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See detailLipase-catalyzed interesterification of butterfat with rapeseed oil: new approaches for the monitoring of the reaction.
Hanon, Emilien ULg; Aguedo, Mario ULg; Danthine, Sabine ULg et al

Poster (2007)

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of ... [more ▼]

Butterfat (BF) is one main source of diet fats. However, it has been less and less well perceived due to its poor spreadability when refrigerated and cholesterol and saturated fatty acids, promoters of coronary heart diseases. Thus, consumer’s demand for healthy palatable fat spreads with good development of modified butter-based spreads. One ordinary method used by manufacturers for such modifications is enzymatic interesterification of a lipase to restructure triacylglycerides (TAG), i.e. to induce the exchange of fatty acid residues amongst glycerol backbones. This leads to changes in TAG species and in physical properties of the fat, namely in solid fat content (SFC) and in melting profile. Rapeseed oil (RO) contains a large amount of oleic acid and has significant contents of linoleic and linolenic acids, i.e. a high global content of unsaturation-rich residues. Thus, EIE of BF with RO may bring nutritional improvements to the reaction product, when compared to BF alone. The EIE of BF and canola oil (a low-erucic acid RO) catalyzed by the immobilized sn-1,3 specific Rhizopus arrhizus lipase in solvent-free batch and micro-aqueous systems, was previously studied. The aim of the present study was first to assess the evolution of chemical, physical and thermal modifications occurring during solvent-free batch EIE of BF and RO, with the use of lipozyme TL IM. The evolution of TAG profiles, interesterification degree, dropping point, solid fat content and free fatty acids was monitored during the reaction, especially during the first hours. Differential scanning calorimetry was also applied to follow the formed product. Then the establishment of relations between the DP and differential scanning calorimetry data and the interesterification degree was emphasized. [less ▲]

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See detailComputed tomography anatomy of the equine metacarpo-phalangeal joint
Vanderperren; Ghaye, Benoit ULg; Hoegaerts, Michel et al

Poster (2007)

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See detailFeasibility of a urine-based DNA methylation assay for early detection of bladder cancer
Renard, Isabelle; Kelly, J.; Collette, Catherine et al

Poster (2007)

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See detailDevelopment of urine-based DNA methylation assay for prostate cancer screening
Vener, T. I.; Derecho, C.; Varde, S. et al

Poster (2007)

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate ... [more ▼]

Introduction: The best outcome for patients with prostate cancer (PCa) is seen for those treated at an early stage of the disease. A digital rectal examination (DRE) and the measurement of serum prostate specific antigen (PSA) levels are the current standards for PCa early detection. However, serum PSA testing lacks both sensitivity and specificity, and core biopsies frequently fail to identify small foci of PCa. The availability of non-invasive diagnostic molecular tests that could allow for a more precise identification of malignant prostate cells in asymptomatic men would be of great clinical value to improve PCa diagnosis. Study design: 114 men scheduled to undergo a prostate biopsy were enrolled in the study. The biopsies were triggered either by an abnormally high PSA value or by suspicious findings on DRE. Patients with other known or suspected urinary malignancy were excluded from the study. Morning, post-prostate massage and post-biopsy urine samples were collected from all individuals. The main goals of this study were a) to determine if prostate massage can improve the prostate DNA quantity compared to urine collected in the morning or after biopsy, and b) to evaluate the methylation status of a gene panel in urine samples from subjects with cancer found in prostate biopsy tissue cores versus subjects without cancer. Methods: Gene promoter methylation is associated with prostate cancer and has been successfully used for the molecular detection of neoplasia in urine. We have developed real-time methylation specific PCR assays to define the methylation status of several genes. Results: Median age of the patients was 65 years (range 48-85). PCa was found in 51% of the patients. Histological diagnosis of the biopsies was compared to methylation results in urine from 102 samples (89% success rate due low DNA yields for 12 samples). The comparison between different urine sampling techniques showed that prostate massage is needed. The best results were obtained in post massage urine samples with a combination of GSTP1, p14, p16, RARβ2 and RASSF1A resulting in a sensitivity of 74% and a specificity of 75%. Future: A multiplex assay using the Cepheid SmartCycler™ II platform is under development. Further studies are in progress to validate the assay across multiple centers. [less ▲]

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See detailUltrastructural organization of the reptilian nucleolus
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

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See detailUltrastructural organization of the nucleolus in reptiles
Lamaye, Françoise; Thiry, Marc ULg

Poster (2007)

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See detailSound production mechanism in the clownfish Amphiprion clarkii (Amphiprioninae, Pomacentridae)
Colleye, Orphal ULg; Herrel, Anthony; Mauguit, Quentin et al

Poster (2007)

Clownfishes live in social group within sea anemones. They are prolific “singers” that produce a wide variety of sounds, described as “pops” and “chirps”, involved in both reproductive and agonistic ... [more ▼]

