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See detailA DESIGN SPACE APPROACH TO DEVELOP A GENERIC CE METHOD FOR THE SEPARATION OF 19 ANTIMALARIAL DRUGS
Lamalle, Caroline ULg; Marini Djang'Eing'A, Roland ULg; Debrus, Benjamin ULg et al

Poster (2010, September)

This project consists in analysing different molecules used against malaria by capillary electrophoresis (CE).

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See detailFunctional suppression of the yeast msb3- mutation
Biver, Sophie ULg; Vandenbol, Micheline ULg

Poster (2010, September)

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See detailInvolvement of Placental growth factor in Wallerian degeneration
Chaballe, Linda ULg; Close, Pierre ULg; Sempels, Maxime ULg et al

Poster (2010, September)

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See detailMesure de la sensibilité circadienne à la lumière avec la pupillométrie : données préliminaires
Daneault, V; Hébert, M; Vandewalle, Gilles ULg et al

Poster (2010, September)

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See detailTOLERANCE INTERVALS AS CONTROL CHART: COMPARISON TO CLASSIC SHEWHART X̅-R CONTROL CHART
Marini Djang'Eing'A, Roland ULg; Lambert, Véronique; Denooz, Raphael ULg et al

Poster (2010, September)

Detailed reference viewed: 244 (43 ULg)
See detailNew MALDI-Mass Spectrometry Imaging Procedures to Highlight Potential Proteic And Lipidic Biomarkers in Ovarian Cancer
Mériaux, Céline; Franck, Julien; Longuespée, Rémi ULg et al

Poster (2010, September)

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See detailComparaison d'une méthode "acide" et enzymatique pour le dosage de l'inuline
Thibaudin, L.; Delanaye, Pierre ULg; Maillard, N. et al

Poster (2010, September)

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See detailApplication of experimental design for the HPLC-UV-MS separation of aporphine alkaloids from leaves of Spirospermum Penduliflorum thouars
Rafamantanana, Mamy H.; Debrus, Benjamin ULg; Raoelison, Guy E. et al

Poster (2010, September)

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See detailSleep in Disorders of Consciousness
Schabus, Manuel; Cologan, Victor ULg; Weihart, K et al

Poster (2010, September)

Résultats préliminaires de l'étude du sommeil chez les patients cérébrolésés en état de conscience altéré.

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See detailTransfer of a conventional LC method for the screening of conterfeit antimalarial medicines to UHPLC
Nistor, Iolanda ULg; Lecomte, Frédéric ULg; Mbinze Kindenge, Jérémie et al

Poster (2010, September)

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See detailA new method to enumerate low level of Listeria monocyogenes and Salmonella spp. in meat products
Delhalle, L.; Adolphe, Y; Jasick, A. et al

Poster (2010, September)

Micro-organisms counting techniques are a subject of major concern in the field of food hygiene. The European Community Regulation No 2073/2005 defined a quantitative limit for 100 cfu.g-1 which was is ... [more ▼]

Micro-organisms counting techniques are a subject of major concern in the field of food hygiene. The European Community Regulation No 2073/2005 defined a quantitative limit for 100 cfu.g-1 which was is applicable for certain types of food during their shelf life. However an adequate counting method for low contaminating levels is still lacking. The EN ISO 11290-2 European and International Standard method for enumeration of Listeria monocytogenes is characterized by a limit of enumeration of 10-100 cfu.g-1, and a poor accuracy. Recently a new method has been developed to enumerate L. monocytogenes at low contamination levels in several food matrices using a membrane filtration method (Baudouin et al., 2009). This method uses a combined enzyme/surfactant treatment to solubilize food components which increases handling period. The aim of the present work was to develop a new method to enumerate L. monocytogenes and Salmonella spp. at low concentration on meat products. This method is based on membrane filtration method without using treatment to solubilize food components. White pudding has been used as “experimental matrix” and artificially contaminated on surface with 4-12 cfu.g-1 of L. monocytogenes or Salmonella spp. For enumeration, 25 g of samples were placed into a plastic bag and homogenised in water peptone buffer (dilution 1/10) for 30 sec using a Pulsifier. Food suspension was filtered on a single use membrane of 47 mm diameter and 0.45 µm pore size. The filters were laid on specific plated media. To compare with a control, 25 g of samples were placed in classic Stomacher bags with a mesh screen liner (280 µm pore size) and homogenised using a Mix 2 Stomacher apparatus with the same dilution as above. A volume of 1 ml of filtered food suspension was laid on specific plated media according to EN ISO 11290-2 standard. Enumeration results obtained with the two methods are statically similar but the new one is easier, cheaper to carry out, and more sensitive. Moreover this methodology is faster than methods previously described and non destructive: food components solubilisation step is no more necessary. Preliminary tests were carried out using minced meat and, due to meat texture, problems with clog membrane occurred, therefore this method should be improved. This new method is useful for simulating the contamination level commonly found in food industries. [less ▲]

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See detailLa chondroitine sulfate stimule la production des facteurs anti angiogéniques TSP-1 et VEGI par les synoviocytes arthrosiques : un nouveau mécanisme d’action pour cette molécule.
Mathy, Marianne ULg; Lambert, Cécile ULg; Dubuc, Jean Emile et al

Poster (2010, September)

: La chondroïtine sulfate (CS), la glucosamine sulfate (GS), la glucosamine HCl (GH) et l’acide hyaluronique (AH) sont des anti-arthrosiques symptomatiques d’action lente (AASAL) dont l’utilisation est ... [more ▼]

: La chondroïtine sulfate (CS), la glucosamine sulfate (GS), la glucosamine HCl (GH) et l’acide hyaluronique (AH) sont des anti-arthrosiques symptomatiques d’action lente (AASAL) dont l’utilisation est recommandée dans le traitement de l’arthrose. Les mécanismes d’action de ces molécules demeurent mal compris. Notre étude a comme objectif d’étudier les effets de ces molécules sur la production de médiateurs impliqués dans l’angiogenèse de la membrane synoviale (MS) arthrosique. [less ▲]

