First report of indigenous canine babesiosis in Belgium

Poster (1999)

The distribution of Echinococcus multilocularis carriage in the red fox (Vulpes vulpes) in the south of Belgium

Poster (1999)

Détermination de structure et biosynthèse d'alcaloïdes défensifs de coccinelles

Poster (1999)

Il est actuellement clairement établi qu’un grand nombre de coccinelles doivent leur protection, du moins en partie, à la présence d’alcaloïdes répulsifs et parfois toxiques dans leur hémolymphe. La ... [more ▼]

Il est actuellement clairement établi qu’un grand nombre de coccinelles doivent leur protection, du moins en partie, à la présence d’alcaloïdes répulsifs et parfois toxiques dans leur hémolymphe. La plupart de ces substances sont biosynthétisées de novo ou, plus rarement, acquise à partir de leur alimentation (séquestration). Ces alcaloïdes présentent une grande diversité de structures et se rattachent à des squelettes aussi différents que ceux de l’azaphénalène, de l’homotropane, de la pipéridine, des azamacrolides… Dans le cadre de l’étude de l’évolution des mécanismes de défense chimiques de ces insectes, nous avons entrepris l’étude de la biosynthèse de deux alcaloïdes présents chez les coccinelles du genre Adalia, l’adaline et l’adalinine. Dans un premier temps, nous nous sommes proposé de déterminer la relation biogénétique existant entre ces deux alcaloïdes. Pour ce faire, nous avons synthétisé de l’adaline dont la chaîne pentyle est perdeutériée. Puis, afin d’établir l’origine polyacétate de l’adaline, nous avons mis au point un schéma de dégradation conduisant à l’isolement du carbone du carbonyle de cette molécule. Parallèlement à ces études, nous avons étendu le nombre d’espèces étudiées du point de vue de leur contenu alcaloïdique. Il s’agit de Vibidia duodecimguttata de Halizia 16-guttata et de Harmonia axyridis. Les deux premières contiennent des alcaloïdes doubles de structures complexes. Le quatrième contient majoritairement de l’harmonine. [less ▲]

Neonatal rat organ of Corti forms supernumerary hair cells in response to growth factor treatment

Poster (1999)

Powerful alternative asymmetric catalytic dihydroxylation of olefins

Poster (1998, December)

Design, synthesis and biological evaluation of sulfonylureas as original non-prostanoid thromboxane A2 receptor antagonists

Poster (1998, November 27)

Powerful alternative asymmetric Catalytic dihydroxylation of olefins

Poster (1998, November)

Alkoxyamine-mediated Controlled Radical Polymerization in Emulsion

Poster (1998, November)

Alkoxyamine-mediated Controlled Radical Polymerization in Emulsion is presented here.

A new high performance liquid chromatographic method for the quantitative analysis of hydroxy methionine analog even in difficult samples.

Poster (1998, September 13)

Oxidation des allylséléniures par les réactifs AD de Sharpless

Poster (1998, September)

Synthesis and biological evaluation of the 3-alkylamino-7-halogeno-4H-1,2,4-benzothiadiazine 1,1-dioxides: new potential KATP channels openers

Poster (1998, September)

Novel 2-oxo-2H-benzopyran derivatives as inhibitors of human leukocyte elastase

Poster (1998, September)

Preparative resolution of racemic pirlindole: chromatographic methods and determination of the absolute configuration

Poster (1998, September)

Preliminary results on the control of gonadal maturation and spawning by captive breeders of the bocachico Prochilodus magdalenae

Poster (1998, August 15)

Evaluación del Programa Nacional de Control de Calidad de Laboratorios de Diagnóstico Micológico

Poster (1998, August 12)

New specific inhibitors of human leukocyte elastase

Poster (1998, July)

Feeding of Brachionus calyciflorus on Tetrahymena pyriformis using

Poster (1998, June 22)