Clownfishes live in social group within sea anemones. They are prolific “singers” that produce a wide variety of sounds, described as “pops” and “chirps”, involved in both reproductive and agonistic interactions. Although clownfish sounds were recorded since 1930, the mechanism of sound production has remained unresolved. The sounds used to describe the sonic mechanism were directed towards hetero- and conspecifics that approach their sea anemone host. Sound recordings were synchronized using a high speed video (500 fps) coupled or not with an X-ray system. These systems allowed to quantify the movements of external and internal bones during sound production. Sounds were typically accompanied by rapid (< 30 ms) head movements such as elevation of the skull, lowering of the hyoid bar and the anterior part of the branchial basket, retraction of the pectoral girdle, and finally closing of the mouth. Synchronization of sound pulses with X-ray images indicates that sound is produced when the hyoid apparatus is completely lowered and the mouth closed by a previously unknown mechanism. Dissections of freshly dead specimens reveal an unusual ligament responsible for the rapid mouth closing. This ligament joins the hyoid bar to the internal part of the mandible. Acting as a cord, it forces the mandible to turn around its articulation during the lowering of the anterior part of the branchial basket, forcing the mouth to close. Sounds result from the collision of the jaw teeth, transferring energy to the jaws that are presumably the sound radiator. [less ▲]

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See detailL'assiette du Belge en chiffres
Duquesne, Brigitte ULg

Poster (2007)

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See detailThe Grolier Codex: A PIXE & RBS study of the possible Maya document
Calvo Del Castillo, Helena ULg; Ruvalcaba Sil, Jose Luis; Calderón, Tomás et al

Poster (2007)

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See detailCharacterization of puff pastry margarines with and without TFA
Cavillot, V; Kervyn de Meerendré, M; Pierart, Céline ULg et al

Poster (2007)

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See detailEvaluation of the antitumor activity of 16K prolactin
Kinet, Virginie; Nguyen, Ngoc-Quynh-Nhu ULg; Cornet, Anne ULg et al

Poster (2007)

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See detailFibrous mucosa preservation with NobelGuide
Bolland, Fabrice; Van Heusden, Alain ULg

Poster (2007)

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See detailMG-63 Osteoblast culture on P culture on PLA– based copolymers for bone tissue engineering applications
Moniotte, Nicolas; Shim, Yong-Ho; Borget, Pascal et al

Poster (2007)

Physical injury or pathological changes such as removal of a tumor can result in large bone defects, preventing the recovery of its original function. Autogenous bone grafting, which is the most common ... [more ▼]

Physical injury or pathological changes such as removal of a tumor can result in large bone defects, preventing the recovery of its original function. Autogenous bone grafting, which is the most common technique for bone defect repairing, is associated with serious limitations, e.g. limited supply and donor site morbidity. Since a few years bone tissue engineering by degradable biomaterials has been shown as a very promising avenue for providing bone substitutes. Among these materials, bioresorbable synthetic polymers such as poly(ethylene oxide) (PEO), poly(vinyl alcohol) (PVA), poly(acrylic acid) (PAA) and poly(lactic acid) (PLA) are very appealing because their chemistry and properties are controllable and reproducible. Cellular activity and proliferation of osteosarcoma cell lines (MG-63) on films were determined by the tetrazolium salt MTT assay and by phase contrast/fluorescence microscope observations. The cytotoxicity of the materials was found to be low or negligible. Cells viability variations were observed on the surface of the films. Long-term cell culture and degradability of PLA-PEOpolymer film was investigated by optical microscopy (Giemsa staining) and environmental scanning electron microscopy (ESEM). Hydrolysis of the PLLA ester linkages led to slow film degradation. After 113 days, optical microscope observations revealed the presence of large cracks on the surface, and even breaks of small polymer fragments, while MG-63 proliferation was still very important, showing a tissue-like aspect, with extracellular matrix (ECM) deposition. These results show that PLA-PEO copolymers are very interesting bioresorbable materials for long-term bone tissue engineering applications. [less ▲]

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See detailThe lymphatic ring assay: a new in vitro model of lymphangiogenesis
Bruyère, F; Melen, L; Blacher, Silvia ULg et al

Poster (2007)

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See detailPotential of Shallow Lake Systems to Trace Environmental Changes Caused by Earthquakes
Avsar; Boes; Hubert-Ferrari et al

Poster (2007)

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See detailMajor and trace element geochemistry in a peat core from North Poland.Preliminary results
De Vleeschouwer; Fagel, Nathalie ULg; Cherbukin et al

Poster (2007)

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See detailAdenosine thiamine triphosphate, a new signal synthesized in response to specific forms of cellular stress in Escherichia coli
Gigliobianco, Tiziana ULg

Poster (2007)

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine ... [more ▼]

Thiamine, also known as vitamin B1, and its different phosphorylated derivates are found in all known organisms. The main form of the vitamin is the coenzyme thiamine diphosphate (ThDP). Thiamine triphosphate (ThTP) is also found in low amounts in all organisms studied from bacteria to mammals. In E. coli ThTP accumulation is linked to amino acid starvation and the presence of a suitable carbon source. Recently a new thiamine derivative, adenosine thiamine triphosphate (AThTP) or thiaminylated ATP was discovered in our laboratory. AThTP is accumulated in E. coli during carbon starvation. When the cells are grown under optimal conditions (rich medium containing amino acids) neither ThTP nor AThTP is present in significant amounts. The synthesis of both compounds is inhibited by amino acids. In E. coli, AThTP accumulates in response to two different conditions of metabolic stress: lack of energy substrates (or inhibition of their metabolization) and uncoupled pyruvate oxidation. [less ▲]

