et plus particulièrement celui lié aux norovirus bovins. Ces derniers sont considérés comme endémiques dans des pays proches de la Belgique et une forte séroprévalence apparente a été montrée dans ce pays. Ce travail avait pour but l’étude moléculaire des souches de norovirus bovins ayant circulé au cours de l’année 2007. L’investigation préliminaire de leur potentiel zoonotique a également été étudiée au travers des interactions virus-cellules. Une banque d’échantillons de matières fécales bovines en provenance d’un laboratoire d’analyse et de diagnostic vétérinaire installé en Région Wallonne (ARSIA) a été constituée tout au long de l’année. Un diagnostic rapide par RT PCR a été effectué pour détecter des séquences de norovirus bovins des deux génotypes décrits actuellement. Les couples d’amorces utilisés, JV12-13, BEC et CBECu s’hybridaient dans les régions codant pour la polymérase virale et dans le début de l’ORF2, régions assez conservées. Ces séquences ont été analysées comparativement à celles isolées dans les années précédentes. Parallèlement, des pseudoparticules d’une souche de norovirus bovin (B309) et d’une souche de norovirus humain (HV) ont été produites comme décrit précédemment avec de légères modifications. Leur concentration protéique a été obtenue par BCA et des lapins ont été immunisés avec ces antigènes. Trois injections de 25 µg d’antigènes dilués dans du PBS et complétées avec les adjuvants complet et incomplet de Freund ont été réalisées. La production d’un sérum hyperimmun dirigé contre la protéine de capside des norovirus a été contrôlée par ELISA. Les pseudoparticules ont ensuite été utilisées pour des études de liaisons sur différents types cellulaires dont les Caco2, cellules connues pour exprimer à leur surface des oligosaccharides proches de ceux des systèmes ABO et de Lewis, ces oligosaccharides étant impliqués comme récepteurs cellulaires pour de nombreux norovirus humains. L’attachement des pseudoparticules a été mis en évidence par immunofluorescence indirecte en utilisant les sérums polyclonaux et un anticorps secondaire anti-lapin couplé à l’Alexa fluor 488. Des séquences de norovirus bovins ont pu être identifiées dans les prélèvements de matières fécales bovines tout au long de la période de constitution de la banque, ces séquences étant proches de celles des norovirus bovins de génotype 2. Une prévalence apparente dans les cheptels bovins de Wallonie a put être déterminée. Au cours de premiers tests réalisés, si les pseudoparticules de HV se sont liées aux cellules Caco2, aucun attachement des pseudoparticules du norovirus bovin à ces mêmes cellules n’a pu être démontré. Nous avons donc montré que les norovirus sont largement répandus en Belgique et leur diagnostic tout au long de la période d’échantillonnage prouve un certain caractère endémique de ces virus en Belgique ; cette constatation rejoignant celles en provenance de pays proches (Grande Bretagne, Allemagne) et de séroprévalence. De ce fait, ils pourraient constituer un risque zoonotique, risque qui pourrait être cependant pondéré par les études préliminaires d’interaction virus-cellules. Des études plus approfondies ont besoin d’être conduites à ce sujet. [less ▲]

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See detailOptimization of the Production of the Amyloidogenic Variants of Human Lysozyme
Menzer, Linda ULg; Tocquin, Pierre ULg; Dony, Nicolas et al

Poster (2008, February 16)

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See detailREconstruction of COLOUR scenes
Sirjacobs, Damien ULg; Alvera Azcarate, Aïda ULg; Barth, Alexander ULg et al

Poster (2008, February 12)

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Peer Reviewed
See detailTalking while walking in multiple sclerosis: Increased difficulty of walking abilities, deficit of working memory, or both?
DELRUE, Gaël ULg; Gryglewicz, Céline; Leonard, Yves et al

Poster (2008, February 02)

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See detailInventaire des dégâts d'écorcement en peuplements résineux
Gheysen, Thibaut ULg; Lejeune, Philippe ULg; Arnal, Delphine et al

Poster (2008, January 25)

Une gestion des populations de cervidés en harmonie avec la forêt et les multiples fonctions qu’elle est aujourd’hui amenée à remplir nécessite notamment de pouvoir apprécier de manière objective et ... [more ▼]

