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See detailEstimation of (co)variance components across breeds by a test-day model adapted to New Zealand dairy cattle.
Vanderick, Sylvie ULg; Harris, Bevin; Mayeres, Patrick et al

Poster (2005)

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See detailLinearity and stationarity of the pharmacokinetics of tiludronate in horses
Delguste, Catherine ULg; Doucet, Michèle; Amory, Hélène ULg et al

Poster (2005)

Two studies were conducted in order to assess the linearity and stationarity of the pharmacokinetics of tiludronate in horses. For each study, plasma concentrations of tiludronate were measured by HPLC-UV ... [more ▼]

Two studies were conducted in order to assess the linearity and stationarity of the pharmacokinetics of tiludronate in horses. For each study, plasma concentrations of tiludronate were measured by HPLC-UV at regular intervals and noncompartmental analysis was performed. First, single intravenous administrations of 0.05 (group 1, n = 5), 0.1 (group 2, n = 5) and 0.2 mg/kg (group 3, n = 5) were performed in healthy male adult horses. A linear relationship between groups was found for C5min (C5min = 0.0726 + 11.8589 * dose) as well as for AUC0-inf (AUC0-inf = -0.632561 + 30.9649 * dose). Clearance ranged from 0.033 to 0.044 L/h/kg and was not significantly different between groups. MRT (h) were 3.96, 4.95 and 9.40 and VDss (L/kg) were 0.16, 0.19 and 0.28 for the 0.05, 0.1 and 0.2 mg/kg doses respectively. Second, 10 daily intravenous administrations at a dosage rate of 0.1 mg/kg were administered to 4 healthy horses. Plasma tiludronate concentrations were measured after the 1st and 10th dose as well as 8 hours after each dose. The mean plasma accumulation ratio between the 1st and the 10th dose was 1.31 ± 0.48. Medians of the tiludronate concentration values measured in plasma 8 hours post-dose were not statistically different between doses. Maximum and minimum concentrations of tiludronate (mg/L) following the last dose were 0.91 and 0.04 respectively. It was concluded that the pharmacokinetics of tiludronate in horses are linear from 0.05 to 0.2 mg/kg IV and stationary after 10 IV administrations of 0.1 mg/kg. [less ▲]

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See detailMercury, polychlorobiphenyls and stable isotopes in the blood of harbour seals (Phoca vitulina) from the southern North Sea
Das, Krishna ULg; Lepoint, Gilles ULg; Fonfara, Sonja et al

Poster (2005)

The harbor seal (Phoca vitulina) is one of the most widely distributed seal species and the North Sea contains around 10 % of the world population. The harbour seal population in the North Sea was ... [more ▼]

The harbor seal (Phoca vitulina) is one of the most widely distributed seal species and the North Sea contains around 10 % of the world population. The harbour seal population in the North Sea was estimated at 36 000 individuals between 1994 and 1996). However, recurrent Phocine Distemper Virus (PDV) epizooties have affected the North Sea seal population. Recently, 21 500 harbor seals were killed by PDV in the North Sea and adjacent waters in 2002. Some intriguing questions about the interaction between PDV and immunotoxic contaminants, such as mercury (Hg) and polychlorinated biphenyls (PCBs) remain unanswered. In this framework, circulating levels of Hg and PCBs (PCBs 28, 52, 101, 118, 138, 153, 180) were measured in the blood of 24 harbour seals captured on a sandbank between 2001 and 2004 (Schleswig-Holstein, Germany). As pollutant level may be linked to the trophic position in the food web, carbon and nitrogen isotopic ratios (δ13C and δ15N) were measured in clotted blood cells. The average concentration of mercury in these living seals did not differ significantly from those observed previously in stranded harbour seals (94 ± 41 vs 146 ± 71 µg.l-1 respectively). Mean blood concentrations of total PCBs (∑PCBs) were 11 ng/ml. CB 153 clearly dominated the mix (45%) followed by PCB 138 (31%). The average isotopic composition measured in the blood cells was –15.6 ± 0.3 0/00 and 18.7 ± 0.6 0/00 for δ13C and δ15N respectively, similar to that obtained previously in muscle of stranded individuals, confirming the high position of the harbour seal in the North Sea trophic chain. Further investigations are obviously needed on a larger sampling but our preliminary results suggest that blood is an interesting substrate for both trophic and pollutant long-term monitoring of the harbour seal in the North Sea. [less ▲]

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See detailPhylogeography of Harbour Porpoise (Phocoena phocoena) in the Eastern North Atlantic and in the Black Sea Explored by the Analyses of Nuclear and Mitochondrial DNA
Fontaine, Michaël; Baird, C.; Stuart, J. E. et al

Poster (2005)

Study of the genetic population structure and the demographic history of the harbour porpoise (Phocoena phocoena) has been nearly comprehensive throughout its distribution in North Atlantic, most studies ... [more ▼]

