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See detailBone regeneration using porous titanium particles vs. bovine hydroxyapatite: a sinus lift study in rabbits
LAMBERT, France ULg; LECLOUX, Geoffrey ULg; Léonard, Angélique ULg et al

Poster (2011)

Background: The use of porous titanium particles as space filling biomaterials for intraoral bone regeneration might be of interest because of both the mechanical stability and nonresorbable properties ... [more ▼]

Background: The use of porous titanium particles as space filling biomaterials for intraoral bone regeneration might be of interest because of both the mechanical stability and nonresorbable properties. Titanium particles were recently described for bone regeneration in sinus lifts and in the treatment of periimplantitis. Nevertheless, tissue integration and 3D bone regeneration with titanium particles were poorly explored in these previous reports. Aim: The first objective of this study was to qualitatively and quantitatively assess the bone formation process, particularly the long-term behavior and 3D volume stability of subsinusal bone regeneration, using titanium or bovine hydroxyapatite granules, in a rabbit model. The second objective was to evaluate the effect of the hydration of the BHA particles with a therapeutic concentration of doxycycline solution on the osteogenesis and biomaterial resorption. Methods: Rabbits underwent a double sinus lift procedure using one of three materials: grade 1 porous titanium particles (Ti), bovine hydroxyapatite (BHA) or chemically modified bovine hydroxyapatite (BHATTC). Animals were sacrificed after 1 week, 5 weeks or 6 months. Samples were analyzed using mCT and nondecalcified histology. Results: The materials used in each of the three groups allowed an optimal bone formation; bone quantities and densities were not statistically different between the three groups. At 6 months, more stable 3D volume stability was found with TI and BHATTC (P¼0.0033). At 5 weeks and 6 months, bone to material contact (BMC) corroborating osteoconduction was significantly higher with BHA and BHATTC than with Ti (P < 0.0001). Conclusions and clinical implications: Even though the studied biomaterials displayed different architectures, they are relevant candidates for sinus lift bone augmentation before dental implants because they allow adequate 3D stability and osteogenesis. However, to recommend the clinical use of Ti, both an observation on the drilling effects of Ti particles and clinical trials are needed. [less ▲]

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See detailProduction, extraction et concentration en continu de biosurfactants produit par B. subtilis en bioréacteur à membrane
Coutte, F.; Lecouturier, D.; Leclere, V. et al

Poster (2011)

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See detailDescription of the bone-remodeling pattern after socket preservation procedures in human: a methodological study
LAMBERT, France ULg; VINCENT, Kim ULg; VANHOUTTE, Vanessa ULg et al

Poster (2011)

Background: The bone remodeling after tooth extraction can result in the loss of as much as 50% of the buccal wall and a global crestal reduction in width of 3.87mm according to a recent systematic review ... [more ▼]

Background: The bone remodeling after tooth extraction can result in the loss of as much as 50% of the buccal wall and a global crestal reduction in width of 3.87mm according to a recent systematic review. Multiple surgical protocols using biomaterials are proposed in order to limit the typical postextraction bone resorption. However, because of the heterogeneity of the studies and, more specifically, of the assessment methods, it is difficult to assert the superiority of one technique over another. Aim: The objective of this study was to develop a new radiographic method to assess alveolar bone remodeling after socket preservation procedures. Methods: Sixteen extraction sites (in 14 patients) localized in the upper anterior maxilla were treated with bovine hydroxyapatite (0.25–1mm particles) and a saddled connective tissue graft. A radiographic 3-dimensional assessment of the hard tissues was performed at baseline and at 3 months after the procedure. Standardized horizontal measurements were taken at three corono apical levels ( 2, 5, and 9mm) and at three mesio-diatal level (Mesial, Center, and Distal) in the buccal and palatal aspects. Vertical measurements were also recorded in nine regions over the top of the alveolar crest. Results: Extraction socket-preservation technique assessed in the present study significantly reduced horizontal bone remodeling. The horizontal dimension of the crest decreased by 1.6mm (20%) in the cervical regions ( 2mm level), experienced a moderate decrease of 1mm (12%) at the 5mm level, and experienced a very low decrease of 0.5mm(6%) in the apical ( 8mm) level. The losses were always significantly higher in the buccal than in the palatal aspect. Buccally, the maximal bone remodeling at the cervical level remained below 1mm. Vertical bone resorption was homogeneous and was < 1mm in the nine measured regions. Conclusions and clinical implications: The radiographic measuring methodology proved to be accurate and reproducible. It can be applied in other clinical settings. Moreover, the surgical procedure evaluated in the present study, significantly limits the postextraction buccal bone remodeling compared with the data found in the literature for untreated extraction socket in the aesthetic area. However, a complete inhibition of the bone remodeling was not reached and the authors suggest a surgical technique using a ‘‘saddled’’ connective tissue graft to thicken buccal soft tissue biotype and consequently compensate for cervical bone loss. [less ▲]

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See detailBioinformatics tools to decrypt pyoverdine biosynthesis in Pseudomonas sp.
Vanvlassenbroeck, Aurélien; Leclère, Valérie; Pupin, Maude et al

Poster (2011)

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See detailHighly efficient organic/inorganic titania xerogels for photocatalysis applications
Bodson, Céline ULg; Bied, Catherine; Páez Martínez, Carlos ULg et al

Poster (2011)

TiO2 heterogeneous photocatalysis is an attractive technique for the complete destruction of undesirable contaminants either in aqueous or gaseous phase by using solar or artificial light illumination ... [more ▼]