Une gestion des populations de cervidés en harmonie avec la forêt et les multiples fonctions qu’elle est aujourd’hui amenée à remplir nécessite notamment de pouvoir apprécier de manière objective et précise la pression exercée par la grande faune sur les zones davantage réservées à la production ligneuse. Les recensements par observations directes constituent souvent la principale source d’informations à partir desquelles il est habituel d’estimer les populations de cervidés et d’établir les plans de gestion de ces populations. Ces méthodes présentent cependant le double désavantage d’être coûteuses en moyens humains et de produire des résultats dont la précision et l’objectivité laissent parfois à désirer. Aussi, certains auteurs préconisent-ils de remplacer ou, à tout le moins, de compléter ces recensements par une observation des dégâts frais affectant la végétation ligneuse. [less ▲]

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See detailPolar lipids analysis from the MFGM by SPE and HPLC-ELSD
Bodson, Pascal ULg; Danthine, Sabine ULg; Blecker, Christophe ULg et al

Poster (2008, January 23)

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See detailOptiVal: Vers de nouveaux outils de gestion pour les éleveurs laitiers wallons, Prédire la réussite à l’insémination
Laloux, Laurent; Bastin, Catherine ULg; Glorieux, Géry et al

Poster (2008, January 23)

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See detailLa membrane du globule gras du lait (MFGM) : une composition et une structure originale.
Bodson, Pascal; Vanderghem, Caroline ULg; Danthine, Sabine ULg et al

Poster (2008, January 23)

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Peer Reviewed
See detailEffects of intravenous lidocaïne in healthy adult horses.
Amory, Hélène ULg; Lopez Arevaldo, Diégo; Maes, An et al

Poster (2008, January)

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See detailSoil physical fertility: thesis project for water-soil-plant model improvement
Beckers, Eléonore ULg; Degre, Aurore ULg

Poster (2008, January)

Quantities of nutrients like N, P and K in the soil are generally assumed to be the more important parameters of its fertility. The physical fertility of the soil such as water holding capacity, hydraulic ... [more ▼]

Quantities of nutrients like N, P and K in the soil are generally assumed to be the more important parameters of its fertility. The physical fertility of the soil such as water holding capacity, hydraulic conductivity…is often ignored. The present thesis project purposes the development of a physically-based model linking agricultural practices and changes on soil physical properties. [less ▲]

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See detailIsolation of novel hydrolytic genes from an Antarctic metagenomic library
Pipers, D.; Berlemont, R.; Power, P. et al

Poster (2008)

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See detailInfluence of natural and synthetic peptides on biomimetic membranes
Kouzayha, Achraf; Wattraint Olivier; Nasir, Mehmet Nail ULg et al

Poster (2008)

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See detailUse of the daily differencing approach to evaluate uncertainties affecting eddy covariance measurements
Laffineur, Quentin ULg; Aubinet, Marc ULg

Poster (2008)

The eddy covariance technique is recognised to be the most adapted micrometeorological method to study the exchange processes between terrestrial ecosystems and the atmosphere. Like all other methods, it ... [more ▼]

The eddy covariance technique is recognised to be the most adapted micrometeorological method to study the exchange processes between terrestrial ecosystems and the atmosphere. Like all other methods, it is submitted to systematic and random measurement errors. A thorough analysis of these errors is necessary in order to set the limits of validity of the method and to quantify the uncertainty that affects net carbon exchange computed with this method. In this presentation, we’ll concentrate on the random errors using the dailydifferencing approach (DDA) developed by Hollinger and Richardson (2005). The interest of this approach is that it requests only single tower measurements and is thus applicable to all flux tower sites. In this approach, uncertainties are estimated by comparing flux values taken at two successive days at the same hour and under similar meteorological conditions. The analysis may be applied to sensible heat, latent heat and CO2 flux densities. It was applied here to the eddy-covariance data from the Vielsalm mixed forest site (10 years of data) and from the Lonzée agricultural site (4 years of data). Both sites are situated in Belgium and are part of the Carboeurope IP network. The study is developed in the frame of the European IMECC project. The absolute and relative random error was quantified for both sites. Their daily evolution and their dependencies on different climate conditions (magnitude of the flux, PPFD, Rnet, wind velocity, wind direction, clarity index) were analysed. For both sites, the absolute random error increases linearly with the absolute value of flux. This is the principal factor controlling the random error. More particularly the response of the random error to wind velocity was analysed. For the CO2 flux, the absolute random error decreases with increasing wind speed. This effect is more important for the agricultural site than for the forest site. The behaviour of the relative random error with wind speed is more contrasted: it generally decreases with increasing wind speed at low velocities but, for some directions may increase with wind speed at high velocities. In addition, the random error was found very dependent on wind direction at the forest site probably as a result of site heterogeneity. [less ▲]

