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See detailNeural correlates of cognitive control at the item specific level in the Stroop task
Grandjean, Julien ULg; D'Ostilio, Kevin ULg; Fias, Wim et al

Poster (2010, May 04)

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See detailCharacterization of clay from northern of Morocco for their industrial application
El Ouahabi, Meriam ULg; Fagel, Nathalie ULg

Poster (2010, May 04)

Clays are a natural resource used for millennia. Currently applications such as industrial minerals are diversified. In this context, our goal is to estimate the potential of the many clay deposits in ... [more ▼]

Clays are a natural resource used for millennia. Currently applications such as industrial minerals are diversified. In this context, our goal is to estimate the potential of the many clay deposits in northern of Morocco. The choice of this region is justified by the particular abundance of clay deposits used to manufacture building materials (brick, ceramic and refractories) and pottery. This study focuses on the mineralogical, chemical and geotechnical characterization tests carried out on Tangier-Tetouan and Meknes clays from northern of Morocco. The suitability of raw clay material from those regions in order to produce ceramic and brick has not been tested yet. The results revealed that the studied samples are diversified, kaolinite and illite (Tetouan clay) and kaolinite and illite and smectite and vermiculite (Tangier and Meknes clay) based materials. There were no major differences in grain-size distribution, whereas Meknes clay was more plastic than Tetouan-Tangier clay. The cation exchange capacity show that Meknes and Tangier clay were more important than Tetouan clay. Specific surface area and thermal analaysis complete this caracterization. It was found that almost all technological properties of the Meknes clay deposit are led to the manufacture of ceramic floor tile, and Tetouan-Tangier clay provide opportunities to making brick and ceramic floor. The Tetouan- Tangier and Meknes clay are a potential ceramic raw material for growing Morrocan ceramic tile and brick industries [less ▲]

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See detailAlginate-microparticles coated with chitosan and loaded with synthetic ligands for specific removal of proteins
Petkova, E; Selina, O; Bovin, N et al

Poster (2010, May 03)

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See detailDissolved inorganic carbon dynamics and air-sea carbon dioxide fluxes during coccolithophorid blooms in the Northeast European continental margin (northern Bay of Biscay)
Suykens, Kim; Delille, Bruno ULg; Chou, Lei et al

Poster (2010, May 03)

Balch et al. (2007) evaluated global pelagic contemporary calcification from remote sensing data (mainly associated to coccolithophores) to 1.6 ± 0.3 Pg PIC yr-1 (1 Pg = 1015 g; PIC = particulate ... [more ▼]

Balch et al. (2007) evaluated global pelagic contemporary calcification from remote sensing data (mainly associated to coccolithophores) to 1.6 ± 0.3 Pg PIC yr-1 (1 Pg = 1015 g; PIC = particulate inorganic carbon). This would imply that coccolithophores would be the most important pelagic calcifier in the oceans, since other estimates of contemporary global pelagic calcification range between 0.7 Pg PIC yr-1 based on accumulation rates and sediment trap data (Milliman et al. 1999), and 1.4 Pg PIC yr-1, based on the seasonal cycle of total alkalinity (TA) in the euphotic zone (Lee 2001). The development of coccolithophorid blooms affects the seawater carbonate chemistry, and air-sea CO2 fluxes, through the organic carbon pump and the carbonate counter-pump. The ratio between calcification (carbonate counter-pump), and organic carbon production (organic carbon pump), the C:P ratio, depends on the life cycle (bloom development), and growth conditions of coccolithophores. At the onset of the coccolithophorid bloom, when nutrients are available for growth, organic carbon production dominates over calcification (C:P << 1, the so-called organic phase). At the end of the bloom, in nutrient depleted conditions, and high irradiances (due to stronger stratification), organic carbon production decreases and calcification increases (C:P ≤ 1, the so-called inorganic phase). Several manipulative experiments to test the effect of ocean acidification on coccolithophores have shown that while calcification would decrease, the export of organic carbon would increase mainly through increasing transparent exopolymer particles (TEP) production. For a credible implementation in mathematical models of such feed-back mechanisms to allow the projection of a future evolution of carbon biogeochemistry under global change, it is required to understand present day biogeochemistry and ecology of naturally occurring pelagic calcifying communities. In particular, the overall effect of phytoplankton communities on the C:P ratio, and the net effect on carbonate chemistry, and related air-sea CO2 fluxes. [less ▲]

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See detailMicrowave data and a regional climate model for studying the Greenland ice sheet surface mass balance over 1979-2009
Fettweis, Xavier ULg; Marco, Tedesco

Poster (2010, May 03)

Results (melt extent and winter accumulation) from an atmosphere-snow coupled regional climate model are compared with microwave brightness temperatures-derived estimates to study the surface mass balance ... [more ▼]

Results (melt extent and winter accumulation) from an atmosphere-snow coupled regional climate model are compared with microwave brightness temperatures-derived estimates to study the surface mass balance (SMB) changes over the Greenland ice sheet (GrIS) since 1979. Two simple algorithms are selected to retrieve the melt extent from the brightness temperatures. The first one is sensible to the production of surface meltwater as suggests the regional model and the second one is rather sensible to the presence of liquid water content into the snowpack. Both algorithms compare very well with model outputs and they are unanimous to show a significant increase of the surface melt over 1979-2009.We found also a good correlation between the March-April mean brightness temperatures and the simulated winter snow accumulation although no significant changes are found in both simulated and microwave-derived snow accumulation. The interannual variability of the brightness temperature-derived SMB components compare very well with the model results. This suggests that the variability of the model is reliable and that the model can be used to detect SMB changes over longer periods where no satellite data is available. Finally, both model and satellite agree to confirm the acceleration of the GrIS surface melting since 30 years. [less ▲]

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See detailBenthic remineralization in the northeast European continental margin (northern Bay of Biscay)
Suykens, Kim; Schmidt, Sabine; Delille, Bruno ULg et al

Poster (2010, May 02)

We report a data-set of sediment characteristics and biogeochemical fluxes at the water-sediment interface at the northeast European continental margin (northern Bay of Biscay). Cores were obtained in ... [more ▼]

We report a data-set of sediment characteristics and biogeochemical fluxes at the water-sediment interface at the northeast European continental margin (northern Bay of Biscay). Cores were obtained in June 2006, May 2007 and 2008, at 8 stations on the shelf break (120 to 180 m), and at 2 stations on the continental slope (520 m and 680 m). Sediment-water fluxes of dissolved oxygen (O2), total alkalinity (TA), nitrate (NO3-), and dissolved silicate (DSi) were measured at a total of 20 stations. Sediment characteristics include: grain size, chlorophyll-a (Chl-a) and phaeopigment (Phaeo) content, particulate organic (POC) and inorganic (PIC) carbon content, and 234Th and 210Pb activities. Sediments were sandy (fine to coarse) with organic matter (OM) (1.0 - 4.0 %) and Chl-a (0.01 - 0.95 µg g-1) contents comparable to previous publications in the same region, and a relatively high PIC fraction (0.8 - 10.2 %). Sediment-water O2 fluxes (-2.4 to -8.4 mmol O2 m-2 d-1) were low compared to other coastal environments and correlated well with OM and Chl-a content. 234Th activity profiles indicated that Chl-a sediment content (apparently the main driver of total benthic organic carbon degradation) was mainly controlled by physical mixing processes related to local hydrodynamics. The correlation between sediment-water fluxes of O2 and NO3- indicated a close coupling of nitrification/denitrification and total benthic organic carbon degradation. Dissolution of biogenic silica (0.05 to 0.95 mmol m-2 d-1) was uncoupled from organic carbon degradation, characterized by sediment-water O2 fluxes. The link between sediment-water fluxes of TA and O2 indicated metabolic driven dissolution ( 0.33 +/- 0.47 mmol m-2 d-1) of calcium carbonates (CaCO3) in the sediments which represented ~1 % of the pelagic calcification rates due to coccolithophores. These rates were below those reported in sediments of continental slopes and of the deep ocean, probably due to the high over-saturation with respect to CaCO3 of the water column overlying the continental shelf sediments of the northern Bay of Biscay. Rates of total benthic organic carbon degradation and CaCO3 dissolution were low compared to water column rates of primary production, aphotic community respiration and CaCO3 production obtained during the cruises. [less ▲]

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See detailDissolved inorganic carbon dynamics and air-sea carbon dioxide fluxes during coccolithophorid blooms in the Northeast European continental margin (northern Bay of Biscay)
Suykens, Kim; Delille, Bruno ULg; Chou, Lei et al

Poster (2010, May 02)

We present a data-set of dissolved inorganic carbon (DIC) obtained during three cruises in the northern Bay of Biscay carried out in June 2006, May 2007, and May 2008. During these cruises, blooms of ... [more ▼]

We present a data-set of dissolved inorganic carbon (DIC) obtained during three cruises in the northern Bay of Biscay carried out in June 2006, May 2007, and May 2008. During these cruises, blooms of coccolithophores occurred, as indicated by patches of high reflectance on remote sensing images, phytoplankton pigment signatures, and microscopic examinations. Total alkalinity (TA) showed a non-conservative behaviour as a function of salinity due to the cumulated effect of net community calcification (NCC) during bloom development on seawater carbonate chemistry. The cumulated impact of NCC and net community production (NCP) on DIC and the partial pressure of CO2 (pCO2) were evaluated. The decrease of DIC (and increase of pCO2) due to NCC was overwhelmingly lower than the decrease of DIC (and decrease of pCO2) due to NCP (NCC:NCP « 1). During the cruises, the northern Bay of Biscay acted as a sink of atmospheric CO2 (on average -9.7 mmol C m-2 d-1 for the 3 cruises). The overall effect of NCC in decreasing the CO2 sink during the cruises was low (on average 12% of total air-sea CO2 flux). If this is a general feature in naturally occurring phytoplankton blooms in the northern North Atlantic Ocean (where coccolithophorid blooms are the most intense and recurrent), and in the global ocean, then the potential feed-back on increasing atmospheric CO2 of the projected decrease of pelagic calcification due to thermodynamic CO2 “production” from calcification is probably minor compared to feed-backs related to changes of NCP. [less ▲]