TiO2 heterogeneous photocatalysis is an attractive technique for the complete destruction of undesirable contaminants either in aqueous or gaseous phase by using solar or artificial light illumination. The main drawback of TiO2 is that it can be activated only by UV light because of its large band gap (3.20 eV for anatase). Thus, it would be interesting to sensitize TiO2 to the whole visible region by doping with non-metal atoms such as phosphorus. Furthermore, it was found that the P-doped species could significantly increase the specific surface area of the materials, which consequently exhibit a higher content of surface hydroxyl groups. Upon band gap excitation of TiO2, the photoinduced electrons and positively charged holes can reduce and oxidize the species adsorbed on the TiO2 particles. The high degree of recombination between photogenerated electrons and holes is a major rate-limiting factor controlling the photocatalytic efficiency. Attempts to increase the titania efficiency have been made by doping P-doped TiO2 with metals, such as Ag. In the present study, a sol-gel method is developed to synthesize P-doped TiO2 and P/Ag-codoped xerogels by a cogelation method [1] based on the hydrolysis and the condensation of Ti(OC3H7)4 in the presence of a phosphoryled compound able to complex silver: NH2-(CH2)2-NH-(CH2)2-P(O)-(OC2H5)2, EDAP), in various alcohols. These xerogels are dried at 150°C under vacuum for 24 h, and calcined under air for 5 h at 350°C, 450°C, 550°C and 650°C. The resulting materials were characterized by ICP-AES, TG-DSC, TEM, XRD, nitrogen adsorption-desorption isotherms, FT-IR and diffuse reflectance measurements in the UV/Vis region. To measure the photoactivity of these new hybrid organic/inorganic titania xerogels, all these samples were tested for the degradation of p-nitrophenol under visible light. The titanium, phosphorus and silver contents in xerogels were confirmed by ICP-AES. As expected, theoretical and actual Ti/P molar ratio and Ag contents in xerogels are similar and then after washing with THF for 48 h. These results allow to conclude that EDAP and Ag are well incorporated within the TiO2 matrix: EDAP is anchored in the titania matrix by complexation and cogelation with TTIP [2], whereas silver, first incorporated by complexation through the ethylenediamine fragment of EDAP, is present in the xerogels in the form of nanoparticles. It was found that the phosphor-doped species could significantly increase the surface area of the materials. [2] It is noteworthy that the porosity of these materials is not affected by the addition of silver acetate during the synthesis, a very slight decrease of the specific surface area being observed in all cases. The organic/inorganic hybrid titania materials display very high photocatalytic efficiencies in the degradation of p-nitrophenol compared to pure titania. Deeper investigations concerning the structure of these materials should enable a better understanding of the photocatalytic mechanisms involved with these hybrids and will soon be reported. 1. S. Lambert, K. Y. Tran, G. Arrachart, F. Noville, C. Henrist, C. Bied, J. J. E. Moreau, M. Wong Chi Man, B. Heinrichs, Micropor. Mesopor. Mater. 2008, 115, 609. 2. C.J.Bodson1, S.D. Lambert1, C. Alié1, X. Cattoën2, J-P. Pirard1, C. Bied2, M.Wong Chi Man2, B. Heinrichs1 ,Micropor. Mesopor. Mater. 2010, 134,157 [less ▲]

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See detailPostglacial pioneer settlement in the Sarvinki area, Eastern Finland : environmental and economical setting
Pesonen, Petro; Hertell, Esa; Mannermaa, Kristiina et al

Poster (2011)

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See detailSynthesis of amonabactins by Aeromonas hydrophila : implication of NRPS of a unique iterate-alternative type
Leclère, Valérie; Subashkumar, Rathinasamy; Wathelet, Bernard ULg et al

Poster (2011)

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See detailPilot Trials of STAR Target to Range Glycemic Control
Penning, Sophie ULg; Le Compte, Aaron; Massion, Paul et al

Poster (2011)

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See detailSynthesis of amonabactins by Aeromonas hydrophila : implication of NRPS of a unique iterative-alternative type
Leclère, Valérie; Rathisamy, Subashkumar; Wathelet, Bernard ULg et al

Poster (2011)

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See detailEffect of Chronic confinement stress on growth, survival, blood cortisol and glucose of perch (Perca fluviatilis)
Rougeot, Carole ULg; Tomson, Thomas; Vandecan, Michaël et al

Poster (2011)

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See detailThe promotive impact of high temperature on flowering in root chicory (Cichorium intybus L.)
Mathieu, Anne-Sophie; Vandoorne, Bertrand; Quinet, Muriel et al

Poster (2011)

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See detailDETERMINATION OF TRIFLURALIN RESIDUES IN CULTURED CATFISH AND WATER.
Nguyen Quoc, Thinh; Tran Minh, Phu; Do Thi Thanh, Huong et al

Poster (2011)

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See detailControl of Polymyxa betae by Bacillus amyloliquefaciens lipopeptides
Desoignies, N.; Schramme, F.; Ongena, Marc ULg et al

Poster (2011)

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See detailUne thérapie destinée aux patients schizophrènes - Self-Awareness Therapy (SAT): Résultats préliminaires
Boulanger, Marie ULg; Pauly, Marc; Nachtergael, Hilde et al

Poster (2011)

Objective: Autonoetic awareness enables to mentally relive personal events in memory to be aware of one’s own experiences across subjective time and so to have a feeling of individuality and self ... [more ▼]