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See detailDosage des pepsinogènes bovins A et C par RIA
Terroni, D.; Melo de Sousa, Noelita ULg; Michiels, J. A. et al

Poster (2008)

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See detailHeterogeneity of zooxanthellae density in the coral Acropora globiceps around Moorea (French Polynesia)
Ladrière, Ophélie ULg; van Lierde, Elodie; Penin, Lucie et al

Poster (2008)

Understanding the natural heterogeneity of coral zooxanthellae density appears as very important to understand variations in coral survival due to bleaching (the loss of these micro-algal symbionts ... [more ▼]

Understanding the natural heterogeneity of coral zooxanthellae density appears as very important to understand variations in coral survival due to bleaching (the loss of these micro-algal symbionts). Heterogeneity of coral bleaching at different observation scales (within a colony, among neighbouring colonies of the same species or on a wider scale) remains largely misunderstood. The present work explores intracolonial, spatial and temporal variations of zooxanthellae density in the coral Acropora globiceps Dana 1846, over a period of three months on the forereef of Moorea, French Polynesia. In our study, intracolonial zooxanthellae densities did not vary significantly. However, zooxanthellae densities differed slightly between inner and outer branches but this trend was not significant at 6, 12 and 18 meters depth. On a wider scale, zooxanthellae densities also vary spatially : a positive correlation was observed between depth and symbiont density (density increases when light intensity decreases, so according to depth). Moreover, the location of colonies exposed to different hydrodynamical conditions was not a parameter controling the concentration of zooxanthellae. Finally, the temporal variation of zooxanthellae densities did not show significant variations even if it decreased slightly over the study period. The results of this study highlight the importance of accounting for variations within and among colonies to determine zooxanthellae densities and to assess the evolution of zooxanthellae populations. [less ▲]

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See detailE. coli HU dimers dynamics studied by NMR
Augustyniak, R.; Damblon, Christian ULg; Castaing, B.

Poster (2008)

HU is a 2x90 residues, dimeric bacterial histone-like protein involved in chromosome compaction and other DNA-related processes. E. coli HU proteins are composed of three forms: two homodimers α2 and β2 ... [more ▼]

HU is a 2x90 residues, dimeric bacterial histone-like protein involved in chromosome compaction and other DNA-related processes. E. coli HU proteins are composed of three forms: two homodimers α2 and β2 (α and β sharing 70% of sequence identity) and one heterodimer (αβ). Dimeric forms relative abundance varies during cell growth and in response to environmental changes, suggesting that each dimer plays different physiological roles. Unlike other HU proteins which melt through a single step (N2<=>2D), E. coli dimers melt according to a two-step mechanism (N2<=>I2<=>2D). The native dimer, N2, melts partially into a dimeric intermediate, I2, which in turn yields the unfolded monomers, D. Circular Dichroism studies indicate that the intermediate, I2, corresponds to an HU dimer having partly lost its a-helices. Here we compared dynamic properties and structural features of E. Coli HU dimers at different temperatures in order to determine the secondary strutural elements still present in the I2 intermediate. Isotopically labelled proteins (15N, 13C) have been used to perform backbone assignment for all dimeric forms using sequential triple-resonance experiments. 15N-HSQC at different temperatures (288-323K) were recorded .Intensity variations and chemical shift perturbations allow the identification of the secondary element lost in the intermediate I2 confirming the high dynamics of arms responsible for protein-DNA interactions and the thermal stability of HTH motif in all dimeric forms. [less ▲]

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See detailOptimization of a drug-cyclodextrin complexation reaction by a static supercritical carbon dioxide process
Brion, Michael; Nizet, Dominique; Evrard, Brigitte ULg

Poster (2008)

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See detailContrastief taalonderzoek geïntegreerd aanpakken: hoe en waarom?
Rasier, Laurent ULg; Hiligsmann, Philippe; Baelen, Mélanie et al

Poster (2008)

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See detailMECHANICAL PRETREATMENT EFFECT ON CELLULOSE ENZYMATICAL HYDROLYSIS
Jacquet, Nicolas ULg; Paquot, Michel ULg

Poster (2008)

Lignocellulosic materials are very interesting for the production of bioethanol after chemical or enzymatical hydrolysis of cellulose. To optimise production rate and increase the output of these ... [more ▼]