This study was carried out in the wider frame of the quatitative evaluation of trophic transfers occurring in large rivers between metozooplankton and protozooplankton. Since the rotifer B. calyciflorus ... [more ▼]

This study was carried out in the wider frame of the quatitative evaluation of trophic transfers occurring in large rivers between metozooplankton and protozooplankton. Since the rotifer B. calyciflorus is dominant in the river Meuse (Belgium) its feeding on the widespread ciliate T. pyriformis was studied. Grazing rates were either determined through monitoring of cell number variations in experimental media or through use of fluorescently labeled ciliates. The labeling procedure consisted in allowing the ciliates to ingest fluorescent microspheres (0.5 µm in diameter) for short period of time; since number of ingested fluorescent spheres are very stable [less ▲]

Are fasting and postload insulin good predictors of the cardiovascular risk factors?

Poster (1998, June 07)

A new type of frameshift mutation in a mitochondrial genome

Poster (1998, June)

Modification of the thermoresistance to spray-drying of a cold-adapted subtilisin by genetic engineering

Poster (1998, May 08)

Protection des pommes Jona Gold par Bacillus subtilis GA1 et S499 contre Botrytis cinerea

Poster (1998, May 08)

Molecular systematics of Arthrospira ('Spirulina') strains based on rRNA sequence analysis

Poster (1998, May)

Résolutions énantiomériques et évaluations biologiques des isomères du BPDZ 42 et du BPDZ 44

Poster (1998, May)

Genetic manipulations in Chlamydomonas reinhardtii: Use of the antisense strategy to inhibit the alternative oxidase

Poster (1998, May)

Besides the cytochrome pathway (CP), mitochondria of higher plants and many microeukaryotes possess a second electron pathway, the alternative pathway (AP), that bypasses complexes III and IV and ... [more ▼]

Besides the cytochrome pathway (CP), mitochondria of higher plants and many microeukaryotes possess a second electron pathway, the alternative pathway (AP), that bypasses complexes III and IV and catalyzes the oxidation of the ubiquinol pool by molecular oxygen. Electrons transferred by this way are not coupled to ATP production and free energy is lost as heat. The alternative oxidase (AOX) is the sole enzyme implicated in this process. Despite a great research effort during these last years, the exact role of AP is poorly understood, except in Araceae (such as Sauromatum guttatum, the ‘voodoo lily’) in which it is implicated in the thermogenesis of the inflorescence. In order to investigate the physiological role of AOX in the unicellular green alga Chlamydomonas reinhardtii, two different plasmids derived from pSP105 (Stevens et al., 1996) were constructed by placing in antisense orientation an AOX cDNA from this species under the control of the regulatory regions of the Chlamydomonas RBCS2 gene. The first plasmid possessed the full promoter, while the second one had a truncated promoter, supposed to be more efficient (Purton, personal communication). Cell wall-deficient arginine-requiring strains were co-transformed by the glass beads method with linearized pASL plasmid (complementing the arginine requirement) and linearized first or second construction. More than 1100 transformants able to grow on an arginine-free medium were screened for AOX alteration by growth tests in the presence of the two complex III inhibitors antimycin A and myxothiazol. Out of seventeen clones selected for their sensitivity to these inhibitors, only one (A1 clone) was totally unable to grow in the presence of antimycin A and myxothiazol. A PCR analysis showed that the seventeen selected clones had integrated the antisense construction. The A1 cell line was further investigated for its mitochondrial respiration activity. The cells grown under mixotrophic conditions (light + acetate as a carbon source), showed a 70% drop of the AP capacity. Moreover, the total respiration rate represented only 60% of the wild-type rate. Interestingly, despite these alterations, the A1 transformant displayed a 25% faster growth rate than the wild-type cultivated under the same mixotrophic conditions. This work was supported by grants from the Belgian FRFC (2.4527.97). Actions de recherches concertées (ARC 93-98/170) and Fonds spéciaux pour la recherche dans les universités. Reference STEVENS D.R., ROCHAIX J.D., and PURTON S. (1996), Mol. Gen. Genet. 251, 23-30 [less ▲]