Study of the genetic population structure and the demographic history of the harbour porpoise (Phocoena phocoena) has been nearly comprehensive throughout its distribution in North Atlantic, most studies using the mitochondrial control region as a genetic marker. Although these studies have shown population structure in some parts of the North Atlantic, mitochondrial DNA is a single, maternally inherited locus and therefore insufficient to fully characterize population structure and history. Polymorphism at 11 microsatellite loci was analyzed in harbour porpoises collected throughout the range of the species in the Central and Eastern North Atlantic from the Iberian peninsula northward to Arctic waters (Portugal, Spain, bay of Biscay, Irish waters, English Channel, the southern bay of the North Sea, Norway, Faroe Islands, and Iceland) and also along the coasts of the Black Sea (Turkey, Ukraine, Bulgaria and Georgia). Multilocus tests for allele frequency differences and population structure estimates indicate complete genetic isolation between Atlantic and Black Sea porpoises. No fine population structure was observed within the Black Sea, and this population displayed a low genetic diversity compared to those of Atlantic. These results can be interpreted in the light of the demographic history of this relict population and the strong founder effect and bottleneck it may have undergone in its past evolution. In Eastern North Atlantic waters, microsatellite data revealed fine scale partitioning of the genetic variation. These results will be compared to the pattern previously reported based on the analysis of the mtDNA control region, and seem to correlate with variation in oceanographic features. [less ▲]

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See detailEvaluation of food born lactic acid bacteria for their use as protective cultures in food
Dortu, C.; Thonart, Philippe ULg

Poster (2004, December 17)

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See detailDiscovery of non-biodegradable fossil cellulose.
Lechien, V.; Rodriguez, Ch.; Grandjean, Jean ULg et al

Poster (2004, December 17)

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See detailStudy of a novel elicitor produced by Pseudomonas putida BTP1 in the ISR on bean
Jourdan, Emmanuel ULg; Ongena, Marc ULg; Schaffer, M. et al

Poster (2004, December 17)

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See detailSaturn's UV Aurora Imaged with HST during the Cassini Approach to Saturn
Clarke, J. T.; Gérard, Jean-Claude ULg; Grodent, Denis ULg et al

Poster (2004, December 13)

A series of HST STIS UV images of Saturn's aurora were obtained on 13 days in Jan. 2004 as the Cassini spacecraft measured the approaching solar wind properties. Clear general correlations have been found ... [more ▼]

A series of HST STIS UV images of Saturn's aurora were obtained on 13 days in Jan. 2004 as the Cassini spacecraft measured the approaching solar wind properties. Clear general correlations have been found between the auroral power and a) Saturn's kilometric radiation, and b) the solar wind dynamic pressure, but not with the direction of the interplanetary magnetic field. While these general correlations are now well established, a closer examination of the data raises many interesting questions. Saturn's auroral emissions exhibit both local time and co-rotational properties, the auroral oval does not appear centered on the magnetic and rotational pole, the auroral emissions exhibit large and unexpected motions in latitude with time and/or planetary rotation, and the auroral oval does not appear continuous, but broken with longitude. This talk will present a more detailed look at Saturn's aurora from the HST images, with a comparison of auroral emission properties to those at the Earth and Jupiter. [less ▲]

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See detailEvaluation de l’effet d’oubli induit à la récupération dans le vieillissement normal
Hogge, Michaël; Adam, Stéphane ULg; Collette, Fabienne ULg

Poster (2004, December 04)

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See detailStroop interference and negative priming in normal aging
Hogge, Michaël; Adam, Stéphane ULg; Collette, Fabienne ULg

Poster (2004, December 02)

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See detailCharacterization of inulin stored at different relative humidities by MDSC and X-ray diffraction, related to soption isotherms.
Ronkart, Sébastien; Fougnies, C.; Paquot, Michel ULg et al

Poster (2004, December)

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See detailLa carte des territoires paysagers de Wallonie
Droeven, Emilie ULg; Kummert, Magali

Poster (2004, December)

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See detailCharacterization of the tumor micro-environment after administration of glucocorticoids to understand their radiosensitization effect
Crokart; Jordan; Baudelet et al

Poster (2004, November 26)

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See detailExpresión de la proteína p24 del Virus de la Leucosis Bovina en E. coli y su potencial utilización en pruebas de diagnostico
Gutiérrez, G.; Álvarez, I.; Rodriguez, Sabrina ULg et al

Poster (2004, November 14)

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See detailAnálisis cladístico de la correlación entre patogenia y marcadores moleculares en la región 5’ LTR del Virus de la Leucosis Bovina
Rodriguez, Sabrina ULg; Zwerdling, A.; Jones, L.R. et al

Poster (2004, November 14)

ANALISIS CLADISTICO DE LA CORRELACION ENTRE PATOGENIA Y MARCADORES MOLECULARES EN LA REGION 5’ LTR DEL VIRUS DE LA LEUCOSIS BOVINA. SM Rodriguez1; A Zwerdling1; LR Jones1; K Trono1 1Instituto de Virología ... [more ▼]