Lignocellulosic materials are very interesting for the production of bioethanol after chemical or enzymatical hydrolysis of cellulose. To optimise production rate and increase the output of these processes, different kinds of pre-treatments (grinding, steam explosion…) are often needed for opening the fibrous structure of the cellulose and increase the specific surface of the materials. The present study is focused on two models of “pure” cellulose (cellulose C200 microtechnik, cellulose FD100 FMC biopolymer) in order to analyse the effects of mechanical pretreatments on the kinetics and yields of hydrolysis. The cristallinity index differentiates the two celluloses. Hydrolysis is realized by a cellulase mix (cellulase Trichoderma reesei C2730, Novozyme; cellobiase Aspergillus niger C6105). Results present the influence of homogenisation pretreatment and analyse differents parameters of this technology. [less ▲]

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See detailInter-annual variability of the carbon dioxide oceanic sink south of Tasmania
Borges, Alberto ULg; Tilbrook, B.; Metzl, N. et al

Poster (2008)

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See detailSecreted subtilisins of Microsporum canis are involved in adhesion of arthroconidia to feline corneocytes
Mathy, Anne ULg; Tabart, Jérémy; Mignon, Bernard ULg et al

Poster (2008)

Microsporum canis is a pathogenic fungus that causes a superficial skin infection called dermatophytosis mainly in cats, dogs and humans. Like other dermatophytoses, the physiopathology of this dermatosis ... [more ▼]

Microsporum canis is a pathogenic fungus that causes a superficial skin infection called dermatophytosis mainly in cats, dogs and humans. Like other dermatophytoses, the physiopathology of this dermatosis remains largely unknown. From a fungal perspective, the infection process can be divided in three steps: adhesion of M. canis arthroconidia to corneocytes, conidial germination, and fungal invasion of the keratin network. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. However, several previously characterized secreted fungal endoproteases like subtilisins (Sub), including the keratinolytic protease Sub3, are secreted in vivo and could be involved in the first pathogenic steps. The objective of this study were (1) to develop an in vitro model to study M. canis adherence to feline corneocytes and (2) to assess whether the Sub are involved in fungal adhesion. An arthroconidial suspension was spread over the surface of reconstituted feline epidermis (RFE). Co-cultures were incubated for varying lengths of time and adherent conidia were labelled using Calcofluor white and counted. In subsequent assays arthroconidia were exposed to the serine protease inhibitor chymostatin or a mixture of two anti-Sub3 monoclonal antibodies (Mabs) one hour prior to the adherence assay. In our model, adherence of M. canis arthroconidia to RFE is time-dependent, beginning within two hours and still increasing after six hours. Chymostatin and Mabs inhibit M. canis adherence to RFE by 53 and 23 % respectively, which suggests that subtilisins and particularly Sub3, are fungal virulence factors involved in the adherence process. [less ▲]

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See detailFunctional properties of phospholipids and proteins from rapeseed as native tensioactives
Vaca Medina, Guadalupe; Mouloungui, Zéphirin; Deleu, Magali ULg et al

Poster (2008)

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See detailMechanisms of ATM regulation by TGF-beta
Paupert, Jenny ULg; Barcellos-Hoff, Mary-Helen

Poster (2008)

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See detailUse of supercritical fluids technology (PGSS) for the production of betametazone loaded solid lipid microparticles
Nizet, Dominique; Jaspart, Séverine ULg; Brion, Michael et al

Poster (2008)

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See detailProtein synthesis and folding is a rate limiting step for bacterial growth at low temperatures.
Piette, Florence; D'Amico, Salvino; Leprince, Pierre ULg et al

Poster (2008)

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See detailCompositional protein analysis of HDL by SELDI-TOF MS during experimental endotoxemia
Levels, Johannes HM; Marée, Raphaël ULg; Geurts, Pierre ULg et al

Poster (2008)

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See detailInfluence of nitrogen fertilizer treatments on soft wheat starch characteristics
Massaux, Carine; Sindic, Marianne ULg; Lenartz, Jonathan et al

Poster (2008)

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See detailFra-1 and its associated transcriptome are central determinants of human breast cancer metastasis
Desmet, Christophe ULg; Prieur, A; Reyal, F et al

Poster (2008)

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See detailNew insights in auditory organ development: the inner pillar cell goes it own way
Thelen, Nicolas; Malgrange, B; Thiry, Marc ULg

Poster (2008)

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