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See detailEnfants en et hors traitements oncologiques : analyse comparative de leur qualité de vie et de leur vécu émotionnel
Missotten, Pierre ULg; Badot, Isabelle; Hoyoux, Claire et al

Poster (2010, May)

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See detailVOC emissions from a temperate mixed forest in Belgium measured by eddy-covariance
Laffineur, Quentin ULg; Heinesch, Bernard ULg; Aubinet, Marc ULg et al

Poster (2010, May)

Forest ecosystems are known to be important emitters of Biogenic Volatile Organic Compounds (BVOC). They play an important role in the atmospheric chemistry and may contribute to the formation of ozone ... [more ▼]

Forest ecosystems are known to be important emitters of Biogenic Volatile Organic Compounds (BVOC). They play an important role in the atmospheric chemistry and may contribute to the formation of ozone and aerosols with consequences on air quality and on climate. In order to better understand the effects of environmental parameters on the emissions, micrometeorological flux measurements were carried out above a mixed forest (Fagus sylvatica, Pseudotsuga menziesii, Abies alba, Picea abies) at the Vielsalm experimental site (Belgium) from July to November 2009. The flux measurements were obtained by the eddy-covariance technique using proton transfer reaction mass spectrometry. In our first measurement campaign, among other VOC compounds, isoprene (m/z 69) and monoterpenoid compounds (m/z 137) have been measured continuously with a data coverage of 75 and 58 % respectively, allowing robust statistical analysis. In our analysis, we focused on these two main emissions. A footprint analysis showed that Fagus sylvatica seems to be the main emitter of m/z 137 and Abies alba seems to be the main emitter of m/z 69. BVOCs fluxes present an exponential response to temperature. This response is more pronounced for m/z 69 while it shows the strongest seasonal evolution for m/z 137. A light dependence of m/z 69 and m/z 137 fluxes was observed but the relationship did not exhibit the same behaviour before (hyperbolic relation) and after midday (linear relation). This behaviour difference induced a hysteresis effect on the daily evolution of averaged fluxes. A robust local minimum was also observed just before midday for m/z 69 (less obvious for m/z 137) during July-August period. This minimum is not observed in the CO2 fluxes which are also measured in Vielsalm. The light dependence of BVOC emissions suggests that m/z 69 and m/z 137 emissions are directly linked to the photosynthetic cycle but the presence of the midday local minimum suggests that other processes influence the measured BVOC fluxes. As for the relation with air temperature, a seasonal evolution of flux-light dependence was far more evident for m/z 137 than for m/z 69. This seasonal variation could be explained by a phenological effect. As Fagus sylvatica is the main m/z 137 emitter, its flux is probably influenced by leaves ageing while m/z 69, as mainly emitted by Abies alba, is less influenced by needles ageing. A wind speed dependence of BVOCs fluxes was also observed, the flux increasing linearly with wind speed. This surprising result will be discussed. [less ▲]

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See detailOrganochlorine pollutants in sea turtles and their association with vitamin A
Dyc, Christelle ULg; Debier, Cathy; Thomé, Jean-Pierre ULg et al

Poster (2010, May)

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See detailStudy of the role of miR-21 in the regulation of angiogenesis
Sabatel, Céline; Malvaux, Ludovic; Bovy, Nicolas ULg et al

Poster (2010, May)

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See detailTestosterone increases cell turnover in song nucleus HVC and increases cell recruitment into Area X of adult female canaries.
Barker, Jennifer ULg; Yamamura, Takashi; Balthazart, Jacques ULg et al

Poster (2010, May)

In songbirds, song control nuclei such as HVC and Area X, show seasonal changes in volume that are regulated, at least in part, by the action of gonadal testosterone (T) and its metabolites. These changes ... [more ▼]

In songbirds, song control nuclei such as HVC and Area X, show seasonal changes in volume that are regulated, at least in part, by the action of gonadal testosterone (T) and its metabolites. These changes in volume are a result of changes in cell size, dendritic branching and, in HVC, the incorporation of newborn neurons. Doublecortin (DCX) is a microtubule-associated protein expressed during development and in adulthood in post-mitotic migrating and differentiating neurons in mammals. Our previous studies in male canaries demonstrated that DCX is expressed in BrdU-positive neurons consistent with DCX being a marker of neurogenesis in adult canaries. Testosterone induces marked increases in song nuclei volume in adult female canaries making these nuclei more male-like. Within the songbird brain, T can be metabolized to 5 alpha-dihydrotestosterone (DHT) and 17 beta-estradiol (E2). We found previously that both these metabolites are required to increase the volume of song nuclei in adult female canaries, but the cellular basis of this adult neuroplasticity is not well understood. Within HVC, the number of DCX-immunoreactive (ir) cells can be increased by photostimulation or treatment with T, but the effects of T and its metabolites on cell death in the songbird brain had not yet been elucidated. We therefore examined the effect of DHT and E2 on DCX expression and cell death in the song nuclei of adult female canaries. Intact female canaries were implanted with Silastic tubing containing crystalline T, DHT, E2, or a combination of DHT+E2. Control animals received empty implants. All birds were kept under early spring-like photoperiodic conditions (11L:13D) for 3 weeks. In HVC, the total number of DCX-ir cells was increased by treatment with T or DHT+E2 as compared to control birds, but was not affected by treatment with DHT or E2 alone. The number of pyknotic cells observed in the HVC was also increased by T but not by its metabolites. In Area X, the total number of DCX-ir cells was increased by treatment with T or DHT+E2, but the number of pyknotic cells was unaffected by hormone treatment. These results suggest that T enhances cellular turnover in the HVC (migration into, and cell death within, HVC), but affects only recruitment of new neurons into Area X. [less ▲]

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See detailL’Inventaire Systémique de Qualité de Vie en oncologie pédiatrique : développement d’un module spécifique
Missotten, Pierre ULg; Hoyoux, Claire; Dupuis, Gilles et al

Poster (2010, May)

Introduction Dans la littérature pédiatrique relative à l’évaluation de la qualité de vie et de la qualité de vie liée à la santé, deux types bien distincts d’outils existent: les outils génériques et les ... [more ▼]

Introduction Dans la littérature pédiatrique relative à l’évaluation de la qualité de vie et de la qualité de vie liée à la santé, deux types bien distincts d’outils existent: les outils génériques et les outils spécifiques. Les mesures génériques s’adressent à tout enfant (Rodary, 2000). Elles permettent d’établir des comparaisons entre différents groupes d’enfants (malades et sains) mais peuvent ne pas être sensibles ni aux changements mineurs dans le fonctionnement d’un enfant ni aux aspects spécifiques de la maladie qui sont pourtant cliniquement pertinents dans sa vie (Harding, 2001; Patrick & Erikson, 1993; Raat et al., 2006). Les mesures spécifiques concernent les enfants atteints d’une maladie donnée: asthme, diabète, cancer,… (Rodary, 2000). Elles sont plus sensibles aux implications des différents traitements et sont probablement plus appropriées tant pour évaluer les interventions que pour comparer l’impact de traitements alternatifs (Eiser, 1997). Par contre, elles ne permettent pas de comparer la qualité de vie d’enfants et d’adolescents souffrant de maladies différentes (Levi & Drotar, 1998). De plus, leur spécificité s’oppose à la conception de la qualité de vie en tant que construction applicable à tous les enfants, les standards de qualité de vie étant universels (Wallander et al., 2001). Pour contrer ces inconvénients respectifs, le développement d’un troisième type d’outil -l’outil modulaire- est actuellement encouragé. Ce dernier se compose d’une partie générique à laquelle vient s’ajouter une partie spécifique à la pathologie. Objectif L’objectif de cette communication est de présenter le développement d’un module spécifique « cancer » se rattachant à la partie générique de l’Inventaire Systémique de Qualité de Vie pour Enfants (ISQV-E© ; Missotten, Etienne et Dupuis, 2007) et Adolescents (ISQV-Ados© ; Missotten, 2007). L’ISQV-E© et l’ISQV-Ados© sont des auto-questionnaires, respectivement destinés aux enfants de 8 à 12 ans et aux adolescents de 13 à 18 ans. Ils se différencient tant au niveau du contenu de certains items qu’au niveau du matériel proposé. Toutefois, tous deux évaluent dans quelle mesure chaque enfant ou adolescent parvient à atteindre les buts qu’il se fixe dans différents domaines de sa vie. Ces derniers recouvrent principalement le fonctionnement physique, le fonctionnement émotionnel, le fonctionnement social et familial et le fonctionnement cognitif. La spécificité de ces questionnaires tient à leur évaluation de la qualité de vie en termes d’écart entre la situation actuelle du jeune et son attente (but) et à leur prise en compte des capacités d’adaptation des enfants et adolescents. Méthode L’élaboration du module spécifique venant s’ajouter aux items génériques a été effectuée sur base de deux types d’informations: - discussions multidisciplinaires réunissant l’ensemble de l’équipe d’hémato-oncologie pédiatrique du CHR de la Citadelle. - recensement des outils spécifiques existant en oncologie pédiatrique et du seul outil modulaire ayant un module cancer : le Pediatric Quality of Life Inventory (Varni et al., 2002). Résultats Sur base de ces informations, 6 items spécifiques ont été générés: nausées et vomissements, fonctionnement physique, anxiété liée aux traitements, connaissance de la maladie, des traitements et de leurs effets, relations avec le personnel soignant et fonctionnement intellectuel. Ces 6 items spécifiques sont appréhendés, de manière similaire aux items génériques, et donc de manière différente chez l’enfant et l’adolescent. [less ▲]