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See detailSynovium angiogenesis in osteoarthritis: a new therapy target for chondroitin sulfate
Mathy, Marianne ULg; Lambert, Cécile ULg; Dubuc, Jean Emile et al

Poster (2010, September)

Osteoarthritis (OA) is an important cause of pain and disability in the ageing population. Angiogenesis and inflammation are closely integrated process in OA and may contribute to its pathogenesis, as ... [more ▼]

Osteoarthritis (OA) is an important cause of pain and disability in the ageing population. Angiogenesis and inflammation are closely integrated process in OA and may contribute to its pathogenesis, as well as, affect disease progression and pain. Chondroitin sulfate (CS) is a symptomatic slow acting drug for OA and there is strong evidence suggesting that CS may also be a structure disease modifying osteoarthritis drug. The mechanisms underlying these effects remain poorly understood. This work aimed to demonstrate the relation between inflammation and angiogenesis of synovium and to study the effect of CS on synovium angiogenesis. [less ▲]

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See detailPrediction of genetic interactions in yeast using machine learning
Schrynemackers, Marie ULg

Poster (2010, September)

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See detailFaux souvenirs et traitement hémisphérique dans le vieillissement.
Gajewski, Celine; Dehon, Hedwige ULg; Peigneux, Philippe ULg et al

Poster (2010, September)

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See detailNear-Death Experiences : Real or imagined memories?
Thonnard, Marie ULg; Laureys, Steven ULg; Brédart, Serge ULg et al

Poster (2010, September)

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See detailCotoneaster horizontalis on calcareous grasslands in Belgium : from ornament to management
Frisson, Gwenn ULg; Piqueray, Julien ULg; Halford, Mathieu ULg et al

Poster (2010, September)

Cotoneaster horizontalis DECAISNE, a Rosaceae coming from Asia, was introduced in Belgium as an ornamental species. Different sides of the invasion were studied, from the distribution of the species and ... [more ▼]

Cotoneaster horizontalis DECAISNE, a Rosaceae coming from Asia, was introduced in Belgium as an ornamental species. Different sides of the invasion were studied, from the distribution of the species and the invasion status, the impacts, to management methods. To describe the invasion status, information was gathered about the occurrence and the distribution of the species in Belgium by compiling various databases. The naturalization status in calcareous grasslands, high-value habitats recognized as biodiversity hotspots, was assessed. Occurrence, population status, preferred habitats, invaded communities, growth rate and fruiting capacity were characterized. Once naturalization established, we investigated its impacts on calcareous grasslands communities, individual species and pollinators. C. horizontalis occurred in seven of the nine studied calcareous grasslands, with densities from 0.34 to 10 individuals/ha. In the most invaded sites, an ongoing colonization process was suggested by a high proportion of small individuals. Moreover, 3-year-old individuals already showed fruiting capacities. Germination rate reached about 30%. Significant impacts on habitat structure and vegetation were showed. Community structure and composition changes were demonstrated in invaded sites by decreasing species richness and diversity. As calcareous grasslands are priority Natura2000 habitats, measures must be taken to control the spread of C. horizontalis. For this purpose, we performed management tests to identify the most efficient techniques that would help the restoration of calcareous grasslands. Both mechanical and chemical management techniques were tested. Methods choice was driven by the environmental constraints of calcareous grasslands, which require highly selective techniques, and by species characteristics. Tested methods were cuttings, stump burning, spraying, cutting plus glyphosate application on stump and glyphosate application on stem basis. Efficiency, cost, feasibility and side-effects on ecosystems were estimated. Management tests are still ongoing but some results have already been drawn: spraying and single cutting are not efficient, the last one generating production of numerous sprouts. Finding the best management method will not be sufficient to stop the invasion. C. horizontalis is still present in 53% of the sale catalogs of horticulturists in Southern Belgium. As long as the species is used in the vicinity of calcareous grasslands, propagules are continuously provided, jeoparding management actions. [less ▲]

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See detailDevelopment of an immobilisation device for treatment of patients after breast conserving surgery for mammary carcinoma.
COUCKE, Philippe ULg; WONNER, Michel ULg; JANVARY, Zsolt Levente ULg et al

Poster (2010, September)

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging ... [more ▼]

To develop an immobilisation device for prone positioning for breast irradiation. The basic requirements for the device are: rapid and accurate patient (re)positioning, compatibility with modern imaging devices and easy to handle by the treating technologists. [less ▲]

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See detailCan we really get rid of Japanese knotweed clones? Two years of management tests in Belgium
Monty, Arnaud ULg; Delbart, Emmanuel ULg; Mahy, Grégory ULg et al

Poster (2010, September)

Japanese knotweed Fallopia japonica is an extremely abundant invasive plant in Belgium and surrounding countries. To date, no eradication method is available for managers facing the invasion of this ... [more ▼]

Japanese knotweed Fallopia japonica is an extremely abundant invasive plant in Belgium and surrounding countries. To date, no eradication method is available for managers facing the invasion of this rhizomatous plant. We tested different chemical herbicides and two application methods (spraying and stem injection), as well as mechanical treatments, on Fallopia clones throughout southern Belgium. The tested methods were selected to be potentially usable by Belgian managers, e.g. using legally accepted rates for herbicides. Stem volume, height and density reduction was assessed after one or two years depending on the treatment. No tested method allowed a complete eradication of the clone. However, stem injection with glyphosate-based herbicide caused the highest damage. The year following injection, no sprouts were observed. Two following year, however, stunted shoots sprouted. Among mechanical control measures, repeated cuts combined with native trees cuttings plantations most appreciably reduced knotweed development. The most efficient methods we tested appear as tools for curbing knotweed invasion but are not likely to be used to eradicate the species. As such they should be included in a more integrated control strategy, together with prevention and public awareness campaigns. [less ▲]

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See detailEcology and evolution of invasive plants: what to study next?
Monty, Arnaud ULg; Brown, Cynthia; Tepolt, Carolyn et al

Poster (2010, September)

In recent decades, a growing number of studies have addressed connections between ecological and evolutionary concepts in biologic invasions. These connections may be crucial for understanding the ... [more ▼]