ANALISIS CLADISTICO DE LA CORRELACION ENTRE PATOGENIA Y MARCADORES MOLECULARES EN LA REGION 5’ LTR DEL VIRUS DE LA LEUCOSIS BOVINA. SM Rodriguez1; A Zwerdling1; LR Jones1; K Trono1 1Instituto de Virología, CICVyA, INTA-Castelar. CC 25 (1712), Castelar, Argentina. El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un miembro de la familia Retroviridae. Esta familia incluye los virus responsables de la leucemia de células T de los humanos (Human T-Cell Lymphotropic Virus-1 y -2) y de los simios (Simian T-lymhotropic Virus). BLV afecta al ganado bovino produciendo en un reducido numero de animales (2-5%) una neoplasia linfoproliferativa de células B de origen clonal conocida como Leucosis Bovina Enzootica (LBE). La mayoría de los individuos infectados con BLV son portadores asintomáticos (AL), cursando la infección sin signos clínicos aparentes. Alrededor del 30% de los animales infectados desarrolla una proliferación benigna policlonal de células B, denominada Linfocitosis Persistente (LP). En nuestro laboratorio hemos secuenciado la zona R-U5 del sector 5' LTR de aislamientos provenientes de 36 animales presentando diferentes formas clínicas. El resultado de estos estudios reveló la presencia de 4 bases covariantes para provirus aislados de linfosarcoma (LS). Estas modificaciones están ausentes en la mayoría (16/22) de los provirus aislados de formas asintomáticas (AL/PL). Si bien esta aparente correlación entre los cambios de nucleótidos y los cuadros clínicos sugiere la presencia de marcadores de patogenia, es necesario analizar dentro de un contexto histórico (cladograma) la aparición independiente de dicho marcador en conjunto con el desarrollo de LS. Para ello, se obtuvieron las secuencias correspondientes a la región genómica codificante env y pX para algunos de estos aislamientos de LS y AL/PL. Las secuencias fueron alineadas mediante el programa ClustalX y los alineamientos fueron utilizados para obtener cladogramas mediante las rutinas de máxima parsimonia del programa PAUP*. El análisis de los árboles obtenidos sugiere que la adquisición del marcador en la región LTR y el desarrollo de LS en diferentes aislamientos serían independientes, apoyando la hipótesis de la existencia de un marcador de patogenia en dicha zona regulatoria. [less ▲]

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See detailCaractérisation de la farnésyle pyrophosphate synthase de pucerons
Beliveau, Catherine; Vandermoten, Sophie ULg; Francis, Frédéric ULg et al

Poster (2004, November)

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See detailcomparison between co-crystallisation properties of POP and OPP : a tool to understand industrial processes
gibon, Véronique; tirtiaux, alain; Danthine, Sabine ULg et al

Poster (2004, November)

Detailed reference viewed: 7 (1 ULg)
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See detailPhysicochemical Characteristics of some ternary Fat Blends
Danthine, Sabine ULg; gibon, véronique; norberg, B. et al

Poster (2004, November)

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See detailCharacterization of Cytotoxic Necrotizing Factor (CNF)-producing Escherichia coli isolated from cattle in Argentina
Mercado, E.C.; Rodriguez, Sabrina ULg; Elizondo, A.M. et al

Poster (2004, October 24)

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See detailVers une analyse de la littérature francophone sur l’intervention en sport
Cloes, Marc ULg; Lenzen, Benoit; Trudel, Pierre

Poster (2004, October 23)

Les auteurs proposent une première analyse de la littérature francophone centrée sur la 'science du coaching'. Il s'agit d'une approche multidimensionnelle permettant d'identifier les principales ... [more ▼]

Les auteurs proposent une première analyse de la littérature francophone centrée sur la 'science du coaching'. Il s'agit d'une approche multidimensionnelle permettant d'identifier les principales caractéristiques des études publiées dans ce domaine de recherche en plein développement. [less ▲]

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See detailSubacute effects of sertraline on attentional and executive functions in major depression
Constant, Eric; Adam, Stéphane ULg; Gillain, B. et al

Poster (2004, October 10)

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See detailPharmacophore generation to design new leads for selective cyclooxygenase-2 inhibition
Michaux, C.; Julemont, F.; De Leval, X. et al

Poster (2004, October 07)

Detailed reference viewed: 8 (0 ULg)
See detailBiocompatible biodegradable polymer microparticles with entrapped peptide for wound treatment
Stashevskaya, K; Markvicheva, E; Dugina, T et al

Poster (2004, October 04)

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See detailTilted peptides in amyloidogenic proteins
Crowet, Jean-Marc ULg; Brasseur, Robert ULg; Lins, Laurence ULg

Poster (2004, October)

Amyloidogenic proteins are of interest in the study of transconformational processes because they undergo a major conformational change leading to amyloid fibrils formation by a self-assembly of their ... [more ▼]

Amyloidogenic proteins are of interest in the study of transconformational processes because they undergo a major conformational change leading to amyloid fibrils formation by a self-assembly of their misfolded form.   Amyloid fibrils are implicated in several diseases such as Alzheimer, Parkinson or Creutzfeldt-Jakob diseases. Fibrils have varying lengths up to few micrometers, they are unbranched and the individual β strands are oriented perpendicular to the long axis of the fibril. Moreover, the conformational changes induce an increase in the β content. However little is known about the mechanisms of transconformation, the subsequent aggregation and the mechanism toxicity of these proteins. Study of these misfolded proteins, and particularly study of their conformation, is difficult due to their high tendency to aggregate in solution.   Regarding the transconformational mechanisms, it’s interesting to study the potential role of tilted peptides because they possess several properties which could be involved in this process. They can adopt  or β conformation depending on the environment. When helical, they can destabilize the hydrophilic/hydrophobic interface of an organised system. They were notably found in the domain undergoing transconformation in the Aβ protein (Alzheimer’s disease) and in PrP (prion diseases). They could have an important role in the first step of the transconformational process ; their destabilizing properties could also be implicated in the toxicity of these proteins. [less ▲]