Objective: Autonoetic awareness enables to mentally relive personal events in memory to be aware of one’s own experiences across subjective time and so to have a feeling of individuality and self-direction. It is intimately associated with our awareness of ourselves as persons with a past and a future. Autonoetic awareness thereof contributes to construction of our identity by the recovery of past events, particularly specific events, and the imagination of future events. According to Danion’s theoretical model (2001), autonoetic awareness impairment is at the heart of schizophrenia. This deficit should depend on defective associations between separate aspects of the events during the recall and therefore, it is associated with a reduction in accessing specific information in autobiographical memory (AM). This impairment should explain identity perturbation, cognitive deficits, negative and positive symptomatology present in schizophrenia. Supporting this model, the present study evaluates the effect of a cognitive and behavioural intervention called Self-Awareness Therapy (SAT) on autonoetic awareness, AM and the capacity to imagine oneself in the future, in schizophrenia patients. Method: Fifteen patients with schizophrenia participated to SAT and, 8 patients took part in the Integrative Program Therapy (IPT) of Brenner. Before and after therapy, patients were asked to complete questionnaires to evaluate the autonoetic awareness, AM, projection into the future as well as affective and symptomatology scales. Results: For SAT, the participants reported more specific past and future events after than before the remediation therapy. Furthermore, patients reported significantly less depressive and anxiety symptoms as well as less negative symptoms after compared before the SAT. For IPT, no significant differences emerged. Discussion: These results are in line with previous study (Blairy et al., 2008) and support the notion that, in schizophrenia the retrieving of past specifics memories and projection into the future can be improved. La conscience autonoétique (CA) nous permet de revivre mentalement des évènements personnellement vécus, d’en prendre conscience à travers un temps subjectif et de ressentir un sentiment d’individualité. Elle contribue à la construction de notre identité via la récupération d’évènements passés, particulièrement les évènements spécifiques, et l’imagination d’évènements futurs. Selon le modèle de Danion (2001), une perturbation au niveau de la CA serait au cœur de la schizophrénie. Celle-ci résulterait d’un relâchement des associations entre les différents éléments d’un évènement lors du rappel et dés lors, serait associée à une réduction de l’accès aux informations spécifiques en mémoire autobiographique. Cette perturbation pourrait expliquer les troubles de l’identité, les déficits cognitifs ainsi que la symptomatologie présents dans la schizophrénie. Afin de remédier à ces déficits, nous avons élaboré une intervention cognitive et comportementale, destinée à des patients schizophrènes, qui s’appuie sur ce modèle. Cette étude présente les résultats préliminaires de la SAT en la comparant au 1er module du programme IPT. [less ▲]

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See detailLymphangiogenesis and extracellular matrix remodeling
Erpicum, Charlotte ULg; Detry, Benoît ULg; Paupert, Jenny ULg et al

Poster (2011)

Lymphangiogenesis, the formation of new lymphatic vessels from preexisting ones, is an important biological process associated with diverse pathologies, such as metastatic dissemination and graft ... [more ▼]

Lymphangiogenesis, the formation of new lymphatic vessels from preexisting ones, is an important biological process associated with diverse pathologies, such as metastatic dissemination and graft rejection. Our laboratory has previously identified MMP2 as a key regulator of lymphangiogenesis in vitro and in vivo. However, the exact function of MMP2 in this process is yet unknown. The present work aims at elucidating the mechanisms of MMP2 action during lymphangiogenesis. MMP2 could either act as a growth factor activator or as a regulator of matrix remodeling. To address this question, we studied the effect of MMP2 on lymphangiogenesis in an novel in vitro model of sprouting cells from small aggregates (spheroids) seeded in a collagen gel. In this model, quantification of the lymphangiogenic response is performed through computerized methods allowing the measurement of the distance of migration, but also the evaluation of how the cell are migrating. We evaluated the impact of MMP2 blockage through the use of physiological (TIMP2) or chemical inhibitors or by downregulating its expression with specific siRNA. The importance of extracellular matrix composition is evaluated by embedding these spheroids into different matrices (matrigel versus collagen; pepsinized collagen versus native collagen; different collagen concentrations). Our results reveal a modification of cell migration through collagen gel after MMP2 inhibition. The utilization of DQ collagen and microscopy refractance confirmed the importance of MMP2 collagenoyitic activity for lymphangiogenesis. [less ▲]

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See detailAn investigation of the relationships between foot conformation and distal interphalangeal joint effusion in young warmblood horses at the beginning of their training
Cubeddu, Francesca; Bastianacci, Valentina; Verwilghen, Denis et al

Poster (2011)

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See detailPeriodic bursts of non-Io DAM and its relationship to Jovian aurora phenomena
Rucker, H.; Panchenko, M.; Grodent, Denis ULg et al

Poster (2011)

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See detailAntibiotic Producing Bacteria : Researches and Perspectives
Tenconi, Elodie ULg

Poster (2011)

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See detailUsing putative anti-auxins to improve somatic embryo maturation of Abies nordmanniana
Tahiri, Abdelghani ULg; Misson, Jean-Pierre; Kevers, Claire et al

Poster (2011)

In Belgium, the culture of Nordmann fir (Abies nordmanniana) is mainly located in Wallonia region. The planted area is estimated in average at 5000 ha  that annualy produce some 4 million Christmas trees ... [more ▼]

In Belgium, the culture of Nordmann fir (Abies nordmanniana) is mainly located in Wallonia region. The planted area is estimated in average at 5000 ha  that annualy produce some 4 million Christmas trees destined in priority to the export market. In vitro propagation by somatic embryogenesis is considered as an alternative method to seedlings for producing homogeneous and orthotropic trees on a large scale. Currently, the performances of the somatic embryo (SE) maturation is still insufficient to consider any commercial application of the technology. In this way , the effect of three substances  (2,3,5-triiodobenzoïque acid  as TIBA, 2 - (p-chlorophenoxy)-2-methylpropionic acids as PCIB, p-hydroxbenzoïque as PHBA)  reported as ''anti-auxins'' have been tested with three embryogenic lines on the formation and maturation of normal somatic embryos. [less ▲]

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See detailThiamine triphosphate synthesis in Escherichia coli requires FoF1-ATPase
Gigliobianco, Tiziana ULg

Poster (2011)

Exponentially growing E. coli cells contain high amounts of thiamine diphosphate (ThDP), a well-known cofactor, but only ~20% is bound to apoenzymes. We showed that the abundant free ThDP is the precursor ... [more ▼]