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See detailFalse memories and hemispheric processing in aging
Gajewski, Celine; Dehon, Hedwige ULg; Peigneux, Philippe ULg et al

Poster (2010, May)

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See detailUtilisation optimisée de GATE sur un cluster Sun Grid Engine
Taleb, Dounia ULg; Seret, Alain ULg

Poster (2010, May)

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See detailTransport and interaction blockade of cold bosonic atoms in a triple-well potential
Schlagheck, Peter ULg; Malet, Francesc; Cremon, Jonas C. et al

Poster (2010, May)

We investigate the transport properties of cold bosonic atoms in a triple-well potential that consists of two large outer wells, which act as microscopic source and drain reservoirs, and a small inner ... [more ▼]

We investigate the transport properties of cold bosonic atoms in a triple-well potential that consists of two large outer wells, which act as microscopic source and drain reservoirs, and a small inner well, which represents a quantum-dot-like scattering region. Such configurations can be realized by optical triple-well lattices generalizing the setup realized in the interaction blockade experiment by Cheinet et al. [Phys. Rev. Lett. 101, 090404 (2008)]. Bias and gate "voltages" are introduced in order, respectively, to tilt the triple-well configuration and to shift the energetic level of the inner well with respect to the outer ones. By means of exact diagonalization considering a total number of 6 atoms in the triple-well potential, we find diamond-like structures for the occurrence of single-atom transport in the parameter space spanned by the bias and gate voltages, in close analogy with the Coulomb blockade in electronic quantum dots. We demonstrate how one can infer the interaction energy in the central well from the distance between the diamonds, and discuss the possibility of realizing single-atom pumping across the quantum dot. [less ▲]

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See detailGlass production in Merovingian time
Van Wersch, Line ULg; Mathis, François ULg; Othmane, G. et al

Poster (2010, May)

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See detailβ3 integrin sequence variation and rodent susceptibility to hantaviruses
Pagès, Marie ULg; Tatard, Caroline; Galan, Maxime et al

Poster (2010, May)

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See detailEfficient access pricing and endogenous market structure
Gautier, Axel ULg

Poster (2010, May)

We investigate how a regulatory mechanism can influence the nature of competition in a network industry. In the downstream market, the seller of a differentiated retail product competes with an incumbent ... [more ▼]

We investigate how a regulatory mechanism can influence the nature of competition in a network industry. In the downstream market, the seller of a differentiated retail product competes with an incumbent firm. The incumbent firm is also the owner of the essential input. The regulator may or may not observe the cost of the entrant. To maximize social welfare the regulator specifies the access charge that the entrant must pay to the incumbent, and the retail prices. The optimal access charge is a uniform price that respects the criteria of transparency and non-discrimination that are imposed by the competition and regulation directives in most countries. We derive new formulas for retail and access prices adhering to the traditional Ramsey rule. Since the competing firm takes the decision to enter following the choice of the regulatory regime, the nature of the retail market competition is endogenous [less ▲]

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See detailUpdating hydrogen fluoride (HF) FTIR time series above Jungfraujoch: comparison of two retrieval algorithms and impact of line shape models
Duchatelet, Pierre ULg; Demoulin, Philippe ULg; Hase, Frank et al

Poster (2010, May)

Fluorine enters the stratosphere mainly in the form of chlorofluorocarbons (CFCs; principally CFC-11 and CFC-12) and tetrafluoromethane (CF4), which have been widely emitted at ground level by human ... [more ▼]

Fluorine enters the stratosphere mainly in the form of chlorofluorocarbons (CFCs; principally CFC-11 and CFC-12) and tetrafluoromethane (CF4), which have been widely emitted at ground level by human activities over the past few decades. In the lower stratosphere, the photolysis of the long-lived CFC-11 (CCl3F) and CFC-12 (CCl2F2), whose emissions from the ground have been progressively phased out by the Montreal Protocol and its successive Amendments and Adjustments, leads to the formation of the two reservoirs: COClF and COF2. Subsequent photolysis of these two compounds then liberates F atoms, which could quickly react with CH4, H2O or H2 to form the extreme stable hydrogen fluoride (HF) gas. The formation of HF by these reactions is significant, as they make HF the largest fluorine reservoir in the middle and upper stratosphere. Despite the fact that fluorine does not directly participate in ozone depletion, measurements of the concentration of individual F-containing species at different altitude of the atmosphere are important as they reflect the amounts of anthropogenic gases – which also often bear ozone-threatening Cl atoms - transported into the middle atmosphere as well as their decomposition. Since the first detection of hydrogen fluoride in the Earth’s atmosphere by Zander (1975), several studies dealing with HF total column amounts derived from ground-based Fourier transform infrared (FTIR) observations at several latitudes in both hemispheres have been published. In addition, these last years have seen the emergence of more sophisticated retrieval algorithms (e.g. SFIT-2, PROFFIT) allowing to inverse total or partial columns as well as vertical distribution of the target gas from ground-based FTIR spectra. In this contribution, we propose to compare HF total columns derived from FTIR high-resolution ground-based observations performed at the Jungfraujoch (46.5°N, 8.0°E, 3580 m asl) by using two different retrieval codes: SFIT-2 v.3.91 and PROFFIT v.9.5. The impact of spectroscopic parameters (Voigt line shape model versus Galatry model line shape) on HF retrievals is also analyzed. References: Zander, R.: Présence de HF dans la stratosphère supérieure, C.R. Acad. Sci. Paris. Série B., 281, 213-214, 1975. [less ▲]

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See detailInfluence de l'ajout de co-solvant pour l'estérification du D-mannose et de l'acide D-glucuronique assistée par les lipases
Richard, Gaetan ULg; Brognaux, Alison ULg; Nott, Katherine ULg et al

Poster (2010, May)

En parallèle à la production de biocarburant, le second objectif du bioraffinage est la transformation des sucres issus de l’hydrolyse de la lignocellulose en produits d’intérêt, qui se substitueront aux ... [more ▼]

En parallèle à la production de biocarburant, le second objectif du bioraffinage est la transformation des sucres issus de l’hydrolyse de la lignocellulose en produits d’intérêt, qui se substitueront aux produits de la vie courante issus du pétrole. L’une des voies de valorisation envisageable est la synthèse de molécules amphiphiles par greffage de chaînes grasses sur le sucre. Ces composés trouveraient alors des applications dans de très nombreux domaines utilisant les tensioactifs (alimentaire, détergence, peinture,…) . Dans le cadre du programme d’excellence TECHNOSE et du projet d’Action de Recherche Concertées SUPERZYM, deux sucres ont été testés : un polyol, le D-mannose, et un sucre acide, l’acide D-glucuronique. Afin de conférer un caractère amphiphile à la molécule, les lipases ont été utilisées en tant qu’outils d’estérification. Ces triglycérides hydrolases sont en effet capables de catalyser ce type de réaction, et de nombreux exemples dans la littérature démontrent le fort potentiel industriel de ces enzymes , . L’estérification du D-mannose et de l’acide D-glucuronique a donc été mise en œuvre en présence de la lipase de Candida antarctica B (Novozyme 435). La réaction est effective, mais l’ajout de co-solvants tels que la pyridine ou le DMSO améliore les vitesses initiales et les rendements de la réaction. Ces améliorations seront discutées et comparées sur ce poster. [less ▲]

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See detailOntogeny of sex differences in steroid-sensitive regions in the quail brain (Coturnix Japonica)
Mouriec, Karen ULg; Bardet, Sylvia; Balthazart, Jacques ULg

Poster (2010, May)

Sex differences affecting the expression of sexual behavior are observed in many species. In quail, expression of the male-typical copulatory pattern is androgen-dependent. This behavior disappears within ... [more ▼]