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See detailUnderstanding the irregularity of Seismic cycles: A Case study in Turkey- A Marie Curie Excellence Team Project
Hubert-Ferrari; Boes; Faser et al

Poster (2007)

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See detailLast two Millennia atmospheric lead and heavy metals inputs in a Belgian peat bog: regional to global Human impacts
De Vleeschouwer; Gerard; Goormaghtigh et al

Poster (2007)

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See detailCarbon dioxide dynamics in the tropical Ebrié lagoon (Ivory Coast)
Koné, Y. J. M.; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2007)

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See detailThe Mus musculus Mx1 gene confers strong protection against highly pathogenic influenzavirus A H5N1
Garigliany, Mutien-Marie ULg; Lambrecht, Bénédicte; Leroy, Michaël et al

Poster (2007)

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See detailComparison of different techniques for inoculation of Candidatus phytoplasma mali in biological indexing
Aldaghi, M.; Massart, Sébastien ULg; Roussel, S. et al

Poster (2007)

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See detailEuroPlaNet is celebrating a very special year
Chatzchristou, E. T.; Nazé, Yaël ULg

Poster (2007)

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See detailCaractérisation de la protéine 9p du virus de la varicelle et du zona (VZV)
Joris-Gerards, Aline; BONTEMS, Sébastien ULg; Di Valentin et al

Poster (2007)

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See detailCannibalism in Anchovy (Engraulis encrasicolus) in the North Aegean Sea (Greece)
Gennotte, Vincent ULg; Torre, Michele; Kallianiotis, Argyris

Poster (2007)

Egg and larval cannibalism was characterized in the European anchovy (Engraulis encrasicolus) during the spawning season in the North Aegean Sea. About 3 % of the sampled fish had consumed eggs and 7 ... [more ▼]

Egg and larval cannibalism was characterized in the European anchovy (Engraulis encrasicolus) during the spawning season in the North Aegean Sea. About 3 % of the sampled fish had consumed eggs and 7 % larvae. Egg consumption was observed during the night and larval consumption during the day. Cannibalism seems to be an opportunistic feeding strategy of anchovy, depending on prey availability and proximity of adults, larvae and eggs in relation with the diel vertical migrations of the fish. [less ▲]

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See detailDevelopment of an original intra-uterine implant allowed specific release kinetics of several drugs
Nizet, Dominique; Zalfen, Alina; Collard, Laurence ULg et al

Poster (2007)

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See detailFatty acids and stable isotopes in fish and harbour porpoises (Phocoena phocoena) from the North Sea: further insights in their trophic relationships
Drouguet, Olivier; Caut, Stéphane; Haelters, Jan et al

Poster (2007)

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises ... [more ▼]

Recent observations revealed an increased abundance of the harbour porpoise in the southern part of the North Sea. Concomitant to sightings of living individuals, the number of stranded porpoises displaying lesions linked to fishing nets has also increased this last decade. The increased abundance of porpoises together with the increase in numbers of bycaught animals raised the question of possible competition between porpoises and fishermen in the North Sea. Therefore, a detailed view on harbour porpoise trophic ecology in this region is crucial for their conservation. Stomachs of the washed ashore porpoises were often empty and provided limited information on their recent diet. Another way of looking into the diet of marine mammals is the analysis of blubber. Stable isotopes ratio (d13C and d15N, SI) and fatty acid composition (20 fatty acids, FA) were analyzed in muscle and blubber of 10 freshly stranded harbour porpoises and in 60 potential prey fish from 10 species collected in the North Sea. The SI ratio and FA composition of these fish enabled a clear classification in different trophic levels and different trophic niches. The FA composition was very similar between harbour porpoise blubber and demersal fish, underlining a preferential predation on that resource for the porpoises investigated. A bias might exist for the porpoises: most of the animals were juveniles, and washed ashore during spring. Porpoises might display other feeding habits in other life stages, other periods of the year. These preliminary results however indicate the enhanced accuracy of crossing these biomarker approaches. [less ▲]

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See detailPeculiar hydrophobic properties of the 67-78 fragment of α-synuclein are responsible for membrane destabilization and neurotoxicity
Crowet, Jean-Marc ULg; Lins, Laurence ULg; Dupiereux-Fettweis, Ingrid ULg et al

Poster (2006, December 18)

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ... [more ▼]