In recent decades, a growing number of studies have addressed connections between ecological and evolutionary concepts in biologic invasions. These connections may be crucial for understanding the processes underlying invaders’ success. However, the extent to which scientists have worked on the integration of the ecology and evolution of invasive plants is poorly documented, as few attempts have been made to evaluate these efforts in invasion biology research. Such analysis can facilitate recognize well-documented relationships and identify gaps in our knowledge. In this study, we used a network-based method for visualizing the connections between major aspects of ecology and evolution in the primary research literature. Using the family Poaceae as an example, we show that ecological concepts were more studied and better interconnected than were evolutionary concepts. Several possible connections were not documented at all, representing knowledge gaps between ecology and evolution of invaders. Among knowledge gaps, the concepts of plasticity, gene flow, epigenetics and human influence were particularly under-connected. We discuss five possible research avenues to better understand the relationships between ecology and evolution in the success of Poaceae, and of alien plants in general. [less ▲]

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See detailWhich evolutionary mechanisms allow Senecio inaequidens DC. to face a changing climate? A synthesis
Monty, Arnaud ULg; Mahy, Grégory ULg

Poster (2010, September)

Plant populations are expected to face gradual climatic variation in the next decades. Understanding and quantifying evolutionary and non-evolutionary mechanisms allowing populations response to climate ... [more ▼]

Plant populations are expected to face gradual climatic variation in the next decades. Understanding and quantifying evolutionary and non-evolutionary mechanisms allowing populations response to climate is therefore crucial to anticipate the actual consequences of climate change on plants. However, studying the direct effects of climate change in wild populations is of little anticipating interest… there invasive plants can help! We studied the sources of phenotypic variation in populations that gradually invaded different climatic zones over the last century, and assessed the role of local adaptation, non-adaptive genetic differentiation, phenotypic plasticity and environmental maternal effects in plant population response to climate. [less ▲]

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See detailThe influence of climate on hillslope sediment supply : Insights from numerical modeling
Bovy, Benoît ULg; Demoulin, Alain ULg; Braun, Jean

Poster (2010, September)

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See detailExperience feed back committee (EFBC) in radiotherapy
DELGAUDINE, Marie ULg; JANSEN, Nicolas ULg; COUCKE, Philippe ULg

Poster (2010, September)

Radiotherapy is a powerful continuously evolving effective treatment tool. Our aim is to offer the best treatments and assure security for patients and personnel. A proactive quality approach copied from ... [more ▼]

Radiotherapy is a powerful continuously evolving effective treatment tool. Our aim is to offer the best treatments and assure security for patients and personnel. A proactive quality approach copied from the one implemented in the air transport industry has been established in our department. An Experience feed back committee (EFBC) has been set up to identify, record and analyze systematically all reported precursor events. Our final objective is to test and strengthen the security of the organization and the quality of care for patients. [less ▲]

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See detailContribution to fight against counterfeit medecines applying several analytical tools
Marini Djang'Eing'A, Roland ULg; Mbinze Kindenge, Jérémie; Montes, M. L. A. et al

Poster (2010, September)

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See detailIN VIVO PET IMAGING OF THE EFFECT OF THE NMDA ANTAGONIST MEMANTINE ON GLUCOSE METABOLISM IN THE RODENT BRAIN
Warnock, Geoffrey ULg; Dedeurwaerdere, Stefanie; Bahri, Mohamed Ali ULg et al

Poster (2010, September)

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See detailScience with the Carlina hypertelescope
Le Coroller, H.; Dejonghe, J.; Regal, X. et al

Poster (2010, September)

Studies are currently underway to propose a generation of post-VLTI interferometers (Carlina, OHANA, Keops, etc.). Such interferometers will open new fields of research in astrophysics by imaging the ... [more ▼]

Studies are currently underway to propose a generation of post-VLTI interferometers (Carlina, OHANA, Keops, etc.). Such interferometers will open new fields of research in astrophysics by imaging the surfaces of supergiant stars, gravitational microlensing, AGN, Hot Jupiters, etc. To achieve these goals, they will have to respond to several criteria: to provide very high angular resolution (baselines > 100 m), to be equipped with a large number of mirrors (rich UV coverage), and to be able to accommodate high tech instrumentation such as an Adaptive Optics system and a coronagraph. We describe the optical Carlina architecture and show that it fulfills all these criteria. We give new results obtained with the prototype of Carlina currently built at Observatoire de Haute-Provence. Considering its expected specifications, Carlina will operate in complementarity with ELTs and very long baseline interferometers. [less ▲]

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See detailOptimisation and validation of a fast HPLC method for the quantitation of sulindac and its impurities
Krier, Fabrice ULg; Brion, Michaël; Debrus, Benjamin ULg et al

Poster (2010, September)

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See detailImpact of ageing and food additives on the physico-chemical properties of partially hydrolyzed and pregelatinized rice flour
Mertens, Cécile ULg; Blecker, Christophe ULg

Poster (2010, August 25)

Conservation of the quality of a food product is an essential preoccupation for industrials. Indeed, food acceptability by consummers depends of this quality. However, the latter decreases with time of ... [more ▼]

Conservation of the quality of a food product is an essential preoccupation for industrials. Indeed, food acceptability by consummers depends of this quality. However, the latter decreases with time of storage, due to complex alteration reactions. This study presents a first approach of alteration reactions in a particular range of products: drum-dried pregelatinized cereal products. While this type of product is widely used as thickeners or in infant foods, no study has ever been made on the subject. The cereal studied here is one of the most consumed by mankind: rice. We also investigated the impact of food additives (E170 & E340ii) on the ageing of such a product, because they are often used empirically in industry. [less ▲]

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See detailLiposomes loaded with diglyceride esters of methotrexate and mephalan studies on stability and hemocompatibility
Kuznetsova, N; Kandyba, A.K; Bovin, N et al

Poster (2010, August 24)

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See detailInfluence of steam explosion and thermal degradation of cellulose
Jacquet, Nicolas ULg; Quiévy, Nicolas; Vanderghem, Caroline ULg et al

Poster (2010, August 20)