Sex differences affecting the expression of sexual behavior are observed in many species. In quail, expression of the male-typical copulatory pattern is androgen-dependent. This behavior disappears within a week after castration and is restored after a few days of treatment with exogenous testosterone. In contrast, ovariectomized females treated with testosterone never show the sequence of male-typical copulatory behavior. This sex difference in responsiveness to testosterone results from organizational effects of embryonic estrogens secreted by the female ovary. The behavioral phenotype can be completely reversed by treatment, before embryonic day 12, of male embryos with estrogens or of female embryos with an aromatase inhibitor. In the quail brain, the medial preoptic nucleus (POM) is a necessary and sufficient site for the activation by testosterone of sexual behavior. Aromatase, the enzyme converting testosterone into estradiol, is densely expressed in POM and its activity is sexually differentiated (males>females) even when birds are treated with a same dose of testosterone. Aromatase and other neuroendocrine systems are thus, like sexual behavior, differentially activated by testosterone in adult quail but the cellular basis of these sexually differentiated features presumably organized in early life by steroid action have not been identified. To analyze the ontogeny of steroid sensitive regions that control behavioral sex differences in the quail brain, we injected 5-bromo-2-deoxyuridine (BrdU) in eggs at different stages of the embryonic (E) development (E8, E10, E12, E14 and E16) and sacrificed the animals at postnatal (PN) day 56. Large numbers of BrdU-positive cells were observed throughout the POM of males and females injected on E8-E10 but most cells were post-mitotic in both sexes on E14-E16. E12 injections resulted in a larger number of BrdU cells in females than in males. This differential number of BrdU-positive cells seen at PN56 in birds injected on E12 could result from a) a difference in the age at which cells become post-mitotic (males before females or alternatively females before males, so that male cells labeled by BrdU on E12 dilute their label in subsequent divisions) or b) a differential apoptosis between E13 and PN56. However, no sex differences in the number of BrdU positive cells was observed in embryos injected with BrdU on E12 and killed on E13. Furthermore, BrdU injections on E14 labeled very few cells at PN 56 suggesting that the POM is essentially post-mitotic at that age. The sex difference observed in birds injected at E12 should result from a differential apoptosis after E13. Double-label immunohistochemistry for BrdU and the neuronal marker Hu (C/D) indicated that all BrdU-positive cells born between E8 and E16 are not neurons (no double label) suggesting that these are glial cells. This sex difference in (glial?) proliferation around the end of the critical period of sexual differentiation may play a key role in the differentiation of brain and behavior. The specific phenotype of these cells and the mechanisms mediating their differential development are currently under investigation. [less ▲]

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See detailEvaluation du potentiel de récupération d’énergie à l’échappement d’un moteur TDI à l’aide d’un cycle de Rankine organique (ORC)
Declaye, Sébastien ULg; Quoilin, Sylvain ULg; Lemort, Vincent ULg

Poster (2010, May)

Une étude expérimentale a été menée sur un cycle de Rankine organique de faible puissance (2,5kW). Les résultats expérimentaux ont permis de calibrer un modèle du cycle qui a ensuite été utilisé afin ... [more ▼]

Une étude expérimentale a été menée sur un cycle de Rankine organique de faible puissance (2,5kW). Les résultats expérimentaux ont permis de calibrer un modèle du cycle qui a ensuite été utilisé afin d’évaluer le potentiel de récupération d’énergie à l’échappement d’un moteur TDI pour 13 points de fonctionnement représentatifs. Les résultats montrent la possibilité de produire jusqu’à 2,4kW de puissance mécanique additionnelle. La fraction d’énergie récupérée (ERR) pouvant atteindre 6,8% de la puissance à l’arbre moteur. [less ▲]

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See detailResponse of the European ecosystems to climate change: a modelling approach for the 21st century
Dury, Marie ULg; Warnant, Pierre; François, Louis ULg et al

Poster (2010, May)

According to projections, over the 21st century, significant climatic changes appear and will be strengthened all over the world with the continuing increase of the atmospheric CO2 level. Climate will be ... [more ▼]

According to projections, over the 21st century, significant climatic changes appear and will be strengthened all over the world with the continuing increase of the atmospheric CO2 level. Climate will be generally warmer with notably changes in the seasonality and in the precipitation regime. These changes will have major impacts on the environment and on the biodiversity of natural ecosystems. Geographic distribution of ecosystems may be modified since species will be driven to migrate towards more suitable areas (e. g., shifting of the arctic trees lines). The CARAIB dynamic vegetation model (Carbon Assimilation in the Biosphere) forced with 21st century climate scenarios of the IPCC (ARPEGE-Climat model) is used to illustrate and analyse the potential impacts of climate change on tree species distribution and productivity over Europe. Changes in hydrological budget (e. g., runoff) and fire effects on forests will also be shown. Transient runs (1975-2100) with a new dynamic module introduced in CARAIB are performed to follow the future evolutions. In the new module, the processes of species establishment, competition and mortality due to stresses and disturbances have been improved. Among others, increased atmospheric CO2 and warmer climate increase tree productivity while drier conditions decrease it. Regions with more severe droughts will also be affected by an increase of wildfire frequency, which may have large impacts on vegetation density and distribution. [less ▲]

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See detailLes inventaires forestiers par échantillonnage comme outil d’aide à la gestion : exemple d’application au Domaine royal de Ciergnon
Gheysen, Thibaut ULg; Arnould, Dominique; Nachtergaele, Quentin et al

Poster (2010, May)

Ce poster présente un exemple concret de mise en oeuvre d’inventaire forestier de gestion sur un massif d’environ 5000 ha de forêt situé en Wallonie. L’objectif est de fournir au gestionnaire un tableau ... [more ▼]

Ce poster présente un exemple concret de mise en oeuvre d’inventaire forestier de gestion sur un massif d’environ 5000 ha de forêt situé en Wallonie. L’objectif est de fournir au gestionnaire un tableau de bord rassemblant les principaux indicateurs décrivant l’état de son patrimoine forestier. Le plan d’échantillonnage est constitué d’une grille systématique comportant 1 point de sondage par 4 ha. Les points sont permanents et visités tous les 4 ans. Les informations, délivrées dans un rapport d’inventaire standardisé, concernent notamment : la surface terrière et le volume par ha, la structure, la composition, l’état de la régénération, le potentiel biomasse, la diversité spécifique et les accroissements. [less ▲]

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See detailInventaire sur l’affectation des terres de la Belgique dans le cadre du Protocole de Kyoto et de la CCNUCC
Bauwens, Sébastien ULg; Hebert, Jacques ULg; Lejeune, Philippe ULg

Poster (2010, May)

La Belgique réalise annuellement un inventaire de ses émissions de gaz à effet de serre (GES) dans le cadre du Protocole de Kyoto et de la Convention Cadre des Nations Unies sur les Changements ... [more ▼]

La Belgique réalise annuellement un inventaire de ses émissions de gaz à effet de serre (GES) dans le cadre du Protocole de Kyoto et de la Convention Cadre des Nations Unies sur les Changements Climatiques (CCNUCC/UNFCCC). L’affectation des terres et le changement d’affectation des terres (LULUCF) forment l’un des cinq principaux secteurs qui doivent être rapportés dans cet inventaire national. Ce poster présente la méthode mise en œuvre au niveau de la Belgique pour réaliser le rapportage de ce secteur, ainsi que les résultats pour 1990 et 2008. [less ▲]

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See detailMicrowave – assisted synthesis of carbohydrate compounds, focus on uronic acid derivatives.
Laurent, Pascal ULg; Richel, Aurore ULg; Wathelet, Bernard ULg et al

Poster (2010, May)

At the moment, biorefining is increasingly seen as a promising alternative to petrochemical sector since it targets both the replacement of part of petroleum as a source of energy and the development of ... [more ▼]

At the moment, biorefining is increasingly seen as a promising alternative to petrochemical sector since it targets both the replacement of part of petroleum as a source of energy and the development of chemicals from the biomass, such as detergents, phytopharmaceutics, solvents, plastics, etc. The valorisation of carbohydrates arising from the hydrolysis of renewable feedstocks is therefore an area of outmost interest. In this context, uronic acids such as glucuronic acid (GlcA) or galacturonic acid (GalA) derived from widely available raw material such as hemicellulose or pectins represent important biocompatible and bioresorbable starting material. The quest of highly effective, environmentally friendly and straightforward chemical strategies to transform totally O-unprotected uronic acids into high valuable materials remains actually a particularly challenging task. A new strategy enabling the quantitative “one-pot” production of water-soluble monosubstituted D-glucofuranosidurono-6,3-lactones from unprotected D-glucuronic acid (D-GlcA) involving microwaves and an inexpensive siliceous-based promoter will be described. The use of a heterogeneous acid catalytic systems consisting of sulfuric acid impregnated onto silica (H2SO4/SiG60) or onto carbon (H2SO4/C), offers a green alternative to unrecyclable liquid sulfuric acid and permit the developpment of a truly eco-friendly green process, as these supported acids were readily separated from liquid products, without neutralization, by decantation or filtration, thus minimizing energy consumption and wastes. Faced with environmental concerns, this solventless methodology offers attractive features, including short reaction times, high yields and easy set-up and workup. [less ▲]

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See detailHigh-resolution measurements and modelling of the Cape Ghir upwelling filament during the CAIBEX survey
Troupin, Charles ULg; Sangrà, Pablo; Arístegui, Javier et al

Poster (2010, April 26)

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See detailDecadal changes of carbon dioxide in the Southern North Sea
Harlay, Jérôme ULg; Delille, Bruno ULg; Borges, Alberto ULg

Poster (2010, April 26)

Since late 2000, we have acquired partial pressure of CO2 (pCO2) data underway with an equilibrator coupled to an infra-red gas analyser on all the cruises carried out on RV Belgica. Here, we discuss the ... [more ▼]

Since late 2000, we have acquired partial pressure of CO2 (pCO2) data underway with an equilibrator coupled to an infra-red gas analyser on all the cruises carried out on RV Belgica. Here, we discuss the decadal changes of pCO2 during winter-time in the Southern North Sea. The trends are faster than those reported in open oceanic waters, although strongly modulated by inter-annual variability that seems to be related to the North Atlantic Oscillation. [less ▲]

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See detailAnnular Groove Phase Mask coronagraph in diamond for mid-IR wavelengths
Delacroix, Christian ULg; Mawet, Dimitri; Surdej, Jean ULg et al

Poster (2010, April 25)

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See detailDevelopment of an analytical method to determine the cryolitic bath composition by Raman spectroscopy
Malherbe, Cédric ULg; Gilbert, Bernard ULg