α-synuclein is a 140 residue protein linked to Parkinson’s disease. Intraneural inclusions called Lewy bodies and Lewy neurites are mainly composed of α-synuclein aggregated in amyloid fibrils. Few years ago, tilted peptides have been detected in two other amyloidogenic proteins : the amyloid β peptide involved in Alzheimer’s disease, and the PrP protein linked to Creuztfeldt-Jakob’s disease. Tilted peptides are short protein fragments that adopt an oblique orientation when inserted into biological membranes. Tilted peptides are able to destabilize membranes. In this study, we predicted by sequence analysis and molecular modelling that the 67-78 fragment of α-synuclein is a tilted peptide. Like most of them, the α-syn 67-78 peptide is able to induce lipid mixing and leakage of unilamellar liposomes. A mutant designed by molecular modelling to decrease the destabilizing properties of the peptide was shown to be significantly less fusogenic. The neuronal toxicity was studied using human neuroblastoma cells and we demonstrated that the α-syn 67-78 peptide induces neurotoxicity. In conclusion, we have identified a tilted peptide in α-synuclein which could be involved in the toxicity induced during amyloidogenesis of α-synuclein. [less ▲]

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See detailMolecular cloning and functional expression of a new aphid isoprenyl diphosphate synthase
Vandermoten, Sophie ULg; Beliveau, Catherine; Sen, Stephanie et al

Poster (2006, December 18)

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See detailSCREENING OF SLEEP APNEAS AND HYPOPNEAS THROUGH THE AUTOMATIC ANALYSIS OF MIDSAGITTAL JAW MOTION
Senny, Frédéric ULg; Destiné, Jacques ULg; POIRRIER, Robert ULg

Poster (2006, December 07)

This paper proposes a novel method for the scoring of sleep apneas and hypopneas (SAHs) based on the recording and the analysis of the midsagittal jaw movements. Continuous wavelet transform was used to ... [more ▼]

This paper proposes a novel method for the scoring of sleep apneas and hypopneas (SAHs) based on the recording and the analysis of the midsagittal jaw movements. Continuous wavelet transform was used to delineate events which were likely to contain the SAHs, while hidden markov models (HMMs) classified events. Considering 28 recordings from which awakenings were discarded, the method detected SAHs with a sensitivity and a specificity of 82.2% and 78.3% respectively. Obstructive, central and mixed respiratory events were distinguished fairly accurately. The jaw motion is hence a reliable marker of respiratory efforts and may suffice by itself to score SAHs. [less ▲]

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See detailImportancia de la elección de distintos oligonucleótidos para el diagnostico de BLV por PCR en productos de origen bovino
Trono, K.; Gutiérrez, G.; Rodriguez, Sabrina ULg et al

Poster (2006, December 05)

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See detailA comparative study of O2 measurements in experimental (Interice II) and natural (ISPOL, Western Weddell Sea, Antarctica) first-year sea ice
Tison, Jean-Louis; Mock, T.; Thomas, David et al

Poster (2006, December)

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See detailMapping of the bovine growth hormone secretagogue receptor (GHS-R) and polymorphism study in cattle
Colinet, Frédéric ULg; Eggen, André; Gengler, Nicolas ULg et al

Poster (2006, December)

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and ... [more ▼]

A third control pathway of the Growth Hormone (GH) secretion has come into picture since the development of synthetic compounds known as Growth Hormone Secretagogues (GHSs). The GHS Receptor (GHS-R) and its subtype are abundantly located in the hypothalamus-pituitary unit, but are also distributed in other central areas and peripheral tissues. The GHS-R belongs to the G-protein coupled receptor family with seven transmembrane domain architecture. In order to determine the GHS-R gene sequence, total mRNA was extracted from abomasum and two types of GHS-R cDNA were identified. These two types are transcript variants (1a and 1b) of the same GHS-R gene. The gene encompasses two exons and a single intron. Using a 3000 Rad hybrid panel, the GHS-R gene was mapped to Bos taurus autosome 1 (BTA 1). This localization on BTA 1 agrees totally with comparative data between cattle and human since BTA 1 corresponds to part of human chromosome 3 where human GHSR is also mapped. By two-point analysis, most significantly linked marker are BL26 and BMS4031 (both LOD score : 5,66). Some studies detected different QTLs near these markers like for growth rate, carcass yield, milk portein and milk yield. In the cattle industry, it is of economical importance to increase plasma GH secretion because it is associated with faster growth, less fat stores and improved milk production. Being of economical importance and the detected QTLs near the GHS-R gene, it would be interesting to study the polymorphism on the bovine GHS-R gene. Screening for polymorphisms in the two exons on ten Belgian Blue bulls, ten Holsteins bulls and ten Limousin bulls revealed a total of four single nucleotide polymorphisms (SNPs): three SNPs are in the first exon and one SNP in the second exon. In order to evaluate if GHS-R could be involved in genetic variation for growth rate, carcass yield, milk portein and milk yield, an association study between SNPs on GHS-R gene and these traits could be performed in a major cattle population. [less ▲]

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See detailInferring Groups of Correlated Failures
Lepropre, Jean; Leduc, Guy ULg

Poster (2006, December)

We compare and evaluate different methods to infer groups of correlated failures. These methods try to group failure events occurring nearly simultaneously in clusters. Indeed if several failures occur ... [more ▼]