The aim of the present work is to compare the effect of different steam explosion pretreatments on the thermal degradation of a bleached cellulose where components like hemicelluloses and lignin have ... [more ▼]

The aim of the present work is to compare the effect of different steam explosion pretreatments on the thermal degradation of a bleached cellulose where components like hemicelluloses and lignin have already been removed by acid and alkaline treatments. The results of this study show that thermal degradation of cellulose fibres, studied by TGA, is still limited for a temperature process below 240 °C. However, derivative TGA show that thermal stability of cellulose obtained by these conditions decreases with the increase of temperature. For temperatures above 250°C, char level is higher at the end of the pyrolysis. According to the literature, the increase of the char level is correlated to an increase of the degradation product1. Determination of the degradation products in the liquor obtained after the pretreatment show an important increase of furfural and 5-hydroxymethylfurfural concentration with the temperature in agreement with the increase of the char level. These results confirm the important degradation of the cellulose fibres. [less ▲]

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See detailCan we predict troubles during horse clinical examination by a simple test?
Peeters, Marie ULg; Godfroid, Sandra; Sulon, Joseph et al

Poster (2010, August 03)

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See detailEstimation of Genetic Parameters for Test Day Milk Yields of Moroccan Holstein Cows Using Random Regression Test Day Model
Tijani, Aziz; Rgayai, Brahim; Hammami, Hedi ULg et al

Poster (2010, August 02)

Detailed reference viewed: 46 (8 ULg)
See detailThe impact of diet in shaping microbiota revealed by a comparative study in children from Europe and Rural Africa
De Filippo, C.; Di paola, M.; Ramazzotti, M. et al

Poster (2010, August)

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See detailInvolvement of odorant cues in the process of superparasitism avoidance
Verheggen, François ULg; Vandermoten, Sophie ULg; Frere, Isabelle et al

Poster (2010, August)

The ability to avoid superparasitism provides a selective advantage to parasitoid females, allowing them to avoid depositing eggs in lower quality host. We observed in a Y-olfactometer that generalist ... [more ▼]

The ability to avoid superparasitism provides a selective advantage to parasitoid females, allowing them to avoid depositing eggs in lower quality host. We observed in a Y-olfactometer that generalist aphid parasitoids, Aphidius ervi and Aphidius rhopalosiphi (Hymenoptera, Braconidae), were more attracted toward non-parasitized than parasitized Sitobion avenae (Hemiptera, Aphididae) colonies. We collected the odors released from healthy aphids and aphids parasitized for 2 and 6 days using an electronic nose. Sitobion avenae alarm pheromone, (E)-ß-farnesene (EßF), was the only chemical identified, and was found in lower quantities in parasitized aphids. Both parasitoid species provided pronounced electrical depolarizations to EßF in electroantennography (EAG), and both were attracted to the latter compound in the Y-olfactometer. Parasitoid attraction was known to be guided by a variety of odorant cues released by plants and hosts, and our results support the hypothesis that the aphid alarm pheromone acts as a kairomone for A. ervi and A. rhopalosiphi. [less ▲]

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See detailICT Challenges in Belgian Businesses: an overview
Lisein, Olivier ULg

Poster (2010, August)

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See detailDo aphids and their predators use the same OBP to transport a same odour?
Vandermoten, Sophie ULg; Fan, Jia; Liu, Yong et al

Poster (2010, August)

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See detailWear study on polymers and bio-materials by ultra thin layer activation
Delhalle, René ULg; Marchal, André ULg; Lucas, S. et al

Poster (2010, August)

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See detailInvolvement of odorant cues in the process of superparasitism avoidance
Vandermoten, Sophie ULg; Frere, Isabelle; Hance, Thierry et al

Poster (2010, August)

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See detailTransport and interaction blockade of cold bosonic atoms in a triple-well potential
Schlagheck, Peter ULg; Malet, Francesc; Cremon, Jonas C. et al

Poster (2010, August)

We investigate the transport properties of cold bosonic atoms in a triple-well potential that consists of two large outer wells, which act as microscopic source and drain reservoirs, and a small inner ... [more ▼]

We investigate the transport properties of cold bosonic atoms in a triple-well potential that consists of two large outer wells, which act as microscopic source and drain reservoirs, and a small inner well, which represents a quantum-dot-like scattering region. Such configurations can be realized by optical triple-well lattices generalizing the setup realized in the interaction blockade experiment by Cheinet et al. [Phys. Rev. Lett. 101, 090404 (2008)]. Bias and gate "voltages" are introduced in order, respectively, to tilt the triple-well configuration and to shift the energetic level of the inner well with respect to the outer ones. By means of exact diagonalization considering a total number of 6 atoms in the triple-well potential, we find diamond-like structures for the occurrence of single-atom transport in the parameter space spanned by the bias and gate voltages, in close analogy with the Coulomb blockade in electronic quantum dots. We demonstrate how one can infer the interaction energy in the central well from the distance between the diamonds, and discuss the possibility of realizing single-atom pumping across the quantum dot. [less ▲]

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See detailFemale fertility expression in Walloon dairy cattle
Vanderick, Sylvie ULg; Bastin, Catherine ULg; Gengler, Nicolas ULg

Poster (2010, August)

A useful method to express female fertility of the Walloon dairy cattle using direct and indirect female fertility information was considered. Data were based on results of the genetic evaluation of ... [more ▼]

A useful method to express female fertility of the Walloon dairy cattle using direct and indirect female fertility information was considered. Data were based on results of the genetic evaluation of pregnancy rate for 606,328 Holstein cows and on INTERBULL proofs of female fertility for 88,496 bulls. A direct female fertility index (DFF), using INTERBULL proofs, was developed from results of a principal component analysis carried out on 6 published foreign female fertility indexes. An indirect female fertility index (IFF) was also developed from ten other traits evaluated in Walloon Region. Theory of selection index was used to combine DFF and IFF in a global index called combined female fertility index (CFF). This index allowed young bulls to have better reliabilities with an average increase of 12% leading to extra 4,019 of publishable bulls for female fertility. [less ▲]