Poster (2010, April 23)

Since the end of the nineteenth century, metallic aluminium is produced by electro-deposition from a solution of aluminium oxide in cryolitic melts around 1000°C (Hall-Héroult process). The industrial ... [more ▼]

Since the end of the nineteenth century, metallic aluminium is produced by electro-deposition from a solution of aluminium oxide in cryolitic melts around 1000°C (Hall-Héroult process). The industrial melt is composed mostly of cryolite (Na3AlF6) and AlF3 and is characterized by the molar NaF/AlF3 ratio, named cryolitic ratio (CR). It turns out that the bath composition is critical: for instance, it has been shown that a small change in the Al2O3 content leads to a great change in the overvoltage required for the electrolysis. Therefore controlling the melt composition is very important in order to reduce the energy lost. Unfortunately no in situ analytical method allows studying the composition of the melt yet. Considering our experience in the study of such highly corrosive media by Raman spectroscopy and since the bath spectrum is function of both the CR and the Al2O3 content, we have proposed in the past to apply that technique to the direct determination of the melt composition. Despite the CR could be well evaluated in the lab, experimental problems however made the practical application difficult. The purpose of this presentation will be to show the new results obtained on an updated instrument: - Spectra are recorded in 20 s or less with a higher quality than before. - The previously developed home-made software was adapted to the updated instrument and various spectra analysis procedures are under study. - A procedure to prepare reference samples was also developed taking into account the homogeneity problems that have been met. - The slopes of the alumina calibration curves are depending on the bath CR, in confirmation of our previous results. - The new results are compared with the previous ones. It will be concluded that Raman spectroscopy is indeed becoming a suitable technique for developing an analytical method to determine the composition of industrial cryolitic melts. [less ▲]

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See detailA regional flux-based risk assessment approach of contaminated sites on groundwater bodies
Brouyère, Serge ULg; Jamin, Pierre ULg; Dollé, Fabien ULg et al

Poster (2010, April 22)

In the context of the water framework directive, management plans have to be set up about water quality in surface and groundwater bodies in the EU. One of the first steps is to evaluate the risk of ... [more ▼]

In the context of the water framework directive, management plans have to be set up about water quality in surface and groundwater bodies in the EU. One of the first steps is to evaluate the risk of contamination of these water resources, and particularly the risk posed by contaminated industrial sites. From the perspective of water resource management, each of these sites taken individually does not necessary constitute a major threat. However, in heavily industrialised and urbanised areas, the cumulative effect of multiple contaminant sources is likely to present a risk. In order to propose adequate but still economically reliable measures, the calculated risk should be based on a so-called megasite approaches using criteria reflecting the water quality deterioration, in a cumulative way, at the scale of the whole surface water or groundwater body. A GIS-based regional risk assessment approach is developed here for groundwater bodies using the SEQ-ESO currently used within the Walloon Region as indicator to reflect the quality status of the groundwater body. The approach is applied on the groundwater body RWM073 “Gravels and alluvial deposits of the Meuse river between Engis and Herstal”, identified at risk of not reaching a good quality status by 2015. The different steps of this methodology consist of an inventory of proved or potential contaminating industrial sites, a numerical modelling of pollutants behaviour at the scale of the groundwater body and the application of the SEQ-ESO that finally gives a global quality status of the whole groundwater body. This analysis also serves as basis for a socio-economic approach intending to provide indications on costs and benefits generated by total or partial remediation of the contaminated groundwater bodies according to the different management scenarios. [less ▲]

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See detailPhysically-based groundwater vulnerability assessment for groundwater protection and land-use management
Popescu, Ileana-Cristina; Brouyère, Serge ULg; Derouane, Johan et al

Poster (2010, April 22)

Numerous groundwater vulnerability and risk mapping techniques have been developed taking into consideration a variable number of factors. Most common techniques produce maps of indexes based on a ... [more ▼]

Numerous groundwater vulnerability and risk mapping techniques have been developed taking into consideration a variable number of factors. Most common techniques produce maps of indexes based on a relatively empirical combination of weighted factors reflecting the protective effect of underground formations overlying the groundwater resource. The limitation of such methods is related to their use of a qualitative definition of groundwater vulnerability, as opposed to a definition based on a quantitative description of contaminant migration. A physically-based point of view and definition of the vulnerability is proposed and based on three factors describing a pollution event, which are the contaminant transfer time from the hazard location to the 'target', the contamination duration at the 'target' and the level of contaminant concentration reached at the 'target'. This concept allows a clear distinction between conventional aspects and physically-based results in the building of a final vulnerability indicator. This methodology has the further advantage to consider the possible impact of runoff conditions occurring at the land surface and possibly leading to lateral contamination of groundwater through downstream preferential infiltration features, such as karstic features. Practically, this method needs to describe and simulate the pollutant migration in the unsaturated zone and possibly in the saturated zone in order to assess the breakthrough curve at the 'target'. Preliminary application is illustrated on a case-study located in a Néblon limestone basin in Belgium, one of the main groundwater resources for the city of Liège in the Meuse basin. Perspectives are proposed towards a generalisation of the vulnerability concept for risk assessment within a pressure - state - impact framework. [less ▲]

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See detailHow Climate Change Could Affect The Hydrology In Walloon Region ?
Bauwens, Alexandra ULg; Sohier, Catherine ULg; Degre, Aurore ULg

Poster (2010, April 22)

Research on climate change and its impacts on hydrology are increasingly important nowadays. AMICE project focuses on the adaptation of the Meuse to the impacts of climate change. On this study, we focus ... [more ▼]

Research on climate change and its impacts on hydrology are increasingly important nowadays. AMICE project focuses on the adaptation of the Meuse to the impacts of climate change. On this study, we focus on the Walloon tributaries of the Meuse river, and more specifically on the Vesdre and the Lesse sub-catchments. Climate change scenarios are outputs of the CCI-HYDR Perturbation Tool for time slice 2020-2050 and 2070-2100. This tool allows us to build climate change time series and to use it as input of our hydrological models. The most pessimistic and the most optimistic scenarios are selected. The hydrological model used is called EPICGrid and it is a physically based distributed model at catchment scale. EPICGrid used the perturbed meteorological data to provide hydrograms for the different scenarios and time slice for the Vesdre and the Lesse sub-catchments. It appears that for the Vesdre daily discharge with a return period of 100 years (Qd100) could varies between -3% and +27% when the mean annual 7-day Minimum flow with a return period of 50 years (MAM750) varies between -37% and +16% for 2070-2100. For the Lesse, Qd100 varies between -30% and +50% when MAM750 varies between -21% and +28% for 2070-2100. The broad range of discharge variations reflects the broad range of meteorological variation. These results will be used further in the AMICE project to build an adaptation strategy. [less ▲]

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See detailFlood Protection Design Based on Micro-scale Risk Modelling
Ernst, Julien ULg; Dewals, Benjamin ULg; Detrembleur, Sylvain ULg et al

Poster (2010, April 22)

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See detailNovel Relative ICPL Based Quantitative Phospho- and Glycoproteome Analysis Method
Fleron, Maximilien ULg; Greffe, Yannick ULg; Massart, Anne-Cécile ULg et al

Poster (2010, April 16)

Large scale proteomic analysis remains challenging partially because proteins are inhomogeneous and often influenced by a variety of structural modifications. In particular, these specific chemical ... [more ▼]

Large scale proteomic analysis remains challenging partially because proteins are inhomogeneous and often influenced by a variety of structural modifications. In particular, these specific chemical modifications called posttranslational modifications (PTM) are crucial determinants for the protein function and biological role. Up to now there have been a growing number of studies describing the enrichment and identification of PTM. However, a significant dearth of data offering a reliable methodology for PTM quantification does exist. The present work aims at developing a label based protein PTM quantification strategy and demonstrating its value on comparative analysis of cells originating from two distinct prostate metastasis sites. PC3 and LNCaP cells isolated from bone and lymph node prostate cancer metastasis sites respectively, were lysed and spiked with three non-human proteins serving as internal standards. Following this, the samples were reduced and alkylated, digested with trypsin and subjected to peptide ICPL (isotope coded protein label) labeling. The two peptide containing samples were joined together followed by the affinity isolation of phospho- (using TiO2 metal affinity chromatography) and glycopeptides (oxidized glycans were bound on hydrazide resin). The enriched fraction as well as the flow-through were analyzed on a 2D-(SCX and C18-RP)-nano-HPLC system. The peptide identification and quantification was conducted using electrospray ion-trap mass spectrometer (Bruker, HCT-ultra). Validation of the differentially modulated proteins was conducted in several biological and technical replicates using the label free MSe based quantification strategy. This PTM based, novel relative protein quantification using post-digest ICPL has detected over 598 individual proteins. Of these more than 95 % have been successfully quantified. PTM enrichment methodologies allowed an isolation rate of 91 % and 50 % for phosphorylated and glycosylated proteins respectively. The detailed comparison of PC3 and LNCaP cells has shown specific overexpression of selected proteins indicating differences between these two prostate metastatic cell lines. Several of these modulated proteins have been previously described to be related to prostate cancer (e.g. annexin A2 and vimentin) while others could be considered as potentially novel. These proteins might be implicated in the fundamental process related to metastasis dissemination. However, because of the known discrepancy between cell systems and clinical material, the present study can be regarded only as a step towards elucidation of these complex interactions. [less ▲]

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See detailMALDI-TOF/TOF sequencing of peptide toxins from animal venoms
Quinton, Loïc ULg; Echterbille, Julien ULg; Gilles, Nicolas et al