We compare and evaluate different methods to infer groups of correlated failures. These methods try to group failure events occurring nearly simultaneously in clusters. Indeed if several failures occur nearly at the same moment in a network, it is possible that these failures have the same root cause. The input data of our algorithms are IP failure notifications that can be provided by several sources. We consider two sources: IS-IS Link State Packets (LSPs) and Syslog messages. Our first results on the Abilene and GÉANT networks show that the inference methods behave differently and that using IS-IS LSPs provides more accurate results than using Syslog messages. [less ▲]

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See detailMicro-macro mechanical modeling of bone-implant interface by means of the homogenization theory
Amor, Nadia; Van Cleynenbreugel, Tim; Geris, Liesbet ULg et al

Poster (2006, December)

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See detailOn the understanding of the physical changes in inulin powder as a function of water activity.
Ronkart, Sébastien; Paquot, Michel ULg; Goffin, Dorothée ULg et al

Poster (2006, December)

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See detailEnCOrE : Encyclopédie de Chimie Organique Electronique
Colaux, Catherine ULg; krief, Alain

Poster (2006, December)

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See detailBuilding EnCOrE : Encyclopédie de Chimie Organique Electronique
Colaux, Catherine ULg; Seleck, Caroline; Henry, Julie et al

Poster (2006, December)

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See detailInfluence de la variété de blé tendre sur les caractéristiques physico-chimiques et les propriétés techno-fonctionnelles de l'amidon
Massaux, Carine; Lenartz, Jonathan; Bodson, Bernard ULg et al

Poster (2006, November 20)

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See detailNew developments in benzopyran derivatives as pancreatic β-cell KATP channel openers
Florence, X.; De Tullio, Pascal ULg; Lebrun, P. et al

Poster (2006, November 18)

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See detailPharmacological evaluation of TP receptor antagonists by differential activity on alpha and beta isoforms
Hanson, Julien ULg; Dogne, J. M.; Ghiotto, J. et al

Poster (2006, November 18)

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See detailUso del Inmunoblot como método diagnóstico confirmatorio de la Anemia Infecciosa Equina
Álvarez, I.; Vissani, A.; Gutiérrez, G. et al

Poster (2006, November 12)

USO DEL INMUNOBLOT COMO MÉTODO DIAGNÓSTICO CONFIRMATORIO DE LA ANEMIA INFECCIOSA EQUINA Immunoblotting as confirmatory diagnostic assay for Equine Infectious Anemia Alvarez Irene 1 ialvarez@cicv.inta.gov ... [more ▼]

USO DEL INMUNOBLOT COMO MÉTODO DIAGNÓSTICO CONFIRMATORIO DE LA ANEMIA INFECCIOSA EQUINA Immunoblotting as confirmatory diagnostic assay for Equine Infectious Anemia Alvarez Irene 1 ialvarez@cicv.inta.gov.ar , Vissani Aldana1, Gutierrez Gerónimo1, Rodríguez Sabrina 1, Barrandeguy María 1 y Trono Karina 1. 1Instituto de Virología – CICVyA – INTA . Las Cabañas y Los Reseros s/n. 1712. Castelar Argentina. INTRODUCCION Y OBJETIVO. La prueba de diagnóstico “Gold Standard” para la Anemia Infecciosa Equina (AIE) continúa siendo la Inmunodifusión en gel de agar (IDGA), y aunque existen ensayos de ELISA comerciales, la mayoría de los diagnósticos se realizan en la actualidad por IDGA. En ciertas ocasiones no se puede declarar el estado serológico del animal debido a la presencia de reacciones inespecíficas que no permiten obtener un resultado concluyente por IDGA o se ha obtenido una respuesta diferente cuando se analizó la misma muestra de suero por IDGA y ELISA. Con el objetivo de contar con un método de diagnóstico que permita confirmar y calificar correctamente el estado serológico referido a la AIE, se evaluó en este trabajo un ensayo de Inmunoblot diseñado en nuestro laboratorio, que utiliza como antígeno proteína viral p26 recombinante (Inmunoblot-rp26). MATERIAL Y METODO. Un panel de sueros equinos evaluado previamente por IDGA (DyaSystems EIA-AGID test kit, Idexx Laboratorios) fue analizado por Inmunoblot-rp26 utilizando proteína recombinante p26 purificada fijada a membrana de PVDF. El panel incluyó: 74 sueros de campo positivos, 95 sueros de campo negativos, 63 sueros de campo inespecíficos, 26 sueros de equinos con anticuerpos frente a otras enfermedades infecciosas y 8 con alto grado de hemólisis. Además, se evaluaron para obtener un estándar de comparación, 5 paneles de proficiencia de 12 sueros cada uno otorgados por SENASA, 1 panel de proficiencia de 20 sueros otorgado por NVSL-USA y el suero positivo débil de referencia de la OIE para Anemia Infecciosa Equina. RESULTADOS. Los resultados obtenidos con el Inmunoblot-rp26 fueron comparados con los resultados del IDGA. Los resultados discordantes fueron analizados por Inmunoblot utilizando como antígeno la proteína de fusión en la región amino-terminal de p26 (inmunoblot-pBad). Todos los sueros positivos de campo fueron detectados como positivos por Inmunoblot-p26. De un total de 95 sueros negativos por IDGA, 6 fueron declarados como positivos por Inmunoblot-p26 y su estado confirmado por Inmunoblot-pBAD. Hubo concordancia total de resultados entre Inmunoblot-rp26 e IDGA con los paneles de proficiencia provenientes de SENASA y del NVSL. El suero de referencia de la OIE reaccionó con resultado claramente positivo, a diferencia de lo que sucede con IDGA, donde reacciona como débil positivo. Ninguno de los sueros con anticuerpos frente a otras enfermedades infecciosas, como tampoco los sueros bemolizados, presentaron reacción positiva por Inmunoblot-p26. Se obtuvo un claro resultado diagnóstico al analizar los sueros declarados “inespecíficos” por IDGA, entre los cuales se pudieron observar resultados negativos y positivos. CONCLUSION. El Inmunoblot con proteína p26 recombinante se comportó como una herramienta valiosa para el diagnostico confirmatorio de la anemia infecciosa equina. [less ▲]