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See detailHammett Acidity Scale in Ionic Liquids : An Indication of Their Weak Dissociating Character
Robert, Thierry ULg; Magna, Lionel; Olivier-Bourbigou, Hélène et al

Poster (2010, August)

Ionic liquids are salts with the particularity to exhibit melting points near room temperature (below 100°C, by convention) with no vapour tension. For this last reason, ionic liquids are sometimes called ... [more ▼]

Ionic liquids are salts with the particularity to exhibit melting points near room temperature (below 100°C, by convention) with no vapour tension. For this last reason, ionic liquids are sometimes called “green solvents”. In addition, their exclusive materials and solvent properties has led to an amazing increase of interest from both academic and industrial community, confirmed by the explosion of the number of published papers in the last decade. The many combinations of organic and inorganic cations and anions allow an infinity of new ionic solvents then permitting the selection of the desired properties for a given application. Nevertheless, it is impossible to investigate all these combinations and the unusual complexity of these new solvents gives rise to many controversies. Consequently, the development of the general rules for understanding the chemistry in ionic liquids is crucial. A fundamental property of solvent is its solvating power, for instance towards the proton. Therefore, we are interested to investigate the acid-base properties in ionic liquids in order to ultimately find a correlation with the acidic catalysis activity. We then have proposed a colorimetric method to determine the acidity levels accessible in these new media: the Hammett acidity function H0. This spectroscopic method is based on the protonation equilibrium for a family of coloured indicator with pKa’s assumed as solvent independent (following the Hammett proposition). This presentation will summarize our Hammett acidity measurements in several ionic liquids. - At first, we will show that it is possible to evaluate the Hammett acidity function with two different coloured indicators, in the same ionic liquid. Since the Hammett acidity of a given mixture was found to depend on the choosen coloured indicator, this suggests the formation of ions associations in ionic liquids. As a result, the ionic liquids are clearly not as dissociating as initially thought and the Hammett acidity function is in fact an apparent function, underestimating the real acidity level. - The apparent acidity functions have then been compared for several ionic liquids to which an acid has been added ([BMIm][NTf2], [BMIm][BF4], [BMIm][OTf], [BHIm][NTf2], [BMIm][PF6], [HNEt3][NTf2]). The conclusions are as follows: 1) the accessible acidity level is not influenced by the nature of the cation; 2) on the contrary, the nature of anion is very critical and the solvating power towards the proton follows the order: OTf- > NTf2- > BF4- > PF6-. The more the proton is solvated, the less it is acidic. - Finally, the difference of acidity of two acids, HOTf and HNTf2 (both strong acids in water), has been investigated in [BMIm][BF4], [BMIm][NTf2] and [BMIm][OTf]. In [BMIm][OTf], these two acids show the same acidity (they behave as strong acids) due to the solvent levelling effect; on the other hand, in [BMIm][NTf2] and [BMIm][BF4] allowing higher acidity levels, HNTf2 is stronger than HOTf . The observed difference is also another indication of the lower proton solvation in [BMIm][BF4] or [BMIm][NTf2] versus that in [BMIm][OTf]. [less ▲]

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See detailFirst-Principles Study of Oxidation Mechanism for SiC-based Materials
Xu, Bin ULg; Kroll, Peter

Poster (2010, August)

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See detailFungal gene expression in a forest soil
Kellner, Harald ULg; Portetelle, Daniel ULg; Vandenbol, Micheline ULg

Poster (2010, August)

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See detailChilling of heavy carcasses from double muscled cattle: time-temperature evolution and predictive modelling of growth of Listeria monocytogenes and Clostridium perfringens
Delhalle, Laurent ULg; Collignon, Bertrand; Dehard, Sandrine ULg et al

Poster (2010, August)

The time/temperature combination during carcass chilling is of concern in order to avoid bacterial growth. The chilling speed is lower in carcasses with high muscular development such as large cattle from ... [more ▼]

The time/temperature combination during carcass chilling is of concern in order to avoid bacterial growth. The chilling speed is lower in carcasses with high muscular development such as large cattle from the Belgian Blue breed. Three slaughterhouses were selected for temperature and pH measurements during the chilling process at 6 different days on 4 half carcasses in order to obtain representative data from heavy carcasses with high muscular development. Predictive microbiology was used to evaluate the potential growth of Listeria monocytogenes and Clostridium perfringens on the surface and in the depth of the carcasses. The gamma concept was chosen as secondary model taking into account the effect of temperature, pH and water activity on the selected bacteria during the chilling process. The predicted growth potential of Listeria monocytogenes is influenced by the different environmental conditions of the selected slaughterhouses and could reach 1.4 log CFU/cm² after the chilling process. The potential growth of Clostridium perfringens is limited due to unfavourable conditions during the first hours and to low temperature later. It can be concluded that when the initial level of contaminating bacteria is not excessive the speed at which the carcass is currently chilled is sufficient to limit the growth of these two pathogens and to ensure the product quality [less ▲]

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See detailGrowth monitoring of Listeria monocytogenes and Salmonella spp. according to the packaging technique in pork minced meat
Adolphe, Y.; Delhalle, L.; Jasick, A. et al

Poster (2010, August)

A high level of protection of public health is one of the fundamental objectives of European food laws, as laid down in Regulation (EC) No 2073/2005. Listeria monocytogenes is a pathogen found within the ... [more ▼]