Poster (2010, April 16)

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See detailMALDI MS Tissue Imaging of Crystallins using an original metyhod to direct protein identification on lens slices
Bertrand, Virginie ULg; Debois, Delphine ULg; Quinton, Loïc ULg et al

Poster (2010, April 16)

The lens is a transparent, biconvex structure in the eye that, along with the cornea, helps to refract light to be focused on the retina. Crystallins, α, β and γ, are the predominant structural proteins ... [more ▼]

The lens is a transparent, biconvex structure in the eye that, along with the cornea, helps to refract light to be focused on the retina. Crystallins, α, β and γ, are the predominant structural proteins in lens. They constitute 90% of water soluble proteins and contribute to its transparency and refractive properties by a uniform concentration gradient in the lens. Nevertheless, if these crystallins undergo post translational modifications, they become less soluble and the opacity of eye lens increases. This phenomenon defines cataract. Yet, the nature and the mechanism of occurring of these modifications and how they happen are not fully understood. MALDI mass spectrometry imaging is a recent technique allowing examining proteins in their native location without the need for traditional processing methods such as extraction, homogenization, and separation. Nevertheless, one main difficulty lies in the identification of the detected species, especially proteins. MALDI-In Source Decay (MALDI-ISD) is a fragmentation process occurring in the mass spectrometer ion source. When the analyzed sample is a protein, ISD fragmentation leads to b-, c- and z-ions series, which allows for some sequencing of the protein. One great advantage of ISD is its fastness and easiness to be implemented since there is no need for a special treatment of the sample. The only requirement is the use of “ISD-favourable MALDI matrix” such as 2,5-dihydroxybenzoic acid or 1,5-diaminonaphtalene. 18 µm-thick equatorial sections of frozen porcine eye lenses were realized with a cryostat. 1,5-DAN matrix was either manually deposited or sprayed with an ImagePrep automated device (Bruker Daltonics). Data were acquired with an UltraFlex II MALDI-TOF/TOF mass spectrometer (BD) in positive reflector mode. For imaging experiments, the surface of the sample was divided into 100-µm-wide pixels and 500 shots were averaged on each. Based on calculated mass differences between consecutive ISD fragments peaks, tags of amino acids were established and submitted to a search in protein databases using a BLAST algorithm (search by sequence homology). Imaging experiments showed that the localization information may be very useful to associate fragments which exhibit close distributions, suggesting they are originating from the same protein. It is thus possible to arrange fragments in groups of probable origin and to extract the mass spectrum of a high-intensity pixel. This allows to work with a “purified” ISD mass spectrum where fragments of only one protein are present and potentially exhibiting a higher number of peaks, leading to a longer tag and to an easier identification. With this imaging strategy, we were able to identify (by homology) the Beta-Crystallins S and B2, the Gamma-Crystallin B, the Alpha-Crystallin A. [less ▲]

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See detailComparison of Spectral Colorimetric Measurements vs. Color Pictures in Dermatology
Blain, Pascal ULg; Michel, Fabrice; Moreau, Vincent et al

Poster (2010, April 13)

We studied scars and wounds depths and surfaces thanks to our interferometric fringes projector 3D scanner1, 2. Color information of a wound indicates its deterioration level. That’s why the visual color ... [more ▼]

We studied scars and wounds depths and surfaces thanks to our interferometric fringes projector 3D scanner1, 2. Color information of a wound indicates its deterioration level. That’s why the visual color restitution, as realistic as possible, is a highly important parameter. Firstly our acquired 3D pictures were color mapped with an image recorded by a RGB camera. The results were not efficient enough. In order to improve our technique and provide more precise information, we add a spectral characterization to the set-up. Before adding the spectral information and a realistic color mapping to the 3D measurements, we evaluate the performances of colorimetric measurements. The tests have been made on mice with scars on their back. [less ▲]

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See detailForecasting epidemic outbreaks ofwheat leaf blotch based on meteorological parameters
Junk, Jürgen; El Jarroudi, Moussa ULg; Pogoda, Frederik et al

Poster (2010, April 12)

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See detailMonitoring of surface interactions as a tool for nanoparticle design
Frost, R; Grandfils, Christian ULg; Kasemo, B et al

Poster (2010, April 07)

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See detailMICROPOLLUTANTS ISSUES: A MODELLING STUDY OF HEAVY METALS WITHIN TWO FRENCH BASINS AND A TEST APPLICATION TO COCAINE IN BELGIUM
Deliège, Jean-François ULg; Everbecq, Etienne ULg; Grard, Aline ULg et al

Poster (2010, April)

European policies (European Framework Directive: Water 2000/60/CE, Directive 76/464/CE) impose to reduce the releases of about a hundred substances in surface water. In the last years, the AQUAPOLE has ... [more ▼]

European policies (European Framework Directive: Water 2000/60/CE, Directive 76/464/CE) impose to reduce the releases of about a hundred substances in surface water. In the last years, the AQUAPOLE has been involved in two studies related to this matter. First, on the request and with the financial support of both the French Ministry of Environment and ONEMA (“Office National de l’Eau et des Milieux Aquatiques”), INERIS (“Institut National de l’Environnement Industriel et des Risques”) drew up the guidelines of a methodology allowing fixing the local Limit Values to Emission so as to abide by the quality standards on the whole watershed. Within this context, INERIS wishes to test the use of pollutant transfer models on pilot sub-basins. The PEGASE model has been used to simulate micropollutants on two concrete use cases (1): (iii) in the Meuse sub-basin, managed by the French Water Agency Rhine-Meuse, for simulations concerning cadmium and zinc; (iv) in the Adour sub-basin, managed by the French Water Agency Adour-Garonne, for simulations applied to cadmium and copper. The choice of each substances and sub-basins was made by mutual agreement between INERIS, the concerned Water Agencies, and the AQUAPOLE. A major selection criterion for the substances and the sub-basins was the availability of data (sources and in situ measurements). For the second study, the PEGASE model has been adapted to describe the cocaine’s behaviour (using a stable metabolite of the cocaine in the environment: the benzoylecgonine (BZE)) in waste water, waste water treatment plants (WWTP) and surface water (2). The cocaine is newly described in the model as an additional micropollutant (PEGASE already treats numerous heavy metals), thanks to the implementation of new state variable equations and their specific parameterizations. Simulations of BZE have been done in the Walloon and Flemish regions, where many measurements from the COWAT project (3) were available. The first results are showing good agreement between calculated and measured values. The ability of the model to simulate the fate of studied micropollutants (cadmium, zinc, copper, and the cocaine derivatives) in surface waters should be enhanced and extended to other substances and basins. Moreover, additional data still have to be collected and measured. [less ▲]

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See detailEvaluation de la distribution d’abondance des plantes vasculaires au Parc National de la Ruvubu, Burundi.
Masharabu, T; Noret, N; Lejoly, J et al

Poster (2010, April)

Detailed reference viewed: 26 (2 ULg)
See detailQuantitative determination of cannabinoids in serum by UHPLC/MS-MS
Dubois, Nathalie ULg; Paccou, A.; DE BACKER, Benjamin ULg et al

Poster (2010, April)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques et al

Poster (2010, April)

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See detailTRANSNATIONAL MODELING OF THE SURFACE WATER QUALITY OF THE INTERNATIONAL RIVER BASIN DISTRICT MEUSE WITH PEGASE
Deliège, Jean-François ULg; Everbecq, Etienne ULg; Grard, Aline ULg

Poster (2010, April)

The PEGASE Model has been applied at the entire International River Basin District of the Meuse. The poster presents different sub-basin applications on (i) the Chiers (Luxembourg + Belgium Walloon Region ... [more ▼]

The PEGASE Model has been applied at the entire International River Basin District of the Meuse. The poster presents different sub-basin applications on (i) the Chiers (Luxembourg + Belgium Walloon Region and French Rhin-Meuse Basin, 2000-2006) for the reference years 2000 and 2002 and prospective scenario 2015, (ii) International simulation performed (during 2005-2006) upstream (up to the Belgian border) for the reference year 2002, (iii) Current coordination between BE-Flemish Region and NL for downstream simulations of historical situation and (iv) prospective scenarios : International databases for a single reference year on the whole Meuse District [less ▲]

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See detailAtopy controlling loci in Czech and Russian populations
Gusareva, Elena ULg; Badalová, Jana; Havelková, Helena et al

Poster (2010, April)

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See detailHydrological modelling of the EU Nitrates Directive Actions Programme: new developments in the Walloon Region (Belgium)
Sohier, Catherine ULg; Dautrebande, Sylvia; Degre, Aurore ULg

Poster (2010, April)

Wallonia (Southern Region of Belgium) implemented the Nitrates Directive through a first actions plan in 2002 followed by a second action plan in 2007. It designated vulnerable zones and introduced ... [more ▼]

Wallonia (Southern Region of Belgium) implemented the Nitrates Directive through a first actions plan in 2002 followed by a second action plan in 2007. It designated vulnerable zones and introduced various mandatory practices in order to reduce the nitrate contamination risk. At the same time, the government decided to fund non mandatory practices focused on agro-environment. Some of these (like buffer strips) should also be useful in nutrient mitigation. In order to assess the global effectiveness of all the mitigation practices, we have been developing a hydrological model spatially distributed using a 1km² grid cell on the whole Region (16 900 km²). The EPICgrid model represents the root zone and the vadose zone. In Wallonia, groundwater tables are more than 30 m deep in 8% of the territory. It is therefore of major importance to fill the gap between the root zone and the groundwater bodies. It allows us to assess the nitrate transfer time and forecast the mitigation measures’ effect in time, space and amplitude. On the ground, runoff and sediment yield are modelled at the small watershed scale in order to assess buffer strips’ effect on sediment deposition and its consequences on N and P mitigation. The poster will explain the more recent results that consist in forecasting action plans’ effect until 2015 (on both surface water bodies and groundwater bodies) and modelling of the current buffer strips’ effect. [less ▲]