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See detailEffects of vagal nerve stimulation in the rat orofacial formalin model of pain
Multon, Sylvie ULg; Scholsem, Martin; LEGRAIN, Caroline ULg et al

Poster (2006, November)

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See detailL'affirmation de soi par la pratique de l'acteur : évaluation d'un groupe thérapeutique
Delvaux, Muriel ULg; Roodthooft, Dominique; Sarto, Danielle et al

Poster (2006, November)

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de ... [more ▼]

Un groupe thérapeutique d’affirmation de soi a eu lieu au printemps 2006 au sein de l’Unité de Thérapies Comportementales et Cognitives de l’ULg. L’originalité du programme tient à l’association de techniques théâtrales à un programme de thérapies comportementales et cognitives « classique ». En expérimentant la pratique de l’acteur, ce groupe visait à permettre aux participants de s’entraîner à se mettre en éveil, d’élargir leurs champs de perceptions et de compréhensions, de prendre conscience du langage du corps en dehors de la phrase verbale, de rechercher les mécanismes qui empêchent de s’affirmer, et de mettre en place des compétences qui améliorent l’affirmation de soi et augmentent ainsi les possibilités d’action. [less ▲]

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See detailEtude de la production, la lyophilisation et la conservation d’un starter lactique du lben : lait traditionnel fermenté.
Ziada, M.; Touhami, Y.; Achour, M. et al

Poster (2006, November)

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Peer Reviewed
See detailLactation effect on stable isotope ratios and mercury levels in the blood of northern elephant seals
Habran, Sarah ULg; Debier, Cathy; Lepoint, Gilles ULg et al

Poster (2006, October 27)

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios ... [more ▼]

Female northern elephant seals, Mirounga angustirostris, fast entirely during nursing while their pups may quadruple in weight over the 25-day suckling period. Stable carbon and nitrogen isotope ratios (d13C and d15N used as diet markers) and total mercury concentrations (Hg) were measured in tissues (blood cells, serum, full blood, milk and blubber) from 20 mother-pup pairs on day 4 and 21 of lactation. A systematic difference was observed between isotopic values in blood cells and serum of the mothers, linked to diet and distinct biochemical composition between blood components. d13C differed between inner and outer blubber layers and were similar between inner blubber and milk. High Hg concentrations (up to 350 ng.g-1 ww at the beginning of lactation) were found in the blood of mother elephant seals linked to their high d15N values in blood cells. Hg transfer through placenta and milk was observed between mothers and pups. A significant increase of blood Hg concentration in mothers (+285 ng.g-1ww) and a decrease in pups (-93 ng.g-1ww) were observed between days 4 and 21 of lactation. Both processes were explained by a remobilization of proteins and lipids during fasting and milk production in mothers and by a dilution of Hg in pups due to their high mass increase during this period. To conclude, physiological processes and body condition should be considered carefully when interpreting stable isotope ratios and Hg concentrations in the framework of biomonitoring. [less ▲]

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See detailAnálisis filogenético de aislamientos argentinos del virus de la leucosis bovina
Rodriguez, Sabrina ULg; Trono, K.; Jones, L.R.

Poster (2006, October 22)

ANÁLISIS FILOGENETICO DE AISLAMIENTOS ARGENTINOS DEL VIRUS DE LA LEUCOSIS BOVINA Rodríguez S.M., Trono K., Jones L.R. INTRODUCCION El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un ... [more ▼]