Exponentially growing E. coli cells contain high amounts of thiamine diphosphate (ThDP), a well-known cofactor, but only ~20% is bound to apoenzymes. We showed that the abundant free ThDP is the precursor for two triphosphorylated derivatives, thiamine triphosphate (ThTP) and the newly discovered adenosine thiamine triphosphate (AThTP). Both compounds are produced under different conditions of stress. ThTP transiently accumulates in response to amino acid starvation, but, in contrast to AThTP, its synthesis requires the presence of a carbon source yielding pyruvate. Under such conditions, the amount of cellular ThTP may reach up to 20–60% of total thiamine, while it is generally a minor compound (0.1 to 1% of total thiamine). The biological role of ThTP remains uncertain until now, though in E. coli, it seems to be required for rapid adaptation to amino acid starvation. ThTP accumulation requires inorganic phosphate and is inhibited by anoxia and cyanide. It is also blocked by low concentrations of the protonophore CCCP and is inhibited by low concentrations of DCCD, an inhibitor of FoF1-ATP synthase. These results suggest that ThTP is synthesized by a mechanism similar to oxidative phosphorylation, i. e. the reaction ThDP + Pi  ThTP catalyzed by FoF1-ATP synthase and energized by respiration. This hypothesis is supported by the finding that E. coli with FoF1-ATP synthase mutated in various subunits are unable to synthesize ThTP in minimal medium in response to glucose exposure. Moreover, a strain in which the atp operon was deleted is unable to synthesize ThTP, but transformation with a plasmid encoding the atp operon totally restores this capacity. We recently demonstrated a similar chemiosmotic mechanism for ThTP synthesis in rat brain mitochondria suggesting a conserved mechanism for ThTP synthesis between bacteria and mammals. [less ▲]

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See detailRetrieving semantic information from persons’ names.
Barsics, Catherine ULg

Poster (2011)

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See detailIsotopic Composition and sources of Organic Carbon Pools within the Tana River Basin, (Kenya)
Tamooh, F.; Van Den Meersche, K.; Borges, Alberto ULg et al

Poster (2011)

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See detailBiophysical studies of trigger factors adapted to extreme biological temperatures
Struvay, Caroline; Piette, Florence; Feller, Georges ULg

Poster (2011)

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See detailCellulase involvement in the cellulose biosynthesis of Pseudomonas stutzeri
Delsaute, Maud ULg; Berlemont, Renaud ULg; Paulus, Virginie et al

Poster (2011)

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See detailLe Magdalénien dans le Nord-Ouest de l'Europe
Miller, Rebecca ULg; Noiret, Pierre ULg

Poster (2011)

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See detailDistribution and composition of organic carbon in the Tana River Basin, (Kenya)
Tamooh, F.; van den Meersche, K.; Borges, Alberto ULg et al

Poster (2011)

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See detailEtude des interactions membranaires de dérivés de l’acide glucuronique par calorimétrie à titrage isotherme
Laurent, Pascal ULg; Razafindralambo, Hary ULg; Paquot, Michel ULg

Poster (2011)

Dans le cadre de la valorisation de la biomasse et des glucides provenant de l’hydrolyse de matières premières renouvelables, différents dérivés d’acides uroniques ont été synthétisés en vue d’évaluer ... [more ▼]

Dans le cadre de la valorisation de la biomasse et des glucides provenant de l’hydrolyse de matières premières renouvelables, différents dérivés d’acides uroniques ont été synthétisés en vue d’évaluer leurs propriétés en tant qu’agents tensioactifs biocompatibles et biodégradables. Une nouvelle stratégie de synthèse assistée par micro-ondes, efficace et respectueuse de l'environnement, a par ailleurs permis de synthétiser quantitativement différentes D-glucofuranosidurono-6,3-lactones monosubstituées hydrosolubles au départ d'acide D-glucuronique non protégé en utilisant un catalyseur acide supporté. Ces lactones appartiennent à la famille des glycosurfactants non-ioniques dont les applications couvrent un vaste domaine, aussi bien dans l’industrie pharmaceutique, cosmétique ou alimentaire. Une première approche en vue d’évaluer leurs activités biologiques consiste en l’étude des paramètres d’interactions moléculaires de ces lactones vis-à-vis d’une membrane biologique modèle (1-palmitoyl-2-oleyl-sn-glycerol-3-phosphatidylcholine) par calorimétrie à titrage isotherme (Isothermal Titration Calorimetry – ITC). [less ▲]

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See detailSedimentology and magnetic susceptibility on a continuous Lower Givetian to Lower Famennian fore-reef succession (Sauerland, Germany) : A new example which reinforce the use of MS for long-distance correlations (Germany, Belgium and Moravia).
Pas, Damien ULg; Da Silva, Anne-Christine ULg; Boulvain, Frédéric ULg et al

Poster (2011)

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick ... [more ▼]

This study focuses on the continuous Givetian-Frasnian section of the abandoned Burgberg quarry (Messinghausen Anticline, northern margin of the Rhenish Mountains). The exposed section (102 m thick) covers a well constraint stratigraphic interval starting at the base of the Givetian (Stritzke 1991;Aboussalam et al., 2003) and according to Stritzke (1991) and our new datations ending within the Lower Famennian. The Middle-Upper Devonian shelf-edge within the Rheinisches Schiefergebirge can be traced from the supposed position along the southern rim of the Dinant Syncline and the Eifel Synclines, northwards along a line connecting the southern margin of the Devonian reefal outcrops of Attendorn and Brilon (Krebs 1967, 1974). The depositional setting of the investigated section corresponds to complex slope and basinal environments where reworked material from the proximal Brilon platform (located to the north) and basin deposits coexist. Thus, this section allows to follow the evolution of the Givetian-Frasnian Brilon platform (e.g., Machel 1990; Stritze 1990, 1991) in a deeper setting. Petrographic analysis of more than 330 thin-sections leads to the identification of 7 microfacies which are integrated into a palaeoenvironmental model. Microfacies curve evolution shows two main trends. A shallowing upward trend ending within a typical proximal slope setting (dismantling of the platform) followed by a deepening upward trend which is characterized by several meter of pelagic mudstone within the upper part of the studied section. Magnetic susceptibility variations in sedimentary rocks, have commonly been interpreted as related to variations of detritic inputs through climatic or sea level changes (Crick et al., 1994). The magnetic susceptibility (MS) study of more than 330 samples from this long-time fore-reef carbonated succession is an opportunity to better constraint our sedimentological interpretations. To do so, we propose a comparison between general MS trends and some parameters such as microfacies and relative sea level fluctuations interpreted on the basis of the sedimentological study. The relatively long stratigraphic interval covered by the Burgberg section offers a good opportunity to compare our data with the time equivalent Devonian sections of the Ardennes (Belgium) and Moravian karst area (Czech Republic) (Boulvain et al., 2010). And thus to test the magnetic susceptibility tool for long-distance correlation between stratigraphically well constraint sections. [less ▲]