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See detailDomain oriented model reduction of signal transduction models
Conzelmann, H.; Saez-Rodriguez, J.; Sauter, T. et al

Poster (2004, October)

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See detailExpression of osteopontin and its receptor the alphavbeta3 integrin in the 5TMM myeloma model
CAERS, Jo ULg; De Raeve, Hendrik; Asosingh, Kewal et al

Poster (2004, September 17)

NA

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See detailUtilidad de la prueba de PCR para el diagnóstico rápido de los Herpes Virus Equinos tipo 1 y 4 en forma combinada
Álvarez, I.; Craig, M.I.; Rodriguez, Sabrina ULg et al

Poster (2004, September 15)

UTILIDAD DE LA PRUEBA DE PCR PARA EL DIAGNOSTICO RAPIDO DE LOS HERPES VIRUS EQUINOS TIPO 1 Y 4 EN FORMA COMBINADA Alvarez I.1*,Craig M.I.1, Rodríguez S.1, Barrandeguy M.1 y Trono K.1 1 Instituto de ... [more ▼]

UTILIDAD DE LA PRUEBA DE PCR PARA EL DIAGNOSTICO RAPIDO DE LOS HERPES VIRUS EQUINOS TIPO 1 Y 4 EN FORMA COMBINADA Alvarez I.1*,Craig M.I.1, Rodríguez S.1, Barrandeguy M.1 y Trono K.1 1 Instituto de Virología, CICVyA. INTA. CC25. 1712. Castelar. Argentina *ialvarez@cicv.inta.gov.ar Cuando se utilizan técnicas de diagnóstico virológico basadas en sistemas libre de células como la prueba de PCR, resulta crítico demostrar que los resultados que se obtienen de su aplicación concuerdan con los resultados obtenidos con el aislamiento viral en cultivo de tejidos, ya que se ha demostrado que la detección de ácidos nucleicos en muestras en estudio no siempre tiene una correlación directa con la causa del cuadro clínico observado. El significado que puede atribuirse a los resultados obtenidos por la prueba de PCR dependerá exclusivamente de la concordancia obtenida con la prueba Gold Standard, lo que permitirá definir claramente el contexto de aplicación de la PCR y la interpretación de los resultados. Con el objetivo de disponer de una herramienta de diagnóstico combinada, rápida y sin utilizar cultivos celulares para la detección del herpes virus equino tipo 1 (HVE-1) y del herpes virus equino tipo 4 (HVE-4), causantes del aborto viral y de un cuadro respiratorio en potrillos, se estandarizó la técnica de PCR según fue descripta en Lawrence et a (1994) con algunas modificaciones, y se evaluó su performance al aplicarse para el diagnóstico de los agentes mencionados en muestras de campo procedentes de casos naturales de abortos o cuadros de tos y moco. La prueba utilizada en este trabajo es un ensayo de tipo múltiple que amplifica fragmentos complementarios al gen de la glicoproteína C del HVE-1 y al gen 76 del HVE-4, donde el tamaño diferencial de los fragmentos obtenidos permite distinguir el origen del material amplificado. Se siguieron los tres criterios básicos descriptos por Hiney and Smith (1999) para la validación de una prueba in vitro de amplificación enzimática, es decir, la evaluación de la cantidad de detección (sensibilidad), calidad de detección (especificidad) y confiabilidad de la prueba en estudio, que fueron evaluados en forma secuencial. A partir de las muestras de abortos e hisopados nasales que ingresaron al laboratorio para análisis virológico por aislamiento viral en cultivo de células desde 1996 hasta la actualidad, se seleccionaron y analizaron un total de 166 muestras. De un total de 34 hisopados nasales, 2 resultaron positivos por las 2 pruebas, ninguno de los hisopos negativos por una de las 2 pruebas resultó positivo por la otra, con un 100% de correlación de resultados. Asimismo, de un total de 132 muestras de aborto analizadas, 31 resultaron positivas por las 2 pruebas, ninguno de los abortos negativos por una las 2 pruebas resultó positivo por la otra, con un 100% de correlación de resultados. Se obtuvo una correlación total entre los resultados obtenidos por la prueba de PCR y el diagnóstico de cultivos de tejidos, considerando los 2 virus en cuestión. Para evaluar la especificidad de la técnica utilizada, se realizó la prueba de PCR utilizando como templado material genético procedente de agentes virales relacionados con el mismo cuadro patológico como el Virus de la Influenza Equina (VIE) y el Virus de la Arteritis Viral Equina (AVE). Además se utilizaron dos virus de la misma familia causantes de otros cuadros patológicos, Herpes virus Equino 2 (HVE-2) y Herpes Virus Equino 3 (HVE-3). No se observaron resultados positivos con ninguno de los cuatro templados utilizados y se confirmó que no amplifica fragmentos inespecíficos. La utilización de una determinada técnica para el diagnóstico del Herpes Virus Equino necesita un alto nivel de confiabilidad, ya que los resultados de la metodología usada podrían condicionar las decisiones referidas al manejo de la población equina bajo estudio. Los resultados obtenidos en el presente trabajo permiten otorgar un importante valor diagnóstico a la prueba de PCR para la detección combinada de Herpes Virus Equino 1 y 4 debido a que presenta total concordancia con los resultados obtenidos en el laboratorio utilizando la prueba de diagnostico convencional de aislamiento viral en cultivo de células. Además es importante recalcar que es una prueba rápida donde el resultado puede obtenerse a las 4 horas. Por esto se puede concluir que la PCR múltiple es una herramienta de diagnostico importante que posibilita tomar medidas de contención inmediatas disminuyendo el impacto económico de estas infecciones en la producción equina. Hiney, M.P. and Smith, P.R. (1998). Validation of polymerase chain reaction- based techniques for proxy detection of bacterial fish pathogens: framework, problems and possible solutions for environmental applications. Aquaculture 162:41-68 Lawrence G.L., Gilkerson J., Love D.N., Sabine M., Whalley J.M. (1994). Rapid, single-step differentiation of equid herpesviruses 1 and 4 from clinical material using the polymerase chain reaction and virus-specific primer. Journal of Virological Methods 47:59-72 [less ▲]