A high level of protection of public health is one of the fundamental objectives of European food laws, as laid down in Regulation (EC) No 2073/2005. Listeria monocytogenes is a pathogen found within the food-processing industries, mainly brought by human, and was responsible of 1,381 confirmed cases of foodborne listeriosis for 2008 in Europe. Salmonella was in 2008, the second most often reported zoonotic disease in humans, and 131,468 confirmed cases of human salmonellosis were reported in Europe. The aim of the present work was to develop and to validate a quicker and more sensitive genetic method for quantification of L. monocytogenes and Salmonella spp. in meat products by the quantitative real-time PCR. After preliminary tests for primers and probes choice, the genetic method was validated with classical microbial methods (ISO 11290-2:1998, ISO 6579) using challenge tests. A mixture of 3 strains was realized for each bacterial genus: 1 referee strain (L. monocytogenesT NTCT 11994 or S. TyphimuriumT ATCC 14028) and 2 lab isolates. Experiments were carried out on pork's irradiated and not irradiated minced meat. These meats were packaged either in expanded polystyrene trays wrapped with permeable stretch film or under modified atmosphere (70% O2/30% CO2) in sealed trays. The initial inoculum (102 cfu.g-1) was homogenized in minced meat before packaging in trays. They were incubated for 14 days, at +5, +8 and +10°C and at +10 and +12°C, for respectively L. monocytogenes and Salmonella spp. In the 2 tested meats, growth speeds as well as the final populations increased according to the temperature. In the not irradiated minced meat, growth speeds of the pathogenic flora as well as the associated final populations were lower than those observed in the irradiated matrices. As soon as the total flora reached its stable growth phase, growths of the various pathogenic stagnated. The growth of the original flora inhibited partially the growth of pathogens inoculated. Generally, growth of the total flora is partially inhibited by modified atmosphere packaging. The analytical methods of molecular biology allow faster and less heavy analyses, in terms of hand of work and execution, than the methods of classic microbiology. [less ▲]

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See detailImpacts of growth development and age at first calving on first lactation yield in Holstein dairy cows
Picron, Pascale ULg; Froidmont, Eric; Turlot, Amélie et al

Poster (2010, August)

In a context of economic constraints, reducing the herd replacement costs represents a way to improve the economic efficiency of the dairy sector. Studies reported that heifers calving between 23 and 24.5 ... [more ▼]

In a context of economic constraints, reducing the herd replacement costs represents a way to improve the economic efficiency of the dairy sector. Studies reported that heifers calving between 23 and 24.5 mo of age achieved the highest economic return. However, the heifer has to be well developed to guarantee milk performance, calving ease and low stillbirth rate. Monitoring growth, by Heart Girth (HG) measurements, constitute a convenient way to evaluate the feasibility to inseminate heifers early. The aim of this study was to analyse the relationship between HG, age at first calving and milk production at first lactation. HG measurements of 704 Walloon (Belgium) Prim Holstein heifers have been recorded, every 3 months, during 2 years (2006 – 2008). The impact of development, at key-ages (from 3 to 24 mo, every 3 mo), on age at first calving and first lactation yield, was determined using analysis of variance. Mean total milk production in February 2010 (n = 319) rose to 7686 ± 1388 l/365 days of lactation, for a mean age at first calving (n = 421) of 27.4 ± 3.5 mo. These preliminary results suggest a positive correlation between growth performances and first calving age. Heifers with a better conformation (HG > 2.5 % of the French reference) calved significantly earlier than smaller animals. For instance, a lack of conformation at 15 mo implied a delay at first calving of 4.1 mo of age. HG measurement should therefore constitute a preferential tool of management of heifers herd. First lactation yield was significantly higher (950 l/365 d) for heifers calving older (> 30 mo) comparatively to early calving animals (24 mo). These results do not correspond to those related to larger samples in Wallonia. No significant relation could state, at this time, a direct relation between growth development and first lactation yield. Lactation data are still collected to complete this study. [less ▲]

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See detailProcessing of linguisitic and musical dimensions contained in songs : effects of an expertise
Larrouy, Pauline ULg; Leybaert, Jacqueline; Kolinsky, Régine

Poster (2010, August)

Songs are universal in our lives and also offer an ecological material that links linguistic and musical information. It therefore provides a tool for observing the relationship between music and language ... [more ▼]

Songs are universal in our lives and also offer an ecological material that links linguistic and musical information. It therefore provides a tool for observing the relationship between music and language. To examine the specificity of language and music information processing in songs, we studied the influence of expertise (i.e. musicians or speech therapists) on the use of transitional probabilities (TPs) in learning an artificial language (AL) either spoken or sung. This type of implicit statistical learning is especially important in language development (Saffran, Aslin, & Newport, 1996). We used the paradigm designed by Schön, Boyer, Moreno, Besson, Peretz and Kolinsky (2008). These authors have demonstrated the benefit of structural and motivational proprieties of music contained in the song in the learning process of AL in individuals with no expertise. Compared to a spoken AL, learning was enhanced for a singing AL, especially when the TPs of musical and linguistic materials were congruent. Our results did not replicate those of this study in participants having no music expertise. However, an interaction occurred between the type of AL and expertise of the participants. Indeed, only music experts seemed to benefit from the congruence between the TPs of musical and linguistic materials in extracting TP-based linguistic units. On the opposite, experts in language seemed to be favoured by the incongruence between the TPs of linguistic and musical information. These results are discussed in relation to the notions of the specificity of processing of lyrics and tunes in songs. [less ▲]

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See detailComparison of MRI images obtained at 7T in a dog to macroscopic and histopathological examination
Van Thielen, B.; Visser, F.; Denolin, V. et al

Poster (2010, July 20)

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See detailProteomic evolution of s.cerevisiae during chronological aging
Blomme, Arnaud ULg; Mac Cord, Allan ULg; Sluse, Francis ULg et al

Poster (2010, July 19)

Opposite to the replicative aging, which refers to the exponential decline in the capacity of a single cell to divide, chronological aging of the yeast Saccharomyces cerevisiae refers to the time period a ... [more ▼]

Opposite to the replicative aging, which refers to the exponential decline in the capacity of a single cell to divide, chronological aging of the yeast Saccharomyces cerevisiae refers to the time period a yeast cell can survive in a non-dividing state. In 2006, Allen and co-workers reported that yeast cells evolve into two cell types during stationary phase: a high density population defined as quiescent cells (Q) and a low density population defined as non-quiescent (NQ) cells. These two populations mainly differ by their viability, measured as the ability to form colonies when platted on Petri dishes, and can be separated by differential centrifugation on density gradient. In this work, we used the quantitative proteomics technique of 2DDIGE (two Dimensional Differential In-Gel Electrophoresis) to compare the evolution of the yeast cellular soluble proteome during chronological aging. We also checked the impact of the carbon source on stationary-phase cell differentiation. As the ratio of Q/NQ cells is decreasing with time, we have selected three distinct periods: 0 day (32h after outset of yeast culture on glucose, 100% of Q cells), 7 days (50% of Q cells) and 14 days (100% of NQ cells) to realize 3 proteomics comparisons (fig 1). [less ▲]