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See detailEvolutionary significance and trade-offs in life-history traits associated to ecological specialization and mating systems in the liverwort genus Radula Dumort.
Laenen, Benjamin ULg; Devos, Nicolas; Renner, Matt et al

Poster (2011)

Shifts in mating systems are amongst the most common and important transitions in plants and are correlated with a suite of life-history traits. The evolution of mating systems and their relationships to ... [more ▼]

Shifts in mating systems are amongst the most common and important transitions in plants and are correlated with a suite of life-history traits. The evolution of mating systems and their relationships to gametophyte size, sexual reproduction, formation of asexual diaspores, and ecological specialization, is examined here in the leafy liverwort genus Radula.More specifically, we attempt to answer the following questions:(1)What is the ancestral mating system in Radula? (2)Are shifts from one mating system to another directional or random? (3)How does the evolution of mating systems correlate with the evolution of other related life history traits and, in particular, the specialization to temporary habitats? (4)What are the contingence relationships and order of acquisitionof those traits? [less ▲]

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See detailNew Methodology to detect toxin-GPCR binding by MALDI-TOF Mass Spectrometry
Echterbille, Julien ULg; De Pauw, Edwin ULg; Gilles, Nicolas et al

Poster (2011)

Introduction More than 50 thousands of venomous species are currently indexed in the world. Each of their venoms is composed of 200 to 1000 different toxins which potentially exhibit a high selectivity ... [more ▼]

Introduction More than 50 thousands of venomous species are currently indexed in the world. Each of their venoms is composed of 200 to 1000 different toxins which potentially exhibit a high selectivity for membrane receptors such as ionic channels or G-protein coupled receptors (GPCRs). GPCRs constitute the larger family of receptors since around 800 different kinds of them are knows. GPCRs are the target of around 30% of the current pharmacopeia drugs. Notable examples include Novartis’s Zelnorm, Eli Lilly’s Zyprexa and Schering-Plough’s Clarinex used to treat constipation, schizophrenia and allergies, respectively. Finding new GPCRs ligands appears of prime interest to design new pharmacological tools and potentially discover the drugs of our future. Interestingly, several toxins from venoms have already been described to bind to this particular family of receptor, opening the way to the discovery of new peptide drugs from animal venoms1-2. This work presents a pioneering MALDI-TOF/TOF based strategy to fish new GPCRs ligands from complex mixtures such as venom fractions. Methods The proof of concept of this methodology was built by studying the binding of [Arg8]-vasopressin (AVP) on type 2-vasopressin receptor (V2). Experimentally, fragments of cellular membranes over-expressing V2 receptors were incubated with cone snail’s venom fraction (~30 peptide toxins) doped by a small amount of AVP. After 2 hours incubation, free and bound fractions were carefully purified with a combination of centrifugation and micro column purifications. Samples were finally analyzed with a Bruker Ultraflex II MALDI-TOF/TOF mass spectrometer and the resulting spectra were interpreted with FlexAnalysis (v3.0), BioTools (v3.2) and SequenceEditor (v3.2) bioinformatics’ softwares from Bruker Daltonics. Preliminary data After the incubation of cellular membranes overexpressing V2 GPCR with a complex mixture of peptides doped by AVP, we clearly detect that the only V2 ligand present in the fraction was the AVP. Our result demonstrates the possibility to identify a ligand of GPCRs from a complex peptide mixture, such as venom fractions. Contrary to radiobinding, this approach allows detecting the direct binding of the toxin and does not imply to know a ligand of the studied GPCR before starting the experiments. This opens the way to the deorphanization of receptors (180 orphans GPCRs over 800). Moreover, since the new ligand is detected by mass spectrometry, it is directly identified from the mixture, without additional purification. Its structural characterization can be directly performed by de novo sequencing experiments. The drawback of our approach is the very long (but crucial!) sample preparation as each sample requires 2 purification steps (for both free and bound fraction). The next step of our work will be the automation of the procedure to allow a high-throughput screening of venom fractions on different GPCRs and the discovery of new ligands. Novel aspect GPCR’s ligands discovery by MALDI-TOF/TOF based techniques: a new pharmacological tool. 1 Quinton, L. et al. Isolation and pharmacological characterization of AdTx1, a natural peptide displaying specific insurmountable antagonism of the a1A-adrenoceptor. British Journal of Pharmacology 159, 316-325 (2010). 2 Rouget, C. et al. Identification of a novel snake peptide toxin displaying high affinity and antagonist behaviour for the α2-adrenoceptors. British Journal of Pharmacology 161, 1361-1374, doi:10.1111/j.1476-5381.2010.00966.x (2010). [less ▲]

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See detailMacroH2A regulates the commitment of adult and embryonic stem cells to differentation
Creppe, Catherine ULg; Cantariño, N; Janich, P et al

Poster (2011)

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See detailSeed dispersal by western lowland gorillas (G. g. gorilla) in south east Cameroon
Petre, Charles-Albert ULg; Tagg, Nikki; Beudels-Jamar, Roseline et al

Poster (2011)

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See detailUnpredictable photocatalytic ability of H2-reduced rutile-TiO2 xerogel in the degradation of dye-pollutants under UV and visible light irradiation
Páez Martínez, Carlos ULg; Lambert, Stéphanie ULg; Poelman, Dirk et al

Poster (2011)

Photocatalytic degradation of organic and inorganic pollutants on the TiO2 semiconductor has been extensively studied as a way to solve environmental problems relating to wastewater and polluted air ... [more ▼]