ANÁLISIS FILOGENETICO DE AISLAMIENTOS ARGENTINOS DEL VIRUS DE LA LEUCOSIS BOVINA Rodríguez S.M., Trono K., Jones L.R. INTRODUCCION El virus de la leucosis bovina (Bovine Leukemia Virus, BLV) es un retrovirus oncogénico B-linfocitotrópico que, junto con los virus responsables de la leucemia de células T de los humanos (Human T-Cell Lymphotropic Virus, HTLV) y su contraparte en los simios (Simian T-Lymhotropic Virus, STLV), están incluidos en el género Deltaretrovirus dentro de la familia Retroviridae. BLV es un retrovirus ampliamente distribuido entre la población bovina a nivel mundial y es el agente etiológico de la leucosis bovina enzoótica (LBE), una enfermedad crónica linfoproliferativa de ocurrencia natural en el ganado bovino que constituye la enfermedad neoplásica más frecuente del ganado bovino lechero. El estudio de la diversidad genética y la comparación de aislamientos de BLV de diferentes orígenes ha sido abordado por otros autores. Sin embargo, poco se conoce acerca de la diversidad genética en aislamientos argentinos de BLV (Vet. Microbiol. 96(1):17-23; Arch.Virol.150(3):443-458). El gen env ha sido utilizado en el estudio de relaciones filogenéticas para este virus. Una gran cantidad de secuencias de este gen, correspondientes a aislamientos de BLV de distintas áreas del mundo, se encuentran disponibles en las bases de datos públicas. OBJETIVOS Estudiar la diversidad genética y las relaciones filogenéticas de los aislamientos argentinos de BLV en relación con aislamientos de otras regiones del mundo. MATERIALES Y METODOS Se utilizaron muestras clínicas provenientes de 28 bovinos adultos naturalmente infectados con BLV provenientes de distintas zonas geográficas del país como material de partida. Se diseñaron primers y se optimizaron las condiciones de una Reacción en Cadena de la Polimerasa (PCR) para la amplificación de la totalidad de la región genómica codificante para el gen env de provirus de BLV. La extracción del DNA genómico proviral se realizó a partir de tejido tumoral y de sangre entera en los casos de animales infectados sin evidencias clínicas. Los productos de PCR obtenidos fueron purificados y secuenciados directamente. La matriz de datos fue construida incluyendo las secuencias de BLV reportadas en este trabajo y secuencias correspondientes a cepas representativas de diversas regiones geográficas. Se incorporaron en el análisis secuencias homólogas de virus relacionados, las cuales fueron utilizadas como outgroup. Las secuencias fueron alineadas mediante el programa Muscle y analizadas mediante las rutinas de Parsimonia del programa TNT. Con el fin de evaluar el soporte de los grupos identificados se llevaron a cabo análisis de Bootstrap y Jackknife. CONCLUSIONES El estudio de caracterización molecular reveló una relativa estabilidad genética para los aislamientos argentinos de BLV, apoyando el hecho de que la variación genética de estos virus complejos del grupo es menor que la de otros géneros de la familia de los retrovirus. Por otra parte, del análisis filogenético se desprende el hecho de la existencia de un agrupamiento de aislamientos representativos de la región que conforman un clado único. No se observaron clados exclusivos de regiones geográficas dentro de Argentina. [less ▲]

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See detailInmunoblot como método diagnóstico confirmatorio de la Anemia Infecciosa Equina
Álvarez, I.; Gutiérrez, G.; Vissani, A. et al

Poster (2006, October 22)

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See detailIncorporation in bread of dietary fibres from by-products of the agro-transformation.
Roiseux, Olivier; Blecker, Christophe ULg; Sindic, Marianne ULg et al

Poster (2006, October 17)

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See detailStudy of the physical changes of spray-dried inulin during storage.
Ronkart, Sébastien; Paquot, Michel ULg; Fougnies, C. et al

Poster (2006, October 17)

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See detailEffect of SK channel blockade on the firing of dorsal raphe neurons in anaesthetized rats
Alleva, Livia ULg; Rouchet, Nathalie; Waroux, Olivier ULg et al

Poster (2006, October 17)

K channels are small conductance calcium-activated potassium channels which trigger an outward current generating an afterhyperpolarization (AHP). This AHP follows a single or a train of action potential ... [more ▼]

K channels are small conductance calcium-activated potassium channels which trigger an outward current generating an afterhyperpolarization (AHP). This AHP follows a single or a train of action potential, and therefore is important in the regulation of the firing frequency and/or pattern of many types of neurons. Serotonergic (5-HT) neurons from the raphe nuclei express SK channels and exhibit a significant AHP which can be efficiently blocked in vitro by apamin and N-methyl laudanosine (NML) (Scuvée-Moreau et al, 2004). In the later study, we found that some but not all neurons (50%) had a significantly increase in their firing rate when positive current was injected after SK channel blockade. In order to determine the physiological relevance of these channels in vivo, single unit extracellular recordings were carried out in anesthetized rats and combined with iontophoresis of the specific non-peptidic SK channel blocker, UCL1684. 5-HT neurons were tested for their inhibitory response to locally applied 5-HT and histological analysis confirmed the localization of the recording site. UCL 1684 was used at a concentration of 200 µM. Out of 11 neurons recorded, 6 showed a significant increase in the production of doublets, with no effect on their mean firing rate as compared to the control condition. The other neurons were completely unaffected. These results suggest that the responsiveness of presumed 5-HT neurons to SK channel block is variable. Although the use of 200 µM UCL allow us to be sure of a sufficient SK blockade at the recording site (Waroux et al, 2005), we can not rule out the possibility that SK channels present at the dendritic level were not completely blocked. In conclusion, SK channels in vivo might play a role in controlling the firing pattern of a subgroup of 5-HT neurons. [less ▲]