Les 3-alkylamino-4H-pyrido[2,3-e]-1,2,4-thiadiazine 1,1-dioxydes structuralement apparentés au diazoxide et au pinacidil: conception, synthèse et évaluation biologique

Poster (1998, May)

Utilisation du 1,1’-thiocarbonyldiimidazole comme agent de cyclisation des aminoarylsulfonamides en vue de l’obtention d’intermédiaires utilisables dans la synthèse de nouveaux activateurs de canaux KATP

Poster (1998, May)

Les arylthiadiazinedioxydes en tant qu’activateurs des canaux potassiques: etude pharmacologique des 3-alkylamino-2-méthyl-2H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxydes

Poster (1998, May)

Normothermic preservation of rabbit hearts using a new perfluorocarbon emulsion

Poster (1998, March 07)

Séparation énantiomérique du pirlindole à l’échelle préparative

Poster (1998, January 16)

Antagonistic regulation by intact and N-terminal fragment of the human Prolactin/Growth Hormone family members : a novel mechanism for regulation of angiogenesis

Poster (1998, January)

Enantioseparation of uncharged compounds by capillary electrophoresis using mixtures of anionic and neutral -cyclodextrin derivatives

Poster (1998)

early development of the condrocranium in Chrysichtys auratus (Siluriformes, Claroteidae)

Poster (1998)

Bidirectional change of the phenotype of cerebrellar radial glial cells illustrated by the labeling in vivo and in vitro of an intermediate-filament-associated protein with the RC2 antibody

Poster (1998)

Analyse de facteurs d’environnement influençant la récolte d’embryons

Poster (1998)

Infection of undifferentiated and differentiated human nerve cells (NT2) by VZV

Poster (1998)

Mise en évidence du récepteur de la prolactine sur les leucocytes de truite arc-en-ciel (Oncorhynchus mykiss) et recherche d'un rôle fonctionnel

Poster (1998)

Fine structure of the larvae of the Myzostoma cirriferum (Myzostomida)

Poster (1998)

Adults of Myzostoma cirriferum are obligate ectocommensals that live on the surface of comatulids of the genus Antedon from the North East Atlantic and Mediterranean. M. cirriferum is a simultaneous ... [more ▼]

Adults of Myzostoma cirriferum are obligate ectocommensals that live on the surface of comatulids of the genus Antedon from the North East Atlantic and Mediterranean. M. cirriferum is a simultaneous hermaphrodite which reproduces by transferring spermatophores that penetrate the integument of the receiver myzostomes. Fertilized eggs are shed into the water column where they divide. After the embryonic stage that lasts 24h, the motile individuals enter into the larval stage. Three types of larvae are recognized in M. cirriferum: the spherical protrochophora (occuring between 24 and 48h after the laying), the pyriform trochophora (48-72h), and the pyriform, setigerous metatrochophora (72h until metamorphosis). The body of the protrochophora and trochophora is made of undifferenciated cells and ciliated cells. Two types of undifferentiated cells are present: the first contact the thin cuticle, have microvilli and include a lot of yolk droplets; the second are internal and have no or only a few yolk droplets. No basal lamina has been observed in the body of these larvae. Huge tissular modifications occur in the young metatrochophora (i.e. 96-144h after laying). The epidermis is now well underlined by a basal lamina. Two pairs of 4 chaetae lie on the sides of the larval body and a sensory caudal appendage is differentiating at its end. The digestive system is forming. It is composed of a pharynx included in an extensible introvert and a closed, internal digestive cavity. The nervous system appears and is composed of an epispheral nervous mass, a peripharyngeal collar, nervous processes and sensory cells. The anatomy of the late metatrochophora stays unchanged until metamorphosis, which generally occurs 7 days after laying. The body of the larva is made of twelve cell types. Seven are ectodermal: covering cells and ciliated cells make up most of the epidermis; primary chaetoblasts and lateral cells form the chaetal follicles; secondary chaetoblasts will build the chaetae of juveniles after metamorphosis; pharyngeal cells and myoepithelial cells make up the pharyngeal epithelium. The other cell types are neurodermal (neurons and sensory cells), endodermal (the digestive cells that border the digestive cavity) and mesodermal (parenchyma cells and muscle cells) [less ▲]