Photocatalytic degradation of organic and inorganic pollutants on the TiO2 semiconductor has been extensively studied as a way to solve environmental problems relating to wastewater and polluted air. Anatase and rutile are the most commonly used crystalline structures of TiO2, with anatase showing a higher photocatalytic activity attributed to its higher specific surface area and its favourable band gap energy (Eg). However, its high band gap (Eg = 3.2 eV) implies the use of UV light (lambda ≤ 380 nm) to inject electrons into the conduction band (TiO2(e-CB)) and to leave holes in the valence band (TiO2(h+VB)). Although the low band gap energy of rutile-TiO2 (Eg = 3.02 eV) allows rutile to potentially absorb more solar energy than anatase, the anatase-to-rutile phase transition leads to the collapse of the TiO2 specific surface area, which may result in a decrease in the photocatalytic activity of rutile. Low specific surface area and therefore poor absorption properties lead to strong limitations in exploring the photo-efficiency of rutile. Nevertheless, rutile has been proved to be comparable to anatase in its photoelectrochemical properties when used in dye-sensitized solar cells. In the present study, a new process for the reduction of rutile-TiO2 xerogel under hydrogen flow was developed to enhance the photocatalytic activity of TiO2 materials synthesized by the sol-gel process. So a series of H2-reduced TiO2 xerogels of low specific surface area was prepared by hydrolysis and condensation of tetraisopropoxy titanium(IV) in 2-methoxyethanol. The gels were dried under vacuum, calcined in air at different temperatures (400°C, 500°C and 700°C) and finally reduced in H2 at 400 °C. The materials were characterized by X-ray diffraction, transmission electron microscopy (TEM), FT-IR spectroscopy and UV/Visible diffuse reflectance spectroscopy. The texture was determined by nitrogen adsorption-desorption measurements. The effects of the calcination/reduction treatments on the adsorption of methylene blue (MB) in aqueous solution and on the photocatalytic degradation of MB and crystal violet (CV) under UV and visible light irradiation were also evaluated. Results showed predictable modifications in the physico-chemical properties caused by the annealing of TiO2 xerogel at high calcination temperature (700 °C), such as a total anatase-to-rutile phase transition and a considerable loss of specific surface area from 260 to 2 m2 g-1. However, the higher degree of reduction exhibited by the rutile-TiO2 lattice led to unpredictable photocatalytic activity for the dye conversion under UV and visible light irradiation: the loss of specific surface area of the rutile-TiO2 sample was compensated by the increase in the affinity of this sample for the dye. Under UV light irradiation, the rutile-TiO2 xerogel obtained after a calcination at 700 °C showed a similar level of photoactivity as the one obtained with anatase-TiO2 xerogels obtained by calcination at 400 °C and 500 °C. Under visible light, unlike anatase-TiO2 xerogels, the rutile-TiO2 xerogel showed a higher dye photoconversion rate per external surface area (40 times higher) than the commercial TiO2 Degussa P25. [less ▲]

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See detailTransient reduction of placental angiogenesis in PAI-1 deficient mice
LABIED, Soraya ULg; Blacher, Silvia ULg; Carmeliet, P et al

Poster (2011)

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See detailMacroH2A regulates the commitment of adult and embryonic stem cells to differentation
Creppe, Catherine ULg; Cantariño, N; Janich, P et al

Poster (2011)

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See detailImpacts de nos actions d’accompagnement d’enseignants en développement professionnel au sein du Master Complémentaire Formasup
Delfosse, Catherine ULg; Dupont, Chantal ULg; Jérôme, Françoise ULg et al

Poster (2011)

Présentations des modalités d'analyse du master complémentaire en pédagogie de l'enseignement supérieur pour le réguler.

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See detailAqueous and non-aqueous sol-gel preparation of TiO2 films for the photocatalytic oxidation of ethanol in air
Cimieri, Iolanda; Poelman, Hilde; Lambert, Stéphanie ULg et al

Poster (2011)

One of the most versatile methods to prepare TiO2 fine powders and films is the sol-gel technique. It offers many advantages, among which the possibility to control the stoichiometry, work in mild and ... [more ▼]

One of the most versatile methods to prepare TiO2 fine powders and films is the sol-gel technique. It offers many advantages, among which the possibility to control the stoichiometry, work in mild and ambient atmospheric conditions and achieve high purity and homogeneity of the final product. Stable TiO2 sols were synthesized by an aqueous sol-gel process using titanium tetraisopropoxide (TTIP) as precursor and nitric acid in water as hydrolyzing agent. The aim of the present study was to investigate the influence of different solvents and different amounts of HNO3 on the photocatalytic activity of TiO2 in the breakdown of ethanol (EtOH) as VOC molecule under UV and visible light. In particular, ethanol, isopropanol and butanol were used as solvents and two different molar ratios of HNO3 with respect to TTIP (0.25 and 0.5 respectively) were used. Their effect was investigated with regard to the photocatalytic properties of the films obtained by spin coating on sodium free glass [1] and heat treated at 450°C in air. The microstructure, optical and morphological properties of the films were investigated using SEM, UV-Vis spectroscopy and ellipsometry. The nature of the crystalline phases was ascertained by X-ray diffraction and the surface area of the powders was measured by N2 adsorption. The porous TiO2 films obtained show quite good photocatalytic activity for the degradation of EtOH compared to titania films prepared using P25 Degussa as shown in Fig. 1. Further the photocatalytic properties of the thin films obtained by aqueous sol-gel method were compared with the activity of films prepared using the same titanium precursor by a non-aqueous sol-gel process, in which the hydrolysis is initialized by esterification reaction between acetic acid and alcohol. (Fig.1. EtOH degradation for P25 (green), non aqueous sol (purple) and aqueous sols prepared using ethanol as solvent and HNO3 in molar ratios 0.25(blue) and 0.50(red)) [1] H. Tomaszewski, K. Eufinger, H. Poelman, D. Poelman, R. De Gryse, P.F.Smet, G.B. Marin, Int. J. Photoenergy 8, 1 (2007) [less ▲]