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See detailAlkenone carbon isotopes during a bloom of Emiliania huxleyi: Effects of CO2 concentration and production
Benthien, Albert; Riebesell, Ulf; Engel, Anja et al

Poster (2004, September 05)

The carbon isotopic composition of the C37-alkenones has been used in various paleoceanographic studies to estimate the ancient surface water CO2 concentration [CO2aq]. A number of recent culture, field ... [more ▼]

The carbon isotopic composition of the C37-alkenones has been used in various paleoceanographic studies to estimate the ancient surface water CO2 concentration [CO2aq]. A number of recent culture, field and sediment studies, however, indicate that the carbon isotopic fractionation in haptophyte algae is predominantly controlled by physiological processes and environmental factors other than the ambient [CO2aq]. The most prominent factors are algal growth rate, nutrient availability, light intensity, the carbon uptake mechanism, and the carbon source. To what extent these different factors might affect the carbon isotopic signal of alkenones ultimately preserved in the sediment is still under debate. Causes of uncertainty are the individual strenghts and weaknesses of the different methodological approaches. Culture experiments, for example, cannot perfectly recreate the sum of natural growth conditions and physical processes affecting the carbon isotopic signal in the field and its preservation in the sediment. On the other hand, core-top data represent several hundred to a couple of thousand years and therefore only reflect an average. Here, we present the first study testing the effects of [CO2aq] on the alkenone isotopic signal under natural bloom conditions in a semi-closed system. In a series of 9 floating mesocosms in a Norwegian fjord a bloom of Emiliania huxleyi was followed over a three week period. The mesocosms were covered by gas tight tents. The atmospheric and seawater pCO2 were manipulated to achieve different CO2 levels in the tent atmosphere ranging from pre-industrial (190 ppmv) to year 2100 levels (680 ppmv) as predicted by the IPCC’s report. We found that during the exponential growth phase the isotopic fractionation of alkenones decreased by 5 to 7 per mill and reached a plateau during the stationary phase. During the stationary phase the alkenone content per cell increased from 1-2 pg/cell to 6-8 pg/cell. Between the [CO2aq] treatments we observed an alkenone isotopic difference of only 2 per mill. These results indicate that changes in algal physiology and/or environmental conditions occuring during the course of an algal bloom strongly affect alkenone isotope fractionation. This effect overrides a comparatively small variation in the alkenone isotopic signal due to [CO2aq]. Implications for alkenone isotopic fractionation as a paleo-production or paleo-nutrient proxy will be discussed. [less ▲]

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See detailMarcadores genéticos de virulencia en el Virus de la Leucosis Bovina: Un enfoque filogenético.
Rodriguez, Sabrina ULg; Zwerdling, A.; Jones, L.R. et al

Poster (2004, September 02)

Marcadores genéticos de virulencia en el Virus de la Leucosis Bovina. Un enfoque filogenético. Rodriguez, SM1; Zwerdling A1; Jones, LR1; Trono, K1 1Instituto de Virología, CICVyA, INTA-Castelar. CC 25 ... [more ▼]