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See detailLipase-assisted synthesis of potential bio-based surfactants starting from lignocellulosic carbohydrates
Richard, Gaetan ULg; Nott, Katherine ULg; Paquot, Michel ULg et al

Poster (2010, April)

Surfactants constitute an important class of compounds with a lot of applications, especially in the food and beverage industries (emulsion forming and stabilization, antiadhesive and antimicrobial ... [more ▼]

Surfactants constitute an important class of compounds with a lot of applications, especially in the food and beverage industries (emulsion forming and stabilization, antiadhesive and antimicrobial activities)1. With the prospect of synthesising new active compounds, white biotechnology offers efficient tools. Indeed, the use of enzymes as biocatalysts provides an interesting synthetic route in comparison to the chemical way that often requires high reaction temperatures and suffers from a lack of specificity, resulting in complex mixtures. Among all the biocatalysts available, lipases represent a class of industrial interest 2,3. In parallel, the starting material is also an important parameter : due to the depletion of petroleum reserves, its ever increasing price and various environmental aspects, the use of renewable or biomass resources is inevitable. Within the frame of a biorefinery project, we focused on the lipase-assisted modification of uronic acids. These carbohydrates can be obtained from lignocellulosic material, and, in the presence of fatty alcohols, the Candida antarctica lipase B catalyses the synthesis of amphiphilic compounds. Owing to the initial conditions used and the addition of co-solvents, we optimized the synthesis of these bio-based potential surfactants, and obtained a panel of various structures depending to the acyl acceptor used. The poster will present the details of these syntheses. [less ▲]

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See detailThe influence of genetic selection on the milk fatty acid profile of spring calving dairy cows
Mc Parland, Sinead; Berry, Donagh; Coleman, J. et al

Poster (2010, April)

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See detailApproach of regionalization of low flow of the Walloon Region
Gailliez, Sébastien; Degre, Aurore ULg

Poster (2010, April)

The walloon part of the Meuse’s watershed represents 12283 km² and 17694 km of river. The anthropogenic pressure is important (population density is more or less 200 inhabitants/km²). In low flow period ... [more ▼]

The walloon part of the Meuse’s watershed represents 12283 km² and 17694 km of river. The anthropogenic pressure is important (population density is more or less 200 inhabitants/km²). In low flow period, water scarcity can touch both the water users (producer of drinking water and hydropower, tourism and pleasure activities and kayaking) and the river itself affecting the ‘good environmental state’ (context of Water Framework Directive 2000/60/CE). The operational management of rivers during low flow periods needs a deep knowledge of this drought phenomenon including an analysis of low flow severity and its occurrence probability. It also needs the computation of low flow discharge at any point of a river based on available hydrologic variables. The aim of this study is the low flows’ regionalization in the Walloon Region. First of all, the time series of flow data are filled in and validated. The quality is then controlled. The different tests are the determination of the minimum year requirement for a monitoring site, homogeneity tests, verification of presence or absence of summer alga and the proportion of extrapolation of the discharge rating curve Secondly, homogeneous regions will be defined and regression equations will be build. These equations will establish the relation between low flow and physical parameters (watershed area, pedology, slope), climate ones and/or ground water ones. The regression model will permit the computation of low flow discharge at any point of an ungauged river. [less ▲]

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See detailEvaluation of climate changes impact on the hydrological behaviour of the peaty mediums - Application to the Haute Lesse-Ourthe area (Belgium).
Sohier, Catherine ULg; Dautrebande, Sylvia; Degre, Aurore ULg

Poster (2010, April)

The peaty and wet mediums have a very important role to play in term of biodiversity conservation, hydrologic pattern regulation, fauna habitat and landscape value. In the Haute Lesse – Ourthe catchments ... [more ▼]

The peaty and wet mediums have a very important role to play in term of biodiversity conservation, hydrologic pattern regulation, fauna habitat and landscape value. In the Haute Lesse – Ourthe catchments (Saint-Hubert), numbers of these mediums were strongly modified by a drainage network. This drainage network was implemented to permit a forestry production focussed on the Spruce. It has severely disturbed the hydrological cycle. The study consists in modelling the impacts of restoration practices carried out notably within the framework of the LIFE European project on the hydrological behaviour of the peat bog. Then we modelled the effect of climate change. Physical properties and retention curve of peaty materials were characterized on the basis of undisturbed samples using the Richards’apparatus. Calculations were carried out with the hydrological model EPICgrid (GxABT). The model was initially validated on several reference catchments of the study zone. It was then applied at local scale for various peaty mediums (peat type, drainage characteristics, vegetation type) and for current and future climatic conditions. A regional simulation was finally carried out in order to evaluate the impacts of peat bogs restoration measures at catchment scale. Simulations highlighted the differences in physical and hydrous properties of the different peat types (“true” peat and “degraded” peat). Local and regional impacts of peat bogs restoration on their hydrological behaviour have been evaluated. Eventually, simulations allowed us to evaluate climate change’s impact on the hydrological behaviour of the peaty mediums. The model showed notably that future climatic conditions could generate severe droughts which can become critical in some situations. [less ▲]

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See detailA pendulum test as a tool to evaluate viscous friction parameters in the equine fetlock joint
Noble, Prisca ULg; Lumay, Geoffroy ULg; Coninx, Marc ULg et al

Poster (2010, April)

This study describes an equine fetlock joint pendulum test, and investigates the influence of post mortem time and intra-articular lipid solvent on the viscous frictional response. Fresh equine digits ... [more ▼]

This study describes an equine fetlock joint pendulum test, and investigates the influence of post mortem time and intra-articular lipid solvent on the viscous frictional response. Fresh equine digits (control group 1 (n = 6); lipid solvent group 2 (n = 6)) were mounted in a pendulum tribometer. Assuming that pendular joint damping could be modelled by a harmonic oscillator fluid damping (HOFD), damping time (t), viscous damping coefficient (c ) and friction coefficient (µ) were then followed for 5 hours under experimental conditions (400 N; 20 °C). In all experiments, pendular joint damping was found to follow an exponential decay function (R² = 0.99714), which confirms that joint damping is fluid. The evolution of t, c and µ was found to be significantly (P <0.05) different in the two groups. Indeed, t decreased and c and µ increased more rapidly and strongly in the group 2. It was concluded that pendular joint damping can be modelled by an HOFD model. The influence of post mortem time on results suggests that, ideally, joint mechanical properties should only be tested on fresh cadavers at the same post mortem time. Finally, the addition of lipid solvent was found to be responsible for upper viscous friction parameters and for a reduced damping time, which suggests that articular lubricating ability was compromised. This equine pendulum test could be used to test the efficacy of various bio-lubricant treatments. [less ▲]

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See detailSeaDataNet regional climatologies: an overview
Troupin, Charles ULg; Ouberdous, Mohamed ULg; Barth, Alexander ULg et al

Poster (2010, March 29)

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See detailGODIVA: a 4-dimension implementation of DIVA
Troupin, Charles ULg; Ouberdous, Mohamed ULg; Beckers, Jean-Marie ULg

Poster (2010, March 29)

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See detailVariation in external morphology of resident bottlenose dolphins (Tursiops truncatus) in Bahia San Antonio, Patagonia, Argentina.
Vermeulen, Els ULg; Cammareri, Alejandro

Poster (2010, March 22)

Two geographic variations of bottlenose dolphins were described in Argentina (Bastida & Rodriguez, 2003); bottlenose dolphins characterized by their triangular dorsal fin shape (coast of the province of ... [more ▼]

Two geographic variations of bottlenose dolphins were described in Argentina (Bastida & Rodriguez, 2003); bottlenose dolphins characterized by their triangular dorsal fin shape (coast of the province of Buenos Aires), and bottlenose dolphins characterized by their falcate dorsal fin shape (coast of the province of Chubut). It was stated that `their clear difference would indicate that both geographic forms are isolated¿ (Bastida & Rodriguez, 2003 p.137). A photo-identification study carried out in Bahía San Antonio (BSA), North Patagonia Argentina, showed a similar variation in external morphology among year-round resident bottlenose dolphins. Out of the 15 bottlenose dolphins considered year-round resident in the bay (Vermeulen & Cammareri, 2009), three are clearly distinguishable by a more falcate dorsal fin, a darker coloration and a notably shorter beak. These individuals, with one associated calf, were first identified in September 2008 and could be re-identified in the study area up to 13 days over all the different seasons. On all occasions, they were re-identified in close association with each other and on 10 occasions in close association with triangular dorsal fin shaped bottlenose dolphins. Behavioural observations made during these associations indicated that these mixed dolphin groups were 18% of their time feeding, 18% socialising, 17% slowly travelling and resting, 16% travelling in medium and fast speed and 7% milling (n=380 min). Although both forms show variations in external morphology, the extent to which this phenotypic variation is genetically correlated remains unknown. A clear insight on the differentiation between these regional forms might have important conservation implications for this species in Argentina. [less ▲]

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See detailImpact of the crystallisation pathway of inulin on its mono-hydrate to hemi-hydrate thermal transition
Ronkart, Sébastien; Deroanne, Claude; Paquot, Michel ULg et al