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See detailFirst assessment of the biogeochemistry of the Congo River and tributaries
Darchambeau, François ULg; Bouillon, S.; Wabakanghanzi, J. N. et al

Poster (2011)

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See detailSafety and Performance of Stochastic Targeted (STAR) Glycemic Control of Insulin and Nutrition - First Pilot Results
Shaw, Geoffrey M.; Le Compte, Aaron; Evans, Alicia et al

Poster (2011)

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See detailCaractérisation des pigments préhistoriques par analyse PIXE
Lebon, Matthieu; Beck, Lucile; Lahlil, Sophia et al

Poster (2011)

Detailed reference viewed: 21 (1 ULg)
See detailAn overview of the aims and plans of an ISSI Team: Investigating the Dynamics of Planetary Magnetotails
Jackman, C.; Andre, N.; Arridge, C. et al

Poster (2011)

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See detailSelf-DNA release mediates the adjuvant effects of aluminum salts
Marichal, Thomas ULg; Ohata, K; Bedoret, Denis et al

Poster (2011)

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See detailIs the PASAT enough to detect working memory problems in MS patients? A comparison with another working memory test
DELRUE, Gaël ULg; MOYANO, Nicolas; JIMENEZ et al

Poster (2011)

We show that the PASAT is not the best test to evaluate working memory in mulitple sclerosis patients.

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See detailReconstruction of satellite-derived sea surface temperature of the South China Sea in 2003-2009
Huynh, Thi Hong Ngu ULg; Alvera Azcarate, Aïda ULg; Beckers, Jean-Marie ULg

Poster (2011)

The South China Sea (SCS) is a large marginal sea in the tropical region where the percentage of missing data of the daily Advanced Very High Resolution Radiometer (AVHRR) sea surface temperature (SST) is ... [more ▼]

The South China Sea (SCS) is a large marginal sea in the tropical region where the percentage of missing data of the daily Advanced Very High Resolution Radiometer (AVHRR) sea surface temperature (SST) is very high. Here we use a relatively new technique, DINEOF (Data INterpolating Empirical Orthogonal Functions), to reconstruct the SST of the SCS in 2003-2009. Furthermore, a comparison between the reconstructed data and daily Tropical rainfall Measuring Mission Microwave Imager (TMI) SST is implemented. [less ▲]

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See detailThick and crack-free nanocrystalline mesoporous TiO2 films obtained by capillary coating from aqueous solutions
Krins, Natacha ULg; Faustini, Marco; Louis, Benjamin et al

Poster (2011)

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See detailGait feature extraction in Parkinson's disease using low-cost accelerometers
Stamatakis, Julien; Cremers, Julien ULg; Maquet, Didier ULg et al

Poster (2011)

The clinical hallmarks of Parkinson’s disease (PD) are movement poverty and slowness (i.e. bradykinesia), muscle rigidity, limb tremor or gait disturbances. Parkinson’s gait include slowness, shuffling ... [more ▼]

The clinical hallmarks of Parkinson’s disease (PD) are movement poverty and slowness (i.e. bradykinesia), muscle rigidity, limb tremor or gait disturbances. Parkinson’s gait include slowness, shuffling, short steps, freezing of gait (FoG) and/or asymmetries in gait. There are currently no validated clinical instruments or device that allow a full characterization of gait disturbances in PD. As a step towards this goal, a four accelerometer-based system is proposed to increase the number of parameters that can be extracted to characterize parkinsonian gait disturbances such as FoG or gait asymmetries. After developing the hardware, an algorithm has been developed, that automatically epoched the signals on a stride-by-stride basis and quantified, among others, the gait velocity, the stride time,the stance and swing phases, the single and double support phases or the maximum acceleration at toe-off, as validated by visual inspection of video recordings during the task. The results obtained in a PD patient and an healthy volunteer are presented. The FoG detection will be improved using time-frequency analysis and the system is about to be validated with a state-of-the-art 3D movement analysis system. [less ▲]

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See detailIs there really an Azores diversity enigma? Insights from the phylogeography of the heather Erica scoparia s.l.
Desamore, Aurélie ULg

Poster (2011)

The Azorean flora, in contrast to that of other archipelagos, is characterized by a paucity of evolutionary radiations and the widespread distribution of most endemics. Several hypotheses, including ... [more ▼]

The Azorean flora, in contrast to that of other archipelagos, is characterized by a paucity of evolutionary radiations and the widespread distribution of most endemics. Several hypotheses, including isolation from the continent, recent age, ecological homogeneity, and limited paleoclimatic variations, have been proposed to account for this pattern. These hypotheses are tested here within the context of the molecular phylogeography of the heather Erica scoparia s.l., a Mediterraneo-Atlantic species complex distributed across Macaronesia, North Africa and southwestern Europe. The marked molecular radiation of this species in the Azores, including an array of single-island endemics, suggests that the apparent homogeneity of the Azorean flora might, in fact, conceal undetected patterns of endemism that are similar to those observed in the Canarian flora for morphological data. Haplotype richness was higher in the Azores than in the Canaries and extensive dispersal mediated allopatry is apparent at the molecular level. The Azores were colonized at least twice independently by E. scoparia, whereas the Canarian haplotypes are monophyletic, suggesting that the greater distance between the Azores and the continent does not necessarily hamper the chances of successful colonization. [less ▲]

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See detailElectro-assisted formation of organothiols self-assembled monolayers on polycrystalline copper surfaces
Maho, Anthony ULg; Denayer, Jessica; Delhalle, Joseph et al

Poster (2011)

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See detailMesure de la lordose thoracique du porc : méthode centroïde versus méthode de Cobb
Van Cauwenberge, H; Georis, P; Busoni, Valeria ULg et al

Poster (2011)

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See detailThe influence of aromatic solvents on CuPC blue pigments in oil paintings
Defeyt, Catherine ULg; Wijnberg, Louise; Verbeeck, Muriel ULg et al

Poster (2011)