Periodontitis as potential risk factor for coronary heart disease

Poster (1998)

Sequencing the 3e9 clone from A. thaliana

Poster (1998)

Ultrastructural identification of coiled body-like structures in meristematic cells of Pisum sativum cotyledonary buds

Poster (1998)

Reproductive behaviour and daily rhythms of activity in the lesser spotted dogfish Scyliorhinus canicula (Linné, 1758).

Poster (1998)

Carrageenan gelification under H.H.P. : preparation and processing of solutions and analysis of gels obtained.

Poster (1998)

The use of Gadolinium DOTA as contrast agent for MR arthrography of the cubital joint in dogs

Poster (1998)

Struture elucidation of a neurotoxic astroglia-released factor by liquid chromatography and mass spectrometric detection

Poster (1998)

Preparation of a drug-b-cyclodextrin inclusion compound using supercritical carbon dioxide

Poster (1998)

A comparative pharmacokinetic study of intravenous solutions containig miconazole with or without cyclodextrins

Poster (1998)

Determination of l-lysine N-acetylcysteinate, its mono- and dimeric related compounds by LC-MS-MS

Poster (1998)

A comparative study of the dissolution properties of piroxicam-b-cyclodextrin inclusion complexes prepared by different methods

Poster (1998)

Facultative paedomorphosis in the Alpine newt, Triturus a. alpestris: feeding and spatial advantages of paedomorphs in an Alpine lake

Poster (1998)

Paedomorphosis in newts and salamanders is defined as the retention of larval morphology in reproductive adults. In Triturus, Paedomorphosis is facultative because some larvae transform while other retain ... [more ▼]

Paedomorphosis in newts and salamanders is defined as the retention of larval morphology in reproductive adults. In Triturus, Paedomorphosis is facultative because some larvae transform while other retain larval characteristics such as gill slits and mature as Paedomorph. This heterochronic process is thought to be an important micro- and macroevolutionary mechanism. In a population of a French alpine lake (le lac de la Cabane), Alpine newt Paedomorphs (Triturus a. alpestris) are abundant and coexist with a Metamorph minority. The coexistence of both morphs and the dominance of one on the other beg a few questions such as what ecological factors favour the paedomorph strategy. In this study, we analysed the feeding and spatial distribution of the Paedomorphs and Metamorphs of the Lac de la Cabane in June, July, August and October 1998. Newts were caught in the main four microhabitats of the lake : the shore, the bottom (3-7 meters deep), the water column and the surface, at dawn, during the day and in the evening. In this way, we caught 1481 newts to record their spatial position. We flushed the stomachs of 822 of these newts (the total prey number identified is more than 60 thousand). The feeding strategies of Metamorphs and Paedomorphs are very different : while Paedomorphs prey principally on plankton (Daphnia, Chydorus, ...), Metamorphs prey on a lot of exogenous invertebrates (flyes, aphids, ...). This characteristic is observed for males, females and juveniles. Cladocerans are also eaten by Metamorphs, but in much smaller quantity. The Paedomorph newts live in all the microhabitats of the lake while Metamorphs are hardly ever found on the deep bottom and in the water column. The spatial niche of Metamorphs is thus smaller than that of Pedomorphs. The data presented here suggest that the success of both morphs can be strongly influenced by environmental conditions, and that Paedomorphosis may be maintained and favoured by the spatial and feeding advantages of Paedomorphs into a deep lake. [less ▲]

Vizualization and structural analysis of a neurotoxic astroglia-released factor by liquid chromatography using fluorescence and mass spectrometric detection

Poster (1998)