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See detailEffects of dietary methylmercury on the Zebrafish (Danio rerio) liver proteome
Brésart, David ULg; Fourdrilis, Séverine; Mathy, Grégory ULg et al

Poster (2010, July 19)

Methylmercury (MeHg) is an aquatic pollutant. It is produced from HgS by the action of sulphate-reducing bacteria and is released in fresh waters. MeHg is bioaccumulated through the trophic chain and is ... [more ▼]

Methylmercury (MeHg) is an aquatic pollutant. It is produced from HgS by the action of sulphate-reducing bacteria and is released in fresh waters. MeHg is bioaccumulated through the trophic chain and is known to cause different health troubles (trembling, memory loss, anemia and kidney deficiency). Toxic exogenous substances, such as MeHg, are transformed by liver’s metabolic pathway, making this the starting point of vertebrate detoxication. Almost 50% of MeHg assimilated in hepatocytes is accumulated in mitochondria (Ware et al.,1975) and It has been suggested that it may uncouples OXPHOS (Mori et al., 2007). The aim of this study was to identify the proteomics modifications of the liver mitochondrial proteome in response to a chronic MeHg intoxication by using the 2D DIGE methodology (Figure 1). Fishes were fed with two different contaminated diets (6.5 and 13.5 µg of MeHgCl / g of dry food.). We have also performed functional assays in order to confirm the MeHg uncoupling effect on Salmo truita liver mitochondria. [less ▲]

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See detailRole of Keratinocytes GPR109A and COX-2 in Nicotinic Acid and Mono-methyl Fumarate Induced Flushing
Hanson, Julien ULg; Gille, Andreas; Zwykiel, Sabrina et al

Poster (2010, July 17)

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See detailEFFECTIVENESS OF VOICE THERAPY RELYING ON THE USE OF EUTONY IN THE TRAITEMENT OF DYSPHONIC PATIENTS.
Martinot, Gisèle; Caner, Jessie; Morsomme, Dominique ULg

Poster (2010, July 15)

Twenty six patients with dysphonia (different etiologies) were submitted to a voice therapy program involving the use of Eutony. In order to assess its efficacy, pre and post-treatment audio recordings ... [more ▼]

Twenty six patients with dysphonia (different etiologies) were submitted to a voice therapy program involving the use of Eutony. In order to assess its efficacy, pre and post-treatment audio recordings were analysed and submitted to perceptual rating using GRBAS-I scale and acoustical analysis of fundamental frequency, jitter and shimmer. Aerodynamic data (Maximum Phonation Time & Estimated Subglottic Pressure) were also included in the voice quality assessment protocol. In addition , a questionnaire was sent to all patients to collect self-evaluation of voice quality and therapy outcomes. The study revealed a significant improvement lasting in time for two acoustic parameters : Jitter and Shimmer and five perceptual parameters : Grade, Roughness, Breath, Asthenia and Strain. Aerodynamic data showed slight improvements but no significant changes. Self evaluation questionnaires also tend to strengthen positive effects of therapy. Thus, this study supports the use of Eutony in vocal therapy for dysphonia. Its effects were shown to be measurable with both acoustical analysis and perceptual rating of voice quality. [less ▲]

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See detailContribution of "click chemistry" to the macromolecular engineering of aliphatic polyesters
Riva, Raphaël ULg; Schmeits, Stephanie ULg; Croisier, Florence ULg et al

Poster (2010, July 13)

In this work, click chemistry was sucessfully applied to the chemical modification of aliphatic polyesters with the purpose to tailor their physical properties. The developped strategy was then applied to ... [more ▼]

In this work, click chemistry was sucessfully applied to the chemical modification of aliphatic polyesters with the purpose to tailor their physical properties. The developped strategy was then applied to the synthesis of materials, such as smart partially degradable hydrogels or antibacterial polyesters. Last, the synthesis of amphiphilic star-shaped copolyester was investigated. [less ▲]

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See detailFacing the problem of the bis(acetylacetonato)cobalt(II)-mediated radical polymerization of acrylates
Hurtgen, Marie ULg; Debuigne, Antoine ULg; Jérôme, Christine ULg et al

Poster (2010, July 13)

Cobalt-Mediated Radical Polymerization (CMRP) is an efficient tool for polymerizing acrylates and vinyl esters. However, a challenge in CMRP remains in controlling the polymerization of both types of ... [more ▼]

Cobalt-Mediated Radical Polymerization (CMRP) is an efficient tool for polymerizing acrylates and vinyl esters. However, a challenge in CMRP remains in controlling the polymerization of both types of monomers with the same cobalt complex. Here, we show how Co(acac)2, efficient for unconjugated vinyl monomers, can also cope with acrylates. [less ▲]

Detailed reference viewed: 71 (4 ULg)
See detailStudy of oxylipins pathways by a transcriptomic analysis of two variety of
Ghars, Mohamed Ali ULg; Muhovski, Y.; Ghanem, M. et al

Poster (2010, July 11)

Detailed reference viewed: 31 (5 ULg)
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See detailFunctionalization of carbon xerogels for the preparation of Pd/C catalysts by grafting of Pd complex
Diverchy, C.; Hermans, S.; Job, Nathalie ULg et al

Poster (2010, July 11)

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See detailImmobilization of homogenous catalysts in nanostructured carbon materials
Gheorghiu, C. C.; Pérez-Cadenas, M.; Roman-Martinez, M. C. et al

Poster (2010, July 11)

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See detailIs there a generalized procedural deficit in children with Specific Language Impairment?
Gabriel, Audrey ULg; Maillart, Christelle ULg; Guillaume, Mélody et al

Poster (2010, July 08)

Previous studies (Lum et al., 2009; Tomblin et al., 2007; Ullman & Pierpont, 2005) have suggested that difficulties in the procedural learning system could contribute, in part, to the language ... [more ▼]