Marcadores genéticos de virulencia en el Virus de la Leucosis Bovina. Un enfoque filogenético. Rodriguez, SM1; Zwerdling A1; Jones, LR1; Trono, K1 1Instituto de Virología, CICVyA, INTA-Castelar. CC 25 (1712), Castelar. El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un retrovirus oncogénico B-linfocitotrópico perteneciente al genero Deltaretrovirus que afecta al ganado bovino. Junto a los virus responsables de la leucemia de células T de los humanos (Human T-Cell Lymphotropic Virus-1 y Human T-Cell Lymphotropic Virus-2) y de los simios (Simian T-lymhotropic Virus), están clasificados dentro de la familia Retroviridae. Estos virus están asociados con malignidades de células B o T del sistema inmune. La mayoría de los individuos infectados con BLV son portadores asintomáticos en los que la infección pasa clínicamente desapercibida (AL). Cerca del 30% de los animales infectados desarrolla una proliferación benigna policlonal de células B, denominada Linfocitosis Persistente (LP). Sólo un pequeño porcentaje de los animales infectados (1 a 5%) desarrolla linfomas malignos o linfosarcomas (LS) de origen clonal. Realizando comparaciones entre las regiones genómicas 5' Long Terminal Repeats (5' LTR), involucradas en la regulación de la expresión génica viral, observamos la existencia de una correlación entre algunos sitios nucleotídicos y el desarrollo de casos de LS. Si bien esta correlación sugiere la presencia de marcadores de patogenia en la región LTR, es necesario analizar en un contexto histórico (cladograma) la aparición independiente de dicho marcador en conjunto con el desarrollo de LS. Con ese objetivo, se obtuvieron secuencias correspondientes a las regiones genómicas codificantes para las proteínas Env (utilizada en varios trabajos para realizar estudios comparativos) y Px. Las secuencias fueron alineadas mediante el programa ClustalX y los alineamientos fueron utilizados para obtener cladogramas mediante las rutinas de máxima parsimonia del programa PAUP*. El análisis de los árboles obtenidos sugiere que la adquisición del marcador en el sector LTR y el desarrollo de LS en diferentes aislamientos serían independientes, apoyando la hipótesis de la existencia de un marcador de patogenia en dicho sector. Sin embargo, el análisis debe ser ampliado incluyendo más aislamientos del virus y posiblemente otras regiones genómicas, para posibilitar la realización de análisis estadísticos de la correlación y confirmar los resultados del análisis filogenético. Cabe destacar que las topologías obtenidas a partir de las regiones genómicas Env y Px presentan diferencias, lo cual abre la discusión acerca de qué región utilizar para realizar estudios comparativos y filogenéticos para estudiar este virus. Palabras Claves: BLV, LTR, Env, pX, cladograma [less ▲]

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See detailIntrinsic and irradiation-induced tumor selectivity of liposome-based gene therapy targeting Akt activation.
Martinive, Philippe ULg; Sonveaux, Pierre; Brouet, Agnes et al

Poster (2004, September)

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See detailTaxonomical study of lactic acid-producing spore-forming bacteria isolated from soil.
Bayane, A.; Diawara, B.; Roblain, D. et al

Poster (2004, September)

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See detailToxards a SCAR Marine Biodiversity Information Network (SCAR MarBIN)
De Broyer, Claude; Danis, Bruno; Meerhaege, Angelo et al

Poster (2004, September)

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See detailLipids composition of milk fat globule membrane extracted from cream
Bodson, Pascal; Danthine, Sabine ULg; Blecker, Christophe ULg

Poster (2004, September)

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See detailHeating effects on some quality caracteristics of date seed oil.
Besbes, Souhail; Blecker, Christophe ULg; Danthine, Sabine ULg et al

Poster (2004, September)

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See detailPlasma composition in the Jovian magnetosphere
Radioti, Aikaterini ULg; Krupp, N.; Woch, J. et al

Poster (2004, August 03)

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See detailPhase separation during silica gel formation followed by time-resolved SAXS
Gommes, Cédric ULg; Goderis, Bart; Pirard, Jean-Paul ULg et al

Poster (2004, August)

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See detailAnalysis of the volleyball spike: relationships between several parameters and the speed of the ball
Cloes, Marc ULg; Croisier, Jean-Louis ULg; Barsin, M. et al

Poster (2004, August)

In volleyball, the spike's effectiveness is a determining element of the team's success. To perform at a high level, a player must be able to produce spikes characterized by a speed of the ball that ... [more ▼]

In volleyball, the spike's effectiveness is a determining element of the team's success. To perform at a high level, a player must be able to produce spikes characterized by a speed of the ball that increase the defence's uncertainly. A high speed spike needs technical and muscular qualities. ... [less ▲]

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See detailPE teachers’ competencies: which ones are considered as the most important and where are they acquired?
Cloes, Marc ULg; Laraki, Nadine; Piéron, Maurice

Poster (2004, August)

It is well accepted that teacher prepration is a long term process during which a large array of competencies are progressively developed. Since 2001, in the French Community of Belgium, a decree lists ... [more ▼]

It is well accepted that teacher prepration is a long term process during which a large array of competencies are progressively developed. Since 2001, in the French Community of Belgium, a decree lists the competencies that teachers should develop to achieve their role within the educational system. This study aimed to identify competencies taht PE teachers consider as important and to determine, from their point of view, in which context these competencies are developed. [less ▲]

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See detailAnalysis of anthropometric caracteristics and jumping ability in junior top level volleyball athletes. Comparison by role
Ciccarone, G.; Fontani, G.; Albert, Adelin ULg et al

Poster (2004, August)

This study is focused on the comparison of the players characteristics in youth Italian top level volleyball athletes according to the role on the game.