Poster (2010, March 22)

Inulin plays a major role in the food industry as dietary fibre, bulking agent and fat or sugar substitute. Thermal properties of two inulins obtained from different crystallisation pathways were ... [more ▼]

Inulin plays a major role in the food industry as dietary fibre, bulking agent and fat or sugar substitute. Thermal properties of two inulins obtained from different crystallisation pathways were investigated. The first one, was obtained by fractional precipitation of a saturated inulin solution (40/120). The second one, came from a solid amorphous inulin (95/120). These two inulins were spray dried with an inlet air temperature of 120°C. Then, these powders were placed into controlled atmosphere conditions at 0 % HR during one week and at 94% during the same time. Finally the two inulins were called 40/120/94% and 95/120/94% and their thermal properties were investigated by Wide angle X-ray scattering (WAXS), differential scanning calorimetry (DSC), temperature resolved wide angle X-ray scattering (TRWAXS) and thermogravimetry (TGA).Although WAXS did not show any difference between the two inulin, thermal analysis revealed the existence of a hemi-hydratate inulin (40/120/94%). This fact has an implication on the processing of the powder at an industrial level. Indeed, 40/120/94% has shown an agglomeration whereas 95/120/94% was a continuous mass. In other words, polymorphism of inulin crystals induces technofunctionality changes of the powder (hygroscopy, solubility, etc.) which could have an impact during processing or formulation of the powder. [less ▲]

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See detailNanocoatings of inorganic surfaces by molecular biomimetic
Vreuls, Christelle ULg; Genin, Alexis ULg; Zocchi, Germaine ULg et al

Poster (2010, March 22)

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See detailPostoperative sialadenitis with chronic brachial plexopathy
Reuter, Gilles; Racaru, Tudor ULg; Nguyen khac, Minh-Tuan et al

Poster (2010, March 20)

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See detailRelease of cardiac biomarkers after an intense physical exercise: preliminary results
Le Goff, Caroline ULg; Laurent, Terry; Chapelle, Jean-Paul ULg et al

Poster (2010, March 20)

Background: Over the past 2 decades, there has been a large interest in cardiac markers elevations, which are often seen following endurance sport events. These elevations were transient, with levels ... [more ▼]

Background: Over the past 2 decades, there has been a large interest in cardiac markers elevations, which are often seen following endurance sport events. These elevations were transient, with levels decreasing to pre-event concentrations within 24-48 hours. This might be explained by the relatively short half-life of studied markers, or water imbalance during and after the event. Therefore, the present preliminary study aimed to examine the increase in N-terminal pro-brain natriuretic peptide (NT-proBNP), highly sensitivity cardiac troponin T (hsTnT) and I (TnI II), myoglobin, creatine kinase muscle – brain (CK-MB), myeloperoxydase (MPO) and Highly sensitive C-reactive protein (hs-CRP) elevations after prolonged strenuous exercise . Materials and methods: Blood samples (EDTA plasma and heparinised plasma) were drawn at baseline, after 45, 90, 105, 165, 225, 285, 345, 690 and 1440 minutes in two healthy persons (29 year, trained 6 hours per week; 23 year, untrained). Each subjects runs at the maximal possibility during 2 hours. Results: For the untrained person, level of NT-proBNP exceeded the upper reference limits 12 hours after exercise but increased in all times. HsTnT and TnI II levels were upper the reference limit respectively 45 minutes and directly after exercise and increased up to 4 hours after exercise. We reported a decrease of these concentrations above the reference limits after 24 hours. Myoglobin increased after 45 minutes until 5 hours after exercises. It decreased after the 5th hour to be normalized 24 hours after exercise. CK-MB increased directly after the exercise and was upper the reference limits 165 minutes after the exercise. Level of MPO was very high just after exercise and decreased quickly in the following hours to be just upper the limit references 24 hours after exercise. HsCRP levels increased after 105 minutes and continued to increase after 24 hours. For the trained subject, we noted the same profile of increase of cardiac markers levels stayed but in the range of reference. Conclusion: These cases are extremely interesting. Indeed, this observation suggested a physiological counter regulatory process rather a simple increase of myocardial damage related to the intensity of exercise. In fact, for this moment, we do not know if the release of cardiac markers is physiological or pathological thus it must be studied. This preliminary study on endurance training suggested that intensively is determinants of the rate and the magnitude of subsequent cardiac marker release. These results suggested that an adaptation mechanism could exist. Benefits and possible long-term negative aspects of prolonged exercise should be evaluated with a more important population of athletes. [less ▲]

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See detailGPS™ II and GPS™ III: comparison of obtained platelets concentrations
Kaux, Jean-François ULg; Le Goff, Caroline ULg; Renouf, Julien et al

Poster (2010, March 20)

Introduction: Recently, several researches, essentially in vitro, demonstrated the positive effects of platelets on healing process of different tissues: bones, muscles and tendons. The aim of this study ... [more ▼]

Introduction: Recently, several researches, essentially in vitro, demonstrated the positive effects of platelets on healing process of different tissues: bones, muscles and tendons. The aim of this study is to compare the obtained platelets concentration between the new GPS™ III and GPS™ II. Methods: Two blood samples of 52 mL were taken in 5 volunteers and transferred respectively in both GPS™ II and GPS™ III. These devices were centrifuged at 3200 RPM during 15 min. The platelet-rich plasma (PRP) was thus collected and transferred in 6 mL test tubes. Cells count was done using an analyser ABX Micros 60. Results and conclusion: Platelets concentrations were more important from 6.2 up to 9.2 times with GPS™ II and from 7.3 up to 8.3 times with GPS™ III compared to blood samples. Efficiency of the collected platelets was around 92% for GPS™ II and 96% for GPS™ III. Both techniques made it possible to collect platelets but, unfortunately, also a lot of red and white blood cells. None of these parameters showed any significant difference (p>0.05). Conflicts of interests: The 10 devices GPS™ II and GPS™ III were provided gracefully by the firm Biomet Biologics TTC. [less ▲]

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See detailComparison between platelet-rich plasma (PRP) and vascular endothelial growth factor-111 (VEGF-111) as a therapeutic tool in tendon healing process
Kaux, Jean-François ULg; Drion, Pierre ULg; Libertiaux, Vincent ULg et al

Poster (2010, March 20)

Introduction In spite of the availability of various treatments for tendinopathy, this pathology often becomes chronic. For this reason, it is of interest to develop new treatments. Among them, the ... [more ▼]

Introduction In spite of the availability of various treatments for tendinopathy, this pathology often becomes chronic. For this reason, it is of interest to develop new treatments. Among them, the injection of platelet-rich plasma (PRP) seems to be a promising one. Indeed, several animal models have demonstrated that injection of blood platelets can initiate and stimulate tendon and ligament repair by releasing growth factors (GF) locally. Among all the GF released by activated platelets, the vascular endothelial growth factor-A (VEGF-A) is known to induce positive effects on vascular function and angiogenesis, and could be implicated in the healing process of tendons. Recently, a novel VEGF-A isoform was identified, the VEGF-111, a biologically active and proteolysis-resistant VEGF-A isoform, also known to present beneficial effects on ischemic diseases. This prompted us to evaluate whether VEFF-111 would have a therapeutic interest within the framework of the tendon pathology. Aim of the study: We hypothesized that the healing of ruptured Achilles tendons, which is the last stage of the Blazina’s classification, could be improved by injection of VEGF-111 that was compared to the potential effect of PRP injections using a rat model. Methods: A 5mm defect was surgically induced in rat Achilles tendon after resection of plantaris tendon. Rats were divided into 3 groups: A: control (no injection), B: PRP treatment and C: VEGF-111 treatment. Rats received a local injection of PRP (50µL) or VEGF-111 (100ng) in situ after the surgery and were placed in their cage without immobilization. After 5, 15 and 30 days, the rats were euthanized in each group. The traumatized Achilles tendon of each rat was removed and dissected during the healing process. Immediately after sampling, tendons were submitted to a biomechanical tensile test up to rupture, using a “Cryo-jaw”. Results: Our results show that developed force necessary to induce tendon rupture during biomechanical tensile test was more important for tendons which had received an injection of PRP or VEGF-111. Moreover, the tensile force necessary to break tendons is higher with PRP than with VEGF-111. These results were already noticed from day 5 onwards. Conclusion: This experimentation has shown that both PRP and VEGF-111 injections stimulated tendon healing process as suggested by the increased force needed to break tendons during its healing process. Furthermore, this acceleration of the cicatrisation process was more significant with PRP than with VEGF-111. This could be explained by the release from platelets of a “cocktail” of growth factors acting in synergy on the healing process. Acknowledgement This experimentation was partially financed by “Standard de Liège 2007” and “Lejeune-Lechien 2008” grants. [less ▲]

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See detailMagnetic hybrid based on iron oxide nanoparticles and thermoresponsive block copolymer for biomedical applications
Sibret, Pierre ULg; Aqil, Abdelhafid; Zhao, J. et al

Poster (2010, March 18)

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See detailTargeting nanoparticles to M cells with non-peptidic ligands for oral vaccination
Freichels, Hélène ULg; Fievez, Virginie; Plapied, Laurence et al

Poster (2010, March 18)

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See detailZebrafish as model in toxicology/pharmacology.
Voncken, Audrey ULg; Piot, Amandine ULg; Stern, Olivier ULg et al

Poster (2010, March 17)

Detailed reference viewed: 87 (35 ULg)