Previous studies (Lum et al., 2009; Tomblin et al., 2007; Ullman & Pierpont, 2005) have suggested that difficulties in the procedural learning system could contribute, in part, to the language difficulties observed in children with SLI. However, we have recently shown, with an adapted serial reaction time (SRT) task, that children with SLI are able to learn implicitly non-linguistic regularities (Gabriel et al., submitted). In this research, we wanted to determine whether children with SLI are able to learn an 8-elements probabilistic sequence into which irregularities are inserted. Assessing probabilistic sequence learning in children with SLI should help us to better circumscribe the language difficulties of these children [less ▲]

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See detailTiO2 thin films (1 µm) with ordered porosity used as high performance photoelectrode in DSSC
Dewalque, Jennifer ULg; Cloots, Rudi ULg; Dubreuil, Olivier et al

Poster (2010, July 06)

Mesoporous anatase thin films are very promising materials to act as electrode in dye-sensitized solar cells. Randomly oriented nanocrystalline TiO2 particles are usually used to prepare photoelectrodes ... [more ▼]

Mesoporous anatase thin films are very promising materials to act as electrode in dye-sensitized solar cells. Randomly oriented nanocrystalline TiO2 particles are usually used to prepare photoelectrodes with a thickness of 10-15 µm. Templated-assisted dip-coating techniques are used to obtain thin films with ordered porosity. The use of different templates influences the film porosity in terms of long-range order, percentage of porosity, pore size and pores connectivity. The photovoltaic performances of the samples have been evaluated. Such mesostructured films are compared in terms of photovoltaic performance with TiO2 nanoparticles films, generally used in DSSC. [less ▲]

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See detailDiversité des pucerons en parcelles de pomme de terre et impact sur la dissémination des viroses
Yattara, Almouner Ag Alhamis ULg; Véronique Genin, Lionel Hartert; Francis, Frédéric ULg

Poster (2010, July 05)

Protect crops against aphids is even less easy than the high proliferation potential and possible infestation by winged individuals from distant homes, require on the part of farmers regularly monitored ... [more ▼]

Protect crops against aphids is even less easy than the high proliferation potential and possible infestation by winged individuals from distant homes, require on the part of farmers regularly monitored. In addition, in the case of aphid vectors of virus diseases, it is necessary to detect the first attacks to limit the spread of the disease within the plot. In 2009, the abundance and diversity of aphids was studied using the yellow bins installed in a network of nine plots of potatoes throughout Wallonia. The evaluation of the diversity and abundance of aphid species were evaluated weekly by collecting the insects captured. At the same time catch and observations on the plots, a sample of thirty leaves of potato is taken from each of the nine plots and analyzed in the laboratory to detect any viral infections, mainly PVY and PLRV. During the growing season, six species of aphid were particularly abundant: Metopolophium dirhodum, Sitobion avenae, Aphis fabae, Macrosiphum euphorbiae, Rhopalosiphum padi and Acyrthosiphon pisum. The ELISA performed on leaf samples taken from different plots of potato revealed the presence of PVY and PLRV in six of nine plots sampled. We observed a greater diversity of aphid species in almost all plots investigated. [less ▲]

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See detailRetrospective evaluation of 9 horses with surgical treatment of keratoma
Salciccia, Alexandra ULg; Bouhmala, Nabil; Serteyn, Didier ULg et al

Poster (2010, July 02)

Detailed reference viewed: 79 (5 ULg)
See detailClotridial hydrogenases and the biohydrogen production
Calusinska, Magdalena ULg; Hamilton, Christopher ULg; Masset, Julien ULg et al

Poster (2010, July 01)

Among the large variety of microorganisms capable of fermentative hydrogen production, strict anaerobes such as Clostridium spp. are one of the most widely studied. They produce hydrogen by butyric and ... [more ▼]

Among the large variety of microorganisms capable of fermentative hydrogen production, strict anaerobes such as Clostridium spp. are one of the most widely studied. They produce hydrogen by butyric and mixed-acid fermentations at optimal pH values ranging from 4.5 to 5.5. While fermentative conditions such as substrate type, pH, hydraulic and solid retention time, H2 partial pressure and the concentration of acids produced have been extensively studied and optimized, relatively little is known about the different forms of hydrogenases present in clostridia. Building on previous reports [1, 2] and by analyzing sequenced genomes, we found that [FeFe] hydrogenases are not a homogenous group of enzymes, but exist in multiple forms with different modular structures and are especially abundant in Clostridum spp. [3]. However, among the numerous studies performed on fermentative hydrogen production by Clostridium sp., only a few are specifically concerned with hydrogenases. Even there the authors focus on one type of [FeFe] hydrogenase, (CpI-like) without considering the existence of multiple forms of this enzyme within one species. Therefore, we focused our research on the better characterization of different forms of hydrogenases present in the genus Clostridium. Using newly designed degenerate primers, specific for clostridial hydrogenases, we amplified different hydrogenases from our species of interest. Further, by designing specific qPCR assays we have quantitatively targeted different hydrogenases. By analyzing differential gene expression, according to applied growth conditions, we believe to optimize the hydrogen production process in order to achieve better production rates. To conclude, we think that a a precise knowledge of hydrogen metabolism and hydrogenases is essential to optimization of the biohydrogen production process and should therefore be a goal for future research. [less ▲]

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See detailA phylogenomic falsification of the chromalveolate hypothesis
Baurain, Denis ULg; Brinkmann, Henner; Petersen, Jorn et al

Poster (2010, July)

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See detailThe essential role of the plastidial glyceraldehyde- 3-phosphate dehydrogenase in viable pollen development in Arabidopsis
Muñoz-Bertomeu, Jesús; Cascales - Miñana, Borja ULg; Anoman, Armand D. et al

Poster (2010, July)

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See detailActivated-carbon-xerogel/TiO2-P25 composite photocatalysts with high activity for the degradation of p-nitrophenol
Páez Martínez, Carlos ULg; Contreras, María; Olivera-Fuentes, Claudio et al

Poster (2010, July)

Detailed reference viewed: 89 (12 ULg)