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See detailDynamic Magnetic Resonance Perfusion: Influence of the Cardiac Cycle on Left Ventricle and Myocardial Signal Intensity Measurements
BROUSSAUD, T; AOUCHRIA, A; MANCINI, ISABELLE et al

Poster (2011)

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See detailSub-wavelength quantum imaging using uncorrelated single photon sources
von Zanthier, J.; Thiel, C.; Bastin, Thierry ULg et al

Poster (2011)

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See detailComparative Planetary Magnetotails
Bagenal, F.; Jackman, C.; Slavin, J. et al

Poster (2011)

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Peer Reviewed
See detailSynthesis by sol-gel process of visible light sensitive-TiO2 for the degradation of pollutants and microorganisms
Tasseroul, Ludivine ULg; Lambert, Stéphanie ULg; Páez Martínez, Carlos ULg et al

Poster (2011)

Since the discovery of photocatalytic decomposition of water on TiO2 electrodes by Fujishima and Honda [1], heterogenous photocatalysis has been widely studied for environmental applications ... [more ▼]

Since the discovery of photocatalytic decomposition of water on TiO2 electrodes by Fujishima and Honda [1], heterogenous photocatalysis has been widely studied for environmental applications. Photocatalytic processes are new technologies for waste water treatment because new compounds (alkanes, pesticides, dyes, etc.) in effluents are not readily degraded by the conventional treatments [2]. Oxidative processes can completely destroy organic pollutants [3] or microorganisms (bacteria, viruses, fungi, etc.) [4,5]. At this moment, the commercial Degussa P25 is the mostly used photocatalyst. Nevertheless, it requires UV light to be activated, that is why scientists try to activate TiO2 under visible light. The aim of this study is to synthesize photocatalysts activated by visible light and to evaluate their activity for the degradation of pollutants and microorganisms. The sol-gel process allows the direct introduction of visible light sensitive species like porphyrins inside the TiO2 matrix during the synthesis [6]. Two tetracarboxyphenyl porphyrins are synthesized for the sensitization of TiO2 to visible light : the metal free tetracarboxyphenyl porphyrin and the nickel tetracarboxyphenyl porphyrin. These porphyrins are characterized by 1H NMR, FT-IR and UV/Vis spectroscopies. They are introduced during the sol-gel synthesis of TiO2 matrix. Diffuse reflectance and FT-IR spectroscopies are used to ensure that porphyrins are incorporated inside the matrix of TiO2. Indeed, characteristic porphyrins peaks appear on spectra. The cristallinity and specific surfaces of catalysts are also determined by XRD and nitrogen adsorption-desorption measurements respectively. The depolluting efficiency of photocatalysts is evaluated for the degradation in water of p-nitrophenol, which is listed as one of the 114 organic pollutants by the Environmental Protection Agency in the US. After 6 h under visible light, 40 % of p-nitrophenol are degraded with some catalysts. The activity is influenced by i) the presence of porphyrins : the photoactivity increases with the amount of porphyrins ; ii) the nature of porphyrins : nickel porphyrins are more active than free metal porphyrins ; and iii) the crystallinity of catalysts : amorphous catalysts are less active than crystallized samples. The photocatalysts with the highest degradation rating of dyes will be selected for the degradation in water of Escherichia coli and Lactobacillus acidophilus. [1] A. Fujishima, K. Honda, Nature 238, 37-38 (1972) [2] P. Gogate, A. Pandit, Advances in Environmental Research 8, 501-551 (2004). [3] D. Chen, A. Ray, Water Research 32, 3223-3234 (1998) [4] R. Watts, S. Kong, M. Orr, G. Miller, B. Henry, Water Research 29, 95-100 (1995). [5] J. Hong, M. Otaki, Journal of Bioscience and Bioengineering 101, 185-189 (2006). [6] C. Wang, J. Li, G. Mele, G.M. Yang, F.-X. Zhang, L. Palmisano, G. Vasapollo, Applied catalysis 78, 218-226 (2007). [less ▲]

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See detailEndogenous zinc modulates di-synaptic GABAergic transmission in the dentate gyrus
Grauert, A; Engel, Dominique ULg; Ruiz, AJ

Poster (2011)

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See detailDistribution of Colonic Findings at Virtual Colonoscopy According to Gastrointestinal Symptoms in Almost 10,000 Patients
HOCK, D; OUHADI, R; MATERNE, R et al

Poster (2011)

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See detailPreliminary results of financial analysis on poultry supply chains in Hanoi suburb, North-Vietnam
Phan Dang, Thang; Vu Dinh, Ton; Dogot, Thomas ULg et al

Poster (2011)

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See detailPEB: thermal oriented architectural modeling for building energy efficiency regulations
Leclercq, Pierre ULg; Juchmes, Roland ULg; Delfosse, Vincent ULg et al

Poster (2011)

As part of the overhauling of the building energy efficiency regulations (following European directive 2002/91/CE), the Wallonia and Brussels-Capital Region commissioned the LUCID to develop an optional ... [more ▼]

As part of the overhauling of the building energy efficiency regulations (following European directive 2002/91/CE), the Wallonia and Brussels-Capital Region commissioned the LUCID to develop an optional 3D graphic encoding module to be integrated with the core energy efficiency computation engine developed by Altran Europe. Our contribution consisted mostly in analyzing the target users’ needs and representations (ergonomics, UI, interactions) and implementing a bespoke 3D CAD modeler dedicated to thermal models of buildings taking into account the various geometrical and relational constraints specific to thermal modeling. [less ▲]

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See detailIdentification and characterization of novel galectin-9 splice variants in endothelial cells
Heusschen, Roy ULg; De Bree, Martijn; Griffioen, Arjan et al

Poster (2011)

Detailed reference viewed: 28 (7 ULg)
See detailRapid regulation by glutamate of aromatase activity
Charlier, Thierry ULg; Ball, Gregory; Balthazart, Jacques ULg

Poster (2011)

Detailed reference viewed: 22 (0 ULg)