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See detailTowards a SCAR 'Marine Biodiversity Information Network'
De Broyer, Claude; Danis, Bruno; Meerhaeghe, A et al

Poster (2004, July)

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See detailCatalytic applications of transition metal N-heterocyclic carbene complexes bearing nitrogen-containing side-groups
Tudose, Adriana; Sauvage, Xavier; Delfosse, Sébastien et al

Poster (2004, July)

Herein, we report preliminary results in olefin cyclopropanation, olefin metathesis, atom transfer radical reactions (ATRA and ATRP), and in the Suzuki coupling reaction when catalyst systems were ... [more ▼]

Herein, we report preliminary results in olefin cyclopropanation, olefin metathesis, atom transfer radical reactions (ATRA and ATRP), and in the Suzuki coupling reaction when catalyst systems were generated in situ from [RuCl2(p-cymene)]2 or Pd(OAc)2, and an imidazolium salt bearing nitrogen-containing substituents. [less ▲]

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See detailEstrogens and pain : a study in aromatase knock-out mice using the formalin model
Multon, Sylvie ULg; Mosen, Jeanine; Wouters, Murielle ULg et al

Poster (2004, July)

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See detailIntercomparisons of MIPAS operational N2O and HNO3 profiles with ground based FTIR data
Vigouroux, C.; De Mazière, M.; Wood, S. et al

Poster (2004, July)

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See detailGallbladder mucocele: diagnosis, treatment and outcome in 4 dogs
Carofiglio, Francesca; Bongartz, Alexandre; Busoni, Valeria ULg et al

Poster (2004, July)

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See detailDistribution of nerve fibers and prion protein expression in mouse Peyer's patches
Defaweux, Valérie ULg; Dorban, G; Demonceau, C et al

Poster (2004, July)

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See detailRelaxant effects on the rat uterine smooth muscle of putative K+ATP channel openers
Boverie, S.; De Tullio, Pascal ULg; Sebille, S. et al

Poster (2004, July)

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See detailNew analogues of the potassium channel opener cromakalim : effects on rat pancreatic B-cells and rat aorta rings
Sebille, S.; De Tullio, Pascal ULg; Antoine, M. H. et al

Poster (2004, July)

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See detailDirected forgetting in normal aging: the role of processing speed
Hogge, Michaël; Adam, Stéphane ULg; Collette, Fabienne ULg

Poster (2004, June 25)

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See detailLipid and oxylipin profile during storage of potato tubers
Fauconnier, Marie-Laure ULg; Welti, R.; Delaplace, Pierre ULg et al

Poster (2004, June 03)

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See detailLipid and oxylipin profile during storage of potato tubers
Fauconnier, Marie-Laure ULg; Welti, R.; Delaplace, Pierre ULg et al

Poster (2004, June 01)

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See detailTwo-color-sum-frequency generation characterization of a protein monolayer adsorbed on a metallic substrate
Dreesen, Laurent ULg; Sartenaer, Yannick; Humbert, Christophe et al

Poster (2004, June)

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See detailConcentration of Polybrominated Diphenyl Ethers (PBDEs) in House Hold Dust – Inhalation a Potential Route of Human Exposure.
sjodin; Papke; Mc Gahee et al

Poster (2004, June)

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See detailSynthèse et évaluation pharmacologique d’analogues pyridiniques du nimésulide : variation du pont « inter-cycle
Julemont, F.; De Leval, X.; Dogne, J. M. et al

Poster (2004, June)

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See detailIdentification d’un inhibiteur COX-2 sélectif », 18èmes Journées Franco-belges de Pharmacochimie
Michaux, C.; Julemont, F.; De Leval, X. et al

Poster (2004, June)

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See detailPrediction of bone regeneration in an in vivo bone chamber: application of different mathematical models
Geris, Liesbet ULg; Van Oosterwyck, Hans; Gerisch, A. et al

Poster (2004, June)

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See detailPolymer layered silicate/carbon nanotubes nanocomposites: morphological and rheological properties
Peeterbroeck, S.; Alexandre, Michaël ULg; BNagy, J. et al

Poster (2004, May 27)

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See detailDelivery of single polymer chains on a target substrate by AFM mechanochemistry
Cuenot, Stéphane; Jérôme, Christine ULg; Gabriel, Sabine ULg et al

Poster (2004, May 27)

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See detailSynthesis of pyerene containing polymers and noncovalent side-wall functionlization of multi-walled carbon nanotubes
Lou, Xudong; Detrembleur, Christophe ULg; Pagnoulle, Christophe et al

Poster (2004, May 27)

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See detailTwo-color-sum-frequency generation characterization of a protein monolayer adsorbed on a metallic substrate
Dreesen, Laurent ULg; Sartenaer, Yannick; Humbert, Christophe et al

Poster (2004, May 25)

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See detailConstruction of a set of vectors allowing inducible production of siRNA in Schizosaccharomyces pombe.
Stroobants, Aurore ULg; Tafforeau, Lionel; Guiguen, Allan et al