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See detailIsolation of insecticidal proteins within the pea seeds (Pisum sativum L.)
Cuartero Diaz, Gaëtan; Francis, Frédéric ULg; Portetelle, Daniel ULg et al

Poster (2006, October)

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and ... [more ▼]

Consequently with the pressure exerted by chemical pesticides on environment, and the awakening of politics, the demand for bio-pesticides is increasing. Nevertheless, supply is not sufficient, and moreover those products are not competing enough. In this context, the aim of this research is to set up a biological insecticide, which is economic, with vegetal proteins resulting from alimentary industry, here the pea, Pisum sativum L.. A group of proteins, which is quite easy to highlight, is present in relatively important proportions (2%) in pea seeds, it’s the lectins class. Insecticidal effects of lectins from different organisms have already been proved. Indeed, by binding to membrane glycosyl groups of digestive tract cells, lectins can be very toxic for a lot of insects. Thus initially we focus our investigations on Pisum sativum lectins (PSL). First, PSL have been localised within the industrial process among different extraction juices. Then, a chromatography has been performed on the selected juice with FPLC technology. Although the matrix used for this chromatography, sephadex G75, is a banal bed for gel filtration, it is in this case a real combination between classical gel filtration and affinity chromatography. Indeed due the particular properties of lectins, they fixed carbonyl group of the bed and have to be eluted after the filtration part with a solution of glucose. Then the collected fractions corresponding to UV-peaks on the chromatogram were separated by electrophoresis 2D and identified by mass spectrometry (ESI MS/MS) coupled with data bank investigations. Secondly bioassays using artificial diets have been developed on Myzus persicae in the aim to study the aphicid effects of theses fractions with rm and LC50.These estimators show significant mortality rates but also change in the fecundity and in the development of nymph. [less ▲]

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See detailMelting and polymorphic behavior of binary blends made of palm oil or its fractions and other fats.
Danthine, Sabine ULg; deroanne, claude

Poster (2006, October)

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See detailXylanase production by Penicillium canescens in solid-state fermentation.
Assamoi, A.; Destain, Jacqueline ULg; Thonart, Philippe ULg

Poster (2006, October)

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See detailStudy of the effect of exposition of MCF-7/bos cells to 17-ß-estradiol by 2D DIGE
Collodoro, Mike; Dobson, Rowan ULg; Bertrand, Virginie ULg et al

Poster (2006, October)

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that ... [more ▼]

The aim of this work is to identify biomarkers following exposition to 17ß-estradiol in MCF7/bos (hormonal dependent epithelial mammary cancer) cells. The application is to optimise screening tests that will enable the detection (and eventual quantification but not identification) of numerous compounds having an estrogenic activity in a single rapid test. [less ▲]

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See detailDistribution of nerve fibres in bovine and human mucosal associated lymphoid tissues
Defaweux, Valérie ULg; Dorban, G.; Antoine, Nadine ULg et al

Poster (2006, October)

Prion cell tropism varies significantly among animal species, depending on both the agent strain and host-specific factors. For example, prions show high lymphotropism in scrapie infected sheep and vCJD ... [more ▼]

Prion cell tropism varies significantly among animal species, depending on both the agent strain and host-specific factors. For example, prions show high lymphotropism in scrapie infected sheep and vCJD, but little, if any, in sCJD or BSE. In particular, the BSE strain is associated with significant PrP-res accumulation in tonsils, spleen and appendix in humans, whereas it is largely confined to the nervous system in infected cattle. Therefore, at least in the case of BSE and vCJD, it appears that host properties can influence the accumulation of the infectious agent in lymphoid organs. Mature FDC play an important role in prion pathogenesis, since neuroinvasion following peripheral challenge is significantly impaired in their absence. The proximity between these FDC and sympathetic nerve endings is known to affect the speed of prion neuroinvasion. In this study, we analysed the mucosal innervation and the interface between nerve fibres and FDC in bovine and human tonsils and in ileal and jejunal bovine Peyer’s patches using a panel of antibodies observed by confocal microscopy. Since differences in the innervation of lymphoid organs depending on age have been reported, we analysed three categories of bovine ages (new born calves, calves less than 12 months old and bovines older than 24 months) and two categories of human ages (patients less than 5 years old and patients older than 25 years). In both species, hypothetical ways of innervation by-passing germinal centre could be postulated: nerve fibres are widely distributed in antigens/cells traffic area (the lamina propria, the interfollicular zone, the suprafollicular dome in Peyer’s patches and the lymphoepithelial area in tonsils). We pointed out that, only in ileal and jejunal Peyer’s patches and in tonsils of bovines older than 24 months, nerve fibres are observed to be in contact with FDC. In contrast, in human tonsils, no nerve fibres established contact with FDC, whatever the age. Thus, innervation of germinal centres can be said to be an age-dependent dynamic process in bovines and a weak innervation of the secondary lymphoid organs could thus be a rate-limiting step to neuroinvasion in humans. This variation could influence the way of neuroinvasion and thus, the differences of susceptibility of bovines and humans to the BSE agent. [less ▲]

Detailed reference viewed: 33 (11 ULg)