Poster (2004, May 14)

RNA interference (RNAi) is a sequence-specific gene silencing mechanism. It is induced by the formation of dsRNA that are recognised by the Dicer complex and processed into 21-23 long oligonucleotides ... [more ▼]

RNA interference (RNAi) is a sequence-specific gene silencing mechanism. It is induced by the formation of dsRNA that are recognised by the Dicer complex and processed into 21-23 long oligonucleotides called siRNA (short interfering RNA). Subsequently, RISC (RNA-Inducing Silencing Complex) binds siRNA that targets the complex towards its homologous mRNA (DYKXHOORN et al., 2003) which is eventually degraded. In contrast to budding yeast, the entire pathway is conserved in the fission yeast Schizosaccharomyces pombe, making it a valuable organism to both study physiological RNAi and to use it as a inducible gene knock-down tool. In an attempt to apply this method in the fission yeast, we are using three different approaches to produce siRNA. In each case, a vector containing a regulatable promoter activated in presence of tetracycline (tTA') (GOSSEN et al., 1995) is generated and the ura4 marker required for growth on medium lacking uracil serves as reporter. First, a vector expressing the full lenght antisens RNA of ura4 (800 nucleotides) (RAPONI and ARNDT, 2003) is used. Second, we are trying to generate much shorter dsRNA where both strands are linked by either a short hairpin of 25 nucleotides (BRUMMELKAMP et al., 2002) or a longer one of 350 nucleotides (SCHRAMKE and ALLSHIRE, 2003). The ability of these different dsRNA to induce silencing of ura4 will be presented. [less ▲]

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See detailDelayed and reduced coccolithophorid calcification under elevated PCO2
Delille, Bruno ULg; Harlay, Jérôme ULg; Zondervan, Ingrid et al

Poster (2004, May 10)

Numerous experiments to date have demonstrated that elevated PCO2 is detrimental to biogenic calcification rates. However, most of these experiments have been realized in batch or continuous cultures and ... [more ▼]

Numerous experiments to date have demonstrated that elevated PCO2 is detrimental to biogenic calcification rates. However, most of these experiments have been realized in batch or continuous cultures and give little information on the dynamics of calcification in natural conditions. The present work describes the development and decay of a nutrient-induced bloom of the coccolithophorid Emiliania huxleyi in a mesocosm experiment. The monitoring of Dissolved Inorganic Carbon (DIC) and Total Alkalinity (TAlk) within the seawater enclosures allowed us to describe comprehensively day to day dynamics of both calcification and organic carbon production. Three atmospheric PCO2 conditions (glacial, present and next century) were simulated by bubbling CO2 mixtures, while total alkalinity was left to evolve from its present value. No conspicuous change of Net Community Production under elevated PCO2 was recorded while the production of inorganic carbon appeared to be affected in two ways. Firstly, the production rate of inorganic carbon appeared to be lowered by 40% in the next century PCO2 conditions, decreasing concomitantly the calcification to photosynthesis ratio from 0.75 (glacial conditions) to 0.45 (next century conditions). Secondly, the onset of calcification was delayed by 24~48h under elevated PCO2 conditions, reducing the overall length of calcification in the course of the bloom. These two effects would act to reduce the amount of precipitated CaCO3 by coccolithophorids in a High CO2 world [less ▲]

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See detailBiogeochemical implications of calcification and secondary production of a population of the brittle star Acrocnida brachiata in a temperate coastal ecosystem
Harlay, Jérôme ULg; Gentil, Franck; Davoult, Dominique

Poster (2004, May 10)

The production of organic matter and calcium carbonate by a population of the brittle star Acrocnida brachiata (Echinodermata) was calculated using demographic structure, population density, and relations ... [more ▼]

The production of organic matter and calcium carbonate by a population of the brittle star Acrocnida brachiata (Echinodermata) was calculated using demographic structure, population density, and relations between the size and the ash-free dry weight (AFDW) or the calcimass. During a two-year survey in the Bay of Seine (English Channel, France), one of the four major estuaries affecting the biogeochemical cycling of elements in the southern bight of the North Sea, benthic production of this species amounted to 40.3 g.m-2.yr-1 for AFDW and 80.0 g.m-2.yr-1 for CaCO3. Respiration, based on secondary production, was estimated to be 736 mmol CO2.m-2.yr-1. Using the molar ratio () of CO2 released to CaCO3 precipitated, this biogenic precipitation of calcium carbonate would result in an additional release of 617 mmol CO2.m-2.yr-1. The results of the present study demonstrate that marine calcification should be considered as a significant source of CO2 to seawater and thus a potential source of CO2 to the atmosphere, emphasizing the important role of the biomineralization and dissolution in the carbon budget of temperate coastal ecosystems. This additional biogenic source of CO2 could increase in the future in two ways: firstly, as a positive feedback of  to the rising pCO2 and, secondly, as a positive response to the availability of nutrients due to eutrophication. [less ▲]

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See detailRetrieval-induced forgetting in normal aging
Hogge, Michaël; Adam, Stéphane ULg; Collette, Fabienne ULg

Poster (2004, May 05)

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